Research Topics
Species | James GalliganSummaryAffiliation: Michigan State University Country: USA Publications
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Publications
Signalling mechanism coupled to 5-hydroxytryptamine4 receptor-mediated facilitation of fast synaptic transmission in the guinea-pig ileum myenteric plexusJ J Galligan
Department of Pharmacology and Toxicology and the Neuroscience Program, Michigan State University, East Lansing, MI 48824, USA
Neurogastroenterol Motil 15:523-9. 2003..Protein kinase A activation leads to an increase in transmitter release from myenteric nerve terminals and a facilitation of fast excitatory synaptic transmission...
Enteric P2X receptors as potential targets for drug treatment of the irritable bowel syndromeJames J Galligan
Department of Pharmacology and Toxicology, Neuroscience Program, Life Science B440, Michigan State University, East Lansing, MI 48824, U S A
Br J Pharmacol 141:1294-302. 2004..Data on the distribution and function of P2X receptors on gastrointestinal immune cells would also provide insights into the therapeutic potential of P2X receptor agents in IBS...
Cannabinoid signalling in the enteric nervous systemJ J Galligan
Department of Pharmacology and Toxicology and the Neuroscience Program, Michigan State University, East Lansing, MI 48824, USA
Neurogastroenterol Motil 21:899-902. 2009..It is necessary that future work extends these interesting findings to intact tissues and ideally to the in vivo setting...- Pharmacology and function of nicotinic acetylcholine and P2X receptors in the enteric nervous systemJ J Galligan
Department of Pharmacology and Toxicology and the Neuroscience Program, Michigan State University, East Lansing, MI, USA
Neurogastroenterol Motil 16:64-70. 2004..P2X receptors mediating fast synaptic excitation are predominately P2X2 homomeric receptors... - Basic and clinical pharmacology of new motility promoting agentsJ J Galligan
Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48824, USA
Neurogastroenterol Motil 17:643-53. 2005..Selection of appropriate patient populations for evaluation of new drugs is also critical... - Increased substance P content in nerve fibers associated with mesenteric veins from deoxycorticosterone acetate (DOCA)-salt hypertensive ratsJames J Galligan
Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48824, USA
Regul Pept 133:97-104. 2006..NK-3 receptors mediate venoconstriction which is unchanged in DOCA-salt hypertension. Increased release of SP from perivenous nerves might contribute to the increased venomotor tone in DOCA-salt hypertension... - Endothelin receptor function in mesenteric veins from deoxycorticosterone acetate salt-hypertensive ratsRonald J Johnson
Department of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan 48824, USA
J Hypertens 20:665-76. 2002..To identify the receptors by which endothelin-1 (ET-1) increases venomotor tone in hypertension... - Impaired function of alpha2-adrenergic autoreceptors on sympathetic nerves associated with mesenteric arteries and veins in DOCA-salt hypertensionMin Luo
Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48824, USA
Am J Physiol Heart Circ Physiol 286:H1558-64. 2004..In addition, impairment of alpha(2)-adrenergic autoreceptor function in sympathetic nerves associated with arteries and veins from DOCA-salt rats results in increased NE release... - Cross-inhibition between nicotinic acetylcholine receptors and P2X receptors in myenteric neurons and HEK-293 cellsDima A Decker
Dept of Biochemistry, Michigan State Univ, East Lansing, MI 48824, USA
Am J Physiol Gastrointest Liver Physiol 296:G1267-76. 2009..In addition, active and desensitized conformations of the P2X receptor inhibit nAChR function. These molecular interactions may modulate the function of synapses that use ATP and ACh as fast synaptic transmitters in the ENS... - Increased O2*- production and upregulation of ETB receptors by sympathetic neurons in DOCA-salt hypertensive ratsXiaoling Dai
Department of Physiology, Michigan State University, East Lansing, MI 48823, USA
Hypertension 43:1048-54. 2004.... - NADPH oxidase-derived superoxide augments endothelin-1-induced venoconstriction in mineralocorticoid hypertensionLixin Li
Department of Pharmacology and Toxicology and the Neuroscience Program, Michigan State University, East Lansing, MI 48824-1317, USA
Hypertension 42:316-21. 2003..These results suggest that superoxide contributes to ET-1-induced venoconstriction through an elevated venous NADPH oxidase activity in mineralocorticoid hypertension... - Mechanisms of hypertension induced by nitric oxide (NO) deficiency: focus on venous functionKeshari M Thakali
Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48824-1317, USA
J Cardiovasc Pharmacol 47:742-50. 2006..These data suggest that while NO deficiency increases oxidative stress and sympathetic activity in both arterial and venous vessels, the impact on veins does not make a major contribution to this form of hypertension... - Differential contributions of alpha-1 and alpha-2 adrenoceptors to vasoconstriction in mesenteric arteries and veins of normal and hypertensive miceAlex A Pérez Rivera
Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48824, USA
Vascul Pharmacol 46:373-82. 2007..This contribution of alpha(2)-ARs may account for the greater sensitivity of veins compared to arteries to the contractile effects of NE... - Endothelin in the splanchnic vascular bed of DOCA-salt hypertensive ratsHong Wang
Department of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan 48824, USA
Am J Physiol Heart Circ Physiol 288:H729-36. 2005..These results argue against the hypothesis that increased venomotor tone in DOCA-salt hypertension is caused by increased ET-1 concentration around splanchnic venous smooth muscle cells... - Pharmacological properties of nicotinic acetylcholine receptors expressed by guinea pig small intestinal myenteric neuronsXiaoping Zhou
Department of Pharmacology and Toxicology, Neuroscience Program, Life Science B400, Michigan State University, East Lansing, MI 48824, USA
J Pharmacol Exp Ther 302:889-97. 2002..These subunits may be in a homogeneous population of receptors with unique pharmacological properties, or multiple receptors of different subunit composition may be expressed by individual neurons... - Alterations in sympathetic neuroeffector transmission to mesenteric arteries but not veins in DOCA-salt hypertensionJinWoo Park
Department of Chemistry, Michigan Sate University, East Lansing, MI 48824, USA
Auton Neurosci 152:11-20. 2010..alpha(2)-AR function is most prominent in MA and is impaired in DOCA-salt MA but not MV. Purinergic transmission predominates in sham MA. NE is the dominant vasoconstrictor in DOCA-salt MA and sham and DOCA-salt MV... - Endothelin-1 increases vascular superoxide via endothelin(A)-NADPH oxidase pathway in low-renin hypertensionLixin Li
Department of Pharmacology and Toxicology, Michigan State University, East Lansing 48824-1317, USA
Circulation 107:1053-8. 2003..CONCLUSIONS: These findings suggest that ET-1 augments vascular superoxide production at least in part via an ET(A)/NADPH oxidase pathway in low-renin mineralocorticoid hypertension... - Differences in sympathetic neuroeffector transmission to rat mesenteric arteries and veins as probed by in vitro continuous amperometry and video imagingJinWoo Park
Department of Chemistry and the Neuroscience Program, Michigan State University, East Lansing, MI 48824, USA
J Physiol 584:819-34. 2007..We conclude that there are fundamental differences in sympathetic neuroeffector mechanisms in MA and MV, which are likely to contribute to their different haemodynamic functions... - Nitric oxide-independent effects of tempol on sympathetic nerve activity and blood pressure in DOCA-salt ratsHui Xu
Department of Pharmacology and Toxicology and the Neuroscience Program, Michigan State University, East Lansing, Michigan 48824, USA
Am J Physiol Heart Circ Physiol 283:H885-92. 2002..There is an additional interaction between NO and tempol that contributes to depressor responses in DOCA-salt rats... - Differential alterations in sympathetic neurotransmission in mesenteric arteries and veins in DOCA-salt hypertensive ratsMin Luo
The Neuroscience Program, Michigan State University, East Lansing, MI 48824, USA
Auton Neurosci 104:47-57. 2003..In arteries, this results in an increase in the amplitude of neurogenic constrictions. In veins, increased norepinephrine release maintains neurogenic constrictions in the presence of increased NET levels... - P2X2 subunits contribute to fast synaptic excitation in myenteric neurons of the mouse small intestineJianhua Ren
Department of Pharmacology and Toxicology and the Neuroscience Program, Michigan State University, East Lansing, MI 48824, USA
J Physiol 552:809-21. 2003..Gastrointestinal transit was similar in P2X2+/+ and P2X2-/- mice. It is concluded that P2X2 homomeric receptors contribute to fEPSPs in neural pathways underlying peristalsis studied in vitro... - 5-HT4 receptor activation facilitates recovery from synaptic rundown and increases transmitter release from single varicosities of myenteric neuronsJianhua Ren
Neuroscience Program, Michigan State Univ, East Lansing, MI 48824, USA
Am J Physiol Gastrointest Liver Physiol 294:G1376-83. 2008..These responses may contribute to the prokinetic actions of 5-HT(4) receptor agonists... - P2Y2 receptors mediate ATP-induced resensitization of TRPV1 expressed by kidney projecting sensory neuronsHui Wang
Department of Physiology, Michigan State University, East Lansing, MI 48824, USA
Am J Physiol Regul Integr Comp Physiol 298:R1634-41. 2010..We conclude that P2Y(2) receptor activation can maintain TRPV1 function perhaps during sustained episodes of activity of kidney projecting sensory neurons... - Activation of ETB receptors increases superoxide levels in sympathetic ganglia in vivoYanny E Lau
Department of Pharmacology Toxicology, B327 Life Science, Michigan State University, East Lansing, MI 48824, USA
Am J Physiol Regul Integr Comp Physiol 290:R90-5. 2006..We conclude that ET(B) receptor activation in vivo significantly enhances O2- levels in sympathetic ganglia, due to both pressor effects and direct stimulation of ET(B) receptors in ganglion cells... - Peristalsis is impaired in the small intestine of mice lacking the P2X3 subunitXiaochun Bian
Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48824, USA
J Physiol 551:309-22. 2003..P2X3 subunits are localized to AH (sensory) but not S neurons. P2X3 receptors may contribute to detection of distention or intraluminal pressure increases and initiation of reflex contractions... - Pharmacological endothelin receptor interaction does not occur in veins from ET(B) receptor deficient ratsKeshari Thakali
Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48823, USA
Vascul Pharmacol 49:6-13. 2008..Our data suggest that pharmacological ET A and ET B receptor interaction may be dependent on the presence of functional ET B receptors and independent of receptor location... - Muscarinic receptors couple to modulation of nicotinic ACh receptor desensitization in myenteric neuronsErika N Brown
Dept. of Pharmacology and Toxicology, Neuroscience Program, Michigan State University, East Lansing, MI 48824, USA
Am J Physiol Gastrointest Liver Physiol 285:G37-44. 2003..This effect may involve activation of a PKC-dependent pathway. These data also suggest that nAChRs and muscarinic receptors are coupled functionally through an intracellular signaling pathway in myenteric neurons... - Impaired purinergic neurotransmission to mesenteric arteries in deoxycorticosterone acetate-salt hypertensive ratsStacie L Demel
Neuroscience Program, B328 Life Science Building, Michigan State University, East Lansing, MI 48824, USA
Hypertension 52:322-9. 2008..These data highlight the potential importance of impaired purinergic regulation of arterial tone as a target for drug treatment of hypertension... - Increased sympathetic venoconstriction and reactivity to norepinephrine in mesenteric veins in anesthetized DOCA-salt hypertensive ratsHui Xu
Department of Pharmacology and Toxicology, Neuroscience Program, Michigan State University, East Lansing, MI 48824, USA
Am J Physiol Heart Circ Physiol 293:H160-8. 2007..In DOCA-salt rats, in vivo ET-1 reactivity is maintained in MV, but reduced in MA... - Large-conductance Ca2+-activated K+ channel beta1-subunit knockout mice are not hypertensiveHui Xu
Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI, USA
Am J Physiol Heart Circ Physiol 300:H476-85. 2011..BK and L-type Ca(2+) channels do not modulate murine venous tone. It appears that selective loss of BK channel function in arteries only is not sufficient to cause sustained hypertension... - Activation of potassium channels by tempol in arterial smooth muscle cells from normotensive and deoxycorticosterone acetate-salt hypertensive ratsHui Xu
Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48824, USA
Hypertension 48:1080-7. 2006..We conclude that upregulation of the BK channel alpha-subunit protein and tempol-induced increases in BK channel P(o) contribute to the enhanced depressor response caused by tempol in DOCA-salt hypertensive rats... - Agonist actions of neonicotinoids on nicotinic acetylcholine receptors expressed by cockroach neuronsJianguo Tan
Department of Entomology, Michigan State University, East Lansing, MI 48824, USA
Neurotoxicology 28:829-42. 2007.... - Tempol lowers blood pressure and sympathetic nerve activity but not vascular O2- in DOCA-salt ratsHui Xu
Department of Pharmacology and Toxicology and the Neuroscience Program, Michigan State University, East Lansing, Mich 48824, USA
Hypertension 43:329-34. 2004..It is concluded that depressor responses and decreases in HR and RSNA caused by acute tempol treatment are caused by direct sympathetic nerve activity inhibition that is not accompanied by SOD-mimetic action in the aorta or vena cava... - Pharmacology of synaptic transmission in the enteric nervous systemJames J Galligan
Department of Pharmacology and Toxicology Life Science B440, Michigan State University, 48824, East Lansing, MI 48824, USA
Curr Opin Pharmacol 2:623-9. 2002.... - Gene transfer of endothelial NO synthase and manganese superoxide dismutase on arterial vascular cell adhesion molecule-1 expression and superoxide production in deoxycorticosterone acetate-salt hypertensionLixin Li
Department of Pharmacology, Michigan State University, East Lansing 48824-1317, USA
Arterioscler Thromb Vasc Biol 22:249-55. 2002..These results suggest that gene transfer of MnSOD or eNOS suppresses arterial VCAM-1 expression in DOCA-salt hypertension by reducing the superoxide level... - Nicotinic acetylcholine and P2X receptors in the enteric nervous systemJames J Galligan
Department of Pharmacology and Toxicology, Neuroscience Program, Life Science B440, Michigan State University, East Lansing, MI 48824, USA
Proc West Pharmacol Soc 45:231-4. 2002..Noncholinergic mechanisms of fast excitatory synaptic transmission may provide new targets for drugs that could be useful in the treatment of gastrointestinal motility disorders... - Dynamics of fast synaptic excitation during trains of stimulation in myenteric neurons of guinea-pig ileumJianhua Ren
The Neuroscience Program and the Department of Pharmacology and Toxicology, Life Science B308, Michigan State University, East Lansing, MI 48824, United States
Auton Neurosci 117:67-78. 2005..Synaptic rundown is likely due to depletion of a readily releasable pool (RRP) of neurotransmitter... - Vascular reactivity of mesenteric arteries and veins to endothelin-1 in a murine model of high blood pressureALEX A PEREZ-RIVERA
Department of Pharmacology and Toxicology, Michigan State University, East Lansing, B 440 Life Sciences Building, East Lansing, MI 48824, USA
Vascul Pharmacol 43:1-10. 2005..There was a prominent role for ET-mediated nitric oxide release in DOCA-salt but not SHAM arteries. In summary, these studies showed a differential regulation of ET-1 contractile mechanisms between murine mesenteric arteries and veins... - Alpha-1B adrenoceptors mediate neurogenic constriction in mesenteric arteries of normotensive and DOCA-salt hypertensive miceALEX A PEREZ-RIVERA
Department of Pharmacology and Toxicology, Michigan State University, B 328 Life Sciences Building, East Lansing, MI 48824, USA
Auton Neurosci 121:64-73. 2005..Nerve-released NE acts primarily at alpha1B-and to a lesser extent at alpha1A-ARs in SHAM arteries whereas NE mediates neurogenic constrictions in DOCA-salt arteries by acting at alpha1B-ARs... - Endothelin-1-induced venous contraction is maintained in DOCA-salt hypertension; studies with receptor agonistsStephanie W Watts
Department of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan 48824 1317, USA
Br J Pharmacol 137:69-79. 2002..The ET(B) receptor was present in the aorta but, unlike in veins, does not mediate contraction directly. A sustained response to ET-1 in the venous circulation may contribute to the elevated blood pressure in the DOCA-salt model... - Differential trafficking and desensitization of human ET(A) and ET(B) receptors expressed in HEK 293 cellsXiaoling Dai
Department of Pharmacology and Toxicology and Neuroscience Program, Life Science Building Room B308, Michigan State University, East Lansing, MI 48824, USA
Exp Biol Med (Maywood) 231:746-51. 2006..Finally, ET(A) and ET(B) receptors interact to change receptor trafficking which may modify ET receptor function in vascular SMCs coexpressing these receptors... - Increased reactivity of murine mesenteric veins to adrenergic agonists: functional evidence supporting increased alpha1-adrenoceptor reserve in veins compared with arteriesALEX A PEREZ-RIVERA
Department of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan 48824, USA
J Pharmacol Exp Ther 308:350-7. 2004..These data suggest that mesenteric veins express more alpha1-adrenoceptors than arteries, accounting for greater reactivity and resistance to desensitization compared with arteries... - The effects of celiac ganglionectomy on sympathetic innervation to the splanchnic organs in the ratMelissa Li
Department of Pharmacology and Toxicology, B440 Life Sciences, Michigan State University, East Lansing, MI 48824, USA
Auton Neurosci 154:66-73. 2010..Therefore, CGX is an effective means to impair sympathetic input to the splanchnic organs, but the effect of the procedure is not permanent... - Transient receptor potential vanilloid subtype 1 channel mediated neuropeptide secretion and depressor effects: role of endoplasmic reticulum associated Ca2+ release receptors in rat dorsal root ganglion neuronsWei Huang
Department of Medicine and Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan 48824, USA
J Hypertens 26:1966-75. 2008.... - Localization of NADPH oxidase in sympathetic and sensory ganglion neurons and perivascular nerve fibersXian Cao
The Neuroscience Program, Michigan State University, East Lansing, MI 48824, USA
Auton Neurosci 151:90-7. 2009..The presence of a O(2)(-*)-generating enzyme in close vicinity to the sites of neurotransmitter handling in the nerve fibers suggests the possibility of novel redox-mediated mechanisms in peripheral neurovascular control... - High mucosal serotonin availability in neonatal guinea pig ileum is associated with low serotonin transporter expressionXiaochun Bian
Department of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan 48824, USA
Gastroenterology 132:2438-47. 2007..Paracrine signaling by enterochromaffin cells (EC), which release 5-HT, has not been studied in neonates. Our aim was to compare 5-HT disposition in the intestinal mucosa of neonatal and adult guinea pigs... - Vasopressin induces vascular superoxide via endothelin-1 in mineralocorticoid hypertensionLixin Li
Department of Pharmacology and Toxicology, B403 Life Sciences Building, Michigan State University, East Lansing, MI 48824 1317, USA
Hypertension 41:663-8. 2003..These results suggest that vasopressin increases vascular superoxide levels by stimulating ET-1 formation in mineralocorticoid hypertension, and that V1-vasopressin receptors play an important role in this process... - In vitro continuous amperometry with a diamond microelectrode coupled with video microscopy for simultaneously monitoring endogenous norepinephrine and its effect on the contractile response of a rat mesenteric arteryJinWoo Park
Department of Chemistry, Department of Pharmacology and Toxicology, and the Neuroscience Program, Michigan State University, East Lansing, Michigan 48824, USA
Anal Chem 78:6756-64. 2006.... - R-type calcium channels in myenteric neurons of guinea pig small intestineXiaochun Bian
Department of Pharmacology and Toxicology, B-440 Life Science Bldg, Michigan State University, East Lansing, MI 48823, USA
Am J Physiol Gastrointest Liver Physiol 287:G134-42. 2004..The relatively positive half-activation voltage and rapid activation kinetics suggest that R-type channels could contribute to calcium entry during somal action potentials or during action potential-induced neurotransmitter release... - In vitro continuous amperometric monitoring of 5-hydroxytryptamine release from enterochromaffin cells of the guinea pig ileumBhavik Anil Patel
Department of Bioengineering, Imperial College London, London, SW7 2AZ, UK
Analyst 132:41-7. 2007..The 5-HT oxidation current increased in the presence of the serotonin transporter (SERT) inhibitor, fluoxetine (1 microM), providing evidence that the oxidation current was associated with 5-HT... - 5-hydroxytryptamine, ulcerative colitis, and irritable bowel syndrome: molecular connectionsJames J Galligan
Gastroenterology 126:1897-9. 2004 - Impaired vasodilation in response to perivascular nerve stimulation in mesenteric arteries of TRPV1-null mutant miceLi Hong Wang
Department of Cardiovascular Sciences, The First Affiliated Hospital, College of Medicine, Zhejiang University, China
J Hypertens 24:2399-408. 2006.... - Recent advances in understanding the role of serotonin in gastrointestinal motility and functional bowel disordersJames J Galligan
Neurogastroenterol Motil 19:1-4. 2007
Research Grants
- PRESYNAPTIC MECHANISMS IN THE INTESTINEJames Galligan; Fiscal Year: 2007..It is also anticipated that these studies will identify new targets for drugs that can be used to treat GI motility disorders. ..
- Experimental approaches to understanding the ENS and its disordersJames Galligan; Fiscal Year: 2006..This will help the attendees develop a network of professional contacts who can help the young investigator in the career development process. ..
- PRESYNAPTIC MECHANISMS IN THE INTESTINEJames Galligan; Fiscal Year: 2003..It is anticipated that these studies will provide new insights into mechanisms of synaptic transmission in the gut which could lead to the development of new and effect drug treatments for motility disorders. ..