Research Topics
Genomes and GenesSpecies
| L AravindSummaryAffiliation: National Institutes of Health Country: USA Publications
| Collaborators
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Detail Information
Publications
The prokaryotic antecedents of the ubiquitin-signaling system and the early evolution of ubiquitin-like beta-grasp domainsLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
Genome Biol 7:R60. 2006..Several of these Ub-like proteins and the associated protein families are likely to function together in signaling systems just as in eukaryotes...
MutL homologs in restriction-modification systems and the origin of eukaryotic MORC ATPasesLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Biol Direct 3:8. 2008....
Comparative genomics and structural biology of the molecular innovations of eukaryotesL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Curr Opin Struct Biol 16:409-19. 2006....
Novel conserved domains in proteins with predicted roles in eukaryotic cell-cycle regulation, decapping and RNA stabilityVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
BMC Genomics 5:45. 2004..The identification of these RNA-binding domains may throw light on the emergence of eukaryote-specific systems of RNA metabolism...
Origin and evolution of the archaeo-eukaryotic primase superfamily and related palm-domain proteins: structural insights and new membersLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health Bethesda, MD 20894, USA
Nucleic Acids Res 33:3875-96. 2005..These observations point to a functional equivalence of the two classes of primases, which seem to have repeatedly displaced each other in various extrachromosomal replicons...
Regulatory potential, phyletic distribution and evolution of ancient, intracellular small-molecule-binding domainsV Anantharaman
National Center for Biotechnology Information National Library of Medicine, National Institutes of Health, Bethesda, MD, 20894, USA
J Mol Biol 307:1271-92. 2001..Using the "guilt by association approach", the identification of SMBDs allowed prediction of functions and mode of regulation for a variety of previously uncharacterized proteins...
Comparative genomics of the FtsK-HerA superfamily of pumping ATPases: implications for the origins of chromosome segregation, cell division and viral capsid packagingLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Nucleic Acids Res 32:5260-79. 2004....
The alpha/beta fold uracil DNA glycosylases: a common origin with diverse fatesL Aravind
National Center for Biotechnology Information, National Institutes of Health, Bethesda, MD 20894, USA
Genome Biol 1:RESEARCH0007. 2000..The structural and evolutionary relationships between the rest of the UDGs remain uncertain...
The SWIRM domain: a conserved module found in chromosomal proteins points to novel chromatin-modifying activitiesL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Genome Biol 3:RESEARCH0039. 2002..These modifications have an important role in the regulation of chromatin structure and are mediated by large multisubunit complexes that contain modular proteins with several conserved catalytic and noncatalytic adaptor domains...
A novel family of P-loop NTPases with an unusual phyletic distribution and transmembrane segments inserted within the NTPase domainL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Genome Biol 5:R30. 2004..These studies provide a framework for characterization of novel lineages within this fold and prediction of their functional properties...
The natural history of the WRKY-GCM1 zinc fingers and the relationship between transcription factors and transposonsM Madan Babu
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Nucleic Acids Res 34:6505-20. 2006..We also use the WRKY-GCM1 superfamily as an example to illustrate the importance of transposons in the emergence of new TFs in different lineages...
Comprehensive analysis of combinatorial regulation using the transcriptional regulatory network of yeastS Balaji
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda 20894, USA
J Mol Biol 360:213-27. 2006..We believe that the results presented here might provide a framework for testing the role of co-regulatory associations in eukaryotic transcriptional control...
The DNA-repair protein AlkB, EGL-9, and leprecan define new families of 2-oxoglutarate- and iron-dependent dioxygenasesL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Genome Biol 2:RESEARCH0007. 2001....
Quoderat demonstrandum? The mystery of experimental validation of apparently erroneous computational analyses of protein sequencesL M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Genome Biol 2:RESEARCH0051. 2001..Computational predictions are critical for directing the experimental study of protein functions. Therefore it is paradoxical when an apparently erroneous computational prediction seems to be supported by experiment...
Prokaryotic homologs of the eukaryotic DNA-end-binding protein Ku, novel domains in the Ku protein and prediction of a prokaryotic double-strand break repair systemL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
Genome Res 11:1365-74. 2001..A hypothesis is proposed that the Ku homolog, together with the associated gene products, comprise a previously unrecognized prokaryotic system for repair of double-strand breaks in DNA...
Classification of the caspase-hemoglobinase fold: detection of new families and implications for the origin of the eukaryotic separinsL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland, USA
Proteins 46:355-67. 2002..The acquisition of the caspase-hemoglobinase-fold domains by eukaryotes might have been critical in the evolution of important eukaryotic processes, such as mitosis and programmed cell death...
Origin and evolution of peptide-modifying dioxygenases and identification of the wybutosine hydroxylase/hydroperoxidaseLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Nucleic Acids Res 38:5261-79. 2010..The specificity of wybutosine hydroxylase/peroxidase probably relates to the structural similarity of the modified moiety to the ancestral amino acid substrate of this superfamily...
Polymorphic toxin systems: Comprehensive characterization of trafficking modes, processing, mechanisms of action, immunity and ecology using comparative genomicsDapeng Zhang
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Biol Direct 7:18. 2012..Prior analysis revealed a striking diversity of nuclease and deaminase domains among the toxin modules. We systematically investigated polymorphic toxin systems using comparative genomics, sequence and structure analysis...
Evolution of bacterial RNA polymerase: implications for large-scale bacterial phylogeny, domain accretion, and horizontal gene transferLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Gene 335:73-88. 2004....
Eukaryotic signalling domain homologues in archaea and bacteria. Ancient ancestry and horizontal gene transferC P Ponting
National Center for Biotechnology Information National Library of Medicine, National Institutes of Health, Bldg 38A, Bethesda, MD, 20894, USA
J Mol Biol 289:729-45. 1999..A number of transfers of signalling domains from eukaryotes to bacteria were confidently identified, in contrast to only a single case of apparent transfer from eukaryotes to archaea...
A novel immunity system for bacterial nucleic acid degrading toxins and its recruitment in various eukaryotic and DNA viral systemsDapeng Zhang
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Nucleic Acids Res 39:4532-52. 2011....
SWIM, a novel Zn-chelating domain present in bacteria, archaea and eukaryotesKira S Makarova
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Trends Biochem Sci 27:384-6. 2002..This domain was designated SWIM after SWI2/SNF2 and MuDR, and is predicted to have DNA-binding and protein-protein interaction functions in different contexts...
IMPALA: matching a protein sequence against a collection of PSI-BLAST-constructed position-specific score matricesA A Schaffer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Bioinformatics 15:1000-11. 1999..Also, it is considerably faster when run with a large database of PSSMs than is BLAST or PSI-BLAST when run against the complete non-redundant protein database...
Prediction of the archaeal exosome and its connections with the proteasome and the translation and transcription machineries by a comparative-genomic approachE V Koonin
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
Genome Res 11:240-52. 2001....
Diversification of catalytic activities and ligand interactions in the protein fold shared by the sugar isomerases, eIF2B, DeoR transcription factors, acyl-CoA transferases and methenyltetrahydrofolate synthetaseVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
J Mol Biol 356:823-42. 2006....
Genome of the extremely radiation-resistant bacterium Deinococcus radiodurans viewed from the perspective of comparative genomicsK S Makarova
Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799, USA
Microbiol Mol Biol Rev 65:44-79. 2001..In combination, these observations suggest that several different biological mechanisms contribute to the multiple DNA repair-dependent phenotypes of this organism...
TRAM, a predicted RNA-binding domain, common to tRNA uracil methylation and adenine thiolation enzymesV Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
FEMS Microbiol Lett 197:215-21. 2001..In addition, sequence analysis of the MiaB family enzymes showed that they share the predicted catalytic site with biotin and lipoate synthases and probably employ the same mechanism for sulfur insertion into their respective substrate...
Detection of novel members, structure-function analysis and evolutionary classification of the 2H phosphoesterase superfamilyRaja Mazumder
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Nucleic Acids Res 30:5229-43. 2002..Initially, this domain appears to have been involved in RNA processing and it appears to have been recruited to perform various other functions in later stages of evolution...
Evolutionary connection between the catalytic subunits of DNA-dependent RNA polymerases and eukaryotic RNA-dependent RNA polymerases and the origin of RNA polymerasesLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
BMC Struct Biol 3:1. 2003..No evolutionary relationship between RDRP and other polymerases has been reported so far, hence the origin of this eukaryote-specific polymerase remains a mystery...
The RAGNYA fold: a novel fold with multiple topological variants found in functionally diverse nucleic acid, nucleotide and peptide-binding proteinsS Balaji
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Nucleic Acids Res 35:5658-71. 2007..3) We also present evidence for a bacterial origin of the GYF domain and propose how this version of the fold might have been utilized in peptide interactions in the context of nucleoprotein complexes...
Apoptotic molecular machinery: vastly increased complexity in vertebrates revealed by genome comparisonsL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Science 291:1279-84. 2001..A considerable repertoire of apoptotic protein domains was detected in Actinomycetes and Cyanobacteria, which suggests a major contribution of horizontal gene transfer to the early evolution of apoptosis...
Adaptations of the helix-grip fold for ligand binding and catalysis in the START domain superfamilyL M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Proteins 43:134-44. 2001..Thus, the START domain superfamily is a rare case of the adaptation of a protein fold with a conserved ligand-binding mode for both a broad variety of catalytic activities and noncatalytic regulatory functions. Proteins 2001;43:134-144...
The role of lineage-specific gene family expansion in the evolution of eukaryotesOlivier Lespinet
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
Genome Res 12:1048-59. 2002..LSEs seem to be one of the principal means of adaptation and one of the most important sources of organizational and regulatory diversity in crown-group eukaryotes...
The two faces of Alba: the evolutionary connection between proteins participating in chromatin structure and RNA metabolismL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Genome Biol 4:R64. 2003..Here we use comparative genomics and sequence profile analysis to predict potential alternative functions of the Alba proteins...
Eukaryote-specific domains in translation initiation factors: implications for translation regulation and evolution of the translation systemL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institute of Health, Bethesda, MD 20894, USA
Genome Res 10:1172-84. 2000....
The emergence of catalytic and structural diversity within the beta-clip foldLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
Proteins 55:977-91. 2004..We also describe how assembly of this fold from a basic three-stranded unit has been utilized to accommodate a variety of activities in its different versions...
The ASCH superfamily: novel domains with a fold related to the PUA domain and a potential role in RNA metabolismLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Bioinformatics 22:257-63. 2006....
Application of comparative genomics in the identification and analysis of novel families of membrane-associated receptors in bacteriaVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
BMC Genomics 4:34. 2003..So far, they are relatively rare in the prokaryotes, and are restricted to the well-characterized sensory rhodopsins of various phototropic prokaryotes...
Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR-Cas systemsKira S Makarova
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, 8600 Rockville Pike, Bethesda, MD 20894, USA
Biol Direct 6:38. 2011..Comparative analysis of the Cas protein sequences and structures led to the classification of the CRISPR-Cas systems into three Types (I, II and III)...
HutC/FarR-like bacterial transcription factors of the GntR family contain a small molecule-binding domain of the chorismate lyase foldL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
FEMS Microbiol Lett 222:17-23. 2003..An ancestral, generic small-molecule-binding domain appears to have given rise to the enzymatic and non-catalytic ligand-binding versions of the same fold under the influence of different selective pressures...
A DNA repair system specific for thermophilic Archaea and bacteria predicted by genomic context analysisKira S Makarova
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Building 380, Bethesda, MD 20894, USA
Nucleic Acids Res 30:482-96. 2002....
Evolutionary genomics of nucleo-cytoplasmic large DNA virusesLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Virus Res 117:156-84. 2006....
Gene duplication with displacement and rearrangement: origin of the bacterial replication protein PriB from the single-stranded DNA-binding protein SsbVladimir A Ponomarev
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA
J Mol Microbiol Biotechnol 5:225-9. 2003....
MEDS and PocR are novel domains with a predicted role in sensing simple hydrocarbon derivatives in prokaryotic signal transduction systemsVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Bioinformatics 21:2805-11. 2005..Hence it is possible that these sigma factors, involved in aerial mycelium development and stress response in the actinomycetes, might be under the regulation of as yet uncharacterized small molecules. CONTACT: ...
CYSTM, a novel cysteine-rich transmembrane module with a role in stress tolerance across eukaryotesThiago M Venancio
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Bioinformatics 26:149-52. 2010..Thus, CYSTM proteins are likely to be part of a novel cellular protective mechanism that is widely active in eukaryotes, including humans. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online...
Pathogenic roles of CD14, galectin-3, and OX40 during experimental cerebral malaria in miceMiranda S Oakley
Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics and Evaluation Research, Food and Drug Administration, Bethesda, MD, USA
PLoS ONE 4:e6793. 2009..We find that OX40, a marker of both T cell activation and memory, is selectively upregulated in the brain during ECM and its distribution among CD4(+) and CD8(+) T cells accumulated in the brain vasculature is approximately equal...
Uncovering a hidden distributed architecture behind scale-free transcriptional regulatory networksS Balaji
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
J Mol Biol 360:204-12. 2006..Thus, an "over-engineered" backup system in the form of distributed architecture is likely to be a major determinant of the "evolvability" of the gene expression in organisms faced with environmental diversity...
Prediction of novel families of enzymes involved in oxidative and other complex modifications of bases in nucleic acidsLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Cell Cycle 8:1698-710. 2009....
Evolution of the deaminase fold and multiple origins of eukaryotic editing and mutagenic nucleic acid deaminases from bacterial toxin systemsLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Nucleic Acids Res 39:9473-97. 2011..These observations greatly expand the distribution of possible unidentified mutagenic processes catalyzed by nucleic acid deaminases...
Unraveling the biochemistry and provenance of pupylation: a prokaryotic analog of ubiquitinationLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Biol Direct 3:45. 2008..Reviewers: This article was reviewed by M. Madan Babu and Andrei Osterman...
Estimating the prevalence and regulatory potential of the telomere looping effect in yeast transcription regulationM Madan Babu
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
Cell Cycle 5:2354-63. 2006..Based on these observations, we also propose that genome-wide chromatin immunoprecipitation data might be useful to systematically uncover long-range chromatin looping effects in gene expression...
Anatomy of the E2 ligase fold: implications for enzymology and evolution of ubiquitin/Ub-like protein conjugationA Maxwell Burroughs
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
J Struct Biol 162:205-18. 2008..These features are likely to form a critical mechanical element of the fold required for catalysis. The results presented here could aid in new experiments to understand E2 catalysis...
The HIRAN domain and recruitment of chromatin remodeling and repair activities to damaged DNALakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
Cell Cycle 5:775-82. 2006....
Amidoligases with ATP-grasp, glutamine synthetase-like and acetyltransferase-like domains: synthesis of novel metabolites and peptide modifications of proteinsLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Mol Biosyst 5:1636-60. 2009..We present an evolutionary scenario for the multiple convergent origins of peptide ligases in various folds and clarify the bacterial origin of eukaryotic peptide-tagging enzymes of the TTL family...
Comparative genomics, evolution and origins of the nuclear envelope and nuclear pore complexBen J Mans
National Center for Biotechnology Information, National Institutes of Health, Bethesda, Maryland 20892, USA
Cell Cycle 3:1612-37. 2004..A primitive NPC with approximately 20 proteins and a Src1p-Man1-like membrane protein with a DNA-tethering HEH domain are inferred to have been integral perinuclear components in the las common ancestor of modern eukaryotes...
Discovering functional linkages and uncharacterized cellular pathways using phylogenetic profile comparisons: a comprehensive assessmentRaja Jothi
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
BMC Bioinformatics 8:173. 2007..Here, proteins with similar profiles are inferred to be functionally related under the assumption that proteins involved in the same metabolic pathway or cellular system are likely to have been co-inherited during evolution...
HPC2 and ubinuclein define a novel family of histone chaperones conserved throughout eukaryotesS Balaji
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Mol Biosyst 5:269-75. 2009..Hence, we propose that Hpc2p homologs are probably involved in recruitment of the ancient conserved histone-loading Hir-Asf1 complex to different lineage-specific chromatin reorganization processes...
Natural history of the E1-like superfamily: implication for adenylation, sulfur transfer, and ubiquitin conjugationA Maxwell Burroughs
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
Proteins 75:895-910. 2009..In light of this, we present evidence that in eukaryotes other E1-like enzymes such as Urm1 were independently recruited for Ubl conjugation, probably functioning without conventional E2-like enzymes...
Discovery of the principal specific transcription factors of Apicomplexa and their implication for the evolution of the AP2-integrase DNA binding domainsS Balaji
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Nucleic Acids Res 33:3994-4006. 2005....
The SHS2 module is a common structural theme in functionally diverse protein groups, like Rpb7p, FtsA, GyrI, and MTH1598/TM1083 superfamiliesV Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
Proteins 56:795-807. 2004..Thus, the SHS2 domain is an example of a simple module that has been adapted to perform an entire spectrum of functions ranging from protein-protein interactions to small-molecule recognition and catalysis...
Monophyly of class I aminoacyl tRNA synthetase, USPA, ETFP, photolyase, and PP-ATPase nucleotide-binding domains: implications for protein evolution in the RNAL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland, USA
Proteins 48:1-14. 2002..Thus, substantial evolutionary diversification of protein domains occurred well before the modern version of the protein-dependent translation machinery was established, i.e., still in the RNA world...
Fold prediction and evolutionary analysis of the POZ domain: structural and evolutionary relationship with the potassium channel tetramerization domainL Aravind
National Center for Biotechnology Information, National Library of Medicine, Bethesda, MD, 20894, USA
J Mol Biol 285:1353-61. 1999..Phylogenetic analysis of the POZ domains suggests that the common ancestor of the crown group eukaryotes already possessed this domain; POZ domains have undergone independent expansion in plants and in different animal lineages...
Differential action of natural selection on the N and C-terminal domains of 2'-5' oligoadenylate synthetases and the potential nuclease function of the C-terminal domainIgor B Rogozin
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
J Mol Biol 326:1449-61. 2003....
Evolutionary genomics of the HAD superfamily: understanding the structural adaptations and catalytic diversity in a superfamily of phosphoesterases and allied enzymesA Maxwell Burroughs
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
J Mol Biol 361:1003-34. 2006..The newly identified relationships and functional predictions provided here are likely to aid the future exploration of the numerous poorly understood members of this large superfamily of enzymes...
The WWE domain: a common interaction module in protein ubiquitination and ADP ribosylationL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Trends Biochem Sci 26:273-5. 2001..It was named the WWE domain after its most conserved residues and is predicted to mediate specific protein-protein interactions in ubiquitin and ADP-ribose conjugation systems...
The PRC-barrel: a widespread, conserved domain shared by photosynthetic reaction center subunits and proteins of RNA metabolismVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Genome Biol 3:RESEARCH0061. 2002..It contains a distinct cytoplasmic beta-barrel domain whose fold has no close structural relationship to any other well known beta-barrel domain...
A novel superfamily of predicted cysteine proteases from eukaryotes, viruses and Chlamydia pneumoniaeK S Makarova
Dept of Pathology, F.E. Hebert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799, USA
Trends Biochem Sci 25:50-2. 2000
Differences in evolutionary pressure acting within highly conserved ortholog groupsTeresa M Przytycka
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
BMC Evol Biol 8:208. 2008..g. Proteobacteria or Firmicutes)...
The bacterial replicative helicase DnaB evolved from a RecA duplicationD D Leipe
National Center for Biotechnology Information NCBI, National Library of Medicine, National Institutes of Health, Bethesda Maryland 20894 USA
Genome Res 10:5-16. 2000....
The CHASE domain: a predicted ligand-binding module in plant cytokinin receptors and other eukaryotic and bacterial receptorsV Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Trends Biochem Sci 26:579-82. 2001..The domain is predicted to bind diverse low molecular weight ligands, such as the cytokinin-like adenine derivatives or peptides, and mediate signal transduction through the respective receptors...
Novel predicted peptidases with a potential role in the ubiquitin signaling pathwayLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
Cell Cycle 3:1440-50. 2004..More specifically, genetic evidence implicates the WLM family in de-SUMOylation. If validated experimentally, the WLM family proteins will represent the first case of a Zincin-like metalloprotease involvement in Ub-signaling...
The GOLD domain, a novel protein module involved in Golgi function and secretionVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Genome Biol 3:research0023. 2002....
Evolutionary dynamics of prokaryotic transcriptional regulatory networksM Madan Babu
National Center for Biotechnology Information, National Institutes of Health, MD 20894, USA
J Mol Biol 358:614-33. 2006..The methods for biological network analysis introduced here can be applied generally to study other networks, and these predictions can be used to guide specific experiments...
BEN: a novel domain in chromatin factors and DNA viral proteinsSaraswathi Abhiman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Bioinformatics 24:458-61. 2008..The presence of BEN domains in a poxviral early virosomal protein and in polydnaviral proteins also suggests a possible role for them in organization of viral DNA during replication or transcription...
Molecular cloning, expression, and structural prediction of deoxyhypusine hydroxylase: a HEAT-repeat-containing metalloenzymeJong Hwan Park
Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research and National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 103:51-6. 2006..However, metal coordination sites composed of four strictly conserved histidine-glutamate sequences were identified, suggesting that DOHH enzymes have convergently evolved an iron-dependent hydroxylation mechanism...
Comparative genomics of transcription factors and chromatin proteins in parasitic protists and other eukaryotesLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Int J Parasitol 38:1-31. 2008..This evolutionary synthesis offers a robust scaffold for future investigation of transcription and chromatin structure in parasitic protists...
MOSC domains: ancient, predicted sulfur-carrier domains, present in diverse metal-sulfur cluster biosynthesis proteins including Molybdenum cofactor sulfurasesVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
FEMS Microbiol Lett 207:55-61. 2002..The identification of this domain may clarify the mechanism of biogenesis of various metallo-enzymes including Molybdenum cofactor-containing enzymes that are compromised in human type II xanthinuria...
Classification and evolution of P-loop GTPases and related ATPasesDetlef D Leipe
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
J Mol Biol 317:41-72. 2002..In addition to the classification of previously known families of GTPases and related ATPases, we introduce several previously undetected families and describe new functional predictions...
The NYN domains: novel predicted RNAses with a PIN domain-like foldVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
RNA Biol 3:18-27. 2006..Given the localization of Nedd4-BP1 to punctuate nuclear bodies, it is likely that they are parts of nuclear RNA-processing complexes that are dependent on monoubiquitination for their assembly...
Evolution of aminoacyl-tRNA synthetases--analysis of unique domain architectures and phylogenetic trees reveals a complex history of horizontal gene transfer eventsY I Wolf
National Center for Biotechnology Information NCBI, National Library of Medicine, National Institutes of Health NIH, Bethesda Maryland 20894, USA
Genome Res 9:689-710. 1999....
SPOUT: a class of methyltransferases that includes spoU and trmD RNA methylase superfamilies, and novel superfamilies of predicted prokaryotic RNA methylasesVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
J Mol Microbiol Biotechnol 4:71-5. 2002
Trends in protein evolution inferred from sequence and structure analysisL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Curr Opin Struct Biol 12:392-9. 2002....
OST-HTH: a novel predicted RNA-binding domainVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Biol Direct 5:13. 2010..Using sequence profile searches we identify a novel domain in these proteins that is widely conserved across eukaryotes and bacteria...
Comparative genomics and evolution of proteins involved in RNA metabolismVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, 8600 Rockville Pike, Building 389, National Institutes of Health, Bethesda, MD 20894, USA
Nucleic Acids Res 30:1427-64. 2002..In addition to the reconstruction of the evolutionary history of RNA metabolism, this analysis produced numerous functional predictions, e.g. of previously undetected enzymes of RNA modification...
STAND, a class of P-loop NTPases including animal and plant regulators of programmed cell death: multiple, complex domain architectures, unusual phyletic patterns, and evolution by horizontal gene transferDetlef D Leipe
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
J Mol Biol 343:1-28. 2004..Transfer of genes for STAND NTPases from bacteria to eukaryotes on several occasions might have played a significant role in the evolution of eukaryotic signaling systems...
New connections in the prokaryotic toxin-antitoxin network: relationship with the eukaryotic nonsense-mediated RNA decay systemVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Genome Biol 4:R81. 2003..The RelE toxin cleaves ribosome-associated transcripts, whereas another post-segregational cell killing toxin, ParE, functions as a gyrase inhibitor...
Evolution and classification of P-loop kinases and related proteinsDetlef D Leipe
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
J Mol Biol 333:781-815. 2003..The results could serve as a framework for systematic experimental characterization of new biochemical and biological functions of kinases...
DOMON: an ancient extracellular domain in dopamine beta-monooxygenase and other proteinsL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda MD 20894, USA
Trends Biochem Sci 26:524-6. 2001..This domain was predicted to adopt an all-beta fold with seven or eight strands and might function as a module mediating a range of extracellular adhesive interactions...
Evolutionary history, structural features and biochemical diversity of the NlpC/P60 superfamily of enzymesVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Genome Biol 4:R11. 2003..Currently characterized members are known to hydrolyze D-gamma-glutamyl-meso-diaminopimelate or N-acetylmuramate-L-alanine linkages...
Emergence of diverse biochemical activities in evolutionarily conserved structural scaffolds of proteinsVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Curr Opin Chem Biol 7:12-20. 2003..The biochemical plasticity of a fold seems to hinge on the presence of a generic, symmetrical substrate-binding pocket as opposed to highly specialized binding sites...
A database of bacterial lipoproteins (DOLOP) with functional assignments to predicted lipoproteinsM Madan Babu
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
J Bacteriol 188:2761-73. 2006..The website, along with additional information on the biosynthetic pathway, statistics on predicted lipoproteins, and related figures, is available at http://www.mrc-lmb.cam.ac.uk/genomes/dolop/...
An antisense RNA controls synthesis of an SOS-induced toxin evolved from an antitoxinMitsuoki Kawano
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Mol Microbiol 64:738-54. 2007..We suggest that SymE promotion of RNA cleavage may be important for the recycling of RNAs damaged under SOS-inducing conditions...
Peptide-N-glycanases and DNA repair proteins, Xp-C/Rad4, are, respectively, active and inactivated enzymes sharing a common transglutaminase foldV Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Hum Mol Genet 10:1627-30. 2001..The RAD4/Xp-C proteins, along with other inactive transglutaminase-fold proteins, represent a case of functional re-assignment of an ancient domain following the loss of the ancestral enzymatic activity...
A bipolar DNA helicase gene, herA, clusters with rad50, mre11 and nurA genes in thermophilic archaeaF Constantinesco
, UMR CNRS no8621, , , 91405 Orsay Cedex, France
Nucleic Acids Res 32:1439-47. 2004....
Role of Rpn11 metalloprotease in deubiquitination and degradation by the 26S proteasomeRati Verma
Department of Biology and Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA
Science 298:611-5. 2002..Our findings reveal an unexpected coupling between substrate deubiquitination and degradation and suggest a unifying rationale for the presence of the lid in eukaryotic proteasomes...
Complete genome sequence of the apicomplexan, Cryptosporidium parvumMitchell S Abrahamsen
Department of Veterinary and Biomedical Science, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108, USA
Science 304:441-5. 2004..Elucidation of the core metabolism, including enzymes with high similarities to bacterial and plant counterparts, opens new avenues for drug development...
Human and mouse homologs of Escherichia coli DinB (DNA polymerase IV), members of the UmuC/DinB superfamilyV L Gerlach
Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75235 9072, USA
Proc Natl Acad Sci U S A 96:11922-7. 1999..In a phylogenetic tree, the mouse and human DinB1 proteins specifically group with E. coli DinB, suggesting a mitochondrial origin for these genes. The human DINB1 gene is localized to chromosome 5q13 and is widely expressed...
Comparative genomic analysis of archaeal genotypic variants in a single population and in two different oceanic provincesOded Beja
Monterey Bay Aquarium Research Institute, Moss Landing, California 95039, USA
Appl Environ Microbiol 68:335-45. 2002..Our results also demonstrate that genomic approaches can provide high-resolution information relevant to microbial population genetics, ecology, and evolution, even for microbes that have not yet been cultivated...
Identification of the prokaryotic ligand-gated ion channels and their implications for the mechanisms and origins of animal Cys-loop ion channelsAsba Tasneem
Beckman Institute, University of Illinois at Urbana-Champaign, 405 N Mathews Avenue, Urbana, IL 61801, USA
Genome Biol 6:R4. 2005..The phyletic patterns and phylogenetic relationships suggest the possibility that the metazoan receptors emerged through an early lateral transfer from a prokaryotic source, before the divergence of extant metazoan lineages...
YjeQ, an essential, conserved, uncharacterized protein from Escherichia coli, is an unusual GTPase with circularly permuted G-motifs and marked burst kineticsDenis M Daigle
Antimicrobial Research Centre, Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada L8N 3Z5
Biochemistry 41:11109-17. 2002..8 h(-)(1)). In summary, E. coli YjeQ is an unusual, circularly permuted P-loop-containing GTPase, which catalyzes GTP hydrolysis at a rate 45 000 times greater than that of turnover...
Cloning the human and mouse MMS19 genes and functional complementation of a yeast mms19 deletion mutantL Queimado
Laboratory of Molecular Pathology, Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9072, USA
Nucleic Acids Res 29:1884-91. 2001..Both the mouse and human genes are ubiquitously expressed as multiple transcripts, some of which appear to derive from alternative splicing. The ratio of different transcripts varies in several different tissue types...
