Research Topics
Genomes and Genes
| Miriam MeradSummaryAffiliation: Mount Sinai School of Medicine Country: USA Publications
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Publications
The dendritic cell lineage: ontogeny and function of dendritic cells and their subsets in the steady state and the inflamed settingMiriam Merad
Department of Oncological Sciences, Mount Sinai Medical School, New York, NY 10029, USA
Annu Rev Immunol 31:563-604. 2013..This review discusses major advances in our understanding of the regulation of DC lineage commitment, differentiation, diversification, and function in situ...
Depletion of host Langerhans cells before transplantation of donor alloreactive T cells prevents skin graft-versus-host diseaseMiriam Merad
Department of Pathology, Stanford University School of Medicine, Palo Alto, California 94304, USA
Nat Med 10:510-7. 2004....- Dendritic cell homeostasis and trafficking in transplantationMiriam Merad
Department of Gene and Cell Medicine, Mount Sinai Medical School, 1425 Madison Avenue, New York, NY 10029, USA
Trends Immunol 28:353-9. 2007..Here, we review studies on DC homeostasis and trafficking after transplantation, and examine the role of the host and graft DC in post-transplant immune responses. We also discuss the therapeutic implications of these studies... - Origin, homeostasis and function of Langerhans cells and other langerin-expressing dendritic cellsMiriam Merad
Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
Nat Rev Immunol 8:935-47. 2008..In this Review we describe new developments in the understanding of the biology of LCs and other langerin(+) DCs and discuss the challenges that remain in identifying the role of different DC subsets in tissue immunity... 
Dendritic cell homeostasisMiriam Merad
Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
Blood 113:3418-27. 2009..We furthermore highlight how knowledge of these maintenance mechanisms might impact on understanding of DC malignancies as well as posttransplant immune reactions and their respective therapies...- Langerhans cells at the interface of medicine, science, and industryMiriam Merad
Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
J Invest Dermatol 128:251-5. 2008 - The origin and development of nonlymphoid tissue CD103+ DCsFlorent Ginhoux
Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
J Exp Med 206:3115-30. 2009..Our results reveal that nonlymphoid tissue CD103(+) DCs and lymphoid organ CD8(+) DCs derive from the same precursor and follow a related differentiation program... - Origin of the lamina propria dendritic cell networkMilena Bogunovic
Department of Gene and Cell Medicine, Mount Sinai School of Medicine, 1425 Madison Avenue, New York, NY 10029, USA
Immunity 31:513-25. 2009..Altogether, these results underline the diverse origin of the lamina propria DC network and identify mucosal DCs that arise from pre-DCs as key sentinels of the gut immune system... - GM-CSF controls nonlymphoid tissue dendritic cell homeostasis but is dispensable for the differentiation of inflammatory dendritic cellsMelanie Greter
Department of Oncological Sciences, Mount Sinai School of Medicine, New York, NY 10029, USA
Immunity 36:1031-46. 2012..Thus, Csf-2 is important in vaccine-induced CD8(+) T cell immunity through the regulation of nonlymphoid tissue DC homeostasis rather than control of inflammatory DCs in vivo... - Gene-expression profiles and transcriptional regulatory pathways that underlie the identity and diversity of mouse tissue macrophagesEmmanuel L Gautier
Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, New York, New York, USA
Nat Immunol 13:1118-28. 2012..We further identified how these transcripts and the proteins they encode facilitated distinguishing macrophages from dendritic cells... - Adrenergic nerves govern circadian leukocyte recruitment to tissuesChristoph Scheiermann
Ruth L and David S Gottesman Institute for Stem Cell Biology and Regenerative Medicine, Albert Einstein College of Medicine, New York, NY 10461, USA
Immunity 37:290-301. 2012..These data provide unique insights in the leukocyte adhesion cascade and the potential for time-based therapeutics for transplantation and inflammatory diseases... - Blood-derived dermal langerin+ dendritic cells survey the skin in the steady stateFlorent Ginhoux
Department of Gene and Cell Medicine, Mount Sinai School of Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
J Exp Med 204:3133-46. 2007..We propose that this is an important and previously unappreciated element of immunosurveillance that needs to be taken into account in the design of novel vaccine strategies... - Immature monocytes acquire antigens from other cells in the bone marrow and present them to T cells after maturing in the peripheryFrank Tacke
Department of Gene and Cell Medicine, Icahn Research Institute, Mount Sinai School of Medicine, New York, NY 10029, USA
J Exp Med 203:583-97. 2006..These data reveal that immature monocytes unexpectedly sample antigen from the bone marrow environment and that they can present these antigens after they leave the bone marrow... - Fate mapping analysis reveals that adult microglia derive from primitive macrophagesFlorent Ginhoux
Department of Gene and Cell Medicine and the Immunology Institute, Mount Sinai School of Medicine, 1425 Madison Avenue, New York, NY 10029, USA
Science 330:841-5. 2010..These results identify microglia as an ontogenically distinct population in the mononuclear phagocyte system and have implications for the use of embryonically derived microglial progenitors for the treatment of various brain disorders... - Blood monocyte subsets differentially give rise to CD103+ and CD103- pulmonary dendritic cell populationsClaudia Jakubzick
Department of Gene and Cell Medicine, Icahn Research Institute, Mount Sinai School of Medicine, New York, NY 10029, USA
J Immunol 180:3019-27. 2008..Overall, these data demonstrate that the two circulating subsets of monocytes give rise to distinct tissue DC populations... - In vivo manipulation of dendritic cells overcomes tolerance to unmodified tumor-associated self antigens and induces potent antitumor immunityFumiyoshi Okano
Department of Pathology, Stanford University School of Medicine, Palo Alto, CA 94304, USA
J Immunol 174:2645-52. 2005..These findings demonstrate that unmodified tumor-associated self Ags can be targeted to DCs in vivo to induce potent systemic antitumor immunity... - Flk2+ myeloid progenitors are the main source of Langerhans cellsInes Mende
Stanford Blood Center, 3373 Hillview Ave, Palo Alto, CA 94304, USA
Blood 107:1383-90. 2006..These results indicate that LCs are derived mainly from myeloid progenitors and are dependent on Flt3-ligand for their development... - Systemic analysis of PPARγ in mouse macrophage populations reveals marked diversity in expression with critical roles in resolution of inflammation and airway immunityEmmanuel L Gautier
Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, New York, NY 10029, USA
J Immunol 189:2614-24. 2012..In addition, this work identifies specific macrophage populations as potential targets for the anti-inflammatory actions of PPARγ agonists... - Lymph-migrating, tissue-derived dendritic cells are minor constituents within steady-state lymph nodesClaudia Jakubzick
Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
J Exp Med 205:2839-50. 2008..Instead, our analysis suggests that nonlymphoid organ DCs comprise a major population of DCs within lymph nodes only after introduction of an inflammatory stimulus... - Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell nicheAndrew Chow
Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
J Exp Med 208:261-71. 2011..Thus, strategies that target BM MΦ hold the potential to augment stem cell yields in patients that mobilize HSCs/progenitors poorly... - Identification of a radio-resistant and cycling dermal dendritic cell population in mice and menMilena Bogunovic
Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
J Exp Med 203:2627-38. 2006..Given the role of residual host tissue DCs in transplant immune reactions, these results suggest that dermal DC homeostasis may contribute to the development of cutaneous graft-versus-host disease in clinical transplantation... - Cross-presenting CD103+ dendritic cells are protected from influenza virus infectionJulie Helft
Department of Oncological Sciences, Mount Sinai School of Medicine, New York, New York 10029, USA
J Clin Invest 122:4037-47. 2012..These results show that efficient cross-priming by migratory lung DCs is coupled to the acquisition of an anti-viral status, which is dependent on the type I IFN signaling pathway... - Mammalian target of rapamycin controls dendritic cell development downstream of Flt3 ligand signalingTaheri Sathaliyawala
Department of Microbiology and Immunology, Columbia University Medical Center, New York, NY 10032, USA
Immunity 33:597-606. 2010..Thus, PI3K-mTOR signaling downstream of Flt3L controls DC development, and its restriction by Pten ensures optimal DC pool size and subset composition... - B cell-driven lymphangiogenesis in inflamed lymph nodes enhances dendritic cell mobilizationVeronique Angeli
Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
Immunity 24:203-15. 2006..Knowledge that DC migration from the periphery is augmented by B cell-dependent signals reveals new potential strategies to increase DC migration during vaccination... - Induction of potent antitumor immunity by in situ targeting of intratumoral DCsKatsuyoshi Furumoto
Department of Pathology, Stanford University School of Medicine, Palo Alto, California, USA
J Clin Invest 113:774-83. 2004.... - The sphingosine 1-phosphate receptor 1 causes tissue retention by inhibiting the entry of peripheral tissue T lymphocytes into afferent lymphaticsLevi G Ledgerwood
Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
Nat Immunol 9:42-53. 2008..Thus, the increased sphingosine 1-phosphate present in inflamed peripheral tissues may induce T cell retention and suppress T cell egress... - Origin and functional heterogeneity of non-lymphoid tissue dendritic cells in miceJulie Helft
Department of Gene and Cell Medicine and Mount Sinai School of Medicine, New York, NY, USA
Immunol Rev 234:55-75. 2010..We also review recent results underlining the functional specialization of tissue DCs and discuss the potential implications of these findings in tissue immunity and in the development of novel vaccine strategies... - Pretransplant CSF-1 therapy expands recipient macrophages and ameliorates GVHD after allogeneic hematopoietic cell transplantationDaigo Hashimoto
Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
J Exp Med 208:1069-82. 2011..This study establishes the unexpected key role of host macrophages in inhibiting GVHD and identifies CSF-1 as a potential prophylactic therapy to limit acute GVHD after allo-HCT in the clinic... - Notch2 receptor signaling controls functional differentiation of dendritic cells in the spleen and intestineKanako L Lewis
Department of Microbiology and Immunology, Columbia University Medical Center, New York, NY 10032, USA
Immunity 35:780-91. 2011..Thus, Notch2 is a common differentiation signal for T cell-priming CD11b(+) DC subsets in the spleen and intestine... - Developmental origin of interferon-alpha-producing dendritic cells from hematopoietic precursorsHolger Karsunky
Department of Pathology, Stanford University School of Medicine, Stanford, Calif, USA
Exp Hematol 33:173-81. 2005.... - Langerhans cells renew in the skin throughout life under steady-state conditionsMiriam Merad
Department of Pathology, Stanford University School of Medicine, Stanford, CA 94304, USA
Nat Immunol 3:1135-41. 2002..These data indicate that under steady-state conditions, LCs are maintained locally, but inflammatory changes in the skin result in their replacement by blood-borne LC progenitors... - Dendritic cell and macrophage heterogeneity in vivoDaigo Hashimoto
Department of Oncological Sciences, 1425 Madison Avenue, Mount Sinai School of Medicine, New York, NY 10029, USA
Immunity 35:323-35. 2011..We also emphasize the need to further understand the functional heterogeneity of the tissue DC and macrophage lineages to better comprehend the complex role of these cells in tissue homeostasis and immunity... - Antigen-presenting cell-derived complement modulates graft-versus-host diseaseWing Hong Kwan
Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
J Clin Invest 122:2234-8. 2012..Our data mechanistically link APC-derived complement to T cell-mediated GvHD and support complement inhibition as a therapeutic strategy for GvHD in humans... - Deciphering the transcriptional network of the dendritic cell lineageJennifer C Miller
Immunology Institute, Mount Sinai School of Medicine, New York, New York, USA
Nat Immunol 13:888-99. 2012..We also identified a transcriptional program expressed specifically during the steady-state migration of tissue DCs to the draining lymph nodes that may control tolerance to self tissue antigens... - Harnessing dendritic cells to improve allogeneic hematopoietic cell transplantation outcomeDaigo Hashimoto
Department of Gene and Cell Medicine, Mount Sinai School of Medicine, 1425 Madison Avenue, New York, NY 10029, USA
Semin Immunol 23:50-7. 2011..In this review, we summarize recent progress and potential new therapeutic avenues using dendritic cell-based strategies to improve allogeneic HCT outcome... - Specialized role of migratory dendritic cells in peripheral tolerance inductionJuliana Idoyaga
Laboratory of Cellular Physiology and Immunology and Chris Browne Center for Immunology and Immune Diseases, The Rockefeller University, New York, New York, USA
J Clin Invest 123:844-54. 2013..These results provide a rationale for the development of novel therapies targeting migratory DCs for the treatment of autoimmune diseases... - Tissue-Resident Macrophages Self-Maintain Locally throughout Adult Life with Minimal Contribution from Circulating MonocytesDaigo Hashimoto
Department of Oncological Sciences and Tisch Cancer Institute, Critical Care and Sleep Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA Immunology Institute, Critical Care and Sleep Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
Immunity 38:792-804. 2013..Collectively, these results indicate that tissue-resident macrophages and circulating monocytes should be classified as mononuclear phagocyte lineages that are independently maintained in the steady state... - Dendritic cell regulation of carbon tetrachloride-induced murine liver fibrosis regressionJingjing Jiao
Division of Liver Diseases, Department of Medicine, Mount Sinai School of Medicine, New York, NY, USA
Hepatology 55:244-55. 2012..The results also suggest that Flt3L treatment during fibrosis resolution merits evaluation to accelerate regression of advanced liver fibrosis... - MHC class I/peptide transfer between dendritic cells overcomes poor cross-presentation by monocyte-derived APCs that engulf dying cellsChunfeng Qu
Department of Gene and Cell Medicine and Institute for Immunology, Icahn Medical Institute, Mount Sinai School of Medicine, New York, NY 10029, USA
J Immunol 182:3650-9. 2009..These data reveal a way in which migratory monocyte-derived DCs and other DCs, like lymph node resident DCs, both mediate cross-presentation... - Ontogeny of Lagerhans cells and graft versus host diseaseMiriam Merad
Center for Gene Therapy, Department of Medicine, Mount Sinai School of Medicine, 1425 Madison Avenue, New York, NY 10029, USA
Adv Exp Med Biol 560:115-23. 2005 - CD169(+) macrophages provide a niche promoting erythropoiesis under homeostasis and stressAndrew Chow
1 Ruth L and David S Gottesman Institute for Stem Cell and Regenerative Medicine Research, Albert Einstein College of Medicine, Bronx, New York, USA 2 Department of Oncological Sciences, Mount Sinai School of Medicine, New York, New York, USA 3 Department of Medicine, Mount Sinai School of Medicine, New York, New York, USA
Nat Med 19:429-36. 2013..These results indicate that CD169(+) macrophages promote late erythroid maturation and that modulation of the macrophage compartment may be a new strategy to treat erythropoietic disorders... - R-Ras is required for murine dendritic cell maturation and CD4+ T-cell primingGobind Singh
Department of Oncological Sciences, Mount Sinai School of Medicine, New York, NY, USA
Blood 119:1693-701. 2012..Altogether, these findings provide the first demonstration of a role for R-Ras in cell-mediated immunity and further expand on the complexity of small G-protein signaling in DCs... - Studying the mononuclear phagocyte system in the molecular ageAndrew Chow
Department of Oncological Sciences and Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
Nat Rev Immunol 11:788-98. 2011..The purpose of this manuscript is to review the tools that are currently available and those that are under development to study the origin and function of mononuclear phagocytes... - Dendritic cells in transplantation and immune-based therapiesJames W Young
Department of Medicine, Memorial Sloan Kettering Cancer Center, Weill Medical College of Cornell University, New York, New York 10021, USA
Biol Blood Marrow Transplant 13:23-32. 2007..The use of defined DC subtypes to stimulate both innate and adaptive immunity, either in combination or in a prime-boost vaccine sequence, may prove most useful clinically by harnessing both effector cell compartments... - Langerhans cells arise from monocytes in vivoFlorent Ginhoux
Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
Nat Immunol 7:265-73. 2006..These results identify Gr-1(hi) monocytes as the direct precursors for LCs in vivo and establish the importance of the CSF-1 receptor in this process... - The receptor tyrosine kinase Flt3 is required for dendritic cell development in peripheral lymphoid tissuesClaudia Waskow
Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10065, USA
Nat Immunol 9:676-83. 2008..In contrast, Flt3 was essential to the regulation of homeostatic DC development in the spleen, where it was needed to maintain normal numbers of DCs by controlling their division in the periphery... - Isolation of cutaneous dendritic cellsJulie Helft
Department of Gene and Cell Medicine and the Immunology Institute, Mount Sinai School of Medicine, New York, NY, USA
Methods Mol Biol 595:231-3. 2010..This protocol describes how to isolate cutaneous dendritic cells from mouse ears for flow cytometry analysis and functional assay studies... - Decoding dendritic cell function through module and network analysisGaurav Pandey
Institute for Genomics and Multiscale Biology and Department of Genetics and Genomic Sciences, Mount Sinai School of Medicine, New York, NY 10029, USA
J Immunol Methods 387:71-80. 2013..In addition to recapitulating genes known to regulate the functions of these subsets, these networks reveal several novel genes and interactions that might have important roles in DC biology... - Melanoma immunotherapyShanthi Sivendran
Mount Sinai School of Medicine, New York, NY, USA
Mt Sinai J Med 77:620-42. 2010..Novel strategies to induce the immune system to attack melanomas are reviewed. In the future, it is envisioned that immunotherapy will have further application in combination with cytotoxic and targeted therapies... - Mononuclear phagocyte diversity in the intestineMilena Bogunovic
The Immunology Institute, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
Immunol Res 54:37-49. 2012.... - Expression of the chemokine binding protein M3 promotes marked changes in the accumulation of specific leukocytes subsets within the intestineLimin Shang
Immunology Institute, Mount Sinai School of Medicine, New York, New York, USA
Gastroenterology 137:1006-18, 1018.e1-3. 2009..We analyzed the differential expression of chemokines in distinct segments of the intestine and investigated the importance of chemokines for the distribution of leukocytes in the intestine during homeostatic and inflammatory conditions... - Longitudinal tracking of human dendritic cells in murine models using magnetic resonance imagingKaren C Briley-Saebo
Department of Radiology, Mount Sinai School of Medicine, New York, New York 10029 6574, USA
Magn Reson Med 64:1510-9. 2010..This study suggests that magnetic resonance imaging may be used to longitudinally track Feridex-labeled human dendritic cells for up to 2 weeks after injection... - In vivo manipulation of dendritic cells to induce therapeutic immunityMiriam Merad
Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA
Blood 99:1676-82. 2002..Natural killer cells also contributed to tumor protection. These results show that dendritic cells can be loaded with antigen and activated, in situ, and provide the basis for dendritic cell- targeted clinical strategies... - Dendritic cells in alcoholic liver injury and fibrosisCostica Aloman
Division of Liver Diseases, Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
Alcohol Clin Exp Res 35:776-81. 2011..Understanding the mechanism by which DC modulate liver function after alcohol consumption may help uncover novel therapeutic strategies for the treatment of these conditions... - Exosomes as potent cell-free peptide-based vaccine. II. Exosomes in CpG adjuvants efficiently prime naive Tc1 lymphocytes leading to tumor rejectionNathalie Chaput
, , Department of Clinical Biology, Institut Gustave Roussy, Villejuif, France
J Immunol 172:2137-46. 2004..CpG adjuvants appear to be ideal adjuvants for exosome-based cancer vaccines... - Flt3 ligand regulates dendritic cell development from Flt3+ lymphoid and myeloid-committed progenitors to Flt3+ dendritic cells in vivoHolger Karsunky
Institute for Research in Biomedicine IRB, Via Vincenzo Vela 6, CH 6500 Bellinzona, Switzerland
J Exp Med 198:305-13. 2003.... - Dendritic cell genealogy: a new stem or just another branch?Miriam Merad
Nat Immunol 8:1199-201. 2007
Research Grants
- Homeostasis of dendritic cells and GVHDMiriam Merad; Fiscal Year: 2010....
- Ontogeny of Gut Dendritic Cells in Steady State and Inflamed SettingsMiriam Merad; Fiscal Year: 2010..Finally, our finding that induction of DC chimerism is critical to prevent GVHD leads us, in aim 3, to determine the nature of the circulating precursor that repopulates DCs in a clinically relevant model for allo-BMT. ..
- Homeostasis of dendritic cells and GVHDMiriam Merad; Fiscal Year: 2009....
- Ontogeny of Gut Dendritic Cells in Steady State and Inflamed SettingsMiriam Merad; Fiscal Year: 2009..Finally, our finding that induction of DC chimerism is critical to prevent GVHD leads us, in aim 3, to determine the nature of the circulating precursor that repopulates DCs in a clinically relevant model for allo-BMT. ..
- Homeostasis of dendritic cells and GVHDMiriam Merad; Fiscal Year: 2007..These studies should provide valuable insights into LC regulation of skin immune responses and may lead to improved prevention or treatment of skin GVHD, a major medical problem in transplantation therapy of patients with malignancies. ..
- Ontogeny of Gut Dendritic Cells in Steady State and Inflamed SettingsMiriam Merad; Fiscal Year: 2007..Finally, our finding that induction of DC chimerism is critical to prevent GVHD leads us, in aim 3, to determine the nature of the circulating precursor that repopulates DCs in a clinically relevant model for allo-BMT. ..
- Dissecting the origin and the function of the cutaneous dendritic cell networkMiriam Merad; Fiscal Year: 2010..Program Director/Principal Investigator (Last, First, Middle): Merad, Miriam 1R01 AI080884-01A1 DISSECTING THE ORIGIN AND THE FUNCTION OF THE CUTANEOUS DENDRTITIC CELL NETWORK ..