Research Topics
Genomes and GenesSpecies
| L AravindSummaryAffiliation: National Institutes of Health Country: USA Publications
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Detail Information
Publications
Gene flow and biological conflict systems in the origin and evolution of eukaryotesL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda MD, USA
Front Cell Infect Microbiol 2:89. 2012....
ALOG domains: provenance of plant homeotic and developmental regulators from the DNA-binding domain of a novel class of DIRS1-type retroposonsLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, 20894, USA
Biol Direct 7:39. 2012..Hence, they add to the growing evidence for derivation of DNA-binding domains of eukaryotic specific TFs from mobile and selfish elements...
Ter-dependent stress response systems: novel pathways related to metal sensing, production of a nucleoside-like metabolite, and DNA-processingVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
Mol Biosyst 8:3142-65. 2012..In some eukaryotes, certain components of the ter system appear to be recruited to function in conjunction with the ubiquitin system and calcium-signaling pathways...
Female Anopheles gambiae antennae: increased transcript accumulation of the mosquito-specific odorant-binding-protein OBP2Seth A Hoffman
Sanaria Inc, 9800 Medical Center Dr, Rockville, MD 20850, USA
Parasit Vectors 5:27. 2012..Understanding olfactory function could lead to development of control strategies based on repelling parasite-carrying mosquitoes or attracting them into a fatal trap...
Identification of novel components of NAD-utilizing metabolic pathways and prediction of their biochemical functionsRobson Francisco de Souza
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Mol Biosyst 8:1661-77. 2012..We use the data derived from this analysis to understand the origin and early evolutionary trajectories of key NAD-utilizing enzymes and present targets for future biochemical investigations...
The HARE-HTH and associated domains: novel modules in the coordination of epigenetic DNA and protein modificationsL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA
Cell Cycle 11:119-31. 2012....
Reconstructing prokaryotic transcriptional regulatory networks: lessons from actinobacteriaThiago M Venancio
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
J Biol 8:29. 2009..Recent studies, including one in BMC Systems Biology, address this problem in the relatively underexplored actinobacteria clade, which includes major pathogenic and economically relevant taxa...
Evolutionary history, structural features and biochemical diversity of the NlpC/P60 superfamily of enzymesVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Genome Biol 4:R11. 2003..Currently characterized members are known to hydrolyze D-gamma-glutamyl-meso-diaminopimelate or N-acetylmuramate-L-alanine linkages...
Reconstructing the ubiquitin network: cross-talk with other systems and identification of novel functionsThiago M Venancio
National Center for Biotechnology Information, National Library of Medicine, NIH, Bethesda, MD 20894, USA
Genome Biol 10:R33. 2009..While several studies have concentrated on structure-function relationships and evolution of individual components of the Ub-system, a study of the system as a whole is largely lacking...
New connections in the prokaryotic toxin-antitoxin network: relationship with the eukaryotic nonsense-mediated RNA decay systemVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Genome Biol 4:R81. 2003..The RelE toxin cleaves ribosome-associated transcripts, whereas another post-segregational cell killing toxin, ParE, functions as a gyrase inhibitor...
The PRC-barrel: a widespread, conserved domain shared by photosynthetic reaction center subunits and proteins of RNA metabolismVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Genome Biol 3:RESEARCH0061. 2002..It contains a distinct cytoplasmic beta-barrel domain whose fold has no close structural relationship to any other well known beta-barrel domain...
The GOLD domain, a novel protein module involved in Golgi function and secretionVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Genome Biol 3:research0023. 2002....
Identification of the prokaryotic ligand-gated ion channels and their implications for the mechanisms and origins of animal Cys-loop ion channelsAsba Tasneem
Beckman Institute, University of Illinois at Urbana-Champaign, 405 N Mathews Avenue, Urbana, IL 61801, USA
Genome Biol 6:R4. 2005..The phyletic patterns and phylogenetic relationships suggest the possibility that the metazoan receptors emerged through an early lateral transfer from a prokaryotic source, before the divergence of extant metazoan lineages...
Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR-Cas systemsKira S Makarova
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, 8600 Rockville Pike, Bethesda, MD 20894, USA
Biol Direct 6:38. 2011..Comparative analysis of the Cas protein sequences and structures led to the classification of the CRISPR-Cas systems into three Types (I, II and III)...
A new family of polymerases related to superfamily A DNA polymerases and T7-like DNA-dependent RNA polymerasesLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Biol Direct 3:39. 2008..They also provide evidence that enigmatic DNA viruses, such as Sputnik, might have emerged from mobile elements coding these polymerases...
OST-HTH: a novel predicted RNA-binding domainVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Biol Direct 5:13. 2010..Using sequence profile searches we identify a novel domain in these proteins that is widely conserved across eukaryotes and bacteria...
The Anabaena sensory rhodopsin transducer defines a novel superfamily of prokaryotic small-molecule binding domainsRobson F de Souza
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Biol Direct 4:25; discussion 25. 2009..The analysis of this superfamily also provides new data to investigate the evolution of carbohydrate binding modes in beta-sandwich domains with very different topologies...
Presence of a classical RRM-fold palm domain in Thg1-type 3'- 5'nucleic acid polymerases and the origin of the GGDEF and CRISPR polymerase domainsVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Biol Direct 5:43. 2010....
Novel eukaryotic enzymes modifying cell-surface biopolymersVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Biol Direct 5:1. 2010..Using comparative genomics and sequence profile analysis we identify several novel enzymes that could be potentially involved in the modification of cell-surface glycans or glycoproteins...
Evolutionary connection between the catalytic subunits of DNA-dependent RNA polymerases and eukaryotic RNA-dependent RNA polymerases and the origin of RNA polymerasesLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
BMC Struct Biol 3:1. 2003..No evolutionary relationship between RDRP and other polymerases has been reported so far, hence the origin of this eukaryote-specific polymerase remains a mystery...
Ancient conserved domains shared by animal soluble guanylyl cyclases and bacterial signaling proteinsLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
BMC Genomics 4:5. 2003..Soluble guanylyl cyclases (SGCs) are dimeric enzymes that transduce signals downstream of nitric oxide (NO) in animals. They sense NO by means of a heme moiety that is bound to their N-terminal extensions...
Differences in evolutionary pressure acting within highly conserved ortholog groupsTeresa M Przytycka
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
BMC Evol Biol 8:208. 2008..g. Proteobacteria or Firmicutes)...
Discovering functional linkages and uncharacterized cellular pathways using phylogenetic profile comparisons: a comprehensive assessmentRaja Jothi
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
BMC Bioinformatics 8:173. 2007..Here, proteins with similar profiles are inferred to be functionally related under the assumption that proteins involved in the same metabolic pathway or cellular system are likely to have been co-inherited during evolution...
Extensive domain shuffling in transcription regulators of DNA viruses and implications for the origin of fungal APSES transcription factorsLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Genome Biol 3:RESEARCH0012. 2002....
Monophyly of class I aminoacyl tRNA synthetase, USPA, ETFP, photolyase, and PP-ATPase nucleotide-binding domains: implications for protein evolution in the RNAL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland, USA
Proteins 48:1-14. 2002..Thus, substantial evolutionary diversification of protein domains occurred well before the modern version of the protein-dependent translation machinery was established, i.e., still in the RNA world...
Intraproteomic networks: new forays into predicting interaction partnersL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland, 20894, USA
Genome Res 12:1156-8. 2002
Plasmodium biology: genomic gleaningsL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Cell 115:771-85. 2003..Plasmodium possesses many cell surface molecules with "animal-like" adhesion modules. Potential genetic footprints of the ancestral eukaryotic algal precursor of the apicoplast are also detectable in its genome...
The SWIRM domain: a conserved module found in chromosomal proteins points to novel chromatin-modifying activitiesL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Genome Biol 3:RESEARCH0039. 2002..These modifications have an important role in the regulation of chromatin structure and are mediated by large multisubunit complexes that contain modular proteins with several conserved catalytic and noncatalytic adaptor domains...
Evolutionary connections between bacterial and eukaryotic signaling systems: a genomic perspectiveL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Curr Opin Microbiol 6:490-7. 2003....
A novel family of P-loop NTPases with an unusual phyletic distribution and transmembrane segments inserted within the NTPase domainL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Genome Biol 5:R30. 2004..These studies provide a framework for characterization of novel lineages within this fold and prediction of their functional properties...
The two faces of Alba: the evolutionary connection between proteins participating in chromatin structure and RNA metabolismL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Genome Biol 4:R64. 2003..Here we use comparative genomics and sequence profile analysis to predict potential alternative functions of the Alba proteins...
HutC/FarR-like bacterial transcription factors of the GntR family contain a small molecule-binding domain of the chorismate lyase foldL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
FEMS Microbiol Lett 222:17-23. 2003..An ancestral, generic small-molecule-binding domain appears to have given rise to the enzymatic and non-catalytic ligand-binding versions of the same fold under the influence of different selective pressures...
Provenance of SET-domain histone methyltransferases through duplication of a simple structural unitL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
Cell Cycle 2:369-76. 2003..Thus, the SET domain provides a model for the innovation of a complex enzymatic fold through the duplications of a structurally simple non-enzymatic unit...
The many faces of the helix-turn-helix domain: transcription regulation and beyondL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Building 38A, Room 5N50, Bethesda, MD 20894, USA
FEMS Microbiol Rev 29:231-62. 2005..This differential relationship of their basal and specific transcriptional machinery poses an apparent conundrum regarding the origins of their transcription apparatus...
MEDS and PocR are novel domains with a predicted role in sensing simple hydrocarbon derivatives in prokaryotic signal transduction systemsVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Bioinformatics 21:2805-11. 2005..Hence it is possible that these sigma factors, involved in aerial mycelium development and stress response in the actinomycetes, might be under the regulation of as yet uncharacterized small molecules. CONTACT: ...
Fold prediction and evolutionary analysis of the POZ domain: structural and evolutionary relationship with the potassium channel tetramerization domainL Aravind
National Center for Biotechnology Information, National Library of Medicine, Bethesda, MD, 20894, USA
J Mol Biol 285:1353-61. 1999..Phylogenetic analysis of the POZ domains suggests that the common ancestor of the crown group eukaryotes already possessed this domain; POZ domains have undergone independent expansion in plants and in different animal lineages...
Classification of the caspase-hemoglobinase fold: detection of new families and implications for the origin of the eukaryotic separinsL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland, USA
Proteins 46:355-67. 2002..The acquisition of the caspase-hemoglobinase-fold domains by eukaryotes might have been critical in the evolution of important eukaryotic processes, such as mitosis and programmed cell death...
The DNA-repair protein AlkB, EGL-9, and leprecan define new families of 2-oxoglutarate- and iron-dependent dioxygenasesL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Genome Biol 2:RESEARCH0007. 2001....
Apprehending multicellularity: regulatory networks, genomics, and evolutionL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
Birth Defects Res C Embryo Today 87:143-64. 2009....
SURVEY AND SUMMARY: holliday junction resolvases and related nucleases: identification of new families, phyletic distribution and evolutionary trajectoriesL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Nucleic Acids Res 28:3417-32. 2000..This analysis resulted in the prediction of numerous previously unnoticed nucleases, some of which are likely to be new restriction enzymes...
Eukaryote-specific domains in translation initiation factors: implications for translation regulation and evolution of the translation systemL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institute of Health, Bethesda, MD 20894, USA
Genome Res 10:1172-84. 2000....
Predicted class-I aminoacyl tRNA synthetase-like proteins in non-ribosomal peptide synthesisL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Biol Direct 5:48. 2010..The least studied of these systems are those utilizing tRNAs or aminoacyl-tRNA synthetases (AAtRS) in non-ribosomal peptide ligation...
Origin of multicellular eukaryotes - insights from proteome comparisonsL Aravind
Department of Biology, Texas A and M University, College Station, 77843, USA
Curr Opin Genet Dev 9:688-94. 1999..Similar comparisons with the available sequence data from the plant Arabidopsis thaliana produce consistent results. These observations have provided useful insights regarding the origin of multicellular organisms...
The alpha/beta fold uracil DNA glycosylases: a common origin with diverse fatesL Aravind
National Center for Biotechnology Information, National Institutes of Health, Bethesda, MD 20894, USA
Genome Biol 1:RESEARCH0007. 2000..The structural and evolutionary relationships between the rest of the UDGs remain uncertain...
DNA polymerase beta-like nucleotidyltransferase superfamily: identification of three new families, classification and evolutionary historyL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Nucleic Acids Res 27:1609-18. 1999....
THUMP--a predicted RNA-binding domain shared by 4-thiouridine, pseudouridine synthases and RNA methylasesL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Trends Biochem Sci 26:215-7. 2001..We predict that this domain is an RNA-binding domain that adopts an alpha/beta fold similar to that found in the C-terminal domain of translation initiation factor 3 and ribosomal protein S8...
The WWE domain: a common interaction module in protein ubiquitination and ADP ribosylationL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Trends Biochem Sci 26:273-5. 2001..It was named the WWE domain after its most conserved residues and is predicted to mediate specific protein-protein interactions in ubiquitin and ADP-ribose conjugation systems...
Domain architectures of the Scm3p protein provide insights into centromere function and evolutionL Aravind
National Center for Biotechnology Information, National Library of Medicine, Bethesda, Maryland, USA
Cell Cycle 6:2511-5. 2007..Hence, the C-terminal DNA binding motifs found in large Scm3p proteins may, along with CenH3, serve as a key epigenetic signal by recognizing and accommodating the lineage-specific diversity of centromere DNA in course of evolution...
Prokaryotic homologs of the eukaryotic DNA-end-binding protein Ku, novel domains in the Ku protein and prediction of a prokaryotic double-strand break repair systemL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
Genome Res 11:1365-74. 2001..A hypothesis is proposed that the Ku homolog, together with the associated gene products, comprise a previously unrecognized prokaryotic system for repair of double-strand breaks in DNA...
Novel conserved domains in proteins with predicted roles in eukaryotic cell-cycle regulation, decapping and RNA stabilityVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
BMC Genomics 5:45. 2004..The identification of these RNA-binding domains may throw light on the emergence of eukaryote-specific systems of RNA metabolism...
Origin and evolution of the archaeo-eukaryotic primase superfamily and related palm-domain proteins: structural insights and new membersLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health Bethesda, MD 20894, USA
Nucleic Acids Res 33:3875-96. 2005..These observations point to a functional equivalence of the two classes of primases, which seem to have repeatedly displaced each other in various extrachromosomal replicons...
Regulatory potential, phyletic distribution and evolution of ancient, intracellular small-molecule-binding domainsV Anantharaman
National Center for Biotechnology Information National Library of Medicine, National Institutes of Health, Bethesda, MD, 20894, USA
J Mol Biol 307:1271-92. 2001..Using the "guilt by association approach", the identification of SMBDs allowed prediction of functions and mode of regulation for a variety of previously uncharacterized proteins...
SWIM, a novel Zn-chelating domain present in bacteria, archaea and eukaryotesKira S Makarova
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Trends Biochem Sci 27:384-6. 2002..This domain was designated SWIM after SWI2/SNF2 and MuDR, and is predicted to have DNA-binding and protein-protein interaction functions in different contexts...
The natural history of the WRKY-GCM1 zinc fingers and the relationship between transcription factors and transposonsM Madan Babu
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Nucleic Acids Res 34:6505-20. 2006..We also use the WRKY-GCM1 superfamily as an example to illustrate the importance of transposons in the emergence of new TFs in different lineages...
Comparative genomics of the FtsK-HerA superfamily of pumping ATPases: implications for the origins of chromosome segregation, cell division and viral capsid packagingLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Nucleic Acids Res 32:5260-79. 2004....
Detection of novel members, structure-function analysis and evolutionary classification of the 2H phosphoesterase superfamilyRaja Mazumder
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Nucleic Acids Res 30:5229-43. 2002..Initially, this domain appears to have been involved in RNA processing and it appears to have been recruited to perform various other functions in later stages of evolution...
Comprehensive analysis of combinatorial regulation using the transcriptional regulatory network of yeastS Balaji
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda 20894, USA
J Mol Biol 360:213-27. 2006..We believe that the results presented here might provide a framework for testing the role of co-regulatory associations in eukaryotic transcriptional control...
Quoderat demonstrandum? The mystery of experimental validation of apparently erroneous computational analyses of protein sequencesL M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Genome Biol 2:RESEARCH0051. 2001..Computational predictions are critical for directing the experimental study of protein functions. Therefore it is paradoxical when an apparently erroneous computational prediction seems to be supported by experiment...
A DNA repair system specific for thermophilic Archaea and bacteria predicted by genomic context analysisKira S Makarova
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Building 380, Bethesda, MD 20894, USA
Nucleic Acids Res 30:482-96. 2002....
Origin and evolution of peptide-modifying dioxygenases and identification of the wybutosine hydroxylase/hydroperoxidaseLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Nucleic Acids Res 38:5261-79. 2010..The specificity of wybutosine hydroxylase/peroxidase probably relates to the structural similarity of the modified moiety to the ancestral amino acid substrate of this superfamily...
Prediction of the archaeal exosome and its connections with the proteasome and the translation and transcription machineries by a comparative-genomic approachE V Koonin
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
Genome Res 11:240-52. 2001....
Eukaryotic signalling domain homologues in archaea and bacteria. Ancient ancestry and horizontal gene transferC P Ponting
National Center for Biotechnology Information National Library of Medicine, National Institutes of Health, Bldg 38A, Bethesda, MD, 20894, USA
J Mol Biol 289:729-45. 1999..A number of transfers of signalling domains from eukaryotes to bacteria were confidently identified, in contrast to only a single case of apparent transfer from eukaryotes to archaea...
A novel superfamily of predicted cysteine proteases from eukaryotes, viruses and Chlamydia pneumoniaeK S Makarova
Dept of Pathology, F.E. Hebert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799, USA
Trends Biochem Sci 25:50-2. 2000
Polymorphic toxin systems: Comprehensive characterization of trafficking modes, processing, mechanisms of action, immunity and ecology using comparative genomicsDapeng Zhang
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Biol Direct 7:18. 2012..Prior analysis revealed a striking diversity of nuclease and deaminase domains among the toxin modules. We systematically investigated polymorphic toxin systems using comparative genomics, sequence and structure analysis...
Genome of the extremely radiation-resistant bacterium Deinococcus radiodurans viewed from the perspective of comparative genomicsK S Makarova
Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799, USA
Microbiol Mol Biol Rev 65:44-79. 2001..In combination, these observations suggest that several different biological mechanisms contribute to the multiple DNA repair-dependent phenotypes of this organism...
TRAM, a predicted RNA-binding domain, common to tRNA uracil methylation and adenine thiolation enzymesV Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
FEMS Microbiol Lett 197:215-21. 2001..In addition, sequence analysis of the MiaB family enzymes showed that they share the predicted catalytic site with biotin and lipoate synthases and probably employ the same mechanism for sulfur insertion into their respective substrate...
Diversification of catalytic activities and ligand interactions in the protein fold shared by the sugar isomerases, eIF2B, DeoR transcription factors, acyl-CoA transferases and methenyltetrahydrofolate synthetaseVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
J Mol Biol 356:823-42. 2006....
IMPALA: matching a protein sequence against a collection of PSI-BLAST-constructed position-specific score matricesA A Schaffer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Bioinformatics 15:1000-11. 1999..Also, it is considerably faster when run with a large database of PSSMs than is BLAST or PSI-BLAST when run against the complete non-redundant protein database...
The role of lineage-specific gene family expansion in the evolution of eukaryotesOlivier Lespinet
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
Genome Res 12:1048-59. 2002..LSEs seem to be one of the principal means of adaptation and one of the most important sources of organizational and regulatory diversity in crown-group eukaryotes...
Evolution of bacterial RNA polymerase: implications for large-scale bacterial phylogeny, domain accretion, and horizontal gene transferLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Gene 335:73-88. 2004....
The emergence of catalytic and structural diversity within the beta-clip foldLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
Proteins 55:977-91. 2004..We also describe how assembly of this fold from a basic three-stranded unit has been utilized to accommodate a variety of activities in its different versions...
A novel immunity system for bacterial nucleic acid degrading toxins and its recruitment in various eukaryotic and DNA viral systemsDapeng Zhang
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Nucleic Acids Res 39:4532-52. 2011....
Role of predicted metalloprotease motif of Jab1/Csn5 in cleavage of Nedd8 from Cul1Gregory A Cope
Department of Biology, California Institute of Technology Caltech, Pasadena, CA 91125, USA
Science 298:608-11. 2002..We propose that JAMM isopeptidases play important roles in a variety of physiological pathways...
Application of comparative genomics in the identification and analysis of novel families of membrane-associated receptors in bacteriaVivek Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
BMC Genomics 4:34. 2003..So far, they are relatively rare in the prokaryotes, and are restricted to the well-characterized sensory rhodopsins of various phototropic prokaryotes...
The RAGNYA fold: a novel fold with multiple topological variants found in functionally diverse nucleic acid, nucleotide and peptide-binding proteinsS Balaji
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Nucleic Acids Res 35:5658-71. 2007..3) We also present evidence for a bacterial origin of the GYF domain and propose how this version of the fold might have been utilized in peptide interactions in the context of nucleoprotein complexes...
Unraveling the biochemistry and provenance of pupylation: a prokaryotic analog of ubiquitinationLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Biol Direct 3:45. 2008..Reviewers: This article was reviewed by M. Madan Babu and Andrei Osterman...
Adaptations of the helix-grip fold for ligand binding and catalysis in the START domain superfamilyL M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Proteins 43:134-44. 2001..Thus, the START domain superfamily is a rare case of the adaptation of a protein fold with a conserved ligand-binding mode for both a broad variety of catalytic activities and noncatalytic regulatory functions. Proteins 2001;43:134-144...
Prediction of novel families of enzymes involved in oxidative and other complex modifications of bases in nucleic acidsLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Cell Cycle 8:1698-710. 2009....
Apoptotic molecular machinery: vastly increased complexity in vertebrates revealed by genome comparisonsL Aravind
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Science 291:1279-84. 2001..A considerable repertoire of apoptotic protein domains was detected in Actinomycetes and Cyanobacteria, which suggests a major contribution of horizontal gene transfer to the early evolution of apoptosis...
Molecular correlates of experimental cerebral malaria detectable in whole bloodMiranda S Oakley
Division of Bacterial, Parasitic, and Allergenic Products Center for Biologics Evaluation and Research, Food and Drug Administration, Rockville, Maryland, USA
Infect Immun 79:1244-53. 2011..A subset of these mediators of ECM warrant validation in P. falciparum-infected young African children as diagnostic markers of CM...
Pathogenic roles of CD14, galectin-3, and OX40 during experimental cerebral malaria in miceMiranda S Oakley
Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics and Evaluation Research, Food and Drug Administration, Bethesda, MD, USA
PLoS ONE 4:e6793. 2009..We find that OX40, a marker of both T cell activation and memory, is selectively upregulated in the brain during ECM and its distribution among CD4(+) and CD8(+) T cells accumulated in the brain vasculature is approximately equal...
CYSTM, a novel cysteine-rich transmembrane module with a role in stress tolerance across eukaryotesThiago M Venancio
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Bioinformatics 26:149-52. 2010..Thus, CYSTM proteins are likely to be part of a novel cellular protective mechanism that is widely active in eukaryotes, including humans. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online...
Comparative genomics, evolution and origins of the nuclear envelope and nuclear pore complexBen J Mans
National Center for Biotechnology Information, National Institutes of Health, Bethesda, Maryland 20892, USA
Cell Cycle 3:1612-37. 2004..A primitive NPC with approximately 20 proteins and a Src1p-Man1-like membrane protein with a DNA-tethering HEH domain are inferred to have been integral perinuclear components in the las common ancestor of modern eukaryotes...
Gene duplication with displacement and rearrangement: origin of the bacterial replication protein PriB from the single-stranded DNA-binding protein SsbVladimir A Ponomarev
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA
J Mol Microbiol Biotechnol 5:225-9. 2003....
Uncovering a hidden distributed architecture behind scale-free transcriptional regulatory networksS Balaji
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
J Mol Biol 360:204-12. 2006..Thus, an "over-engineered" backup system in the form of distributed architecture is likely to be a major determinant of the "evolvability" of the gene expression in organisms faced with environmental diversity...
Evolutionary genomics of nucleo-cytoplasmic large DNA virusesLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Virus Res 117:156-84. 2006....
The SHS2 module is a common structural theme in functionally diverse protein groups, like Rpb7p, FtsA, GyrI, and MTH1598/TM1083 superfamiliesV Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
Proteins 56:795-807. 2004..Thus, the SHS2 domain is an example of a simple module that has been adapted to perform an entire spectrum of functions ranging from protein-protein interactions to small-molecule recognition and catalysis...
Evolutionary genomics of the HAD superfamily: understanding the structural adaptations and catalytic diversity in a superfamily of phosphoesterases and allied enzymesA Maxwell Burroughs
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
J Mol Biol 361:1003-34. 2006..The newly identified relationships and functional predictions provided here are likely to aid the future exploration of the numerous poorly understood members of this large superfamily of enzymes...
The CHASE domain: a predicted ligand-binding module in plant cytokinin receptors and other eukaryotic and bacterial receptorsV Anantharaman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Trends Biochem Sci 26:579-82. 2001..The domain is predicted to bind diverse low molecular weight ligands, such as the cytokinin-like adenine derivatives or peptides, and mediate signal transduction through the respective receptors...
The ASCH superfamily: novel domains with a fold related to the PUA domain and a potential role in RNA metabolismLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Bioinformatics 22:257-63. 2006....
Novel predicted peptidases with a potential role in the ubiquitin signaling pathwayLakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
Cell Cycle 3:1440-50. 2004..More specifically, genetic evidence implicates the WLM family in de-SUMOylation. If validated experimentally, the WLM family proteins will represent the first case of a Zincin-like metalloprotease involvement in Ub-signaling...
The bacterial replicative helicase DnaB evolved from a RecA duplicationD D Leipe
National Center for Biotechnology Information NCBI, National Library of Medicine, National Institutes of Health, Bethesda Maryland 20894 USA
Genome Res 10:5-16. 2000....
Evolutionary dynamics of prokaryotic transcriptional regulatory networksM Madan Babu
National Center for Biotechnology Information, National Institutes of Health, MD 20894, USA
J Mol Biol 358:614-33. 2006..The methods for biological network analysis introduced here can be applied generally to study other networks, and these predictions can be used to guide specific experiments...
Anatomy of the E2 ligase fold: implications for enzymology and evolution of ubiquitin/Ub-like protein conjugationA Maxwell Burroughs
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
J Struct Biol 162:205-18. 2008..These features are likely to form a critical mechanical element of the fold required for catalysis. The results presented here could aid in new experiments to understand E2 catalysis...
BEN: a novel domain in chromatin factors and DNA viral proteinsSaraswathi Abhiman
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Bioinformatics 24:458-61. 2008..The presence of BEN domains in a poxviral early virosomal protein and in polydnaviral proteins also suggests a possible role for them in organization of viral DNA during replication or transcription...
Differential action of natural selection on the N and C-terminal domains of 2'-5' oligoadenylate synthetases and the potential nuclease function of the C-terminal domainIgor B Rogozin
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
J Mol Biol 326:1449-61. 2003....
Phosphoesterase domains associated with DNA polymerases of diverse originsL Aravind
Department of Biology, Texas A and M University, College Station, TX 77843, USA
Nucleic Acids Res 26:3746-52. 1998..In these cases, the pyrophosphate may be hydrolyzed by a stand-alone phosphatase, and candidates for such a role were identified among bacterial PHP superfamily members...
Molecular cloning, expression, and structural prediction of deoxyhypusine hydroxylase: a HEAT-repeat-containing metalloenzymeJong Hwan Park
Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research and National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 103:51-6. 2006..However, metal coordination sites composed of four strictly conserved histidine-glutamate sequences were identified, suggesting that DOHH enzymes have convergently evolved an iron-dependent hydroxylation mechanism...
Discovery of the principal specific transcription factors of Apicomplexa and their implication for the evolution of the AP2-integrase DNA binding domainsS Balaji
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Nucleic Acids Res 33:3994-4006. 2005....
Estimating the prevalence and regulatory potential of the telomere looping effect in yeast transcription regulationM Madan Babu
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
Cell Cycle 5:2354-63. 2006..Based on these observations, we also propose that genome-wide chromatin immunoprecipitation data might be useful to systematically uncover long-range chromatin looping effects in gene expression...
The HIRAN domain and recruitment of chromatin remodeling and repair activities to damaged DNALakshminarayan M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
Cell Cycle 5:775-82. 2006....
