Donald H Atha

Summary

Affiliation: National Institute of Standards and Technology
Country: USA

Publications

  1. doi request reprint High-throughput DNA diagnostic measurements using capillary electrophoresis: p53, fragile X and telomerase
    Donald H Atha
    National Institute of Standards and Technology, Biochemical Science Division, 100 Bureau Drive, MS 8311, Gaithersburg, MD 20899, USA 1 301 975 3092 1 301 975 8505
    Expert Opin Med Diagn 2:91-100. 2008
  2. pmc Standards for immunohistochemical imaging: a protein reference device for biomarker quantitation
    Donald H Atha
    Biochemical Science Division, National Institute of Standards and Technology, bldg 227, Room A243, Mail Stop 8311, 100 Bureau Drive, Gaithersburg, MD 20899 8311, USA
    J Histochem Cytochem 58:1005-14. 2010
  3. ncbi request reprint Detection of p53 point mutations by single strand conformation polymorphism: analysis by capillary electrophoresis
    D H Atha
    Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Electrophoresis 19:172-9. 1998
  4. ncbi request reprint High-throughput analysis of telomerase by capillary electrophoresis
    Donald H Atha
    Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 8311, USA
    Electrophoresis 24:109-14. 2003
  5. ncbi request reprint Characterization of DNA standards by capillary electrophoresis
    D H Atha
    Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Electrophoresis 19:1428-35. 1998
  6. pmc Prediction of DNA single-strand conformation polymorphism: analysis by capillary electrophoresis and computerized DNA modeling
    D H Atha
    Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Nucleic Acids Res 29:4643-53. 2001
  7. ncbi request reprint Measurement of DNA biomarkers for the safety of tissue-engineered medical products, using artificial skin as a model
    Henry Rodriguez
    Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Tissue Eng 10:1332-45. 2004
  8. ncbi request reprint Preparation and characterization of candidate reference materials for telomerase assays
    John P Jakupciak
    Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Clin Chem 51:1443-50. 2005
  9. ncbi request reprint Renewable standard reference material for the detection of TP53 mutations
    Catherine D O'Connell
    Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    Mol Diagn 7:85-97. 2003
  10. pmc Analytical validation of telomerase activity for cancer early detection: TRAP/PCR-CE and hTERT mRNA quantification assay for high-throughput screening of tumor cells
    John P Jakupciak
    NIST, Biotechnology Division, 100 Bureau Drive, MS 8311, Gaithersburg, MD 20899, USA
    J Mol Diagn 6:157-65. 2004

Collaborators

Detail Information

Publications18

  1. doi request reprint High-throughput DNA diagnostic measurements using capillary electrophoresis: p53, fragile X and telomerase
    Donald H Atha
    National Institute of Standards and Technology, Biochemical Science Division, 100 Bureau Drive, MS 8311, Gaithersburg, MD 20899, USA 1 301 975 3092 1 301 975 8505
    Expert Opin Med Diagn 2:91-100. 2008
    ..In this regard, the examples given in this review help to demonstrate that CE can replace previous slab-gel methods and show that CE can improve a wide range of applications in the medical field...
  2. pmc Standards for immunohistochemical imaging: a protein reference device for biomarker quantitation
    Donald H Atha
    Biochemical Science Division, National Institute of Standards and Technology, bldg 227, Room A243, Mail Stop 8311, 100 Bureau Drive, Gaithersburg, MD 20899 8311, USA
    J Histochem Cytochem 58:1005-14. 2010
    ..Further development will be required to establish accurate surface protein concentrations in the range required for specific clinical applications...
  3. ncbi request reprint Detection of p53 point mutations by single strand conformation polymorphism: analysis by capillary electrophoresis
    D H Atha
    Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Electrophoresis 19:172-9. 1998
    ..The combination of mutation-specific temperature profiling and analysis of isoforms by CE-SSCP should be of help to the diagnostic community in the detection of genetic mutations...
  4. ncbi request reprint High-throughput analysis of telomerase by capillary electrophoresis
    Donald H Atha
    Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 8311, USA
    Electrophoresis 24:109-14. 2003
    ..With the appropriate robotic sample handling system, these CE methods would enable performing the telomerase TRAP assay with increased sensitivity, reproducibility and automation over slab-gel methods...
  5. ncbi request reprint Characterization of DNA standards by capillary electrophoresis
    D H Atha
    Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Electrophoresis 19:1428-35. 1998
    ..These measurements yield preliminary information useful in evaluating DNA size standards which may be used for a wide range of DNA diagnostic applications...
  6. pmc Prediction of DNA single-strand conformation polymorphism: analysis by capillary electrophoresis and computerized DNA modeling
    D H Atha
    Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Nucleic Acids Res 29:4643-53. 2001
    ..The results may also impact other diagnostic methods, which would benefit by a better understanding of DNA single-strand conformation polymorphisms to optimize conditions for enzymatic cleavage and DNA hybridization reactions...
  7. ncbi request reprint Measurement of DNA biomarkers for the safety of tissue-engineered medical products, using artificial skin as a model
    Henry Rodriguez
    Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Tissue Eng 10:1332-45. 2004
    ..More importantly, this exploratory work addresses technological considerations that need to be addressed in order to expedite accurate and useful international reference standards for the emerging tissue-engineering industry...
  8. ncbi request reprint Preparation and characterization of candidate reference materials for telomerase assays
    John P Jakupciak
    Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Clin Chem 51:1443-50. 2005
    ....
  9. ncbi request reprint Renewable standard reference material for the detection of TP53 mutations
    Catherine D O'Connell
    Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    Mol Diagn 7:85-97. 2003
    ..Their use will improve disease detection by serving as validation materials to monitor errors in measurement methods, including PCR amplification, amplicon separation, and data analysis from different technology platforms...
  10. pmc Analytical validation of telomerase activity for cancer early detection: TRAP/PCR-CE and hTERT mRNA quantification assay for high-throughput screening of tumor cells
    John P Jakupciak
    NIST, Biotechnology Division, 100 Bureau Drive, MS 8311, Gaithersburg, MD 20899, USA
    J Mol Diagn 6:157-65. 2004
    ..Using this combination of telomerase activity and hTERT mRNA measurements, the automated system improved efficiency over traditional TRAP/PCR methods...
  11. ncbi request reprint Standards for validation of cancer biomarkers
    Catherine D O'Connell
    Tetracore, Inc, Gaithersburg, MD 20878, USA
    Cancer Biomark 1:233-9. 2005
    ....
  12. ncbi request reprint Real-time telomerase assay of less-invasively collected esophageal cell samples
    Brenna M McGruder
    Biochemical Science Division, National Institute of Standards and Technology, 100 Bureau Drive, MS 8311, 20899, Gaithersburg, MD, USA
    Cancer Lett 244:91-100. 2006
    ..These findings demonstrate the feasibility of using the RTTRAP assay in EBC samples and suggest that individuals from high-risk populations can be screened for telomerase activity...
  13. doi request reprint NIST gold nanoparticle reference materials do not induce oxidative DNA damage
    Bryant C Nelson
    National Institute of Standards and Technology, Material Measurement Laboratory, Gaithersburg, MD 20899, USA
    Nanotoxicology 7:21-9. 2013
    ..NIST AuNPs thus hold substantial promise for improving the reproducibility and reliability of nanoparticle genotoxicity studies...
  14. doi request reprint Copper oxide nanoparticle mediated DNA damage in terrestrial plant models
    Donald H Atha
    Material Measurement Laboratory, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, Maryland 20899, United States
    Environ Sci Technol 46:1819-27. 2012
    ..To our knowledge, this is the first evidence of multiple DNA lesion formation and accumulation in plants. These findings provide impetus for future investigations on nanoparticle-mediated DNA damage and repair mechanisms in plants...
  15. pmc Conformational analysis of the telomerase RNA pseudoknot hairpin by Raman spectroscopy
    Vytas Reipa
    Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899 8311, USA
    RNA 13:108-15. 2007
    ..This demonstrates that Raman bands, previously assigned to specific bases or conformational RNA markers, can be used to probe local structural features of the telomerase pseudoknot hairpin sequence...
  16. ncbi request reprint Biomarkers used to detect genetic damage in tissue engineered skin
    Catherine O'Connell
    DNA Technologies Group, Chemical Science and Technology Laboratory, National Institute of Standards and Technology, 100 Bureau Drive, Mail Stop 831, Gaithersburg, MD 20899 8311, USA
    Adv Exp Med Biol 534:137-45. 2003
  17. ncbi request reprint Use of TP53 reference materials to validate mutations in clinical tissue specimens by single-strand conformational polymorphism analysis
    Bulbin Sunar-Reeder
    Department of Medical Biology and Genetics, Faculty of Medicine, Inonu University, Malatya, Turkey
    Mol Diagn 8:123-30. 2004
    ..A panel of 12 plasmid clones containing wild-type or point mutations within exons 5-9 have been developed as reference materials for the detection of TP53 mutations...
  18. ncbi request reprint Telomerase activity measurement in magnetically captured epithelial cells: comparison of slab-gel and capillary electrophoresis
    Jennifer L Hess
    Graduate Program in Human Genetics, University of Maryland School of Medicine, Baltimore, MD, USA
    Electrophoresis 25:1852-9. 2004
    ..A high-throughput CE-LIF detection platform will be indispensable for validation studies of telomerase activity measurements...