Robert F Margolskee

Summary

Affiliation: Mount Sinai School of Medicine
Country: USA

Publications

  1. ncbi request reprint Molecular mechanisms of bitter and sweet taste transduction
    Robert F Margolskee
    Howard Hughes Medical Institute, Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 277:1-4. 2002
  2. pmc Immunocytochemical evidence for co-expression of Type III IP3 receptor with signaling components of bitter taste transduction
    T R Clapp
    Department of Anatomy and Neurobiology, Colorado State University, Fort Collins, CO 80523, USA
    BMC Neurosci 2:6. 2001
  3. pmc Mouse taste cells with G protein-coupled taste receptors lack voltage-gated calcium channels and SNAP-25
    Tod R Clapp
    Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA
    BMC Biol 4:7. 2006
  4. ncbi request reprint Umami taste responses are mediated by alpha-transducin and alpha-gustducin
    Wei He
    Department of Physiology and Biophysics, The Mount Sinai School of Medicine, New York, New York 10029, USA
    J Neurosci 24:7674-80. 2004
  5. ncbi request reprint Signal transduction of umami taste: insights from knockout mice
    Minqing Rong
    Department of Physiology and Biophysics, Howard Hughes Medical Institute, The Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    Chem Senses 30:i33-4. 2005
  6. ncbi request reprint Trpm5 null mice respond to bitter, sweet, and umami compounds
    Sami Damak
    Department of Neuroscience, The Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    Chem Senses 31:253-64. 2006
  7. ncbi request reprint Lactisole interacts with the transmembrane domains of human T1R3 to inhibit sweet taste
    Peihua Jiang
    Department of Neuroscience, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 280:15238-46. 2005
  8. ncbi request reprint The heterodimeric sweet taste receptor has multiple potential ligand binding sites
    Meng Cui
    Department of Physiology and Biophysics, Mount Sinai School of Medicine, Box 1218, New York, NY 10029, USA
    Curr Pharm Des 12:4591-600. 2006
  9. pmc T1R3 and gustducin in gut sense sugars to regulate expression of Na+-glucose cotransporter 1
    Robert F Margolskee
    Department of Neuroscience, Mount Sinai School of Medicine, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 104:15075-80. 2007
  10. ncbi request reprint Detection of sweet and umami taste in the absence of taste receptor T1r3
    Sami Damak
    Department of Biophysics and Physiology, Mount Sinai School of Medicine, Box 1677, 1425 Madison Avenue, New York, NY 10029, USA
    Science 301:850-3. 2003

Collaborators

Detail Information

Publications41

  1. ncbi request reprint Molecular mechanisms of bitter and sweet taste transduction
    Robert F Margolskee
    Howard Hughes Medical Institute, Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 277:1-4. 2002
  2. pmc Immunocytochemical evidence for co-expression of Type III IP3 receptor with signaling components of bitter taste transduction
    T R Clapp
    Department of Anatomy and Neurobiology, Colorado State University, Fort Collins, CO 80523, USA
    BMC Neurosci 2:6. 2001
    ..In the present study we used immunocytochemistry on rodent taste tissue to identify the IP3 receptors expressed in taste cells and to examine taste bud expression patterns for IP3R3...
  3. pmc Mouse taste cells with G protein-coupled taste receptors lack voltage-gated calcium channels and SNAP-25
    Tod R Clapp
    Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA
    BMC Biol 4:7. 2006
    ....
  4. ncbi request reprint Umami taste responses are mediated by alpha-transducin and alpha-gustducin
    Wei He
    Department of Physiology and Biophysics, The Mount Sinai School of Medicine, New York, New York 10029, USA
    J Neurosci 24:7674-80. 2004
    ..Umami detection involving alpha-gustducin and alpha(t-rod) occurs in anteriorly placed taste buds, however taste cells at the back of the tongue respond to umami compounds independently of these two G-protein subunits...
  5. ncbi request reprint Signal transduction of umami taste: insights from knockout mice
    Minqing Rong
    Department of Physiology and Biophysics, Howard Hughes Medical Institute, The Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    Chem Senses 30:i33-4. 2005
  6. ncbi request reprint Trpm5 null mice respond to bitter, sweet, and umami compounds
    Sami Damak
    Department of Neuroscience, The Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    Chem Senses 31:253-64. 2006
    ..Thus, while Trpm5 is an important factor in many taste responses, its absence does not eliminate all taste responses. We conclude that Trpm5-dependent and Trpm5-independent pathways underlie bitter, sweet, and umami tastes...
  7. ncbi request reprint Lactisole interacts with the transmembrane domains of human T1R3 to inhibit sweet taste
    Peihua Jiang
    Department of Neuroscience, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 280:15238-46. 2005
    ..In our model of T1R3's seven transmembrane helices, lactisole is predicted to dock to a binding pocket within the transmembrane region that includes these 4 key residues...
  8. ncbi request reprint The heterodimeric sweet taste receptor has multiple potential ligand binding sites
    Meng Cui
    Department of Physiology and Biophysics, Mount Sinai School of Medicine, Box 1218, New York, NY 10029, USA
    Curr Pharm Des 12:4591-600. 2006
    ....
  9. pmc T1R3 and gustducin in gut sense sugars to regulate expression of Na+-glucose cotransporter 1
    Robert F Margolskee
    Department of Neuroscience, Mount Sinai School of Medicine, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 104:15075-80. 2007
    ....
  10. ncbi request reprint Detection of sweet and umami taste in the absence of taste receptor T1r3
    Sami Damak
    Department of Biophysics and Physiology, Mount Sinai School of Medicine, Box 1677, 1425 Madison Avenue, New York, NY 10029, USA
    Science 301:850-3. 2003
    ..These results indicate that T1r3-independent sweet- and umami-responsive receptors and/or pathways exist in taste cells...
  11. ncbi request reprint Identification of the cyclamate interaction site within the transmembrane domain of the human sweet taste receptor subunit T1R3
    Peihua Jiang
    Department of Neuroscience and Physiology and Biophysics, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Biol Chem 280:34296-305. 2005
    ..The transmembrane domain of T1R3 is likely to play a critical role in the interconversion of the sweet receptor from the ground state to the active state...
  12. pmc Transsynaptic transport of wheat germ agglutinin expressed in a subset of type II taste cells of transgenic mice
    Sami Damak
    Department of Physiology and Biophysics, Mount Sinai School of Medicine, 1425 Madison Avenue, Box 1677, New York, New York, 10029, USA
    BMC Neurosci 9:96. 2008
    ..To determine how WGA produced in T1r3-expressing taste cells is transported into gustatory neurons, transgenic mice expressing WGA-IRES-GFP driven by the T1r3 promoter were generated...
  13. ncbi request reprint Ggamma13 interacts with PDZ domain-containing proteins
    Zairong Li
    Department of Neuroscience, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 281:11066-73. 2006
    ..The interaction of Ggamma13 with PDZ domain-containing proteins may provide a means to target particular Gbetagamma subunits to specific subcellular locations and/or macromolecular complexes involved in signaling pathways...
  14. ncbi request reprint Making sense with TRP channels: store-operated calcium entry and the ion channel Trpm5 in taste receptor cells
    Cristian A Perez
    Department of Physiology and Biophysics, Howard Hughes Medical Institute, Mount Sinai School of Medicine, New York University, New York, NY 10029, USA
    Cell Calcium 33:541-9. 2003
    ..The role of store-operated channels and Trpm5 in capacitative calcium entry in taste receptor cells in response to bitter compounds is discussed...
  15. pmc Umami responses in mouse taste cells indicate more than one receptor
    Yutaka Maruyama
    Department of Physiology and Biophysics, University of Miami Miller School of Medicine, Miami, Florida 33136, USA
    J Neurosci 26:2227-34. 2006
    ..Furthermore, umami responses were still observed in mutant mice lacking T1R3. A full explanation of umami taste transduction may involve novel combinations of the proposed receptors and/or as-yet-undiscovered taste receptors...
  16. pmc Taste signaling elements expressed in gut enteroendocrine cells regulate nutrient-responsive secretion of gut hormones
    Zaza Kokrashvili
    Department of Neuroscience, Mount Sinai School of Medicine, New York, NY 10029, USA
    Am J Clin Nutr 90:822S-825S. 2009
    ..Modulating hormone secretion from gut "taste cells" may provide novel treatments for obesity, diabetes, and malabsorption syndromes...
  17. ncbi request reprint The cysteine-rich region of T1R3 determines responses to intensely sweet proteins
    Peihua Jiang
    Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 279:45068-75. 2004
    ....
  18. pmc Phenoxy herbicides and fibrates potently inhibit the human chemosensory receptor subunit T1R3
    Emeline L Maillet
    Department of Neuroscience, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Med Chem 52:6931-5. 2009
    ..Likewise, phenoxy herbicides may have adverse metabolic effects in humans that would have gone undetected in studies on rodents...
  19. pmc Release of endogenous opioids from duodenal enteroendocrine cells requires Trpm5
    Zaza Kokrashvili
    Department of Neuroscience, Mount Sinai School of Medicine, New York, New York, USA
    Gastroenterology 137:598-606, 606.e1-2. 2009
    ..We studied a subset of these enteroendocrine cells in duodenum that produce several peptides, including endogenous opioids, and that also express the Trpm5 cation channel...
  20. pmc The role of T1r3 and Trpm5 in carbohydrate-induced obesity in mice
    John I Glendinning
    Department of Biology, Barnard College, Columbia University, 3009 Broadway, New York, NY 10027, USA
    Physiol Behav 107:50-8. 2012
    ..Our results suggest that nutritive solutions must be highly palatable to cause carbohydrate-induced obesity in mice, and that palatability produces this effect in part by enhancing nutrient utilization...
  21. ncbi request reprint Contribution of alpha-gustducin to taste-guided licking responses of mice
    John I Glendinning
    Department of Biological Science, Barnard College, Columbia University, 3009 Broadway, New York, NY 10027, USA
    Chem Senses 30:299-316. 2005
    ..There was no significant effect of deleting alpha-gustducin on licking responses to NH4Cl or HCl...
  22. doi request reprint T1r3 and alpha-gustducin in gut regulate secretion of glucagon-like peptide-1
    Zaza Kokrashvili
    Department of Neuroscience, Mount Sinai School of Medicine, New York, New York 10029, USA
    Ann N Y Acad Sci 1170:91-4. 2009
    ..Modulating hormone secretion from gut "taste cells" may provide novel treatments for obesity, diabetes, and malabsorption...
  23. ncbi request reprint Partial rescue of taste responses of alpha-gustducin null mice by transgenic expression of alpha-transducin
    Wei He
    Department of Physiology and Biophysics, Howard Hughes Medical Institute, Mount Sinai School of Medicine, 1425 Madison Avenue, Box 1677, New York, NY 10029, USA
    Chem Senses 27:719-27. 2002
    ..Our results also suggest that alpha-transducin and alpha-gustducin may differ, at least in part, in their function in these cells, although this conclusion must be qualified because of the limited fidelity of the transgene expression...
  24. ncbi request reprint G protein subunit G gamma 13 is coexpressed with G alpha o, G beta 3, and G beta 4 in retinal ON bipolar cells
    Liquan Huang
    Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Comp Neurol 455:1-10. 2003
    ..Gbeta1, 2, and 5 are expressed in partially overlapping subpopulations of cone bipolar cells. Ggamma13 and Gbeta3 and/or Gbeta4, thus, seem selectively to participate in signal transduction by ON bipolar cells...
  25. pmc Wnt signaling interacts with Shh to regulate taste papilla development
    Ken Iwatsuki
    Department of Neuroscience, Mount Sinai School of Medicine, 1425 Madison Avenue, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 104:2253-8. 2007
    ..Our observations indicate that Wnt/beta-catenin signaling and interactions between the Wnt and Shh pathways play essential roles in the development of fungiform papillae...
  26. ncbi request reprint A transient receptor potential channel expressed in taste receptor cells
    Cristian A Perez
    Howard Hughes Medical Institute, Mount Sinai School of Medicine, New York University, Box 1677, 1425 Madison Avenue, New York, New York 10029, USA
    Nat Neurosci 5:1169-76. 2002
    ..Trpm5 may be responsible for capacitative calcium entry in taste receptor cells that respond to bitter and/or sweet compounds...
  27. ncbi request reprint Teaching resources. Sensory systems: taste perception
    Robert F Margolskee
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, NY 10029, USA
    Sci STKE 2005:tr20. 2005
    ..quot; The lecture begins with a discussion of five distinct qualities of taste and then proceeds to describe receptors and signaling mechanisms...
  28. ncbi request reprint Taste receptor cell responses to the bitter stimulus denatonium involve Ca2+ influx via store-operated channels
    Tatsuya Ogura
    Department of Anatomy and Neurobiology, Colorado State University, Fort Collins, Colorado 80523, USA
    J Neurophysiol 87:3152-5. 2002
    ..These data suggest that SOCs may contribute to bitter taste transduction and to regulation of Ca2+ homeostasis in taste cells...
  29. pmc Taste cells of the gut and gastrointestinal chemosensation
    Josephine M Egan
    National Institute on Aging National Institutes of Health, Baltimore, MD 21224, USA
    Mol Interv 8:78-81. 2008
  30. ncbi request reprint Heat activation of TRPM5 underlies thermal sensitivity of sweet taste
    Karel Talavera
    Laboratorium voor Fysiologie, Campus Gasthuisberg, KU Leuven, B 3000 Leuven, Belgium
    Nature 438:1022-5. 2005
    ....
  31. pmc Gut-expressed gustducin and taste receptors regulate secretion of glucagon-like peptide-1
    Hyeung Jin Jang
    National Institute on Aging National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Proc Natl Acad Sci U S A 104:15069-74. 2007
    ..Modulating GLP-1 secretion in gut "taste cells" may provide an important treatment for obesity, diabetes and abnormal gut motility...
  32. ncbi request reprint Behavioral evidence for a role of alpha-gustducin in glutamate taste
    Collin J Ruiz
    Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA
    Chem Senses 28:573-9. 2003
    ..These data suggest that alpha-gustducin plays a role in glutamate taste...
  33. ncbi request reprint Role of the G-protein subunit alpha-gustducin in taste cell responses to bitter stimuli
    Alejandro Caicedo
    Department of Physiology and Biophysics and Program in Neuroscience, University of Miami School of Medicine, Miami, Florida 33136, USA
    J Neurosci 23:9947-52. 2003
    ..The reduced behavioral sensitivity to bitter stimuli in alpha-gustducin knock-out mice thus appears to be the consequence of a reduced number of bitter-activated taste cells, as well as reduced sensitivity...
  34. pmc Fat and carbohydrate preferences in mice: the contribution of alpha-gustducin and Trpm5 taste-signaling proteins
    Anthony Sclafani
    Dept of Psychology, Brooklyn College of the City University of New York, Brooklyn, NY 11210, USA
    Am J Physiol Regul Integr Comp Physiol 293:R1504-13. 2007
    ..The experience-induced rescue of fat and Polycose preferences in the KO mice likely reflects the action of a postoral-conditioning mechanism, which functions in the absence of alpha-gustducin and Trpm5...
  35. pmc Afferent neurotransmission mediated by hemichannels in mammalian taste cells
    Roman A Romanov
    Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia
    EMBO J 26:657-67. 2007
    ..Altogether, our data indicate that tastant-responsive taste cells release the neurotransmitter ATP via a non-exocytotic mechanism dependent upon the generation of an action potential...
  36. ncbi request reprint Taste responses to sweet stimuli in alpha-gustducin knockout and wild-type mice
    Vicktoria Danilova
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, WI, USA
    Chem Senses 31:573-80. 2006
    ..The differences between the responses in WT and KO mice were more pronounced in the CT than in the NG. These data indicate that alpha-gustducin participates in the transduction of the sweet taste in general...
  37. ncbi request reprint Assaying G protein-phosphodiesterase interactions in sensory systems
    Kosei Moriyama
    Section of Internal Medicine, Department of Medicine, Fukuoko Dental College, Sawara ku, Fukuoka 814 0193, Japan
    Methods Enzymol 345:37-48. 2002
  38. ncbi request reprint Immuno-localization of vesicular acetylcholine transporter in mouse taste cells and adjacent nerve fibers: indication of acetylcholine release
    Tatsuya Ogura
    Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, MD 21250, USA
    Cell Tissue Res 330:17-28. 2007
    ..Thus, functions of taste receptor cells could be modulated by trigeminal fibers via ACh release as well...
  39. ncbi request reprint TRPM5-expressing solitary chemosensory cells respond to odorous irritants
    Weihong Lin
    Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, MD 21250, USA
    J Neurophysiol 99:1451-60. 2008
    ..Together, our data show that the TRPM5-expressing cells respond to a variety of chemicals at high exposure levels typical of irritants and are positioned in the nasal cavity appropriately to monitor inhaled air quality...
  40. ncbi request reprint The taste transduction channel TRPM5 is a locus for bitter-sweet taste interactions
    Karel Talavera
    Laboratory of Ion Channel Research, Dept of Molecular Cell Biology, Herestraat 49, Campus Gasthuisberg, O and N1, KU Leuven, B 3000 Leuven, Belgium
    FASEB J 22:1343-55. 2008
    ..The inhibition of TRPM5 by bitter compounds constitutes the molecular basis of a novel mechanism of taste interactions, whereby the bitter tastant inhibits directly the sweet transduction pathway...
  41. ncbi request reprint Electrophysiological characterization of voltage-gated currents in defined taste cell types of mice
    Kathryn F Medler
    Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523, USA
    J Neurosci 23:2608-17. 2003
    ..Unexpectedly, all gustducin-expressing cells lacked voltage-gated Ca(2+) currents, suggesting that these cells use mechanisms other than classical synapses to communicate signals to the brain...