Stephen Pruett

Summary

Affiliation: Mississippi State University
Country: USA

Publications

  1. pmc Transcriptomic analysis of peritoneal cells in a mouse model of sepsis: confirmatory and novel results in early and late sepsis
    Minny Bhatty
    Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA
    BMC Genomics 13:509. 2012
  2. pmc Oxidative stress and sodium methyldithiocarbamate-induced modulation of the macrophage response to lipopolysaccharide in vivo
    Stephen B Pruett
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71103, USA
    Toxicol Sci 109:237-46. 2009
  3. pmc Patterns of immunotoxicity associated with chronic as compared with acute exposure to chemical or physical stressors and their relevance with regard to the role of stress and with regard to immunotoxicity testing
    Stephen B Pruett
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130, USA
    Toxicol Sci 109:265-75. 2009
  4. pmc Innate immunity and inflammation in sepsis: mechanisms of suppressed host resistance in mice treated with ethanol in a binge-drinking model
    Stephen B Pruett
    Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi 39762, USA
    Toxicol Sci 117:314-24. 2010
  5. pmc Ethanol inhibits LPS-induced signaling and modulates cytokine production in peritoneal macrophages in vivo in a model for binge drinking
    Stephen B Pruett
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA
    BMC Immunol 10:49. 2009
  6. pmc The role of stress mediators in modulation of cytokine production by ethanol
    Mitzi Glover
    LSU Health Sciences Center, Department of Cellular Biology and Anatomy, Shreveport, LA 71130, USA
    Toxicol Appl Pharmacol 239:98-105. 2009
  7. ncbi request reprint Modeling and predicting immunological effects of chemical stressors: characterization of a quantitative biomarker for immunological changes caused by atrazine and ethanol
    Stephen B Pruett
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130, USA
    Toxicol Sci 75:343-54. 2003
  8. ncbi request reprint Modeling and predicting stress-induced immunosuppression in mice using blood parameters
    Carlton L Schwab
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130, USA
    Toxicol Sci 83:101-13. 2005
  9. ncbi request reprint Sodium methyldithiocarbamate inhibits MAP kinase activation through toll-like receptor 4, alters cytokine production by mouse peritoneal macrophages, and suppresses innate immunity
    Stephen B Pruett
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130, USA
    Toxicol Sci 87:75-85. 2005
  10. ncbi request reprint Involvement of three mechanisms in the alteration of cytokine responses by sodium methyldithiocarbamate
    Stephen B Pruett
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, 71130, USA
    Toxicol Appl Pharmacol 213:172-8. 2006

Research Grants

Collaborators

Detail Information

Publications28

  1. pmc Transcriptomic analysis of peritoneal cells in a mouse model of sepsis: confirmatory and novel results in early and late sepsis
    Minny Bhatty
    Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA
    BMC Genomics 13:509. 2012
    ..A microarray-based approach was used to study the temporal changes in cells from the peritoneal cavity of septic mice and to identify potential biomarkers and therapeutic targets for this subset of sepsis patients...
  2. pmc Oxidative stress and sodium methyldithiocarbamate-induced modulation of the macrophage response to lipopolysaccharide in vivo
    Stephen B Pruett
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71103, USA
    Toxicol Sci 109:237-46. 2009
    ..However, oxidative stress alone cannot explain the increased IL-10 production caused by SMD...
  3. pmc Patterns of immunotoxicity associated with chronic as compared with acute exposure to chemical or physical stressors and their relevance with regard to the role of stress and with regard to immunotoxicity testing
    Stephen B Pruett
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130, USA
    Toxicol Sci 109:265-75. 2009
    ..These results indicate that the nature of the stressor determines whether mice become tolerant with regard to the stress response and consequent immunological effects. This finding has practical implications for safety testing in mice...
  4. pmc Innate immunity and inflammation in sepsis: mechanisms of suppressed host resistance in mice treated with ethanol in a binge-drinking model
    Stephen B Pruett
    Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi 39762, USA
    Toxicol Sci 117:314-24. 2010
    ..Thus, the effects of ethanol responsible for lethal outcome in sepsis are not dependent on inhibition of TLR4 signaling, as we and others had previously suspected...
  5. pmc Ethanol inhibits LPS-induced signaling and modulates cytokine production in peritoneal macrophages in vivo in a model for binge drinking
    Stephen B Pruett
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA
    BMC Immunol 10:49. 2009
    ....
  6. pmc The role of stress mediators in modulation of cytokine production by ethanol
    Mitzi Glover
    LSU Health Sciences Center, Department of Cellular Biology and Anatomy, Shreveport, LA 71130, USA
    Toxicol Appl Pharmacol 239:98-105. 2009
    ....
  7. ncbi request reprint Modeling and predicting immunological effects of chemical stressors: characterization of a quantitative biomarker for immunological changes caused by atrazine and ethanol
    Stephen B Pruett
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130, USA
    Toxicol Sci 75:343-54. 2003
    ..One of the parameters (MHC class II expression) was evaluated as a predictive biomarker for stress-related immunosuppression, and the results suggest it could be suitable for that purpose...
  8. ncbi request reprint Modeling and predicting stress-induced immunosuppression in mice using blood parameters
    Carlton L Schwab
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130, USA
    Toxicol Sci 83:101-13. 2005
    ..Nevertheless, MHC class II expression seems to provide a reasonably consistent indication of stress exposure in blood and spleen...
  9. ncbi request reprint Sodium methyldithiocarbamate inhibits MAP kinase activation through toll-like receptor 4, alters cytokine production by mouse peritoneal macrophages, and suppresses innate immunity
    Stephen B Pruett
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130, USA
    Toxicol Sci 87:75-85. 2005
    ..Treatment of mice with SMD decreased survival following challenge with non-pathogenic Escherichia coli within 24-48 h, demonstrating suppression of innate immunity...
  10. ncbi request reprint Involvement of three mechanisms in the alteration of cytokine responses by sodium methyldithiocarbamate
    Stephen B Pruett
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, 71130, USA
    Toxicol Appl Pharmacol 213:172-8. 2006
    ..This treatment partially prevented the action of SMD on IL-10 and IL-12 in the peritoneal fluid. The results suggest that all of the proposed mechanisms have some role in the alteration of cytokine production by SMD...
  11. pmc The role of glucocorticoids in the immediate vs. delayed effects of acute ethanol exposure on cytokine production in a binge drinking model
    Mitzi Glover
    LSU Health Sciences Center, Shreveport, Louisiana, USA
    Int Immunopharmacol 11:755-61. 2011
    ..The purpose of this study was to determine the role of corticosterone on these effects. Also, since gender differences occur in immune responses, separate experiments were performed using male and female mice...
  12. ncbi request reprint Characterization of glucocorticoid receptor translocation, cytoplasmic IkappaB, nuclear NFkappaB, and activation of NFkappaB in T lymphocytes exposed to stress-inducible concentrations of corticosterone in vivo
    Stephen B Pruett
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71130, USA
    Int Immunopharmacol 3:1-16. 2003
    ..Additionally, upregulation of IkappaB and consequent inhibition of NFkappaB activation are not prominent in vivo, in contrast to results obtained in vitro or with a synthetic glucocorticoid...
  13. ncbi request reprint Characterization of the action of drug-induced stress responses on the immune system: evaluation of biomarkers for drug-induced stress in rats
    Stephen Pruett
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, Shreveport, Louisiana, USA
    J Immunotoxicol 4:25-38. 2007
    ..However, the good correlation between urine and serum corticosterone levels suggest that urine corticosterone may be a potential biomarker of stress induced changes to the immune response...
  14. doi request reprint Urinary corticosterone as an indicator of stress-mediated immunological changes in rats
    Stephen Pruett
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130, USA
    J Immunotoxicol 5:17-22. 2008
    ..Because these results can be obtained reliably during routine preclinical evaluations, they should be useful for the weight-of-evidence approaches often used in regulatory settings...
  15. ncbi request reprint Role of corticosterone in immunosuppressive effects of acute ethanol exposure on Toll-like receptor mediated cytokine production
    Mitzi Glover
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, LSUHSC S, 1501 Kings Highway, Shreveport, LA 71130, USA
    J Neuroimmune Pharmacol 1:435-42. 2006
    ....
  16. ncbi request reprint Differences in IL-10 and IL-12 production patterns and differences in the effects of acute ethanol treatment on macrophages in vivo and in vitro
    Stephen B Pruett
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA
    Alcohol 37:1-8. 2005
    ..This suggests that results for cytokine studies and probably signaling studies as well that are conducted in vitro should be interpreted with caution and confirmed in vivo, particularly if they involve IL-10 and IL-12...
  17. ncbi request reprint Suppression of innate immunity by acute ethanol administration: a global perspective and a new mechanism beginning with inhibition of signaling through TLR3
    Stephen B Pruett
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA
    J Immunol 173:2715-24. 2004
    ....
  18. ncbi request reprint Ethanol suppresses LPS-induced Toll-like receptor 4 clustering, reorganization of the actin cytoskeleton, and associated TNF-alpha production
    Qun Dai
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130 3932, USA
    Alcohol Clin Exp Res 30:1436-44. 2006
    ..We recently found that acute EtOH alters lipopolysaccharide (LPS)-induced partitioning of CD14, a critical component of the LPS receptor complex, within lipid raft fractions in the macrophage-like cell line RAW264.7...
  19. ncbi request reprint Acute ethanol administration profoundly alters poly I:C-induced cytokine expression in mice by a mechanism that is not dependent on corticosterone
    Stephen B Pruett
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, 1501 Kings Hwy, Shreveport, LA 71130, USA
    Life Sci 72:1825-39. 2003
    ..These results plus previously published findings are consistent with the idea that EtOH may be a generalized suppressor of toll-like receptor signaling...
  20. ncbi request reprint Ethanol decreases natural killer cell activation but only minimally affects anatomical distribution after administration of polyinosinic:polycytidylic acid: role in resistance to B16F10 melanoma
    Pamela Hebert
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130, USA
    Alcohol Clin Exp Res 27:1622-31. 2003
    ..This effect is reduced after administration of ethanol (EtOH; 6 g/kg by oral gavage). The present study was conducted to determine whether decreased resistance is due to alteration of the distribution and/or the activation of NK cells...
  21. ncbi request reprint Ethanol suppresses polyinosinic:polycytidylic acid-induced activation of natural killer cells primarily by acting on natural killer cells, not through effects on other cell types
    Pamela Hebert
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71130, USA
    Alcohol 28:75-81. 2002
    ..1 monoclonal antibody, were used instead of perforin knockout mice. These results indicate that ethanol suppresses activation of NK cells primarily by suppressing the NK cell response to poly I:C, not by acting on another cell type...
  22. ncbi request reprint Assessment of immunologic mechanisms for flare reactions to Synvisc
    Andrew A Marino
    Department of Orthopaedic Surgery, Louisiana State University Health Sciences Center, Shreveport, LA 71130 3932, USA
    Clin Orthop Relat Res 442:187-94. 2006
    ..Flares that sometimes occur after treatment with Synvisc are probably not Type-1 (antibody-mediated) hypersensitivity reactions, but may be Type-4 (cell-mediated) hypersensitivity reactions...
  23. ncbi request reprint Ethanol suppresses cytokine responses induced through Toll-like receptors as well as innate resistance to Escherichia coli in a mouse model for binge drinking
    Stephen B Pruett
    Department of Cellular Biology and Anatomy, LSU Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71130, USA
    Alcohol 33:147-55. 2004
    ..Ethanol significantly decreased host resistance to E. coli peritonitis. Thus, ethanol suppresses responses induced by TLR receptors in mice and in the same experimental system it suppresses resistance to infection...
  24. ncbi request reprint Ethanol alters cellular activation and CD14 partitioning in lipid rafts
    Qun Dai
    Department of Cellular Biology and Anatomy, Louisiana State University Health Science Center, 1501 Kings Highway, Shreveport, LA 71130, USA
    Biochem Biophys Res Commun 332:37-42. 2005
    ..This membrane action of EtOH might be one of the mechanisms by which EtOH acts as a generalized suppressor for TLR signaling...
  25. pmc An explanation for the paradoxical induction and suppression of an acute phase response by ethanol
    Brandon S Pruett
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71130, USA
    Alcohol 39:105-10. 2006
    ..These results provide a resolution for the apparently paradoxical pro- and anti-inflammatory actions of EtOH with regard to acute phase responses...
  26. pmc Dendritic cells are required for optimal activation of natural killer functions following primary infection with herpes simplex virus type 1
    Sadik H Kassim
    Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130, USA
    J Virol 83:3175-86. 2009
    ..Overall, this study provides direct evidence that functional DCs are required for optimal IFN-gamma expression and cytolytic function by NK cells following infection with HSV-1...
  27. pmc Thymus-derived glucocorticoids are insufficient for normal thymus homeostasis in the adult mouse
    Stephen B Pruett
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA, USA
    BMC Immunol 5:24. 2004
    ..Reversal of the increased number of thymocytes caused by adrenalectomy following restoration of physiological corticosterone concentrations would indicate that corticosterone is the major adrenal product involved in thymic homeostasis...
  28. ncbi request reprint Acute exposure to ethanol affects Toll-like receptor signaling and subsequent responses: an overview of recent studies
    Stephen B Pruett
    Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71130, USA
    Alcohol 33:235-9. 2004
    ..Thus, ethanol alters responses through most or all mouse TLRs, and this suppresses expression of a wide range of innate immune mediators...

Research Grants22

  1. Mechanisms of Immunotoxcity of Chemical Stressors
    Stephen Pruett; Fiscal Year: 2005
    ....
  2. MECHANISMS OF IMMUNOSUPPRESSION BY ONE DOSE OF ETHANOL
    Stephen Pruett; Fiscal Year: 2007
    ..abstract_text> ..
  3. Mechanisms of methyl dithiocarbamate immunotoxicity
    Stephen B Pruett; Fiscal Year: 2010
    ..It is also expected that the detailed study of three important mechanisms of action, which will be examined in each aim, can be generalized to other situations or toxicants that alter these parameters. ..
  4. MECHANISMS OF IMMUNOSUPPRESSION BY ONE DOSE OF ETHANOL
    Stephen Pruett; Fiscal Year: 2003
    ..These results have implications for human health, because even moderate EtOH consumption increased the incidence of cancer and many persons with EtOH-associated cancers have decreased NK cell function. ..
  5. Mechanisms of methyl dithiocarbamate immunotoxicity
    Stephen B Pruett; Fiscal Year: 2010
    ..It is also expected that the detailed study of three important mechanisms of action, which will be examined in each aim, can be generalized to other situations or toxicants that alter these parameters. ..