Gregg T Beckham

Summary

Affiliation: National Renewable Energy Laboratory
Country: USA

Publications

  1. Zheng F, Vermaas J, Zheng J, Wang Y, Tu T, Wang X, et al. Activity and Thermostability of GH5 Endoglucanase Chimeras from Mesophilic and Thermophilic Parents. Appl Environ Microbiol. 2019;85: pubmed publisher
    ..This work illustrates the role that structure-guided recombination can play in helping to identify sequence function relationships within GH5 enzymes by supplementing natural diversity with synthetic diversity. ..
  2. Mayes H, Knott B, Crowley M, Broadbelt L, Ståhlberg J, Beckham G. Who's on base? Revealing the catalytic mechanism of inverting family 6 glycoside hydrolases. Chem Sci. 2016;7:5955-5968 pubmed publisher
    ..This work thus elucidates previously elusive steps in the processive catalytic mechanism of this important class of enzymes. ..
  3. Hobdey S, Knott B, Haddad Momeni M, Taylor L, Borisova A, Podkaminer K, et al. Biochemical and Structural Characterizations of Two Dictyostelium Cellobiohydrolases from the Amoebozoa Kingdom Reveal a High Level of Conservation between Distant Phylogenetic Trees of Life. Appl Environ Microbiol. 2016;82:3395-409 pubmed publisher
    ..reesei when tested under equivalent conditions (with added CBM-linker domains) on an industrially relevant substrate. ..
  4. Tumen Velasquez M, Johnson C, Ahmed A, Dominick G, Fulk E, Khanna P, et al. Accelerating pathway evolution by increasing the gene dosage of chromosomal segments. Proc Natl Acad Sci U S A. 2018;115:7105-7110 pubmed publisher
    ..Different variants emerged, including fusions between GcoA and CatA (catechol 1,2-dioxygenase). This study illustrates the power of harnessing chromosomal gene amplification to accelerate the evolution of desirable traits. ..
  5. Prates E, Guan X, Li Y, Wang X, Chaffey P, Skaf M, et al. The impact of O-glycan chemistry on the stability of intrinsically disordered proteins. Chem Sci. 2018;9:3710-3715 pubmed publisher
  6. Guan X, Chaffey P, Zeng C, Greene E, Chen L, Drake M, et al. Molecular-scale features that govern the effects of O-glycosylation on a carbohydrate-binding module. Chem Sci. 2015;6:7185-7189 pubmed publisher
    ..Going forward, this study suggests the possibility of designing proteins with multiple improved properties by simultaneously varying the structures of O-glycans and amino acids local to the glycosylation site...
  7. Ito T, Saikawa K, Kim S, Fujita K, Ishiwata A, Kaeothip S, et al. Crystal structure of glycoside hydrolase family 127 ?-l-arabinofuranosidase from Bifidobacterium longum. Biochem Biophys Res Commun. 2014;447:32-7 pubmed publisher
    ..The residues around the active site are highly conserved among GH127 members. Based on biochemical experiments and quantum mechanical calculations, a possible reaction mechanism involving cysteine as the nucleophile is proposed. ..
  8. Taylor L, Knott B, Baker J, Alahuhta P, Hobdey S, Linger J, et al. Engineering enhanced cellobiohydrolase activity. Nat Commun. 2018;9:1186 pubmed publisher
    ..Overall, these results reveal CBH regions that enable targeted activity improvements. ..
  9. Franden M, Jayakody L, Li W, Wagner N, Cleveland N, Michener W, et al. Engineering Pseudomonas putida KT2440 for efficient ethylene glycol utilization. Metab Eng. 2018;48:197-207 pubmed publisher
    ..putida KT2440 strain for ethylene glycol consumption, which will serve as a foundational strain for further biocatalyst development for applications in the remediation of waste polyester plastics and biomass-derived wastewater streams. ..

More Information

Publications19

  1. Mallinson S, Machovina M, Silveira R, Garcia Borràs M, Gallup N, Johnson C, et al. A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion. Nat Commun. 2018;9:2487 pubmed publisher
    ..Structural, biochemical, and computational studies of this novel two-component system elucidate the mechanism of its broad substrate specificity, presenting it as a new tool for a critical step in biological lignin conversion. ..
  2. Salvachúa D, Smith H, St John P, Mohagheghi A, Peterson D, Black B, et al. Succinic acid production from lignocellulosic hydrolysate by Basfia succiniciproducens. Bioresour Technol. 2016;214:558-566 pubmed publisher
    ..69g/g, and an overall productivity of 0.43g/L/h. These results demonstrate that B. succiniciproducens may be an attractive platform organism for bio-SA production from biomass hydrolysates. ..
  3. Gudmundsson M, Kim S, Wu M, Ishida T, Momeni M, Vaaje Kolstad G, et al. Structural and electronic snapshots during the transition from a Cu(II) to Cu(I) metal center of a lytic polysaccharide monooxygenase by X-ray photoreduction. J Biol Chem. 2014;289:18782-92 pubmed publisher
    ..Overall, this study provides molecular snapshots along the reduction process to activate the LPMO catalytic machinery and provides a general method for solving LPMO structures in both copper oxidation states. ..
  4. Beckham G, Johnson C, Karp E, Salvachúa D, Vardon D. Opportunities and challenges in biological lignin valorization. Curr Opin Biotechnol. 2016;42:40-53 pubmed publisher
    ..Ultimately, for biological solutions to lignin valorization to be viable, multiple questions in each of these areas will need to be addressed, making biological lignin valorization a multidisciplinary, co-design problem. ..
  5. Guarnieri M, Chou Y, Salvachúa D, Mohagheghi A, St John P, Peterson D, et al. Metabolic Engineering of Actinobacillus succinogenes Provides Insights into Succinic Acid Biosynthesis. Appl Environ Microbiol. 2017;83: pubmed publisher
    ..succinogenes metabolism. ..
  6. Beckham G, Matthews J, Bomble Y, Bu L, Adney W, Himmel M, et al. Identification of amino acids responsible for processivity in a Family 1 carbohydrate-binding module from a fungal cellulase. J Phys Chem B. 2010;114:1447-53 pubmed publisher
    ..Overall, this work provides further insight into the molecular-level mechanisms by which a CBM recognizes and interacts with cellulose. ..
  7. Karp E, Eaton T, Sànchez I Nogué V, Vorotnikov V, Biddy M, Tan E, et al. Renewable acrylonitrile production. Science. 2017;358:1307-1310 pubmed publisher
    ..Avoidance of hydrogen cyanide as a by-product also improves process safety and mitigates product handling requirements. ..
  8. Amore A, Knott B, Supekar N, Shajahan A, Azadi P, Zhao P, et al. Distinct roles of N- and O-glycans in cellulase activity and stability. Proc Natl Acad Sci U S A. 2017;114:13667-13672 pubmed publisher
    ..Overall, this study reveals key roles for N- and O-glycosylation that are likely broadly applicable to other plant cell-wall-degrading enzymes. ..
  9. Linger J, Vardon D, Guarnieri M, Karp E, Hunsinger G, Franden M, et al. Lignin valorization through integrated biological funneling and chemical catalysis. Proc Natl Acad Sci U S A. 2014;111:12013-8 pubmed publisher
    ..Overall, this work demonstrates that the use of aromatic catabolic pathways enables an approach to valorize lignin by overcoming its inherent heterogeneity to produce fuels, chemicals, and materials. ..
  10. Johnson C, Beckham G. Aromatic catabolic pathway selection for optimal production of pyruvate and lactate from lignin. Metab Eng. 2015;28:240-7 pubmed publisher
    ..1 ± 2.6% (wt/wt). Overall, this study illustrates how aromatic degradation pathways can be tuned to optimize the yield of a desired product in biological lignin upgrading. ..