Kiran Bhadriraju

Summary

Affiliation: National Institute of Standards and Technology
Country: USA

Publications

  1. ncbi request reprint The relative roles of collagen adhesive receptor DDR2 activation and matrix stiffness on the downregulation of focal adhesion kinase in vascular smooth muscle cells
    Kiran Bhadriraju
    SAIC, Mail Stop 8313, 100 Bureau Drive, Gaithersburg, MD 20899, USA
    Biomaterials 30:6687-94. 2009
  2. pmc Quantifying myosin light chain phosphorylation in single adherent cells with automated fluorescence microscopy
    Kiran Bhadriraju
    SAIC, Arlington, VA, USA
    BMC Cell Biol 8:43. 2007
  3. doi request reprint Using surface plasmon resonance imaging to probe dynamic interactions between cells and extracellular matrix
    Alexander W Peterson
    Cell System Science Group, Biochemical Sciences Division, National Institute of Standards and Technology, Gaithersburg, MD, USA
    Cytometry A 77:895-903. 2010
  4. doi request reprint Cell response to matrix mechanics: focus on collagen
    Anne L Plant
    National Institute of Standards and Technology, Biochemical Science Division, Gaithersburg, MD 20899, USA
    Biochim Biophys Acta 1793:893-902. 2009
  5. pmc Surface plasmon resonance imaging of cells and surface-associated fibronectin
    Alexander W Peterson
    Cell and Tissue Measurements Group, Biochemical Sciences Division, National Institute of Standards and Technology, Gaithersburg, MD, USA
    BMC Cell Biol 10:16. 2009
  6. doi request reprint The treatment of collagen fibrils by tissue transglutaminase to promote vascular smooth muscle cell contractile signaling
    Tighe A Spurlin
    Biochemical Science Division, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899, USA
    Biomaterials 30:5486-96. 2009
  7. doi request reprint Nanomechanical properties of thin films of type I collagen fibrils
    Koo Hyun Chung
    Materials Science and Engineering Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    Langmuir 26:3629-36. 2010
  8. pmc The stiffness of collagen fibrils influences vascular smooth muscle cell phenotype
    Dennis P McDaniel
    Biochemical Science Division and Manufacturing Metrology Division National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    Biophys J 92:1759-69. 2007
  9. ncbi request reprint Automated live cell imaging of green fluorescent protein degradation in individual fibroblasts
    Michael Halter
    Cell and Tissue Measurements Group, Biochemical Science Division, Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Cytometry A 71:827-34. 2007
  10. doi request reprint The effect of high vacuum on the mechanical properties and bioactivity of collagen fibril matrices
    Christopher R Anderton
    Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD, 20899, USA
    Biointerphases 8:2. 2013

Collaborators

Detail Information

Publications16

  1. ncbi request reprint The relative roles of collagen adhesive receptor DDR2 activation and matrix stiffness on the downregulation of focal adhesion kinase in vascular smooth muscle cells
    Kiran Bhadriraju
    SAIC, Mail Stop 8313, 100 Bureau Drive, Gaithersburg, MD 20899, USA
    Biomaterials 30:6687-94. 2009
    ..Our results suggest that the collagen receptor DDR2 is involved in the regulation of FAK levels in vSMC adhered to Type I collagen matrices, and that regulation of FAK levels in these cells appears to be independent of matrix stiffness...
  2. pmc Quantifying myosin light chain phosphorylation in single adherent cells with automated fluorescence microscopy
    Kiran Bhadriraju
    SAIC, Arlington, VA, USA
    BMC Cell Biol 8:43. 2007
    ..This method allows us to concurrently examine cell morphology, cell-cell contact, and myosin light chain diphosphorylation in vascular smooth muscle cells...
  3. doi request reprint Using surface plasmon resonance imaging to probe dynamic interactions between cells and extracellular matrix
    Alexander W Peterson
    Cell System Science Group, Biochemical Sciences Division, National Institute of Standards and Technology, Gaithersburg, MD, USA
    Cytometry A 77:895-903. 2010
    ..With this novel technique, we demonstrate visualization of cell-substratum interactions, and how cell-substratum interactions change over time as cells spread, migrate, and undergo membrane ruffling...
  4. doi request reprint Cell response to matrix mechanics: focus on collagen
    Anne L Plant
    National Institute of Standards and Technology, Biochemical Science Division, Gaithersburg, MD 20899, USA
    Biochim Biophys Acta 1793:893-902. 2009
    ..The temporal response of cells to differences in ECM may provide insight into mechanisms of signal transduction...
  5. pmc Surface plasmon resonance imaging of cells and surface-associated fibronectin
    Alexander W Peterson
    Cell and Tissue Measurements Group, Biochemical Sciences Division, National Institute of Standards and Technology, Gaithersburg, MD, USA
    BMC Cell Biol 10:16. 2009
    ....
  6. doi request reprint The treatment of collagen fibrils by tissue transglutaminase to promote vascular smooth muscle cell contractile signaling
    Tighe A Spurlin
    Biochemical Science Division, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899, USA
    Biomaterials 30:5486-96. 2009
    ..The data suggest that TG2 treatment of collagen increases matrix mechanical stiffness, which apparently alters the contractile and proliferative response of vSMC...
  7. doi request reprint Nanomechanical properties of thin films of type I collagen fibrils
    Koo Hyun Chung
    Materials Science and Engineering Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    Langmuir 26:3629-36. 2010
    ....
  8. pmc The stiffness of collagen fibrils influences vascular smooth muscle cell phenotype
    Dennis P McDaniel
    Biochemical Science Division and Manufacturing Metrology Division National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    Biophys J 92:1759-69. 2007
    ..We suggest that increase in the nanoscale rigidity of collagen fibrils can cause these cells to assume a proliferative phenotype...
  9. ncbi request reprint Automated live cell imaging of green fluorescent protein degradation in individual fibroblasts
    Michael Halter
    Cell and Tissue Measurements Group, Biochemical Science Division, Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Cytometry A 71:827-34. 2007
    ..The approach described in this study will assist the quantification and understanding of gene activity within live cells using fluorescent protein reporters...
  10. doi request reprint The effect of high vacuum on the mechanical properties and bioactivity of collagen fibril matrices
    Christopher R Anderton
    Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD, 20899, USA
    Biointerphases 8:2. 2013
    ..These results may have implication for complex ECM matrices such as decellularized scaffolds. ..
  11. pmc Activation of ROCK by RhoA is regulated by cell adhesion, shape, and cytoskeletal tension
    Kiran Bhadriraju
    Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA
    Exp Cell Res 313:3616-23. 2007
    ..These results suggest the existence of a feedback loop between cytoskeletal tension, adhesion maturation, and ROCK signaling that likely contributes to numerous mechanochemical processes...
  12. ncbi request reprint Extracellular matrix-dependent myosin dynamics during G1-S phase cell cycle progression in hepatocytes
    Kiran Bhadriraju
    Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA
    Exp Cell Res 300:259-71. 2004
    ..Overexpression of the cell cycle protein cyclin D1 overcame both ECM-dependent and actomyosin-dependent inhibition of DNA synthesis, suggesting that cyclin D1 is a key event downstream of myosin-dependent cell cycle regulation...
  13. pmc Engineering amount of cell-cell contact demonstrates biphasic proliferative regulation through RhoA and the actin cytoskeleton
    Darren S Gray
    Department of Biomedical Engineering, Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205, USA
    Exp Cell Res 314:2846-54. 2008
    ..In all, these results demonstrate that cell-cell contact signals through VE-cadherin, RhoA, and intracellular tension in the actin cytoskeleton to regulate proliferation...
  14. ncbi request reprint Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment
    Rowena McBeath
    The Cellular and Molecular Medicine Program, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Dev Cell 6:483-95. 2004
    ..These studies demonstrate that mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate...
  15. pmc Cells lying on a bed of microneedles: an approach to isolate mechanical force
    John L Tan
    Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 100:1484-9. 2003
    ....
  16. ncbi request reprint Extracellular matrix- and cytoskeleton-dependent changes in cell shape and stiffness
    Kiran Bhadriraju
    Biomedical Engineering Institute, MMC 609, 420 Delawere Street S E, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Exp Cell Res 278:92-100. 2002
    ..They specifically show that normal actin and myosin function is required for hepatocyte spreading and DNA synthesis and demonstrate opposing effects on cell stiffness upon disruption of actin and myosin...