Susan K Buchanan
Affiliation: National Institutes of Health
Noinaj N, Rollauer S, Buchanan S. The Î²-barrel membrane protein insertase machinery from Gram-negative bacteria. Curr Opin Struct Biol. 2015;31:35-42 pubmed publisher
Kuszak A, Jacobs D, Gurnev P, Shiota T, Louis J, Lithgow T, et al
. Evidence of Distinct Channel Conformations and Substrate Binding Affinities for the Mitochondrial Outer Membrane Protein Translocase Pore Tom40. J Biol Chem. 2015;290:26204-17 pubmed publisher
..These results provide the first evidence for structurally distinct Tom40 conformations playing a role in substrate recognition and therefore in transport function. ..
Kuszak A, Noinaj N, Buchanan S. Methods to Characterize Folding and Function of BamA Cross-Link Mutants. Methods Mol Biol. 2015;1329:137-47 pubmed publisher
..This chapter summarizes efforts as applied to the disulfide cross-link mutants of BamA and describes experimental methods used in our studies to determine that lateral opening of the barrel domain is required for function. ..
Noinaj N, Buchanan S. Summary and Future Directions. Methods Mol Biol. 2015;1329:279-80 pubmed publisher
..It is anticipated that the next decade will further advance our knowledge of how the BAM complex is able to perform its unique and interesting function. ..
Noinaj N, Buchanan S. Structural insights into the transport of small molecules across membranes. Curr Opin Struct Biol. 2014;27:8-15 pubmed publisher
Noinaj N, Kuszak A, Balusek C, GUMBART J, Buchanan S. Lateral opening and exit pore formation are required for BamA function. Structure. 2014;22:1055-62 pubmed publisher
..These data provide strong evidence that lateral opening and exit pore formation are required for BamA function. ..
Rollauer S, Sooreshjani M, Noinaj N, Buchanan S. Outer membrane protein biogenesis in Gram-negative bacteria. Philos Trans R Soc Lond B Biol Sci. 2015;370: pubmed publisher
..Here, we will review some of these recent discoveries that have advanced our understanding of the biogenesis of OMPs in Gram-negative bacteria, starting with synthesis in the cytoplasm to folding and insertion into the OM. ..
Buchanan S, Lukacik P, Grizot S, Ghirlando R, Ali M, Barnard T, et al
. Structure of colicin I receptor bound to the R-domain of colicin Ia: implications for protein import. EMBO J. 2007;26:2594-604 pubmed
..Functional data using full-length colicin Ia show that colicin I receptor is necessary for cell surface binding, and suggest that the receptor participates in translocation of colicin Ia across the outer membrane. ..
Buchanan S. Bacterial metal detectors. Mol Microbiol. 2005;58:1205-9 pubmed
..The new structure extends our knowledge of transporter architecture and suggests how signalling may occur across the outer membrane. ..