Adolfo Garcia-Sastre

Summary

Affiliation: Mount Sinai School of Medicine
Country: USA

Publications

  1. ncbi Novel vaccine strategies against emerging viruses
    Adolfo Garcia-Sastre
    Department of Microbiology, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, United States Global Health and Emerging Pathogens Institute, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, United States Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, United States Electronic address
    Curr Opin Virol 3:210-6. 2013
  2. ncbi Working safely with H5N1 viruses
    Adolfo Garcia-Sastre
    Department of Microbiology, Division of Infectious Diseases, Global Health and Emerging Pathogens Institute, Mount Sinai School of Medicine, New York, New York, USA
    MBio 3:e00049-12. 2012
  3. ncbi Induction and evasion of type I interferon responses by influenza viruses
    Adolfo Garcia-Sastre
    Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Virus Res 162:12-8. 2011
  4. ncbi 5'PPP-RNA induced RIG-I activation inhibits drug-resistant avian H5N1 as well as 1918 and 2009 pandemic influenza virus replication
    Priya Ranjan
    Influenza Division, NCIRD, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Virol J 7:102. 2010
  5. ncbi The immunological potency and therapeutic potential of a prototype dual vaccine against influenza and Alzheimer's disease
    Hayk Davtyan
    Department of Molecular Immunology, Institute for Molecular Medicine, Huntington Beach, CA 92647, USA
    J Transl Med 9:127. 2011
  6. ncbi Regulation of PURA gene transcription by three promoters generating distinctly spliced 5-prime leaders: a novel means of fine control over tissue specificity and viral signals
    Margaret J Wortman
    Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, 700 W, Olney Road, Norfolk, VA 23507, USA
    BMC Mol Biol 11:81. 2010
  7. ncbi Induction of type I interferon by RNA viruses: cellular receptors and their substrates
    Alina Baum
    Department of Microbiology, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    Amino Acids 38:1283-99. 2010
  8. ncbi Antiviral response in pandemic influenza viruses
    Adolfo Garcia-Sastre
    Mount Sinai School of Medicine, New York, New York 10028, USA
    Emerg Infect Dis 12:44-7. 2006
  9. ncbi Predicting the pathogenesis of influenza from genomic response: a step toward early diagnosis
    Eric Bortz
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    Genome Med 3:67. 2011
  10. ncbi Mechanisms of inhibition of the host interferon alpha/beta-mediated antiviral responses by viruses
    Adolfo Garcia-Sastre
    Department of Microbiology, Box 1124, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    Microbes Infect 4:647-55. 2002

Research Grants

  1. Virulence factors of influenza virus: The NS1 protein
    Adolfo Garcia Sastre; Fiscal Year: 2010
  2. TRANSFECTANT INFLUENZA VIRUSES IN CANCER THERAPY
    Adolfo Garcia Sastre; Fiscal Year: 2002
  3. Live attenuated vaccines for epidemic and pandemic flu
    Adolfo Garcia Sastre; Fiscal Year: 2007
  4. Virulence factors of influenza virus: The NS1 protein
    Adolfo Garcia Sastre; Fiscal Year: 2007
  5. VIRULENCE FACTORS OF INFLUENZA VIRUS--THE NS1 PROTEIN
    Adolfo Garcia Sastre; Fiscal Year: 2004

Detail Information

Publications93

  1. ncbi Novel vaccine strategies against emerging viruses
    Adolfo Garcia-Sastre
    Department of Microbiology, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, United States Global Health and Emerging Pathogens Institute, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, United States Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, United States Electronic address
    Curr Opin Virol 3:210-6. 2013
    ..These approaches might be applied to the control of many other emerging pathogens...
  2. ncbi Working safely with H5N1 viruses
    Adolfo Garcia-Sastre
    Department of Microbiology, Division of Infectious Diseases, Global Health and Emerging Pathogens Institute, Mount Sinai School of Medicine, New York, New York, USA
    MBio 3:e00049-12. 2012
    ..Rational countermeasures for preventing the spread of influenza can be developed only by gaining a thorough knowledge of the molecular mechanisms at work in host specificity and transmission...
  3. ncbi Induction and evasion of type I interferon responses by influenza viruses
    Adolfo Garcia-Sastre
    Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Virus Res 162:12-8. 2011
    ....
  4. ncbi 5'PPP-RNA induced RIG-I activation inhibits drug-resistant avian H5N1 as well as 1918 and 2009 pandemic influenza virus replication
    Priya Ranjan
    Influenza Division, NCIRD, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Virol J 7:102. 2010
    ..Activation of innate immune pathogen sensor Retinoic Acid Inducible Gene-I (RIG-I) has recently been shown to induce antiviral state...
  5. ncbi The immunological potency and therapeutic potential of a prototype dual vaccine against influenza and Alzheimer's disease
    Hayk Davtyan
    Department of Molecular Immunology, Institute for Molecular Medicine, Huntington Beach, CA 92647, USA
    J Transl Med 9:127. 2011
    ..We hypothesized that in elderly people with pre-existing memory Th cells specific to influenza this dual vaccine will simultaneously boost anti-influenza immunity and induce production of therapeutically active anti-Aβ antibodies...
  6. ncbi Regulation of PURA gene transcription by three promoters generating distinctly spliced 5-prime leaders: a novel means of fine control over tissue specificity and viral signals
    Margaret J Wortman
    Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, 700 W, Olney Road, Norfolk, VA 23507, USA
    BMC Mol Biol 11:81. 2010
    ..The distinct but related roles of Purα suggest a need for expression regulated differently depending on intracellular and external signals...
  7. ncbi Induction of type I interferon by RNA viruses: cellular receptors and their substrates
    Alina Baum
    Department of Microbiology, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    Amino Acids 38:1283-99. 2010
    ..We highlight the roles of TRL3, TLR7/8, retinoic acid inducible gene I, melanoma differentiation-associated gene 5, and the RNA molecules responsible for activating these viral sensors...
  8. ncbi Antiviral response in pandemic influenza viruses
    Adolfo Garcia-Sastre
    Mount Sinai School of Medicine, New York, New York 10028, USA
    Emerg Infect Dis 12:44-7. 2006
    ....
  9. ncbi Predicting the pathogenesis of influenza from genomic response: a step toward early diagnosis
    Eric Bortz
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    Genome Med 3:67. 2011
    ....
  10. ncbi Mechanisms of inhibition of the host interferon alpha/beta-mediated antiviral responses by viruses
    Adolfo Garcia-Sastre
    Department of Microbiology, Box 1124, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    Microbes Infect 4:647-55. 2002
    ..In this review, we will summarize recent research developments in this interesting field of virus-host cell interactions...
  11. ncbi Identification and characterization of viral antagonists of type I interferon in negative-strand RNA viruses
    A Garcia-Sastre
    Department of Microbiology, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    Curr Top Microbiol Immunol 283:249-80. 2004
    ..In many cases, these viral genes encode accessory pro- teins that are not required for viral infectivity but are critical for optimal replication and for virulence in the host...
  12. ncbi Influenza virus receptor specificity: disease and transmission
    Adolfo Garcia-Sastre
    Mount Sinai School of Medicine, Department of Microbiology, New York, NY 10029, USA
    Am J Pathol 176:1584-5. 2010
  13. ncbi Lessons learned from reconstructing the 1918 influenza pandemic
    Adolfo Garcia-Sastre
    Department of Microbiology, Mount Sinai School of Medicine, New York, NY, 10029, USA
    J Infect Dis 194:S127-32. 2006
    ..Monitoring the sequences of avian influenza viruses for genetic changes and diversity may help us to predict the risks that these viruses pose of causing a new pandemic...
  14. ncbi Inefficient control of host gene expression by the 2009 pandemic H1N1 influenza A virus NS1 protein
    Benjamin G Hale
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    J Virol 84:6909-22. 2010
    ..We discuss the impact that a natural future gain of this NS1 function may have on the new pandemic virus in humans...
  15. ncbi Species-specific inhibition of RIG-I ubiquitination and IFN induction by the influenza A virus NS1 protein
    Ricardo Rajsbaum
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York, United States of America
    PLoS Pathog 8:e1003059. 2012
    ..In conclusion, our results indicate that influenza NS1 protein targets TRIM25 and Riplet ubiquitin E3 ligases in a species-specific manner for the inhibition of RIG-I ubiquitination and antiviral IFN production...
  16. ncbi Negative role of RIG-I serine 8 phosphorylation in the regulation of interferon-beta production
    Estanislao Nistal-Villán
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, Box 1124, New York, NY 10029, USA
    J Biol Chem 285:20252-61. 2010
    ....
  17. ncbi Inhibition of interferon signaling by dengue virus
    Jorge L Munoz-Jordan
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave Levy Place, New York, NY 10029
    Proc Natl Acad Sci U S A 100:14333-8. 2003
    ..This protein, encoded by a positive strand RNA virus, is implicated as an IFN-signaling inhibitor...
  18. ncbi HERC6 is the main E3 ligase for global ISG15 conjugation in mouse cells
    Diede Oudshoorn
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York, United States of America
    PLoS ONE 7:e29870. 2012
    ..Together these data indicate that HerC6 is likely the functional counterpart of human HerC5 in mouse cells, suggesting that HerC6(-/-) mice may provide a feasible model to study the role of human HerC5 in antiviral responses...
  19. ncbi Global host immune response: pathogenesis and transcriptional profiling of type A influenza viruses expressing the hemagglutinin and neuraminidase genes from the 1918 pandemic virus
    John C Kash
    Department of Microbiology, School of Medicine, University of Washington, Seattle, Washington 98195 8070, USA
    J Virol 78:9499-511. 2004
    ..These studies document clear differences in gene expression profiles that were correlated with pulmonary disease pathology induced by virulent and attenuated influenza virus infections...
  20. ncbi Functional replacement of the carboxy-terminal two-thirds of the influenza A virus NS1 protein with short heterologous dimerization domains
    Xiuyan Wang
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    J Virol 76:12951-62. 2002
    ..These results suggest that the main biological function of the carboxy-terminal region of the NS1 protein of influenza A virus is the enhancement of its IFN antagonist properties by stabilizing the NS1 dimeric structure...
  21. ncbi Mutations in the NS1 C-terminal tail do not enhance replication or virulence of the 2009 pandemic H1N1 influenza A virus
    Benjamin G Hale
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    J Gen Virol 91:1737-42. 2010
    ..Our data suggest that the currently circulating 2009 H1N1 virus is optimized to replicate efficiently without requiring certain NS1 functions...
  22. ncbi Exponential enhancement of oncolytic vesicular stomatitis virus potency by vector-mediated suppression of inflammatory responses in vivo
    Jennifer Altomonte
    Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, New York, USA
    Mol Ther 16:146-53. 2008
    ....
  23. ncbi The influenza virus protein PB1-F2 inhibits the induction of type I interferon at the level of the MAVS adaptor protein
    Zsuzsanna T Varga
    Department of Microbiology, Mount Sinai School of Medicine, New York City, New York, United States of America
    PLoS Pathog 7:e1002067. 2011
    ..In summary, we have characterized the anti-interferon function of PB1-F2 and we suggest that this activity contributes to the enhanced pathogenicity seen with PB1-F2 N66S- expressing influenza viruses...
  24. ncbi Engineered RNA viral synthesis of microRNAs
    Andrew Varble
    Microbiology Graduate School Training Program, Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 107:11519-24. 2010
    ..This work demonstrates that RNA viruses can produce functional miRNAs and suggests that this level of transcriptional regulation may extend beyond DNA viruses...
  25. ncbi The Ebola virus VP35 protein inhibits activation of interferon regulatory factor 3
    Christopher F Basler
    Department of Microbiology, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    J Virol 77:7945-56. 2003
    ..These data suggest that in Ebola virus-infected cells, VP35 inhibits the induction of antiviral genes, including the IFN-beta gene, by blocking IRF-3 activation...
  26. ncbi The DBA.2 mouse is susceptible to disease following infection with a broad, but limited, range of influenza A and B viruses
    Natalie Pica
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, New York 10029, USA
    J Virol 85:12825-9. 2011
    ..Our data demonstrate the utility of DBA.2 mice for the elucidation of influenza virus pathogenicity determinants and the testing of influenza vaccines...
  27. ncbi PB1-F2 expression by the 2009 pandemic H1N1 influenza virus has minimal impact on virulence in animal models
    Rong Hai
    Department of Microbiology, Mount Sinai School of Medicine, 1 Gustave Levy Place, New York, NY 10029 6574, USA
    J Virol 84:4442-50. 2010
    ..In summary, our study demonstrates that PB1-F2 expression by the Cal/09 virus modulates the immune response to infection while having a minimal effect on virus virulence in two mammalian models...
  28. ncbi Contribution of double-stranded RNA and CPSF30 binding domains of influenza virus NS1 to the inhibition of type I interferon production and activation of human dendritic cells
    Irene Ramos
    Department of Microbiology, Division of Infectious Diseases, Mount Sinai School of Medicine, New York, New York, USA
    J Virol 87:2430-40. 2013
    ....
  29. ncbi Cross-presenting CD103+ dendritic cells are protected from influenza virus infection
    Julie Helft
    Department of Oncological Sciences, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Clin Invest 122:4037-47. 2012
    ..These results show that efficient cross-priming by migratory lung DCs is coupled to the acquisition of an anti-viral status, which is dependent on the type I IFN signaling pathway...
  30. ncbi Type I interferon induction pathway, but not released interferon, participates in the maturation of dendritic cells induced by negative-strand RNA viruses
    CAROLINA B LOPEZ
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    J Infect Dis 187:1126-36. 2003
    ..It was demonstrated that these pathways have different sensitivities to the presence of viral stimulus...
  31. ncbi Hematopoietic-specific targeting of influenza A virus reveals replication requirements for induction of antiviral immune responses
    Ryan A Langlois
    Department of Microbiology and Global Health and Emerging Pathogens Institute, Mount Sinai School of Medicine, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 109:12117-22. 2012
    ..Taking these data together, this research describes a unique platform to study the host response to IAV and provides insights into the mechanism of antigen presentation and the induction of IFN-I...
  32. ncbi Influenza B virus NS1-truncated mutants: live-attenuated vaccine approach
    Rong Hai
    Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Virol 82:10580-90. 2008
    ..We also provide evidence that influenza B virus NS1 mutants induce a self-adjuvanted immune response and confer effective protection against challenge with both homologous and heterologous B virus strains in mice...
  33. ncbi Blocking interhost transmission of influenza virus by vaccination in the guinea pig model
    Anice C Lowen
    Department of Microbiology, Mount Sinai School of Medicine, 1 Gustave Levy Pl, New York, NY 10029 6574, USA
    J Virol 83:2803-18. 2009
    ..In conclusion, we have shown that the guinea pig model can be used to test influenza virus vaccines and that the efficiency of transmission is a valuable readout when vaccine efficacy is evaluated...
  34. ncbi Analysis of in vivo dynamics of influenza virus infection in mice using a GFP reporter virus
    Balaji Manicassamy
    Department of Microbiology, and Global Health and Emerging Pathogens Institute, Mount Sinai School of Medicine, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 107:11531-6. 2010
    ....
  35. ncbi Inhibition of retinoic acid-inducible gene I-mediated induction of beta interferon by the NS1 protein of influenza A virus
    Masaki Mibayashi
    Department of Microbiology, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    J Virol 81:514-24. 2007
    ..Our results indicate that, in addition to sequestering dsRNA, the NS1 of influenza A virus binds to RIG-I and inhibits downstream activation of IRF-3, preventing the transcriptional induction of IFN-beta...
  36. ncbi Differential contribution of PB1-F2 to the virulence of highly pathogenic H5N1 influenza A virus in mammalian and avian species
    Mirco Schmolke
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York, United States of America
    PLoS Pathog 7:e1002186. 2011
    ..Our data could explain why PB1-F2 is conserved in avian influenza virus isolates and only impacts pathogenicity in mammals when containing certain amino acid motifs such as the rare N66S polymorphism...
  37. ncbi A combination in-ovo vaccine for avian influenza virus and Newcastle disease virus
    John Steel
    Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029 6574, USA
    Vaccine 26:522-31. 2008
    ..We propose that this virus has potential as a safe in-ovo live, attenuated, bivalent avian influenza and Newcastle disease virus vaccine...
  38. ncbi Attenuated influenza virus construct with enhanced hemagglutinin protein expression
    Jad Maamary
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York, USA
    J Virol 86:5782-90. 2012
    ....
  39. ncbi Hemagglutinin-pseudotyped green fluorescent protein-expressing influenza viruses for the detection of influenza virus neutralizing antibodies
    Luis Martinez-Sobrido
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    J Virol 84:2157-63. 2010
    ..These GFP-expressing influenza viruses replicate to high titers in HA-expressing cell lines, but in non-HA-expressing cells, their replication is restricted to a single cycle...
  40. ncbi The M segment of the 2009 new pandemic H1N1 influenza virus is critical for its high transmission efficiency in the guinea pig model
    Yi Ying Chou
    Department of Microbiology, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    J Virol 85:11235-41. 2011
    ....
  41. ncbi Strain-specific contribution of NS1-activated phosphoinositide 3-kinase signaling to influenza A virus replication and virulence
    Juan Ayllon
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York, USA
    J Virol 86:5366-70. 2012
    ..We hypothesize that specific spatially regulated NS1-activated PI3K signaling, rather than simply the total level of active PI3K, is important for virus replication and virulence...
  42. ncbi Recombinant influenza A viruses with enhanced levels of PB1 and PA viral protein expression
    Alan Belicha-Villanueva
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York, USA
    J Virol 86:5926-30. 2012
    ..Our findings highlight the possibility of increasing influenza virus protein expression and the need for a delicate balance between influenza viral replication, protein expression, and assembly...
  43. ncbi Oseltamivir-resistant variants of the 2009 pandemic H1N1 influenza A virus are not attenuated in the guinea pig and ferret transmission models
    Christopher W Seibert
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave Levy Place, New York, NY 10029 6574, USA
    J Virol 84:11219-26. 2010
    ..Our data suggest that the currently circulating pandemic 2009 H1N1 virus has a high potential to acquire drug resistance without losing fitness...
  44. ncbi The NS1 protein of influenza A virus suppresses interferon-regulated activation of antigen-presentation and immune-proteasome pathways
    Jennifer R Tisoncik
    Department of Microbiology, University of Washington, Seattle, WA 98195, USA
    J Gen Virol 92:2093-104. 2011
    ..This is the first report integrating genomic analysis from two independent human culture systems, including primary lung cells, using genetically similar H1N1 influenza viruses that differ only in the length of the NS1 protein...
  45. ncbi Vaccination with a synthetic peptide from the influenza virus hemagglutinin provides protection against distinct viral subtypes
    Taia T Wang
    Department of Microbiology and Medicine, Emerging Pathogens Institute, Mount Sinai School of Medicine, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 107:18979-84. 2010
    ..These results provide proof of concept for an HA2-based influenza vaccine that could diminish the threat of pandemic influenza disease and generally reduce the significance of influenza viruses as human pathogens...
  46. ncbi Protection of mice against lethal challenge with 2009 H1N1 influenza A virus by 1918-like and classical swine H1N1 based vaccines
    Balaji Manicassamy
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York, United States of America
    PLoS Pathog 6:e1000745. 2010
    ..Our results also support the notion that pigs can act as an animal reservoir where influenza virus HAs become antigenically frozen for long periods of time, facilitating the generation of human pandemic viruses...
  47. ncbi Genomic analysis of increased host immune and cell death responses induced by 1918 influenza virus
    John C Kash
    Department of Microbiology, University of Washington School of Medicine, Seattle, Washington 98195, USA
    Nature 443:578-81. 2006
    ..Moreover, understanding the contribution of host immune responses to virulent influenza virus infections is an important starting point for the identification of prognostic indicators and the development of novel antiviral therapies...
  48. ncbi The E3-ligase TRIM family of proteins regulates signaling pathways triggered by innate immune pattern-recognition receptors
    Gijs A Versteeg
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    Immunity 38:384-98. 2013
    ....
  49. ncbi Induction of protective immunity against malaria by priming-boosting immunization with recombinant cold-adapted influenza and modified vaccinia Ankara viruses expressing a CD8+-T-cell epitope derived from the circumsporozoite protein of Plasmodium yoelii
    Gloria Gonzalez-Aseguinolaza
    Department of Medical and Molecular Parasitology, NYU School of Medicine Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Virol 77:11859-66. 2003
    ..This vaccination regimen elicited high levels of protection against malaria. This is the first time that the vaccine efficacy of a recombinant cold-adapted influenza virus vector expressing a foreign antigen has been evaluated...
  50. ncbi Species-specific antagonism of host ISGylation by the influenza B virus NS1 protein
    Gijs A Versteeg
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    J Virol 84:5423-30. 2010
    ..Inability to block ISGylation in certain species may contribute to limiting influenza B virus host range...
  51. ncbi Pandemic 2009 H1N1 vaccine protects against 1918 Spanish influenza virus
    Rafael A Medina
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    Nat Commun 1:28. 2010
    ....
  52. ncbi Transcriptional role of p53 in interferon-mediated antiviral immunity
    Cesar Munoz-Fontela
    Department of Oncological Sciences, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Exp Med 205:1929-38. 2008
    ..Our results demonstrate that p53 contributes to innate immunity by enhancing IFN-dependent antiviral activity independent of its functions as a proapoptotic and tumor suppressor gene...
  53. ncbi Nipah virus V and W proteins have a common STAT1-binding domain yet inhibit STAT1 activation from the cytoplasmic and nuclear compartments, respectively
    Megan L Shaw
    Department of Microbiology, Box 1124, Mount Sinai School of Medicine, One Gustave L Levy Pl, New York, NY 10029, USA
    J Virol 78:5633-41. 2004
    ..We also show that the IFN antagonist activity of the P protein is not as strong as that of V or W, perhaps explaining why Nipah virus has evolved to express these two edited products...
  54. ncbi A reassortment-incompetent live attenuated influenza virus vaccine for protection against pandemic virus strains
    Rong Hai
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave Levy Place, New York, NY 10029 6574, USA
    J Virol 85:6832-43. 2011
    ..Overall, our data indicate that the chimeric live-attenuated influenza B viruses expressing the modified influenza A hemagglutinin are effective LAIVs...
  55. ncbi Identification of amino acid residues critical for the anti-interferon activity of the nucleoprotein of the prototypic arenavirus lymphocytic choriomeningitis virus
    Luis Martinez-Sobrido
    Department of Immunology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, New York 10029, USA
    J Virol 83:11330-40. 2009
    ..In addition, A549 cells infected with rLCMV/NP*(D382A), but not with WT LCMV, produced IFN and failed to rescue replication of the IFN-sensitive Newcastle disease virus...
  56. ncbi Transmission of pandemic H1N1 influenza virus and impact of prior exposure to seasonal strains or interferon treatment
    John Steel
    Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Virol 84:21-6. 2010
    ..In addition, the use of interferon as an antiviral prophylaxis may be an effective way to limit spread in at-risk populations...
  57. ncbi Inhibition of the alpha/beta interferon response by mouse hepatitis virus at multiple levels
    Jessica K Roth Cross
    Department of Microbiology, University of Pennsylvania, School of Medicine, 36th Street and Hamilton Walk, Philadelphia, PA 19104 6076, USA
    J Virol 81:7189-99. 2007
    ..These findings show that MHV employs unique strategies to circumvent the IFN-alpha/beta response at multiple steps...
  58. ncbi Contribution of NS1 effector domain dimerization to influenza A virus replication and virulence
    Juan Ayllon
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York, USA
    J Virol 86:13095-8. 2012
    ..These data suggest biological relevance for the W187-mediated homotypic interaction of NS1...
  59. ncbi Inhibition of alpha/beta interferon signaling by the NS4B protein of flaviviruses
    Jorge L Munoz-Jordan
    Department of Microbiology, Box 1124 Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, New York 10029, USA
    J Virol 79:8004-13. 2005
    ....
  60. ncbi A common polymorphism in the caspase recruitment domain of RIG-I modifies the innate immune response of human dendritic cells
    Jianzhong Hu
    Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Immunol 185:424-32. 2010
    ..Taken together, these data indicate that the innate immune response to viral infection of human cells is modified by a functional polymorphism in the RIG-I CARD...
  61. ncbi An enzymatic virus-like particle assay for sensitive detection of virus entry
    Donna M Tscherne
    Department of Microbiology, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    J Virol Methods 163:336-43. 2010
    ....
  62. ncbi Murine coronavirus delays expression of a subset of interferon-stimulated genes
    Kristine M Rose
    Department of Microbiology, University of Pennsylvania, School of Medicine, 36th Street and Hamilton Walk, Philadelphia, PA 19104 6076, USA
    J Virol 84:5656-69. 2010
    ..Transcription from an IRF-3-responsive promoter was partially inhibited by MHV; however, IRF-3 was transported to the nucleus and bound DNA in MHV-infected cells superinfected with SeV...
  63. ncbi Mouse STAT2 restricts early dengue virus replication
    Joseph Ashour
    Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Cell Host Microbe 8:410-21. 2010
    ..These results suggest that overcoming this restriction through transgenic mouse technology may help in the development of a long-sought immune-competent mouse model of dengue virus infection...
  64. ncbi Influenza A virus-generated small RNAs regulate the switch from transcription to replication
    Jasmine T Perez
    Microbiology Graduate School Training Program, Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 107:11525-30. 2010
    ..Taken together, the discovery of svRNA redefines the mechanistic switch of influenza virus transcription/replication and provides a potential target for broad-range, anti-influenza virus-based therapeutics...
  65. ncbi Dengue Virus Co-opts UBR4 to Degrade STAT2 and Antagonize Type I Interferon Signaling
    Juliet Morrison
    Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, United States of America Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, New York, United States of America
    PLoS Pathog 9:e1003265. 2013
    ..Our data underscore the importance of NS5-mediated STAT2 degradation in DENV replication and identify UBR4 as a host protein that is specifically exploited by DENV to inhibit IFN-I signaling via STAT2 degradation...
  66. ncbi Virulence determinants of pandemic influenza viruses
    Donna M Tscherne
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Clin Invest 121:6-13. 2011
    ..Identification and characterization of influenza A virus virulence determinants may provide insight into genotypic signatures of pathogenicity as well as a more thorough understanding of the factors that give rise to pandemics...
  67. ncbi Type 1 interferons and the virus-host relationship: a lesson in détente
    Adolfo Garcia-Sastre
    Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Science 312:879-82. 2006
    ..The plasticity that exists in these interactions provides us with a lesson in détente...
  68. ncbi p53 serves as a host antiviral factor that enhances innate and adaptive immune responses to influenza A virus
    Cesar Munoz-Fontela
    Department of Oncological Sciences, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Immunol 187:6428-36. 2011
    ..Thus, in addition to its established functions as a tumor suppressor gene, p53 serves as an IAV host antiviral factor that might be modulated to improve anti-IAV therapy and vaccines...
  69. ncbi rVSV(M Delta 51)-M3 is an effective and safe oncolytic virus for cancer therapy
    Lan Wu
    Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    Hum Gene Ther 19:635-47. 2008
    ....
  70. ncbi Ovarian tumor domain-containing viral proteases evade ubiquitin- and ISG15-dependent innate immune responses
    Natalia Frias-Staheli
    Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Cell Host Microbe 2:404-16. 2007
    ..Thus, the deconjugating activity of viral OTU proteases represents a unique viral strategy to inhibit Ub- and ISG15-dependent antiviral pathways...
  71. ncbi A novel paramyxovirus?
    Christopher F Basler
    Department of Microbiology, Mount Sinai School of Medicine, Box 1124, New York, NY 10029, USA
    Emerg Infect Dis 11:108-12. 2005
    ..These genes are sufficiently unique to suggest the existence of a novel paramyxovirus genus...
  72. ncbi Preference of RIG-I for short viral RNA molecules in infected cells revealed by next-generation sequencing
    Alina Baum
    Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 107:16303-8. 2010
    ..Our analysis for the first time identifies RIG-I PAMPs under natural infection conditions and implies that full-length genomes of single segmented RNA virus families are not bound by RIG-I during infection...
  73. ncbi The NS1 protein of a human influenza virus inhibits type I interferon production and the induction of antiviral responses in primary human dendritic and respiratory epithelial cells
    Kester Haye
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    J Virol 83:6849-62. 2009
    ..Lastly, C-terminal truncations in the NS1 protein of human influenza virus are sufficient to make the virus attenuated and more immunogenic, supporting its use as a live attenuated influenza vaccine in humans...
  74. ncbi Newcastle disease virus expressing a dendritic cell-targeted HIV gag protein induces a potent gag-specific immune response in mice
    Jad Maamary
    Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Virol 85:2235-46. 2011
    ..Here we demonstrate that the targeting of the HIV Gag antigen to DCs via the DEC205 receptor enhances the ability of an rNDV vector to induce a potent antigen-specific immune response...
  75. ncbi Transmission of influenza virus via aerosols and fomites in the guinea pig model
    Samira Mubareka
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Infect Dis 199:858-65. 2009
    ..The data provide new and much-needed insights into the modes of influenza virus spread and strain-specific differences in the efficiency of transmission...
  76. ncbi Recombinant Newcastle disease virus as a vaccine vector for cancer therapy
    ADAM VIGIL
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029, USA
    Mol Ther 16:1883-90. 2008
    ....
  77. ncbi Live attenuated influenza viruses containing NS1 truncations as vaccine candidates against H5N1 highly pathogenic avian influenza
    John Steel
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029 6574, USA
    J Virol 83:1742-53. 2009
    ....
  78. ncbi Development and characterization of a highly specific and sensitive SYBR green reverse transcriptase PCR assay for detection of the 2009 pandemic H1N1 influenza virus on the basis of sequence signatures
    Rafael A Medina
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Clin Microbiol 49:335-44. 2011
    ..Our approach highlights the use of systematic computational approaches to develop robust diagnostic tests during a viral pandemic...
  79. ncbi Differential recognition of viral RNA by RIG-I
    Alina Baum
    Department of Microbiology, Mount Sinai School of Medicine, New York, NY, USA
    Virulence 2:166-9. 2011
    ..In influenza virus infected cells RIG-I also preferentially associated with DI RNAs as well as with the shorter genomic segments...
  80. ncbi Macaque proteome response to highly pathogenic avian influenza and 1918 reassortant influenza virus infections
    Joseph N Brown
    Department of Microbiology, Washington National Primate Research Center, Seattle, WA 98195, USA
    J Virol 84:12058-68. 2010
    ..Proteomic results were integrated with previous genomic and pathological analysis to characterize the dynamic nature of the influenza virus infection process...
  81. ncbi Differential inhibition of type I interferon induction by arenavirus nucleoproteins
    Luis Martinez Sobrido
    Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Virol 81:12696-703. 2007
    ..Consistent with this observation, TCRV-infected cells also failed to inhibit interferon production...
  82. ncbi Hemagglutinin stalk antibodies elicited by the 2009 pandemic influenza virus as a mechanism for the extinction of seasonal H1N1 viruses
    Natalie Pica
    Department of Microbiology, Emerging Pathogens Institute, Mount Sinai School of Medicine, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 109:2573-8. 2012
    ..In addition, we describe assays that can be used to measure influenza virus-neutralizing antibodies that are not detected in the traditional hemagglutination inhibition assay...
  83. ncbi Structure of IRF-3 bound to the PRDIII-I regulatory element of the human interferon-beta enhancer
    Carlos R Escalante
    Department of Structural and Chemical Biology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Mol Cell 26:703-16. 2007
    ..Together, the structure and accompanying biological data provide insights into the assembly of the IFN-beta enhanceosome in mammals...
  84. ncbi Optimization of human immunodeficiency virus gag expression by newcastle disease virus vectors for the induction of potent immune responses
    Elena Carnero
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Virol 83:584-97. 2009
    ..These results indicate that strategies directed toward increasing antigen expression by NDV result in enhanced immunogenicity and vaccine efficacy...
  85. ncbi Viral tricks to grid-lock the type I interferon system
    Gijs A Versteeg
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    Curr Opin Microbiol 13:508-16. 2010
    ..Moreover, IFN antagonists are attractive targets for inhibition by small-molecule compounds...
  86. ncbi Detection of respiratory viruses and subtype identification of influenza A viruses by GreeneChipResp oligonucleotide microarray
    Phenix lan Quan
    Jerome L and Dawn Greene Infectious Disease Laboratory, Mailman School of Public Health, Columbia University, 722 West 168th Street, Room 1801, New York, NY 10032, USA
    J Clin Microbiol 45:2359-64. 2007
    ..Twenty-one different respiratory virus species were accurately characterized, including a recently identified novel genetic clade of rhinovirus...
  87. ncbi alpha-Defensin inhibits influenza virus replication by cell-mediated mechanism(s)
    Mirella Salvatore
    Division of Infectious Diseases, Mount Sinai School of Medicine, New York, NY 100129, USA
    J Infect Dis 196:835-43. 2007
    ..Our data expand the previously known activity of alpha -defensins against influenza virus. Characterizing the mechanism of action of alpha -defensins may lead to the identification of new strategies for prevention and therapy...
  88. ncbi Influenza A viruses: new research developments
    Rafael A Medina
    Department of Microbiology, and Global Health and Emerging Pathogens Institute, Mount Sinai School of Medicine
    Nat Rev Microbiol 9:590-603. 2011
    ..We also revise the lessons that have been learnt from the pandemic viruses of the past 100 years...
  89. ncbi The morphology and composition of influenza A virus particles are not affected by low levels of M1 and M2 proteins in infected cells
    Svetlana V Bourmakina
    Department of Microbiology, Mount Sinai School of Medicine, 1 Gustave L Levy Place, Box 1124, New York, NY 10029, USA
    J Virol 79:7926-32. 2005
    ....
  90. ncbi Enhancement of oncolytic properties of recombinant newcastle disease virus through antagonism of cellular innate immune responses
    Dmitriy Zamarin
    Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
    Mol Ther 17:697-706. 2009
    ..These findings demonstrate that modulation of innate immune responses by NDV results in enhancement of its oncolytic properties and warrant further investigation of this strategy in design of oncolytic NDV vectors against human tumors...
  91. ncbi Use of reverse genetics to enhance the oncolytic properties of Newcastle disease virus
    ADAM VIGIL
    Department of Microbiology, Medicine, Division of Infectious Diseases, Mount Sinai School of Medicine, New York, NY 10029, USA
    Cancer Res 67:8285-92. 2007
    ..Our data show the use of reverse genetics to develop enhanced recombinant NDV vectors as effective therapeutic agents for cancer treatment...
  92. ncbi NS5 of dengue virus mediates STAT2 binding and degradation
    Joseph Ashour
    Department of Microbiology, Emerging Pathogens Institute, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Virol 83:5408-18. 2009
    ..Therefore, we have identified both a novel mechanism by which DENV evades the innate immune response and a potential target for antiviral therapeutics...
  93. ncbi Evasion of innate and adaptive immune responses by influenza A virus
    Mirco Schmolke
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    Cell Microbiol 12:873-80. 2010
    ..We highlight well-characterized, as well as recently described features of this intriguing virus-host molecular battle...

Research Grants14

  1. Virulence factors of influenza virus: The NS1 protein
    Adolfo Garcia Sastre; Fiscal Year: 2010
    ..abstract_text> ..
  2. TRANSFECTANT INFLUENZA VIRUSES IN CANCER THERAPY
    Adolfo Garcia Sastre; Fiscal Year: 2002
    ..The proposed research may advance the field of cancer therapy towards possible novel ways to treat human tumors and prolong survival of cancer patients. ..
  3. Live attenuated vaccines for epidemic and pandemic flu
    Adolfo Garcia Sastre; Fiscal Year: 2007
    ..Finally, in collaboration with GreenHills Biotechnology, we will optimize cell culture techniques for the large-scale production of NS1-modified influenza virus vaccines suitable for use in humans. ..
  4. Virulence factors of influenza virus: The NS1 protein
    Adolfo Garcia Sastre; Fiscal Year: 2007
    ....
  5. VIRULENCE FACTORS OF INFLUENZA VIRUS--THE NS1 PROTEIN
    Adolfo Garcia Sastre; Fiscal Year: 2004
    ..These recombinant viruses will also be characterized in mice as potential live attenuated vaccines against influenza. ..