Research Topics
| CHRISTOPHER BASLERSummaryAffiliation: Mount Sinai School of Medicine Country: USA Publications
Research Grants
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Detail Information
Publications
Nipah and hendra virus interactions with the innate immune systemChristopher F Basler
Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
Curr Top Microbiol Immunol 359:123-52. 2012..With these systems in hand, the field is now poised to define how specific viral IFN-antagonist functions influence viral pathogenesis...
Progress in identifying virulence determinants of the 1918 H1N1 and the Southeast Asian H5N1 influenza A virusesChristopher F Basler
Department of Microbiology, Box 1124, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, United States
Antiviral Res 79:166-78. 2008..Defining how individual viral proteins promote enhanced replication, inflammation and severe disease will provide insight into the pathogenesis of severe influenza virus infections and suggest novel therapeutic approaches...- The Ebola virus VP35 protein inhibits activation of interferon regulatory factor 3Christopher F Basler
Department of Microbiology, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
J Virol 77:7945-56. 2003..These data suggest that in Ebola virus-infected cells, VP35 inhibits the induction of antiviral genes, including the IFN-beta gene, by blocking IRF-3 activation... - A novel paramyxovirus?Christopher F Basler
Department of Microbiology, Mount Sinai School of Medicine, Box 1124, New York, NY 10029, USA
Emerg Infect Dis 11:108-12. 2005..These genes are sufficiently unique to suggest the existence of a novel paramyxovirus genus... - Evasion of interferon responses by Ebola and Marburg virusesChristopher F Basler
Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029, USA
J Interferon Cytokine Res 29:511-20. 2009..Thus, despite their similarities, there are fundamental differences by which these deadly viruses counteract the IFN system. It will be of interest to determine how these differences influence pathogenesis... 
Influenza viruses: basic biology and potential drug targetsChristopher F Basler
Department of Microbiology, Box 1124, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
Infect Disord Drug Targets 7:282-93. 2007..However, the replication cycle of influenza viruses has been intensively studied and is receiving increased attention. New opportunities exist to develop novel antiviral strategies targeting these viruses...- Nipah virus V and W proteins have a common STAT1-binding domain yet inhibit STAT1 activation from the cytoplasmic and nuclear compartments, respectivelyMegan L Shaw
Department of Microbiology, Box 1124, Mount Sinai School of Medicine, One Gustave L Levy Pl, New York, NY 10029, USA
J Virol 78:5633-41. 2004..We also show that the IFN antagonist activity of the P protein is not as strong as that of V or W, perhaps explaining why Nipah virus has evolved to express these two edited products... - Mutations abrogating VP35 interaction with double-stranded RNA render Ebola virus avirulent in guinea pigsKathleen C Prins
Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029, USA
J Virol 84:3004-15. 2010..Moreover, these studies provide a framework for the development of antivirals targeting this critical EBOV virulence factor... - The tumour suppressor CYLD is a negative regulator of RIG-I-mediated antiviral responseConstantin S Friedman
Immunology Institute, Mount Sinai School of Medicine, Box 1630, One Gustave L Levy Place, New York, New York 10029, USA
EMBO Rep 9:930-6. 2008..These findings show that CYLD is a negative regulator of RIG-I-mediated innate antiviral response... - Newcastle disease virus V protein is a determinant of host range restrictionMan-Seong Park
Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029, USA
J Virol 77:9522-32. 2003..Taken together, these data suggest that the host range of NDV is limited by the ability of its V protein to efficiently prevent innate host defenses, such as the IFN response and apoptosis... - Ebola virus VP35 protein binds double-stranded RNA and inhibits alpha/beta interferon production induced by RIG-I signalingWashington B Cardenas
Department of Microbiology, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
J Virol 80:5168-78. 2006..These data support the hypothesis that dsRNA binding may contribute to VP35 IFN antagonist function. However, additional mechanisms of inhibition, at a point proximal to the IRF-3 kinases, most likely also exist... - Nuclear localization of the Nipah virus W protein allows for inhibition of both virus- and toll-like receptor 3-triggered signaling pathwaysMegan L Shaw
Department of Microbiology, Box 1124, Mount Sinai School of Medicine, One Gustave L Levy Pl, New York, NY 10029, USA
J Virol 79:6078-88. 2005.... - A five-amino-acid deletion of the eastern equine encephalitis virus capsid protein attenuates replication in mammalian systems but not in mosquito cellsPatricia V Aguilar
Department of Microbiology, Box 1124, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
J Virol 82:6972-83. 2008..This region is also critical for EEEV pathogenesis... - Effects of influenza A virus NS1 protein on protein expression: the NS1 protein enhances translation and is not required for shutoff of host protein synthesisMirella Salvatore
Department of Microbiology. Immunobiology Center, Mount Sinai School of Medicine, New York, New York 10029, USA
J Virol 76:1206-12. 2002..In contrast to the earlier proposal suggesting that the NS1 protein regulates the levels of spliced M2 mRNA, no effects on M2 protein accumulation were seen in Vero cells infected with delNS1 virus... - A recombinant influenza A virus expressing an RNA-binding-defective NS1 protein induces high levels of beta interferon and is attenuated in miceNicola R Donelan
Department of Microbiology. Microbiology Graduate School Training Program, Mount Sinai School of Medicine, New York, New York 10029, USA
J Virol 77:13257-66. 2003..These results suggest not only that the IFN antagonist properties of the NS1 protein depend on its ability to bind dsRNA but also that they can be modulated by amino acid residues not involved in RNA binding... - Neutralizing anti-influenza virus monoclonal antibodies: therapeutics and tools for discoveryOsvaldo Martinez
Department of Microbiology, Mount Sinai School of Medicine, New York, New York, USA
Int Rev Immunol 28:69-92. 2009.... - Homo-oligomerization facilitates the interferon-antagonist activity of the ebolavirus VP35 proteinSt Patrick Reid
Department of Microbiology, Box 1124, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
Virology 341:179-89. 2005.... - Nipah virus sequesters inactive STAT1 in the nucleus via a P gene-encoded mechanismMichael J Ciancanelli
Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
J Virol 83:7828-41. 2009..These data demonstrate that the gene for NiV P encodes functions that sequester inactive STAT1 in the nucleus, preventing its activation and suggest that the W protein is the dominant inhibitor of STAT1 in NiV-infected cells... - Marburg virus evades interferon responses by a mechanism distinct from ebola virusCharalampos Valmas
Department of Microbiology, Mount Sinai School of Medicine, New York, New York, USA
PLoS Pathog 6:e1000721. 2010..It identifies a novel function for the MARV VP40 protein and suggests that MARV may globally inhibit Jak1-dependent cytokine signaling... - Newcastle disease virus (NDV)-based assay demonstrates interferon-antagonist activity for the NDV V protein and the Nipah virus V, W, and C proteinsMan-Seong Park
Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029, USA
J Virol 77:1501-11. 2003.... - Capsid protein of eastern equine encephalitis virus inhibits host cell gene expressionPatricia V Aguilar
Department of Microbiology, Box 1124, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
J Virol 81:3866-76. 2007.... - Hemagglutinin-pseudotyped green fluorescent protein-expressing influenza viruses for the detection of influenza virus neutralizing antibodiesLuis Martinez-Sobrido
Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
J Virol 84:2157-63. 2010..These GFP-expressing influenza viruses replicate to high titers in HA-expressing cell lines, but in non-HA-expressing cells, their replication is restricted to a single cycle... - Ebola virus protein VP35 impairs the function of interferon regulatory factor-activating kinases IKKepsilon and TBK-1Kathleen C Prins
Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029, USA
J Virol 83:3069-77. 2009.... - Ebola virus VP24 binds karyopherin alpha1 and blocks STAT1 nuclear accumulationSt Patrick Reid
Department of Microbiology, Box 1124, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
J Virol 80:5156-67. 2006..These data suggest that VP24 is likely to be an important virulence determinant that allows EBOV to evade the antiviral effects of IFNs... - Functional replacement of the carboxy-terminal two-thirds of the influenza A virus NS1 protein with short heterologous dimerization domainsXiuyan Wang
Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
J Virol 76:12951-62. 2002..These results suggest that the main biological function of the carboxy-terminal region of the NS1 protein of influenza A virus is the enhancement of its IFN antagonist properties by stabilizing the NS1 dimeric structure... - The N- and C-terminal domains of the NS1 protein of influenza B virus can independently inhibit IRF-3 and beta interferon promoter activationNicola R Donelan
Department of Microbiology, Box 1124, Mount Sinai School of Medicine, 1 Gustave L. Levy Pl, New York, NY 10029, USA
J Virol 78:11574-82. 2004..Our results support the hypothesis that the NS1 protein of influenza B virus plays an important role in antagonizing the IRF-3- and IFN-induced antiviral host responses to virus infection... - Protection of mice against lethal challenge with 2009 H1N1 influenza A virus by 1918-like and classical swine H1N1 based vaccinesBalaji Manicassamy
Department of Microbiology, Mount Sinai School of Medicine, New York, New York, United States of America
PLoS Pathog 6:e1000745. 2010..Our results also support the notion that pigs can act as an animal reservoir where influenza virus HAs become antigenically frozen for long periods of time, facilitating the generation of human pandemic viruses... - Ebola virus-like particle-induced activation of NF-kappaB and Erk signaling in human dendritic cells requires the glycoprotein mucin domainOsvaldo Martinez
Department of Microbiology, Box 1124, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
Virology 364:342-54. 2007..We conclude that the GP mucin domain is required for VLPs to stimulate human dendritic cells through NF-kappaB and MAPK signaling pathways... - Ebola virus VP24 proteins inhibit the interaction of NPI-1 subfamily karyopherin alpha proteins with activated STAT1St Patrick Reid
Department of Microbiology, Box 1124, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
J Virol 81:13469-77. 2007.... - Nipah virus edits its P gene at high frequency to express the V and W proteinsSachin Kulkarni
Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
J Virol 83:3982-7. 2009..Eighty-two percent of transcripts are edited, with up to 11 G insertions observed. This exceptionally high editing frequency ensures expression of the V and W proteins... - A single amino acid substitution in 1918 influenza virus hemagglutinin changes receptor binding specificityLaurel Glaser
Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
J Virol 79:11533-6. 2005..Mutation of this single amino acid back to the avian consensus resulted in a preference for the avian receptor... - Zaire Ebola virus entry into human dendritic cells is insensitive to cathepsin L inhibitionOsvaldo Martinez
Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
Cell Microbiol 12:148-57. 2010..Thus, cathepsin L plays a minimal, if any, role in EBOV infection in human DCs. The inflammatory cytokine TNFalpha modulates cathepsin B activity and affects EBOV entry into and infection of human DCs... - Cellular transcriptional profiling in influenza A virus-infected lung epithelial cells: the role of the nonstructural NS1 protein in the evasion of the host innate defense and its potential contribution to pandemic influenzaGary K Geiss
Department of Microbiology, School of Medicine, University of Washington, Seattle, WA 98195, USA
Proc Natl Acad Sci U S A 99:10736-41. 2002.... - Global host immune response: pathogenesis and transcriptional profiling of type A influenza viruses expressing the hemagglutinin and neuraminidase genes from the 1918 pandemic virusJohn C Kash
Department of Microbiology, School of Medicine, University of Washington, Seattle, Washington 98195 8070, USA
J Virol 78:9499-511. 2004..These studies document clear differences in gene expression profiles that were correlated with pulmonary disease pathology induced by virulent and attenuated influenza virus infections... - Genomic analysis of increased host immune and cell death responses induced by 1918 influenza virusJohn C Kash
Department of Microbiology, University of Washington School of Medicine, Seattle, Washington 98195, USA
Nature 443:578-81. 2006..Moreover, understanding the contribution of host immune responses to virulent influenza virus infections is an important starting point for the identification of prognostic indicators and the development of novel antiviral therapies... - Mutation of YMYL in the Nipah virus matrix protein abrogates budding and alters subcellular localizationMichael J Ciancanelli
Department of Microbiology, Box 1124, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
J Virol 80:12070-8. 2006..When YMYL was fused to a late-domain mutant of the Ebola virus VP40 matrix protein, VP40 budding was restored. These results suggest that the YMYL sequence may act as a trafficking signal and a late domain for NiV M... - Marburg virus VP40 antagonizes interferon signaling in a species-specific mannerCharalampos Valmas
Department Microbiology, Box 1124, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
J Virol 85:4309-17. 2011..These data identify an apparent determinant of RAVV host range and virulence and define specific genetic determinants of this function... - Viruses and the type I interferon antiviral system: induction and evasionChristopher F Basler
Mount Sinai School of Medicine, Department of Microbiology, New York, NY, USA
Int Rev Immunol 21:305-37. 2002..Understanding the interplay between viral-encoded IFN antagonists and the interferon response will be essential if the therapeutic potential of IFNs is to be fully exploited... - Chimeric influenza A viruses with a functional influenza B virus neuraminidase or hemagglutininAstrid Flandorfer
Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029, USA
J Virol 77:9116-23. 2003..Taken together, we show here that the absence of reassortment between influenza viruses belonging to different A and B types is not due to spike glycoprotein incompatibility at the level of the full-length NA or of the HA ectodomain... - Structure of the uncleaved human H1 hemagglutinin from the extinct 1918 influenza virusJames Stevens
Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Science 303:1866-70. 2004.... - Single gene reassortants identify a critical role for PB1, HA, and NA in the high virulence of the 1918 pandemic influenza virusClaudia Pappas
Influenza Division, Mailstop G 16, National Center for Immunization and Respiratory Diseases, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road Northeast, Atlanta, GA 30333, USA
Proc Natl Acad Sci U S A 105:3064-9. 2008..These results highlight the importance of the 1918 HA, NA, and PB1 genes for optimal virus replication and virulence of this pandemic strain... - Characterization of the reconstructed 1918 Spanish influenza pandemic virusTerrence M Tumpey
Influenza Branch, Mailstop G 16, Division of Viral and Rickettsial Diseases DVRD, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, NE, Atlanta, GA 30333, USA
Science 310:77-80. 2005..Moreover, the coordinated expression of the 1918 virus genes most certainly confers the unique high-virulence phenotype observed with this pandemic virus... - Existing antivirals are effective against influenza viruses with genes from the 1918 pandemic virusTerrence M Tumpey
Southeast Poultry Research Laboratory, United States Department of Agriculture, Athens, GA 30605, USA
Proc Natl Acad Sci U S A 99:13849-54. 2002..These data suggest that current antiviral strategies would be effective in curbing the dangers of a re-emergent 1918 or 1918-like virus... - The makings of a killerPeter Palese
Nat Med 8:927-8. 2002 - Pathogenicity and immunogenicity of influenza viruses with genes from the 1918 pandemic virusTerrence M Tumpey
Southeast Poultry Research Laboratory, Agricultural Research Service, U S Department of Agriculture, Athens, GA 30605, USA
Proc Natl Acad Sci U S A 101:3166-71. 2004..These data suggest a strategy of vaccination that would be effective against a reemergent 1918 or 1918-like virus... - Sensing RNA virus infectionsChristopher F Basler
Nat Chem Biol 3:20-1. 2007 - Pathogenicity of influenza viruses with genes from the 1918 pandemic virus: functional roles of alveolar macrophages and neutrophils in limiting virus replication and mortality in miceTerrence M Tumpey
Influenza Branch, Mail Stop G 16, DVRD, NCID, Centers for Disease Control and Prevention, 1600 Clifton Road, N E, Atlanta, GA 30333, USA
J Virol 79:14933-44. 2005.... - Neutralizing antibodies derived from the B cells of 1918 influenza pandemic survivorsXiaocong Yu
Departments of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
Nature 455:532-6. 2008....
Research Grants
- EBOLA VIRUS INTERFERON-ANTAGONISTS AND VIRULENCECHRISTOPHER BASLER; Fiscal Year: 2003..VP35 and VP24 may also prove to be attractive targets for new antiviral agents. Inhibition of anti-IFN factors may sensitize Ebola virus infected cells to the antiviral effects of IFN and may also enhance the effect of antiviral agents. ..
- Lassa and Nipah Virus Interferon-AntagonistsCHRISTOPHER BASLER; Fiscal Year: 2003..abstract_text> ..
- Ebola VP35 Interferon-Antagonist: Mechanism and SignifCHRISTOPHER BASLER; Fiscal Year: 2009..abstract_text> ..
- Ebola virus VP24_a novel inhibitor of interferon and oxidative stress signalingChristopher F Basler; Fiscal Year: 2011..The experiments in this proposal will clarify the role of two viral proteins, VP24 and VP35, in EBOV disease and suggest new ways to prevent or treat these infections. ..