Research Topics
Genomes and Genes
| Michel BernierSummaryAffiliation: National Institutes of Health Country: USA Publications
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Detail Information
Publications
Negative regulation of STAT3 protein-mediated cellular respiration by SIRT1 proteinMichel Bernier
Laboratory of Clinical Investigation, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA
J Biol Chem 286:19270-9. 2011..These results have implications for understanding the interplay between STAT3 and SIRT1 in pro-inflammatory conditions...- Filamin A modulates kinase activation and intracellular trafficking of epidermal growth factor receptors in human melanoma cellsJennifer L Fiori
Laboratories of Clinical Investigation, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224 6825, USA
Endocrinology 150:2551-60. 2009..These results suggest the requirement of FLNa for efficient EGFR kinase activation and the sorting of endocytosed receptors into the degradation pathway... - Role of the pleckstrin homology domain of PLCgamma1 in its interaction with the insulin receptorYong Kook Kwon
Diabetes Section, Laboratory of Clinical Investigation, National Institute on Aging, National Institute of Health, Baltimore, MD 21224 6825, USA
J Cell Biol 163:375-84. 2003..Our results show that PLCgamma1 is a thiol-reactive protein whose association with the IR could contribute to the activation of MAPK signaling by insulin... 
Filamin A-mediated down-regulation of the exchange factor Ras-GRF1 correlates with decreased matrix metalloproteinase-9 expression in human melanoma cellsTie Nian Zhu
Diabetes Section, Laboratory of Clinical Investigation, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
J Biol Chem 282:14816-26. 2007..Our results indicate that expression of FLNa regulates constitutive activation of the Ras/ERK pathway partly through a Ras-GRF1 mechanism to modulate the production of MMP-9...- S-glutathionylation impairs signal transducer and activator of transcription 3 activation and signalingYi Xie
Laboratories of Clinical Investigation, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA
Endocrinology 150:1122-31. 2009..These results are the first to show S-glutathionylation of STAT3, a modification that may exert regulatory function in STAT3 signaling... - In vivo biological activity of exendin (1-30)Maire E Doyle
Diabetes Section National Institute on Aging, National Institutes of Health Diabetes Section, National Institute on Aging, National Institutes of Health, Baltimore MD 21224, USA
Endocrine 27:1-9. 2005..We observed no change in beta-cell area, but did see a change in the number of islets with nuclei positive for BrdU [10.7 +/- 1.8% exendin (1-30) vs 6.5 +/- 0.5% control]... - Wnt5A activates the calpain-mediated cleavage of filamin AMichael P O'Connell
Laboratory of Immunology, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA
J Invest Dermatol 129:1782-9. 2009..Our results indicate that Wnt5A activates calpain-1, leading to the cleavage of filamin A, which results in a remodeling of the cytoskeleton and an increase in melanoma cell motility... - Breast cancer resistance protein (BCRP/ABCG2) localises to the nucleus in glioblastoma multiforme cellsPrateek Bhatia
Laboratory of Clinical Investigation, National Institute on Aging NIA NIH, Baltimore, MD, USA
Xenobiotica 42:748-55. 2012..In conclusion, BCRP is expressed in the nuclear extracts of select GBM and astrocytoma cell lines and in a human GBM tumour biopsy. Its presence in the nucleus of cancer cells suggests new role for BCRP in MDR... - Activation of heat shock factor 1 plays a role in pyrrolidine dithiocarbamate-mediated expression of the co-chaperone BAG3Shaoming Song
Laboratory of Clinical Investigation, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA
Int J Biochem Cell Biol 42:1856-63. 2010..These results unravel a previously unrecognized mechanism by which PDTC and related compounds could confer cellular protection against inflammation through HSF1-induced expression of heat shock response genes... - Exendin-4 improves glycemic control, ameliorates brain and pancreatic pathologies, and extends survival in a mouse model of Huntington's diseaseBronwen Martin
Laboratory of Neurosciences, National Institute on Aging Intramural Research Program, Baltimore, Maryland, USA
Diabetes 58:318-28. 2009..Similarly to Huntington's disease patients, mice expressing the mutated human huntingtin protein also exhibit neurodegenerative changes, motor dysfunction, perturbed energy metabolism, and elevated blood glucose levels... - Adipogenic signaling in rat white adipose tissue: modulation by aging and calorie restrictionMin Zhu
Laboratory of Experimental Gerontology, National Institute on Aging, 6200 Seaforth Street, Baltimore, MD 21224, USA
Exp Gerontol 42:733-44. 2007..Overall these results suggest that CR increased the expression of adipogenic factors, and maintained the differentiated state of adipocytes, which is critically important for adiponectin biosynthesis and insulin sensitivity... - Impact of pyrrolidine dithiocarbamate and interleukin-6 on mammalian target of rapamycin complex 1 regulation and global protein translationShaoming Song
Laboratory of Clinical Investigation, National Institute on Aging, National Institutes of Health, Biomedical Research Center, 251 Bayview Boulevard, Suite 100, Baltimore, MD 21224, USA
J Pharmacol Exp Ther 339:905-13. 2011..These results demonstrate a critical effect of PDTC on mTOR complex 1 function and provide evidence that PDTC can reverse IL-6-related signaling via induction of DDIT4... - Ubiquitination is involved in glucose-mediated downregulation of GIP receptors in isletsJie Zhou
Diabetes Section, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA
Am J Physiol Endocrinol Metab 293:E538-47. 2007..These results suggest that the GIP-R is ubiquitated, resulting in downregulation of the actions of GIP... - Pyrrolidine dithiocarbamate inhibits interleukin-6 signaling through impaired STAT3 activation and association with transcriptional coactivators in hepatocytesHua Jun He
Diabetes Section, Laboratory of Clinical Investigation, National Institutes of Health, Baltimore, Maryland 21224, USA
J Biol Chem 281:31369-79. 2006..These studies are consistent with the ability of PDTC to down-regulate IL-6-induced STAT3 activation by altering the stability of STAT3-Hsp90 complex... - Gut-expressed gustducin and taste receptors regulate secretion of glucagon-like peptide-1Hyeung Jin Jang
National Institute on Aging National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
Proc Natl Acad Sci U S A 104:15069-74. 2007..Modulating GLP-1 secretion in gut "taste cells" may provide an important treatment for obesity, diabetes and abnormal gut motility... - The roles of phospholipase C-gamma 1 and actin-binding protein filamin A in signal transduction of the insulin receptorMichel Bernier
Diabetes Section, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA
Vitam Horm 69:221-47. 2004 - Determination of free and protein-bound glutathione in HepG2 cells using capillary electrophoresis with laser-induced fluorescence detectionYan Wang
Laboratory of Clinical Investigation, Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore, MD, USA
J Chromatogr A 1216:3533-7. 2009.... - Capillary electrophoresis-laser-induced fluorescence (CE-LIF) assay for measurement of intracellular D-serine and serine racemase activityNagendra S Singh
Laboratory of Clinical Investigation, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA
Anal Biochem 421:460-6. 2012..Although the SR dimer has been identified as the active form of the enzyme, all four of the tested cell lines expressed enzymatically active SR... - Protein tyrosine phosphatases: potential role in beta-cell insulin signal transductionMichel Bernier
Diabetes Section, Laboratory of Clinical Investigation, National Institute on Aging, National Institutes of Health, Baltimore, MD, USA
Cell Biochem Biophys 40:209-18. 2004..Hence, the development of tissue-specific gene inactivation strategies should facilitate the study of the potential role of PTPases in beta-cell insulin signaling transduction... - Fat-storing multilocular cells expressing CCR5 increase in the thymus with advancing age: potential role for CCR5 ligands on the differentiation and migration of preadipocytesValeria de Mello Coelho
Laboratories of Immunology, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA
Int J Med Sci 7:1-14. 2009..Moreover, we found that adipocyte differentiation appear to be influenced by the proinflammatory chemokines, CCL3, CCL4 and CCL5... - Circulating adiponectin levels increase in rats on caloric restriction: the potential for insulin sensitizationMin Zhu
Laboratory of Experimental Gerontology, Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
Exp Gerontol 39:1049-59. 2004..Taken together, our findings suggest an important role for adiponectin in the beneficial effects of long-term CR... - Triplex targeted genomic crosslinks enter separable deletion and base substitution pathwaysSally Richards
Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Dr, Baltimore, MD 21224, USA
Nucleic Acids Res 33:5382-93. 2005..We conclude that targeted pso-TFO crosslinks can enter genetically distinct mutational routes that resolve to base substitutions or deletions... - Binding of manumycin A inhibits IkappaB kinase beta activityMichel Bernier
Diabetes Section, Bioanalytical Chemistry and Drug Discovery Section, Laboratory of Clinical Investigation, and Laboratory of Experimental Gerontology, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
J Biol Chem 281:2551-61. 2006..Thus, manumycin A with its epoxyquinoid moieties plays an important regulatory function in IKK signaling through pathways distinct from its role as a protein farnesylation inhibitor... - Cannabinoid receptor activation correlates with the proapoptotic action of the β2-adrenergic agonist (R,R')-4-methoxy-1-naphthylfenoterol in HepG2 hepatocarcinoma cellsRajib K Paul
Laboratory of Clinical Investigation, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224 6825, USA
J Pharmacol Exp Ther 343:157-66. 2012..The data indicate that the presence of the naphthyl moiety in MNF results in functional coupling to the CBR pathway, providing one of the first examples of a dually acting β(2)-AR-CBR ligand... - Identification and characterization of estrogen receptor-related receptor alpha and gamma in human glioma and astrocytoma cellsMukesh K Gandhari
Laboratory of Clinical Investigation, Biomedical Research Center, National Institute on Aging, National Institutes of Health, 251 Bayview Boulevard, Suite 100, Baltimore, MD 21224, USA
Mol Cell Endocrinol 315:314-8. 2010..These results indicate that ERRalpha and ERRgamma are differentially expressed in these tumor cell lines and likely contribute to agonist-dependent ERR transcriptional activity... - S-glutathionylation of cysteine 99 in the APE1 protein impairs abasic endonuclease activityYun Jeong Kim
Laboratory of Molecular Gerontology, Biomedical Research Center, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA
J Mol Biol 414:313-26. 2011..This modification is reversible by reducing agents, which restore APE1 incision function. Our studies describe a novel posttranslational modification of APE1 that regulates the DNA repair function of the protein... - The biarylpyrazole compound AM251 alters mitochondrial physiology via proteolytic degradation of ERRαSusan M Krzysik-Walker
Laboratory of Clinical Investigation, National Institute on Aging, NIH, Biomedical Research Center, 251 Bayview Boulevard, Suite 100, Baltimore, Maryland 21224, USA
Mol Pharmacol 83:157-66. 2013..These results reveal a novel molecular mechanism by which AM251 and related compounds alter mitochondrial physiology through destabilization of ERRα... - Initial synthesis and characterization of an alpha7 nicotinic receptor cellular membrane affinity chromatography column: effect of receptor subtype and cell typeRuin Moaddel
Gerontology Research Center, National Institutes on Aging, National Institutes of Health, Baltimore, Maryland 21224 6825, USA
Anal Chem 80:48-54. 2008..The results are also consistent with the observation that the alpha7 nAChR is localized in lipid rafts in both of these cell lines and that the cholate detergent removed cholesterol from these microdomains... - Wortmannin-sensitive pathway is required for insulin-stimulated phosphorylation of inhibitor kappaBalphaSanjay K Pandey
Diabetes Section, Laboratory of Clinical Investigation, National Institute on Aging, NIH, Baltimore, Maryland 21224-6825, USA
Endocrinology 143:375-85. 2002..These data demonstrate that insulin regulates IkappaBalpha function through a distinct low-affinity wortmannin-sensitive pathway... - Initial synthesis and characterization of an immobilized heat shock protein 90 column for online determination of binding affinitiesMichał P Marszałł
Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA
Anal Biochem 373:313-21. 2008..The IC(50) for GM was 2.80+/-0.18 microM, and the relative IC(50) values were 17-AAG>GM>RAD, in agreement with previously reported values and indicating that immobilization had not affected ATPase activity or sensitivity to inhibition... - The importance of the nine-amino acid C-terminal sequence of exendin-4 for binding to the GLP-1 receptor and for biological activityMaire E Doyle
Diabetes Section, 23, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
Regul Pept 114:153-8. 2003..Observations of the cAMP response in an insulinoma cell line show a similar trend for biological activity... - Interaction of filamin A with the insulin receptor alters insulin-dependent activation of the mitogen-activated protein kinase pathwayHua Jun He
Diabetes Section, Laboratory of Clinical Investigation, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
J Biol Chem 278:27096-104. 2003..Taken together, these results indicate that FLNa interacts constitutively with the IR to exert an inhibitory tone along the MAPK activation pathway...