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Genomes and Genes | Robert S BalabanSummaryAffiliation: National Institutes of Health Country: USA Publications
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Publications
Role of calcium in metabolic signaling between cardiac sarcoplasmic reticulum and mitochondria in vitroRobert S Balaban
Laboratory of Cardiac Energetics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1061, USA
Am J Physiol Cell Physiol 284:C285-93. 2003..It is suggested that this balanced activation by cytosolic Ca(2+) is partially responsible for the minimal alteration in energy metabolism intermediates that occurs with changes in cardiac workload in vivo...- Maintenance of the metabolic homeostasis of the heart: developing a systems analysis approachRobert S Balaban
Laboratory of Cardiac Energetics, National Heart Lung and Blood Institute, National Institute of Health, 9000 Rockville Pike, Bethesda MD 20892, USA
Ann N Y Acad Sci 1080:140-53. 2006..In addition, complex I is far from equilibrium and may play an important role in regulating the rate of reducing equivalent delivery to the cytochromes... 
The mitochondrial proteome: a dynamic functional program in tissues and disease statesRobert S Balaban
Laboratory of Cardiac Energetics, National Heart Lung and Blood Institute, Department of Health and Human Services, Bethesda, Maryland, USA
Environ Mol Mutagen 51:352-9. 2010..Screening methods are being introduced to detect the active or dynamic posttranslational sites to focus attention on sites that might provide insight into regulatory mechanisms...- Domestication of the cardiac mitochondrion for energy conversionRobert S Balaban
Laboratory of Cardiac Energetic, National Heart Lung and Blood Institute, Bethesda, MD 20892, USA
J Mol Cell Cardiol 46:832-41. 2009.... - Tissue heterogeneity of the mammalian mitochondrial proteomeD Thor Johnson
Laboratory of Cardiac Energetics, National Heart, Lung, and Blood Institute, National Institutes of Health, 10 Center Dr, Rm B1D416, Bethesda, MD 20892 1061, USA
Am J Physiol Cell Physiol 292:C689-97. 2007..These data confirm the notion that mitochondria are tuned by the nucleus for specific functions in different tissues... - The role of Ca(2+) signaling in the coordination of mitochondrial ATP production with cardiac workRobert S Balaban
Laboratory of Cardiac Energetics, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Biochim Biophys Acta 1787:1334-41. 2009..This balanced activation extends the energy homeostasis observed in the cytosol into the mitochondria matrix in the never resting heart... - Modeling mitochondrial functionRobert S Balaban
Laboratory of Cardiac Energetics, National Heart Lung and Blood Institute, Bethesda, MD 20892, USA
Am J Physiol Cell Physiol 291:C1107-13. 2006.... - Use of (32)P to study dynamics of the mitochondrial phosphoproteomeAngel M Aponte
Laboratory of Cardiac Energetics and Proteomics Core Facility, National Heart, Lung and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892 1061, USA
J Proteome Res 8:2679-95. 2009..These results demonstrate that a dynamic and extensive mitochondrial matrix phosphoproteome exists in heart and liver... - Regulation of oxidative phosphorylation complex activity: effects of tissue-specific metabolic stress within an allometric series and acute changes in workloadDarci Phillips
Laboratory of Cardiac Energetics, NHLBI, NIH, Bethesda, MD 20892 1061, USA
Am J Physiol Regul Integr Comp Physiol 302:R1034-48. 2012..These data are consistent with the notion that metabolic stress modulates MOPCs activity in the heart... - Quantitative mitochondrial phosphoproteomics using iTRAQ on an LTQ-Orbitrap with high energy collision dissociationEmily S Boja
Proteomics Core Facility, National Heart, Lung and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892 1061, USA
J Proteome Res 8:4665-75. 2009..This screening validated the iTRAQ/HCD technology as a method for functional quantitation of mitochondrial protein phosphorylation as well as providing insight into the regulation of mitochondria via phosphorylation... - The mammalian target of rapamycin (mTOR) pathway regulates mitochondrial oxygen consumption and oxidative capacityStefan M Schieke
Cardiology Branch, Laboratory of Cardiac Energetics, Flow Cytometry Core Facility, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 281:27643-52. 2006..These results may provide insight into recent observations linking the TOR pathway to life span regulation of lower organisms... - The mammalian longevity-associated gene product p66shc regulates mitochondrial metabolismShino Nemoto
Cardiology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bldg 10 6N 240, 10 Center Drive, Bethesda, MD 20892 1622, USA
J Biol Chem 281:10555-60. 2006..These results demonstrate that p66(shc) regulates mitochondrial oxidative capacity and suggest that p66(shc) may extend life span by repartitioning metabolic energy conversion away from oxidative and toward glycolytic pathways... - 32P labeling of protein phosphorylation and metabolite association in the mitochondria matrixAngel M Aponte
Proteomics Core Facility, National Heart Lung and Blood Institute, DHHS, Bethesda, Maryland, USA
Methods Enzymol 457:63-80. 2009..The use of this, in situ, labeling approach is extremely valuable in confirming protein phosphorylation sites as well as examining the dynamics of these processes under near physiological conditions... - Stoichiometry of STAT3 and mitochondrial proteins: Implications for the regulation of oxidative phosphorylation by protein-protein interactionsDarci Phillips
Laboratory of Cardiac Energetics, National Institutes of Health, Bethesda, MD 20892, USA
J Biol Chem 285:23532-6. 2010..Thus, STAT3 is likely altering mitochondrial function via transcriptional regulation or indirect signaling pathways that warrant further investigation... - Proteomic changes associated with diabetes in the BB-DP ratD Thor Johnson
National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1061, USA
Am J Physiol Endocrinol Metab 296:E422-32. 2009.... - Succinyl-CoA synthetase is a phosphate target for the activation of mitochondrial metabolismDarci Phillips
Laboratory of Cardiac Energetics, National Heart, Lung, and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892 1061, USA
Biochemistry 48:7140-9. 2009.... - Homogenous protein programming in the mammalian left and right ventricle free wallsDarci Phillips
Laboratory of Cardiac Energetics, National Heart, Lung, and Blood Institute, Bethesda, MD 20892 1061, USA
Physiol Genomics 43:1198-206. 2011..The constant myocyte composition in the LV and RV implies that the ratio of energy metabolism and contractile elements may be optimal for the sustained cardiac contractile activity in the mammalian heart... - Metabolic network control of oxidative phosphorylation: multiple roles of inorganic phosphateSalil Bose
Laboratory of Cardiac Energetics, NHLBI, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892, USA
J Biol Chem 278:39155-65. 2003.... - Distribution of mitochondrial NADH fluorescence lifetimes: steady-state kinetics of matrix NADH interactionsKsenia Blinova
Laboratory of Cardiac Energetics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Biochemistry 44:2585-94. 2005.... - p53 improves aerobic exercise capacity and augments skeletal muscle mitochondrial DNA contentJoon Young Park
Translational Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Circ Res 105:705-12, 11 p following 712. 2009..Understanding the biology of how p53 improves exercise capacity may provide useful insights for improving both cardiovascular as well as general health... - Mitochondrial matrix phosphoproteome: effect of extra mitochondrial calciumRachel K Hopper
Laboratory of Cardiac Energetics, National Heart, Lung and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892-1061, USA
Biochemistry 45:2524-36. 2006..These data demonstrate the use of a phosphoproteome screen in determining mitochondrial signaling pathways and reveal new pathways for Ca(2+) modification of mitochondrial function at the level of MnSOD... - Functional consequences of mitochondrial proteome heterogeneityD Thor Johnson
Laboratory of Cardiac Energetics, National Heart Lung and Blood Institute, National Institutes of Health, 10 Center Dr, Rm B1D416, Bethesda, MD 20892 1061, USA
Am J Physiol Cell Physiol 292:C698-707. 2007..These studies demonstrate the existence of mitochondrial biochemical functions that at present are poorly defined... - Continuous monitoring of enzymatic activity within native electrophoresis gels: application to mitochondrial oxidative phosphorylation complexesRaul Covian
Laboratory of Cardiac Energetics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1061, USA
Anal Biochem 431:30-9. 2012..The utility of monitoring the entire kinetic behavior of these reactions in native gels, as well as the general application of this approach, is discussed... - Intrinsic protein kinase activity in mitochondrial oxidative phosphorylation complexesDarci Phillips
Laboratory of Cardiac Energetics, National Heart, Lung and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892, United States
Biochemistry 50:2515-29. 2011..Collectively, this study proposes that many of the mitochondrial complexes contain an autophosphorylation mechanism, which may play a functional role in the regulation of these multiprotein units... - Short communication: Subcellular motion compensation for minimally invasive microscopy, in vivo: evidence for oxygen gradients in resting muscleJames L Schroeder
Laboratory of Cardiac Energetics, National Heart, Lung, and Blood Institute, NIH, 10 Center Drive, Bethesda, MD 20892, USA
Circ Res 106:1129-33. 2010..A primary limitation of optical microscopy in vivo is tissue motion, which prevents physiological time course observations or image averaging... - Cardiac mitochondrial matrix and respiratory complex protein phosphorylationRaul Covian
Laboratory of Cardiac Energetics, National Heart Lung and Blood Institute, Bethesda, Maryland 20817, USA
Am J Physiol Heart Circ Physiol 303:H940-66. 2012.... - Role of mitochondrial Ca2+ in the regulation of cellular energeticsBrian Glancy
Laboratory of Cardiac Energetics, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20817, USA
Biochemistry 51:2959-73. 2012..Though most specific molecular mechanisms have yet to be elucidated, it is clear that Ca(2+) provides a balanced activation of mitochondrial energy metabolism that exceeds the alteration of dehydrogenases alone... - Stunned, infarcted, and normal myocardium in dogs: simultaneous differentiation by using gadolinium-enhanced cine MR imaging with magnetization transfer contrastClifford R Weiss
Laboratory of Cardiac Energetics, National Heart, Lung, and Blood Institute, National Institutes of Health, Department of Health and Human Services, 10 Center Dr, Bldg 10, Rm B1D416, MSC 1061, Bethesda, MD 20892-1061, USA
Radiology 226:723-30. 2003..CONCLUSION: One day after myocardial ischemia, MTET during one MR imaging examination enabled simultaneous differentiation of infarcted, stunned, and normal myocardial regions on the basis of gadolinium enhancement and regional function... - Absolute myocardial perfusion in canines measured by using dual-bolus first-pass MR imagingTimothy F Christian
Laboratory of Cardiac Energetics, National Heart, Lung and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bldg 10, Rm B1D416, MSC 1061, 10 Center Dr, Bethesda, MD 20892-1061, USA
Radiology 232:677-84. 2004..0 mL/min/g). Use of qualitative MR imaging measures such as the contrast enhancement ratio led to substantially underestimated hyperemic blood flow measurements... - Mitochondrial NADH fluorescence is enhanced by complex I bindingKsenia Blinova
Laboratory of Cardiac Energetics, National Heart Lung and Blood Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, Maryland 20892, USA
Biochemistry 47:9636-45. 2008.... - NADH enzyme-dependent fluorescence recovery after photobleaching (ED-FRAP): applications to enzyme and mitochondrial reaction kinetics, in vitroFrederic Joubert
Laboratory of Cardiac Energetics, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892-1061, USA
Biophys J 86:629-45. 2004..Initial data support the notion that the NADH regeneration process is far from equilibrium in mitochondria and is potentially controlled by NADH levels as well as several other matrix factors... - Measurement of skeletal muscle perfusion during postischemic reactive hyperemia using contrast-enhanced MRI with a step-input functionRichard B Thompson
Laboratory of Cardiac Energetics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
Magn Reson Med 54:289-98. 2005..4% from tissue relaxometry studies. Bulk blood flow studies in the same volunteers using phase-contrast velocimetry (popliteal artery) yielded significantly lower flow values, but with a correlation coefficient R2 = 0.62 and P = 0.004... - NAD(P)H fluorescence imaging of mitochondrial metabolism in contracting Xenopus skeletal muscle fibers: effect of oxygen availabilityMichael C Hogan
NHLBI Light Microscopy Facility, National Institutes of Health, 9000 Rockville Pike, Bldg. 10/Room B1D-416, Bethesda, MD 20892-1061, USA
J Appl Physiol 98:1420-6. 2005..These results also demonstrate that although oxygen availability influences the rate of NAD(P)H oxidation, it does not prevent NAD(P)H from being oxidized through the process of oxidative phosphorylation at the onset of contractions... - Cardiac energy metabolism homeostasis: role of cytosolic calciumRobert S Balaban
Laboratory of Cardiac Energetics, National Heart Lung and Blood Institute NIH, Building 10, Room B1 D161, Bethesda, MD 20892, USA
J Mol Cell Cardiol 34:1259-71. 2002..A model of the cardiac energy metabolism control network is proposed that includes a Ca(2+) parallel activation component together with more classical elements including metabolite feedback and cytosolic compartmentation... - The visceral pericardium: macromolecular structure and contribution to passive mechanical properties of the left ventriclePaul D Jobsis
National Heart, Lung, and Blood Institute, National Institutes of Health, MSC 1061, 9000 Rockville Pike, Bethesda, MD 20892, USA
Am J Physiol Heart Circ Physiol 293:H3379-87. 2007..Alterations in this layer may occur in various disease states that effect diastolic function... 
Increased oxidative metabolism in the Li-Fraumeni syndromePing Yuan Wang
National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
N Engl J Med 368:1027-32. 2013..These results suggest that p53 regulates bioenergetic homeostasis in humans. (Funded by the National Heart, Lung, and Blood Institute and the National Institutes of Health; ClinicalTrials.gov number, NCT00406445.)...- In vivo study of microcirculation in canine myocardium using the IVIM methodVirginie Callot
Laboratory of Cardiac Energetics, NHLBI, NIH, Bethesda, Maryland 20892, USA
Magn Reson Med 50:531-40. 2003..With vasodilatation by adenosine infusion, a 25% increase in the VVF and a 7% increase in the mean microflow velocity were observed, while no change in the ADC was detected. A 28.5% decrease of the ADC was observed postmortem... - Contribution of mitochondria to cardiac muscle water/macromolecule proton magnetization transferKathleen Ward
Laboratory of Cardiac Energetics, National Heart Lung and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20817, USA
Magn Reson Med 50:1312-6. 2003..The current data indicate that mitochondria macromolecules contribute significantly to MT in the intact heart... - Enzyme-dependent fluorescence recovery after photobleaching of NADH: in vivo and in vitro applications to the study of enzyme kineticsChristian A Combs
Light Microscopy Facility, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Methods Enzymol 385:257-86. 2004 - The renal artery ostium flow diverter: structure and potential role in atherosclerosisEdward B Neufeld
Laboratory of Cardiac Energetics, NHLBI, NIH, Bethesda, MD 20892, United States
Atherosclerosis 211:153-8. 2010..We propose that the rigid macromolecular structure of the renal artery ostium diverter is required for its vascular function and contributes to the initiation of renal atherosclerosis by the retention of LDL... - Skeletal muscle NAD(P)H two-photon fluorescence microscopy in vivo: topology and optical inner filtersEmily C Rothstein
Laboratory of Cardiac Energetics, National Heart, Lung and Blood Institute, National Institutes of Health, Department of Human Health Services, Bldg 10, Rm B1D416, 9000 Rockville Pike, Bethesda, MD 20892, USA
Biophys J 88:2165-76. 2005..These data demonstrate the feasibility, and highlight the complexity, of using NAD(P)H TPEFM in skeletal muscle to characterize the topology and metabolic function of mitochondria within the living mouse... - Multi-photon excitation microscopy in intact animalsEmily C Rothstein
Laboratory of Cardiac Energetics, National Heart, Lung and Blood Institute, National Institutes of Health, Department of Human Health Services, Bethesda, MD 20892, USA
J Microsc 222:58-64. 2006..The remaining most significant issue for physiological studies using this approach is motion on the micrometre scale. Several strategies for motion compensation are described and discussed... - Limited utility of acetoxymethyl (AM)-based intracellular delivery systems, in vivo: interference by extracellular esterasesPaul D Jobsis
Laboratory of Cardiac Energetics, National Heart Lung and Blood Institute, Bethesda, Maryland 20892, USA
J Microsc 226:74-81. 2007..Different approaches to trapping exogenous probes will need to be explored for physiological studies using intravital microscopy... - Automatic assessment of dynamic contrast-enhanced MRI in an ischemic rat hindlimb model: an exploratory study of transplanted multipotent progenitor cellsLi Yueh Hsu
National Heart Lung and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892 1061, USA
NMR Biomed 21:111-9. 2008..The differences were primarily determined by late contrast enhancement of PBS-treated limbs. These computerized methods appear promising for assessing perfusion and late enhancement in dynamic contrast-enhanced MRI... - Protein composition and function of red and white skeletal muscle mitochondriaBrian Glancy
National Heart, Lung, and Blood Institute NIH, 10 Center Drive, Bethesda, MD 20892, USA
Am J Physiol Cell Physiol 300:C1280-90. 2011..These data suggest that metabolic demand differences between red and white muscle fibers are primarily matched by the number of mitochondria and not by significant alterations in the mitochondria themselves... - A discordance in rosiglitazone mediated insulin sensitization and skeletal muscle mitochondrial content/activity in Type 2 diabetes mellitusInes Pagel Langenickel
Cardiology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1454, USA
Am J Physiol Heart Circ Physiol 293:H2659-66. 2007..It remains to be determined whether longer-term insulin sensitization therapy with rosiglitazone will augment skeletal muscle mitochondrial bioenergetics in those diabetic subjects with relatively preserved basal aerobic capacity... - Mitochondria, oxidants, and agingRobert S Balaban
Laboratory of Cardiac Energetics, National Institutes of Health, Bethesda, Maryland 20892, USA
Cell 120:483-95. 2005..Here we review the evidence that both supports and conflicts with the free radical theory and examine the growing link between mitochondrial metabolism, oxidant formation, and the biology of aging... - Does binding of Gd-DTPA to myocardial tissue contribute to late enhancement in a model of acute myocardial infarction?Ulrich K M Decking
Laboratory of Cardiac Energetics, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
Magn Reson Med 49:168-71. 2003..The data from this acute myocardial infarction model do not support the notion that Gd-DTPA binding in the early stages of myocardial damage contributes to delayed enhancement... - Detecting acute coronary syndrome in the emergency department with cardiac magnetic resonance imagingRaymond Y Kwong
Laboratory of Cardiac Energetics, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892-1061, USA
Circulation 107:531-7. 2003..Performed urgently to evaluate chest pain, MRI accurately detected a high fraction of patients with acute coronary syndrome, including patients with enzyme-negative unstable angina... - Highly efficient endosomal labeling of progenitor and stem cells with large magnetic particles allows magnetic resonance imaging of single cellsKathleen A Hinds
Hematology Branch, Laboratory of Molecular Biology, Laboratory National Heart, Lung, and Blood Institute (NHLBI, National Institutes of Health, Bethesda, MD 20892, USA
Blood 102:867-72. 2003..MRI studies could detect labeled CD34+ cells and mesenchymal stem cells (MSCs) at single cell resolution. This appears to be a promising tool for serial noninvasive monitoring of in vivo cell homing and localization using MRI... - A functional genomic screen for cardiogenic genes using RNA interference in developing Drosophila embryosYong-Ou Kim
Laboratory Research Program, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 101:159-64. 2004..Together, these studies not only identify key regulators but also reveal mechanisms underlying heart development... - Multislice first-pass cardiac perfusion MRI: validation in a model of myocardial infarctionFrederick H Epstein
Laboratory of Cardiac Energetics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
Magn Reson Med 47:482-91. 2002..Additionally, analysis of myocardial time-intensity curves (TICs) indicated that 15.8 +/- 6 sectors, corresponding to 260 microl of endocardium, can be analyzed (R(2) > 0.95)... - Fluorescence absorbance inner-filter decomposition: the role of emission shape on estimates of free Ca(2+) using Rhod-2Paul R Territo
Lilly Center for Molecular and Anatomical Imaging, Lilly Research Laboratories, Eli Lilly and Company, 2001 West Main Street, B220 GL54, Greenfield, Indiana 46140, USA
Appl Spectrosc 61:138-47. 2007..These data demonstrate that secondary inner-filter correction can significantly improve spectral decomposition of complex emission spectra, which are used in a variety of biological applications... - Hypoxia-induced left ventricular dysfunction in myoglobin-deficient micePradeep P A Mammen
Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 8573, USA
Am J Physiol Heart Circ Physiol 285:H2132-41. 2003..These new data establish that myoglobin is an important cytoplasmic cardiac hemoprotein that functions in regulating NO homeostasis within cardiomyocytes... - High-resolution imaging reveals a limit in spatial resolution of blood flow measurements by microspheresUlrich K M Decking
Department of Cardiovascular Physiology, Heinrich Heine University, 40225 Dusseldorf, Germany
Am J Physiol Heart Circ Physiol 287:H1132-40. 2004..We propose that at high spatial resolution (<2 microl) structural aspects of the vascular network dominate microsphere distribution, resulting in the organized patterns observed... - Contribution of macromolecular structure to the retention of low-density lipoprotein at arterial branch pointsGina P Kwon
Hughes Medical Institute, Chevy Chase, MD, USA
Circulation 117:2919-27. 2008..In this study, we characterized the submicron microstructure of arterial wall collagen and elastin to evaluate its potential role in regional LDL deposition...