R M Brosh

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Biochemical characterization of the DNA substrate specificity of Werner syndrome helicase
    Robert M Brosh
    Laboratory of Molecular Gerontology, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 277:23236-45. 2002
  2. ncbi request reprint Biochemical characterization of the WRN-FEN-1 functional interaction
    Robert M Brosh
    Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Biochemistry 41:12204-16. 2002
  3. pmc Werner syndrome protein interacts with human flap endonuclease 1 and stimulates its cleavage activity
    R M Brosh
    Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    EMBO J 20:5791-801. 2001
  4. ncbi request reprint DNA repair helicases as targets for anti-cancer therapy
    Rigu Gupta
    Laboratory of Molecular Gerontology, National Institute on Aging, NIH, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Curr Med Chem 14:503-17. 2007
  5. ncbi request reprint Coordinate action of the helicase and 3' to 5' exonuclease of Werner syndrome protein
    P L Opresko
    Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 276:44677-87. 2001
  6. pmc Potent inhibition of werner and bloom helicases by DNA minor groove binding drugs
    R M Brosh
    Laboratory of Molecular Genetics, Box 1, National Institute on Aging, GRC, NIH, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Nucleic Acids Res 28:2420-30. 2000
  7. ncbi request reprint Replication protein A physically interacts with the Bloom's syndrome protein and stimulates its helicase activity
    R M Brosh
    Laboratory of Molecular Genetics, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 275:23500-8. 2000
  8. pmc Ku complex interacts with and stimulates the Werner protein
    M P Cooper
    Laboratory of Molecular Genetics, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA
    Genes Dev 14:907-12. 2000
  9. ncbi request reprint Stimulation of flap endonuclease-1 by the Bloom's syndrome protein
    Sudha Sharma
    Laboratory of Molecular Gerontology, NIA, National Institutes of Health, DHHS, Baltimore, Maryland 21224 6825, USA
    J Biol Chem 279:9847-56. 2004
  10. ncbi request reprint p53 Modulates the exonuclease activity of Werner syndrome protein
    R M Brosh
    Laboratory of Molecular Gerontology, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 276:35093-102. 2001

Collaborators

Detail Information

Publications68

  1. ncbi request reprint Biochemical characterization of the DNA substrate specificity of Werner syndrome helicase
    Robert M Brosh
    Laboratory of Molecular Gerontology, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 277:23236-45. 2002
    ..The ability of WRN to target DNA replication/repair intermediates may be relevant to its role in genome stability maintenance...
  2. ncbi request reprint Biochemical characterization of the WRN-FEN-1 functional interaction
    Robert M Brosh
    Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Biochemistry 41:12204-16. 2002
    ..The ability of WRN to facilitate FEN-1 cleavage of DNA replication/repair intermediates may be important for the role of WRN in the maintenance of genomic stability...
  3. pmc Werner syndrome protein interacts with human flap endonuclease 1 and stimulates its cleavage activity
    R M Brosh
    Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    EMBO J 20:5791-801. 2001
    ..The underlying defect of WS is discussed in light of the evidence for the interaction between WRN and FEN-1...
  4. ncbi request reprint DNA repair helicases as targets for anti-cancer therapy
    Rigu Gupta
    Laboratory of Molecular Gerontology, National Institute on Aging, NIH, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Curr Med Chem 14:503-17. 2007
    ....
  5. ncbi request reprint Coordinate action of the helicase and 3' to 5' exonuclease of Werner syndrome protein
    P L Opresko
    Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 276:44677-87. 2001
    ..RPA stimulated the WRN helicase, whereas Ku stimulated the WRN exonuclease. In the presence of both RPA and Ku, the WRN helicase activity dominated the exonuclease activity...
  6. pmc Potent inhibition of werner and bloom helicases by DNA minor groove binding drugs
    R M Brosh
    Laboratory of Molecular Genetics, Box 1, National Institute on Aging, GRC, NIH, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Nucleic Acids Res 28:2420-30. 2000
    ..The distinct inhibition of WRN and BLM helicases by the minor groove binders suggest that these helicases unwind double-stranded DNA by a related mechanism...
  7. ncbi request reprint Replication protein A physically interacts with the Bloom's syndrome protein and stimulates its helicase activity
    R M Brosh
    Laboratory of Molecular Genetics, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 275:23500-8. 2000
    ..The interactions between BLM and hRPA suggest that the two proteins function together in vivo to unwind DNA duplexes during replication, recombination, or repair...
  8. pmc Ku complex interacts with and stimulates the Werner protein
    M P Cooper
    Laboratory of Molecular Genetics, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA
    Genes Dev 14:907-12. 2000
    ..Although Ku proteins had no effect on ATPase or helicase activity, they strongly stimulated specific exonuclease activity. These results suggest that WRNp and the Ku complex participate in a common DNA metabolic pathway...
  9. ncbi request reprint Stimulation of flap endonuclease-1 by the Bloom's syndrome protein
    Sudha Sharma
    Laboratory of Molecular Gerontology, NIA, National Institutes of Health, DHHS, Baltimore, Maryland 21224 6825, USA
    J Biol Chem 279:9847-56. 2004
    ..We suggest that functional interactions between RecQ helicases and Rad2 family nucleases serve to process DNA substrates that are intermediates in DNA replication and repair...
  10. ncbi request reprint p53 Modulates the exonuclease activity of Werner syndrome protein
    R M Brosh
    Laboratory of Molecular Gerontology, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 276:35093-102. 2001
    ..The regulation of WRN function by p53 is likely to play an important role in the maintenance of genomic integrity and prevention of cancer and other clinical symptoms associated with WS...
  11. ncbi request reprint The Cockayne Syndrome group B gene product is involved in general genome base excision repair of 8-hydroxyguanine in DNA
    J Tuo
    Laboratory of Molecular Gerontology, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 276:45772-9. 2001
    ..The biological functions of CSB in different DNA repair pathways may be mediated by distinct functional motifs of the protein...
  12. ncbi request reprint Unwinding of a DNA triple helix by the Werner and Bloom syndrome helicases
    R M Brosh
    Laboratory of Molecular Genetics, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 276:3024-30. 2001
    ..It seems likely that structural features of the triplex mimic those of a fork and thus support efficient unwinding by the two helicases...
  13. ncbi request reprint The exonucleolytic and endonucleolytic cleavage activities of human exonuclease 1 are stimulated by an interaction with the carboxyl-terminal region of the Werner syndrome protein
    Sudha Sharma
    Laboratory of Molecular Gerontology, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 278:23487-96. 2003
    ..Thus, the genomic instability observed in WRN-/- cells may be at least partially attributed to the lack of interactions between the WRN protein and human nucleases including EXO-1...
  14. ncbi request reprint DNA repair and mutagenesis in Werner syndrome
    V A Bohr
    Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA
    Environ Mol Mutagen 38:227-34. 2001
    ..Whereas cellular studies so far do not show significant DNA repair deficiencies, biochemical studies with the Werner protein clearly indicate that it plays a role in DNA repair...
  15. pmc WRN helicase and FEN-1 form a complex upon replication arrest and together process branchmigrating DNA structures associated with the replication fork
    Sudha Sharma
    Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA
    Mol Biol Cell 15:734-50. 2004
    ..These results provide evidence for an interaction between WRN and FEN-1 in vivo and suggest that these proteins function together to process DNA structures associated with the replication fork...
  16. ncbi request reprint p53 modulates RPA-dependent and RPA-independent WRN helicase activity
    Joshua A Sommers
    Laboratory of Molecular Gerontology, National Institute on Aging, NIH, Baltimore, Maryland, USA
    Cancer Res 65:1223-33. 2005
    ..Regulation of WRN helicase activity by p53 is likely to play an important role in genomic integrity surveillance, a vital function in the prevention of tumor progression...
  17. ncbi request reprint DNA helicases as targets for anti-cancer drugs
    Sudha Sharma
    Laboratory of Molecular Gerontology, National Institute on Aging, NIH, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Curr Med Chem Anticancer Agents 5:183-99. 2005
    ....
  18. ncbi request reprint Functional and physical interaction between WRN helicase and human replication protein A
    R M Brosh
    Laboratory of Molecular Genetics, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 274:18341-50. 1999
    ..The physical and functional interaction between WRN and hRPA suggests that the two proteins may function together in vivo in a pathway of DNA metabolism such as replication, recombination, or repair...
  19. ncbi request reprint RECQ1 helicase interacts with human mismatch repair factors that regulate genetic recombination
    Kevin M Doherty
    Laboratory of Molecular Gerontology, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 280:28085-94. 2005
    ..Regulation of genetic recombination, a proposed role for RecQ helicases, is supported by the identified RECQ1 protein interactions and is discussed...
  20. pmc The ATPase domain but not the acidic region of Cockayne syndrome group B gene product is essential for DNA repair
    R M Brosh
    Laboratory of Molecular Genetics, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA
    Mol Biol Cell 10:3583-94. 1999
    ..Thus the acidic region of CSB is likely to be dispensable for DNA repair, whereas the ATPase domain is essential for CSB function in both TCR-dependent and -independent pathways...
  21. ncbi request reprint Processing of DNA replication and repair intermediates by the concerted action of RecQ helicases and Rad2 structure-specific nucleases
    Sudha Sharma
    Laboratory of Molecular Gerontology, National Institute on Aging, NIH, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Protein Pept Lett 15:89-102. 2008
    ....
  22. ncbi request reprint Biochemical and kinetic characterization of the DNA helicase and exonuclease activities of werner syndrome protein
    Saba Choudhary
    Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    J Biol Chem 279:34603-13. 2004
    ..These are the first reported kinetic parameters of a human RecQ unwinding reaction based on real time measurements, and they provide mechanistic insights into WRN-catalyzed DNA unwinding...
  23. ncbi request reprint In vivo function of the conserved non-catalytic domain of Werner syndrome helicase in DNA replication
    Sudha Sharma
    Laboratory of Molecular Gerontology, National Institute on Aging, NIH, 5600 Nathan Shock Drive, Baltimore, Maryland 21224, USA
    Hum Mol Genet 13:2247-61. 2004
    ....
  24. ncbi request reprint Physical and functional mapping of the replication protein a interaction domain of the werner and bloom syndrome helicases
    Kevin M Doherty
    Laboratory of Molecular Gerontology, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 280:29494-505. 2005
    ..These results suggest that the physical interaction between RPA and WRN or BLM helicases plays an important role in the mechanism for RPA stimulation of helicase-catalyzed DNA unwinding...
  25. ncbi request reprint Biochemical analysis of the DNA unwinding and strand annealing activities catalyzed by human RECQ1
    Sudha Sharma
    Laboratory of Molecular Gerontology, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 280:28072-84. 2005
    ..The enzymatic properties of the RECQ1 helicase and strand annealing activities are discussed in the context of proposed cellular DNA metabolic pathways that are important in the maintenance of genomic stability...
  26. ncbi request reprint Modulation of Werner syndrome protein function by a single mutation in the conserved RecQ domain
    Jae Wan Lee
    Laboratory of Molecular Gerontology, NIA, National Institutes of Health, Baltimore, Maryland 21224 6825, USA
    J Biol Chem 280:39627-36. 2005
    ..Our nuclear magnetic resonance data on the three-dimensional structure of the wild-type RQC and Lys-1016 mutant proteins display a remarkable similarity in their structures...
  27. ncbi request reprint Inhibition of Werner syndrome helicase activity by benzo[a]pyrene diol epoxide adducts can be overcome by replication protein A
    Saba Choudhary
    Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health DHHS, Baltimore, MD 21224, USA
    J Biol Chem 281:6000-9. 2006
    ....
  28. ncbi request reprint Pathways defective in the human premature aging disease Werner syndrome
    Vilhelm A Bohr
    Laboratory of Molecular Gerontology, National Institute on Aging, NIH, Baltimore, MD 21224, USA
    Biogerontology 3:89-94. 2002
    ..The function and role of this protein is discussed in the light of how it functions in the aging process...
  29. pmc Welcome the family of FANCJ-like helicases to the block of genome stability maintenance proteins
    Y Wu
    Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, NIH Biomedical Research Center, 251 Bayview Blvd, Baltimore, MD 21224, USA
    Cell Mol Life Sci 66:1209-22. 2009
    ..The recently solved structure of XPD has provided new insight to the helicase core and accessory domains of sequence related Superfamily 2 helicases. The functions and roles of members of the FANCJ-like helicase family will be discussed...
  30. pmc Hitting the bull's eye: novel directed cancer therapy through helicase-targeted synthetic lethality
    Monika Aggarwal
    Laboratory of Molecular Gerontology, National Institute on Aging, NIH, NIH Biomedical Research Center, 251 Bayview Drive, Baltimore, Maryland 21224, USA
    J Cell Biochem 106:758-63. 2009
    ..In this review, we discuss this hypothesis and current evidence for synthetic lethal interactions of eukaryotic DNA helicases in model systems...
  31. pmc Human premature aging, DNA repair and RecQ helicases
    Robert M Brosh
    Laboratory of Molecular Gerontology, National Institute on Aging, NIH, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Nucleic Acids Res 35:7527-44. 2007
    ..We will also discuss the clinical features of the premature aging disorders associated with RecQ helicase deficiencies and how they relate to the molecular defects...
  32. ncbi request reprint Tetraplex binding molecules as anti-cancer agents
    Kevin M Doherty
    Laboratory of Molecular Gerontology, National Institute on Aging, NIH, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Recent Pat Anticancer Drug Discov 1:185-200. 2006
    ..In this review, we summarize the current research developments and associated patents that bear relevance to understanding the mechanism and clinical application of tetraplex binding molecules as anti-cancer agents...
  33. pmc Delineation of WRN helicase function with EXO1 in the replicational stress response
    Monika Aggarwal
    National Institute on Aging, NIH, NIH Biomedical Research Center, Baltimore, MD 21224, USA
    DNA Repair (Amst) 9:765-76. 2010
    ..In contrast to WRN, BLM helicase defective in Bloom's syndrome failed to rescue rad50 MMS sensitivity, but partially restored IR resistance, suggesting a delineation of function by the human RecQ helicases...
  34. pmc Helicases as prospective targets for anti-cancer therapy
    Rigu Gupta
    Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Anticancer Agents Med Chem 8:390-401. 2008
    ....
  35. ncbi request reprint Werner protein is a target of DNA-dependent protein kinase in vivo and in vitro, and its catalytic activities are regulated by phosphorylation
    Parimal Karmakar
    Laboratory of Molecular Gerontology, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 277:18291-302. 2002
    ..Thus, WRN protein is a target for DNA-PK phosphorylation in vitro and in vivo, and this phosphorylation may be a way of regulating its different catalytic activities, possibly in the repair of DNA dsb...
  36. ncbi request reprint Colocalization, physical, and functional interaction between Werner and Bloom syndrome proteins
    Cayetano Von Kobbe
    Laboratory of Molecular Gerontology, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 277:22035-44. 2002
    ..Our observation that RecQ family members interact provides new insights into the complex phenotypic manifestations resulting from the loss of these proteins...
  37. ncbi request reprint The processing of Holliday junctions by BLM and WRN helicases is regulated by p53
    Qin Yang
    Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 277:31980-7. 2002
    ..Our results are consistent with a novel mechanism for p53-mediated regulation of DNA recombinational repair that involves p53 post-translational modifications and functional protein-protein interactions with BLM and WRN DNA helicases...
  38. pmc FANCJ helicase operates in the Fanconi Anemia DNA repair pathway and the response to replicational stress
    Yuliang Wu
    Laboratory of Molecular Gerontology, National Institute on Aging, NIH, NIH Biomedical Research Center, Baltimore, MD 21224, USA
    Curr Mol Med 9:470-82. 2009
    ..The molecular roles of FANCJ in DNA repair and the response to replicational stress will be discussed...
  39. ncbi request reprint The transcriptional response after oxidative stress is defective in Cockayne syndrome group B cells
    Kasper J Kyng
    Laboratory of Molecular Gerontology, National Institute on Aging, 5600 Nathan Schock Drive, National Institute of Health, Baltimore, MD 21224, USA
    Oncogene 22:1135-49. 2003
    ..Some major defects are in the transcription of genes involved in DNA repair, signal transduction, and ribosomal functions...
  40. pmc Distinct roles of RECQ1 in the maintenance of genomic stability
    Yuliang Wu
    Laboratory of Molecular Gerontology, National Institute on Aging, NIH, NIH Biomedical Research Center, 251 Bayview Blvd, Baltimore, MD 21224, USA
    DNA Repair (Amst) 9:315-24. 2010
    ..In this review, we will summarize our current knowledge of RECQ1 roles in cellular nucleic acid metabolism and propose avenues of investigation for future studies...
  41. pmc FANCJ uses its motor ATPase to destabilize protein-DNA complexes, unwind triplexes, and inhibit RAD51 strand exchange
    Joshua A Sommers
    Laboratory of Molecular Gerontology, NIA, National Institutes of Health, National Institutes of Health Biomedical Research Center, Baltimore, Maryland 21224, USA
    J Biol Chem 284:7505-17. 2009
    ..Consistent with this, we show that FANCJ can inhibit RAD51 strand exchange, an activity that is likely to be important for its role in controlling DNA repair through homologous recombination...
  42. pmc Fanconi anemia group J mutation abolishes its DNA repair function by uncoupling DNA translocation from helicase activity or disruption of protein-DNA complexes
    Yuliang Wu
    Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, National Institutes of Health Biomedical Research Center, Baltimore, MD 21224, USA
    Blood 116:3780-91. 2010
    ..The ability of FANCJ to use the energy from ATP hydrolysis to produce the force required to unwind DNA or destabilize protein bound to DNA is required for its role in DNA repair...
  43. pmc FANCJ (BACH1) helicase forms DNA damage inducible foci with replication protein A and interacts physically and functionally with the single-stranded DNA-binding protein
    Rigu Gupta
    Laboratory of Molecular Gerontology, National Institute on Aging NIA, National Institutes of Health NIH, Baltimore, MD 21224, USA
    Blood 110:2390-8. 2007
    ..These findings identify RPA as the first regulatory partner of FANCJ. The FANCJ-RPA interaction is likely to be important for the role of the helicase to more efficiently unwind DNA repair intermediates to maintain genomic stability...
  44. pmc FANCJ helicase uniquely senses oxidative base damage in either strand of duplex DNA and is stimulated by replication protein A to unwind the damaged DNA substrate in a strand-specific manner
    Avvaru N Suhasini
    Laboratory of Molecular Gerontology, Biomedical Research Center, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 284:18458-70. 2009
    ..These studies are relevant to the roles of RPA, FANCJ, and other DNA helicases in the metabolism of damaged DNA that can interfere with basic cellular processes of DNA metabolism...
  45. ncbi request reprint Inhibition of Werner syndrome helicase activity by benzo[c]phenanthrene diol epoxide dA adducts in DNA is both strand-and stereoisomer-dependent
    Henry C Driscoll
    Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, DHHS, Baltimore, Maryland 21224 6825, USA
    J Biol Chem 278:41126-35. 2003
    ..Thus, the unwinding activity of RecQ helicases is sensitive to the strand, orientation, and stereochemistry of intercalated polycyclic aromatic hydrocarbon adducts...
  46. pmc Unique and important consequences of RECQ1 deficiency in mammalian cells
    Sudha Sharma
    Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, Department of Health and Human Services, Baltimore, Maryland, USA
    Cell Cycle 7:989-1000. 2008
    ..Collectively, these studies provide the first evidence for an important role of RECQ1 to confer chromosomal stability that is unique from that of other RecQ helicases and suggest its potential involvement in tumorigenesis...
  47. ncbi request reprint Analysis of the DNA substrate specificity of the human BACH1 helicase associated with breast cancer
    Rigu Gupta
    Laboratory of Molecular Gerontology, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 280:25450-60. 2005
    ....
  48. ncbi request reprint Biochemical assays for the characterization of DNA helicases
    Robert M Brosh
    Laboratory of Molecular Gerontology, National Institute on Aging, NIH, Baltimore, MD, USA
    Methods Mol Biol 314:397-415. 2006
    ....
  49. ncbi request reprint Enzymatic mechanism of the WRN helicase/nuclease
    Robert M Brosh
    Laboratory of Molecular Gerontology, National Institute of Aging IRP, National Institutes of Health, Baltimore, Maryland, USA
    Methods Enzymol 409:52-85. 2006
    ....
  50. ncbi request reprint Roles of the Werner syndrome protein in pathways required for maintenance of genome stability
    Robert M Brosh
    Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Exp Gerontol 37:491-506. 2002
    ..These interactions are being discussed as they shed light on the molecular pathways in which Werner protein participates. Insight into these pathways brings insight into the aging process...
  51. pmc Differential requirement for the ATPase domain of the Cockayne syndrome group B gene in the processing of UV-induced DNA damage and 8-oxoguanine lesions in human cells
    Rebecca R Selzer
    Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224 6825, USA
    Nucleic Acids Res 30:782-93. 2002
    ..The transfection of the mutant or wild-type CSB gene into the CS1AN.S3.G2 cells did not alter the expression of the subset of genes examined by cDNA array analysis...
  52. pmc The interaction site of Flap Endonuclease-1 with WRN helicase suggests a coordination of WRN and PCNA
    Sudha Sharma
    Laboratory of Molecular Gerontology, National Institute on Aging, NIH, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Nucleic Acids Res 33:6769-81. 2005
    ..These studies provide new insights to the interaction of WRN and BLM helicases with FEN-1, and how these interactions might be regulated with the PCNA-FEN-1 interaction during DNA replication and repair...
  53. pmc Inhibition of BACH1 (FANCJ) helicase by backbone discontinuity is overcome by increased motor ATPase or length of loading strand
    Rigu Gupta
    Laboratory of Molecular Gerontology, National Institute on Aging, NIH, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Nucleic Acids Res 34:6673-83. 2006
    ..Alternatively, increasing the length of the 5' tail of the DNA substrate allowed BACH1 to overcome the backbone discontinuity, suggesting that BACH1 loading mechanism is critical for its ability to unwind damaged DNA molecules...
  54. ncbi request reprint Phenotypic consequences of mutations in the conserved motifs of the putative helicase domain of the human Cockayne syndrome group B gene
    Meltem Muftuoglu
    Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Gene 283:27-40. 2002
    ....
  55. pmc WRN helicase defective in the premature aging disorder Werner syndrome genetically interacts with topoisomerase 3 and restores the top3 slow growth phenotype of sgs1 top3
    Monika Aggarwal
    Laboratory of Molecular Gerontology, National Institute on Aging, NIH, NIH, NIH Biomedical Research Center, 251 Bayview Blvd, Suite 100, Rm 06B125, Baltimore, MD 21224, USA
    Aging (Albany NY) 1:219-33. 2009
    ..Proposed roles of WRN in genetic pathways important for the suppression of genomic instability are discussed...
  56. pmc Molecular analyses of DNA helicases involved in the replicational stress response
    Yuliang Wu
    Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, NIH Biomedical Research Center, 251 Bayview Blvd, Baltimore, MD 21224, USA
    Methods 51:303-12. 2010
    ..The procedures to study these helicase functions are described in step-by-step detail to enable researchers interested in nucleic acid metabolism and related fields to apply these techniques to their own research questions...
  57. pmc Cockayne syndrome group B protein has novel strand annealing and exchange activities
    Meltem Muftuoglu
    Laboratory of Molecular Gerontology, National Institute on Aging, NIH, Baltimore, MD 21224, USA
    Nucleic Acids Res 34:295-304. 2006
    ..Potential roles of CSB in cooperation with strand annealing and exchange activities for TCR and homologous recombination are discussed...
  58. pmc Mechanisms of RecQ helicases in pathways of DNA metabolism and maintenance of genomic stability
    Sudha Sharma
    Laboratory of Molecular Gerontology, National Institute on Aging, NIH, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Biochem J 398:319-37. 2006
    ....
  59. pmc Human replication protein A melts a DNA triple helix structure in a potent and specific manner
    Yuliang Wu
    Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, Maryland 21224, USA
    Biochemistry 47:5068-77. 2008
    ..On the basis of our results, we suggest that the abundance of RPA known to exist in vivo is likely to be a strong deterrent to the stability of triplexes that can potentially form from human genomic DNA sequences...
  60. pmc G-quadruplex nucleic acids and human disease
    Yuliang Wu
    Laboratory of Molecular Gerontology, National Institute on Aging, Baltimore, MD, USA
    FEBS J 277:3470-88. 2010
    ..In this minireview, we discuss the connections of G-quadruplex nucleic acids to human genetic diseases and cancer based on the recent literature...
  61. pmc Functional consequences of mutations in the conserved SF2 motifs and post-translational phosphorylation of the CSB protein
    Mette Christiansen
    Danish Center for Molecular Gerontology, University of Aarhus, DK 8000 Aarhus C, Denmark
    Nucleic Acids Res 31:963-73. 2003
    ..These observations may have significant implications for the function of CSB in vivo...
  62. pmc RECQ1 possesses DNA branch migration activity
    Dmitry V Bugreev
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102 1192, USA
    J Biol Chem 283:20231-42. 2008
    ..The newly found enzymatic properties of the RECQ1 helicase may have important implications for the function of RECQ1 in maintenance of genomic stability...
  63. pmc BRCA-FA pathway as a target for anti-tumor drugs
    Rachel Litman
    Department of Cancer Biology, University of Massachusetts Medical School, 364 Plantation St, Worcester, MA 01605, USA
    Anticancer Agents Med Chem 8:426-30. 2008
    ..In the future, identifying patients with susceptible tumors and discovering additional DNA repair targets amenable to anti-tumor drugs will have a major impact on the course of cancer treatment...
  64. pmc Analysis of the unwinding activity of the dimeric RECQ1 helicase in the presence of human replication protein A
    Sheng Cui
    International Centre for Genetic Engineering and Biotechnology, Padriciano 99, I 34012 Trieste, Italy
    Nucleic Acids Res 32:2158-70. 2004
    ..In addition, our far western, ELISA and co-immunoprecipitation experiments demonstrate that RECQ1 physically interacts with the 70 kDa subunit of hRPA and that this interaction is not mediated by DNA...
  65. pmc The FANCJ/MutLalpha interaction is required for correction of the cross-link response in FA-J cells
    Min Peng
    Department of Cancer Biology, University of Massachusetts Medical School Women s Cancers Program, UMASS Memorial Cancer Center, Worcester, MA, USA
    EMBO J 26:3238-49. 2007
    ..The functional role of the FANCJ/MutLalpha complex demonstrates a novel link between FA and MMR, and predicts a broader role for FANCJ in DNA damage signaling independent of BRCA1...
  66. ncbi request reprint DNA repair as a target for anti-cancer therapy
    Robert M Brosh
    Anticancer Agents Med Chem 8:350. 2008
  67. pmc FANCJ helicase defective in Fanconia anemia and breast cancer unwinds G-quadruplex DNA to defend genomic stability
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