Research TopicsGenomes and Genes | Robert F MargolskeeSummaryAffiliation: Mount Sinai School of Medicine Country: USA Publications
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Publications
Immunocytochemical evidence for co-expression of Type III IP3 receptor with signaling components of bitter taste transductionT R Clapp
Department of Anatomy and Neurobiology, Colorado State University, Fort Collins, CO 80523, USA
BMC Neurosci 2:6. 2001..In the present study we used immunocytochemistry on rodent taste tissue to identify the IP3 receptors expressed in taste cells and to examine taste bud expression patterns for IP3R3...- Mouse taste cells with G protein-coupled taste receptors lack voltage-gated calcium channels and SNAP-25Tod R Clapp
Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA
BMC Biol 4:7. 2006.... 
Molecular mechanisms of bitter and sweet taste transductionRobert F Margolskee
Howard Hughes Medical Institute, Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York 10029, USA
J Biol Chem 277:1-4. 2002- Teaching resources. Sensory systems: taste perceptionRobert F Margolskee
Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, NY 10029, USA
Sci STKE 2005:tr20. 2005..quot; The lecture begins with a discussion of five distinct qualities of taste and then proceeds to describe receptors and signaling mechanisms... - Heat activation of TRPM5 underlies thermal sensitivity of sweet tasteKarel Talavera
Laboratorium voor Fysiologie, Campus Gasthuisberg, KU Leuven, B 3000 Leuven, Belgium
Nature 438:1022-5. 2005.... - Trpm5 null mice respond to bitter, sweet, and umami compoundsSami Damak
Department of Neuroscience, The Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
Chem Senses 31:253-64. 2006..Thus, while Trpm5 is an important factor in many taste responses, its absence does not eliminate all taste responses. We conclude that Trpm5-dependent and Trpm5-independent pathways underlie bitter, sweet, and umami tastes... - Umami responses in mouse taste cells indicate more than one receptorYutaka Maruyama
Department of Physiology and Biophysics, University of Miami Miller School of Medicine, Miami, Florida 33136, USA
J Neurosci 26:2227-34. 2006..Furthermore, umami responses were still observed in mutant mice lacking T1R3. A full explanation of umami taste transduction may involve novel combinations of the proposed receptors and/or as-yet-undiscovered taste receptors... - Taste responses to sweet stimuli in alpha-gustducin knockout and wild-type miceVicktoria Danilova
Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, WI, USA
Chem Senses 31:573-80. 2006..The differences between the responses in WT and KO mice were more pronounced in the CT than in the NG. These data indicate that alpha-gustducin participates in the transduction of the sweet taste in general... - The heterodimeric sweet taste receptor has multiple potential ligand binding sitesMeng Cui
Department of Physiology and Biophysics, Mount Sinai School of Medicine, Box 1218, New York, NY 10029, USA
Curr Pharm Des 12:4591-600. 2006.... - Afferent neurotransmission mediated by hemichannels in mammalian taste cellsRoman A Romanov
Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia
EMBO J 26:657-67. 2007..Altogether, our data indicate that tastant-responsive taste cells release the neurotransmitter ATP via a non-exocytotic mechanism dependent upon the generation of an action potential... - Wnt signaling interacts with Shh to regulate taste papilla developmentKen Iwatsuki
Department of Neuroscience, Mount Sinai School of Medicine, 1425 Madison Avenue, New York, NY 10029, USA
Proc Natl Acad Sci U S A 104:2253-8. 2007..Our observations indicate that Wnt/beta-catenin signaling and interactions between the Wnt and Shh pathways play essential roles in the development of fungiform papillae... - Fat and carbohydrate preferences in mice: the contribution of alpha-gustducin and Trpm5 taste-signaling proteinsAnthony Sclafani
Dept of Psychology, Brooklyn College of the City University of New York, Brooklyn, NY 11210, USA
Am J Physiol Regul Integr Comp Physiol 293:R1504-13. 2007..The experience-induced rescue of fat and Polycose preferences in the KO mice likely reflects the action of a postoral-conditioning mechanism, which functions in the absence of alpha-gustducin and Trpm5... - Gut-expressed gustducin and taste receptors regulate secretion of glucagon-like peptide-1Hyeung Jin Jang
National Institute on Aging National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
Proc Natl Acad Sci U S A 104:15069-74. 2007..Modulating GLP-1 secretion in gut "taste cells" may provide an important treatment for obesity, diabetes and abnormal gut motility... - T1R3 and gustducin in gut sense sugars to regulate expression of Na+-glucose cotransporter 1Robert F Margolskee
Department of Neuroscience, Mount Sinai School of Medicine, New York, NY 10029, USA
Proc Natl Acad Sci U S A 104:15075-80. 2007.... - Contribution of alpha-gustducin to taste-guided licking responses of miceJohn I Glendinning
Department of Biological Science, Barnard College, Columbia University, 3009 Broadway, New York, NY 10027, USA
Chem Senses 30:299-316. 2005..There was no significant effect of deleting alpha-gustducin on licking responses to NH4Cl or HCl... - Signal transduction of umami taste: insights from knockout miceMinqing Rong
Department of Physiology and Biophysics, Howard Hughes Medical Institute, The Mount Sinai School of Medicine, 1 Gustave L. Levy Place, New York, NY 10029, USA
Chem Senses 30:i33-4. 2005 - Taste cells of the gut and gastrointestinal chemosensationJosephine M Egan
National Institute on Aging/National Institutes of Health, Baltimore, MD 21224, USA
Mol Interv 8:78-81. 2008 - Partial rescue of taste responses of alpha-gustducin null mice by transgenic expression of alpha-transducinWei He
Department of Physiology and Biophysics, Howard Hughes Medical Institute, Mount Sinai School of Medicine, 1425 Madison Avenue, Box 1677, New York, NY 10029, USA
Chem Senses 27:719-27. 2002..Our results also suggest that alpha-transducin and alpha-gustducin may differ, at least in part, in their function in these cells, although this conclusion must be qualified because of the limited fidelity of the transgene expression... - Taste receptor cell responses to the bitter stimulus denatonium involve Ca2+ influx via store-operated channelsTatsuya Ogura
Department of Anatomy and Neurobiology, Colorado State University, Fort Collins, Colorado 80523, USA
J Neurophysiol 87:3152-5. 2002..These data suggest that SOCs may contribute to bitter taste transduction and to regulation of Ca2+ homeostasis in taste cells... - Detection of sweet and umami taste in the absence of taste receptor T1r3Sami Damak
Department of Biophysics and Physiology, Mount Sinai School of Medicine, Box 1677, 1425 Madison Avenue, New York, NY 10029, USA
Science 301:850-3. 2003..These results indicate that T1r3-independent sweet- and umami-responsive receptors and/or pathways exist in taste cells... - Behavioral evidence for a role of alpha-gustducin in glutamate tasteCollin J Ruiz
Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA
Chem Senses 28:573-9. 2003..These data suggest that alpha-gustducin plays a role in glutamate taste... - Role of the G-protein subunit alpha-gustducin in taste cell responses to bitter stimuliAlejandro Caicedo
Department of Physiology and Biophysics and Program in Neuroscience, University of Miami School of Medicine, Miami, Florida 33136, USA
J Neurosci 23:9947-52. 2003..The reduced behavioral sensitivity to bitter stimuli in alpha-gustducin knock-out mice thus appears to be the consequence of a reduced number of bitter-activated taste cells, as well as reduced sensitivity... - The cysteine-rich region of T1R3 determines responses to intensely sweet proteinsPeihua Jiang
Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York 10029, USA
J Biol Chem 279:45068-75. 2004.... - Umami taste responses are mediated by alpha-transducin and alpha-gustducinWei He
Department of Physiology and Biophysics, The Mount Sinai School of Medicine, New York, New York 10029, USA
J Neurosci 24:7674-80. 2004..Umami detection involving alpha-gustducin and alpha(t-rod) occurs in anteriorly placed taste buds, however taste cells at the back of the tongue respond to umami compounds independently of these two G-protein subunits... - A transient receptor potential channel expressed in taste receptor cellsCristian A Perez
Howard Hughes Medical Institute, Mount Sinai School of Medicine, New York University, Box 1677, 1425 Madison Avenue, New York, New York 10029, USA
Nat Neurosci 5:1169-76. 2002..Trpm5 may be responsible for capacitative calcium entry in taste receptor cells that respond to bitter and/or sweet compounds... - The taste transduction channel TRPM5 is a locus for bitter-sweet taste interactionsKarel Talavera
Laboratory of Ion Channel Research, Dept of Molecular Cell Biology, Herestraat 49, Campus Gasthuisberg, O and N1, KU Leuven, B 3000 Leuven, Belgium
FASEB J 22:1343-55. 2008..The inhibition of TRPM5 by bitter compounds constitutes the molecular basis of a novel mechanism of taste interactions, whereby the bitter tastant inhibits directly the sweet transduction pathway... - Assaying G protein-phosphodiesterase interactions in sensory systemsKosei Moriyama
Section of Internal Medicine, Department of Medicine, Fukuoko Dental College, Sawara-ku, Fukuoka 814-0193, Japan
Methods Enzymol 345:37-48. 2002 - TRPM5-expressing solitary chemosensory cells respond to odorous irritantsWeihong Lin
Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, MD 21250, USA
J Neurophysiol 99:1451-60. 2008..Together, our data show that the TRPM5-expressing cells respond to a variety of chemicals at high exposure levels typical of irritants and are positioned in the nasal cavity appropriately to monitor inhaled air quality... - Immuno-localization of vesicular acetylcholine transporter in mouse taste cells and adjacent nerve fibers: indication of acetylcholine releaseTatsuya Ogura
Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, MD 21250, USA
Cell Tissue Res 330:17-28. 2007..Thus, functions of taste receptor cells could be modulated by trigeminal fibers via ACh release as well... - G protein subunit G gamma 13 is coexpressed with G alpha o, G beta 3, and G beta 4 in retinal ON bipolar cellsLiquan Huang
Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York 10029, USA
J Comp Neurol 455:1-10. 2003..Gbeta1, 2, and 5 are expressed in partially overlapping subpopulations of cone bipolar cells. Ggamma13 and Gbeta3 and/or Gbeta4, thus, seem selectively to participate in signal transduction by ON bipolar cells... - Electrophysiological characterization of voltage-gated currents in defined taste cell types of miceKathryn F Medler
Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523, USA
J Neurosci 23:2608-17. 2003..Unexpectedly, all gustducin-expressing cells lacked voltage-gated Ca(2+) currents, suggesting that these cells use mechanisms other than classical synapses to communicate signals to the brain... - Making sense with TRP channels: store-operated calcium entry and the ion channel Trpm5 in taste receptor cellsCristian A Perez
Department of Physiology and Biophysics, Howard Hughes Medical Institute, Mount Sinai School of Medicine, New York University, New York, NY 10029, USA
Cell Calcium 33:541-9. 2003..The role of store-operated channels and Trpm5 in capacitative calcium entry in taste receptor cells in response to bitter compounds is discussed... - Ggamma13 interacts with PDZ domain-containing proteinsZairong Li
Department of Neuroscience, Mount Sinai School of Medicine, New York, New York 10029, USA
J Biol Chem 281:11066-73. 2006..The interaction of Ggamma13 with PDZ domain-containing proteins may provide a means to target particular Gbetagamma subunits to specific subcellular locations and/or macromolecular complexes involved in signaling pathways... - Identification of the cyclamate interaction site within the transmembrane domain of the human sweet taste receptor subunit T1R3Peihua Jiang
Department of Neuroscience and Physiology and Biophysics, Mount Sinai School of Medicine, New York, NY 10029, USA
J Biol Chem 280:34296-305. 2005..The transmembrane domain of T1R3 is likely to play a critical role in the interconversion of the sweet receptor from the ground state to the active state... - Lactisole interacts with the transmembrane domains of human T1R3 to inhibit sweet tastePeihua Jiang
Department of Neuroscience, Mount Sinai School of Medicine, New York, New York 10029, USA
J Biol Chem 280:15238-46. 2005..In our model of T1R3's seven transmembrane helices, lactisole is predicted to dock to a binding pocket within the transmembrane region that includes these 4 key residues...