TERRY KRULWICH

Summary

Affiliation: Mount Sinai School of Medicine
Country: USA

Publications

  1. pmc Molecular aspects of bacterial pH sensing and homeostasis
    Terry A Krulwich
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, New York 10029, USA
    Nat Rev Microbiol 9:330-43. 2011
  2. pmc A major role for social work input during development of an innovative Post-Baccalaureate Research Education Program in a medical center environment
    Terry Ann Krulwich
    Department of Pharmacology and Therapeutics, Mount Sinai School of Medicine, New York, New York 10029, USA
    Soc Work Health Care 48:653-64. 2009
  3. pmc Cation/proton antiporter complements of bacteria: why so large and diverse?
    Terry A Krulwich
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Mol Microbiol 74:257-60. 2009
  4. ncbi request reprint The Na(+)-dependence of alkaliphily in Bacillus
    T A Krulwich
    Department of Biochemistry and Molecular Biology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Biochim Biophys Acta 1505:158-68. 2001
  5. ncbi request reprint Functions of tetracycline efflux proteins that do not involve tetracycline
    T A Krulwich
    Department of Biochemistry and Molecular Biology, Mount Sinai School of Medicine, New York 10029, USA
    J Mol Microbiol Biotechnol 3:237-46. 2001
  6. pmc F1F0-ATP synthases of alkaliphilic bacteria: lessons from their adaptations
    David B Hicks
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Biochim Biophys Acta 1797:1362-77. 2010
  7. pmc A tenth atp gene and the conserved atpI gene of a Bacillus atp operon have a role in Mg2+ uptake
    David B Hicks
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 100:10213-8. 2003
  8. pmc The ATP synthase a-subunit of extreme alkaliphiles is a distinct variant: mutations in the critical alkaliphile-specific residue Lys-180 and other residues that support alkaliphile oxidative phosphorylation
    Makoto Fujisawa
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 285:32105-15. 2010
  9. pmc Catalytic properties of Staphylococcus aureus and Bacillus members of the secondary cation/proton antiporter-3 (Mrp) family are revealed by an optimized assay in an Escherichia coli host
    Talia H Swartz
    Department of Pharmacology and Biological Chemistry, Box 1603, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    J Bacteriol 189:3081-90. 2007
  10. pmc Characterization of the Functionally Critical AXAXAXA and PXXEXXP Motifs of the ATP Synthase c-Subunit from an Alkaliphilic Bacillus
    Jun Liu
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 284:8714-25. 2009

Collaborators

  • Jun Liu
  • David B Hicks
  • M Ito
  • Etana Padan
  • A Moir
  • G Sachs
  • Yanhe Ma
  • Horia Banciu
  • Yi Wei
  • Arthur A Guffanti
  • Jie Jin
  • Talia H Swartz
  • Magdia De Jesus
  • Makoto Fujisawa
  • Xiaoying Liu
  • David H Bechhofer
  • Markus Safferling
  • Oliver J Fackelmayer
  • Shinsuke Natsui
  • Xing Gong
  • Takayuki Ohira
  • Linda Yu
  • Chang An Yu
  • Benjamin Powers
  • Gintaras Deikus
  • Victor Shelden
  • Mark Nuqui
  • Da Neng Wang
  • Hilde Kloster
  • Thomas W Southworth
  • Josh Sharp
  • Caroline Ng
  • Heather Griffith
  • Sacha V Rood

Detail Information

Publications23

  1. pmc Molecular aspects of bacterial pH sensing and homeostasis
    Terry A Krulwich
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, New York 10029, USA
    Nat Rev Microbiol 9:330-43. 2011
    ..These insights may help us to target certain pathogens more accurately and to harness the capacities of environmental bacteria more efficiently...
  2. pmc A major role for social work input during development of an innovative Post-Baccalaureate Research Education Program in a medical center environment
    Terry Ann Krulwich
    Department of Pharmacology and Therapeutics, Mount Sinai School of Medicine, New York, New York 10029, USA
    Soc Work Health Care 48:653-64. 2009
    ..Social work and educational psychology principles inform strategies used to identify and address gaps or impediments that would otherwise diminish the potential of Mount Sinai PREP scholars to succeed in cutting-edge research careers...
  3. pmc Cation/proton antiporter complements of bacteria: why so large and diverse?
    Terry A Krulwich
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Mol Microbiol 74:257-60. 2009
    ..They also provide new perspectives on two large monovalent cation/proton antiporter families, the NhaC and the cation/proton antiporter-3 antiporter families...
  4. ncbi request reprint The Na(+)-dependence of alkaliphily in Bacillus
    T A Krulwich
    Department of Biochemistry and Molecular Biology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Biochim Biophys Acta 1505:158-68. 2001
    ..pseudofirmus OF4 requires a higher [Na(+)] for growth at near-neutral pH than at very alkaline pH values...
  5. ncbi request reprint Functions of tetracycline efflux proteins that do not involve tetracycline
    T A Krulwich
    Department of Biochemistry and Molecular Biology, Mount Sinai School of Medicine, New York 10029, USA
    J Mol Microbiol Biotechnol 3:237-46. 2001
    ..It will also be of interest to investigate the implications of Tet(L) and Tet(K) multifunctionality on the emergence and persistence of these antibiotic-resistance genes...
  6. pmc F1F0-ATP synthases of alkaliphilic bacteria: lessons from their adaptations
    David B Hicks
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Biochim Biophys Acta 1797:1362-77. 2010
    ..ATP synthesis in alkaliphiles depends upon alkaliphile-specific adaptations of the ATP synthase and there is also evidence for alkaliphile-specific adaptations of respiratory chain components...
  7. pmc A tenth atp gene and the conserved atpI gene of a Bacillus atp operon have a role in Mg2+ uptake
    David B Hicks
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 100:10213-8. 2003
    ..Such proteins could provide Mg2+, which is required by ATP synthase, and also support charge compensation, when the enzyme is functioning in the hydrolytic direction; e.g., during cytoplasmic pH regulation...
  8. pmc The ATP synthase a-subunit of extreme alkaliphiles is a distinct variant: mutations in the critical alkaliphile-specific residue Lys-180 and other residues that support alkaliphile oxidative phosphorylation
    Makoto Fujisawa
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 285:32105-15. 2010
    ..pseudofirmus OF4. Finally, a mutant B. pseudofirmus OF4 synthase with switched positions of Lys-180 (helix 4) and Gly-212 (helix 5) retained significant coupled synthase activity accompanied by proton leakiness...
  9. pmc Catalytic properties of Staphylococcus aureus and Bacillus members of the secondary cation/proton antiporter-3 (Mrp) family are revealed by an optimized assay in an Escherichia coli host
    Talia H Swartz
    Department of Pharmacology and Biological Chemistry, Box 1603, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    J Bacteriol 189:3081-90. 2007
    ..coli transformants led to a hypothesis explaining how activity of secondary, DeltaPsi-consuming antiporters can elicit increased capacity for DeltaPsi generation in a bacterial host...
  10. pmc Characterization of the Functionally Critical AXAXAXA and PXXEXXP Motifs of the ATP Synthase c-Subunit from an Alkaliphilic Bacillus
    Jun Liu
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 284:8714-25. 2009
    ..The results highlight the requirement for c-subunit adaptations to achieve alkaliphile ATP synthesis with minimal cytoplasmic proton loss and suggest partial suppression of some mutations by changes outside the atp operon...
  11. pmc Purification of two putative type II NADH dehydrogenases with different substrate specificities from alkaliphilic Bacillus pseudofirmus OF4
    Jun Liu
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    Biochim Biophys Acta 1777:453-61. 2008
    ..They were predominantly cytoplasmic in E. coli, consonant with the absence of antiport activity. The catalytic properties of NDH-2A were more consistent with a major respiratory role than those of NDH-2B...
  12. pmc Tet(L) and tet(K) tetracycline-divalent metal/H+ antiporters: characterization of multiple catalytic modes and a mutagenesis approach to differences in their efflux substrate and coupling ion preferences
    Jie Jin
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    J Bacteriol 184:4722-32. 2002
    ....
  13. pmc Three putative cation/proton antiporters from the soda lake alkaliphile Alkalimonas amylolytica N10 complement an alkali-sensitive Escherichia coli mutant
    Yi Wei
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Microbiology 153:2168-79. 2007
    ....
  14. ncbi request reprint Do physiological roles foster persistence of drug/multidrug-efflux transporters? A case study
    Terry A Krulwich
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, New York 10029, USA
    Nat Rev Microbiol 3:566-72. 2005
    ..The recent observations that Tet(L), a tetracycline-efflux transporter, and MdfA, a multidrug-efflux transporter, both confer alkali tolerance offer a striking case study in support of this hypothesis...
  15. pmc TetL tetracycline efflux protein from Bacillus subtilis is a dimer in the membrane and in detergent solution
    Markus Safferling
    Skirball Institute of Biomolecular Medicine and Department of Cell Biology, New York University School of Medicine, 540 First Avenue, New York, New York 10016, USA
    Biochemistry 42:13969-76. 2003
    ..In addition, TetL dimers were found in a number of other detergents and over a wide pH range. It is therefore likely that the oligomeric form of the protein in the membrane is also a dimer...
  16. pmc Mutational loss of a K+ and NH4+ transporter affects the growth and endospore formation of alkaliphilic Bacillus pseudofirmus OF4
    Yi Wei
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    J Bacteriol 185:5133-47. 2003
    ..Endospore formation in amino-acid-rich medium was significantly defective and germination was modestly defective in the orf9 and orf7-orf10 deletion mutants...
  17. pmc Adaptive gene expression in Bacillus subtilis strains deleted for tetL
    Yi Wei
    Department of Pharmacology and Biological Chemistry, Box 1603, Mount Sinai School of Medicine of New York University, New York, NY 10029, USA
    J Bacteriol 188:7090-100. 2006
    ....
  18. pmc Interaction between cytochrome caa3 and F1F0-ATP synthase of alkaliphilic Bacillus pseudofirmus OF4 is demonstrated by saturation transfer electron paramagnetic resonance and differential scanning calorimetry assays
    Xiaoying Liu
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    Biochemistry 46:306-13. 2007
    ..pseudofirmus OF4 in a reconstituted system. They support the suggestion that such an interaction between these complexes may contribute to sequestered proton transfers during alkaliphile oxidative phosphorylation at high pH...
  19. pmc Importance of the GP dipeptide of the antiporter motif and other membrane-embedded proline and glycine residues in tetracycline efflux protein Tet(L)
    Magdia De Jesus
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, New York 10029, USA
    Biochemistry 44:12896-904. 2005
    ..Patterns of Tc resistance conferred by additional Tet(L) mutants indicate important roles for another GP dipeptide in transmembrane segment (TMS) X as well as for membrane-embedded glycine residues in TMS XIII...
  20. ncbi request reprint An antiport mechanism for a member of the cation diffusion facilitator family: divalent cations efflux in exchange for K+ and H+
    Arthur A Guffanti
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, NY 10029, USA
    Mol Microbiol 45:145-53. 2002
    ..CzcO modestly enhances the activity of CzcD but is not predicted to be an integral membrane protein and has no antiport activity of its own...
  21. pmc Site-directed mutagenesis studies of selected motif and charged residues and of cysteines of the multifunctional tetracycline efflux protein Tet(L)
    Jie Jin
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Bacteriol 184:1796-800. 2002
    ..e., TET-Me(2+) and Na(+) efflux and K(+) uptake. Loop residues D74 and G70 are essential for TET flux but not for Na(+) or K(+) flux. A cysteineless Tet(L) protein exhibits all activities...
  22. pmc The Mrp Na+/H+ antiporter increases the activity of the malate:quinone oxidoreductase of an Escherichia coli respiratory mutant
    Talia H Swartz
    Box 1603, Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    J Bacteriol 187:388-91. 2005
    ....

Research Grants28

  1. Integrated Training in Pharmacological Sciences
    TERRY KRULWICH; Fiscal Year: 2007
    ....
  2. Post-Baccalaureate Research Education Program (PREP)
    TERRY KRULWICH; Fiscal Year: 2007
    ....
  3. Enhancing Successful Post-Baccalaureate Intervention to Promote Research Careers
    TERRY KRULWICH; Fiscal Year: 2007
    ..The studies test hypothesized factors as predictors/builders of successful PhD work and test the adaptability of successful PREP interventions to a doctoral program setting. ..
  4. Enhancing Successful Post-Baccalaureate Intervention to Promote Research Careers
    TERRY KRULWICH; Fiscal Year: 2009
    ..The studies test hypothesized factors as predictors/builders of successful PhD work and test the adaptability of successful PREP interventions to a doctoral program setting. ..
  5. Proton-Coupling Strategies in Alkaliphilic Bacillus
    Terry Ann Krulwich; Fiscal Year: 2010
    ....
  6. Enhancing Successful Post-Baccalaureate Intervention to Promote Research Careers
    Terry Ann Krulwich; Fiscal Year: 2010
    ..The studies test hypothesized factors as predictors/builders of successful PhD work and test the adaptability of successful PREP interventions to a doctoral program setting. ..
  7. Tet Efflux Proteins: Implications of Multiple Functions
    TERRY KRULWICH; Fiscal Year: 2004
    ..Specific Aim 3 will: clarify the basis for different phenotypes of tet(L) mutants; will further test the novel translational reinitiation model for tet(L) regulation; and will probe additional, Na- and pH related regulation. ..
  8. BACILLUS SUBTILIS TET(L)--REGULATION AND TRANSPORT
    TERRY KRULWICH; Fiscal Year: 1999
    ....
  9. PROTON COUPLING STRATEGIES IN ALKALIPHILIC BACILLUS
    TERRY KRULWICH; Fiscal Year: 2002
    ..MrpA may represent another trend that could emerge in energy-constrained contexts, i.e. the capacity for a secondary antiporter to adopt a primary type of energization in the proper protein complex or vice versa. ..
  10. Bacillus anthracis Spores: Initiation of Germination
    TERRY KRULWICH; Fiscal Year: 2003
    ..Such transporters may be new virulence factors, i.e. additional potential therapeutic targets. ..
  11. Proton-Coupling Strategies in Alkaliphilic Bacillus
    TERRY KRULWICH; Fiscal Year: 2009
    ..The studies of the Mrp system characterize a novel protein carrier found in many bacterial pathogens. Mrp is a potential target for development of new antibiotics. ..