P J Blackshear

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc The tandem CCCH zinc finger protein tristetraprolin and its relevance to cytokine mRNA turnover and arthritis
    Danielle M Carrick
    Office of Clinical Research, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA
    Arthritis Res Ther 6:248-64. 2004
  2. pmc Identification of the anti-inflammatory protein tristetraprolin as a hyperphosphorylated protein by mass spectrometry and site-directed mutagenesis
    Heping Cao
    Laboratories of Neurobiology and Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    Biochem J 394:285-97. 2006
  3. pmc A unique C-terminal repeat domain maintains the cytosolic localization of the placenta-specific tristetraprolin family member ZFP36L3
    Elizabeth D Frederick
    NIEHS, NIH, Research Triangle Park, NC 27709, USA
    J Biol Chem 283:14792-800. 2008
  4. pmc Phosphorylation of human tristetraprolin in response to its interaction with the Cbl interacting protein CIN85
    Vishram P Kedar
    The Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, United States of America
    PLoS ONE 5:e9588. 2010
  5. ncbi request reprint Characteristics of the interaction of a synthetic human tristetraprolin tandem zinc finger peptide with AU-rich element-containing RNA substrates
    Perry J Blackshear
    Laboratory of Signal Transduction, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 278:19947-55. 2003
  6. ncbi request reprint Zfp36l3, a rodent X chromosome gene encoding a placenta-specific member of the Tristetraprolin family of CCCH tandem zinc finger proteins
    Perry J Blackshear
    Laboratory of Neurobiology, Office of Clinical Research, National Institute of Environmental Health Sciences, North Carolina 27709, USA
    Biol Reprod 73:297-307. 2005
  7. ncbi request reprint The NIEHS Xenopus maternal EST project: interim analysis of the first 13,879 ESTs from unfertilized eggs
    P J Blackshear
    Office of Clinical Research and Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, 111 Alexander Drive, Research Triangle Park, NC 27709, USA
    Gene 267:71-87. 2001
  8. ncbi request reprint Disruption of the gene encoding the mitogen-regulated translational modulator PHAS-I in mice
    P J Blackshear
    Department of Medicine, The Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 272:31510-4. 1997
  9. ncbi request reprint Tristetraprolin and other CCCH tandem zinc-finger proteins in the regulation of mRNA turnover
    P J Blackshear
    Office of Clinical Research and the Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Biochem Soc Trans 30:945-52. 2002
  10. ncbi request reprint Transgenic complementation of MARCKS deficiency with a nonmyristoylatable, pseudo-phosphorylated form of MARCKS: evidence for simultaneous positive and dominant-negative effects on central nervous system development
    H S Kim
    Office of Clinical Research and Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, 27709, USA
    Dev Biol 200:146-57. 1998

Collaborators

Detail Information

Publications88

  1. pmc The tandem CCCH zinc finger protein tristetraprolin and its relevance to cytokine mRNA turnover and arthritis
    Danielle M Carrick
    Office of Clinical Research, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA
    Arthritis Res Ther 6:248-64. 2004
    ..The TTP pathway of TNF-alpha and GM-CSF mRNA degradation is a possible novel target for anti-TNF-alpha therapies for rheumatoid arthritis, and also for other conditions proven to respond to anti-TNF-alpha therapy...
  2. pmc Identification of the anti-inflammatory protein tristetraprolin as a hyperphosphorylated protein by mass spectrometry and site-directed mutagenesis
    Heping Cao
    Laboratories of Neurobiology and Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    Biochem J 394:285-97. 2006
    ....
  3. pmc A unique C-terminal repeat domain maintains the cytosolic localization of the placenta-specific tristetraprolin family member ZFP36L3
    Elizabeth D Frederick
    NIEHS, NIH, Research Triangle Park, NC 27709, USA
    J Biol Chem 283:14792-800. 2008
    ..The significance of this difference in subcellular localization to the physiology of placental trophoblast cells, where ZFP36L3 is selectively expressed, remains to be determined...
  4. pmc Phosphorylation of human tristetraprolin in response to its interaction with the Cbl interacting protein CIN85
    Vishram P Kedar
    The Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, United States of America
    PLoS ONE 5:e9588. 2010
    ..TTP plays a critical role in the decay of tumor necrosis factor alpha (TNF) mRNA, among others, by binding AU-rich RNA elements in the 3'-untranslated regions of this transcript and promoting its deadenylation and degradation...
  5. ncbi request reprint Characteristics of the interaction of a synthetic human tristetraprolin tandem zinc finger peptide with AU-rich element-containing RNA substrates
    Perry J Blackshear
    Laboratory of Signal Transduction, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 278:19947-55. 2003
    ..These data suggest that this TTP peptide-RNA complex is structured in solution and might be amenable to NMR structure determination...
  6. ncbi request reprint Zfp36l3, a rodent X chromosome gene encoding a placenta-specific member of the Tristetraprolin family of CCCH tandem zinc finger proteins
    Perry J Blackshear
    Laboratory of Neurobiology, Office of Clinical Research, National Institute of Environmental Health Sciences, North Carolina 27709, USA
    Biol Reprod 73:297-307. 2005
    ..Its presumed role in placental physiology may be unique to rodents or murine rodents, but this role may be subsumed by other family members in nonrodents...
  7. ncbi request reprint The NIEHS Xenopus maternal EST project: interim analysis of the first 13,879 ESTs from unfertilized eggs
    P J Blackshear
    Office of Clinical Research and Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, 111 Alexander Drive, Research Triangle Park, NC 27709, USA
    Gene 267:71-87. 2001
    ....
  8. ncbi request reprint Disruption of the gene encoding the mitogen-regulated translational modulator PHAS-I in mice
    P J Blackshear
    Department of Medicine, The Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 272:31510-4. 1997
    ....
  9. ncbi request reprint Tristetraprolin and other CCCH tandem zinc-finger proteins in the regulation of mRNA turnover
    P J Blackshear
    Office of Clinical Research and the Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Biochem Soc Trans 30:945-52. 2002
    ..Together, the TTP family members should be capable of complex regulation of short-lived mRNAs containing this type of AU-rich instability motif...
  10. ncbi request reprint Transgenic complementation of MARCKS deficiency with a nonmyristoylatable, pseudo-phosphorylated form of MARCKS: evidence for simultaneous positive and dominant-negative effects on central nervous system development
    H S Kim
    Office of Clinical Research and Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, 27709, USA
    Dev Biol 200:146-57. 1998
    ....
  11. ncbi request reprint Identification of four CCCH zinc finger proteins in Xenopus, including a novel vertebrate protein with four zinc fingers and severely restricted expression
    J De
    Office of Clinical Research and Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Gene 228:133-45. 1999
    ..Maternal expression was also seen with the other three messages, suggesting the possibility that these proteins are involved in regulating mRNA stability in oocyte maturation and/or early embryogenesis...
  12. ncbi request reprint Interactions of CCCH zinc finger proteins with mRNA. Binding of tristetraprolin-related zinc finger proteins to Au-rich elements and destabilization of mRNA
    W S Lai
    Office of Clinical Research and Laboratory of Signal Transduction, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 275:17827-37. 2000
    ....
  13. ncbi request reprint Promoter sequence, expression, and fine chromosomal mapping of the human gene (MLP) encoding the MARCKS-like protein: identification of neighboring and linked polymorphic loci for MLP and MACS and use in the evaluation of human neural tube defects
    D J Stumpo
    Office of Clinical Research, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Genomics 49:253-64. 1998
    ..Nonetheless, the identification of linked and neighboring polymorphisms for MACS and MLP should permit similar genetic studies in other groups of patients with neural tube defects and other neurodevelopmental abnormalities...
  14. ncbi request reprint Phosphorylation of tristetraprolin, a potential zinc finger transcription factor, by mitogen stimulation in intact cells and by mitogen-activated protein kinase in vitro
    G A Taylor
    Howard Hughes Medical Institute Laboratories, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 270:13341-7. 1995
    ..These results suggest that TTP may be phosphorylated by MAP kinases in vivo and that this phosphorylation may regulate its function...
  15. pmc Evidence that tristetraprolin binds to AU-rich elements and promotes the deadenylation and destabilization of tumor necrosis factor alpha mRNA
    W S Lai
    Office of Clinical Research and Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Mol Cell Biol 19:4311-23. 1999
    ..This CCCH protein is likely to be important in the deadenylation and degradation of TNF-alpha mRNA and perhaps other ARE-containing mRNAs, both in normal physiology and in certain pathological conditions...
  16. ncbi request reprint Roles of tumor necrosis factor-alpha receptor subtypes in the pathogenesis of the tristetraprolin-deficiency syndrome
    E Carballo
    Office of Clinical Research and Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Blood 98:2389-95. 2001
    ..Finally, the results showed that TNFR1 plays an important role in mediating TNF-alpha-induced changes in TNF-alpha and GM-CSF mRNA stability...
  17. ncbi request reprint Feedback inhibition of macrophage tumor necrosis factor-alpha production by tristetraprolin
    E Carballo
    Office of Clinical Research and Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Science 281:1001-5. 1998
    ..These findings identify TTP as a component of a negative feedback loop that interferes with TNF-alpha production by destabilizing its messenger RNA. This pathway represents a potential target for anti-TNF-alpha therapies...
  18. ncbi request reprint Molecular cloning, sequence, and expression of a cDNA encoding the chicken myristoylated alanine-rich C kinase substrate (MARCKS)
    J M Graff
    Howard Hughes Medical Institute Laboratories, Durham, North Carolina 27710
    Mol Endocrinol 3:1903-6. 1989
    ..These conserved regions are likely to represent domains of some functional importance for this widely distributed cellular substrate for PKC...
  19. ncbi request reprint Developmental expression of MARCKS and protein kinase C in mice in relation to the exencephaly resulting from MARCKS deficiency
    P J Blackshear
    Howard Hughes Medical Institute Laboratories, Duke University Medical Center, Durham, NC 27710, USA
    Brain Res Dev Brain Res 96:62-75. 1996
    ....
  20. ncbi request reprint Myristoylation-dependent and electrostatic interactions exert independent effects on the membrane association of the myristoylated alanine-rich protein kinase C substrate protein in intact cells
    S L Swierczynski
    Howard Hughes Medical Institute, Division of Endocrinology, Metabolism, and Nutrition, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 271:23424-30. 1996
    ..These results indicate that myristoylation and electrostatic interactions involving the PSD exert independent, essentially additive effects on the membrane association of MARCKS in intact cells...
  21. ncbi request reprint Evidence that tristetraprolin is a physiological regulator of granulocyte-macrophage colony-stimulating factor messenger RNA deadenylation and stability
    E Carballo
    Office of Clinical Research and Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Blood 95:1891-9. 2000
    ..TTP appears to be a physiological regulator of GM-CSF mRNA deadenylation and stability. (Blood. 2000;95:1891-1899)..
  22. ncbi request reprint Widespread neuronal ectopia associated with secondary defects in cerebrocortical chondroitin sulfate proteoglycans and basal lamina in MARCKS-deficient mice
    P J Blackshear
    Department of Medicine, and The Sarah W Stedman Center for Nutritional Studies, Duke University Medical Center, Durham, North Carolina 27710, USA
    Exp Neurol 145:46-61. 1997
    ....
  23. ncbi request reprint Mechanisms of MARCKS gene activation during Xenopus development
    Y Shi
    Howard Hughes Medical Institute and the Section of Diabetes and Metabolism, Division of Endocrinology, Metabolism and Nutrition, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 272:29290-300. 1997
    ..These studies not only increase our knowledge of the transcriptional regulation of the MARCKS genes but also have implications for the mechanisms responsible for zygotic activation of the Xenopus genome at MBT...
  24. pmc The human TTP protein: sequence, alignment with related proteins, and chromosomal localization of the mouse and human genes
    G A Taylor
    Howard Hughes Medical Institute Laboratories, Duke University Medical Center, Durham, NC 27710
    Nucleic Acids Res 19:3454. 1991
  25. ncbi request reprint Protein kinase C-mediated phosphorylation and calmodulin binding of recombinant myristoylated alanine-rich C kinase substrate (MARCKS) and MARCKS-related protein
    G M Verghese
    Howard Hughes Medical Institute Laboratories, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710
    J Biol Chem 269:9361-7. 1994
    ..These studies suggest that intact, recombinant MARCKS and MRP are accurately modeled by their synthetic PSCBD peptides with respect to PKC phosphorylation kinetics and their phosphorylation-dependent calmodulin binding properties...
  26. ncbi request reprint Rapid insulin-stimulated accumulation of an mRNA encoding a proline-rich protein
    W S Lai
    Howard Hughes Medical Institute Laboratories, Durham, North Carolina 27710
    J Biol Chem 265:16556-63. 1990
    ..A single TTP gene appeared to be present in the mouse genome. This gene joins the group of genes whose members are rapidly transcribed in response to insulin and other mitogens...
  27. ncbi request reprint Interactions of CCCH zinc finger proteins with mRNA: tristetraprolin-mediated AU-rich element-dependent mRNA degradation can occur in the absence of a poly(A) tail
    W S Lai
    Office of Clinical Research and Laboratory of Signal Transduction, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 276:23144-54. 2001
    ....
  28. ncbi request reprint Characteristics of the intron involvement in the mitogen-induced expression of Zfp-36
    W S Lai
    Howard Hughes Medical Institute Laboratories, Section of Diabetes and Metabolism, Division of Endocrinology, Metabolism and Nutrition and the Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 273:506-17. 1998
    ....
  29. pmc Decreased sensitivity of tristetraprolin-deficient cells to p38 inhibitors suggests the involvement of tristetraprolin in the p38 signaling pathway
    E Carballo
    Office of Clinical Research and Laboratory of Signal Transduction, NIEHS, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 276:42580-7. 2001
    ..These results suggest that TTP may be a component of the signaling cascade, initiated by inflammatory stimuli and mediated in part by activation of p38, that ultimately leads to enhanced secretion of tumor necrosis factor alpha...
  30. ncbi request reprint A pathogenetic role for TNF alpha in the syndrome of cachexia, arthritis, and autoimmunity resulting from tristetraprolin (TTP) deficiency
    G A Taylor
    Howard Hughes Medical Institute Laboratory, Duke University Medical Center, Durham, North Carolina 27710, USA
    Immunity 4:445-54. 1996
    ..TTP-deficient mice may serve as useful models of the autoimmune inflammatory state resulting from chronic effective TNF alpha excess...
  31. ncbi request reprint Cloning and characterization of RECQL, a potential human homologue of the Escherichia coli DNA helicase RecQ
    K L Puranam
    Howard Hughes Medical Institute Laboratories, Duke University Medical Center, Durham, North Carolina 27710
    J Biol Chem 269:29838-45. 1994
    ..Based on its sequence similarity to the E. coli RecQ protein, it is possible that the putative human helicase RECQL may play a role in the repair of DNA that is damaged by ultraviolet light or other mutagens...
  32. ncbi request reprint Characterization of the phosphorylation sites in the chicken and bovine myristoylated alanine-rich C kinase substrate protein, a prominent cellular substrate for protein kinase C
    J M Graff
    Howard Hughes Medical Institute Laboratories, Durham, North Carolina 27710
    J Biol Chem 264:11912-9. 1989
    ....
  33. ncbi request reprint The human myristoylated alanine-rich C kinase substrate (MARCKS) gene (MACS). Analysis of its gene product, promoter, and chromosomal localization
    D M Harlan
    Howard Hughes Medical Institute Laboratories, Duke University Medical Center, Durham, North Carolina 27710
    J Biol Chem 266:14399-405. 1991
    ..Using an insert from the human genomic clone as a probe, we identified human chromosome 6, q21-qter, as the location of the MARCKS gene; this has been assigned the gene symbol MACS...
  34. ncbi request reprint Nonmyristoylated MARCKS complements some but not all of the developmental defects associated with MARCKS deficiency in mice
    S L Swierczynski
    Howard Hughes Medical Institute, Durham, North Carolina 27710, USA
    Dev Biol 179:135-47. 1996
    ..These results indicate that myristoylation of MARCKS is not required for the protein to correct many of the developmental abnormalities characteristic of its deficiency...
  35. ncbi request reprint Overexpression of the myristoylated alanine-rich C-kinase substrate inhibits cell adhesion to extracellular matrix components
    G Spizz
    Office of Clinical Research and Laboratory of Signal Transduction, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina, 27709, USA
    J Biol Chem 276:32264-73. 2001
    ..These data suggest a possible myristoylation-dependent function of MARCKS to inhibit cellular adhesion to extracellular matrix proteins, indicating a potential mechanism for the cell migration defects seen in the MARCKS-deficient mice...
  36. ncbi request reprint Promoter analysis of Zfp-36, the mitogen-inducible gene encoding the zinc finger protein tristetraprolin
    W S Lai
    Howard Hughes Medical Institute Laboratories, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 270:25266-72. 1995
    ..These results suggest that the response of Zfp-36 to serum and other mitogens is mediated by a series of cis-acting elements acting in concert to confer full inducible transcription of this gene...
  37. ncbi request reprint Characteristics of the F52 protein, a MARCKS homologue
    P J Blackshear
    Howard Hughes Medical Institute Laboratories, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710
    J Biol Chem 267:13540-6. 1992
    ..The F52 protein is clearly a member of the MARCKS family as defined by primary sequence; in addition, the two proteins share several key attributes that may be functionally important...
  38. ncbi request reprint The MARCKS family of cellular protein kinase C substrates
    P J Blackshear
    Howard Hughes Medical Institute Laboratories, Duke University Medical Center, Durham, North Carolina 27710
    J Biol Chem 268:1501-4. 1993
  39. ncbi request reprint Members of the tristetraprolin family of tandem CCCH zinc finger proteins exhibit CRM1-dependent nucleocytoplasmic shuttling
    Ruth S Phillips
    Department of Pharmacology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 277:11606-13. 2002
    ..These findings suggest possible roles for nuclear import and export in the regulation of cellular TTP, CMG1, and TIS11D activity...
  40. ncbi request reprint Identification and characterization of cathepsin B as the cellular MARCKS cleaving enzyme
    G Spizz
    Howard Hughes Medical Institute, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 272:23833-42. 1997
    ..The data also suggest a direct interaction between MARCKS and cathepsin B in cells and leave open the possibility that MARCKS may in some way regulate the protease for which it is a substrate...
  41. pmc Chorioallantoic fusion defects and embryonic lethality resulting from disruption of Zfp36L1, a gene encoding a CCCH tandem zinc finger protein of the Tristetraprolin family
    Deborah J Stumpo
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Mol Cell Biol 24:6445-55. 2004
    ..We postulate that lack of Zfp36L1 expression during mid-gestation results in the abnormal stabilization of one or more mRNAs whose encoded proteins lead directly or indirectly to abnormal placentation and fetal death...
  42. ncbi request reprint Early biochemical events in insulin-stimulated fluid phase endocytosis
    D M Pitterle
    Departments of Medicine and Biochemistry, Duke University Medical Center, Durham, North Carolina, 27710, USA
    Am J Physiol 276:E94-E105. 1999
    ..The results indicate that the insulin receptor, IRS-1, Ras, and, perhaps in certain cell types, PI 3'-kinase are involved in mediating insulin-stimulated FPE...
  43. pmc Immunological characterization of tristetraprolin as a low abundance, inducible, stable cytosolic protein
    Heping Cao
    Laboratory of Signal Transduction and Office of Clinical Research, National Institute of Environmental Health Sciences, NIH DHHS, 111 Alexander Drive, Research Triangle Park, NC 27709, USA
    J Biol Chem 279:21489-99. 2004
    ..TTP appears to be a low abundance, cytosolic protein in unstimulated cells and tissues, but once induced is relatively stable, in contrast to its very labile mRNA...
  44. ncbi request reprint Polymorphisms in the genes encoding members of the tristetraprolin family of human tandem CCCH zinc finger proteins
    Perry J Blackshear
    Office of Clinical Research and Laboratory of Signal Transduction, A2 05 National Institute of Environmental Health Sciences, 111 Alexander Drive, Research Triangle Park, NC 27709, USA
    Prog Nucleic Acid Res Mol Biol 75:43-68. 2003
    ..The functional significance of this and the other polymorphisms identified remains to be determined by both biochemical and population linkage studies...
  45. ncbi request reprint Interactions of CCCH zinc finger proteins with mRNA: non-binding tristetraprolin mutants exert an inhibitory effect on degradation of AU-rich element-containing mRNAs
    Wi S Lai
    Office of Clinical Research and the Laboratory of Signal Transduction, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 277:9606-13. 2002
    ..These data suggest that TTP may act, at least in part, by physically interacting with an enzyme activity or protein complex and functionally stimulating its ability to deadenylate class II ARE-containing mRNAs...
  46. ncbi request reprint Genetic variations in ZFP36 and their possible relationship to autoimmune diseases
    Danielle Mercatante Carrick
    Office of Clinical Research, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    J Autoimmun 26:182-96. 2006
    ..The data presented here suggest a tentative association between ZFP36 and RA. This finding, as well as the ZFP36 polymorphisms and haplotypes identified here, should form the basis for future association studies in autoimmune diseases...
  47. ncbi request reprint Chromosomal localization of the gene encoding the human DNA helicase RECQL and its mouse homologue
    K L Puranam
    Howard Hughes Medical Institute Laboratories, Department of Medicine and Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    Genomics 26:595-8. 1995
    ..The corresponding mouse gene, Recql, was mapped to the telomeric end of mouse chromosome 6 by analysis of DNA from an interspecific cross...
  48. doi request reprint The Environmental Polymorphisms Registry: a DNA resource to study genetic susceptibility loci
    Patricia C Chulada
    Clinical Research Program, National Institute of Environmental Health Sciences, 111 T W Alexander Drive, Research Triangle Park, NC 27709, USA
    Hum Genet 123:207-14. 2008
    ..The success of the EPR based on the number of subjects recruited and genetic studies underway, suggests that it will be a model for future DNA resources...
  49. pmc Tristetraprolin and its family members can promote the cell-free deadenylation of AU-rich element-containing mRNAs by poly(A) ribonuclease
    Wi S Lai
    Laboratory of Signal Transduction Office of Clinical Research, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    Mol Cell Biol 23:3798-812. 2003
    ..This interaction may be responsible for the ability of TTP and its family members to promote the deadenylation of such transcripts in intact cells...
  50. pmc Expression and purification of recombinant tristetraprolin that can bind to tumor necrosis factor-alpha mRNA and serve as a substrate for mitogen-activated protein kinases
    Heping Cao
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Mail Drop F3 04, Research Triangle Park, NC 27709, USA
    Arch Biochem Biophys 412:106-20. 2003
    ....
  51. ncbi request reprint Immunogenetic risk and protective factors for the idiopathic inflammatory myopathies: distinct HLA-A, -B, -Cw, -DRB1, and -DQA1 allelic profiles distinguish European American patients with different myositis autoantibodies
    Terrance P O'Hanlon
    National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892, USA
    Medicine (Baltimore) 85:111-27. 2006
    ....
  52. pmc Diversity in penaeidin antimicrobial peptide form and function
    Brandon J Cuthbertson
    Laboratory of Signal Transduction, NIH NIEHS, P O Box 12233 MD F3 04, 111 TW Alexander Drive, Research Triangle Park, NC 27709 2233, USA
    Dev Comp Immunol 32:167-81. 2008
    ....
  53. pmc The feasibility of creating a population-based national twin registry in the United States
    Patricia C Chulada
    Program in Clinical Research, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA
    Twin Res Hum Genet 9:919-26. 2006
    ....
  54. ncbi request reprint Rat ornithine decarboxylase gene. Nucleotide sequence, potential regulatory elements, and comparison to the mouse gene
    L Wen
    Howard Hughes Medical Institute Laboratories, Durham, North Carolina 27710
    J Biol Chem 264:9016-21. 1989
    ..The potential regulatory elements identified in both the promoter region of the gene and the 5'-untranslated region of the mRNA may be involved in the complex regulation of rat ornithine decarboxylase expression...
  55. pmc Targeted disruption of Zfp36l2, encoding a CCCH tandem zinc finger RNA-binding protein, results in defective hematopoiesis
    Deborah J Stumpo
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Blood 114:2401-10. 2009
    ..These data establish Zfp36l2 as a critical modulator of definitive hematopoiesis and suggest a novel regulatory pathway involving control of mRNA stability in the life cycle of hematopoietic stem and progenitor cells...
  56. pmc Characterization of zfs1 as an mRNA-binding and -destabilizing protein in Schizosaccharomyces pombe
    Brandon J Cuthbertson
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, 111 T W Alexander Drive, Research Triangle Park, NC 27709, USA
    J Biol Chem 283:2586-94. 2008
    ..zfs1 and its targets in S. pombe represent a useful model system for studies of zinc finger protein/AU-rich element interactions that result in mRNA decay...
  57. pmc G-protein pathway suppressor 2 (GPS2) interacts with the regulatory factor X4 variant 3 (RFX4_v3) and functions as a transcriptional co-activator
    Donghui Zhang
    Laboratories of Respiratory Biology and Neurobiology, Office of Clinical Research, NIEHS, NIH, Research Triangle Park, NC 27709, USA
    J Biol Chem 283:8580-90. 2008
    ..Based on these data, we conclude that GPS2 interacts with RFX4_v3 to modulate transactivation of genes involved in brain morphogenesis, including Cx3Cl1...
  58. ncbi request reprint The CCCH tandem zinc-finger protein Zfp36l2 is crucial for female fertility and early embryonic development
    Silvia B V Ramos
    Laboratory of Signal Transduction, National Institute of Environmental Health Science, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    Development 131:4883-93. 2004
    ..This newly identified biological role for Zfp36l2 may have implications for maternal mRNA turnover in normal embryogenesis, and conceivably could be involved in some cases of unexplained human female infertility...
  59. ncbi request reprint Graded phenotypic response to partial and complete deficiency of a brain-specific transcript variant of the winged helix transcription factor RFX4
    Perry J Blackshear
    Office of Clinical Research, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Development 130:4539-52. 2003
    ..These findings may be relevant to human congenital hydrocephalus, a birth defect that affects approximately 0.6 per 1000 newborns...
  60. ncbi request reprint Chromosomal mapping of the human (MACS) and mouse (Macs) genes encoding the MARCKS protein
    P J Blackshear
    Howard Hughes Medical Institute, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710
    Genomics 14:168-74. 1992
    ....
  61. ncbi request reprint Load-dependent and -independent regulation of proinflammatory cytokine and cytokine receptor gene expression in the adult mammalian heart
    Georg Baumgarten
    Winters Center for Heart Failure Research, Department of Medicine, Houston VAMC and Baylor College of Medicine, Houston, Tex 77030, USA
    Circulation 105:2192-7. 2002
    ..Although previous studies have examined the effects of acute hemodynamic pressure overload on proinflammatory cytokine gene expression, the effects of sustained hemodynamic overloading have not been examined...
  62. pmc RECQL, a member of the RecQ family of DNA helicases, suppresses chromosomal instability
    Sudha Sharma
    NIEHS MD A2 05, 111 Alexander Drive, Research Triangle Park, NC 27709, USA
    Mol Cell Biol 27:1784-94. 2007
    ..Genetic background, functional redundancy, and perhaps other factors may protect the unstressed mouse from the types of abnormalities that might be expected from the severe chromosomal aberrations detected at the cellular level...
  63. ncbi request reprint Identification of potential target genes for RFX4_v3, a transcription factor critical for brain development
    Donghui Zhang
    Laboratory of Neurobiology, National Institute of Environmental Health Sciences, National Institutes of Health Research Triangle Park, North Carolina 27709, USA
    J Neurochem 98:860-75. 2006
    ..These data suggest that RFX4_v3 may act upstream of critical signaling pathways in the process of brain development...
  64. pmc Predictors of acquired lipodystrophy in juvenile-onset dermatomyositis and a gradient of severity
    April Bingham
    From Office of Clinical Research, National Institute of Environmental Health Sciences, National Institutes of Health, Bethesda, Maryland 20892 1301, USA
    Medicine (Baltimore) 87:70-86. 2008
    ..Further study is warranted to investigate the pathogenesis of acquired LD in patients with DM...
  65. pmc Left-sided cardiac valvulitis in tristetraprolin-deficient mice: the role of tumor necrosis factor alpha
    Sanjukta Ghosh
    Laboratory of Signal Transduction, Comparative Medicine Branch, National Institute of Environmental Health Sciences, 111 Alexander Drive, Research Triangle Park, NC 27709, USA
    Am J Pathol 176:1484-93. 2010
    ..These findings support the potential involvement of TNF and inflammation in the development of cardiac valve disease in man...
  66. pmc Regulatory factor X4 variant 3: a transcription factor involved in brain development and disease
    Donghui Zhang
    Laboratory of Neurobiology, National Institute of Environmental Health Sciences, National Institutes of Health, 111 Alexander Drive, Research Triangle Park, NC 27709, USA
    J Neurosci Res 85:3515-22. 2007
    ..This Mini-Review describes our current knowledge about RFX4_v3, an important protein that appears to be involved in many aspects of brain development and disease...
  67. ncbi request reprint Neuroanatomical development in the absence of PKC phosphorylation of the myristoylated alanine-rich C-kinase substrate (MARCKS) protein
    Cameron O Scarlett
    Office of Clinical Research and Laboratory of Signal Transduction, A2 05 National Institute of Environmental Health Sciences, 111 Alexander Drive, Research Triangle Park, NC 27709, USA
    Brain Res Dev Brain Res 144:25-42. 2003
    ..These data raise the possibility of a role for MARCKS in the netrin-regulated process of pontine nuclei formation...
  68. ncbi request reprint Comparative expression of tristetraprolin (TTP) family member transcripts in normal human tissues and cancer cell lines
    Danielle M Carrick
    The Office of Clinical Research, National Institute of Environmental Health Sciences, NIEHS MD A2 05, 111 Alexander Drive, Research Triangle Park, NC 27709, USA
    Arch Biochem Biophys 462:278-85. 2007
    ....
  69. pmc Inhibition of native and recombinant nicotinic acetylcholine receptors by the myristoylated alanine-rich C kinase substrate peptide
    Elaine A Gay
    Laboratory of Neurobiology, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    J Pharmacol Exp Ther 327:884-90. 2008
    ..The MARCKS ED peptide represents a novel antagonist of neuronal nAChRs that has considerable utility as a research tool...
  70. pmc Stimulation of polo-like kinase 3 mRNA decay by tristetraprolin
    Thierry J Horner
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Mol Cell Biol 29:1999-2010. 2009
    ..The regulation of Plk3 mRNA stability by TTP may influence the control of the cell cycle by this protein kinase...
  71. doi request reprint Chapter 4. Evaluating the control of mRNA decay in fission yeast
    Brandon J Cuthbertson
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA
    Methods Enzymol 449:73-95. 2008
    ..We hope to use this convenient experimental system to unravel the mechanism by which TTP family members, in this and other organisms, bind to mRNAs and promote their instability...
  72. ncbi request reprint Tristetraprolin down-regulates IL-2 gene expression through AU-rich element-mediated mRNA decay
    Rachel L Ogilvie
    Microbiology, Immunology and Cancer Biology Graduate Program, University of Minnesota, Minneapolis 55455, USA
    J Immunol 174:953-61. 2005
    ..Also, IL-2 mRNA was more stable in activated splenocytes from TTP knockout mice compared with wild-type mice. Taken together, these data suggest that TTP functions to down-regulate IL-2 gene expression through ARE-mediated mRNA decay...
  73. pmc Genome-wide analysis identifies interleukin-10 mRNA as target of tristetraprolin
    Georg Stoecklin
    Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 283:11689-99. 2008
    ..IL-10 mRNA levels were found to be elevated because of a reduced decay rate in primary macrophages from TTP(-/-) mice. Our study demonstrates the importance of experimental approaches for identifying targets of RNA-binding proteins...
  74. pmc Phosphorylation site analysis of the anti-inflammatory and mRNA-destabilizing protein tristetraprolin
    Heping Cao
    US Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Diet, Genomics, and Immunology Laboratory, 10300 Baltimore Avenue, Beltsville, MD 20705, USA
    Expert Rev Proteomics 4:711-26. 2007
    ..This information should provide the molecular basis for future investigation of TTP's regulatory functions in controlling proinflammatory cytokines...
  75. ncbi request reprint Interaction of retroviral Tax oncoproteins with tristetraprolin and regulation of tumor necrosis factor-alpha expression
    Jean Claude Twizere
    Biologie Cellulaire et Moleculaire, Faculté universitaire des Sciences agronomiques, Gembloux, Belgium
    J Natl Cancer Inst 95:1846-59. 2003
    ..e., primate T-cell leukemia viruses and bovine leukemia virus). To better understand the molecular pathways leading to cell transformation, we aimed to identify cellular proteins interacting with Tax...
  76. pmc Arthritis suppressor genes TIA-1 and TTP dampen the expression of tumor necrosis factor alpha, cyclooxygenase 2, and inflammatory arthritis
    Kristine Phillips
    Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, Smith 652, One Jimmy Fund Way, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 101:2011-6. 2004
    ..Our results suggest that TIA-1 and TTP are genetic modifiers of inflammatory arthritis that can alter the spectrum of cells that produce arthritogenic cytokines...
  77. pmc Mitogen-activated protein kinase-activated protein kinase 2 regulates tumor necrosis factor mRNA stability and translation mainly by altering tristetraprolin expression, stability, and binding to adenine/uridine-rich element
    Edward Hitti
    Medical School Hannover, Institute of Biochemistry, Carl Neuberg Str 1, D 30625 Hannover, Germany
    Mol Cell Biol 26:2399-407. 2006
    ..These data suggest that MK2 inhibits the mRNA destabilizing activity of TTP and, in parallel, codegradation of TTP together, with the target mRNA resulting in increased cellular levels of TTP...
  78. pmc Interferons limit inflammatory responses by induction of tristetraprolin
    Ines Sauer
    Max F Perutz Laboratories, Department of Microbiology and Immunobiology, University of Vienna, Dr Bohr Gasse 9, A 1030 Vienna, Austria
    Blood 107:4790-7. 2006
    ..Thus, our findings establish a link between interferon responses and TTP-mediated mRNA decay during inflammation, and propose a novel immunomodulatory role of IFNs...
  79. ncbi request reprint Myristoylated alanine-rich C kinase substrate-mediated neurotensin release via protein kinase C-delta downstream of the Rho/ROK pathway
    Jing Li
    Department of Surgery and Sealy Center for Cancer Cell Biology, The University of Texas Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 280:8351-7. 2005
    ..Collectively, these findings provide evidence for novel signaling pathways, including the sequential regulation of MARCKS activity by Rho/ROK and PKC-delta proteins, in stimulated gut peptide secretion...
  80. ncbi request reprint Cell type-specific upregulation of myristoylated alanine-rich C kinase substrate and protein kinase C-alpha, -beta I, -beta II, and -delta in microglia following kainic acid-induced seizures
    Su Yong Eun
    Department of Physiology, Cheju National University College of Medicine, Jeju 690 756, Korea
    Exp Mol Med 38:310-9. 2006
    ..Taken together, our in vivo data suggest that MARCKS is closely linked to microglial activation processes, which are important in pathological conditions, such as neuroinflammation and neurodegeneration...
  81. ncbi request reprint Transmembrane TNF protects mutant mice against intracellular bacterial infections, chronic inflammation and autoimmunity
    Lena Alexopoulou
    Institute of Immunology, Biomedical Sciences Research Center Alexander Fleming, Vari, Greece
    Eur J Immunol 36:2768-80. 2006
    ..These data support the hypothesis that selective targeting of soluble TNF may offer several advantages over complete blockade of TNF in the treatment of chronic inflammation and autoimmunity...
  82. pmc Substrate dependence of conformational changes in the RNA-binding domain of tristetraprolin assessed by fluorescence spectroscopy of tryptophan mutants
    Brandy Y Brewer
    Department of Biochemistry and Molecular Biology and Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Biochemistry 45:13807-17. 2006
    ....
  83. ncbi request reprint RNA sequence elements required for high affinity binding by the zinc finger domain of tristetraprolin: conformational changes coupled to the bipartite nature of Au-rich MRNA-destabilizing motifs
    Brandy Y Brewer
    Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 108 N Greene Street, Baltimore, MD 21201, USA
    J Biol Chem 279:27870-7. 2004
    ..Linkage of conformational changes and binding affinity to the presence and spacing of these adenylate residues provides a thermodynamic basis for the RNA substrate specificity of TTP...
  84. ncbi request reprint Regulation of suppressor of cytokine signaling 3 (SOCS3) mRNA stability by TNF-alpha involves activation of the MKK6/p38MAPK/MK2 cascade
    Christian Ehlting
    Department of Gastroenterology, Hepatology and Infectiology, Heinrich Heine University, Moorenstrasse 5, Dusseldorf, Germany
    J Immunol 178:2813-26. 2007
    ....
  85. ncbi request reprint HuR as a negative posttranscriptional modulator in inflammation
    Vicky Katsanou
    Institute of Immunology, Biomedical Sciences Research Center Alexander Fleming, 16672 Vari, Greece
    Mol Cell 19:777-89. 2005
    ..Our data suggest that HuR acts in a pleiotropic fashion in inflammation through its functional interactions with specific mRNA subsets and negative posttranscriptional modules...
  86. ncbi request reprint Tristetraprolin, a negative regulator of mRNA stability, is increased in old B cells and is involved in the degradation of E47 mRNA
    Daniela Frasca
    Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, FL 33101, USA
    J Immunol 179:918-27. 2007
    ..This is the first report showing that TTP is involved in the degradation of the E47 mRNA and is up-regulated in old B cells...
  87. pmc Myristoylated alanine rich C kinase substrate (MARCKS) heterozygous mutant mice exhibit deficits in hippocampal mossy fiber-CA3 long-term potentiation
    Rifat J Hussain
    Department of Psychiatry, University of Pennsylvania School of Medicine, Clinical Research Building, Philadelphia, Pennsylvania, USA
    Hippocampus 16:495-503. 2006
    ..The implications of these findings for the role of the mossy fiber-CA3 pathway in hippocampus-dependent learning processes are discussed...
  88. pmc Novel mRNA targets for tristetraprolin (TTP) identified by global analysis of stabilized transcripts in TTP-deficient fibroblasts
    Wi S Lai
    Laboratory of Neurobiology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Mol Cell Biol 26:9196-208. 2006
    ..These studies have identified several new, physiologically relevant TTP target transcripts in fibroblasts; these target mRNAs encode proteins from a variety of functional classes...