N A Thornberry

Summary

Affiliation: Merck Research Laboratories
Country: USA

Publications

  1. ncbi request reprint The three-dimensional structure of apopain/CPP32, a key mediator of apoptosis
    J Rotonda
    Department of Biochemistry, Merck Research Laboratories, Rahway, New Jersey 07065 0900, USA
    Nat Struct Biol 3:619-25. 1996
  2. ncbi request reprint A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis
    N A Thornberry
    Department of Enzymology, Merck Research Laboratories, Rahway, New Jersey 07065, USA
    J Biol Chem 272:17907-11. 1997
  3. ncbi request reprint Caspases: key mediators of apoptosis
    N A Thornberry
    Merck Research Laboratories, Rahway, NJ 07065, USA
    Chem Biol 5:R97-103. 1998
  4. ncbi request reprint Purification and catalytic properties of human caspase family members
    M Garcia-Calvo
    Department of Enzymology, Merck Research Laboratories, R80W 250, P O Box 2000, Rahway, New Jersey 07065, USA
    Cell Death Differ 6:362-9. 1999
  5. ncbi request reprint The three-dimensional structure of human granzyme B compared to caspase-3, key mediators of cell death with cleavage specificity for aspartic acid in P1
    J Rotonda
    Department of Endocrinology and Chemical Biology, Merck Research Laboratories, Rahway, NJ 07065 0900, USA
    Chem Biol 8:357-68. 2001
  6. ncbi request reprint Inhibition of human caspases by peptide-based and macromolecular inhibitors
    M Garcia-Calvo
    Department of Enzymology, Merck Research Laboratories, Rahway, New Jersey 07065, USA
    J Biol Chem 273:32608-13. 1998
  7. ncbi request reprint A combinatorial approach for determining protease specificities: application to interleukin-1beta converting enzyme (ICE)
    T A Rano
    Department of Molecular Design and Diversity, Merck Research Laboratories, R123 232, PO Box 2000, Rahway, New Jersey 07065, USA
    Chem Biol 4:149-55. 1997
  8. ncbi request reprint A novel heterodimeric cysteine protease is required for interleukin-1 beta processing in monocytes
    N A Thornberry
    Department of Biochemistry, Merck Research Laboratories, Rahway, New Jersey 07065
    Nature 356:768-74. 1992
  9. ncbi request reprint Caspases cleave focal adhesion kinase during apoptosis to generate a FRNK-like polypeptide
    F G Gervais
    Department of Biochemistry and Molecular Biology, Merck Frosst Centre for Therapeutic Research, Pointe Claire Dorval, Quebec H9R 4P8, Canada
    J Biol Chem 273:17102-8. 1998
  10. ncbi request reprint c-IAP1 is cleaved by caspases to produce a proapoptotic C-terminal fragment
    R J Clem
    Department of Molecular Microbiology and Immunology, Johns Hopkins Schools of Public Health and Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 276:7602-8. 2001

Collaborators

  • D W Nicholson
  • M Garcia-Calvo
  • J Rotonda
  • J W Becker
  • S Roy
  • D K Miller
  • A D Howard
  • A Ali
  • J Yuan
  • Yuan Zhu
  • ROLLIE CLEM
  • Barbara Leiting
  • F G Gervais
  • E P Peterson
  • J P Vaillancourt
  • D M Rasper
  • F Andrade
  • A Rosen
  • L A Casciola-Rosen
  • Z Song
  • M Mancini
  • S Xanthoudakis
  • J Huang
  • S C Ruffolo
  • M R Hayden
  • T A Rano
  • Y P Goldberg
  • E S Alnemri
  • H G Bull
  • G W Ketner
  • B Guan
  • A Bergman
  • C E Machamer
  • H Steller
  • R J Zamboni
  • D Xu
  • G S Robertson
  • E E Clarke
  • H Zheng
  • M Rigby
  • A Leblanc
  • L H Van der Ploeg
  • D Smith
  • M S Shearman
  • F Hornung
  • D Martindale
  • M J Lenardo
  • V M Houtzager
  • S C Black
  • B F Koop
  • P Tawa
  • I Seiden
  • D P MacPherson
  • J Nasir
  • S L Keen
  • S Hadano
  • T Timkey
  • K T Chapman
  • R K Graham
  • D J Livingston
  • M Bromm
  • P Kazemi-Esfarjani
  • M A Kalchman
  • W W Wong
  • H B Koide
  • G Salvesen

Detail Information

Publications19

  1. ncbi request reprint The three-dimensional structure of apopain/CPP32, a key mediator of apoptosis
    J Rotonda
    Department of Biochemistry, Merck Research Laboratories, Rahway, New Jersey 07065 0900, USA
    Nat Struct Biol 3:619-25. 1996
    ....
  2. ncbi request reprint A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis
    N A Thornberry
    Department of Enzymology, Merck Research Laboratories, Rahway, New Jersey 07065, USA
    J Biol Chem 272:17907-11. 1997
    ....
  3. ncbi request reprint Caspases: key mediators of apoptosis
    N A Thornberry
    Merck Research Laboratories, Rahway, NJ 07065, USA
    Chem Biol 5:R97-103. 1998
    ..Insights into their biological roles, structure and mechanism are enabling investigators to begin to explore the therapeutic potential of caspase inhibition...
  4. ncbi request reprint Purification and catalytic properties of human caspase family members
    M Garcia-Calvo
    Department of Enzymology, Merck Research Laboratories, R80W 250, P O Box 2000, Rahway, New Jersey 07065, USA
    Cell Death Differ 6:362-9. 1999
    ..g. pH, NaCl, Ca2+) on the activities of these enzymes. Some of these variables have a profound effect on the rate of catalysis, a finding that may have important biological implications...
  5. ncbi request reprint The three-dimensional structure of human granzyme B compared to caspase-3, key mediators of cell death with cleavage specificity for aspartic acid in P1
    J Rotonda
    Department of Endocrinology and Chemical Biology, Merck Research Laboratories, Rahway, NJ 07065 0900, USA
    Chem Biol 8:357-68. 2001
    ....
  6. ncbi request reprint Inhibition of human caspases by peptide-based and macromolecular inhibitors
    M Garcia-Calvo
    Department of Enzymology, Merck Research Laboratories, Rahway, New Jersey 07065, USA
    J Biol Chem 273:32608-13. 1998
    ....
  7. ncbi request reprint A combinatorial approach for determining protease specificities: application to interleukin-1beta converting enzyme (ICE)
    T A Rano
    Department of Molecular Design and Diversity, Merck Research Laboratories, R123 232, PO Box 2000, Rahway, New Jersey 07065, USA
    Chem Biol 4:149-55. 1997
    ..It was the first member of a new cysteine protease family to be identified. Members of this family have functions in both inflammation and apoptosis...
  8. ncbi request reprint A novel heterodimeric cysteine protease is required for interleukin-1 beta processing in monocytes
    N A Thornberry
    Department of Biochemistry, Merck Research Laboratories, Rahway, New Jersey 07065
    Nature 356:768-74. 1992
    ..Selective inhibition of the enzyme in human blood monocytes blocks production of mature IL-1 beta, indicating that it is a potential therapeutic target...
  9. ncbi request reprint Caspases cleave focal adhesion kinase during apoptosis to generate a FRNK-like polypeptide
    F G Gervais
    Department of Biochemistry and Molecular Biology, Merck Frosst Centre for Therapeutic Research, Pointe Claire Dorval, Quebec H9R 4P8, Canada
    J Biol Chem 273:17102-8. 1998
    ..This appears to be the first example of a caspase substrate where the cleavage sites are not conserved between species...
  10. ncbi request reprint c-IAP1 is cleaved by caspases to produce a proapoptotic C-terminal fragment
    R J Clem
    Department of Molecular Microbiology and Immunology, Johns Hopkins Schools of Public Health and Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 276:7602-8. 2001
    ..To the contrary, deletion of the caspase recruitment domain increased proapoptotic activity, apparently by stabilizing the C-terminal fragment...
  11. ncbi request reprint Adenovirus L4-100K assembly protein is a granzyme B substrate that potently inhibits granzyme B-mediated cell death
    F Andrade
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Immunity 14:751-61. 2001
    ..It represents a novel class of viral protease inhibitor, in which an essential, multifunctional viral protein, which is vulnerable to specific proteolysis by GrB, expresses inhibitory function against that protease...
  12. ncbi request reprint Involvement of caspases in proteolytic cleavage of Alzheimer's amyloid-beta precursor protein and amyloidogenic A beta peptide formation
    F G Gervais
    Department of Pharmacology, Biochemistry, and Molecular Biology, Merck Frosst Centre for Therapeutic Research, Merck Research Laboratories, Kirkland, Quebec, Canada
    Cell 97:395-406. 1999
    ..Caspases thus appear to play a dual role in proteolytic processing of APP and the resulting propensity for A beta peptide formation, as well as in the ultimate apoptotic death of neurons in Alzheimer's disease...
  13. ncbi request reprint Cleavage of huntingtin by apopain, a proapoptotic cysteine protease, is modulated by the polyglutamine tract
    Y P Goldberg
    Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada
    Nat Genet 13:442-9. 1996
    ..Our results show that huntingtin is cleaved by cysteine proteases and suggest that HD might be a disorder of inappropriate apoptosis...
  14. ncbi request reprint Cell death attenuation by 'Usurpin', a mammalian DED-caspase homologue that precludes caspase-8 recruitment and activation by the CD-95 (Fas, APO-1) receptor complex
    D M Rasper
    Department of Biochemistry and Molecular Biology, Merck Frosst Centre for Therapeutic Research, Pointe Claire Dorval, Quebec, Canada, H9R 4P8
    Cell Death Differ 5:271-88. 1998
    ..Usurpin thus appears to be an endogenous modulator of apoptosis sensitivity in mammalian cells, including the susceptibility of cardiac myocytes to apoptotic death following ischemia/ reperfusion injury...
  15. pmc Caspase-2 is localized at the Golgi complex and cleaves golgin-160 during apoptosis
    M Mancini
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Cell Biol 149:603-12. 2000
    ..We propose that the Golgi complex, like mitochondria, senses and integrates unique local conditions, and transduces pro-apoptotic signals through local caspases, which regulate local effectors...
  16. ncbi request reprint Human ICE/CED-3 protease nomenclature
    E S Alnemri
    Cell 87:171. 1996
  17. ncbi request reprint Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis
    D W Nicholson
    Department of Biochemistry and Molecular Biology, Merck Frosst Centre for Therapeutic Research, Pointe Claire Dorval, Quebec, Canada
    Nature 376:37-43. 1995
    ..A potent peptide aldehyde inhibitor has been developed and shown to prevent apoptotic events in vitro, suggesting that apopain/CPP32 is important for the initiation of apoptotic cell death...
  18. pmc Maintenance of caspase-3 proenzyme dormancy by an intrinsic "safety catch" regulatory tripeptide
    S Roy
    Department of Biochemistry and Molecular Biology, Merck Frosst Centre for Therapeutic Research, Kirkland, Quebec, PQ Canada
    Proc Natl Acad Sci U S A 98:6132-7. 2001
    ..We propose that the caspase-3 safety catch is a key regulatory checkpoint in the apoptotic cascade that regulates terminal events in the caspase cascade by modulating the triggering of caspase-3 activation...
  19. pmc Biochemical and genetic interactions between Drosophila caspases and the proapoptotic genes rpr, hid, and grim
    Z Song
    Departments of Biology and Brain and Cognitive Sciences, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Mol Cell Biol 20:2907-14. 2000
    ..Significantly, GMR-rpr and GMR-grim, but not GMR-hid, dramatically enhanced the eye phenotype of GMR-fl-dcp-1 flies. These results indicate that Reaper and Grim, but not HID, can activate DCP-1 in vivo...