K E Brown

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Rhesus and pig-tailed macaque parvoviruses: identification of two new members of the erythrovirus genus in monkeys
    S W Green
    Hematology Branch, National Heart, Lung and Blood Institute, Bethesda, MD 20892, USA
    Virology 269:105-12. 2000
  2. ncbi request reprint Simian parvovirus infection: a potential zoonosis
    Kevin E Brown
    Hematology Branch, National Heart, Lung, and Blood Institute, Building 10, Room CRC 3 5130, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892 1202, USA
    J Infect Dis 190:1900-7. 2004
  3. doi request reprint VP1u phospholipase activity is critical for infectivity of full-length parvovirus B19 genomic clones
    Claudia Filippone
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institute of Health, Bethesda, MD 20892, USA
    Virology 374:444-52. 2008
  4. ncbi request reprint Variants of B19
    K E Brown
    Hematology Branch, National Heart, Lung and Blood Institute, Bethesda, MD 20892, USA
    Dev Biol (Basel) 118:71-7. 2004
  5. ncbi request reprint Transcription-positive cofactor 4 enhances rescue of adeno-associated virus genome from an infectious clone
    S Muramatsu
    Hematology Branch, National Heart, Lung and Blood Institute, Bethesda, MD 20892 1652, USA
    J Gen Virol 79:2157-61. 1998
  6. ncbi request reprint Erythrocyte P antigen: cellular receptor for B19 parvovirus
    K E Brown
    Hematology Branch, National Heart, Lung and Blood Institute, Bethesda, MD 20892
    Science 262:114-7. 1993
  7. ncbi request reprint The interaction of heparin sulfate and adeno-associated virus 2
    J Qiu
    Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Virology 269:137-47. 2000
  8. ncbi request reprint GB virus C/Hepatitis G virus infection is frequent in American children and young adults
    A Handa
    Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA
    Clin Infect Dis 30:569-71. 2000
  9. ncbi request reprint Adeno-associated virus (AAV)-3-based vectors transduce haematopoietic cells not susceptible to transduction with AAV-2-based vectors
    A Handa
    Hematology Branch, National Heart, Lung and Blood Institute, Bldg 10 Rm 7C218, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892 1652, USA
    J Gen Virol 81:2077-84. 2000
  10. ncbi request reprint Prevalence of the newly described human circovirus, TTV, in United States blood donors
    A Handa
    Hematology Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD 20892, USA
    Transfusion 40:245-51. 2000

Collaborators

Detail Information

Publications40

  1. ncbi request reprint Rhesus and pig-tailed macaque parvoviruses: identification of two new members of the erythrovirus genus in monkeys
    S W Green
    Hematology Branch, National Heart, Lung and Blood Institute, Bethesda, MD 20892, USA
    Virology 269:105-12. 2000
    ..Both viruses have been cloned and over 5000 nucleotides sequenced from each virus. The viruses show marked similarities to other members of the Erythrovirus genus in the Parvoviridae family...
  2. ncbi request reprint Simian parvovirus infection: a potential zoonosis
    Kevin E Brown
    Hematology Branch, National Heart, Lung, and Blood Institute, Building 10, Room CRC 3 5130, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892 1202, USA
    J Infect Dis 190:1900-7. 2004
    ..We hypothesized that SPV may infect humans and replicate in human bone marrow mononuclear cells...
  3. doi request reprint VP1u phospholipase activity is critical for infectivity of full-length parvovirus B19 genomic clones
    Claudia Filippone
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institute of Health, Bethesda, MD 20892, USA
    Virology 374:444-52. 2008
    ..Investigation of the original serum from which pB19-FL was cloned confirmed that the phospholipase mutation was present in the native B19 virus...
  4. ncbi request reprint Variants of B19
    K E Brown
    Hematology Branch, National Heart, Lung and Blood Institute, Bethesda, MD 20892, USA
    Dev Biol (Basel) 118:71-7. 2004
    ..This review will give an overview of the biology of parvovirus B19 infection, and the more recently described variants of parvovirus B19...
  5. ncbi request reprint Transcription-positive cofactor 4 enhances rescue of adeno-associated virus genome from an infectious clone
    S Muramatsu
    Hematology Branch, National Heart, Lung and Blood Institute, Bethesda, MD 20892 1652, USA
    J Gen Virol 79:2157-61. 1998
    ..A significantly increased number of AAV-3 genomes were rescued in PC4 transfected cells. Our results support a possible involvement of PC4 in AAV replication and may be used in efficient production of AAV vectors for gene therapy...
  6. ncbi request reprint Erythrocyte P antigen: cellular receptor for B19 parvovirus
    K E Brown
    Hematology Branch, National Heart, Lung and Blood Institute, Bethesda, MD 20892
    Science 262:114-7. 1993
    ..Knowledge of a parvovirus receptor has implications for understanding the pathogenesis of parvovirus infections and for the use of parvoviruses in gene therapy...
  7. ncbi request reprint The interaction of heparin sulfate and adeno-associated virus 2
    J Qiu
    Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Virology 269:137-47. 2000
    ..Overall, our data suggest that, as has been shown for other viruses, the presence of a high-affinity AAV2 receptor mediates AAV2 infection in addition to the low-affinity heparan sulfate binding...
  8. ncbi request reprint GB virus C/Hepatitis G virus infection is frequent in American children and young adults
    A Handa
    Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA
    Clin Infect Dis 30:569-71. 2000
    ..8% of subjects, indicating that GBV-C/HGV infection appears to be common among American children and young adults, even in the absence of blood transfusion...
  9. ncbi request reprint Adeno-associated virus (AAV)-3-based vectors transduce haematopoietic cells not susceptible to transduction with AAV-2-based vectors
    A Handa
    Hematology Branch, National Heart, Lung and Blood Institute, Bldg 10 Rm 7C218, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892 1652, USA
    J Gen Virol 81:2077-84. 2000
    ..These results suggest not only that there are different cellular receptors for AAV-2 and AAV-3, but that rAAV-3 vectors may be preferred for transduction of some haematopoietic cell types...
  10. ncbi request reprint Prevalence of the newly described human circovirus, TTV, in United States blood donors
    A Handa
    Hematology Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD 20892, USA
    Transfusion 40:245-51. 2000
    ..A novel nonenveloped single-stranded circular DNA virus (TTV) was recently identified. The prevalence of TTV in blood donors in the United States is, however, still unclear...
  11. ncbi request reprint Integrin alphaVbeta5 is not involved in adeno-associated virus type 2 (AAV2) infection
    J Qiu
    Hematology Branch, National Heart, Lung and Blood Institute, Bethesda, Maryland, 20892, USA
    Virology 264:436-40. 1999
    ..Furthermore, neither purified integrin alphaVbeta5, nor RGD peptides, nor functional blocking monoclonal antibody blocked rAAV2 transduction. These data strongly suggest that integrin alphaVbeta5 is not involved in AAV2 infection...
  12. ncbi request reprint A 110-kDa nuclear shuttle protein, nucleolin, specifically binds to adeno-associated virus type 2 (AAV-2) capsid
    J Qiu
    National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Virology 257:373-82. 1999
    ....
  13. ncbi request reprint Sequencing and characterization of A-2 plaque virus: A new member of the Picornaviridae family
    Z Liu
    Hematology Branch, National Institutes of Health, Bethesda, Maryland 20892 1642, USA
    Virology 272:168-76. 2000
    ..These results suggest that A2 virus is a new member of the Picornaviridae but that its pathogenicity in nonhuman primates and association with human disease still need to be determined...
  14. ncbi request reprint Resistance to parvovirus B19 infection due to lack of virus receptor (erythrocyte P antigen)
    K E Brown
    Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892
    N Engl J Med 330:1192-6. 1994
    ..CONCLUSIONS. People who do not have P antigen, which is the cellular receptor for parvovirus B19, are naturally resistant to infection with this pathogen...
  15. ncbi request reprint GB virus C/hepatitis G virus replicates in human haematopoietic cells and vascular endothelial cells
    A Handa
    Hematology Branch, National Heart, Lung and Blood Institute, Bldg 10 Rm 7C218, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892 1652, USA
    J Gen Virol 81:2461-9. 2000
    ....
  16. ncbi request reprint Construction and sequencing of an infectious clone of the human parvovirus B19
    Ning Zhi
    Hematology Branch, National Heart Lung and Blood Institute, Bethesda, MD, USA
    Virology 318:142-52. 2004
    ..Together, these results indicate the first successful production of an infectious clone for parvovirus B19 virus...
  17. ncbi request reprint Parvovirus B19
    Neal S Young
    Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892 1652, USA
    N Engl J Med 350:586-97. 2004
  18. pmc Molecular and functional analyses of a human parvovirus B19 infectious clone demonstrates essential roles for NS1, VP1, and the 11-kilodalton protein in virus replication and infectivity
    Ning Zhi
    Hematology Branch, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1202, USA
    J Virol 80:5941-50. 2006
    ..These findings suggest a previously unrecognized role for the 11-kDa protein, and together the results enhance our understanding of the key features of the B19 viral genome and proteins...
  19. ncbi request reprint Increased frequency of HLA-DR2 in patients with paroxysmal nocturnal hemoglobinuria and the PNH/aplastic anemia syndrome
    J P Maciejewski
    Hematology Branch and Office of Biostatistics Research, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 98:3513-9. 2001
    ..The results suggest that clonal expansion of GPI-AP-deficient cells is linked to HLA and likely related to an immune mechanism...
  20. ncbi request reprint Haematological consequences of parvovirus B19 infection
    K E Brown
    Hematology Branch, National Heart, Lung and Blood Institute, Bethesda, Maryland, USA
    Baillieres Best Pract Res Clin Haematol 13:245-59. 2000
    ..Diagnosis is based on examination of bone marrow and B19 virological studies. Treatment of persistent infection with immunoglobulin leads to a prompt resolution of the anaemia...
  21. ncbi request reprint Increased expression of tissue inhibitor of metalloproteinases type 1 (TIMP-1) in a more tumourigenic colon cancer cell line
    R E Hewitt
    Laboratory of Pathology, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA
    J Pathol 192:455-9. 2000
    ..This association between TIMP-1 expression and tumour progression may be related to additional growth factor-like effects described for TIMP-1 in some systems...
  22. ncbi request reprint Parvovirus B19: implications for transfusion medicine. Summary of a workshop
    K E Brown
    National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Transfusion 41:130-5. 2001
    ..If clinical trials were to be developed, participants recommended that they include special risk groups such as seronegative pregnant women and children with malignancies who are receiving chemotherapy...
  23. pmc Adeno-associated virus serotype 4 (AAV4) and AAV5 both require sialic acid binding for hemagglutination and efficient transduction but differ in sialic acid linkage specificity
    N Kaludov
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, USA
    J Virol 75:6884-93. 2001
    ..These results suggest that AAV4 and AAV5 require different sialic acid-containing glycoproteins for binding and transduction of target cells and they further explain the different tropism of AAV4 and AAV5...
  24. ncbi request reprint Development of an improved method of detection of infectious parvovirus B19
    Susan Wong
    Hematology Branch, National Heart, Lung and Blood Institute, Bethesda, MD, USA
    J Clin Virol 35:407-13. 2006
    ..B19 is only cultured with difficulty in vitro, and there is no readily available assay for detecting B19 infectivity or neutralizing antibodies...
  25. ncbi request reprint Nucleotide sequencing and generation of an infectious clone of adeno-associated virus 3
    S Muramatsu
    Hematology Branch, National Heart, Lung and Blood Institute, Bethesda, Maryland 20892 1652, USA
    Virology 221:208-17. 1996
    ..The infectious clone confirmed the sequence and may be useful for developing new vectors for gene therapy...
  26. ncbi request reprint Rabaptin-5 is a novel fusion partner to platelet-derived growth factor beta receptor in chronic myelomonocytic leukemia
    M K Magnusson
    Hematology Branch, National Heart, Lung, and Blood Institute, and Laboratory of Pathology, Division of Clinical Sciences, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    Blood 98:2518-25. 2001
    ..Early endosomal transport is critical in regulation of various growth factor receptors, through ligand-induced clathrin-mediated endocytosis, and thus this new fusion protein links together 2 important pathways of growth regulation...
  27. ncbi request reprint Prevalence of parvovirus B19 in liver tissue: no association with fulminant hepatitis or hepatitis-associated aplastic anemia
    Susan Wong
    Virus Discovery Group, Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Infect Dis 187:1581-6. 2003
    ..There was a significant increase (P<.1) in the prevalence of variant erythrovirus sequences in livers of patients with HBV or HCV hepatitis, the reason for which is currently unknown...
  28. pmc Ex vivo-generated CD36+ erythroid progenitors are highly permissive to human parvovirus B19 replication
    Susan Wong
    Hematology Branch, NHLBI, NIH, Bldg 10 CRC, Rm 3E 5216, 10 Center Drive, Bethesda, MD 20892, USA
    J Virol 82:2470-6. 2008
    ..This is the first description of an ex vivo method to produce large numbers of EPCs that are highly permissive to B19V infection and replication, offering a cellular system that mimics in vivo infection with this pathogenic human virus...
  29. ncbi request reprint Differential mRNA regulation of integrin subunits alpha V, beta 1, beta 3, and beta 5 during mouse embryonic organogenesis
    S Yamada
    Laboratory of Developmental Biology, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892, USA
    Cell Adhes Commun 3:311-25. 1995
    ..The beta 5 integrin is unique in its degree of tissue-specific mRNA regulation associated with morphogenesis of embryonic organs...
  30. ncbi request reprint Integrin regulatory switching in development: oscillation of beta 5 integrin mRNA expression during epithelial-mesenchymal interactions in tooth development
    S Yamada
    Laboratory of Developmental Biology, National Institute of Dental Research, National Institutes of Health, Bethesda
    Int J Dev Biol 38:553-6. 1994
    ..This rapid modulation of mRNA expression suggests a potential regulatory role for the beta 5 integrin receptor in morphogenesis...
  31. ncbi request reprint Activation of synoviocytes by the secreted phospholipase A2 motif in the VP1-unique region of parvovirus B19 minor capsid protein
    Jun Lu
    Hematology Branch, National Heart, Lung, and Blood, Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Infect Dis 193:582-90. 2006
    ..Proteins with VP1u in noninfectious B19 may participate in the inflammatory response in the synovial compartment...
  32. pmc Splice junction map of simian parvovirus transcripts
    Kapil Vashisht
    Department of Veterinary Pathology, University of Minnesota, St Paul, MN, USA
    J Virol 78:10911-9. 2004
    ..The findings indicate that that the splicing pattern of transcripts of SPV and B19 is similar, but SPV also has coding strategies in common with other parvoviruses...
  33. ncbi request reprint Evaluation of a new LightCycler reverse transcription-polymerase chain reaction infectivity assay for detection of human parvovirus B19 in dry-heat inactivation studies
    Grigori G Prikhod'ko
    Plasma Derivatives Department, Jerome H Holland Laboratory for the Biomedical Sciences, American Red Cross, Rockville, Maryland 20855, USA
    Transfusion 45:1011-9. 2005
    ..Fifth disease has been reported in patients receiving red blood cells, platelets, solvent/detergent-treated plasma, and clotting factor concentrates...
  34. ncbi request reprint Parvovirus B19 and onset of juvenile dermatomyositis
    Gulnara Mamyrova
    JAMA 294:2170-1. 2005
  35. ncbi request reprint Identification and characterization of a second novel human erythrovirus variant, A6
    Quang Tri Nguyen
    Institute of Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark
    Virology 301:374-80. 2002
    ..Current PCR analyses are unlikely to detect novel variants without the design of specific primers to the A6/V9/B19 common sequences...
  36. pmc Human parvovirus B19
    Erik D Heegaard
    Department of Clinical Microbiology, University State Hospital, Rigshospitalet, Copenhagen, Denmark
    Clin Microbiol Rev 15:485-505. 2002
    ..Treatment of persistent infection with immunoglobulin reduces the viral load and results in a marked resolution of anemia. Vaccine phase I trials show promising results...
  37. ncbi request reprint Parvoviruses and blood transfusion
    Kevin E Brown
    Transfusion 47:1745-50. 2007
  38. ncbi request reprint Detection and quantitation of parvovirus B19
    Kevin E Brown
    J Clin Virol 31:1-4. 2004
  39. ncbi request reprint Increased prevalence of transfusion-transmitted virus and cross-reactivity with immunodominant epitopes of the HRES-1/p28 endogenous retroviral autoantigen in patients with systemic lupus erythematosus
    Peter Gergely
    Section of Rheumatology, Department of Medicine, State University of New York, Upstate Medical University, College of Medicine, Syracuse, NY 13210, USA
    Clin Immunol 116:124-34. 2005
    ..To identify cross-reactive viral antigens capable of triggering autoreactivity, HRES-1/p28 epitopes were mapped by SLE antibodies...
  40. pmc Comparison of the transcription profile of simian parvovirus with that of the human erythrovirus B19 reveals a number of unique features
    Zhengwen Liu
    M621 Medical Sciences Bldg, School of Medicine, University of Missouri Columbia, Columbia, MO 65212, USA
    J Virol 78:12929-39. 2004
    ..5-kDa protein, and SPV NS1, respectively). The 14-kDa protein was localized to both in the nucleus and cytoplasm...