Zhen Qiang Pan

Summary

Affiliation: Mount Sinai School of Medicine
Country: USA

Publications

  1. ncbi Nedd8 on cullin: building an expressway to protein destruction
    Zhen Qiang Pan
    Derald H Ruttenberg Cancer Center, The Mount Sinai School of Medicine, New York, NY 10029 6574, USA
    Oncogene 23:1985-97. 2004
  2. ncbi Teaching resources. Nuclear transactivators and repressors
    Zhen Qiang Pan
    Department of Oncological Sciences, Mount Sinai School of Medicine, New York, NY 10029, USA
    Sci STKE 2005:tr18. 2005
  3. ncbi Inactivation of VHL by tumorigenic mutations that disrupt dynamic coupling of the pVHL.hypoxia-inducible transcription factor-1alpha complex
    Felicia Miller
    Department of Oncological Sciences, The Mount Sinai School of Medicine, New York, New York 10029 6574, USA
    J Biol Chem 280:7985-96. 2005
  4. ncbi Proximity-induced activation of human Cdc34 through heterologous dimerization
    Stefan Gazdoiu
    Department of Oncological Sciences, Mount Sinai School of Medicine, New York, NY 10029 6574, USA
    Proc Natl Acad Sci U S A 102:15053-8. 2005
  5. ncbi CUL7: A DOC domain-containing cullin selectively binds Skp1.Fbx29 to form an SCF-like complex
    Dora C Dias
    Derald H Ruttenberg Cancer Center and Department of Human Genetics, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029 6574, USA
    Proc Natl Acad Sci U S A 99:16601-6. 2002
  6. ncbi The Nedd8-conjugated ROC1-CUL1 core ubiquitin ligase utilizes Nedd8 charged surface residues for efficient polyubiquitin chain assembly catalyzed by Cdc34
    Kenneth Wu
    Derald H Ruttenberg Cancer Center, The Mount Sinai School of Medicine, New York, New York 10029 6574, USA
    J Biol Chem 277:516-27. 2002
  7. ncbi Autoubiquitination of the BRCA1*BARD1 RING ubiquitin ligase
    Angus Chen
    Derald H Ruttenberg Cancer Center, The Mount Sinai School of Medicine, New York, New York 10029 6574, USA
    J Biol Chem 277:22085-92. 2002
  8. ncbi DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1
    Kenneth Wu
    Derald H Ruttenberg Cancer Center and Department of Human Genetics, The Mount Sinai School of Medicine, New York, New York 10029 6574, USA
    J Biol Chem 278:28882-91. 2003
  9. ncbi Priming and extending: a UbcH5/Cdc34 E2 handoff mechanism for polyubiquitination on a SCF substrate
    Kenneth Wu
    Department of Oncological Sciences, The Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029 6574, USA
    Mol Cell 37:784-96. 2010
  10. ncbi Human Cdc34 employs distinct sites to coordinate attachment of ubiquitin to a substrate and assembly of polyubiquitin chains
    Stefan Gazdoiu
    Department of Oncological Sciences, The Mount Sinai School of Medicine, New York, NY 10029 6574, USA
    Mol Cell Biol 27:7041-52. 2007

Research Grants

  1. The function and regulation of the cullin family E3 ubiquitin ligases
    Zhen Qiang Pan; Fiscal Year: 2010
  2. The function and regulation of the cullin family E3 ubiquitin ligases
    Zhen Qiang Pan; Fiscal Year: 2009
  3. Regulation of the cullin family E3 ubiquitin ligases
    Zhen Qiang Pan; Fiscal Year: 2007
  4. Studies on ROC1-dependent E3 Ubiquitin Ligases
    Zhen Qiang Pan; Fiscal Year: 2007
  5. Studies on ROC1-dependent Ubiquitin E3 Ligases
    Zhen Qiang Pan; Fiscal Year: 2006
  6. Regulation of the cullin family E3 ubiquitin ligases
    Zhen Qiang Pan; Fiscal Year: 2006
  7. Studies on ROC1-dependent E3 Ubiquitin Ligases
    Zhen Qiang Pan; Fiscal Year: 2010

Collaborators

  • Antonio Sarikas
  • Rong Wang
  • Keith D Wilkinson
  • Toru Ouchi
  • Xinjiang Wang
  • CATHIE PFLEGER
  • Hua Yan
  • Kenneth Wu
  • Matthew J O'Connell
  • Aneel K Aggarwal
  • Chee Gun Lee
  • Christopher D Lima
  • Katherine L B Borden
  • Nikola Pavletich
  • Ning Wei
  • Kosj Yamoah
  • Xinsong Xu
  • Stefan Gazdoiu
  • Jeffrey Jones
  • Amrita Kumar
  • Karina Umanskaya
  • Masha V Poyurovsky
  • Roman Osman
  • Alex Kentsis
  • Celine Huber
  • Georgia Dolios
  • Felicia Miller
  • David Reverter
  • Tudeviin Gan-Erdene
  • Dora C Dias
  • Angus Chen
  • Susan Taylor
  • Kathleen Meyer
  • Malik Keshwani
  • Hisako O Nakajima
  • Dora C Dias-Santagata
  • Shih-Chong Tsai
  • Wuqiang Zhu
  • Pascal J Lafontant
  • Hidehiro Nakajima
  • Cortnie Guerrero
  • Shih Chong Tsai
  • Nadinath Nillegoda
  • Taiji Oashi
  • Yingying Yang
  • Loren J Field
  • Lan Huang
  • Tengguo Li
  • Carol Prives
  • Lauren S Collier-Hyams
  • Huixia Wu
  • Andrew S Neish
  • Dean P Jones
  • Susanne Radke
  • Rosie Monardo
  • Harish Chander
  • Jason M Hansen
  • Doris Germain
  • Christina Priest
  • Vladimir Bermudez
  • Inger Tappin
  • Graeme C M Black
  • Heloisa Dos Santos
  • Ineke van der Burgt
  • Tudeviin Gan Erdene
  • Carlos R Escalante
  • Gabriele Gillessen-Kaesbach
  • Martine Le Merrer
  • Maria Luisa Giovannucci Uzielli
  • Wassim Chemaitilly
  • Arnold Munnich
  • Raja Brauner
  • Valerie Cormier-Daire
  • Dora Dias-Santagata
  • Jerard Hurwitz
  • Andre Megarbane
  • Peter E Clayton
  • Andrea Superti-Furga
  • Anna Glaser
  • Kerra Pearce
  • Andrew Read
  • Nathalie Dagoneau
  • James O'Sullivan
  • Gilles Morin
  • Peter J Scambler
  • Raoul Hennekam
  • Robin Winter
  • Kolli Nagamalleswari
  • Luming Yin
  • James L Manley

Detail Information

Publications23

  1. ncbi Nedd8 on cullin: building an expressway to protein destruction
    Zhen Qiang Pan
    Derald H Ruttenberg Cancer Center, The Mount Sinai School of Medicine, New York, NY 10029 6574, USA
    Oncogene 23:1985-97. 2004
    ..Finally, increasing evidence indicates that removal of Nedd8 from its cullin targets, by the action of COP9 Signalosome and possibly other proteases, plays a significant role in the regulation of cullin-mediated proteolysis...
  2. ncbi Teaching resources. Nuclear transactivators and repressors
    Zhen Qiang Pan
    Department of Oncological Sciences, Mount Sinai School of Medicine, New York, NY 10029, USA
    Sci STKE 2005:tr18. 2005
    ..Recent views on the action of Mediator will also be discussed. Finally, a detailed study will be presented to provide evidence for a role for enhancer-promoter communication in gene activation...
  3. ncbi Inactivation of VHL by tumorigenic mutations that disrupt dynamic coupling of the pVHL.hypoxia-inducible transcription factor-1alpha complex
    Felicia Miller
    Department of Oncological Sciences, The Mount Sinai School of Medicine, New York, New York 10029 6574, USA
    J Biol Chem 280:7985-96. 2005
    ....
  4. ncbi Proximity-induced activation of human Cdc34 through heterologous dimerization
    Stefan Gazdoiu
    Department of Oncological Sciences, Mount Sinai School of Medicine, New York, NY 10029 6574, USA
    Proc Natl Acad Sci U S A 102:15053-8. 2005
    ..Thus, juxtaposition of human Cdc34 activates its catalytic capability, suggesting that the SCF-mediated polyubiquitination reaction may require the conversion of Cdc34 from an inactive monomer to a highly active dimeric form...
  5. ncbi CUL7: A DOC domain-containing cullin selectively binds Skp1.Fbx29 to form an SCF-like complex
    Dora C Dias
    Derald H Ruttenberg Cancer Center and Department of Human Genetics, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029 6574, USA
    Proc Natl Acad Sci U S A 99:16601-6. 2002
    ..Strikingly, CUL7 selectively interacts with Skp1.Fbx29 but not with Skp1.betaTRCP2 or Skp1.Skp2. Thus, CUL7 may define a previously uncharacterized, Fbx29-mediated, and ubiquitin-dependent proteolysis pathway...
  6. ncbi The Nedd8-conjugated ROC1-CUL1 core ubiquitin ligase utilizes Nedd8 charged surface residues for efficient polyubiquitin chain assembly catalyzed by Cdc34
    Kenneth Wu
    Derald H Ruttenberg Cancer Center, The Mount Sinai School of Medicine, New York, New York 10029 6574, USA
    J Biol Chem 277:516-27. 2002
    ..These results suggest that Nedd8 charged surface residues mediate the activation of ROC1-CUL1 to specifically support Cdc34-catalyzed ubiquitin polymerization...
  7. ncbi Autoubiquitination of the BRCA1*BARD1 RING ubiquitin ligase
    Angus Chen
    Derald H Ruttenberg Cancer Center, The Mount Sinai School of Medicine, New York, New York 10029 6574, USA
    J Biol Chem 277:22085-92. 2002
    ..These results raise the possibility that BRCA1*BARD1 acts to assemble non-lysine 48-linked polyubiquitin chains that may serve as part of a signaling platform required for coordinating DNA repair-related events...
  8. ncbi DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1
    Kenneth Wu
    Derald H Ruttenberg Cancer Center and Department of Human Genetics, The Mount Sinai School of Medicine, New York, New York 10029 6574, USA
    J Biol Chem 278:28882-91. 2003
    ..These results suggest a unique role for DEN1 in regulating the modification of cullins by Nedd8...
  9. ncbi Priming and extending: a UbcH5/Cdc34 E2 handoff mechanism for polyubiquitination on a SCF substrate
    Kenneth Wu
    Department of Oncological Sciences, The Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029 6574, USA
    Mol Cell 37:784-96. 2010
    ....
  10. ncbi Human Cdc34 employs distinct sites to coordinate attachment of ubiquitin to a substrate and assembly of polyubiquitin chains
    Stefan Gazdoiu
    Department of Oncological Sciences, The Mount Sinai School of Medicine, New York, NY 10029 6574, USA
    Mol Cell Biol 27:7041-52. 2007
    ..These findings support the hypothesis that human Cdc34 S95 and E108/E112 are required to position the donor Ub optimally for catalysis, in a manner that might depend on E2 dimerization...
  11. ncbi Identification of the degradation determinants of insulin receptor substrate 1 for signaling cullin-RING E3 ubiquitin ligase 7-mediated ubiquitination
    Xinsong Xu
    Department of Oncological Sciences, The Mount Sinai School of Medicine, New York, NY 10029 6574, USA
    J Biol Chem 287:40758-66. 2012
    ..Negative feedback regulation of insulin signaling involves ubiquitin-dependent degradation of insulin receptor substrate 1 (IRS1)...
  12. ncbi The Mdm2 RING domain C-terminus is required for supramolecular assembly and ubiquitin ligase activity
    Masha V Poyurovsky
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    EMBO J 26:90-101. 2007
    ..We further show that the Mdm2 C-terminus is involved in intramolecular interactions and can set up a platform for direct protein-protein interactions with the E2...
  13. ncbi Structure of a complex between Nedd8 and the Ulp/Senp protease family member Den1
    David Reverter
    Structural Biology Program, Sloan Kettering Institute, New York, NY 10021, USA
    J Mol Biol 345:141-51. 2005
    ....
  14. ncbi Skp2B stimulates mammary gland development by inhibiting REA, the repressor of the estrogen receptor
    Karina Umanskaya
    Department of Medicine, Division of Hematology Oncology, Mount Sinai School of Medicine, One Gustave L Levy Place, Box 1178, New York, NY 10029, USA
    Mol Cell Biol 27:7615-22. 2007
    ..These results indicate that the observed expression of Skp2B in breast cancer does contribute to tumorigenesis at least in part by modulating the activity of the estrogen receptor...
  15. ncbi Mono-ubiquitination drives nuclear export of the human DCN1-like protein hDCNL1
    Kenneth Wu
    Department of Oncological Sciences, The Mount Sinai School of Medicine, New York, New York 10029 6574, USA
    J Biol Chem 286:34060-70. 2011
    ....
  16. ncbi The cullin7 E3 ubiquitin ligase: a novel player in growth control
    Antonio Sarikas
    Department of Oncological Sciences, The Mount Sinai School of Medicine, New York, New York 10029, USA
    Cell Cycle 7:3154-61. 2008
    ....
  17. ncbi Autoinhibitory regulation of SCF-mediated ubiquitination by human cullin 1's C-terminal tail
    Kosj Yamoah
    Department of Oncological Sciences, Mount Sinai School of Medicine, New York, NY 10029 6574, USA
    Proc Natl Acad Sci U S A 105:12230-5. 2008
    ..We propose that SCF may be subject to autoinhibitory regulation, in which Nedd8 conjugation acts as a molecular switch to drive the E3 into an active state by diminishing the inhibitory ECTD x ROC1 interaction...
  18. ncbi The CUL7 E3 ubiquitin ligase targets insulin receptor substrate 1 for ubiquitin-dependent degradation
    Xinsong Xu
    Department of Oncological Sciences, The Mount Sinai School of Medicine, New York, NY 10029 6574, USA
    Mol Cell 30:403-14. 2008
    ..Taken together, our findings demonstrate a key role for the CUL7 E3 in targeting IRS-1 for degradation, a process that may contribute to the regulation of cellular senescence...
  19. ncbi In vitro cleavage of Nedd8 from cullin 1 by COP9 signalosome and deneddylase 1
    Kosj Yamoah
    Department of Oncological Sciences, Mount Sinai School of Medicine, New York, NY 10029-6574, USA
    Methods Enzymol 398:509-22. 2005
    ..It also describes the development of cell-free systems for cleavage of the Nedd8-cullin 1 isopeptide bond formed in vitro or in vivo...
  20. ncbi Commensal bacteria modulate cullin-dependent signaling via generation of reactive oxygen species
    Amrita Kumar
    Epithelial Pathobiology Unit, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, USA
    EMBO J 26:4457-66. 2007
    ....
  21. ncbi A targeted proteomic analysis of the ubiquitin-like modifier nedd8 and associated proteins
    Jeffrey Jones
    Departments of Physiology and Biophysics and Developmental and Cell Biology, University of California, Irvine, California 92697, USA
    J Proteome Res 7:1274-87. 2008
    ..The vast amount of proteomic information obtained here can provide clues on the biological role of Nedd8 and lay the foundation for an in-depth analysis of the regulation of the Nedd8 pathway...
  22. ncbi Identification and characterization of DEN1, a deneddylase of the ULP family
    Tudeviin Gan-Erdene
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 278:28892-900. 2003
    ..This selectivity was unexpected as DEN1 is most closely related to enzymes that catalyze desumoylation. This observation expands to four the number of DUB families with members that can process the C terminus of Nedd8...
  23. ncbi Identification of mutations in CUL7 in 3-M syndrome
    Celine Huber
    Universite Paris Descartes, Faculte de Medecine, INSERM, AP HP, Hopital Necker Enfants Malades, U393 and Department of Medical Genetics, 149 rue de Sèvres 75015, Paris, France
    Nat Genet 37:1119-24. 2005
    ..These results suggest that impaired ubiquitination may have a role in the pathogenesis of intrauterine growth retardation in humans...

Research Grants17

  1. The function and regulation of the cullin family E3 ubiquitin ligases
    Zhen Qiang Pan; Fiscal Year: 2010
    ....
  2. The function and regulation of the cullin family E3 ubiquitin ligases
    Zhen Qiang Pan; Fiscal Year: 2009
    ....
  3. Regulation of the cullin family E3 ubiquitin ligases
    Zhen Qiang Pan; Fiscal Year: 2007
    ..We propose to determine the molecular identity of this novel activity, which may prove to be yet another critical regulator of the pVHL/HIF-1 pathway. ..
  4. Studies on ROC1-dependent E3 Ubiquitin Ligases
    Zhen Qiang Pan; Fiscal Year: 2007
    ..Our project is to develop molecular and cellular tools to probe for various parts on SCF, NeddS, Cdc34 and DCN-1, and to determine how these parts are put together to function. ..
  5. Studies on ROC1-dependent Ubiquitin E3 Ligases
    Zhen Qiang Pan; Fiscal Year: 2006
    ....
  6. Regulation of the cullin family E3 ubiquitin ligases
    Zhen Qiang Pan; Fiscal Year: 2006
    ..We propose to determine the molecular identity of this novel activity, which may prove to be yet another critical regulator of the pVHL/HIF-1 pathway. ..
  7. Studies on ROC1-dependent E3 Ubiquitin Ligases
    Zhen Qiang Pan; Fiscal Year: 2010
    ..Our project is to develop molecular and cellular tools to probe for various parts on SCF, NeddS, Cdc34 and DCN-1, and to determine how these parts are put together to function. ..