Gordon M Keller

Summary

Affiliation: Mount Sinai School of Medicine
Country: USA

Publications

  1. ncbi request reprint Hematopoietic commitment of ES cells in culture
    Marion Kennedy
    Carl C Icahn Institute for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine, 1425 Madison Avenue, Box 1496, New York, New York 10029 6574, USA
    Methods Enzymol 365:39-59. 2003
  2. ncbi request reprint Embryonic stem cell differentiation: emergence of a new era in biology and medicine
    Gordon Keller
    Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
    Genes Dev 19:1129-55. 2005
  3. doi request reprint Human cardiovascular progenitor cells develop from a KDR+ embryonic-stem-cell-derived population
    Lei Yang
    Department of Gene and Cell Medicine, The Black Family Stem Cell Institute, Mount Sinai School of Medicine, 1425 Madison Avenue, New York, New York 10029, USA
    Nature 453:524-8. 2008
  4. pmc The homeobox gene HEX regulates proliferation and differentiation of hemangioblasts and endothelial cells during ES cell differentiation
    Atsushi Kubo
    Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    Blood 105:4590-7. 2005
  5. ncbi request reprint Multipotent flk-1+ cardiovascular progenitor cells give rise to the cardiomyocyte, endothelial, and vascular smooth muscle lineages
    Steven J Kattman
    Department of Gene and Cell Medicine, The Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York, New York 10029, USA
    Dev Cell 11:723-32. 2006
  6. pmc Numb mediates the interaction between Wnt and Notch to modulate primitive erythropoietic specification from the hemangioblast
    Xin Cheng
    Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    Development 135:3447-58. 2008
  7. pmc Developmental regulation of yolk sac hematopoiesis by Kruppel-like factor 6
    Nobuyuki Matsumoto
    Division of Liver Diseases, Department of Medicine, Mount Sinai School of Medicine, 1425 Madison Ave, Rm 1170F, New York, NY 10029, USA
    Blood 107:1357-65. 2006
  8. ncbi request reprint Committing embryonic stem cells to early endocrine pancreas in vitro
    Hsun Teresa Ku
    Department of Gene and Cell Medicine, Recanati Miller Transplantation Institute, Mount Sinai School of Medicine, New York, New York 10029 6574, USA
    Stem Cells 22:1205-17. 2004
  9. pmc An endothelial cell niche induces hepatic specification through dual repression of Wnt and Notch signaling
    Songyan Han
    Department of Gene and Cell Medicine, Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York, New York 10029, USA
    Stem Cells 29:217-28. 2011
  10. pmc Wnt and TGF-beta signaling are required for the induction of an in vitro model of primitive streak formation using embryonic stem cells
    Paul Gadue
    Department of Gene and Cell Medicine, Black Family Stem Cell Institute, Mount Sinai School of Medicine, 1 Gustave Levy Place, Box 1496, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 103:16806-11. 2006

Research Grants

  1. Hematopoietic Development From ES Cells
    Gordon Keller; Fiscal Year: 2007
  2. Endoderm Induction and Pancreatic Specification from ES*
    Gordon Keller; Fiscal Year: 2007

Collaborators

Detail Information

Publications36

  1. ncbi request reprint Hematopoietic commitment of ES cells in culture
    Marion Kennedy
    Carl C Icahn Institute for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine, 1425 Madison Avenue, Box 1496, New York, New York 10029 6574, USA
    Methods Enzymol 365:39-59. 2003
  2. ncbi request reprint Embryonic stem cell differentiation: emergence of a new era in biology and medicine
    Gordon Keller
    Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
    Genes Dev 19:1129-55. 2005
    ....
  3. doi request reprint Human cardiovascular progenitor cells develop from a KDR+ embryonic-stem-cell-derived population
    Lei Yang
    Department of Gene and Cell Medicine, The Black Family Stem Cell Institute, Mount Sinai School of Medicine, 1425 Madison Avenue, New York, New York 10029, USA
    Nature 453:524-8. 2008
    ..Together, these findings identify a human cardiovascular progenitor that defines one of the earliest stages of human cardiac development...
  4. pmc The homeobox gene HEX regulates proliferation and differentiation of hemangioblasts and endothelial cells during ES cell differentiation
    Atsushi Kubo
    Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    Blood 105:4590-7. 2005
    ....
  5. ncbi request reprint Multipotent flk-1+ cardiovascular progenitor cells give rise to the cardiomyocyte, endothelial, and vascular smooth muscle lineages
    Steven J Kattman
    Department of Gene and Cell Medicine, The Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York, New York 10029, USA
    Dev Cell 11:723-32. 2006
    ..Isolation and characterization of this previously unidentified population suggests that the mammalian cardiovascular system develops from multipotential progenitors...
  6. pmc Numb mediates the interaction between Wnt and Notch to modulate primitive erythropoietic specification from the hemangioblast
    Xin Cheng
    Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    Development 135:3447-58. 2008
    ..Together, these findings demonstrate that specification of the primitive erythroid lineage is controlled, in part, by the coordinated interaction of the Wnt and Notch pathways, and position Numb as a key mediator of this process...
  7. pmc Developmental regulation of yolk sac hematopoiesis by Kruppel-like factor 6
    Nobuyuki Matsumoto
    Division of Liver Diseases, Department of Medicine, Mount Sinai School of Medicine, 1425 Madison Ave, Rm 1170F, New York, NY 10029, USA
    Blood 107:1357-65. 2006
    ..Forced expression of KLF6 using a tet-inducible system enhanced the hematopoietic potential of wild-type EBs. Collectively, these findings implicate Klf6 in ES-cell differentiation and hematopoiesis...
  8. ncbi request reprint Committing embryonic stem cells to early endocrine pancreas in vitro
    Hsun Teresa Ku
    Department of Gene and Cell Medicine, Recanati Miller Transplantation Institute, Mount Sinai School of Medicine, New York, New York 10029 6574, USA
    Stem Cells 22:1205-17. 2004
    ..73% of the total population, compared with the control culture, which gave rise to less than 1% insulin-staining cells. These findings suggest that stepwise culture manipulations can direct ES cells to become early endocrine pancreas...
  9. pmc An endothelial cell niche induces hepatic specification through dual repression of Wnt and Notch signaling
    Songyan Han
    Department of Gene and Cell Medicine, Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York, New York 10029, USA
    Stem Cells 29:217-28. 2011
    ....
  10. pmc Wnt and TGF-beta signaling are required for the induction of an in vitro model of primitive streak formation using embryonic stem cells
    Paul Gadue
    Department of Gene and Cell Medicine, Black Family Stem Cell Institute, Mount Sinai School of Medicine, 1 Gustave Levy Place, Box 1496, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 103:16806-11. 2006
    ....
  11. pmc Sequential development of hematopoietic and cardiac mesoderm during embryonic stem cell differentiation
    Valerie Kouskoff
    Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 102:13170-5. 2005
    ..Access to these populations provides a unique approach to isolate and characterize the earliest progenitors of these lineages...
  12. doi request reprint Development and function of myeloid-derived suppressor cells generated from mouse embryonic and hematopoietic stem cells
    Zuping Zhou
    Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
    Stem Cells 28:620-32. 2010
    ..The successful in vitro generation of MDSCs may represent a critical step toward potential clinical application of MDSCs...
  13. ncbi request reprint Mouse embryonic stem cell-derived embryoid bodies generate progenitors that integrate long term into renal proximal tubules in vivo
    Cecile Vigneau
    Division of Nephrology, Department of Medicine, Mount Sinai School of Medicine, 1425 Madison Avenue, New York, NY 10029, USA
    J Am Soc Nephrol 18:1709-20. 2007
    ....
  14. ncbi request reprint Development of definitive endoderm from embryonic stem cells in culture
    Atsushi Kubo
    The Carl C Icahn Center for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    Development 131:1651-62. 2004
    ..These findings demonstrate that ES cells can generate endoderm in culture and, as such, establish this differentiation system as a unique murine model for studying the development and specification of this germ layer...
  15. pmc In vivo detection of embryonic stem cell-derived cardiovascular progenitor cells using Cy3-labeled Gadofluorine M in murine myocardium
    Eric D Adler
    Cardiovascular Institute and Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
    JACC Cardiovasc Imaging 2:1114-22. 2009
    ....
  16. ncbi request reprint Apoptosis in human glioblastoma cells produced using embryonic stem cell-derived astrocytes expressing tumor necrosis factor-related apoptosis-inducing ligand
    Isabelle M Germano
    Department of Neurosurgery, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Neurosurg 105:88-95. 2006
    ..The aim of this study was to test the proapoptotic effects of ES cell-derived astrocytes expressing transgenic tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in human malignant glioma cells...
  17. pmc Directed differentiation of mouse embryonic stem cells into thyroid follicular cells
    Maria C Arufe
    Division of Endocrinology, Diabetes, and Bone Diseases, Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
    Endocrinology 147:3007-15. 2006
    ..In addition, I- uptake activity was observed in these cells. Our results indicate that ES cells can be induced to differentiate into thyroid follicular cells, providing a powerful tool to study embryonic thyroid development and function...
  18. ncbi request reprint Runx1 is essential for hematopoietic commitment at the hemangioblast stage of development in vitro
    Georges Lacaud
    Carl C Icahn Institute for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine, New York, NY, USA
    Blood 100:458-66. 2002
    ....
  19. ncbi request reprint Embryonic stem cell-derived astrocytes: a novel gene therapy vector for brain tumors
    Mahmud Uzzaman
    Department of Neurosurgery, Mount Sinai School of Medicine, New York, New York 10029, USA
    Neurosurg Focus 19:E6. 2005
    ..The use of viral vectors is limited by several factors. The aim of this study was to assess whether embryonic stem cell (ESC)-derived astrocytes expressing a doxycyclineinducible transgene can be used as a vector for gene therapy...
  20. ncbi request reprint Embryonic stem cell-derived astrocytes expressing drug-inducible transgenes: differentiation and transplantion into the mouse brain
    Ronald J Benveniste
    Department of Neurosurgery, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Neurosurg 103:115-23. 2005
    ..The aim of this study was to generate highly pure populations of ESC-derived astrocytes expressing drug-inducible transgenes, while minimizing contamination by undifferentiated ESCs..
  21. ncbi request reprint Haemangioblast commitment is initiated in the primitive streak of the mouse embryo
    Tara L Huber
    Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York City, New York 10029, USA
    Nature 432:625-30. 2004
    ....
  22. ncbi request reprint Germ layer induction from embryonic stem cells
    Paul Gadue
    Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    Exp Hematol 33:955-64. 2005
    ..The current understanding of how these factors influence germ layer induction in both the embryo and in the ES cell differentiation system is addressed in this review...
  23. ncbi request reprint Committing embryonic stem cells to differentiate into thyrocyte-like cells in vitro
    Reigh Yi Lin
    Division of Endocrinology, Diabetes, and Bone Diseases, Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
    Endocrinology 144:2644-9. 2003
    ....
  24. ncbi request reprint Mouse Mix gene is activated early during differentiation of ES and F9 stem cells and induces endoderm in frog embryos
    Deanna Mohn
    Department of Medicine, Mount Sinai School of Medicine, New York, New York 10128, USA
    Dev Dyn 226:446-59. 2003
    ....
  25. doi request reprint Notch signaling respecifies the hemangioblast to a cardiac fate
    Vincent C Chen
    Department of Gene and Cell Medicine, The Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York, New York 10029, USA
    Nat Biotechnol 26:1169-78. 2008
    ..Together, these findings have uncovered a potential role for the Notch pathway in cardiac development and provide an approach for generating large numbers of cardiac progenitors from ES cells...
  26. pmc Acceleration of mesoderm development and expansion of hematopoietic progenitors in differentiating ES cells by the mouse Mix-like homeodomain transcription factor
    Stephen Willey
    Department of Medicine, Mt Sinai School of Medicine, New York, NY 10029 6574, USA
    Blood 107:3122-30. 2006
    ..Therefore, the mouse Mix gene functions early in the recruitment and/or expansion of mesodermal progenitors to the hemangioblastic and hematopoietic lineages...
  27. pmc Development of the hemangioblast defines the onset of hematopoiesis in human ES cell differentiation cultures
    Marion Kennedy
    Department of Gene and Cell Medicine, The Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York NY 10029, USA
    Blood 109:2679-87. 2007
    ..These findings demonstrate for the first time the existence of the human hemangioblast and in doing so identify the earliest stage of hematopoietic commitment...
  28. pmc SCL/Tal-1 is essential for hematopoietic commitment of the hemangioblast but not for its development
    Sunita L d'Souza
    Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    Blood 105:3862-70. 2005
    ....
  29. ncbi request reprint Enhanced proapoptotic effects of tumor necrosis factor-related apoptosis-inducing ligand on temozolomide-resistant glioma cells
    Mahmud Uzzaman
    Department of Neurosurgery, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Neurosurg 106:646-51. 2007
    ..The aim of this study was to assess whether TRAIL can be used as an adjuvant to temozolomide (TMZ) for apoptosis induction in malignant glioma cell lines...
  30. pmc In vivo gene delivery by embryonic-stem-cell-derived astrocytes for malignant gliomas
    Mahmud Uzzaman
    Department of neurosurgeruy, Mount Sinai School of Medicine, New York, NY 10029 USA
    Neuro Oncol 11:102-8. 2009
    ..Our data suggest that ESC-derived astrocytes conditionally expressing TRAIL should be considered as vectors to deliver gene therapy for malignant gliomas...
  31. ncbi request reprint Haploinsufficiency of Runx1 results in the acceleration of mesodermal development and hemangioblast specification upon in vitro differentiation of ES cells
    Georges Lacaud
    Carl C Icahn Center for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    Blood 103:886-9. 2004
    ..Our results indicate that Runx1 heterozygosity leads to an acceleration of mesodermal commitment and specification to the BL-CFCs and to the hematopoietic lineages in EBs...
  32. pmc The cardiomyocyte lineage is critical for optimization of stem cell therapy in a mouse model of myocardial infarction
    Eric D Adler
    Mount Sinai Hospital, New York, New York, USA
    FASEB J 24:1073-81. 2010
    ..Giovannone, S., Briley-Saebo, K., Young, W., Kattman, S., Mani, V., Laflamme, M., Zhu, W.-Z., Fayad, Z., Keller, G. The cardiomyocyte lineage is critical for optimization of stem cell therapy in a mouse model of myocardial infarction...
  33. ncbi request reprint BMP-4 is required for hepatic specification of mouse embryonic stem cell-derived definitive endoderm
    Valerie Gouon-Evans
    Department of Gene and Cell Medicine, Black Family Stem Cell Institute, Mount Sinai School of Medicine, 1425 Madison Avenue, New York, New York 10029, USA
    Nat Biotechnol 24:1402-11. 2006
    ..Together, these findings establish a developmental pathway in embryonic stem cell differentiation cultures that leads to efficient generation of cells with an immature hepatocytic phenotype...
  34. ncbi request reprint The in vitro differentiation of mouse embryonic stem cells into neutrophils
    Jonathan G Lieber
    Department of Medicine, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, Colorado 80206, USA
    Methods Enzymol 365:129-42. 2003
    ..As ES cells can be genetically modified, this system enables evaluation of the effects of specific genetic alterations on neutrophil differentiation and function...
  35. ncbi request reprint The in vitro production and characterization of neutrophils from embryonic stem cells
    Jonathan G Lieber
    National Jewish Medical and Research Center, 1400 Jackson St, Denver, CO 80206, USA
    Blood 103:852-9. 2004
    ..During the differentiation of these ES-derived neutrophils, regional areas of neutrophil production can be identified that have been designated as neutrophil generating regions (NGRs)...
  36. ncbi request reprint Specification of multipotential cardiovascular progenitor cells during embryonic stem cell differentiation and embryonic development
    Steven J Kattman
    McEwen Centre for Regenerative Medicine, University Health Network, Toronto, ON, Canada M5G 1L7
    Trends Cardiovasc Med 17:240-6. 2007
    ..These progenitor cells may also represent a novel cell population for models of congenital heart disease and cell replacement therapy...

Research Grants4

  1. Hematopoietic Development From ES Cells
    Gordon Keller; Fiscal Year: 2007
    ..Generation of LTRSC from human ES cells could ultimately expand the number of patients and the spectrum of diseases treated by hematopoietic stem cell transplantation. ..
  2. Endoderm Induction and Pancreatic Specification from ES*
    Gordon Keller; Fiscal Year: 2007
    ..The outcome of these experiments will provide new insights into the regulation of endoderm induction and pancreatic specification in the ES cell differentiation model ..