Research Topics
| David M WardSummaryAffiliation: Montana State University Country: USA Publications
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Detail Information
Publications
A natural species concept for prokaryotesD M Ward
Department of Microbiology, Montana State University, Bozeman MT, 59717, USA
Curr Opin Microbiol 1:271-7. 1998..The species debate can be exploited to address a larger issue - microbiologists need, in general, to take a more natural view of the organisms they study...
Genomics, environmental genomics and the issue of microbial speciesD M Ward
Department of Land Resources and Environmental Science, Montana State University, Bozeman, MT 59715, USA
Heredity (Edinb) 100:207-19. 2008....
Microbial diversity in natural environments: focusing on fundamental questionsDavid M Ward
Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, MT 59715 3120, USA
Antonie Van Leeuwenhoek 90:309-24. 2006....
Cyanobacterial ecotypes in the microbial mat community of Mushroom Spring (Yellowstone National Park, Wyoming) as species-like units linking microbial community composition, structure and functionDavid M Ward
Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, MT 59717, USA
Philos Trans R Soc Lond B Biol Sci 361:1997-2008. 2006..Further, we show that increased molecular resolution enhances our ability to detect ecotypes in this way, though yet higher molecular resolution is probably needed to detect all ecotypes in this microbial community...
Fine-scale distribution patterns of Synechococcus ecological diversity in microbial mats of Mushroom Spring, Yellowstone National ParkEric D Becraft
Department of Land Resources and Environmental Sciences, Montana State University, 334 Leon Johnson Hall, Bozeman, MT 59715, USA
Appl Environ Microbiol 77:7689-97. 2011..Different distributions along effluent channel flow and vertical gradients, where temperature, light, and O₂ concentrations are known to vary, confirmed the ecological distinctness of putative ecotypes...
Assessing soil microbial populations responding to crude-oil amendment at different temperatures using phylogenetic, functional gene (alkB) and physiological analysesNatsuko Hamamura
Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, MT 59717, USA
Environ Sci Technol 42:7580-6. 2008..High resolution analysis of alkB genes enabled the differentiation of distinct alkane-degrading populations responding to crude-oil amendment from other closely related, well-studied strains with different temperature adaptations...
Community ecology of hot spring cyanobacterial mats: predominant populations and their functional potentialChristian G Klatt
Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, MT 59717, USA
ISME J 5:1262-78. 2011..There was evidence of horizontal gene transfer among native populations, but the frequency of these events was inversely proportional to phylogenetic relatedness...
Geographical isolation in hot spring cyanobacteriaR Thane Papke
Department of Microbiology, 109 Lewis Hall, Montana State University, Bozeman, MT 59717, USA
Environ Microbiol 5:650-9. 2003..Thus, geographical isolation (i.e. genetic drift) must in part be responsible for driving the observed evolutionary divergences. Geographical isolation may be an important underestimated aspect of microbial evolution...
Effect of temperature and light on growth of and photosynthesis by Synechococcus isolates typical of those predominating in the octopus spring microbial mat community of Yellowstone National ParkJessica P Allewalt
Land Resources and Environmental Sciences, Montana State University, P.O. Box 3120, Bozeman, MT 59717-3120, USA
Appl Environ Microbiol 72:544-50. 2006..Growth rate and photosynthetic responses provided evidence for light acclimation but evidence of, at best, only subtle light adaptation...
Microbial population dynamics associated with crude-oil biodegradation in diverse soilsNatsuko Hamamura
Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, MT 59717, USA
Appl Environ Microbiol 72:6316-24. 2006..However, similar Rhodococcus erythropolis-like populations were observed in four of the seven soils and were the most common hydrocarbon-degrading organisms identified via cultivation...
Influence of molecular resolution on sequence-based discovery of ecological diversity among Synechococcus populations in an alkaline siliceous hot spring microbial matMelanie C Melendrez
Land Resources and Environmental Science, Montana State University, Bozeman, Montana, USA
Appl Environ Microbiol 77:1359-67. 2011..Many ecotypes hypothesized by sequence analyses were different in their habitat specificities, suggesting different adaptations to temperature or other parameters that vary along the flow channel...
In silico approaches to study mass and energy flows in microbial consortia: a syntrophic case studyReed Taffs
Thermal Biology Institute, Montana State University, Bozeman, MT 59717, USA
BMC Syst Biol 3:114. 2009..Two phases, day and night, were modeled to account for differences in the sources of mass and energy and the routes available for their exchange...
Diversity and functional analysis of bacterial communities associated with natural hydrocarbon seeps in acidic soils at Rainbow Springs, Yellowstone National ParkNatsuko Hamamura
Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, MT 59717, USA
Appl Environ Microbiol 71:5943-50. 2005..This and the presence of an alkB gene homolog in this isolate confirmed the alkane degradation capability of one population indigenous to acidic hydrocarbon seep soils...
Microscopic examination of distribution and phenotypic properties of phylogenetically diverse Chloroflexaceae-related bacteria in hot spring microbial matsUlrich Nubel
Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, Montana, USA
Appl Environ Microbiol 68:4593-603. 2002..Both type C organisms and Chloroflexus spp. were observed to assimilate radiolabeled acetate under in situ conditions...
The importance of physical isolation to microbial diversificationR Thane Papke
Department of Land Resources and Environmental Studies, Montana State University, Bozeman, 59715, USA
FEMS Microbiol Ecol 48:293-303. 2004..Here we review the theoretical paradigm of physical isolation for the diversification of organisms in general and then provide a variety of evidence indicating that microbial populations also fit into a similar evolutionary framework...
Comparative genomics provides evidence for the 3-hydroxypropionate autotrophic pathway in filamentous anoxygenic phototrophic bacteria and in hot spring microbial matsChristian G Klatt
Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, MT, USA
Environ Microbiol 9:2067-78. 2007..were participating in photoheterotrophic uptake of cyanobacterial photosynthate produced by the reductive pentose phosphate cycle...
Competitive fitness of isolates enriched on phenanthrene sorbed to model phasesGregory M Colores
Department of Land Resources and Environmental Sciences, Montana State University, Bozeman 59717 3120, USA
Appl Environ Microbiol 73:4074-7. 2007..Our results indicate that contaminant availability alone does not determine the isolates' competitive fitness...
Cyanobacterial ecotypes in different optical microenvironments of a 68 degrees C hot spring mat community revealed by 16S-23S rRNA internal transcribed spacer region variationMike J Ferris
Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, MT 59717 3120, USA
Appl Environ Microbiol 69:2893-8. 2003....
Complete genome of Candidatus Chloracidobacterium thermophilum, a chlorophyll-based photoheterotroph belonging to the phylum AcidobacteriaAmaya M Garcia Costas
Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
Environ Microbiol 14:177-90. 2012..Some transposases were homologous to those of mat community members from other phyla...
Cultivation and genomic, nutritional, and lipid biomarker characterization of Roseiflexus strains closely related to predominant in situ populations inhabiting Yellowstone hot spring microbial matsMarcel T J van der Meer
NIOZ Royal Netherlands Institute for Sea Research, Department of Marine Organic Biogeochemistry, P O Box 59, 1790 AB Den Burg, Texel, Netherlands
J Bacteriol 192:3033-42. 2010..The isolates exhibit temperature, pH, and sulfide preferences typical of their habitat. Lipids produced by these isolates matched much better with mat lipids than do lipids produced by R. castenholzii or Chloroflexus isolates...
Archaeal and bacterial glycerol dialkyl glycerol tetraether lipids in hot springs of yellowstone national parkStefan Schouten
Department of Marine Biogeochemistry and Toxicology, Royal Netherlands Institute for Sea Research, P O Box 59, 1790 AB Den Burg, Texel, The Netherlands
Appl Environ Microbiol 73:6181-91. 2007..Finally, the distribution of bacterial branched GDGTs suggests that they may be derived from the geothermally heated soils surrounding the hot springs...
Diel variations in carbon metabolism by green nonsulfur-like bacteria in alkaline siliceous hot spring microbial mats from Yellowstone National ParkMarcel T J van der Meer
Department of Marine Biogeochemistry and Toxicology, The Royal Netherlands Institute for Sea Research, P O Box 59, 1790 AB Den Burg Texel, The Netherlands
Appl Environ Microbiol 71:3978-86. 2005..In addition, we observed direct incorporation of [13C]acetate into GNSLB lipids in the morning. This suggests that GNSLB also have a potential for photoheterotrophy in situ...
Impact of carbon metabolism on 13C signatures of cyanobacteria and green non-sulfur-like bacteria inhabiting a microbial mat from an alkaline siliceous hot spring in Yellowstone National Park (USA)Marcel T J van der Meer
Royal Netherlands Institute for Sea Research NIOZ, Department of Marine Biogeochemistry and Toxicology, PO Box 59, 1790 AB Den Burg, Texel, The Netherlands
Environ Microbiol 9:482-91. 2007..The assimilation of 13C-enriched cyanobacterial carbon may thus lead to enriched 13C-contents of GNSLB cell components...
Regulation of nif gene expression and the energetics of N2 fixation over the diel cycle in a hot spring microbial matAnne Soisig Steunou
Department of Plant Biology, The Carnegie Institution, Stanford University, Stanford, CA, USA
ISME J 2:364-78. 2008..These results are discussed with respect to the energetics and regulation of N2 fixation in hot spring mats and factors that can markedly influence the extent of N2 fixation over the diel cycle...
Population level functional diversity in a microbial community revealed by comparative genomic and metagenomic analysesDevaki Bhaya
Department of Plant Biology, Carnegie Institution, Stanford, CA 94305, USA
ISME J 1:703-13. 2007....
Candidatus Chloracidobacterium thermophilum: an aerobic phototrophic AcidobacteriumDonald A Bryant
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, USA
Science 317:523-6. 2007..quot;Candidatus Chloracidobacterium thermophilum" is a BChl-producing member of the poorly characterized phylum Acidobacteria...
Alkane-1,2-diol-based glycosides and fatty glycosides and wax esters in Roseiflexus castenholzii and hot spring microbial matsMarcel T J van ver Meer
Netherlands Institute for Sea Research NIOZ, Department of Marine Biogeochemistry and Toxicology, P O Box 59, 1790 AB Den Burg, Texel, The Netherlands
Arch Microbiol 178:229-37. 2002..In lipid extracts from two nonsulfidic hot spring microbial mats, similar alkane-1,2-diol-based lipids were detected in minor amounts. R. castenholzii lipids are compared to lipids of mats and other thermophilic mat isolates...
Identifying the fundamental units of bacterial diversity: a paradigm shift to incorporate ecology into bacterial systematicsAlexander Koeppel
Departments of Biology and Mathematics and Computer Science, Wesleyan University, Middletown, CT 06459, USA
Proc Natl Acad Sci U S A 105:2504-9. 2008..Ecotype simulation provides a long-needed natural foundation for microbial ecology and systematics...
In situ analysis of nitrogen fixation and metabolic switching in unicellular thermophilic cyanobacteria inhabiting hot spring microbial matsAnne Soisig Steunou
Department of Plant Biology, The Carnegie Institution, 260 Panama Street, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 103:2398-403. 2006..In a broader context, our data suggest that there are critical regulatory switches in situ that are linked to the diel cycle and that these switches alter many metabolic processes within the microbial mat...
Compound-specific isotopic fractionation patterns suggest different carbon metabolisms among Chloroflexus-like bacteria in hot-spring microbial matsMarcel T J van der Meer
Department of Marine Biogeochemistry and Toxicology, Royal Netherlands Institute for Sea Research, 1790 AB Den Burg, Texel, The Netherlands
Appl Environ Microbiol 69:6000-6. 2003....
