Affiliation: National Institute of Standards and Technology
- The relative roles of collagen adhesive receptor DDR2 activation and matrix stiffness on the downregulation of focal adhesion kinase in vascular smooth muscle cellsKiran Bhadriraju
SAIC, Mail Stop 8313, 100 Bureau Drive, Gaithersburg, MD 20899, USA
Biomaterials 30:6687-94. 2009..Our results suggest that the collagen receptor DDR2 is involved in the regulation of FAK levels in vSMC adhered to Type I collagen matrices, and that regulation of FAK levels in these cells appears to be independent of matrix stiffness...
- Quantifying myosin light chain phosphorylation in single adherent cells with automated fluorescence microscopyKiran Bhadriraju
SAIC, Arlington, VA, USA
BMC Cell Biol 8:43. 2007..This method allows us to concurrently examine cell morphology, cell-cell contact, and myosin light chain diphosphorylation in vascular smooth muscle cells...
- Using surface plasmon resonance imaging to probe dynamic interactions between cells and extracellular matrixAlexander W Peterson
Cell System Science Group, Biochemical Sciences Division, National Institute of Standards and Technology, Gaithersburg, MD, USA
Cytometry A 77:895-903. 2010..With this novel technique, we demonstrate visualization of cell-substratum interactions, and how cell-substratum interactions change over time as cells spread, migrate, and undergo membrane ruffling...
- Cell response to matrix mechanics: focus on collagenAnne L Plant
National Institute of Standards and Technology, Biochemical Science Division, Gaithersburg, MD 20899, USA
Biochim Biophys Acta 1793:893-902. 2009..The temporal response of cells to differences in ECM may provide insight into mechanisms of signal transduction...
- Surface plasmon resonance imaging of cells and surface-associated fibronectinAlexander W Peterson
Cell and Tissue Measurements Group, Biochemical Sciences Division, National Institute of Standards and Technology, Gaithersburg, MD, USA
BMC Cell Biol 10:16. 2009....
- The treatment of collagen fibrils by tissue transglutaminase to promote vascular smooth muscle cell contractile signalingTighe A Spurlin
Biochemical Science Division, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899, USA
Biomaterials 30:5486-96. 2009..The data suggest that TG2 treatment of collagen increases matrix mechanical stiffness, which apparently alters the contractile and proliferative response of vSMC...
- Nanomechanical properties of thin films of type I collagen fibrilsKoo Hyun Chung
Materials Science and Engineering Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
Langmuir 26:3629-36. 2010....
- The stiffness of collagen fibrils influences vascular smooth muscle cell phenotypeDennis P McDaniel
Biochemical Science Division and Manufacturing Metrology Division National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
Biophys J 92:1759-69. 2007..We suggest that increase in the nanoscale rigidity of collagen fibrils can cause these cells to assume a proliferative phenotype...
- Automated live cell imaging of green fluorescent protein degradation in individual fibroblastsMichael Halter
Cell and Tissue Measurements Group, Biochemical Science Division, Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
Cytometry A 71:827-34. 2007..The approach described in this study will assist the quantification and understanding of gene activity within live cells using fluorescent protein reporters...
- The effect of high vacuum on the mechanical properties and bioactivity of collagen fibril matricesChristopher R Anderton
Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD, 20899, USA
Biointerphases 8:2. 2013..These results may have implication for complex ECM matrices such as decellularized scaffolds. ..
- Activation of ROCK by RhoA is regulated by cell adhesion, shape, and cytoskeletal tensionKiran Bhadriraju
Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA
Exp Cell Res 313:3616-23. 2007..These results suggest the existence of a feedback loop between cytoskeletal tension, adhesion maturation, and ROCK signaling that likely contributes to numerous mechanochemical processes...
- Extracellular matrix-dependent myosin dynamics during G1-S phase cell cycle progression in hepatocytesKiran Bhadriraju
Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA
Exp Cell Res 300:259-71. 2004..Overexpression of the cell cycle protein cyclin D1 overcame both ECM-dependent and actomyosin-dependent inhibition of DNA synthesis, suggesting that cyclin D1 is a key event downstream of myosin-dependent cell cycle regulation...
- Engineering amount of cell-cell contact demonstrates biphasic proliferative regulation through RhoA and the actin cytoskeletonDarren S Gray
Department of Biomedical Engineering, Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205, USA
Exp Cell Res 314:2846-54. 2008..In all, these results demonstrate that cell-cell contact signals through VE-cadherin, RhoA, and intracellular tension in the actin cytoskeleton to regulate proliferation...
- Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitmentRowena McBeath
The Cellular and Molecular Medicine Program, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Dev Cell 6:483-95. 2004..These studies demonstrate that mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate...
- Cells lying on a bed of microneedles: an approach to isolate mechanical forceJohn L Tan
Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 100:1484-9. 2003....
- Extracellular matrix- and cytoskeleton-dependent changes in cell shape and stiffnessKiran Bhadriraju
Biomedical Engineering Institute, MMC 609, 420 Delawere Street S E, University of Minnesota, Minneapolis, Minnesota 55455, USA
Exp Cell Res 278:92-100. 2002..They specifically show that normal actin and myosin function is required for hepatocyte spreading and DNA synthesis and demonstrate opposing effects on cell stiffness upon disruption of actin and myosin...