Research Topics
| T BallaSummaryAffiliation: National Institutes of Health Country: USA Publications
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Publications
Crucial role of phosphatidylinositol 4-kinase IIIalpha in development of zebrafish pectoral fin is linked to phosphoinositide 3-kinase and FGF signalingHui Ma
Section on Molecular Signal Transduction, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
J Cell Sci 122:4303-10. 2009..Our results identify Pik4a as an upstream partner of PI3Ks in the signaling cascade orchestrated by FGF receptors with a prominent role in forelimb development...- Live cell imaging with protein domains capable of recognizing phosphatidylinositol 4,5-bisphosphate; a comparative studyZsofia Szentpetery
Sections on molecular signal transduction, Program for Developmental Neuroscience, NICHD, National Institutes of Health, Bethesda, MD 20892, USA
BMC Cell Biol 10:67. 2009..Here we report on a comparative analysis of several recently-described yeast PH domains as well as the mammalian Tubby domain to evaluate their usefulness as PtdIns(4,5)P2 imaging tools... 
Phosphoinositide-derived messengers in endocrine signalingT Balla
Endocrinology and Reproduction Research Branch, NICHD, National Institutes of Health, Building 49, Room 6A35, 49 Convent Drive, Bethesda, Maryland 20892, USA
J Endocrinol 188:135-53. 2006....- Inositol-lipid binding motifs: signal integrators through protein-lipid and protein-protein interactionsTamas Balla
Endocrinology and Reproduction Research Branch, NICHD, National Institutes of Health, Bethesda, MD 20892, USA
J Cell Sci 118:2093-104. 2005..Therefore, these modules function as crucially important signal integrators, which explains their involvement in a broad range of regulatory functions in eukaryotic cells... 
Design of drug-resistant alleles of type-III phosphatidylinositol 4-kinases using mutagenesis and molecular modelingAndras Balla
Section on Molecular Signal Transduction, Center for Developmental Neuroscience, NICHD, National Institutes of Health, Bethesda, Maryland 20892 4510, USA
Biochemistry 47:1599-607. 2008..These studies should aid development of subtype-specific inhibitors of type-III PI4Ks and help to better understand the significance of localized PtdIns4P production by the various PI4Ks isoforms in specific cellular compartments...- Regulation of Ca2+ entry by inositol lipids in mammalian cells by multiple mechanismsTamas Balla
Section on Molecular Signal Transduction, Program for Developmental Neuroscience, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, United States
Cell Calcium 45:527-34. 2009..Here we summarize the various means by which phosphoinositides affect ion channel functions with special emphasis on Ca(2+) signaling and outline the principles that govern the highly compartmentalized roles of these regulatory lipids... - Finding partners for PI3Kgamma: when 84 is better than 101Tamas Balla
Eunice Kennedy Shriver National Institute of Child Health and Development, National Institutes of Health, 49 Convent Drive, Bethesda, MD 20892, USA
Sci Signal 2:pe35. 2009..This remarkable specificity challenges our views of how phosphoinositides regulate their downstream effectors... - Phosphoinositide signaling: new tools and insightsTamas Balla
Section on Molecular Signal Transduction, Program for Developmental Neuroscience, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
Physiology (Bethesda) 24:231-44. 2009..The recent progress allows us to understand the underlying causes of certain human diseases and design new strategies for therapeutic interventions... - Imaging and manipulating phosphoinositides in living cellsTamas Balla
National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
J Physiol 582:927-37. 2007..This article recalls some historical aspects of inositide research and describes the new methodological advances highlighting their great potential as well as the problems one can encounter with their use... - Green light to illuminate signal transduction eventsTamas Balla
Section on Molecular Signal Transduction, Program on Developmental Neuroscience, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Trends Cell Biol 19:575-86. 2009..Spatial definition of biochemical events followed with real-time temporal resolution has become a standard goal, and several new techniques are now breaking the resolution barrier of light microscopy... - Inositol lipid binding and membrane localization of isolated pleckstrin homology (PH) domains. Studies on the PH domains of phospholipase C delta 1 and p130Peter Varnai
Endocrinology and Reproduction Research Branch, NICHD National Institutes of Health, 49 Convent Drive, Bldg 49, Bethesda, MD 20892, USA
J Biol Chem 277:27412-22. 2002.... - A plasma membrane pool of phosphatidylinositol 4-phosphate is generated by phosphatidylinositol 4-kinase type-III alpha: studies with the PH domains of the oxysterol binding protein and FAPP1Andras Balla
Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Mol Biol Cell 16:1282-95. 2005..Our data suggest that these PH domains detect PI(4)P formation in extra-Golgi compartments under dynamic conditions and that various PI4Ks regulate PI(4)P synthesis in distinct cellular compartments... - Signaling events activated by angiotensin II receptors: what goes before and after the calcium signalsT Balla
Endocrinology and Reproduction Research Branch, National Institutes of Health, Bethesda, MD 20892, USA
Endocr Res 24:335-44. 1998..The use of these tools will help to further clarify the complex role of these lipids in initiating Ca2+-dependent and -independent signaling responses... - The pleckstrin homology domain of phosphoinositide-specific phospholipase Cdelta4 is not a critical determinant of the membrane localization of the enzymeSang Bong Lee
Laboratory of Cell Signaling, NHLI, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 279:24362-71. 2004.... - Interaction of neuronal calcium sensor-1 (NCS-1) with phosphatidylinositol 4-kinase beta stimulates lipid kinase activity and affects membrane trafficking in COS-7 cellsX Zhao
Endocrinology and Reproduction Research Branch, NICHD, National Institutes of Health, 49 Convent Drive, Bethesda, MD 20892, USA
J Biol Chem 276:40183-9. 2001..These results together indicate that NCS-1 is able to interact with PI4Kbeta also in mammalian cells and may play a role in the regulation of this enzyme in specific cellular compartments affecting vesicular trafficking... - Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate levels influence multiple regulatory functions of the lipid in intact living cellsPeter Varnai
Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
J Cell Biol 175:377-82. 2006.... - Structural determinants of Ras-Raf interaction analyzed in live cellsTzvetanka Bondeva
Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-4510, USA
Mol Biol Cell 13:2323-33. 2002..Visualization of activated Ras in live cells will help to better understand the dynamics of Ras activation under various physiological and pathological conditions... - Dependence of STIM1/Orai1-mediated calcium entry on plasma membrane phosphoinositidesMarek K Korzeniowski
Sections on molecular signal transduction, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 284:21027-35. 2009..These results suggest an inositide requirement of Orai1 activation but not STIM1 movements and indicate that PtdIns4P rather than PtdIns(4,5)P2 is a likely determinant of Orai1 channel activity... - Live cell imaging of phosphoinositides with expressed inositide binding protein domainsPeter Varnai
Department of Physiology, Semmelweis University Faculty of Medicine, Budapest, H 1088 Budapest, Puskin utca 9, Hungary, Bethesda, MD 20892, USA
Methods 46:167-76. 2008.... - Targeted expression of the inositol 1,4,5-triphosphate receptor (IP3R) ligand-binding domain releases Ca2+ via endogenous IP3R channelsPeter Varnai
Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 102:7859-64. 2005.... - Stimulation of early gene expression by angiotensin II in bovine adrenal glomerulosa cells: roles of calcium and protein kinase CA J Clark
Endocrinology and Reproduction Research Branch, National Institutes of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892
Mol Endocrinol 6:1889-98. 1992.... - Metabolism of inositol 1,4,5-trisphosphate to higher inositol phosphates in bovine adrenal cytosolG Guillemette
Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892
Am J Hypertens 2:387-94. 1989..The metabolic conversion of inositol 1,4,5-trisphosphate to several higher inositol polyphosphates provides potential new messengers for intracellular regulation in agonist-stimulated target cells... - Visualization and manipulation of plasma membrane-endoplasmic reticulum contact sites indicates the presence of additional molecular components within the STIM1-Orai1 ComplexPeter Varnai
Section on Molecular Signal Transduction, NICHD, National Institutes of Health, Bethesda, Maryland 20892 4510, USA
J Biol Chem 282:29678-90. 2007..These studies are the first to detect juxtaposed areas between the ER and the plasma membrane in live cells, revealing novel details of STIM1-Orai1 interactions... - Active Arf6 recruits ARNO/cytohesin GEFs to the PM by binding their PH domainsLee Ann Cohen
Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, and Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Mol Biol Cell 18:2244-53. 2007..This interaction was direct and required both inositol phospholipids and GTP. We propose a model of sequential Arf activation at the PM whereby Arf6-GTP recruits ARNO family GEFs for further activation of other Arf isoforms... - Maintenance of hormone-sensitive phosphoinositide pools in the plasma membrane requires phosphatidylinositol 4-kinase IIIalphaAndras Balla
Section on Molecular Signal Transduction, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Mol Biol Cell 19:711-21. 2008.... - Selective cellular effects of overexpressed pleckstrin-homology domains that recognize PtdIns(3,4,5)P3 suggest their interaction with protein binding partnersPeter Varnai
Endocrinology and Reproduction Research Branch, National Institutes of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
J Cell Sci 118:4879-88. 2005.... - Characterization of type II phosphatidylinositol 4-kinase isoforms reveals association of the enzymes with endosomal vesicular compartmentsAndras Balla
Endocrinology and Reproduction Research Branch, NICHD, and Laboratory of Host Defenses, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 277:20041-50. 2002..Our data indicate the existence of multiple forms of type II PI 4-kinase in mammalian cells and suggest that their functions are related to the endocytic pathway... - Pharmacology of phosphoinositides, regulators of multiple cellular functionsT Balla
Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Curr Pharm Des 7:475-507. 2001..This review briefly summarizes the means by which inositide functions have been pharmacologically manipulated, and discusses possibilities for specifically targeting certain aspects of their regulatory functions... - A PH domain in the Arf GTPase-activating protein (GAP) ARAP1 binds phosphatidylinositol 3,4,5-trisphosphate and regulates Arf GAP activity independently of recruitment to the plasma membranesFanny Campa
Laboratory of Cellular and Molecular Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 284:28069-83. 2009..In our model, ARAP1 is recruited to membranes independently of PtdIns(3,4,5)P(3), the subsequent production of which triggers enzymatic activity... - Phosphatidylinositol 4,5-bisphosphate and Arf6-regulated membrane trafficF D Brown
Laboratory of Cell Biology, National Heart Lung and Blood Institute, National Institute of Health, Bethesda, MD 20892, USA
J Cell Biol 154:1007-17. 2001..These results demonstrate that PIP 5-kinase activity and PIP2 turnover controlled by activation and inactivation of Arf6 is critical for trafficking through the Arf6 PM-endosomal recycling pathway... - Visualization of cellular phosphoinositide pools with GFP-fused protein-domainsTamas Balla
National Institutes of Health, Bethesda, Maryland, USA
Curr Protoc Cell Biol . 2009..This unit summarizes the design and practical use of these constructs and also reviews important considerations for interpretation of the data obtained by this technique... - Isolation and molecular cloning of wortmannin-sensitive bovine type III phosphatidylinositol 4-kinasesT Balla
Endocrinology and Reproduction Research Branch, NICHD, National Institutes of Health, Bethesda, Maryland 20892 4510, USA
J Biol Chem 272:18358-66. 1997..5-kb transcript. The molecular cloning of these novel WT-sensitive type III PI 4-kinases will allow detailed analysis of their signaling and other regulatory functions in mammalian cells... - Inhibition of Na,K-ATPase activates PI3 kinase and inhibits apoptosis in LLC-PK1 cellsX Zhou
Division of Nephrology, Uniformed Services University, Bethesda, Maryland 20814, USA
Biochem Biophys Res Commun 285:46-51. 2001..These data together indicate that inhibition of Na,K-ATPase activates PI3 kinase in LLC-PK1 cells which could then exert the cytoprotective effect... - A conserved NPLFY sequence contributes to agonist binding and signal transduction but is not an internalization signal for the type 1 angiotensin II receptorL Hunyady
Endocrinology and Reproduction Research Branch, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 270:16602-9. 1995..However, the Phe301 residue contributes significantly to agonist binding, and Asn298 is required for normal receptor activation and signal transduction... - Visualizing cellular phosphoinositide pools with GFP-fused protein-modulesTamas Balla
Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Sci STKE 2002:pl3. 2002..Here, we summarize our experience in designing and using these constructs and review our position concerning the interpretation of the data obtained by this technique... - Found in the crystal: phospholipid ligands for nuclear orphan receptorsTamas Balla
Endocrinology and Reproduction Research Branch, NICHD, National Institutes of Health, Bethesda, MD 20892 4510, USA
Trends Endocrinol Metab 16:289-90. 2005..Their ability to alter transcriptional activity by acting as bona fide ligands has been inspirational not only for the transcription factor community, but also for phospholipid researchers... - Phosphatidylinositol 4-kinase IIIbeta regulates the transport of ceramide between the endoplasmic reticulum and GolgiBalazs Toth
Section on Molecular Signal Transduction, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 281:36369-77. 2006..Therefore, PI 4-kinase beta is a key enzyme in the control of spingomyelin synthesis by controlling the flow of ceramide from the ER to the Golgi compartment... - Phosphatidylinositol 3-kinase-dependent membrane association of the Bruton's tyrosine kinase pleckstrin homology domain visualized in single living cellsP Varnai
Endocrinology and Reproduction Research Branch, NICHD, National Institutes of Health, Bethesda, Maryland 20892 4510, USA
J Biol Chem 274:10983-9. 1999.... - Visualization and manipulation of phosphoinositide dynamics in live cells using engineered protein domainsPeter Varnai
Endocrinology and Reproduction Research Branch, NICHD, National Institutes of Health, Bldg 49, Rm 6A35, 49 Convent Drive, Bethesda, MD, USA
Pflugers Arch 455:69-82. 2007..In this review, we will summarize our efforts to improve our tools in studying phosphoinositide dynamics and discuss our views on the values of these methods compared to other options currently used or being explored... - Phosphatidylinositol 4-kinases: old enzymes with emerging functionsAndras Balla
Endocrinology and Reproduction Research Branch, NICHD, National Institutes of Health, Bethesda, MD 20892, USA
Trends Cell Biol 16:351-61. 2006.... - Live cell imaging of phosphoinositide dynamics with fluorescent protein domainsPeter Varnai
Endocrinology and Reproduction Research Branch, NICHD, National Institutes of Health, Bethesda, MD 20892 4510, USA
Biochim Biophys Acta 1761:957-67. 2006..The most important value of these debates is that they also challenge our preconceived views of how phosphoinositides regulate cellular functions... - Differential PI 3-kinase dependence of early and late phases of recycling of the internalized AT1 angiotensin receptorLaszlo Hunyady
Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
J Cell Biol 157:1211-22. 2002.... - Store-operated Ca2+ influx and subplasmalemmal mitochondriaMarek K Korzeniowski
Section on Molecular Signal Transduction, Program on Developmental Neuroscience, NICHD, NIH, Bethesda, MD, USA
Cell Calcium 46:49-55. 2009..These observations indicate that mitochondria are exposed to Ca(2+) diffused laterally from the HCMDs formed between the PM and the subplasmalemmal ER... - Acute manipulation of Golgi phosphoinositides to assess their importance in cellular trafficking and signalingZsofia Szentpetery
Section on Molecular Signal Transduction, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 107:8225-30. 2010..This unique approach will allow further studies on the role of phosphoinositides in endocytic compartments that have evaded detection using the conventional long-term manipulations of inositide kinase and phosphatase activities... - The dynamics of plasma membrane PtdIns(4,5)P(2) at fertilization of mouse eggsGuillaume Halet
Department of Physiology, University College London, Gower Street, London WC1E 6BT, UK
J Cell Sci 115:2139-49. 2002..These studies suggest that fertilization does not deplete plasma membrane PtdIns(4,5)P(2) and that one of the pathways for increasing PtdIns(4,5)P(2) at fertilization is invoked by exocytosis of cortical granules... - A pharmacological map of the PI3-K family defines a role for p110alpha in insulin signalingZachary A Knight
Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, CA 94143, USA
Cell 125:733-47. 2006..These results illustrate systematic target validation using a matrix of inhibitors that span a protein family... - A membrane capture assay for lipid kinase activityZachary A Knight
Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, California 94158, USA
Nat Protoc 2:2459-66. 2007..We also describe a MATLAB script that automates the data analysis. The complete procedure requires 3-4 h... - Dual regulation of TRPV1 by phosphoinositidesViktor Lukacs
Department of Pharmacology and Physiology, University of Medicine and Dentistry of New Jersey New Jersey Medical School, Newark, New Jersey 07103, USA
J Neurosci 27:7070-80. 2007..We conclude that phosphoinositides have both inhibitory and activating effects on TRPV1, resulting in complex and distinct regulation at various stimulation levels... - Loss of endocytic clathrin-coated pits upon acute depletion of phosphatidylinositol 4,5-bisphosphateRoberto Zoncu
Department of Cell Biology and Howard Hughes Medical Institute, Kavli Institute For Neuroscience and Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT 06510, USA
Proc Natl Acad Sci U S A 104:3793-8. 2007..These findings demonstrate the critical importance of PI(4,5)P(2) in clathrin coat dynamics and Arp2/3-dependent actin regulation... - Regulation of connexin43 gap junctional communication by phosphatidylinositol 4,5-bisphosphateLeonie van Zeijl
Division of Cellular Biochemistry, Centre for Biomedical Genetics, The Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands
J Cell Biol 177:881-91. 2007.... - Phosphoinositide 3-kinase is required for intracellular Listeria monocytogenes actin-based motility and filopod formationGurjit Sidhu
Department of Medicine, Division of Infectious Diseases, University of Florida College of Medicine, Gainesville, Florida 32610, USA
J Biol Chem 280:11379-86. 2005.... - Control of cell polarity and motility by the PtdIns(3,4,5)P3 phosphatase SHIP1Miki Nishio
Department of Pathology and Immunology, Akita University School of Medicine, 1 1 1 Hondo, Akita 010 8543, Japan
Nat Cell Biol 9:36-44. 2007..By directing where PtdIns(3,4,5)P(3) accumulates, SHIP1 governs the formation of the leading edge and polarization required for chemotaxis... - c-Met must translocate to the nucleus to initiate calcium signalsDawidson A Gomes
Department of Internal Medicine and Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06520 8019, USA
J Biol Chem 283:4344-51. 2008..HGF may exert its particular effects on cells because it bypasses signaling pathways in the cytoplasm to directly activate signaling pathways in the nucleus...