Defeng Wu

Summary

Affiliation: Mount Sinai School of Medicine
Country: USA

Publications

  1. pmc Alcohol steatosis and cytotoxicity: the role of cytochrome P4502E1 and autophagy
    Defeng Wu
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Free Radic Biol Med 53:1346-57. 2012
  2. doi request reprint Development and properties of HepG2 cells that constitutively express CYP2E1
    Defeng Wu
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, NY, USA
    Methods Mol Biol 447:137-50. 2008
  3. doi request reprint Oxidative stress and alcoholic liver disease
    Defeng Wu
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, New York 10029, USA
    Semin Liver Dis 29:141-54. 2009
  4. pmc Depletion of cytosolic or mitochondrial thioredoxin increases CYP2E1-induced oxidative stress via an ASK-1-JNK1 pathway in HepG2 cells
    Lili Yang
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Free Radic Biol Med 51:185-96. 2011
  5. pmc Cytochrome P450 2E1 potentiates ethanol induction of hypoxia and HIF-1α in vivo
    Xiaodong Wang
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Free Radic Biol Med 63:175-86. 2013
  6. pmc Chronic alcohol-induced liver injury and oxidant stress are decreased in cytochrome P4502E1 knockout mice and restored in humanized cytochrome P4502E1 knock-in mice
    Yongke Lu
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Free Radic Biol Med 49:1406-16. 2010
  7. pmc CYP2E1 enhances ethanol-induced lipid accumulation but impairs autophagy in HepG2 E47 cells
    Defeng Wu
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Biochem Biophys Res Commun 402:116-22. 2010
  8. pmc Hepatotoxicity mediated by pyrazole (cytochrome P450 2E1) plus tumor necrosis factor alpha treatment occurs in c-Jun N-terminal kinase 2-/- but not in c-Jun N-terminal kinase 1-/- mice
    Xiaodong Wang
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Hepatology 54:1753-66. 2011
  9. pmc Activation of ASK-1 and downstream MAP kinases in cytochrome P4502E1 potentiated tumor necrosis factor alpha liver injury
    Defeng Wu
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Free Radic Biol Med 49:348-60. 2010
  10. doi request reprint Role of oxidative stress in alcohol-induced liver injury
    Arthur I Cederbaum
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, Box 1603, One Gustave L Levy Place, New York, NY 10029, USA
    Arch Toxicol 83:519-48. 2009

Detail Information

Publications22

  1. pmc Alcohol steatosis and cytotoxicity: the role of cytochrome P4502E1 and autophagy
    Defeng Wu
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Free Radic Biol Med 53:1346-57. 2012
    ..Inhibition of autophagy promotes binge ethanol induced hepatotoxicity, steatosis and oxidant stress via CYP2E1...
  2. doi request reprint Development and properties of HepG2 cells that constitutively express CYP2E1
    Defeng Wu
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, NY, USA
    Methods Mol Biol 447:137-50. 2008
    ..Methods are described as to how the cell lines were established and maintained, how CYP2E1 is assayed, how cellular viability, mitochondrial function and generation of oxidant stress are determined...
  3. doi request reprint Oxidative stress and alcoholic liver disease
    Defeng Wu
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, New York 10029, USA
    Semin Liver Dis 29:141-54. 2009
    ..Many pathways play a key role in how ethanol induces oxidative stress. This review summarizes some of the leading pathways and discusses the evidence for their contribution to alcohol-induced liver injury...
  4. pmc Depletion of cytosolic or mitochondrial thioredoxin increases CYP2E1-induced oxidative stress via an ASK-1-JNK1 pathway in HepG2 cells
    Lili Yang
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Free Radic Biol Med 51:185-96. 2011
    ..In conclusion, knockdown of TRX-1 or TRX-2 sensitizes cells to CYP2E1-induced oxidant stress partially via ASK-1 and JNK1 signaling pathways. Both TRX-1 and TRX-2 are important for defense against CYP2E1-induced oxidative stress...
  5. pmc Cytochrome P450 2E1 potentiates ethanol induction of hypoxia and HIF-1α in vivo
    Xiaodong Wang
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Free Radic Biol Med 63:175-86. 2013
    ..Blocking HIF-1α activation and actions may have therapeutic implications for protection against ethanol/CYP2E1-induced oxidative stress, steatosis, and liver injury. ..
  6. pmc Chronic alcohol-induced liver injury and oxidant stress are decreased in cytochrome P4502E1 knockout mice and restored in humanized cytochrome P4502E1 knock-in mice
    Yongke Lu
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Free Radic Biol Med 49:1406-16. 2010
    ....
  7. pmc CYP2E1 enhances ethanol-induced lipid accumulation but impairs autophagy in HepG2 E47 cells
    Defeng Wu
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Biochem Biophys Res Commun 402:116-22. 2010
    ..We hypothesize that CYP2E1-induced oxidative stress promotes the accumulation of lipid droplets by ethanol and this may be responsible for the suppression of autophagy in the liver...
  8. pmc Hepatotoxicity mediated by pyrazole (cytochrome P450 2E1) plus tumor necrosis factor alpha treatment occurs in c-Jun N-terminal kinase 2-/- but not in c-Jun N-terminal kinase 1-/- mice
    Xiaodong Wang
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Hepatology 54:1753-66. 2011
    ..Electron microscopy showed altered mitochondrial structure in the jnk2(-/-) and WT mice, but not the jnk1(-/-) mice...
  9. pmc Activation of ASK-1 and downstream MAP kinases in cytochrome P4502E1 potentiated tumor necrosis factor alpha liver injury
    Defeng Wu
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Free Radic Biol Med 49:348-60. 2010
    ..We speculate that similar interactions between CYP2E1 and TNFalpha may be important for alcohol-induced liver injury...
  10. doi request reprint Role of oxidative stress in alcohol-induced liver injury
    Arthur I Cederbaum
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, Box 1603, One Gustave L Levy Place, New York, NY 10029, USA
    Arch Toxicol 83:519-48. 2009
    ..Special emphasis is placed on CYP2E1, which is induced by alcohol and is reactive in metabolizing and activating many hepatotoxins, including ethanol, to reactive products, and in generating ROS...
  11. pmc Cytochrome P4502E1, oxidative stress, JNK, and autophagy in acute alcohol-induced fatty liver
    Lili Yang
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, Box 1603, One Gustave L Levy Place, New York, NY 10029, USA
    Free Radic Biol Med 53:1170-80. 2012
    ..The results show that acute alcohol elevation of CYP2E1, oxidative stress, and activation of JNK interact to lower autophagy and increase lipogenic SREBP resulting in fatty liver...
  12. doi request reprint Role of nitric oxide and nuclear factor-kappaB in the CYP2E1 potentiation of tumor necrosis factor alpha hepatotoxicity in mice
    Defeng Wu
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Free Radic Biol Med 46:480-91. 2009
    ..These effects combine with the blunting of the NF-kappaB activation pathways and the synthesis of protective factors to cause liver injury...
  13. ncbi request reprint Cytochrome P4502E1 sensitizes to tumor necrosis factor alpha-induced liver injury through activation of mitogen-activated protein kinases in mice
    Defeng Wu
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY, USA
    Hepatology 47:1005-17. 2008
    ..Liver mitochondria from PY plus LPS or PY plus TNF-alpha treated mice underwent calcium-dependent, cyclosporine A-sensitive swelling, which was prevented by SB203580 or SP600125...
  14. pmc Synergistic toxicity induced by prolonged glutathione depletion and inhibition of nuclear factor-kappaB signaling in liver cells
    Jose M Jimenez-Lopez
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Toxicol In Vitro 22:106-15. 2008
    ..In summary, inhibition of NF-kappaB sensitizes liver cells to toxicity linked to GSH depletion, probably accelerating the processes of thiol homeostasis deregulation and induction of apoptosis through a mechanism mediated by p38 MAPK...
  15. pmc Ethanol induction of CYP2A5: permissive role for CYP2E1
    Yongke Lu
    Department of Medicine, Division of Liver Diseases, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    Drug Metab Dispos 39:330-6. 2011
    ..The possibility that induction of CYP2E1 is permissive for the induction of CYP2A5 may reflect a new contribution by CYP2E1 to the actions of ethanol...
  16. ncbi request reprint Nitric oxide donors prevent while the nitric oxide synthase inhibitor L-NAME increases arachidonic acid plus CYP2E1-dependent toxicity
    Defeng Wu
    Department of Pharmacology and Biological Chemistry, Box 1603, One Gustave L Levy Place, Mount Sinai School of Medicine, New York, NY 10029, USA
    Toxicol Appl Pharmacol 216:282-92. 2006
    ..These results indicate that NO can be hepatoprotective against CYP2E1-dependent toxicity, preventing AA-induced oxidative stress...
  17. ncbi request reprint Oxidative stress mediated toxicity exerted by ethanol-inducible CYP2E1
    Defeng Wu
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    Toxicol Appl Pharmacol 207:70-6. 2005
    ..HepG2 cell lines overexpressing CYP2E1 may be a valuable model to characterize the biochemical and toxicological properties of CYP2E1...
  18. ncbi request reprint Opposite action of S-adenosyl methionine and its metabolites on CYP2E1-mediated toxicity in pyrazole-induced rat hepatocytes and HepG2 E47 cells
    Defeng Wu
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, NY 10029, USA
    Am J Physiol Gastrointest Liver Physiol 290:G674-84. 2006
    ..In conclusion, contrasting effects of SAMe on CYP2E1 toxicity were observed in pyrazole hepatocytes and E47 cells. In hepatocytes, SAMe protects against CYP2E1 toxicity by a mechanism involving maintaining or elevating GSH levels...
  19. pmc CYP2E1 Sensitizes the Liver to LPS- and TNF α-Induced Toxicity via Elevated Oxidative and Nitrosative Stress and Activation of ASK-1 and JNK Mitogen-Activated Kinases
    Arthur I Cederbaum
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, P O Box 1603, One Gustave L Levy Place, New York, NY 10029, USA
    Int J Hepatol 2012:582790. 2012
    ..We hypothesize that similar interactions occur as a result of ethanol induction of CYP2E1 and TNFα...
  20. ncbi request reprint Alcohol, oxidative stress, and free radical damage
    Defeng Wu
    Department of Pharmacology and Biological Chemistry Mount Sinai School ofMedicine, New York, New York, USA
    Alcohol Res Health 27:277-84. 2003
    ..e., antioxidants). The resulting state of the cell, known as oxidative stress, can lead to cell injury. ROS production and oxidative stress in liver cells play a central role in the development of alcoholic liver disease...
  21. pmc Sulforaphane induces Nrf2 and protects against CYP2E1-dependent binge alcohol-induced liver steatosis
    Richard Zhou
    Depetment of Pharmacology and Systems Therapeutics, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA
    Biochim Biophys Acta 1840:209-18. 2014
    ..The transcription factor Nrf2 regulates the expression of many cytoprotective enzymes which results in cellular protection against a variety of toxins...