E Appella

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Post-translational modifications and activation of p53 by genotoxic stresses
    E Appella
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Eur J Biochem 268:2764-72. 2001
  2. ncbi request reprint Murine 86- and 84-kDa heat shock proteins, cDNA sequences, chromosome assignments, and evolutionary origins
    S K Moore
    Laboratory of Cell Biology, National Cancer Institute, Bethesda, Maryland 20892
    J Biol Chem 264:5343-51. 1989
  3. ncbi request reprint H-2RIIBP expressed from a baculovirus vector binds to multiple hormone response elements
    M S Marks
    Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892
    Mol Endocrinol 6:219-30. 1992
  4. pmc H-2RIIBP, a member of the nuclear hormone receptor superfamily that binds to both the regulatory element of major histocompatibility class I genes and the estrogen response element
    K Hamada
    Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 86:8289-93. 1989
  5. pmc DNA damage activates p53 through a phosphorylation-acetylation cascade
    K Sakaguchi
    Laboratory of Cell Biology, National Cancer Institute NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 12:2831-41. 1998
  6. ncbi request reprint The negative signaling molecule SH2 domain-containing inositol-polyphosphate 5-phosphatase (SHIP) binds to the tyrosine-phosphorylated beta subunit of the high affinity IgE receptor
    T Kimura
    Laboratory of Immunology, NIDR, NCI, National Institutes of Health, Bethesda, Maryland 20892 1188, USA
    J Biol Chem 272:13991-6. 1997
  7. ncbi request reprint NMR structure of a specific DNA complex of Zn-containing DNA binding domain of GATA-1
    J G Omichinski
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892
    Science 261:438-46. 1993
  8. ncbi request reprint Characterization of the mouse 84-kD heat shock protein gene family
    S K Moore
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892
    DNA Cell Biol 9:387-400. 1990
  9. pmc Identification of TRP-2 as a human tumor antigen recognized by cytotoxic T lymphocytes
    R F Wang
    National Cancer Institute, Bethesda, Maryland 20892, USA
    J Exp Med 184:2207-16. 1996
  10. ncbi request reprint Initiation of a G2/M checkpoint after ultraviolet radiation requires p38 kinase
    D V Bulavin
    Division of Basic Science, National Cancer Intitute, National Institutes of Health, Bethesda, MD 20892, USA
    Nature 411:102-7. 2001

Collaborators

Detail Information

Publications51

  1. ncbi request reprint Post-translational modifications and activation of p53 by genotoxic stresses
    E Appella
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Eur J Biochem 268:2764-72. 2001
    ....
  2. ncbi request reprint Murine 86- and 84-kDa heat shock proteins, cDNA sequences, chromosome assignments, and evolutionary origins
    S K Moore
    Laboratory of Cell Biology, National Cancer Institute, Bethesda, Maryland 20892
    J Biol Chem 264:5343-51. 1989
    ..The findings presented herein suggest that HSP86 and HSP84 may have different functions...
  3. ncbi request reprint H-2RIIBP expressed from a baculovirus vector binds to multiple hormone response elements
    M S Marks
    Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892
    Mol Endocrinol 6:219-30. 1992
    ..Taken together, these data demonstrate that H-2RIIBP is capable of binding to hormone response elements of a variety of genes. They suggest that H-2RIIBP may exert a pleiotropic function...
  4. pmc H-2RIIBP, a member of the nuclear hormone receptor superfamily that binds to both the regulatory element of major histocompatibility class I genes and the estrogen response element
    K Hamada
    Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 86:8289-93. 1989
    ..Sequences homologous to the H-2RIIBP gene are widely conserved in the animal kingdom. H-2RIIBP mRNA is expressed in many mouse tissues, in agreement with the distribution of the natural region II factor...
  5. pmc DNA damage activates p53 through a phosphorylation-acetylation cascade
    K Sakaguchi
    Laboratory of Cell Biology, National Cancer Institute NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 12:2831-41. 1998
    ..These results suggest that DNA damage enhances p53 activity as a transcription factor in part through carboxy-terminal acetylation that, in turn, is directed by amino-terminal phosphorylation...
  6. ncbi request reprint The negative signaling molecule SH2 domain-containing inositol-polyphosphate 5-phosphatase (SHIP) binds to the tyrosine-phosphorylated beta subunit of the high affinity IgE receptor
    T Kimura
    Laboratory of Immunology, NIDR, NCI, National Institutes of Health, Bethesda, Maryland 20892 1188, USA
    J Biol Chem 272:13991-6. 1997
    ....
  7. ncbi request reprint NMR structure of a specific DNA complex of Zn-containing DNA binding domain of GATA-1
    J G Omichinski
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892
    Science 261:438-46. 1993
    ..A large number of interactions are observed with the phosphate backbone...
  8. ncbi request reprint Characterization of the mouse 84-kD heat shock protein gene family
    S K Moore
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892
    DNA Cell Biol 9:387-400. 1990
    ..The hsp84 gene family includes at least six different hsp84-related pseudogenes, which arose about 2-3 million years ago...
  9. pmc Identification of TRP-2 as a human tumor antigen recognized by cytotoxic T lymphocytes
    R F Wang
    National Cancer Institute, Bethesda, Maryland 20892, USA
    J Exp Med 184:2207-16. 1996
    ..Like other melamona differentiation antigens, TRP-2 was only expressed in melanoma, melanocytes, and retina, but not in other human tissues tested...
  10. ncbi request reprint Initiation of a G2/M checkpoint after ultraviolet radiation requires p38 kinase
    D V Bulavin
    Division of Basic Science, National Cancer Intitute, National Institutes of Health, Bethesda, MD 20892, USA
    Nature 411:102-7. 2001
    ..We propose that regulation of Cdc25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation...
  11. pmc Identification of a human melanoma antigen recognized by tumor-infiltrating lymphocytes associated with in vivo tumor rejection
    Y Kawakami
    Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 91:6458-62. 1994
    ....
  12. ncbi request reprint High-resolution three-dimensional structure of a single zinc finger from a human enhancer binding protein in solution
    J G Omichinski
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892
    Biochemistry 29:9324-34. 1990
    ....
  13. ncbi request reprint Syk-independent tyrosine phosphorylation and association of the protein tyrosine phosphatases SHP-1 and SHP-2 with the high affinity IgE receptor
    T Kimura
    Laboratory of Immunology, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 159:4426-34. 1997
    ..Thus, unlike other immune cells in which inhibitory molecules are recruited by accessory proteins, Fc epsilonRI bind molecules that both activate and inhibit signal transduction...
  14. ncbi request reprint High-resolution structure of the oligomerization domain of p53 by multidimensional NMR
    G M Clore
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892
    Science 265:386-91. 1994
    ..The implications of the structure of the tetramer for the biological function of p53 are discussed...
  15. pmc A model for melanosome biogenesis based on the purification and analysis of early melanosomes
    T Kushimoto
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 98:10698-703. 2001
    ..These results provide a better understanding of the structural features seen during melanosome biogenesis, and they yield further clues as to the physiological regulation of pigmentation...
  16. pmc Phosphorylation of human p53 by p38 kinase coordinates N-terminal phosphorylation and apoptosis in response to UV radiation
    D V Bulavin
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 18:6845-54. 1999
    ..These results suggest that p38 kinase plays a prominent role in an integrated regulation of N-terminal phosphorylation that regulates p53-mediated apoptosis after UV radiation...
  17. ncbi request reprint p53 regulates the expression of the tumor suppressor gene maspin
    Z Zou
    Department of Surgery, Center for Prostate Disease Research, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    J Biol Chem 275:6051-4. 2000
    ....
  18. ncbi request reprint The ezrin-like family of tyrosine kinase substrates: receptor-specific pattern of tyrosine phosphorylation and relationship to malignant transformation
    F Fazioli
    Laboratory of Molecular and Cellular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
    Oncogene 8:1335-45. 1993
    ....
  19. ncbi request reprint Preferential binding of tumor suppressor p53 to positively or negatively supercoiled DNA involves the C-terminal domain
    S J Mazur
    Laboratory of Molecular Genetics, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA
    J Mol Biol 292:241-9. 1999
    ..The preferential binding of p53 is exhibited toward both positively and negatively supercoiled DNA. These observations are consistent with a model in which p53 binds to right-handed or left-handed strand crossings...
  20. ncbi request reprint Phosphorylation of serine 392 stabilizes the tetramer formation of tumor suppressor protein p53
    K Sakaguchi
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 36:10117-24. 1997
    ..Enhancement of tetramer formation through phosphorylation of Ser392, coupled with a DNA-damage-induced increase in its nuclear concentration, could provide a switch that activates p53 as a transcription factor in response to DNA damage...
  21. ncbi request reprint Cloning and nucleotide sequence of the murine hsp84 cDNA and chromosome assignment of related sequences
    S K Moore
    Laboratory of Cell Biology, National Cancer Institute, Bethesda, MD 20892
    Gene 56:29-40. 1987
    ..This homology did not extend to the 5' - and 3'-untranslated regions. Chromosomal analysis indicated that hsp84-related sequences are on at least three different chromosomes...
  22. ncbi request reprint Three-dimensional structure of interleukin 8 in solution
    G M Clore
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892
    Biochemistry 29:1689-96. 1990
    ....
  23. pmc Phosphorylation at Ser-15 and Ser-392 in mutant p53 molecules from human tumors is altered compared to wild-type p53
    S J Ullrich
    Laboratory of Cell Biology, National Cancer Institute, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 90:5954-8. 1993
    ..We suggest that phosphorylation of Ser-15 may depend on the ability of p53 to adopt a wild-type conformation and may contribute to p53's ability to block cell growth...
  24. ncbi request reprint Inactivation of HIV-1 nucleocapsid protein P7 by pyridinioalkanoyl thioesters. Characterization of reaction products and proposed mechanism of action
    V Basrur
    Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 275:14890-7. 2000
    ..The results obtained herein demonstrate that PATE compounds can be constructed that selectively target only one of the two zinc fingers of NCp7, thus providing an impetus to pursue development of highly selective zinc finger inhibitors...
  25. ncbi request reprint Downstream signaling molecules bind to different phosphorylated immunoreceptor tyrosine-based activation motif (ITAM) peptides of the high affinity IgE receptor
    T Kimura
    Laboratory of Immunology, NIDR, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 271:27962-8. 1996
    ..Tyrosine phosphorylation of the ITAM of the gamma subunit recruits and activates Syk, whereas the beta subunit may be important for the Ras signaling pathway...
  26. ncbi request reprint Linkage of the mouse Hsp84 heat shock protein structural gene to the H-2 complex
    J W Romano
    Laboratory of Cell Biology, National Cancer Institute, Bethesda, MD 20892
    Immunogenetics 29:142-4. 1989
  27. pmc Complete amino acid sequence of a human monocyte chemoattractant, a putative mediator of cellular immune reactions
    E A Robinson
    Laboratory of Cell Biology, National Cancer Institute, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 86:1850-4. 1989
    ..Thus, GDCF and MDNCF have a similar gross secondary structure because of the loops formed by the clustered disulfides, and their different leukocyte specificities are most likely determined by the large differences in primary sequence...
  28. ncbi request reprint CD45 regulation of tyrosine phosphorylation and enzyme activity of src family kinases
    C M Burns
    Laboratory of Immune Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 269:13594-600. 1994
    ..Loss of CD45 results, therefore, in a paradoxical hyperphosphorylation of the COOH-terminal tyrosine and increased src family kinase enzymatic activity...
  29. ncbi request reprint Cloning of a new gene encoding an antigen recognized by melanoma-specific HLA-A24-restricted tumor-infiltrating lymphocytes
    P F Robbins
    Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 154:5944-50. 1995
    ..This clone also appeared to represent a transcript of the p15 gene, indicating that this gene may encode the predominant Ag recognized by TIL 1290...
  30. ncbi request reprint Affinity purification, peptide analysis, and cDNA sequence of the mouse interferon gamma receptor
    F Cofano
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 265:4064-71. 1990
    ..Mouse IFN-gamma receptor cDNA when inserted in a mammalian shuttle vector and transfected into COS-7 monkey cells was able to direct the expression of specific binding activity for mouse IFN-gamma...
  31. pmc An interferon gamma-regulated protein that binds the interferon-inducible enhancer element of major histocompatibility complex class I genes
    P H Driggers
    Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 87:3743-7. 1990
    ..The presence of multiple factors that bind common IFN response motifs may partly account for the complexity and diversity of IFN action as well as IFN-regulated gene expression...
  32. ncbi request reprint Napsin A, a member of the aspartic protease family, is abundantly expressed in normal lung and kidney tissue and is expressed in lung adenocarcinomas
    Y Chuman
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    FEBS Lett 462:129-34. 1999
    ..This protease is concluded to have a specific functional role in the normal alveolar epithelium and is a candidate protease for the proteolytic processing of surfactant precursors...
  33. pmc H-2RIIBP (RXR beta) heterodimerization provides a mechanism for combinatorial diversity in the regulation of retinoic acid and thyroid hormone responsive genes
    M S Marks
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892
    EMBO J 11:1419-35. 1992
    ..The results suggest that H-2RIIBP, by virtue of its ability to heterodimerize, enhances combinatorial diversity and versatility in gene regulation mediated by nuclear hormone receptors...
  34. ncbi request reprint High-resolution solution structure of the double Cys2His2 zinc finger from the human enhancer binding protein MBP-1
    J G Omichinski
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892
    Biochemistry 31:3907-17. 1992
    ....
  35. ncbi request reprint Three-dimensional solution structure of the E3-binding domain of the dihydrolipoamide succinyltransferase core from the 2-oxoglutarate dehydrogenase multienzyme complex of Escherichia coli
    M A Robien
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892
    Biochemistry 31:3463-71. 1992
    ..This report presents the first structure of an E3-binding domain from a 2-oxo acid dehydrogenase complex.(ABSTRACT TRUNCATED AT 250 WORDS)..
  36. ncbi request reprint Mapping of the mouse 86-kDa heat-shock protein expressed gene (Hsp86-1) on chromosome 12 and related genes on chromosomes 3, 4, 9, and 11
    S K Moore
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
    Genomics 10:1019-29. 1991
    ..An HSP86-related locus specific to NFS/N and C58/J mice, designated Hsp86-ps3, was mapped on Chromosome 9. Also, an HSP86-related locus that was unique to NFS/N mice, designated Hsp86-ps4, was mapped to Chromosome 4...
  37. pmc Phosphorylation of murine p53 at ser-18 regulates the p53 responses to DNA damage
    C Chao
    Division of Biology and Cancer Center, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0322, USA
    Proc Natl Acad Sci U S A 97:11936-41. 2000
    ..Additionally, efficient acetylation of the C terminus of p53 in response to DNA damage did not require phosphorylation of murine p53 at Ser-18...
  38. pmc Cloning and expression of the receptor for human urokinase plasminogen activator, a central molecule in cell surface, plasmin dependent proteolysis
    A L Roldan
    University Institute of Microbiology, Copenhagen, Denmark
    EMBO J 9:467-74. 1990
    ..The Mr calculated on the basis of the cDNA sequence, approximately 35,000, agrees well with that of the deglycosylated receptor...
  39. ncbi request reprint Amino acid sequence of a mouse myeloma immunoglobin heavy chain (MOPC 47 A) with a 100-residue deletion
    E A Robinson
    J Biol Chem 254:11418-30. 1979
  40. ncbi request reprint TAP-independent presentation of CTL epitopes by Trojan antigens
    J Lu
    Department of Immunology and Cancer Center, Mayo Clinic and Mayo Graduate School, Rochester, MN 55905, USA
    J Immunol 166:7063-71. 2001
    ..We believe that these findings will be of value for the design of CTL-inducing vaccines for the treatment or prevention of infectious and malignant diseases...
  41. ncbi request reprint T cell activity correlates with oligomeric peptide-major histocompatibility complex binding on T cell surface
    J Buslepp
    Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, NC 27599, USA
    J Biol Chem 276:47320-8. 2001
    ..However, contrary to previous studies, we see similar half-lives for the pMHC multimers bound to the AHIII12.2 cell surface...
  42. ncbi request reprint Inhibition of the SH3 domain-mediated binding of Src to the androgen receptor and its effect on tumor growth
    A Migliaccio
    Dipartimento di Patologia Generale, Facolta di Medicina e Chirurgia, II Universita di Napoli, Via L De Crecchio, Napoli, Italia
    Oncogene 26:6619-29. 2007
    ..The importance of these results is highlighted by the finding that the peptide strongly inhibits the growth of LNCaP xenografts established in nude mice...
  43. pmc A mouse tumor-specific transplantation antigen is a heat shock-related protein
    S J Ullrich
    Proc Natl Acad Sci U S A 83:3121-5. 1986
    ..In addition, an anti-Meth A tumor antigen serum that defects the isoforms from a variety of tumors also immunoprecipitates proteins of identical mass and pI from both normal and heat-shocked mouse embryo cells...
  44. ncbi request reprint DNA-dependent protein kinase is not required for the p53-dependent response to DNA damage
    G S Jimenez
    Gene Expression Laboratory, The Salk Institute, La Jolla, California 92037, USA
    Nature 400:81-3. 1999
    ..Our results indicate that DNA-PK activity is not required for cells to mount a p53-dependent response to DNA damage...
  45. ncbi request reprint Activation of the ATM kinase by ionizing radiation and phosphorylation of p53
    C E Canman
    The Johns Hopkins School of Medicine, Oncology Center, Baltimore, MD 21205, USA
    Science 281:1677-9. 1998
    ..These observations, along with the fact that phosphorylation of p53 on serine-15 in response to ionizing radiation is reduced in ataxia telangiectasia cells, suggest that ATM is a protein kinase that phosphorylates p53 in vivo...
  46. ncbi request reprint The human receptor for urokinase plasminogen activator. NH2-terminal amino acid sequence and glycosylation variants
    N Behrendt
    Finsen Laboratory, Righospitalet, Copenhagen, Denmark
    J Biol Chem 265:6453-60. 1990
    ....
  47. ncbi request reprint Partial amino acid sequence of mouse beta2-microglobulin
    E Appella
    Biochem Biophys Res Commun 70:425-30. 1976
  48. ncbi request reprint Determination of the secondary structure of interleukin-8 by nuclear magnetic resonance spectroscopy
    G M Clore
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892
    J Biol Chem 264:18907-11. 1989
    ..As a result, the two units of the dimer form a contiguous six-stranded anti-parallel beta-sheet. The secondary structure of IL-8 is similar to that found in the crystal structure of the sequence related protein platelet factor 4...
  49. pmc Purification and biochemical properties of tumor-associated transplantation antigens from methylcholanthrene-induced murine sarcomas
    G C DuBois
    Proc Natl Acad Sci U S A 79:7669-73. 1982
    ..These results indicate that the individually specific transplantation antigens found in chemically induced sarcomas may be the products of a single multigene family or somatic derivatives of a single gene...
  50. ncbi request reprint The nucleotide sequence of a human cellular thyroid hormone binding protein present in endoplasmic reticulum
    S Y Cheng
    J Biol Chem 262:11221-7. 1987
    ..The isolation of the cDNA clone should allow elucidation of the cellular function of this thyroid hormone binding protein which is present in the endoplasmic reticulum and nuclear envelope...
  51. doi request reprint PML enhances the regulation of p53 by CK1 in response to DNA damage
    O Alsheich-Bartok
    Department of Immunology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
    Oncogene 27:3653-61. 2008
    ..We propose that following DNA damage, PML facilitates Thr18 phosphorylation by recruiting p53 and CK1 into PML nuclear bodies, thereby protecting p53 from inhibition by Mdm2, leading to p53 activation...