MIRIT ALADJEM

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Predicted functions of MdmX in fine-tuning the response of p53 to DNA damage
    Sohyoung Kim
    Laboratory of Molecular Pharmacology, National Cancer Institute, National Institute of Health, Bethesda, Maryland, United States of America
    PLoS Comput Biol 6:e1000665. 2010
  2. pmc Methylation of histone H3 on lysine 79 associates with a group of replication origins and helps limit DNA replication once per cell cycle
    Haiqing Fu
    Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD, USA
    PLoS Genet 9:e1003542. 2013
  3. pmc A formal MIM specification and tools for the common exchange of MIM diagrams: an XML-Based format, an API, and a validation method
    Augustin Luna
    Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    BMC Bioinformatics 12:167. 2011
  4. ncbi request reprint Replication in context: dynamic regulation of DNA replication patterns in metazoans
    Mirit I Aladjem
    Laboratory of Molecular Pharmacology, National Cancer Institute, National Institutes of Health, Building 37, Room 5056, 37 Convent Drive, Bethesda, Maryland 20892 4255, USA
    Nat Rev Genet 8:588-600. 2007
  5. ncbi request reprint The mammalian beta globin origin of DNA replication
    Mirit I Aladjem
    Laboratory of Molecular Pharmacology, National Cancer Institute, Bethesda, MD, USA
    Front Biosci 9:2540-7. 2004
  6. pmc The replicon revisited: an old model learns new tricks in metazoan chromosomes
    Mirit I Aladjem
    Laboratory of Molecular Pharmacology, DSB, National Cancer Institute, National Institutes of Health, Building 37, Room 5056, 37 Convent Drive, Bethesda, Maryland 20892 4255, USA
    EMBO Rep 5:686-91. 2004
  7. doi request reprint EMBO Conference on Replication and Segregation of Chromosomes, Geilo, Norway, June 16-20. Replication and segregation of chromosomes in the three domains of life: EMBO conference reports common grounds. Meeting report
    MIRIT ALADJEM
    Laboratories of Pharmacology, Center for Cancer Research, NCI, NIH, Bethesda, MD 20892 4260, USA
    Plasmid 61:89-93. 2009
  8. ncbi request reprint Molecular interaction maps--a diagrammatic graphical language for bioregulatory networks
    Mirit I Aladjem
    Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Sci STKE 2004:pe8. 2004
  9. ncbi request reprint Phosphorylation of histone H2AX and activation of Mre11, Rad50, and Nbs1 in response to replication-dependent DNA double-strand breaks induced by mammalian DNA topoisomerase I cleavage complexes
    Takahisa Furuta
    Laboratory of Molecular Pharmacology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892 4255, USA
    J Biol Chem 278:20303-12. 2003
  10. pmc The intra-S-phase checkpoint affects both DNA replication initiation and elongation: single-cell and -DNA fiber analyses
    Jennifer A Seiler
    Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 4255, USA
    Mol Cell Biol 27:5806-18. 2007

Collaborators

Detail Information

Publications33

  1. pmc Predicted functions of MdmX in fine-tuning the response of p53 to DNA damage
    Sohyoung Kim
    Laboratory of Molecular Pharmacology, National Cancer Institute, National Institute of Health, Bethesda, Maryland, United States of America
    PLoS Comput Biol 6:e1000665. 2010
    ..Our study suggests how MdmX may participate in the response of p53 to DNA damage either by increasing dependency of p53 on Mdm2 or by dampening oscillations of p53 activity and presents a model for experimental investigation...
  2. pmc Methylation of histone H3 on lysine 79 associates with a group of replication origins and helps limit DNA replication once per cell cycle
    Haiqing Fu
    Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD, USA
    PLoS Genet 9:e1003542. 2013
    ..These data are consistent with the hypothesis that dimethylated H3K79 associates with some replication origins and marks replicated chromatin during S-phase to prevent re-replication and preserve genomic stability...
  3. pmc A formal MIM specification and tools for the common exchange of MIM diagrams: an XML-Based format, an API, and a validation method
    Augustin Luna
    Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    BMC Bioinformatics 12:167. 2011
    ..A lack of software tools for the notation restricts wider usage of the notation. Development of software is facilitated by a more detailed specification regarding software requirements than has previously existed for the MIM notation...
  4. ncbi request reprint Replication in context: dynamic regulation of DNA replication patterns in metazoans
    Mirit I Aladjem
    Laboratory of Molecular Pharmacology, National Cancer Institute, National Institutes of Health, Building 37, Room 5056, 37 Convent Drive, Bethesda, Maryland 20892 4255, USA
    Nat Rev Genet 8:588-600. 2007
    ....
  5. ncbi request reprint The mammalian beta globin origin of DNA replication
    Mirit I Aladjem
    Laboratory of Molecular Pharmacology, National Cancer Institute, Bethesda, MD, USA
    Front Biosci 9:2540-7. 2004
    ....
  6. pmc The replicon revisited: an old model learns new tricks in metazoan chromosomes
    Mirit I Aladjem
    Laboratory of Molecular Pharmacology, DSB, National Cancer Institute, National Institutes of Health, Building 37, Room 5056, 37 Convent Drive, Bethesda, Maryland 20892 4255, USA
    EMBO Rep 5:686-91. 2004
    ..Here, we review the properties of metazoan replicators, and discuss the genetic and epigenetic factors that determine where and when DNA replication is initiated...
  7. doi request reprint EMBO Conference on Replication and Segregation of Chromosomes, Geilo, Norway, June 16-20. Replication and segregation of chromosomes in the three domains of life: EMBO conference reports common grounds. Meeting report
    MIRIT ALADJEM
    Laboratories of Pharmacology, Center for Cancer Research, NCI, NIH, Bethesda, MD 20892 4260, USA
    Plasmid 61:89-93. 2009
    ..The organizers Kirsten Skarstad and Erik Boye deserve praise for their skillful organization of the meeting, the highlights of which are discussed below...
  8. ncbi request reprint Molecular interaction maps--a diagrammatic graphical language for bioregulatory networks
    Mirit I Aladjem
    Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Sci STKE 2004:pe8. 2004
    ....
  9. ncbi request reprint Phosphorylation of histone H2AX and activation of Mre11, Rad50, and Nbs1 in response to replication-dependent DNA double-strand breaks induced by mammalian DNA topoisomerase I cleavage complexes
    Takahisa Furuta
    Laboratory of Molecular Pharmacology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892 4255, USA
    J Biol Chem 278:20303-12. 2003
    ..These results demonstrate a conserved gammaH2AX response for double-strand breaks induced by replication fork collision. gammaH2AX foci are required for recruiting repair and checkpoint protein complexes to the replication break sites...
  10. pmc The intra-S-phase checkpoint affects both DNA replication initiation and elongation: single-cell and -DNA fiber analyses
    Jennifer A Seiler
    Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 4255, USA
    Mol Cell Biol 27:5806-18. 2007
    ..Together, our study demonstrates that the intra-S-phase checkpoint is exerted by Chk1 not only upon replication initiation but also upon DNA elongation...
  11. ncbi request reprint Cooperative sequence modules determine replication initiation sites at the human beta-globin locus
    Lixin Wang
    Laboratory of Molecular Pharmacology, National Cancer Institute, Bethesda, MD 20892, USA
    Hum Mol Genet 15:2613-22. 2006
    ..These data support a combinatorial model for replicator activity and suggest that the initiation of DNA replication requires interaction between at least two distinct sequence modules...
  12. pmc Network architecture of signaling from uncoupled helicase-polymerase to cell cycle checkpoints and trans-lesion DNA synthesis
    Kurt W Kohn
    Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Cell Cycle 8:2281-99. 2009
    ..In addition, the network architecture disclosed by the hierarchical map, suggested a speculative model for how molecular crowding and the granular localization of network components in the cell nucleus can facilitate function...
  13. pmc Bloom's syndrome helicase and Mus81 are required to induce transient double-strand DNA breaks in response to DNA replication stress
    Tsutomu Shimura
    Laboratory of Molecular Pharmacology, Center for Cancer Research, NCI, NIH, Bethesda, MD 20892 4255, USA
    J Mol Biol 375:1152-64. 2008
    ....
  14. pmc Chromatin challenges during DNA replication: a systems representation
    Kurt W Kohn
    Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 19:1-7. 2008
    ..on the complexities of chromatin replication, thereby providing a tool for system-level comprehension of the effects of genetic mutations, altered gene expression, and pharmacologic intervention...
  15. pmc Depicting combinatorial complexity with the molecular interaction map notation
    Kurt W Kohn
    Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD, USA
    Mol Syst Biol 2:51. 2006
    ..These comparisons may help cell and systems biologists adopt a graphical language that is unambiguous and generally understood...
  16. pmc Replication initiation patterns in the beta-globin loci of totipotent and differentiated murine cells: evidence for multiple initiation regions
    Mirit I Aladjem
    Laboratory of Molecular Pharmacology, National Cancer Institute, Bethesda, Maryland 20892 4255, USA
    Mol Cell Biol 22:442-52. 2002
    ..As this deletion also did not affect the chromatin structure of the locus, we propose that the sequences determining both chromatin structure and replication initiation lie outside the hypersensitive sites removed by the deletion...
  17. ncbi request reprint Werner syndrome protein directly binds to the AAA ATPase p97/VCP in an ATP-dependent fashion
    Fred Eliezer Indig
    Laboratory of Molecular Gerontology, NIA, NIH, Baltimore MD 21224, USA
    J Struct Biol 146:251-9. 2004
    ..Immunohistochemical analysis of various VCP domains and mutated proteins expressed in vitro demonstrated that VCP may contain several hierarchical cellular localization motifs within its domain structure...
  18. pmc Molecular interaction maps of bioregulatory networks: a general rubric for systems biology
    Kurt W Kohn
    Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 17:1-13. 2006
    ..Drawing a MIM diagram, adhering to the rules of notation, imposes a logical discipline that sharpens one's understanding of the structure and function of a network...
  19. ncbi request reprint Chk2 molecular interaction map and rationale for Chk2 inhibitors
    Yves Pommier
    Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892 4255, USA
    Clin Cancer Res 12:2657-61. 2006
    ..Visualizing the regulatory circuits underlying cellular signaling may help identify key regulatory reactions and defects that can serve as targets for anticancer drugs...
  20. ncbi request reprint Preventing gene silencing with human replicators
    Haiqing Fu
    Laboratory of Molecular Pharmacology, NCI, Bethesda, Maryland 20892, USA
    Nat Biotechnol 24:572-6. 2006
    ..Inclusion of functional replicators in gene therapy vectors may provide a tool for stabilizing gene expression patterns...
  21. pmc DNA-PK is involved in repairing a transient surge of DNA breaks induced by deceleration of DNA replication
    Tsutomu Shimura
    Laboratory of Molecular Pharmacology, Center for Cancer Research, NCI, NIH, Bethesda, MD 20892, USA
    J Mol Biol 367:665-80. 2007
    ..These observations suggest that DNA-PK is involved in setting a high threshold for the ATR-Chk1-mediated S-phase checkpoint by promptly repairing DNA breaks that appear immediately following inhibition of DNA replication...
  22. ncbi request reprint Dynamic alterations of replication timing in mammalian cells
    Chii Mei Lin
    Laboratory of Molecular Pharmacology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892 4255, USA
    Curr Biol 13:1019-28. 2003
    ..The genetic and epigenetic determinants of replication timing in mammalian cells have yet to be elucidated...
  23. pmc The human beta-globin replication initiation region consists of two modular independent replicators
    Lixin Wang
    Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, USA
    Mol Cell Biol 24:3373-86. 2004
    ....
  24. pmc Inhibition of histone deacetylase in cancer cells slows down replication forks, activates dormant origins, and induces DNA damage
    Chiara Conti
    Laboratory of Molecular Pharmacology, National Cancer Institute, NIH, Bethesda, Maryland 20892 4255, USA
    Cancer Res 70:4470-80. 2010
    ..Our findings demonstrate that SAHA produces profound alterations in DNA replication that cause DNA damage, establishing a critical link between robust chromatin acetylation and DNA replication in human cancer cells...
  25. pmc The cyclin-dependent kinase inhibitor Dacapo promotes replication licensing during Drosophila endocycles
    Amy Hong
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 26:2071-82. 2007
    ..Finally, genetic interaction studies suggest that dap functions to promote replication licensing in a subset of Drosophila mitotic cycles...
  26. ncbi request reprint Nonclassic functions of human topoisomerase I: genome-wide and pharmacologic analyses
    Ze Hong Miao
    Laboratories of Molecular Pharmacology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, Maryland, USA
    Cancer Res 67:8752-61. 2007
    ..The reported cell lines and approaches described in this article provide new tools to perform detailed functional analyses related to Top1 function...
  27. pmc Prevention of transcriptional silencing by a replicator-binding complex consisting of SWI/SNF, MeCP1, and hnRNP C1/C2
    Liang Huang
    Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Biol 31:3472-84. 2011
    ..These observations suggest that the interaction of LARC complex with replicators plays a role in preventing gene silencing and provides support for a novel, epigenetic mechanism of resistance to methylation inhibitors...
  28. pmc DNA methylation supports intrinsic epigenetic memory in mammalian cells
    Yong Qing Feng
    Department of Medicine, Division of Hematology, Albert Einstein College of Medicine, Bronx, New York, New York, United States of America
    PLoS Genet 2:e65. 2006
    ..We propose that DNA methylation within transgenes serves as an intrinsic epigenetic memory to permanently silence transgenes and prevent their reactivation...
  29. ncbi request reprint Targeted deletion of the chicken beta-globin regulatory elements reveals a cooperative gene silencing activity
    Jin Wang
    Center for Hematologic Malignancies, Oregon Cancer Institute, Department of Medicine, Oregon Health and Science University, Portland, 97239, USA
    J Biol Chem 280:23340-8. 2005
    ..Our results using a chromosomal transfer assay demonstrate an identical silencing function for these regulatory elements, which suggests they function as part of a common silencing pathway or complex...
  30. pmc The human beta-globin locus control region can silence as well as activate gene expression
    Yong Qing Feng
    Division of Hematology, Department of Medicine, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    Mol Cell Biol 25:3864-74. 2005
    ..We also provide evidence that this novel silencing activity is caused by transcriptional interference triggered by activation of transcription in the flanking sequences by the LCR...
  31. pmc Initiation of DNA replication at the human beta-globin 3' enhancer
    Alla Buzina
    Developmental Biology Program, Hospital for Sick Children Toronto, Ontario, Canada
    Nucleic Acids Res 33:4412-24. 2005
    ..We conclude that a mammalian enhancer can cooperate with adjacent sequences to create an efficient replicator module...
  32. ncbi request reprint Candidate DNA replication initiation regions at human trinucleotide repeat disease loci
    Taurai Nenguke
    Program in Molecular and Computational Biology, University of Southern California, Los Angeles, CA 90089 1340, USA
    Hum Mol Genet 12:1021-8. 2003
    ..Comparison with experimental results from model systems suggests that a complex relationship may exist between instability and the proximity and/or orientation of the repeats with respect to an IR...
  33. pmc Circuit diagrams for biological networks
    Kurt W Kohn
    Mol Syst Biol 2:2006.0002. 2006