Research Topics
Genomes and Genes
| E AppellaSummaryAffiliation: National Institutes of Health Country: USA Publications
| Collaborators
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Detail Information
Publications
Post-translational modifications and activation of p53 by genotoxic stressesE Appella
Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Eur J Biochem 268:2764-72. 2001....- Murine 86- and 84-kDa heat shock proteins, cDNA sequences, chromosome assignments, and evolutionary originsS K Moore
Laboratory of Cell Biology, National Cancer Institute, Bethesda, Maryland 20892
J Biol Chem 264:5343-51. 1989..The findings presented herein suggest that HSP86 and HSP84 may have different functions... - H-2RIIBP expressed from a baculovirus vector binds to multiple hormone response elementsM S Marks
Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892
Mol Endocrinol 6:219-30. 1992..Taken together, these data demonstrate that H-2RIIBP is capable of binding to hormone response elements of a variety of genes. They suggest that H-2RIIBP may exert a pleiotropic function... 
H-2RIIBP, a member of the nuclear hormone receptor superfamily that binds to both the regulatory element of major histocompatibility class I genes and the estrogen response elementK Hamada
Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, Bethesda, MD 20892
Proc Natl Acad Sci U S A 86:8289-93. 1989..Sequences homologous to the H-2RIIBP gene are widely conserved in the animal kingdom. H-2RIIBP mRNA is expressed in many mouse tissues, in agreement with the distribution of the natural region II factor...- DNA damage activates p53 through a phosphorylation-acetylation cascadeK Sakaguchi
Laboratory of Cell Biology, National Cancer Institute NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
Genes Dev 12:2831-41. 1998..These results suggest that DNA damage enhances p53 activity as a transcription factor in part through carboxy-terminal acetylation that, in turn, is directed by amino-terminal phosphorylation... - The negative signaling molecule SH2 domain-containing inositol-polyphosphate 5-phosphatase (SHIP) binds to the tyrosine-phosphorylated beta subunit of the high affinity IgE receptorT Kimura
Laboratory of Immunology, NIDR, NCI, National Institutes of Health, Bethesda, Maryland 20892 1188, USA
J Biol Chem 272:13991-6. 1997.... - NMR structure of a specific DNA complex of Zn-containing DNA binding domain of GATA-1J G Omichinski
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892
Science 261:438-46. 1993..A large number of interactions are observed with the phosphate backbone... - Characterization of the mouse 84-kD heat shock protein gene familyS K Moore
Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892
DNA Cell Biol 9:387-400. 1990..The hsp84 gene family includes at least six different hsp84-related pseudogenes, which arose about 2-3 million years ago... - Identification of TRP-2 as a human tumor antigen recognized by cytotoxic T lymphocytesR F Wang
National Cancer Institute, Bethesda, Maryland 20892, USA
J Exp Med 184:2207-16. 1996..Like other melamona differentiation antigens, TRP-2 was only expressed in melanoma, melanocytes, and retina, but not in other human tissues tested... - Initiation of a G2/M checkpoint after ultraviolet radiation requires p38 kinaseD V Bulavin
Division of Basic Science, National Cancer Intitute, National Institutes of Health, Bethesda, MD 20892, USA
Nature 411:102-7. 2001..We propose that regulation of Cdc25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation... - Identification of a human melanoma antigen recognized by tumor-infiltrating lymphocytes associated with in vivo tumor rejectionY Kawakami
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892
Proc Natl Acad Sci U S A 91:6458-62. 1994.... - High-resolution three-dimensional structure of a single zinc finger from a human enhancer binding protein in solutionJ G Omichinski
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892
Biochemistry 29:9324-34. 1990.... - Syk-independent tyrosine phosphorylation and association of the protein tyrosine phosphatases SHP-1 and SHP-2 with the high affinity IgE receptorT Kimura
Laboratory of Immunology, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 159:4426-34. 1997..Thus, unlike other immune cells in which inhibitory molecules are recruited by accessory proteins, Fc epsilonRI bind molecules that both activate and inhibit signal transduction... - High-resolution structure of the oligomerization domain of p53 by multidimensional NMRG M Clore
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892
Science 265:386-91. 1994..The implications of the structure of the tetramer for the biological function of p53 are discussed... - A model for melanosome biogenesis based on the purification and analysis of early melanosomesT Kushimoto
Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 98:10698-703. 2001..These results provide a better understanding of the structural features seen during melanosome biogenesis, and they yield further clues as to the physiological regulation of pigmentation... - Phosphorylation of human p53 by p38 kinase coordinates N-terminal phosphorylation and apoptosis in response to UV radiationD V Bulavin
Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
EMBO J 18:6845-54. 1999..These results suggest that p38 kinase plays a prominent role in an integrated regulation of N-terminal phosphorylation that regulates p53-mediated apoptosis after UV radiation... - p53 regulates the expression of the tumor suppressor gene maspinZ Zou
Department of Surgery, Center for Prostate Disease Research, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
J Biol Chem 275:6051-4. 2000.... - The ezrin-like family of tyrosine kinase substrates: receptor-specific pattern of tyrosine phosphorylation and relationship to malignant transformationF Fazioli
Laboratory of Molecular and Cellular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
Oncogene 8:1335-45. 1993.... - Preferential binding of tumor suppressor p53 to positively or negatively supercoiled DNA involves the C-terminal domainS J Mazur
Laboratory of Molecular Genetics, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA
J Mol Biol 292:241-9. 1999..The preferential binding of p53 is exhibited toward both positively and negatively supercoiled DNA. These observations are consistent with a model in which p53 binds to right-handed or left-handed strand crossings... - Phosphorylation of serine 392 stabilizes the tetramer formation of tumor suppressor protein p53K Sakaguchi
Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Biochemistry 36:10117-24. 1997..Enhancement of tetramer formation through phosphorylation of Ser392, coupled with a DNA-damage-induced increase in its nuclear concentration, could provide a switch that activates p53 as a transcription factor in response to DNA damage... - Cloning and nucleotide sequence of the murine hsp84 cDNA and chromosome assignment of related sequencesS K Moore
Laboratory of Cell Biology, National Cancer Institute, Bethesda, MD 20892
Gene 56:29-40. 1987..This homology did not extend to the 5' - and 3'-untranslated regions. Chromosomal analysis indicated that hsp84-related sequences are on at least three different chromosomes... - Three-dimensional structure of interleukin 8 in solutionG M Clore
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892
Biochemistry 29:1689-96. 1990.... - Phosphorylation at Ser-15 and Ser-392 in mutant p53 molecules from human tumors is altered compared to wild-type p53S J Ullrich
Laboratory of Cell Biology, National Cancer Institute, Bethesda, MD 20892
Proc Natl Acad Sci U S A 90:5954-8. 1993..We suggest that phosphorylation of Ser-15 may depend on the ability of p53 to adopt a wild-type conformation and may contribute to p53's ability to block cell growth... - Inactivation of HIV-1 nucleocapsid protein P7 by pyridinioalkanoyl thioesters. Characterization of reaction products and proposed mechanism of actionV Basrur
Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 275:14890-7. 2000..The results obtained herein demonstrate that PATE compounds can be constructed that selectively target only one of the two zinc fingers of NCp7, thus providing an impetus to pursue development of highly selective zinc finger inhibitors... - Downstream signaling molecules bind to different phosphorylated immunoreceptor tyrosine-based activation motif (ITAM) peptides of the high affinity IgE receptorT Kimura
Laboratory of Immunology, NIDR, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 271:27962-8. 1996..Tyrosine phosphorylation of the ITAM of the gamma subunit recruits and activates Syk, whereas the beta subunit may be important for the Ras signaling pathway... - Linkage of the mouse Hsp84 heat shock protein structural gene to the H-2 complexJ W Romano
Laboratory of Cell Biology, National Cancer Institute, Bethesda, MD 20892
Immunogenetics 29:142-4. 1989 - Complete amino acid sequence of a human monocyte chemoattractant, a putative mediator of cellular immune reactionsE A Robinson
Laboratory of Cell Biology, National Cancer Institute, Bethesda, MD 20892
Proc Natl Acad Sci U S A 86:1850-4. 1989..Thus, GDCF and MDNCF have a similar gross secondary structure because of the loops formed by the clustered disulfides, and their different leukocyte specificities are most likely determined by the large differences in primary sequence... - CD45 regulation of tyrosine phosphorylation and enzyme activity of src family kinasesC M Burns
Laboratory of Immune Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
J Biol Chem 269:13594-600. 1994..Loss of CD45 results, therefore, in a paradoxical hyperphosphorylation of the COOH-terminal tyrosine and increased src family kinase enzymatic activity... - Cloning of a new gene encoding an antigen recognized by melanoma-specific HLA-A24-restricted tumor-infiltrating lymphocytesP F Robbins
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 154:5944-50. 1995..This clone also appeared to represent a transcript of the p15 gene, indicating that this gene may encode the predominant Ag recognized by TIL 1290... - Affinity purification, peptide analysis, and cDNA sequence of the mouse interferon gamma receptorF Cofano
Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
J Biol Chem 265:4064-71. 1990..Mouse IFN-gamma receptor cDNA when inserted in a mammalian shuttle vector and transfected into COS-7 monkey cells was able to direct the expression of specific binding activity for mouse IFN-gamma... - An interferon gamma-regulated protein that binds the interferon-inducible enhancer element of major histocompatibility complex class I genesP H Driggers
Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892
Proc Natl Acad Sci U S A 87:3743-7. 1990..The presence of multiple factors that bind common IFN response motifs may partly account for the complexity and diversity of IFN action as well as IFN-regulated gene expression... - Napsin A, a member of the aspartic protease family, is abundantly expressed in normal lung and kidney tissue and is expressed in lung adenocarcinomasY Chuman
Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
FEBS Lett 462:129-34. 1999..This protease is concluded to have a specific functional role in the normal alveolar epithelium and is a candidate protease for the proteolytic processing of surfactant precursors... - H-2RIIBP (RXR beta) heterodimerization provides a mechanism for combinatorial diversity in the regulation of retinoic acid and thyroid hormone responsive genesM S Marks
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892
EMBO J 11:1419-35. 1992..The results suggest that H-2RIIBP, by virtue of its ability to heterodimerize, enhances combinatorial diversity and versatility in gene regulation mediated by nuclear hormone receptors... - High-resolution solution structure of the double Cys2His2 zinc finger from the human enhancer binding protein MBP-1J G Omichinski
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892
Biochemistry 31:3907-17. 1992.... - Three-dimensional solution structure of the E3-binding domain of the dihydrolipoamide succinyltransferase core from the 2-oxoglutarate dehydrogenase multienzyme complex of Escherichia coliM A Robien
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892
Biochemistry 31:3463-71. 1992..This report presents the first structure of an E3-binding domain from a 2-oxo acid dehydrogenase complex.(ABSTRACT TRUNCATED AT 250 WORDS).. - Mapping of the mouse 86-kDa heat-shock protein expressed gene (Hsp86-1) on chromosome 12 and related genes on chromosomes 3, 4, 9, and 11S K Moore
Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
Genomics 10:1019-29. 1991..An HSP86-related locus specific to NFS/N and C58/J mice, designated Hsp86-ps3, was mapped on Chromosome 9. Also, an HSP86-related locus that was unique to NFS/N mice, designated Hsp86-ps4, was mapped to Chromosome 4... - Phosphorylation of murine p53 at ser-18 regulates the p53 responses to DNA damageC Chao
Division of Biology and Cancer Center, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0322, USA
Proc Natl Acad Sci U S A 97:11936-41. 2000..Additionally, efficient acetylation of the C terminus of p53 in response to DNA damage did not require phosphorylation of murine p53 at Ser-18... - Cloning and expression of the receptor for human urokinase plasminogen activator, a central molecule in cell surface, plasmin dependent proteolysisA L Roldan
University Institute of Microbiology, Copenhagen, Denmark
EMBO J 9:467-74. 1990..The Mr calculated on the basis of the cDNA sequence, approximately 35,000, agrees well with that of the deglycosylated receptor... - Amino acid sequence of a mouse myeloma immunoglobin heavy chain (MOPC 47 A) with a 100-residue deletionE A Robinson
J Biol Chem 254:11418-30. 1979 - TAP-independent presentation of CTL epitopes by Trojan antigensJ Lu
Department of Immunology and Cancer Center, Mayo Clinic and Mayo Graduate School, Rochester, MN 55905, USA
J Immunol 166:7063-71. 2001..We believe that these findings will be of value for the design of CTL-inducing vaccines for the treatment or prevention of infectious and malignant diseases... - T cell activity correlates with oligomeric peptide-major histocompatibility complex binding on T cell surfaceJ Buslepp
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, NC 27599, USA
J Biol Chem 276:47320-8. 2001..However, contrary to previous studies, we see similar half-lives for the pMHC multimers bound to the AHIII12.2 cell surface... - Inhibition of the SH3 domain-mediated binding of Src to the androgen receptor and its effect on tumor growthA Migliaccio
Dipartimento di Patologia Generale, Facolta di Medicina e Chirurgia, II Universita di Napoli, Via L De Crecchio, Napoli, Italia
Oncogene 26:6619-29. 2007..The importance of these results is highlighted by the finding that the peptide strongly inhibits the growth of LNCaP xenografts established in nude mice... - A mouse tumor-specific transplantation antigen is a heat shock-related proteinS J Ullrich
Proc Natl Acad Sci U S A 83:3121-5. 1986..In addition, an anti-Meth A tumor antigen serum that defects the isoforms from a variety of tumors also immunoprecipitates proteins of identical mass and pI from both normal and heat-shocked mouse embryo cells... - DNA-dependent protein kinase is not required for the p53-dependent response to DNA damageG S Jimenez
Gene Expression Laboratory, The Salk Institute, La Jolla, California 92037, USA
Nature 400:81-3. 1999..Our results indicate that DNA-PK activity is not required for cells to mount a p53-dependent response to DNA damage... - Activation of the ATM kinase by ionizing radiation and phosphorylation of p53C E Canman
The Johns Hopkins School of Medicine, Oncology Center, Baltimore, MD 21205, USA
Science 281:1677-9. 1998..These observations, along with the fact that phosphorylation of p53 on serine-15 in response to ionizing radiation is reduced in ataxia telangiectasia cells, suggest that ATM is a protein kinase that phosphorylates p53 in vivo... - The human receptor for urokinase plasminogen activator. NH2-terminal amino acid sequence and glycosylation variantsN Behrendt
Finsen Laboratory, Righospitalet, Copenhagen, Denmark
J Biol Chem 265:6453-60. 1990.... - Partial amino acid sequence of mouse beta2-microglobulinE Appella
Biochem Biophys Res Commun 70:425-30. 1976 - Determination of the secondary structure of interleukin-8 by nuclear magnetic resonance spectroscopyG M Clore
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892
J Biol Chem 264:18907-11. 1989..As a result, the two units of the dimer form a contiguous six-stranded anti-parallel beta-sheet. The secondary structure of IL-8 is similar to that found in the crystal structure of the sequence related protein platelet factor 4... - Purification and biochemical properties of tumor-associated transplantation antigens from methylcholanthrene-induced murine sarcomasG C DuBois
Proc Natl Acad Sci U S A 79:7669-73. 1982..These results indicate that the individually specific transplantation antigens found in chemically induced sarcomas may be the products of a single multigene family or somatic derivatives of a single gene... - The nucleotide sequence of a human cellular thyroid hormone binding protein present in endoplasmic reticulumS Y Cheng
J Biol Chem 262:11221-7. 1987..The isolation of the cDNA clone should allow elucidation of the cellular function of this thyroid hormone binding protein which is present in the endoplasmic reticulum and nuclear envelope... 
PML enhances the regulation of p53 by CK1 in response to DNA damageO Alsheich-Bartok
Department of Immunology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
Oncogene 27:3653-61. 2008..We propose that following DNA damage, PML facilitates Thr18 phosphorylation by recruiting p53 and CK1 into PML nuclear bodies, thereby protecting p53 from inhibition by Mdm2, leading to p53 activation...