Bruce J Baum

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Inadequate training in the biological sciences and medicine for dental students: an impending crisis for dentistry
    Bruce J Baum
    Gene Transfer Section, Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, U S Department of Health and Human Services, Bethesda, MD 20892 1190, USA
    J Am Dent Assoc 138:16, 18, 20 passim. 2007
  2. pmc Development of a gene transfer-based treatment for radiation-induced salivary hypofunction
    Bruce J Baum
    National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA
    Oral Oncol 46:4-8. 2010
  3. ncbi request reprint The impact of gene therapy on dentistry: a revisiting after six years
    Bruce J Baum
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892 1190, USA
    J Am Dent Assoc 133:35-44. 2002
  4. ncbi request reprint Prospects for re-engineering salivary glands
    B J Baum
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, NIH, Bethesda, MD 20892 1190, USA
    Adv Dent Res 14:84-8. 2000
  5. ncbi request reprint 5.3 Global challenges in research and strategic planning
    Bruce J Baum
    NIDCR, Bethesda, MD, USA
    Eur J Dent Educ 6:179-84. 2002
  6. ncbi request reprint Advances in vector-mediated gene transfer
    Bruce J Baum
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, NIH, DHHS, Building 10, Room 1N113, MSC 1190, Bethesda, MD 20892 1190, USA
    Immunol Lett 90:145-9. 2003
  7. ncbi request reprint Transfer of the AQP1 cDNA for the correction of radiation-induced salivary hypofunction
    Bruce J Baum
    Gene Therapy and Therapeutics Branch, Bethesda, MD 20892 1190, USA
    Biochim Biophys Acta 1758:1071-7. 2006
  8. ncbi request reprint Can biomedical science be made relevant in dental education? A North American perspective
    Bruce J Baum
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, NIH, Bethesda, MD 20892 110, USA
    Eur J Dent Educ 7:49-55; discussion 56-9. 2003
  9. ncbi request reprint Salivary glands as a potential gene transfer target for gene therapeutics of some monogenetic endocrine disorders
    Antonis Voutetakis
    Gene Therapy and Therapeutics Branch, NIDCR, NIH, DHHS, Bethesda, Maryland 20892, USA
    J Endocrinol 185:363-72. 2005
  10. pmc Prevention of radiation-induced oral mucositis after adenoviral vector-mediated transfer of the keratinocyte growth factor cDNA to mouse submandibular glands
    Changyu Zheng
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Cancer Institute, Bethesda, Maryland, USA
    Clin Cancer Res 15:4641-8. 2009

Collaborators

Detail Information

Publications111 found, 100 shown here

  1. ncbi request reprint Inadequate training in the biological sciences and medicine for dental students: an impending crisis for dentistry
    Bruce J Baum
    Gene Transfer Section, Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, U S Department of Health and Human Services, Bethesda, MD 20892 1190, USA
    J Am Dent Assoc 138:16, 18, 20 passim. 2007
  2. pmc Development of a gene transfer-based treatment for radiation-induced salivary hypofunction
    Bruce J Baum
    National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA
    Oral Oncol 46:4-8. 2010
    ..A Phase I/II clinical trial, using a recombinant adenoviral vector to mediate gene transfer, is currently underway...
  3. ncbi request reprint The impact of gene therapy on dentistry: a revisiting after six years
    Bruce J Baum
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892 1190, USA
    J Am Dent Assoc 133:35-44. 2002
    ..The procedure involves the transfer of genes to patients for clinical benefit. Transferred genes can b e used for either reparative or pharmacological purposes...
  4. ncbi request reprint Prospects for re-engineering salivary glands
    B J Baum
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, NIH, Bethesda, MD 20892 1190, USA
    Adv Dent Res 14:84-8. 2000
    ..This report will examine the applications of gene therapy and tissue engineering to salivary glands, with a focus on the repair of irreversible gland damage...
  5. ncbi request reprint 5.3 Global challenges in research and strategic planning
    Bruce J Baum
    NIDCR, Bethesda, MD, USA
    Eur J Dent Educ 6:179-84. 2002
    ..Global networks for mutual information exchange are imperative to overcome resource limitations in individual institutions, as is dedicated funding for research in the dental educational setting...
  6. ncbi request reprint Advances in vector-mediated gene transfer
    Bruce J Baum
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, NIH, DHHS, Building 10, Room 1N113, MSC 1190, Bethesda, MD 20892 1190, USA
    Immunol Lett 90:145-9. 2003
    ..The status of relevant pre-clinical gene transfer studies is reviewed, with an emphasis on use of adenoviral and adeno-associated viral vectors. Current limitations of effective organ-directed gene transfer are also discussed...
  7. ncbi request reprint Transfer of the AQP1 cDNA for the correction of radiation-induced salivary hypofunction
    Bruce J Baum
    Gene Therapy and Therapeutics Branch, Bethesda, MD 20892 1190, USA
    Biochim Biophys Acta 1758:1071-7. 2006
    ..Based on these results, we are developing a clinical trial to test if the hAQP1 cDNA transfer strategy will be clinically effective in restoring salivary flow in patients with IR-induced parotid hypofunction...
  8. ncbi request reprint Can biomedical science be made relevant in dental education? A North American perspective
    Bruce J Baum
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, NIH, Bethesda, MD 20892 110, USA
    Eur J Dent Educ 7:49-55; discussion 56-9. 2003
  9. ncbi request reprint Salivary glands as a potential gene transfer target for gene therapeutics of some monogenetic endocrine disorders
    Antonis Voutetakis
    Gene Therapy and Therapeutics Branch, NIDCR, NIH, DHHS, Bethesda, Maryland 20892, USA
    J Endocrinol 185:363-72. 2005
    ....
  10. pmc Prevention of radiation-induced oral mucositis after adenoviral vector-mediated transfer of the keratinocyte growth factor cDNA to mouse submandibular glands
    Changyu Zheng
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Cancer Institute, Bethesda, Maryland, USA
    Clin Cancer Res 15:4641-8. 2009
    ..The study aims to evaluate if human keratinocyte growth factor (hKGF), secreted after transduction of murine salivary glands with adenoviral vectors, can prevent oral mucositis resulting from radiation...
  11. ncbi request reprint Extended transgene expression from a nonintegrating adenoviral vector containing retroviral elements
    Changyu Zheng
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892 1190, USA
    Mol Ther 16:1089-97. 2008
    ..A vector such as AdLTR(2)EF1alpha-hEPO may prove useful in clinical applications in which extended, but not "permanent," transgene expression is desirable...
  12. doi request reprint Transient detection of E1-containing adenovirus in saliva after the delivery of a first-generation adenoviral vector to human parotid gland
    Changyu Zheng
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892 1190, USA
    J Gene Med 12:3-10. 2010
    ..One week after the administration of AdhAQP1 to an enrolled, generally healthy patient, E1-containing adenovirus was detected in parotid saliva...
  13. pmc Reengineered salivary glands are stable endogenous bioreactors for systemic gene therapeutics
    Antonis Voutetakis
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 101:3053-8. 2004
    ..98). These results show that SGs appear to be promising targets with potential clinical applicability for systemic gene therapeutics...
  14. ncbi request reprint Kinetics of tempol for prevention of xerostomia following head and neck irradiation in a mouse model
    Ana P Cotrim
    Gene Therapy and Therapeutics Branch and Biostatistics Core, Division of Clinical Research and Health Promotion, National Institute of Dental and Craniofacial Research, NIH, Bethesda, Maryland 20892, USA
    Clin Cancer Res 11:7564-8. 2005
    ..The purpose of this study was to examine pharmacokinetic characteristics of the stable nitroxide 4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl (tempol) with respect to radioprotection of the salivary glands...
  15. ncbi request reprint Adeno-associated virus serotype 2-mediated gene transfer to the parotid glands of nonhuman primates
    Antonis Voutetakis
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, Department of Human Health Services, Bethesda, MD 20892, USA
    Hum Gene Ther 18:142-50. 2007
    ..We conclude that administration of modest doses of rAAV2 vectors to SGs for therapeutic purposes can be accomplished without significant or permanent injury to the targeted gland or to distant organs of nonhuman primates...
  16. ncbi request reprint Evaluation of viral and mammalian promoters for use in gene delivery to salivary glands
    Changyu Zheng
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, Department of Health and Human Services, National Institutes of Health, Bethesda, MD 20892 1190, USA
    Mol Ther 12:528-36. 2005
    ..0-kb, rAQP5 0.4-kb, and AMY promoters were not cell-type specific in SMGs; however, the Kall promoter was primarily active in ductal cells. These data will facilitate optimal expression cassette design for salivary gland gene transfer...
  17. doi request reprint Sorting behavior of a transgenic erythropoietin-growth hormone fusion protein in murine salivary glands
    Yuval Samuni
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA
    Hum Gene Ther 19:279-86. 2008
    ....
  18. pmc A novel hybrid adenoretroviral vector with more extensive E3 deletion extends transgene expression in submandibular glands
    Changyu Zheng
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, Bethesda, MD 20892, USA
    Hum Gene Ther Methods 23:169-81. 2012
    ....
  19. ncbi request reprint Effect of serotype 5 adenoviral and serotype 2 adeno- associated viral vector-mediated gene transfer to salivary glands on the composition of saliva
    Jianghua Wang
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, DHHS, Bethesda, MD 20892 1190, USA
    Hum Gene Ther 17:455-63. 2006
    ....
  20. ncbi request reprint Re-engineering primary epithelial cells from rhesus monkey parotid glands for use in developing an artificial salivary gland
    Simon D Tran
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892 1190, USA
    Tissue Eng 12:2939-48. 2006
    ..Together these results demonstrate that it is feasible to individually prepare RPG cells for eventual use in a prototype artificial salivary gland...
  21. pmc Systemic delivery of bioactive glucagon-like peptide 1 after adenoviral-mediated gene transfer in the murine salivary gland
    Antonis Voutetakis
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892, USA
    Endocrinology 151:4566-72. 2010
    ....
  22. pmc Prevention of radiation-induced salivary hypofunction following hKGF gene delivery to murine submandibular glands
    Changyu Zheng
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892 1190, USA
    Clin Cancer Res 17:2842-51. 2011
    ..We evaluated the effect of human keratinocyte growth factor (hKGF) gene transfer to murine salivary glands on the prevention of radiation-induced salivary hypofunction...
  23. ncbi request reprint Immune responses following salivary gland administration of recombinant adeno-associated virus serotype 2 vectors
    Marc R Kok
    Gene Therapy and Therapeutics Branch, NIDCR, NIH, DHHS, Bethesda, MD, USA
    J Gene Med 7:432-41. 2005
    ..There are no reported studies on immune responsiveness of rAAV2 vector administration to SGs...
  24. pmc Salivary epithelial cells: an unassuming target site for gene therapeutics
    Paola Perez
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892 1190, USA
    Int J Biochem Cell Biol 42:773-7. 2010
    ....
  25. pmc Sorting of transgenic secretory proteins in rhesus macaque parotid glands after adenovirus-mediated gene transfer
    Antonis Voutetakis
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, Department of Health and Human Services, Bethesda, MD 20892, USA
    Hum Gene Ther 19:1401-5. 2008
    ..We conclude that RSP proteins are faithfully secreted into saliva in all model species tested, whereas patterns of CSP protein secretion are variable...
  26. ncbi request reprint Toxicity and biodistribution of a first-generation recombinant adenoviral vector, encoding aquaporin-1, after retroductal delivery to a single rat submandibular gland
    Changyu Zheng
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
    Hum Gene Ther 17:1122-33. 2006
    ..In aggregate, these findings show that localized delivery of AdhAQP1 to salivary glands appears to occur without significant toxicity...
  27. ncbi request reprint Modifying the NH2 and COOH termini of aquaporin-5: effects on localization in polarized epithelial cells
    Robert B Wellner
    Gene Transfer Section, Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892 1190, USA
    Tissue Eng 11:1449-58. 2005
    ....
  28. ncbi request reprint Gene therapy of salivary diseases
    Bruce J Baum
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 666:3-20. 2010
    ....
  29. ncbi request reprint Partial redirection of transgenic human growth hormone secretion from rat salivary glands
    Jianghua Wang
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA
    Hum Gene Ther 16:571-83. 2005
    ..03). Although this mutant is unlikely to be therapeutically beneficial, these results suggest that the final destination of a transgenic secretory protein may be controlled by reengineering its sorting determinants...
  30. pmc Early responses to adenoviral-mediated transfer of the aquaporin-1 cDNA for radiation-induced salivary hypofunction
    Bruce J Baum
    Molecular Physiology and Therapeutics Branch, and Neurobiology and Pain Therapeutics Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 109:19403-7. 2012
    ..AdhAQP1 vector delivery to a single parotid gland was safe and transfer of the hAQP1 cDNA increased parotid flow and relieved symptoms in a subset of subjects...
  31. pmc Sorting of growth hormone-erythropoietin fusion proteins in rat salivary glands
    Yuval Samuni
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, USA
    Biochem Biophys Res Commun 373:136-9. 2008
    ..0001 and p=0.0083, respectively). These in vivo studies demonstrate for the first time that hGH, in an N- as well as C-terminal position, influences the secretion of a constitutive pathway protein...
  32. pmc Conditional overexpression of TGF-beta1 disrupts mouse salivary gland development and function
    Bradford E Hall
    Functional Genomics Section, Laboratory of Cell and Developmental Biology, National Institutes of Health, Bethesda, MD 20892, USA
    Lab Invest 90:543-55. 2010
    ....
  33. ncbi request reprint Enhanced transduction of mouse salivary glands with AAV5-based vectors
    H Katano
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD 20892, USA
    Gene Ther 13:594-601. 2006
    ....
  34. pmc Expression and secretion of human proinsulin-B10 from mouse salivary glands: implications for the treatment of type I diabetes mellitus
    Anne M Rowzee
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA
    PLoS ONE 8:e59222. 2013
    ..The ability to remediate a diabetic phenotype in a model of type 1 diabetes mellitus is the first step in an effort that may lead to a possible therapy for diabetes...
  35. ncbi request reprint Inclusion of Moloney murine leukemia virus elements upstream of the transgene cassette in an E1-deleted adenovirus leads to an unusual genomic integration in epithelial cells
    Changyu Zheng
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892 1190, USA
    Virology 313:460-72. 2003
    ..These results suggest that the 2.7 kb of MoMLV sequence included in AdELP-luc have cis-acting functions and mediates an unusual integration event...
  36. pmc Radioprotectors and mitigators of radiation-induced normal tissue injury
    Deborah Citrin
    Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Oncologist 15:360-71. 2010
    ..Few agents are approved for clinical use, but many new compounds show promising results in preclinical testing...
  37. pmc Production and sorting of transgenic, modified human parathyroid hormone in vivo in rat salivary glands
    Janik Adriaansen
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892 1190, USA
    Biochem Biophys Res Commun 391:768-72. 2010
    ....
  38. ncbi request reprint Differential radiation protection of salivary glands versus tumor by Tempol with accompanying tissue assessment of Tempol by magnetic resonance imaging
    Ana P Cotrim
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, NIH, Bethesda, Maryland 20892, USA
    Clin Cancer Res 13:4928-33. 2007
    ..Mechanistic information was explored by monitoring the presence and bioreduction of Tempol in both tissues noninvasively by magnetic resonance imaging (MRI)...
  39. pmc In vivo secretion of the mouse immunoglobulin G Fc fragment from rat submandibular glands
    Gabor Z Racz
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA
    J Gene Med 11:580-7. 2009
    ..In the present study, we studied in vivo the sorting of the mouse immunoglobulin G2b Fc fragment, which is physiologically secreted via the constitutive pathway...
  40. ncbi request reprint Synergy between genetic and tissue engineering: creating an artificial salivary gland
    Bruce J Baum
    Gene Transfer Section, NIDCR, National Institute of Health, Bethesda, Maryland, USA
    Periodontol 2000 41:218-23. 2006
  41. pmc Evaluation of promoters for use in tissue-specific gene delivery
    Changyu Zheng
    Gene Therapy and Therapeutics Branch, NIDCR, NIH, DHHS, Bethesda, MD, USA
    Methods Mol Biol 434:205-19. 2008
    ..Lastly, have a suitable transfection method to test the plasmid constructs in both the in vitro and the in vivo models...
  42. pmc Assessment of the safety and biodistribution of a regulated AAV2 gene transfer vector after delivery to murine submandibular glands
    Changyu Zheng
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland, 20892, USA
    Toxicol Sci 123:247-55. 2011
    ..Overall, our results are consistent with the notion that administration of the AAV2-TF2.3w-hEpo vector to salivary glands posed no significant risk in mice...
  43. ncbi request reprint The use of gene transfer for the protection and repair of salivary glands
    J M Vitolo
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
    Oral Dis 8:183-91. 2002
  44. ncbi request reprint Local adeno-associated virus-mediated interleukin 10 gene transfer has disease-modifying effects in a murine model of Sjögren's syndrome
    Marc R Kok
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
    Hum Gene Ther 14:1605-18. 2003
    ....
  45. pmc Pharmacological protection from radiation ± cisplatin-induced oral mucositis
    Ana P Cotrim
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892 1190, USA
    Int J Radiat Oncol Biol Phys 83:1284-90. 2012
    ..To evaluate if two pharmacological agents, Tempol and D-methionine (D-met), are able to prevent oral mucositis in mice after exposure to ionizing radiation ± cisplatin...
  46. doi request reprint Gene therapy: some history, applications, problems, and prospects
    Ana P Cotrim
    National Institute of Dental and Craniofacial Research, NIH, DHHS, Bethesda, Maryland 20892 1190, USA
    Toxicol Pathol 36:97-103. 2008
    ..There is justifiable optimism that with increased pathobiological understanding and biotechnological improvements, gene therapy will become a standard part of clinical practice within 20 years...
  47. ncbi request reprint Cationic liposome-mediated gene transfer to rat salivary epithelial cells in vitro and in vivo
    L Baccaglini
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892-1190, USA
    J Gene Med 3:82-90. 2001
    ..5%) than those achieved with adenoviral vectors (10(8) PFU). Nonetheless, cationic liposome-mediated gene transfer to salivary glands may be useful for potential therapeutic applications...
  48. ncbi request reprint Salivary glands: novel target sites for gene therapeutics
    Bruce J Baum
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, DHHS, Bethesda, MD 20892 1190, USA
    Trends Mol Med 10:585-90. 2004
    ..Based on pre-clinical studies, we suggest that salivary glands are a valuable, yet under-appreciated, target tissue for both systemic and upper gastrointestinal tract gene therapeutic applications...
  49. ncbi request reprint Integration efficiency of a hybrid adenoretroviral vector
    Changyu Zheng
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bldg 10, Room 1N113, MSC 1190, Bethesda, MD 20892 1190, USA
    Biochem Biophys Res Commun 300:115-20. 2003
    ..These data provide the first quantitative evidence of the frequency for genomic integration with this hybrid vector...
  50. ncbi request reprint Gene transfer to salivary glands
    Bruce J Baum
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892, USA
    Int Rev Cytol 213:93-146. 2002
    ..Several examples are given, using adenoviral-mediated gene transfer, for addressing both biological and clinical questions. Additionally, benefits and shortcomings affecting the utility of this technology are discussed...
  51. ncbi request reprint Salivary gland gene therapy
    Ana P Cotrim
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892 1190, USA
    Dent Clin North Am 50:157-73, vii. 2006
    ....
  52. ncbi request reprint Hydroxychloroquine enhances the endocrine secretion of adenovirus-directed growth hormone from rat submandibular glands in vivo
    A T Hoque
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, MSC-1190, Bethesda, MD 20892, USA
    Hum Gene Ther 12:1333-41. 2001
    ..We conclude that HCQ is able to significantly enhance hGH secretion from salivary glands into the bloodstream and may be useful to facilitate clinical applications of gene therapeutics via salivary glands...
  53. ncbi request reprint Rapamycin control of exocrine protein levels in saliva after adenoviral vector-mediated gene transfer
    J Wang
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, DHHS, Bethesda, MD 20892 1190, USA
    Gene Ther 11:729-33. 2004
    ....
  54. ncbi request reprint Rapamycin control of transgene expression from a single AAV vector in mouse salivary glands
    J Wang
    Gene Therapy and Therapeutics Branch, NIDCR, NIH, DHHS, Bethesda, MD 20892, USA
    Gene Ther 13:187-90. 2006
    ..Our results suggest that the rapamycin transcriptional regulation system delivered in a single AAV2 vector to SGs may be valuable for systemic protein replacement applications...
  55. ncbi request reprint 5.0 Introduction to Theme 5: New challenges and strategic planning in research
    Bruce J Baum
    NIDCR, USA
    Eur J Dent Educ 6:161. 2002
  56. ncbi request reprint Further evidence for AQP8 expression in the myoepithelium of rat submandibular and parotid glands
    Robert B Wellner
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, DHHS, 10 Center Drive, Bldg 10, Rm 1N113, MSC 1190, Bethesda, MD 20892, USA
    Pflugers Arch 451:642-5. 2006
    ..Am J Physiol Renal Physiol 281:F1047-F1057, 2001), we report that AQP8 is expressed in the myoepithelial cells, but not in the acinar cells, of both the rat SMG and PG...
  57. ncbi request reprint Etanercept in Sjögren's syndrome: a twelve-week randomized, double-blind, placebo-controlled pilot clinical trial
    Vidya Sankar
    National Institutes of Health, Bethesda, Maryland, USA
    Arthritis Rheum 50:2240-5. 2004
    ..To assess the safety and potential efficacy of etanercept in the treatment of Sjögren's syndrome (SS)...
  58. ncbi request reprint Localization of AQP5/AQP8 chimeras in MDCK-II cells: exchange of the N- and C-termini
    Robert B Wellner
    Gene Transfer Section, Gene Therapy and Therapeutics Branch, National Institute of Dental and Cranciofacial Research, National Institutes of Health, DHHS, Bethesda, MD 20892, USA
    Biochem Biophys Res Commun 330:172-7. 2005
    ..Our results suggest that, unlike the other AQP targeting/retention signals reported thus far, an AQP8 basolateral targeting/retention motif might reside between the two cytosolic termini...
  59. ncbi request reprint Distribution and toxicity resulting from adenoviral vector administration to a single salivary gland in adult rats
    Brian C O'Connell
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, Bethesda, MD 20892, USA
    J Oral Pathol Med 32:414-21. 2003
    ..We examined the distribution and toxicity associated with a single salivary gland administration of a recombinant adenoviral vector, AdCMVH3, encoding human histatin 3...
  60. ncbi request reprint The stable nitroxide tempol facilitates salivary gland protection during head and neck irradiation in a mouse model
    Joseph M Vitolo
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, NIH, Bethesda, Maryland 20892, USA
    Clin Cancer Res 10:1807-12. 2004
    ..The purpose of this study was to test the ability of the stable-free radical Tempol (4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl), an established radioprotector, to prevent radiation-induced salivary hypofunction in mice...
  61. ncbi request reprint Gender differences in serotype 2 adeno-associated virus biodistribution after administration to rodent salivary glands
    A Voutetakis
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
    Hum Gene Ther 18:1109-18. 2007
    ..Sexual dimorphism is a factor of major significance that could potentially affect gene therapy clinical applications in SGs...
  62. ncbi request reprint Toxicity and biodistribution of a first-generation recombinant adenoviral vector, in the presence of hydroxychloroquine, following retroductal delivery to a single rat submandibular gland
    C Zheng
    Gene Therapy and Therapeutics Branch, NIDCR, NIH, DHHS, Bethesda, MD 20892 1190, USA
    Oral Dis 12:137-44. 2006
    ..g. Baum et al, 1999), but administration of HCQ substantially increases the hGH levels secreted into the bloodstream (Hoque et al, 2001). A potential application of this observation is for patients with adult hGH deficiency...
  63. ncbi request reprint Utilizing endocrine secretory pathways in salivary glands for systemic gene therapeutics
    Antonis Voutetakis
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, DHHS, Bethesda, Maryland 20892 1190, USA
    J Cell Physiol 199:1-7. 2004
    ..Existing studies suggest that clinical benefits will result from salivary gland targeted systemic gene therapeutics...
  64. ncbi request reprint Expression of the aquaporin 8 water channel in a rat salivary epithelial cell
    A T M Shamsul Hoque
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Cell Physiol 191:336-41. 2002
    ..Similarly, when grown on coverslips and then exposed to a hypertonic solution, SMIE cells shrunk as a function of time. Together, these results suggest that SMIE cells endogenously express functional AQP8 water channels...
  65. ncbi request reprint Female mice are more susceptible to developing inflammatory disorders due to impaired transforming growth factor beta signaling in salivary glands
    Seshagiri R Nandula
    National Institute of Dental and Craniofacial Research, NIH, Bethesda, Maryland 20892, USA
    Arthritis Rheum 56:1798-805. 2007
    ..The aim of this study was to delineate the precise function of TGFbeta signaling in salivary gland inflammation...
  66. ncbi request reprint Prevention of irradiation-induced salivary hypofunction by microvessel protection in mouse salivary glands
    Ana P Cotrim
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, Bethesda, Maryland 20892 1190, USA
    Mol Ther 15:2101-6. 2007
    ..Furthermore, our results suggest that a local transient treatment directed at protecting SG endothelial cells may be beneficial for patients undergoing IR for head and neck cancer...
  67. ncbi request reprint Evaluation of radiation-induced oral mucositis by optical coherence tomography
    Thierry M Muanza
    Radiation Oncology Branch, National Cancer Institute, Bethesda, Maryland 20892, USA
    Clin Cancer Res 11:5121-7. 2005
    ..Optical coherence tomography (OCT) imaging was evaluated to determine if radiation-induced mucosal damage could be noninvasively monitored in real time and correlated with histopathologic findings...
  68. ncbi request reprint Long-term expression after infection by the hybrid vector AdLTR-luc is from integrated transgene
    Changyu Zheng
    Gene Therapy and Therapeutics Branch, National Institutes of Health, Bethesda, Maryland 20892 1190, USA
    Biochem Biophys Res Commun 291:34-40. 2002
    ..Similar analyses of low molecular weight DNA extracts indicated the absence of intact extrachromosomal vector. These data demonstrate that long-term luciferase expression after infection by AdLTR-luc is derived from the integrated cDNA...
  69. pmc AAV5-mediated gene transfer to the parotid glands of non-human primates
    A Voutetakis
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, Bethesda, MD 20892 1190, USA
    Gene Ther 17:50-60. 2010
    ..The aggregate data indicate that results with AAV5 vectors in murine salivary glands apparently do not extend to macaque glands...
  70. ncbi request reprint Using salivary glands as a tissue target for gene therapeutics
    A T Hoque
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, NIH, Bethesda, MD 20892-1190, USA
    J Drug Target 9:485-94. 2001
    ..Ongoing efforts, however, suggest that salivary glands may be suitable as gene therapeutic target sites for drug delivery in the near future...
  71. pmc Transient TWEAK overexpression leads to a general salivary epithelial cell proliferation
    T Sugito
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892 1190, USA
    Oral Dis 15:76-81. 2009
    ..This study evaluated the hypothesis that TWEAK overexpression in salivary glands would lead to the proliferation of a salivary progenitor cell...
  72. ncbi request reprint Recombinant adeno-associated virus serotype 2 vectors mediate stable interleukin 10 secretion from salivary glands into the bloodstream
    Seiichi Yamano
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
    Hum Gene Ther 13:287-98. 2002
    ....
  73. ncbi request reprint Sex steroid hormones in primary Sjögren's syndrome
    Michael T Brennan
    Gene Therapy and Therapeutics Branch GTTB, National Institute of Dental and Craniofacial Research NIDCR, National Institutes of Health, Bethesda, Maryland, USA
    J Rheumatol 30:1267-71. 2003
    ..To investigate the relationships between concentrations of sex hormones and measures of disease activity in patients with primary Sjögren's Syndrome (pSS)...
  74. ncbi request reprint Polarized secretion of transgene products from salivary glands in vivo
    B J Baum
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, NIH, Bethesda, MD 20892, USA
    Hum Gene Ther 10:2789-97. 1999
    ..e., in an endocrine manner. The aggregate results are consistent with the recognition of signals encoded within the transgenes that result in specific patterns of polarized protein secretion from rat submandibular gland cells in vivo...
  75. ncbi request reprint Re-engineering the functions of a terminally differentiated epithelial cell in vivo
    B J Baum
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892, USA
    Ann N Y Acad Sci 875:294-300. 1999
    ..The third series of studies show our general approach to develop an artificial salivary gland for clinical situations in which all glandular tissue has been lost...
  76. ncbi request reprint The dental curriculum: what should be new in the 21st century?
    B J Baum
    Clinical Investigations and Patient Care Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892 1190, USA
    J Public Health Dent 56:286-90. 1996
    ....
  77. ncbi request reprint Polarized sorting of aquaporins 5 and 8 in stable MDCK-II transfectants
    R B Wellner
    Gene Therapy and Therapeutics Branch, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochem Biophys Res Commun 285:1253-8. 2001
    ..This is the first report of (i) stable epithelial cell cultures exhibiting a functional, polarized distribution of AQPs 5 and 8, and (ii) a basolateral localization of AQP8 in a polarized epithelial cell...
  78. ncbi request reprint Evidence that aquaporin-8 is located in the basolateral membrane of rat submandibular gland acinar cells
    R B Wellner
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, Bethesda, MD 20892 1190, USA
    Pflugers Arch 441:49-56. 2000
    ....
  79. ncbi request reprint Gamma-irradiation-induced cell cycle arrest and cell death in a human submandibular gland cell line: effect of E2F1 expression
    A C O'Connell
    Gene Therapy and Therapeutics Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Cell Physiol 177:264-73. 1998
    ....
  80. ncbi request reprint Salivary enhancement: current status and future therapies
    J C Atkinson
    Department of Oral Medicine, University of Maryland Dental School, Baltimore, MD, USA
    J Dent Educ 65:1096-101. 2001
    ..However, these medications do not benefit all patients. The possibilities of using gene therapy and tissue engineering to develop treatments for those with severe salivary dysfunction are discussed...
  81. ncbi request reprint Protection from experimental endotoxemia by a recombinant adeno-associated virus encoding interleukin 10
    S Yamano
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
    J Gene Med 3:450-7. 2001
    ..Interleukin 10 (IL-10) is a homodimeric cytokine that shows considerable clinical promise. Adeno-associated virus (AAV) vectors appear increasingly useful for in vivo gene-transfer applications...
  82. ncbi request reprint Transferring genes to salivary glands
    R K Simmons
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
    J Dent Educ 65:907-10. 2001
    ..Overall, this body of work provides the dental educator with a substantive example of how biotechnological progress will significantly affect the treatment of oral problems in the near future...
  83. ncbi request reprint The need to introduce gene therapy to the dental curriculum
    B J Baum
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, NIH, Bethesda, MD 20892, USA
    Eur J Dent Educ 3:49-51. 1999
    ..The dental curriculum must begin to include the biological basis of DNA-based therapies and other related biomedical science progress...
  84. ncbi request reprint Salivary glands as a model for craniofacial applications of gene transfer
    B J Baum
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892, USA
    Int J Oral Maxillofac Surg 29:163-6. 2000
    ..The purpose of this review is to familiarize the oral and maxillofacial surgeon with this technology. Studies performed with salivary glands in animal models are presented as examples of proof of concept...
  85. pmc Differential sorting of human parathyroid hormone after transduction of mouse and rat salivary glands
    J Adriaansen
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892 1190, USA
    Hum Gene Ther 19:1021-8. 2008
    ..Detailed studies in these two species should result in a better understanding of cellular control of transgenic secretory protein sorting in this tissue...
  86. pmc Use of localised gene transfer to develop new treatment strategies for the salivary component of Sjögren's syndrome
    M R Kok
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, USA
    Ann Rheum Dis 62:1038-46. 2003
    ....
  87. pmc Effect of human vasoactive intestinal peptide gene transfer in a murine model of Sjogren's syndrome
    B M Lodde
    GTTB NIDCR, National Institutes of Health, 10 Center Drive, Room 1N114, MSC 1190, Bethesda, MD 20892 1190, USA
    Ann Rheum Dis 65:195-200. 2006
    ..The aetiology and pathogenesis are largely unknown; currently, only palliative treatment is available...
  88. ncbi request reprint A recombinant adenoviral vector encoding functional vasoactive intestinal peptide
    B M Lodde
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, DHHS, Bethesda, MD 20892 1190, USA
    Biochem Biophys Res Commun 319:189-92. 2004
    ..This is the first report showing the construction of a recombinant viral vector encoding biologically active VIP...
  89. ncbi request reprint Salivary gland delivery of pDNA-cationic lipoplexes elicits systemic immune responses
    V Sankar
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research NIDCR, NIH, Bethesda, MD 20892, USA
    Oral Dis 8:275-81. 2002
    ..To test the ability of two cationic lipoplexes, Vaxfectin and GAP-DLRIE/DOPE, to facilitate transfection and elicit immune responses from plasmid DNAs (pDNAs) after retrograde instillation into salivary glands...
  90. ncbi request reprint Absence of tight junction formation in an allogeneic graft cell line used for developing an engineered artificial salivary gland
    D J Aframian
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Building 10 Rm 1N113, Bethesda, MD 20892, USA
    Tissue Eng 8:871-8. 2002
    ..These studies also emphasize the importance of graft cell selection in artificial organ development, as certain required characteristics may be difficult to reengineer...
  91. ncbi request reprint Primary culture of polarized human salivary epithelial cells for use in developing an artificial salivary gland
    S D Tran
    National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892, USA
    Tissue Eng 11:172-81. 2005
    ..We conclude that this culture method for obtaining autologous human salivary cells should be useful in developing an artificial salivary gland...
  92. ncbi request reprint Evaluation of salivary gland acinar and ductal cell-specific promoters in vivo with recombinant adenoviral vectors
    C Zheng
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, 9000 Rockville Pike, Bldg 10, Room 1N113, Bethesda, MD 20892 1190, USA
    Hum Gene Ther 12:2215-23. 2001
    ..This study demonstrates that adenoviral vectors containing the relatively cell/tissue-specific AMY1C or KLK1 promoters may be useful for targeting therapeutic gene expression in salivary glands...
  93. ncbi request reprint NOD mouse model for Sjögren's syndrome: lack of longitudinal stability
    B M Lodde
    Gene Therapy and Therapeutics Branch NIDCR, NIH, DHHS, Bethesda, MD 20892 1190, USA
    Oral Dis 12:566-72. 2006
    ..Data collected over a 2-year period indicated a changing SS phenotype in these mice and this phenomenon was investigated...
  94. ncbi request reprint Differentiation of human bone marrow-derived cells into buccal epithelial cells in vivo: a molecular analytical study
    Simon D Tran
    National Institute of Dental and Craniofacial Research, Gene Therapy and Therapeutics Branch National Institutes of Health, Bethesda, MD 20892 1190, USA
    Lancet 361:1084-8. 2003
    ..We aimed to determine whether fusion events are important in vivo...
  95. ncbi request reprint Risk factors for positive minor salivary gland biopsy findings in Sjögren's syndrome and dry mouth patients
    Michael T Brennan
    National Institute of Dental and Craniofacial Research NIDCR, NIH, Bethesda, Maryland, USA
    Arthritis Rheum 47:189-95. 2002
    ..To investigate risk factors for positive minor salivary gland biopsy results in Sjögren's syndrome (SS) and dry mouth patients...
  96. pmc Evaluation of a rapamycin-regulated serotype 2 adeno-associated viral vector in macaque parotid glands
    C Zheng
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, Bethesda, MD 20892 1190, USA
    Oral Dis 16:269-77. 2010
    ..This study evaluated if such a vector was similarly useful in rhesus macaque parotid glands...
  97. ncbi request reprint Using HSV-thymidine kinase for safety in an allogeneic salivary graft cell line
    D J Aframian
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892, USA
    Tissue Eng 7:405-13. 2001
    ..These results suggest that engineering the expression of a suicide gene in an allogeneic graft cell may provide additional safety for use in an artificial salivary gland device...
  98. ncbi request reprint Examination of rat salivary glands for the presence of the aquaporin CHIP
    J Li
    Clinical Investigations and Patient Care Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892
    Pflugers Arch 428:455-60. 1994
    ..Further, this study provides strong evidence that AQP-CHIP is an endothelial cell water channel found within salivary gland tissue which may contribute to water permeability in the capillary beds...
  99. ncbi request reprint Interferon-gamma-induced JAK2 and STAT1 signalling in a human salivary gland cell line
    A J Wu
    Clinical Investigations and Patient Care Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland, USA
    J Cell Physiol 173:110-4. 1997
    ..These results demonstrate that the JAK2 and Stat1 signalling pathway is active in salivary-derived epithelial cells and may contribute to their immunopathologic destruction...
  100. ncbi request reprint Centromere protein C is a target of autoantibodies in Sjögren's syndrome and is uniformly associated with antibodies to Ro and La
    Stanley R Pillemer
    National Institute of Health, National Institute of Dental and Craniofacial Research, Bethesda, Maryland 20892, USA
    J Rheumatol 31:1121-5. 2004
    ..To determine which centromere proteins are recognized in Sjögren's syndrome (SS) and whether antibodies recognizing centromere proteins (CENP) B and CENP C identify a specific serologic subset...
  101. ncbi request reprint Cellular characteristics of long-term cultured rat parotid acinar cells
    C Yeh
    Clinical Investigations and Patient Care Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892
    In Vitro Cell Dev Biol 27:707-12. 1991
    ..These findings suggest that rat parotid acinar cells can be maintained in a differentiated state in vitro for long periods, and can serve as a useful model system for studying the regulation of exocrine secretory processes...