Jun Wang

Summary

Affiliation: Merck Research Laboratories
Country: USA

Publications

  1. ncbi Platensimycin is a selective FabF inhibitor with potent antibiotic properties
    Jun Wang
    Merck Research Laboratories, Rahway, New Jersey 07065, USA
    Nature 441:358-61. 2006
  2. ncbi Discovery of a small molecule that inhibits cell division by blocking FtsZ, a novel therapeutic target of antibiotics
    Jun Wang
    Department of Human and Animal Infectious Disease, Merck Research Laboratories, Rahway, New Jersey 07065, USA
    J Biol Chem 278:44424-8. 2003
  3. pmc Discovery of platencin, a dual FabF and FabH inhibitor with in vivo antibiotic properties
    Jun Wang
    Merck Research Laboratories, Rahway, NJ 07065, USA
    Proc Natl Acad Sci U S A 104:7612-6. 2007
  4. ncbi Synthesis and biological evaluation of platensimycin analogs
    Hong C Shen
    Departments of Medicinal Chemistry, Merck Research Laboratories, PO Box 2000, Rahway, NJ 07065 0900, USA
    Bioorg Med Chem Lett 19:1623-7. 2009
  5. doi Platensimycin and platencin congeners from Streptomyces platensis
    Chaowei Zhang
    Merck Research Laboratories, Rahway, New Jersey 07065, USA
    J Nat Prod 74:329-40. 2011
  6. ncbi Isolation, enzyme-bound structure and antibacterial activity of platencin A1 from Streptomyces platensis
    Sheo B Singh
    Merck Research Laboratories, Rahway, NJ 07065, USA
    Bioorg Med Chem Lett 19:4756-9. 2009
  7. doi Structure and semisynthesis of platensimide A, produced by Streptomyces platensis
    Kithsiri B Herath
    Merck Research Laboratories, Rahway, NJ 07065, USA
    Org Lett 10:1699-702. 2008
  8. doi Isolation, structure and biological activities of platencin A(2)-A(4) from Streptomyces platensis
    Chaowei Zhang
    Merck Research Laboratories, Rahway, NJ 07065, USA
    Bioorg Med Chem 18:2602-10. 2010
  9. doi Isolation, structure and biological activities of platensimycin B4 from Streptomyces platensis
    Chaowei Zhang
    Merck Research Laboratories, Rahway, NJ 07065, USA
    J Antibiot (Tokyo) 62:699-702. 2009
  10. ncbi Isolation, structure, and absolute stereochemistry of platensimycin, a broad spectrum antibiotic discovered using an antisense differential sensitivity strategy
    Sheo B Singh
    Merck Research Laboratories, P O Box 2000, Rahway, New Jersey 07065, USA
    J Am Chem Soc 128:11916-20. 2006

Detail Information

Publications72

  1. ncbi Platensimycin is a selective FabF inhibitor with potent antibiotic properties
    Jun Wang
    Merck Research Laboratories, Rahway, New Jersey 07065, USA
    Nature 441:358-61. 2006
    ..Platensimycin is the most potent inhibitor reported for the FabF/B condensing enzymes, and is the only inhibitor of these targets that shows broad-spectrum activity, in vivo efficacy and no observed toxicity...
  2. ncbi Discovery of a small molecule that inhibits cell division by blocking FtsZ, a novel therapeutic target of antibiotics
    Jun Wang
    Department of Human and Animal Infectious Disease, Merck Research Laboratories, Rahway, New Jersey 07065, USA
    J Biol Chem 278:44424-8. 2003
    ....
  3. pmc Discovery of platencin, a dual FabF and FabH inhibitor with in vivo antibiotic properties
    Jun Wang
    Merck Research Laboratories, Rahway, NJ 07065, USA
    Proc Natl Acad Sci U S A 104:7612-6. 2007
    ..13 microg/ml) in S. aureus, emphasizing the fact that more antibiotics with novel structures and new modes of action can be discovered by using this antisense differential sensitivity whole-cell screening paradigm...
  4. ncbi Synthesis and biological evaluation of platensimycin analogs
    Hong C Shen
    Departments of Medicinal Chemistry, Merck Research Laboratories, PO Box 2000, Rahway, NJ 07065 0900, USA
    Bioorg Med Chem Lett 19:1623-7. 2009
    ..The medicinal chemistry efforts were focused on the modification of the enone moiety of platensimycin and several analogs showed significant activity against FabF and possess antibacterial activity...
  5. doi Platensimycin and platencin congeners from Streptomyces platensis
    Chaowei Zhang
    Merck Research Laboratories, Rahway, New Jersey 07065, USA
    J Nat Prod 74:329-40. 2011
    ....
  6. ncbi Isolation, enzyme-bound structure and antibacterial activity of platencin A1 from Streptomyces platensis
    Sheo B Singh
    Merck Research Laboratories, Rahway, NJ 07065, USA
    Bioorg Med Chem Lett 19:4756-9. 2009
    ..Isolation, structure, enzyme-bound structure and activity of platencin A(1) and two other congeners have been described...
  7. doi Structure and semisynthesis of platensimide A, produced by Streptomyces platensis
    Kithsiri B Herath
    Merck Research Laboratories, Rahway, NJ 07065, USA
    Org Lett 10:1699-702. 2008
    ..Isolation, structure, semisynthesis, and activity of these compounds are described...
  8. doi Isolation, structure and biological activities of platencin A(2)-A(4) from Streptomyces platensis
    Chaowei Zhang
    Merck Research Laboratories, Rahway, NJ 07065, USA
    Bioorg Med Chem 18:2602-10. 2010
    ..A truncated and oxidized C-13 natural congener was isolated which suggested direct intermediacy of ent-copalyl diphosphate for the biosynthesis of platensimycins and platencins...
  9. doi Isolation, structure and biological activities of platensimycin B4 from Streptomyces platensis
    Chaowei Zhang
    Merck Research Laboratories, Rahway, NJ 07065, USA
    J Antibiot (Tokyo) 62:699-702. 2009
    ..This analog showed significantly attenuated activity and critically defined the limited binding space around the aromatic ring and established the importance of the free phenolic and carboxyl group for the activity...
  10. ncbi Isolation, structure, and absolute stereochemistry of platensimycin, a broad spectrum antibiotic discovered using an antisense differential sensitivity strategy
    Sheo B Singh
    Merck Research Laboratories, P O Box 2000, Rahway, New Jersey 07065, USA
    J Am Chem Soc 128:11916-20. 2006
    ..However, cyclohexenone ring conformation renders for the stronger binding interaction with the enzyme. The isolation, structure elucidation, derivatization, and biological activity of 6,7-dihydroplatensimycin are described...
  11. ncbi Isolation and structure of platencin: a FabH and FabF dual inhibitor with potent broad-spectrum antibiotic activity
    Hiranthi Jayasuriya
    Merck Research Laboratories, P O Box 2000, Rahway, New Jersey 07065, USA
    Angew Chem Int Ed Engl 46:4684-8. 2007
  12. ncbi Discovery of bacterial fatty acid synthase inhibitors from a Phoma species as antimicrobial agents using a new antisense-based strategy
    John G Ondeyka
    Natural Products Chemistry and Medicinal Chemistry and Human and Animal Infectious Diseases, Merck Research Laboratories, Merck and Co, Inc, Rahway, New Jersey 07065, USA
    J Nat Prod 69:377-80. 2006
    ....
  13. pmc Discovery of FabH/FabF inhibitors from natural products
    Katherine Young
    Merck Research Laboratories, Rahway, NJ 07065, USA
    Antimicrob Agents Chemother 50:519-26. 2006
    ..aureus. It exhibited a spectrum of antibacterial activity against clinically important pathogens including methicillin-resistant Staphylococcus aureus, Bacillus subtilis, and Haemophilus influenzae...
  14. ncbi Determination of selectivity and efficacy of fatty acid synthesis inhibitors
    Srinivas Kodali
    Department of Human and Animal Infectious Disease, Merck Research Laboratories, Rahway, New Jersey 07065, USA
    J Biol Chem 280:1669-77. 2005
    ..2 to 0.4 microg/ml. Furthermore, the effectiveness, selectivity, and the in vitro and in vivo correlations of BABX as well as other fatty acid inhibitors were elucidated, which will aid in future drug discovery...
  15. ncbi Anthrabenzoxocinones from Streptomyces sp. as liver X receptor ligands and antibacterial agents
    Kithsiri B Herath
    Merck Research Laboratories, P O Box 2000, Rahway, New Jersey 07065, USA
    J Nat Prod 68:1437-40. 2005
    ..5-2 microg/mL). Details of the isolation, structure elucidation, LXR ligand binding, antibacterial activity, and selectivity of inhibition of 1 and 2 are described...
  16. pmc Antidiabetic and antisteatotic effects of the selective fatty acid synthase (FAS) inhibitor platensimycin in mouse models of diabetes
    Margaret Wu
    Department of Diabetes and Obesity, Merck Research Laboratories, Rahway, NJ 07065, USA
    Proc Natl Acad Sci U S A 108:5378-83. 2011
    ..PTM also reduced ambient glucose levels in db/db mice. These results provide pharmacological proof of concept of inhibiting fatty acid synthase for the treatment of diabetes and related metabolic disorders in animal models...
  17. pmc An apoA-I mimetic peptide facilitates off-loading cholesterol from HDL to liver cells through scavenger receptor BI
    Xuelei Song
    Merck Research Laboratories, Rahway, New Jersey 07065, USA
    Int J Biol Sci 5:637-46. 2009
    ..Overall our data provides evidence that D-4F is capable of mimicking apoA-I to form HDL-like particles, and off-loads cholesterol for catabolism and excretion, thus completing RCT...
  18. ncbi Highly sensitive target-based whole-cell antibacterial discovery strategy by antisense RNA silencing
    Sheo B Singh
    Merck Research Laboratories, RY80Y 350, 126 E Lincoln Avenue, Rahway, NJ 07065, USA
    Curr Opin Drug Discov Devel 10:160-6. 2007
    ....
  19. doi ApoA-I mimetic peptides promote pre-β HDL formation in vivo causing remodeling of HDL and triglyceride accumulation at higher dose
    Ester Carballo-Jane
    Merck Research Laboratories, 126 East Lincoln Avenue, RY80Y 140, Rahway, NJ 07065, USA
    Bioorg Med Chem 18:8669-78. 2010
    ..These results suggest our peptide design confers activities that are potentially anti-atherogenic. However a dosing regimen which maximizes their therapeutic properties while minimizing adverse effects needs to be established...
  20. doi Novel potent apoA-I peptide mimetics that stimulate cholesterol efflux and pre-beta particle formation in vitro
    Raffaele Ingenito
    IRBM P Angeletti, Merck Research Laboratories Peptide Centre of Excellence, Via Pontina Km 30, 600, 00040 Pomezia, Italy
    Bioorg Med Chem Lett 20:236-9. 2010
    ....
  21. pmc An apoA-I mimetic peptide increases LCAT activity in mice through increasing HDL concentration
    Xun Chen
    Merck Research Laboratories, Rahway, New Jersey 07065, USA
    Int J Biol Sci 5:489-99. 2009
    ..Finally, we have established a correlation between HDL concentration and LCAT activity in the D-4F treated mice...
  22. doi Highly enantioselective strecker reaction of ketoimines catalyzed by an organocatalyst from (S)-BINOL and L-prolinamide
    Zongrui Hou
    Key Laboratory of Green Chemistry and Technology Sichuan University, Ministry of Education, College of Chemistry, Sichuan University, Chengdu, PR China
    Chemistry 14:4484-6. 2008
  23. ncbi Ofloxacin-delivery system of a polyanhydride and polylactide blend used in the treatment of bone infection
    Liaobin Chen
    Department of Orthopaedic Surgery, Zhong Nan Hospital, Wuhan University, Wuhan 430071, People s Republic of China
    J Biomed Mater Res B Appl Biomater 83:589-95. 2007
    ..Our findings suggested that using PSA/PLA blends with different ratios as carriers for antibiotics might be useful in the treatment of chronic osteomyelitis and in the prophylaxis of bone infection...
  24. ncbi One-step, painting-like coating procedures to make surfaces highly and permanently bactericidal
    Daewon Park
    Department of Chemistry and Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, 02139, USA
    Biotechnol Prog 22:584-9. 2006
    ..This conclusion was further supported by studies in which the molecular weight of PEI and the hydrophobicity of the alkyl moiety were varied...
  25. ncbi Immobilized N-alkylated polyethylenimine avidly kills bacteria by rupturing cell membranes with no resistance developed
    Nebojsa M Milovic
    Department of Chemistry and Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Biotechnol Bioeng 90:715-22. 2005
    ..Finally, the immobilized N-alkyl-PEI, while deadly to bacteria, is determined to be harmless to mammalian (monkey kidney) cells...
  26. ncbi Inhibitory effects of fluorobenzaldehydes on the activity of mushroom tyrosinase
    Xiao hong Huang
    Key Laboratory of Biopesticide and Chemical Biology, Fujian Agriculture and Forestry University, Ministry of Education, Fuzhou 350002, PR China
    J Enzyme Inhib Med Chem 21:413-8. 2006
    ..The inhibition constants for these three fluorobenzaldehydes on the diphenolase were determined and compared...
  27. ncbi Small-molecule inhibition of TNF-alpha
    Molly M He
    Sunesis Pharmaceuticals, Incorporated, 341 Oyster Point Boulevard, South San Francisco, CA 94080, USA
    Science 310:1022-5. 2005
    ..A structure solved by x-ray crystallography reveals that a single compound molecule displaces a subunit of the trimer to form a complex with a dimer of TNF-alpha subunits...
  28. doi Otefin, a nuclear membrane protein, determines the fate of germline stem cells in Drosophila via interaction with Smad complexes
    Xiaoyong Jiang
    State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, 25 Beisihuanxi Road, Haidian, Beijing, 100080, China
    Dev Cell 14:494-506. 2008
    ..Thus, we demonstrate that specific nuclear membrane components mediate signal-dependent transcriptional effects to control stem cell behavior...
  29. ncbi Fluorescence study of DNA binding and bending by EcoRI DNA methyltransferase
    Baocheng Ma
    Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, PR China
    J Phys Chem B 110:19647-51. 2006
    ..EcoRI. The end-to-end distance of the dsDNA determined from the FRET efficiency was changed from 72.4 to 63.4 A, and the DNA bending angle was estimated as 57.8 degrees ...
  30. ncbi Argonaute 1 regulates the fate of germline stem cells in Drosophila
    Lele Yang
    State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology
    Development 134:4265-72. 2007
    ..Given that AGO1 serves as a key component of the miRNA pathway, we propose that an AGO1-dependent miRNA pathway probably plays an instructive role in repressing GSC/cystoblast differentiation...
  31. ncbi Versatile apoferritin nanoparticle labels for assay of protein
    Guodong Liu
    Pacific Northwest National Laboratory, Richland, Washington 99352, USA
    Anal Chem 78:7417-23. 2006
    ..The new nanoparticle labels hold great promise for multiplex protein detection (in connection with nanoparticles loaded with different markers) and for enhancing the sensitivity of other bioassays...
  32. ncbi GDNF acutely potentiates Ca2+ channels and excitatory synaptic transmission in midbrain dopaminergic neurons
    Jun Wang
    Institute of Neuroscience, Chinese Academy of Sciences, Shanghai, China
    Neurosignals 12:78-88. 2003
    ....
  33. ncbi Self-assembled micelles of biodegradable triblock copolymers based on poly(ethyl ethylene phosphate) and poly(-caprolactone) as drug carriers
    Yu Cai Wang
    Department of Polymer Science and Engineering, and Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, PR China
    Biomacromolecules 9:388-95. 2008
    ..These biodegradable triblock copolymer micelles are potential as novel carriers for hydrophobic drug delivery...
  34. ncbi Study of assembly of arachidic acid/LDHs hybrid films containing photoactive dyes
    Jun Wang
    Key Laboratory for Colloid and Interface Chemistry of Education Ministry, Shandong University, Jinan, Shandong 250100, People s Republic of China
    J Colloid Interface Sci 318:337-47. 2008
    ..Also, the dye molecules incorporated into AA/LDHs films exhibit excellent configuration stability under the irradiation of UV light because the LDHs matrix offers a more rigid and constrained environment for them...
  35. doi Involvement of the nitric oxide-cyclic GMP-protein kinase G-K+ channel pathway in the antihyperalgesic effects of bovine lactoferrin in a model of neuropathic pain
    Jun Wang
    Department of Anesthesiology, The First Affiliated Hospital, China Medical University, Shenyang 110001, PR China
    Brain Res 1209:1-7. 2008
    ..Data suggest that BLF-induced spinal antihyperalgesia could be due to activation of the NO-cGMP-PKG-K+ channel pathway and it is not mediated by mu-opioid receptor in a model of neuropathic pain...
  36. pmc Enhanced subunit interactions with gemcitabine-5'-diphosphate inhibit ribonucleotide reductases
    Jun Wang
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 104:14324-9. 2007
    ..This information and the proposed asymmetry between the interactions of alphanbetan provide an explanation for complete inactivation of RNR with substoichiometric amounts of F2CDP...
  37. ncbi Resveratrol suppresses prostate cancer progression in transgenic mice
    Curt E Harper
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, 1670 University Boulevard, Birmingham, AL 35294 0019, USA
    Carcinogenesis 28:1946-53. 2007
    ....
  38. ncbi Inhibitor fingerprinting of matrix metalloproteases using a combinatorial peptide hydroxamate library
    Mahesh Uttamchandani
    Department of Biological Sciences, National University of Singapore, Singapore 117543
    J Am Chem Soc 129:7848-58. 2007
    ..This approach thus paves the way forward in lead identification by providing a rapid and quantitative method for selectivity screening at the outset of the drug discovery process...
  39. doi A viscosity-tunable polymer for DNA separation by microchip electrophoresis
    Daisuke Kuroda
    Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8603, Japan
    Anal Bioanal Chem 391:2543-9. 2008
    ..To illustrate the separation mechanism of DNA in a Pluronic F127 solution, DNA molecular imaging was performed by fluorescence microscopy with F127 polymer as the separation matrix in microchip electrophoresis...
  40. doi Methyl (11aS)-1,2,3,5,11,11a-hexahydro-3,3-dimethyl-1-oxo-6H-imidazo-[3',4':1,2]pyridin[3,4-b]indol-2-substituted acetates: synthesis and three-dimensional quantitative structure-activity relationship investigation as a class of novel vasodilators
    Jiawang Liu
    College of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, PR China
    J Med Chem 51:4715-23. 2008
    ..Actually, the docking adaptabilities of these novel vasodilators to PDE5 were consistent with the conformational requirements of them to MFA and with the crystal conformation of two representatives...
  41. ncbi Adult bone-marrow-derived mesenchymal stem cells contribute to wound healing of skin appendages
    Haihong Li
    Wound Healing and Cell Biology Laboratory, Burns Institute, The First Affiliated Hospital 304th Hospital, General Hospital of PLA, Trauma Center of Postgraduate Medical College, Beijing, People s Republic of China
    Cell Tissue Res 326:725-36. 2006
    ..We conclude that adult MSCs have the capacity to dock at specific sites, to contribute to wound healing of skin appendages, and to home toward marrow, and that engraftment of bone-marrow-derived cells is a functional event...
  42. ncbi Rapid assembly of matrix metalloprotease inhibitors using click chemistry
    Jun Wang
    Department of Chemistry, Medicinal Chemistry Program of the Office of Life Sciences, National University of Singapore, 3 Science Drive 3, Singapore 117543, Republic of Singapore
    Org Lett 8:3821-4. 2006
    ..The relative ease and convenience of the strategy in constructing focused chemical libraries for rapid in situ screening of MMPs is thereby demonstrated...
  43. ncbi Phase behavior of cationic hydroxyethyl cellulose-sodium dodecyl sulfate mixtures: effects of molecular weight and ethylene oxide side chain length of polymers
    Shuiqin Zhou
    Department of Chemistry, College of Staten Island, The City University of New York, 2800 Victory Boulevard, 10314, USA
    Langmuir 20:8482-9. 2004
    ..The effects of molecular weight and EO side chain length of polymers on the phase boundary, viscosity, and structure of cationic HEC-SDS complexes were discussed...
  44. ncbi Thermodynamic stability and kinetic foldability of a lattice protein model
    Jie Li
    National Lab of Solid State Microstructure and Physics Department, Nanjing University, Nanjing 210093, China
    J Chem Phys 120:6274-87. 2004
    ..The folding of model proteins with the MMJ potentials is fast. However, the relationship between the foldability and the thermodynamic stability of the mutated sequences is complex...
  45. ncbi Fumigaclavine C improves concanavalin A-induced liver injury in mice mainly via inhibiting TNF-alpha production and lymphocyte adhesion to extracellular matrices
    Ying Zhao
    State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China
    J Pharm Pharmacol 56:775-82. 2004
    ....
  46. ncbi Poly(phosphoester) ionomers as tissue-engineering scaffolds
    Andrew C A Wan
    Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
    J Biomed Mater Res B Appl Biomater 70:91-102. 2004
    ..These ionomers represent a novel class of biomaterials with readily controllable physical and chemical attributes for tissue engineering...
  47. ncbi Heparin-binding EGF-like growth factor regulates human extravillous cytotrophoblast development during conversion to the invasive phenotype
    Richard E Leach
    Department of Obstetrics and Gynecology, CS Mott Center for Human Growth and Development, Wayne State University School of Medicine, Detroit, MI 48201 1415, USA
    Dev Biol 266:223-37. 2004
    ..We conclude that HER-mediated autocrine and paracrine signaling by HB-EGF or other EGF family members induces cytotrophoblast differentiation to an invasive phenotype...
  48. pmc Photolysis of postsynaptic caged Ca2+ can potentiate and depress mossy fiber synaptic responses in rat hippocampal CA3 pyramidal neurons
    Jun Wang
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
    J Neurophysiol 91:1596-607. 2004
    ..Our results are consistent with a postsynaptic locus for the induction of at least some forms of synaptic plasticity at mossy fiber synapses...
  49. ncbi Role of 4-1BB in allograft rejection mediated by CD8+ T cells
    Jun Wang
    Emory Transplant Center and the Department of Surgery, Emory University School of Medicine, Atlanta, GA, USA
    Am J Transplant 3:543-51. 2003
    ..Our data suggest that in addition to providing a direct costimulatory signal to T cells, the 4-1BB pathway may regulate other important steps in the immune response such as the migration of T cells and dendritic cells...
  50. ncbi Overexpression and purification of recombinant atrial natriuretic peptide using hybrid fusion protein REF-ANP in Escherichia coli
    Jun Wang
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, PR China
    Protein Expr Purif 28:49-56. 2003
    ..Moreover, it is possible that the refolding and purification method along with the overexpression system described in this article may offer new ideas on optimizing expression and purification of other kinds of short peptides...
  51. ncbi Congruent MRI and near-infrared spectroscopy for functional and structural imaging of tumors
    Gultekin Gulsen
    John Tu and Thomas Yuen Center for Functional Onco Imaging, University of California Irvine, Irvine, California 92697, USA
    Technol Cancer Res Treat 1:497-505. 2002
    ....
  52. ncbi [Study on hydrolysis conditions of Dioscorea nipponica with uniform design method]
    Jun Wang
    Jiangsu University, Zhenjiang 212013
    Zhong Yao Cai 25:659-60. 2002
    ..To optimize hydrolysis conditions of Dioscorea nipponica so as to provide parameters for extracting diosgenin in industry...
  53. ncbi New polyphosphoramidate with a spermidine side chain as a gene carrier
    Jun Wang
    Johns Hopkins Singapore Biomedical Centre, Tissue and Therapeutic Engineering Laboratory, 10 Medical Drive, Singapore 117597, Singapore
    J Control Release 83:157-68. 2002
    ..In a non-invasive CNS gene delivery model, PPA-SP/DNA complexes yielded comparable bcl-2 expression as PEI/DNA complexes in mouse brain stem following injection of the complexes in the tongue...
  54. ncbi Arsenic trioxide, retinoic acid and Ara-c regulated the expression of annexin II on the surface of APL cells, a novel co-receptor for plasminogen/tissue plasminogen activator
    Xiaohui Zhang
    Department of Hematology, Union Hospital of Tongji Medical College of Huazhong University of Science and Technology, Wuhan, PR China
    Thromb Res 106:63-70. 2002
    ..Annexin II expression of APL cells may be downregulated by ATRA and As(2)O(3.) Therefore, both agents improve hyperfibrinolysis-related hemorrhage of APL, which induced APL cells to difference and apoptosis, respectively...
  55. ncbi Role of the N- and C-terminal regions of the PufX protein in the structural organization of the photosynthetic core complex of Rhodobacter sphaeroides
    Francesco Francia
    Department of Membrane Biochemistry Max Planck Institute for Biochemistry, Martinsried, Germany
    Eur J Biochem 269:1877-85. 2002
    ....
  56. ncbi Enantioselective synthesis of marine indole alkaloid hamacanthin B
    Biao Jiang
    The State Key Laboratory of Organometallic Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 354 Fenglin Road, Shanghai 200032, Peoples Republic of China
    J Org Chem 67:1396-8. 2002
    ..The stereochemistry of naturally isolated hamacanthin B is revealed as the (S)-configuration...
  57. ncbi [Antidepressant amides from Piper laetispicum C. DC]
    Sheng li Pan
    School of Pharmacy, Fudan University, Shanghai 200032, China
    Yao Xue Xue Bao 40:355-7. 2005
    ..To investigate the antidepressant constituents of Piper laetispicum C. DC...
  58. pmc Cleavage targets and the D-arginine-based inhibitors of the West Nile virus NS3 processing proteinase
    Sergey A Shiryaev
    The Burnham Institute, La Jolla, CA 92037, USA
    Biochem J 393:503-11. 2006
    ..Overall, our findings represent a foundation for in-depth mechanistic and structural studies as well as for the design of novel and efficient inhibitors of WNV NS3...
  59. ncbi Cellular prion protein promotes invasion and metastasis of gastric cancer
    Yanglin Pan
    State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, 17 Changle Western Rd, Xi an, Shaanxi Province, 710032, PR China
    FASEB J 20:1886-8. 2006
    ....
  60. pmc The antimalarial artemisinin synergizes with antibiotics to protect against lethal live Escherichia coli challenge by decreasing proinflammatory cytokine release
    Jun Wang
    Department of Pharmacology, College of Medicine, The Third Military Medical University, Gaotanyan Street 30, Shapingba District, Chongqing 400038, People s Republic of China
    Antimicrob Agents Chemother 50:2420-7. 2006
    ..ART treatment did, however, block the NF-kappaB activation induced by CpG ODN, LPS, or heat-killed E. coli. These findings provide compelling evidence that ART may be an important potential drug for sepsis treatment...
  61. ncbi Recent developments in microarray-based enzyme assays: from functional annotation to substrate/inhibitor fingerprinting
    Hongyan Sun
    Department of Chemistry, National University of Singapore, 3 Science Drive 3, 117543, Singapore, Singapore
    Anal Bioanal Chem 386:416-26. 2006
    ..Herein, we review recent developments in the fabrication of various types of microarray technologies (protein-, peptide- and small-molecule-based microarrays) and their applications in high-throughput characterizations of enzymes...
  62. ncbi The expression of monocyte chemoattractant protein-1 and C-C chemokine receptor 2 in post-kidney transplant patients and the influence of simvastatin treatment
    Jun Wang
    Department of Nephrology, The Fifth Affiliated Hospital of Sun Yat sen University, 52 Meihua Dong Road, Zhuhai 519000, Guangdong, PR China
    Clin Chim Acta 373:44-8. 2006
    ..Inflammation is involved in the pathogenesis of chronic allograft nephropathy (CAN) and cardiovascular disease (CVD) in post-kidney transplantation...
  63. ncbi Functional analysis of the purified anandamide-generating phospholipase D as a member of the metallo-beta-lactamase family
    Jun Wang
    Department of Biochemistry, School of Medicine, Kagawa University, Miki, Japan
    J Biol Chem 281:12325-35. 2006
    ..Thus, NAPE-PLD appeared to function through a mechanism similar to those of the well characterized members of this family but play a unique role in the lipid metabolism of animal tissues...
  64. ncbi Regulation of the preprotachykinin-I gene promoter through a protein kinase A-dependent, cyclic AMP response element-binding protein-independent mechanism
    Irina E Calin-Jageman
    Department of Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Neurochem 97:255-64. 2006
    ..We conclude that, at least in RINm5F cells, cAMP-mediated up-regulation of PPT gene expression does not involve CREB or CREB-related transcription factor recruitment to the AP1/CRE site...
  65. ncbi J1 acylase, a glutaryl-7-aminocephalosporanic acid acylase from Bacillus laterosporus J1, is a member of the alpha/beta-hydrolase fold superfamily
    Ming Hon Yau
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China
    FEBS Lett 580:1465-71. 2006
    ..Mutations to alanine at these positions were found to completely inactivate the enzyme. These results suggest that J1 acylase is a member of the alpha/beta-hydrolase fold superfamily with a serine-histidine-aspartate catalytic triad...
  66. ncbi Folding with downhill behavior and low cooperativity of proteins
    Guanghong Zuo
    Department of Physics, Nanjing University, China
    Proteins 63:165-73. 2006
    ..These could enable us to identify the downhill folding or the cooperative two-state folding behavior solely from the native structures of proteins...
  67. ncbi Sonocatalytic degradation of methyl parathion in the presence of nanometer and ordinary anatase titanium dioxide catalysts and comparison of their sonocatalytic abilities
    Jun Wang
    Department of Chemistry, Liaoning University, Shenyang, PR China
    Ultrason Sonochem 13:493-500. 2006
    ..The kinetics for the degradation process of methyl parathion follows the first-order reaction. The degradation ratio of methyl parathion surpassed 90% within 50min in the optimal experiment conditions...
  68. ncbi Capillary array electrophoresis for the research of asymmetric transformation reaction of L-proline to D-proline
    Jun Wang
    State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P R China
    J Sep Sci 28:2551-4. 2005
    ....
  69. ncbi Study on the influence of potassium iodate on the metabolism of Escherichia coli by intrinsic fluorescence
    Hongmei Wang
    Department of Chemistry, Wuhan University, Wuhan 430072, China
    Spectrochim Acta A Mol Biomol Spectrosc 64:316-20. 2006
    ..And the bacteria could not continue growing if the KIO3 was over 6.67 mmol/L.We could conclude that potassium iodate has great inhibiting effects on the growth of E. coli through the pathway of protein synthesis and respiratory chain...
  70. ncbi C-terminal PAL motif of presenilin and presenilin homologues required for normal active site conformation
    Jun Wang
    Department of Psychiatry, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Neurochem 96:218-27. 2006
    ..Together, these observations strongly support the hypothesis that the PAL motif contributes to the active site conformation of gamma-secretase and of SPP...
  71. ncbi Folding behavior of chaperonin-mediated substrate protein
    Wei Xin Xu
    National Laboratory of Solid State Microstructure, Department of Physics, Nanjing University, Nanjing, China
    Proteins 61:777-94. 2005
    ....
  72. ncbi Catalytic antibody therapy against the insecticide carbaryl
    Jun Wang
    Department of Immunology, University of Texas M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030, USA
    Biochem Biophys Res Commun 291:605-10. 2002
    ..Our results suggest that polyclonal catalytic antibodies can be used as therapeutic reagents in vivo...