David Pascual

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..These studies show that impairment of L-Sel(-/-) DC maturation results in insufficient mature DCs that require microbial activation to restore increases in respiratory CD8(+) DCs to support CTL responses...

..Thus, these data show that T(reg) cells are induced by oral vaccination with Salmonella-CFA/I contributing to the efficacious treatment of autoimmune disease...

..These studies show that HNLN B cells express alpha(E)beta(7) in the absence of L-selectin to sustain distal IgA responses...

..This biphasic response offers an alternative strategy for directing Salmonella vector-induced host immunity along a Th2 cell-dependent pathway, allowing for early promotion of mucosal and systemic Abs...

..Thus, these studies suggest that SP mediation contributes to proinflammatory responses to Salmonella infections...

..Nonetheless, an understanding of how some pathogens have evolved to circumvent interleukin-18 and of how interleukin-18 can be effectively induced are essential to our ability to develop better vaccines against enteric pathogens...

..This evidence shows that the expression of CFA/I fimbriae alters how macrophages recognize or process salmonellae and prevents the rapid onset of proinflammatory cytokines which is typical during Salmonella infections...

..Collectively, these results show that oral administration of APP protects against experimental colitis and diminishes proinflammatory cytokine expression via T cells...

..Ad2-restimulated cells showed bias toward IL-2 and IFN-gamma, while betagal-restimulated cells showed bias toward IL-4 and IL-10...

..These results show that IL-12 can be used as a molecular adjuvant to enhance protective immunity against pneumonic plague, but in a dose-dependent fashion...

..Thus, our data show that NMII is capable of eliciting disease in immunocompromised mice, which may help in evaluation of vaccine candidates as well as the study of host-pathogen interactions...

..Such co-stimulation also favored the production of IFN-gamma and IL-10 where CD4(+) T cells were more inclined to produce IFN-gamma and IL-2...

..Yet, these increases in CONALT AFC correlated with restoration of immunity in L-Sel(-/-) nasal passages and reproductive tracts...

..m. immunization. These results show that LTN can be used as a molecular adjuvant to enhance protective immunity against plague...

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..When immunized mice were challenged with B. melitensis 16M, bp26-plus Tf-immunized mice showed no difference in splenic weights but harbored three-fold less bacterial CFU when compared to sPBS-immunized control mice...

..Thus, these studies showed that retention of K99 fimbrial subunit as native fimbriae or with the deletion of fanGH is sufficient to confer protection...

..Thus, compared with live vectors, protein sigma1-mediated DNA delivery represents an alternative mucosal formulation for inducing cellular immunity against HIV-1...

..These collective results suggest that both bp26 and TF are excellent candidates for use in future vaccination studies against brucellosis...

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..abortus 2308 conferred 0.79 log protection, similar to S19 as did a single dose of DeltaznuA B. abortus 2308. Thus, this study shows the DeltaznuA DeltapurE B. abortus 2308 strain to be a potential livestock vaccine candidate...

..IL-35 treatments of IL-10-/- mice failed to induce protective CD39+CD4+ T cells, demonstrating the effector role of IL-10 by IL-35 immunosuppression...

..Likewise, elevated envelope-specific CTLs were induced. Thus, UEA-1 mediated DNA delivery represents an alternative mucosal formulation for inducing humoral and cellular immunity against HIV-1...

..These results show that IL-12 can be used as a molecular adjuvant to enhance protective immunity against pneumonic plague...

..These data demonstrate the feasibility of using psigma1 as a mucosal delivery platform specifically for low-dose tolerance induction...

..Importantly, these T(reg) cells can be induced to high potency by simply vaccinating against irrelevant Ags, offering a novel approach to treat autoimmune diseases independently of the autoantigen...

..The microarrays of living S. Typhimurium cells immobilized in this manner remained viable and effective for at least 2 weeks in growth medium before a thick biofilm covered the whole surface...

..Together with gene expression trends in vivo, these data support early priming activation of target tissue gammadelta T cells during Salmonella serovar Typhimurium infection...

..Protection studies confirmed the DeltaznuA mutant as a potential live vaccine, since protection against wild-type B. abortus 2308 challenge was as effective as that obtained with the RB51 or S19 vaccine strain...

..These data suggest that oral immunization with the live, attenuated ΔznuA B. melitensis vaccine provides an attractive strategy to protect against inhalational infection with virulent B. melitensis...

..Thus, psigma1-based therapy can resolve EAE independently of or dependently upon CD25 and assigns IL-28 as an alternative therapy for autoimmunity...

..Thus, tachykinins introduce new circuits to immune regulation suggesting that these neuropeptides exhibit cytokine- and chemokine-like action...

..The results are explained on the basis of the concept of the self-repairing of lipid bilayers and the peptidoglycan layer...

..This study shows that the Hcbetatre structure can confer protective immunity and that use of Hcbetatre-Ad2F gives more rapid and sustained mucosal and plasma Ab responses...

..These data show that Salmonella-CFA/I vaccination of DBA/1 mice protects against collagen-induced arthritis by stimulating TGF-beta- and IL-4-producing regulatory CD4(+) T cells...

..pestis. These results show that a single Salmonella vaccine can deliver both F1- and V-Ags to effect both systemic and mucosal immune protection against Y. pestis...

..This method was successfully used to sort a targeted bacterial species from a mixed culture within 2 h...

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..In this study, we examined whether M-cell targeting using a protein antigen (Ag) delivery system would induce oral tolerance instead of enhancement of Ag-specific mucosal antibody (Ab) responses...

..In this article, we will discuss the importance of the development of a mucosal vaccine against botulinum and introduce current aspects of BoNT mucosal vaccines, which show that they effectively prevent mucosal BoNT intoxication...

..These experiments are the first to demonstrate the expression and immunogenicity of a model subunit antigen in the soybean system, and mark the first steps toward the development of a K99 edible vaccine to protect against ETEC...

..The fecal extract samples from nasally vaccinated mice were found to contain neutralizing secretory IgA Abs. Taken together, these results show that nasal BoNT/A vaccine effectively prevents mucosal BoNT intoxication...

..These data show that T(reg) cells can be induced to high potency to non-disease-inducing Ags using a bacterial vaccine...

..pestis challenge. From these studies, we will produce efficacious vaccines to plague, as well as a supporting vaccine regimen, that will confer protective immunity in the mucosal and systemic immune compartments. ..

..Thus, from these studies we will learn the specific mechanisms induced by this anti-inflammatory vaccine. ..

..n. challenge with CT. From this work, we will learn how routes of immunizations can be used to effectively target vaccines to nonintestinal mucosal sites. ..

..This application is responsive to RFA-AT-06-005: "Mechanisms of Immune Modulation" by addressing the requested research areas in understanding CAMs' mechanisms of action upon the induction and expansion of T cell regulatory populations. ..

..Thus, from these studies we will learn the specific mechanisms induced by this anti-inflammatory vaccine. ..

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..These studies will provide the basis for future development of subunit vaccines to target the NALT for eliciting protective immunity via the common mucosal immune system. ..

..These studies will provide the basis for future development of subunit vaccines to target the NALT for eliciting protective immunity in the oral cavity and SALT. ..

..Thus, once we are able to establish the ps1 delivery technology, we will move forward to use current models for Sj"gren's Syndrome to test if disease progression can be reduced. ..