Research Topics
| K R GeeSummaryAffiliation: Molecular Probes Country: USA Publications
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Detail Information
Publications
Chemical and physiological characterization of fluo-4 Ca(2+)-indicator dyesK R Gee
Molecular Probes, Inc, Eugene, OR 97402, USA
Cell Calcium 27:97-106. 2000..Because of its higher fluorescence emission intensity, fluo-4 can be used at lower intracellular concentrations, making its use a less invasive practice...
Redox-dependent trafficking of 2,3,4,5, 6-pentafluorodihydrotetramethylrosamine, a novel fluorogenic indicator of cellular oxidative activityC S Chen
Molecular Probes, Inc, Eugene, OR 97402, USA
Free Radic Biol Med 28:1266-78. 2000..Based on its subcellular distribution and its oxidation by a broad range of oxidizing species, PF-H(2)TMRos is demonstrated to be a novel indicator for cellular oxidative stresses...
New ratiometric fluorescent calcium indicators with moderately attenuated binding affinitiesK R Gee
Molecular Probes, Inc, Eugene, OR 97402, USA
Bioorg Med Chem Lett 10:1515-8. 2000..Two new excitation ratioable fluorescent calcium indicators, benzothiaza-1 and 2, were also synthesized. Kd values of 400 nM to 5.3 microM [Ca2+] were observed in these families of new probes...
Synthesis of novel fluorinated coumarins: excellent UV-light excitable fluorescent dyesW C Sun
Molecular Probes, Inc, Eugene, Oregon 97402 9165, USA
Bioorg Med Chem Lett 8:3107-10. 1998..63 to 0.89) equal to or higher than that of the parent compound (0.63), which, in combination with their lower pKaS and higher photostability, make them superior fluorescent dyes for use as reporter molecules in biological systems...
Caged Q-rhodamine dextran: a new photoactivated fluorescent tracerK R Gee
Molecular Probes, Inc, 4849 Pitchford Avenue, Eugene, OR 97402, USA
Bioorg Med Chem Lett 11:2181-3. 2001..The resulting fluorescent cell was imaged by fluorescence microscopy through a single round of cell division...
Measuring zinc in living cells. A new generation of sensitive and selective fluorescent probesK R Gee
Molecular Probes, Inc, 4849 Pitchford Avenue, Eugene, OR 97478, USA
Cell Calcium 31:245-51. 2002..Experiments in neuronal and non-neuronal cell cultures show the new indicators to retain their sensitivity to and selectivity for zinc after conversion to cell-permeable forms...
Control of NO concentration in solutions of nitrosothiol compounds by lightV R Zhelyaskov
World Precision Instruments, Inc, International Trade Center, Sarasota, FL 34240 9258, USA
Photochem Photobiol 67:282-8. 1998..Generation of superoxide as a product of a photolytic decomposition reaction of S-nitrosothiols and further oxidation of NO is the most likely mechanism for light suppression of NO concentration...
Fluorescent Bocillins: synthesis and application in the detection of penicillin-binding proteinsK R Gee
Molecular Probes, Inc, Eugene, OR 97402, USA
Electrophoresis 22:960-5. 2001..These reagents allow for facile detection of 2-4 ng of purified PBP with the aid of a fluorescent scanner...
Fluorescent BODIPY-GTP analogs: real-time measurement of nucleotide binding to G proteinsD P McEwen
Department of Pharmacology, University of Michigan, Ann Arbor, MI 48109-0632, USA
Anal Biochem 291:109-17. 2001..The visible excitation and emission spectra and high fluorescence levels of these probes permit robust real-time detection of nucleotide binding...
