S Patricia Becerra

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Effects of human recombinant PEDF protein and PEDF-derived peptide 34-mer on choroidal neovascularization
    Juan Amaral
    Section of Protein Structure and Function, Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892 0608, USA
    Invest Ophthalmol Vis Sci 51:1318-26. 2010
  2. pmc The effects of PEDF on cancer biology: mechanisms of action and therapeutic potential
    S Patricia Becerra
    National Eye Institute, US National Institutes of Health, Bethesda, Maryland, USA
    Nat Rev Cancer 13:258-71. 2013
  3. ncbi request reprint Focus on Molecules: Pigment epithelium-derived factor (PEDF)
    S Patricia Becerra
    NIH NEI, 7 Memorial Drive, Room 304, Building 7, Bethesda, MD 20892, USA
    Exp Eye Res 82:739-40. 2006
  4. pmc Pigment epithelium-derived factor binds to hyaluronan. Mapping of a hyaluronan binding site
    S Patricia Becerra
    NEI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 283:33310-20. 2008
  5. ncbi request reprint Pigment epithelium-derived factor in the monkey retinal pigment epithelium and interphotoreceptor matrix: apical secretion and distribution
    S Patricia Becerra
    Laboratory of Retinal Cell and Molecular Biology, NEI, NIH, Building 6, Room 308, 6 Center Drive, MSC 2740, Bethesda, MD 20892 0706, USA
    Exp Eye Res 78:223-34. 2004
  6. pmc Pigment epithelium-derived factor (PEDF) prevents retinal cell death via PEDF Receptor (PEDF-R): identification of a functional ligand binding site
    Preeti Subramanian
    Section of Protein Structure and Function, Laboratory of Retinal Cell and Molecular Biology, NEI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 288:23928-42. 2013
  7. pmc Doxycycline-mediated inhibition of choroidal neovascularization
    Sonia Samtani
    National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892 0706, USA
    Invest Ophthalmol Vis Sci 50:5098-106. 2009
  8. pmc Inhibition of tumor cell surface ATP synthesis by pigment epithelium-derived factor: implications for antitumor activity
    Monika Deshpande
    Section of Protein Structure and Function, Laboratory of Retinal Cell and Molecular Biology, NEI NIH, Bethesda, MD, USA
    Int J Oncol 41:219-27. 2012
  9. ncbi request reprint Transscleral-RPE permeability of PEDF and ovalbumin proteins: implications for subconjunctival protein delivery
    Juan Amaral
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892 0706, USA
    Invest Ophthalmol Vis Sci 46:4383-92. 2005
  10. ncbi request reprint Identification of a lipase-linked cell membrane receptor for pigment epithelium-derived factor
    Luigi Notari
    National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 281:38022-37. 2006

Collaborators

Detail Information

Publications27

  1. pmc Effects of human recombinant PEDF protein and PEDF-derived peptide 34-mer on choroidal neovascularization
    Juan Amaral
    Section of Protein Structure and Function, Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892 0608, USA
    Invest Ophthalmol Vis Sci 51:1318-26. 2010
    ..Here, they examined the effects of PEDF polypeptide fragments on vessel sprouting and on choroidal neovascularization (CNV) after subconjunctival administration...
  2. pmc The effects of PEDF on cancer biology: mechanisms of action and therapeutic potential
    S Patricia Becerra
    National Eye Institute, US National Institutes of Health, Bethesda, Maryland, USA
    Nat Rev Cancer 13:258-71. 2013
    ..If PEDF is to be used for cancer management, a deeper appreciation of its many functions and mechanisms of action is needed...
  3. ncbi request reprint Focus on Molecules: Pigment epithelium-derived factor (PEDF)
    S Patricia Becerra
    NIH NEI, 7 Memorial Drive, Room 304, Building 7, Bethesda, MD 20892, USA
    Exp Eye Res 82:739-40. 2006
  4. pmc Pigment epithelium-derived factor binds to hyaluronan. Mapping of a hyaluronan binding site
    S Patricia Becerra
    NEI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 283:33310-20. 2008
    ..The HA-binding activity of PEDF may contribute to deposition in the extracellular matrix and to its reported antitumor/antimetastatic effects...
  5. ncbi request reprint Pigment epithelium-derived factor in the monkey retinal pigment epithelium and interphotoreceptor matrix: apical secretion and distribution
    S Patricia Becerra
    Laboratory of Retinal Cell and Molecular Biology, NEI, NIH, Building 6, Room 308, 6 Center Drive, MSC 2740, Bethesda, MD 20892 0706, USA
    Exp Eye Res 78:223-34. 2004
    ..PEDF can be a useful marker for RPE polarization and differentiation. The polarization of RPE may be an important mechanism to control PEDF secretion and our results offer interesting possibilities on regulation of PEDF...
  6. pmc Pigment epithelium-derived factor (PEDF) prevents retinal cell death via PEDF Receptor (PEDF-R): identification of a functional ligand binding site
    Preeti Subramanian
    Section of Protein Structure and Function, Laboratory of Retinal Cell and Molecular Biology, NEI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 288:23928-42. 2013
    ..Our findings establish that PEDF-R is required for the survival and antiapoptotic effects of PEDF on retina cells and has determinants for PEDF binding within its L4 ectodomain that are critical for enzymatic stimulation. ..
  7. pmc Doxycycline-mediated inhibition of choroidal neovascularization
    Sonia Samtani
    National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892 0706, USA
    Invest Ophthalmol Vis Sci 50:5098-106. 2009
    ..The authors investigated the effects of doxycycline on choroidal neovascularization (CNV) and regulation of MMP-2 and -9 and pigment epithelium-derived factor (PEDF)...
  8. pmc Inhibition of tumor cell surface ATP synthesis by pigment epithelium-derived factor: implications for antitumor activity
    Monika Deshpande
    Section of Protein Structure and Function, Laboratory of Retinal Cell and Molecular Biology, NEI NIH, Bethesda, MD, USA
    Int J Oncol 41:219-27. 2012
    ..The data strongly suggest a role for the interaction between the 34-mer region of PEDF and tumor cell-surface ATP synthase in promoting tumor cell death...
  9. ncbi request reprint Transscleral-RPE permeability of PEDF and ovalbumin proteins: implications for subconjunctival protein delivery
    Juan Amaral
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892 0706, USA
    Invest Ophthalmol Vis Sci 46:4383-92. 2005
    ..To investigate the in vitro, ex vivo, and in vivo transscleral-retinal pigment epithelium (RPE) permeability of PEDF and structurally related proteins for the exploration of novel routes of protein delivery to the retina...
  10. ncbi request reprint Identification of a lipase-linked cell membrane receptor for pigment epithelium-derived factor
    Luigi Notari
    National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 281:38022-37. 2006
    ....
  11. doi request reprint Pigment epithelium-derived factor receptor (PEDF-R): a plasma membrane-linked phospholipase with PEDF binding affinity
    Preeti Subramanian
    NEI NIH, Bethesda, MD 20892 0608, USA
    Adv Exp Med Biol 664:29-37. 2010
    ..The results suggest a molecular pathway by which PEDF ligand/receptor interactions on the cell surface could generate a cellular signal. These conclusions enhance our understanding of the role of PEDF as a neurotrophic survival factor...
  12. pmc Dexamethasone increases pigment epithelium-derived factor in perfused human eyes
    Elizabeth M Perruccio
    Section of Protein Structure and Function, Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, NIH, Bethesda, Maryland, USA
    Curr Eye Res 33:507-15. 2008
    ..To investigate the effects of dexamethasone (DEX) on pigment epithelium-derived factor (PEDF) cDNA and secreted protein in human trabecular meshwork (TM)...
  13. pmc Sustained subconjunctival protein delivery using a thermosetting gel delivery system
    Erin R Rieke
    Laboratory of Bioengineering and Physical Sciences, National Institute of Biomedical Imaging and Bioengineering, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892 5766, USA
    J Ocul Pharmacol Ther 26:55-64. 2010
    ..Additional experiments were performed to compare in vitro to in vivo ovalbumin release rates from the gel...
  14. ncbi request reprint Mapping the type I collagen-binding site on pigment epithelium-derived factor. Implications for its antiangiogenic activity
    Christina Meyer
    Laboratory of Retinal Cell and Molecular Biology, NEI, National Institutes of Health, Bethesda, Maryland 20892 2740, USA
    J Biol Chem 277:45400-7. 2002
    ..The collagen-binding property of PEDF may play a role in surface localization and modulation of its antiangiogenic effects in the eye and bone...
  15. ncbi request reprint A novel imaging technique for experimental choroidal neovascularization
    Mercedes Campos
    Biological Imaging Core, National Eye Institute, NIH, Bethesda, Maryland 20892 0703, USA
    Invest Ophthalmol Vis Sci 47:5163-70. 2006
    ..The authors developed an imaging technique for visualizing and quantifying morphologic changes associated with experimental laser-induced CNV...
  16. pmc Assays for the antiangiogenic and neurotrophic serpin pigment epithelium-derived factor
    Preeti Subramanian
    Section of Protein Structure and Function, National Eye Institute, NIH, Bethesda, Maryland, USA
    Methods Enzymol 499:183-204. 2011
    ..Here we describe purification protocols, methods to quantify PEDF, and determine interactions with specific molecules, as well as neurotrophic and angiogenesis assays for this multifunctional protein...
  17. ncbi request reprint Pigment epithelium-derived factor is a substrate for matrix metalloproteinase type 2 and type 9: implications for downregulation in hypoxia
    Luigi Notari
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 0607, USA
    Invest Ophthalmol Vis Sci 46:2736-47. 2005
    ..Because hypoxia and VEGF regulate matrix metalloproteinases (MMPs), their effects on PEDF proteolysis were explored...
  18. pmc Opposing effects of pigment epithelium-derived factor on breast cancer cell versus neuronal survival: implication for brain metastasis and metastasis-induced brain damage
    Daniel P Fitzgerald
    Women s Cancer s Section, Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, National Institute of Mental Health, Bethesda, Maryland 20892, USA
    Cancer Res 72:144-53. 2012
    ..Our findings establish PEDF as both a metastatic suppressor and a neuroprotectant in the brain, highlighting its role as a double agent in limiting brain metastasis and its local consequences...
  19. pmc Glycosaminoglycans in human retinoblastoma cells: heparan sulfate, a modulator of the pigment epithelium-derived factor-receptor interactions
    Elena M Alberdi
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, Bethesda, MD 20892, USA
    BMC Biochem 4:1. 2003
    ..Pigment epithelium-derived factor (PEDF) has binding affinity for cell-surface receptors in retinoblastoma cells and for glycosaminoglycans. We investigated the effects of glycosaminoglycans on PEDF-receptor interactions...
  20. doi request reprint Pigment epithelium-derived factor reduces apoptosis and pro-inflammatory cytokine gene expression in a murine model of focal retinal degeneration
    Yujuan Wang
    Section of Protein Structure and Function, Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, U S A
    ASN Neuro 5:e00126. 2013
    ..Our results establish that PEDF potently stabilizes photoreceptor degeneration via suppression of both apoptotic and inflammatory pathways. The multiple beneficial effects of PEDF represent a novel approach for potential AMD treatment...
  21. doi request reprint Pigment Epithelium-Derived Factor Protects Cone Photoreceptor-Derived 661W Cells from Light Damage Through Akt Activation
    Matthew Rapp
    National Eye Institute, National Institutes of Health, Bldg 6, Rm 131F, 6 Center Dr, MSC 0608, 20892 0608, Bethesda, MD, USA
    Adv Exp Med Biol 801:813-20. 2014
    ..The results demonstrated that PEDF can protect 661W cells against light-induced cell death and suggest that the binding of PEDF to cell surface PEDF-R triggers a prosurvival signaling pathway. ..
  22. ncbi request reprint Difference in ischemic regulation of vascular endothelial growth factor and pigment epithelium--derived factor in brown norway and sprague dawley rats contributing to different susceptibilities to retinal neovascularization
    Guoquan Gao
    Department of Ophthalmology, Medical University of South Carolina, Charleston, South Carolina 29403, USA
    Diabetes 51:1218-25. 2002
    ..These observations support the idea that different regulation of angiogenic inhibitors and stimulators under ischemia are responsible for the differences in susceptibility to ischemia-induced retinal neovascularization in SD and BN rats...
  23. ncbi request reprint Identification of the neuroprotective molecular region of pigment epithelium-derived factor and its binding sites on motor neurons
    Masako M Bilak
    Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
    J Neurosci 22:9378-86. 2002
    ..The data support the pharmacotherapeutic potential of PEDF as a neuroprotectant in human motor neuron degeneration...
  24. ncbi request reprint Pigment epithelium-derived factor is elevated in CSF of patients with amyotrophic lateral sclerosis
    Ralph W Kuncl
    Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21218, USA
    J Neurochem 81:178-84. 2002
    ..While the mechanism of this presumably reactive increase is not known, the distinctive, surprisingly elevated level of PEDF in the CSF may be an autoprotective reaction in ALS...
  25. ncbi request reprint Micropatterning neuronal cells on polyelectrolyte multilayers
    Darwin R Reyes
    Semiconductor Electronics Division, National Institute of Standards and Technology, 100 Bureau Drive, MS 8120, Gaithersburg, Maryland 20899 8120, USA
    Langmuir 20:8805-11. 2004
    ..This approach provides a simple, fast, and inexpensive method of patterning cells onto micrometer-scale features...
  26. ncbi request reprint Expression of transthyretin and retinol binding protein mRNAs and secretion of transthyretin by cultured monkey retinal pigment epithelium
    Bruce A Pfeffer
    Research, Bausch and Lomb, Inc, Rochester, NY 14609, USA
    Mol Vis 10:23-30. 2004
    ....
  27. pmc Functional genetic analysis of rhesus cytomegalovirus: Rh01 is an epithelial cell tropism factor
    Anders E Lilja
    Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, Princeton, NJ 08544, USA
    J Virol 82:2170-81. 2008
    ..We conclude that Rh01 is a cell tropism determinant that has the potential to dramatically affect virus spread and pathogenesis...