Michael R Betts

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation
    Michael R Betts
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, USA
    J Immunol Methods 281:65-78. 2003
  2. ncbi request reprint The functional profile of primary human antiviral CD8+ T cell effector activity is dictated by cognate peptide concentration
    Michael R Betts
    Immunology Laboratory, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 172:6407-17. 2004
  3. pmc Acquisition of direct antiviral effector functions by CMV-specific CD4+ T lymphocytes with cellular maturation
    Joseph P Casazza
    Immunology Laboratory, Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases NIAID, National Institutes of Health NIH, Bethesda, MD 20892, USA
    J Exp Med 203:2865-77. 2006
  4. pmc Avidity for antigen shapes clonal dominance in CD8+ T cell populations specific for persistent DNA viruses
    David A Price
    Human Immunology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 202:1349-61. 2005
  5. pmc HIV nonprogressors preferentially maintain highly functional HIV-specific CD8+ T cells
    Michael R Betts
    Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 107:4781-9. 2006
  6. pmc Characterization of functional and phenotypic changes in anti-Gag vaccine-induced T cell responses and their role in protection after HIV-1 infection
    Michael R Betts
    Laboratory of Immunology, Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:4512-7. 2005
  7. pmc The cytolytic enzymes granyzme A, granzyme B, and perforin: expression patterns, cell distribution, and their relationship to cell maturity and bright CD57 expression
    Pratip K Chattopadhyay
    Immunotechnology Section, Laboratory of Immunology, Vaccine Research Center, National Institutes of Health, Bethesda, MD, USA
    J Leukoc Biol 85:88-97. 2009
  8. ncbi request reprint Toll-like receptor ligands modulate dendritic cells to augment cytomegalovirus- and HIV-1-specific T cell responses
    Karin Lore
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 3022, USA
    J Immunol 171:4320-8. 2003
  9. pmc Immunologic pressure within class I-restricted cognate human immunodeficiency virus epitopes during highly active antiretroviral therapy
    Joseph P Casazza
    Immunology Laboratory, National Institutes of Health, Bethesda, MD 20892, USA
    J Virol 79:3653-63. 2005
  10. pmc Virological outcome after structured interruption of antiretroviral therapy for human immunodeficiency virus infection is associated with the functional profile of virus-specific CD8+ T cells
    Marybeth Daucher
    National Institute of Allergy and Infectious Diseases, Bldg 10 Rm 11B13, Bethesda, MD 20892, USA
    J Virol 82:4102-14. 2008

Collaborators

Detail Information

Publications31

  1. ncbi request reprint Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation
    Michael R Betts
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, USA
    J Immunol Methods 281:65-78. 2003
    ....
  2. ncbi request reprint The functional profile of primary human antiviral CD8+ T cell effector activity is dictated by cognate peptide concentration
    Michael R Betts
    Immunology Laboratory, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 172:6407-17. 2004
    ..The inherent ability of viruses to induce high or low Ag states may be the primary determinant of the cytokine vs cytolytic nature of the virus-specific CD8(+) T cell response...
  3. pmc Acquisition of direct antiviral effector functions by CMV-specific CD4+ T lymphocytes with cellular maturation
    Joseph P Casazza
    Immunology Laboratory, Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases NIAID, National Institutes of Health NIH, Bethesda, MD 20892, USA
    J Exp Med 203:2865-77. 2006
    ..Thus, mature CMV-specific CD4+ T cells exhibit distinct functional properties reminiscent of antiviral CD8+ T lymphocytes...
  4. pmc Avidity for antigen shapes clonal dominance in CD8+ T cell populations specific for persistent DNA viruses
    David A Price
    Human Immunology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 202:1349-61. 2005
    ..Vaccine strategies that reconstruct these biological processes could generate T cell populations that mediate optimal delivery of antiviral effector function...
  5. pmc HIV nonprogressors preferentially maintain highly functional HIV-specific CD8+ T cells
    Michael R Betts
    Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 107:4781-9. 2006
    ..Thus, rather than quantity or phenotype, the quality of the CD8(+) T-cell functional response serves as an immune correlate of HIV disease progression and a potential qualifying factor for evaluation of HIV vaccine efficacy...
  6. pmc Characterization of functional and phenotypic changes in anti-Gag vaccine-induced T cell responses and their role in protection after HIV-1 infection
    Michael R Betts
    Laboratory of Immunology, Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:4512-7. 2005
    ..These data suggest that control of HIV by vaccine-elicited HIV-specific T cell responses may be difficult, even when the T cell response has those characteristics predicted to provide optimal protection...
  7. pmc The cytolytic enzymes granyzme A, granzyme B, and perforin: expression patterns, cell distribution, and their relationship to cell maturity and bright CD57 expression
    Pratip K Chattopadhyay
    Immunotechnology Section, Laboratory of Immunology, Vaccine Research Center, National Institutes of Health, Bethesda, MD, USA
    J Leukoc Biol 85:88-97. 2009
    ..Thus, the use of CD57 provides a means to easily isolate viable cells with high cytolytic potential, without the need for lethal fixation/permeabilization techniques...
  8. ncbi request reprint Toll-like receptor ligands modulate dendritic cells to augment cytomegalovirus- and HIV-1-specific T cell responses
    Karin Lore
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 3022, USA
    J Immunol 171:4320-8. 2003
    ....
  9. pmc Immunologic pressure within class I-restricted cognate human immunodeficiency virus epitopes during highly active antiretroviral therapy
    Joseph P Casazza
    Immunology Laboratory, National Institutes of Health, Bethesda, MD 20892, USA
    J Virol 79:3653-63. 2005
    ....
  10. pmc Virological outcome after structured interruption of antiretroviral therapy for human immunodeficiency virus infection is associated with the functional profile of virus-specific CD8+ T cells
    Marybeth Daucher
    National Institute of Allergy and Infectious Diseases, Bldg 10 Rm 11B13, Bethesda, MD 20892, USA
    J Virol 82:4102-14. 2008
    ....
  11. pmc Immunization with vaccinia virus induces polyfunctional and phenotypically distinctive CD8(+) T cell responses
    Melissa L Precopio
    Immunology Laboratory, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 204:1405-16. 2007
    ..This quality of the CD8(+) T cell response may be at least partially responsible for the profound efficacy of these vaccines in protection against smallpox and serves as a benchmark against which other vaccines can be evaluated...
  12. pmc Autocrine production of beta-chemokines protects CMV-Specific CD4 T cells from HIV infection
    Joseph P Casazza
    Immunology Laboratory, Vaccine Research Center, NIAID, NIH, Bethesda, Maryland, USA
    PLoS Pathog 5:e1000646. 2009
    ..These data suggest that CD4+ T cells which produce MIP-1alpha and MIP-1beta bind these chemokines in an autocrine fashion which decreases the risk of in vivo HIV infection...
  13. ncbi request reprint Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry
    Pratip K Chattopadhyay
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
    Nat Med 12:972-7. 2006
    ....
  14. ncbi request reprint Prime-boost vaccination with HIV-1 Gag protein and cytosine phosphate guanosine oligodeoxynucleotide, followed by adenovirus, induces sustained and robust humoral and cellular immune responses
    Marc Tritel
    Cellular Immunology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, USA
    J Immunol 171:2538-47. 2003
    ..Together, these data suggest a new immunization approach for elicitation of long term humoral and cellular immune responses...
  15. ncbi request reprint Expression of CD57 defines replicative senescence and antigen-induced apoptotic death of CD8+ T cells
    Jason M Brenchley
    Vaccine Research Center and the Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 101:2711-20. 2003
    ..Thus, our studies define a phenotype associated with replicative senescence in HIV-specific CD8(+) T cells, which may have broad implications to other conditions associated with chronic antigenic stimulation...
  16. ncbi request reprint T cell receptor recognition motifs govern immune escape patterns in acute SIV infection
    David A Price
    Human Immunology Section, Vaccine Research Center, NIAID NIH, Bethesda, MD 20892, USA
    Immunity 21:793-803. 2004
    ..These findings have profound implications for the development of vaccines that elicit T cell immunity to combat pathogens with unstable genomes...
  17. pmc CTLA-4 blockade decreases TGF-beta, IDO, and viral RNA expression in tissues of SIVmac251-infected macaques
    Anna Hryniewicz
    NCI, Bldg 41 Rm D 804, Bethesda, MD 20892 5065, USA
    Blood 108:3834-42. 2006
    ..Therefore, blunting T(reg) function in macaques infected with SIV did not have detrimental virologic effects and may provide a valuable approach to complement ART and therapeutic vaccination in the treatment of HIV-1 infection...
  18. ncbi request reprint HIV preferentially infects HIV-specific CD4+ T cells
    Daniel C Douek
    Vaccine Research Center, NIAID, NIH, Maryland 20892, USA
    Nature 417:95-8. 2002
    ..Furthermore, the phenomenon of HIV specifically infecting the very cells that respond to it adds a cautionary note to the practice of structured therapy interruption...
  19. ncbi request reprint Detection of T-cell degranulation: CD107a and b
    Michael R Betts
    Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Methods Cell Biol 75:497-512. 2004
  20. ncbi request reprint Flow cytometric analysis of vaccine responses: how many colors are enough?
    Mario Roederer
    Immunotechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA
    Clin Immunol 110:199-205. 2004
    ..In this manuscript, we discuss these technologies, with a focus on assisting in the design and implementation of immunogenicity trials for future vaccine efforts...
  21. ncbi request reprint Optimal antigens for HIV vaccines based on CD8+ T response, protein length, and sequence variability
    Michael R Betts
    Vaccine Research Center, NIAID, NIH, Bethesda, Maryland 20892, USA
    DNA Cell Biol 21:665-70. 2002
    ..The accessory proteins Tat, Rev, Vif, Vpr, and Vpu in general all elicit very low CD8+ T cell responses, and this, in combination with their high variability, makes them less attractive as vaccine antigen...
  22. doi request reprint Brilliant violet fluorophores: a new class of ultrabright fluorescent compounds for immunofluorescence experiments
    Pratip K Chattopadhyay
    Immunotechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, Maryland 20892, USA
    Cytometry A 81:456-66. 2012
    ....
  23. ncbi request reprint A novel approach to the analysis of specificity, clonality, and frequency of HIV-specific T cell responses reveals a potential mechanism for control of viral escape
    Daniel C Douek
    Department of Experimental Transplantation and Immunology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 168:3099-104. 2002
    ..Thus, CD8(+) T cells comprising multiple TCR clonotypes may expand in vivo in response to individual epitopes, and may increase the ability of the response to recognize virus escape mutants...
  24. pmc Immediate cytotoxicity but not degranulation distinguishes effector and memory subsets of CD8+ T cells
    Petra Wolint
    Institute for Microbiology, Eidgenossische Technische Hochschule Zurich, Schmelzbergstrasse 7, 8092, Switzerland
    J Exp Med 199:925-36. 2004
    ..Furthermore, these results provide a potential mechanism by which central memory CD8+ T cell-mediated death of antigen-presenting cells within the lymph node is avoided...
  25. doi request reprint Combined effects of IL-12 and electroporation enhances the potency of DNA vaccination in macaques
    Lauren A Hirao
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 422 Curie Boulevard, 505 SCL, Philadelphia, PA 19104, United States
    Vaccine 26:3112-20. 2008
    ..These data suggest that adjuvant and improved delivery methods may be able to overcome previous immunogenicity limitations in DNA vaccine technology...
  26. pmc Activation drives PD-1 expression during vaccine-specific proliferation and following lentiviral infection in macaques
    David A Hokey
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Eur J Immunol 38:1435-45. 2008
    ..Our data indicate that PD-1 expression is increased as a result of T cell activation during a primary immune response as well as during persistent immune activation in macaques...
  27. ncbi request reprint Absence of immunodominant anti-Gag p17 (SL9) responses among Gag CTL-positive, HIV-uninfected vaccine recipients expressing the HLA-A*0201 allele
    Guido Ferrari
    Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
    J Immunol 173:2126-33. 2004
    ....
  28. ncbi request reprint Selective survival of peripheral blood lymphocytes in children with HIV-1 following delivery of an anti-HIV gene to bone marrow CD34(+) cells
    Greg M Podsakoff
    Childrens Hospital Los Angeles, University of Southern California Keck School of Medicine, Los Angeles, CA 90027, USA
    Mol Ther 12:77-86. 2005
    ..These findings indicate that there was a selective survival advantage for PBMC containing the huM10 gene during the time of increased HIV-1 load...
  29. ncbi request reprint Antigen-specific T-cell-mediated immunity after HIV-1 infection: implications for vaccine control of HIV development
    Michael R Betts
    University of Pennsylvania, Department of Microbiology, 522E Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104, USA
    Expert Rev Vaccines 5:505-16. 2006
    ..Furthermore, potential protective characteristics will be proposed that may ultimately be required for an effective vaccine designed to stimulate cellular immunity against HIV-1...
  30. ncbi request reprint Polyfunctional analysis of human t cell responses: importance in vaccine immunogenicity and natural infection
    George Makedonas
    Department of Microbiology, University of Pennsylvania, Philadelphia, PA, USA
    Springer Semin Immunopathol 28:209-19. 2006
  31. ncbi request reprint Ex vivo identification, isolation and analysis of tumor-cytolytic T cells
    Valerie Rubio
    Department of Medicine, Stanford University, 269 Campus Drive, Stanford, California 94305, USA
    Nat Med 9:1377-82. 2003
    ..The ability to rapidly identify and isolate tumor-cytolytic T cells would be very useful in cancer immunotherapy...