Petra Neddermann

Summary

Affiliation: Merck Research Laboratories
Country: USA

Publications

  1. pmc Reduction of hepatitis C virus NS5A hyperphosphorylation by selective inhibition of cellular kinases activates viral RNA replication in cell culture
    Petra Neddermann
    IRBM, Via Pontina Km 30, 600, 00040 Pomezia, Rome, Italy
    J Virol 78:13306-14. 2004
  2. pmc A novel, inducible, eukaryotic gene expression system based on the quorum-sensing transcription factor TraR
    Petra Neddermann
    Istituto di Ricerche di Biologia Molecolare P Angeletti, Via Pontina Km 30 600, 00040 Pomezia Roma, Italy
    EMBO Rep 4:159-65. 2003
  3. pmc The crystal structure of the quorum sensing protein TraR bound to its autoinducer and target DNA
    Alessandro Vannini
    IRBM P Angeletti, Via Pontina Km 30 600, I 00040 Pomezia, Rome, Italy
    EMBO J 21:4393-401. 2002
  4. pmc Structural and functional analysis of the human HDAC4 catalytic domain reveals a regulatory structural zinc-binding domain
    Matthew J Bottomley
    Istituto di Ricerche di Biologia Molecolare P Angeletti, Merck Research Laboratories, Via Pontina Km 30 600, 00040 Pomezia Roma, Italy
    J Biol Chem 283:26694-704. 2008
  5. ncbi request reprint Hepatitis C virus NS5A is a direct substrate of casein kinase I-alpha, a cellular kinase identified by inhibitor affinity chromatography using specific NS5A hyperphosphorylation inhibitors
    Manuela Quintavalle
    Istituto di Ricerche di Biologia Molecolare P Angeletti, Via Pontina Km 30 6, 00040 Pomezia, Rome, Italy
    J Biol Chem 282:5536-44. 2007
  6. ncbi request reprint Synthesis and SAR of piperazinyl-N-phenylbenzamides as inhibitors of hepatitis C virus RNA replication in cell culture
    Immacolata Conte
    Department of Medicinal Chemistry, Istituto di Ricerche di Biologia Molecolare P Angeletti S p A, Merck Research Laboratories Rome, 00040 Pomezia, Rome, Italy
    Bioorg Med Chem Lett 19:1779-83. 2009
  7. ncbi request reprint Determination of the stoichiometry of noncovalent complexes using reverse-phase high-performance liquid chromatography coupled with electrospray ion trap mass spectrometry
    Laura Orsatti
    Department of Pharmacology, Istituto di Ricerche di Biologia Molecolare P Angeletti, IRBM, Via Pontina Km 30 600, Pomezia Rome, Italy
    Anal Biochem 309:11-8. 2002
  8. pmc The alpha isoform of protein kinase CKI is responsible for hepatitis C virus NS5A hyperphosphorylation
    Manuela Quintavalle
    Istituto di Ricerche di Biologia Molecolare P Angeletti, 00040 Pomezia Roma, Italy
    J Virol 80:11305-12. 2006
  9. doi request reprint NS5A phosphorylation and hyperphosphorylation
    Petra Neddermann
    Istituto di Ricerche di Biologia Molecolare P Angeletti, Pomezia Rome, Italy
    Methods Mol Biol 510:95-110. 2009
  10. ncbi request reprint Crystallization and preliminary X-ray diffraction studies of the transcriptional regulator TraR bound to its cofactor and to a specific DNA sequence
    Alessandro Vannini
    Department of Biochemistry, IRBM P Angeletti, 00040 Pomezia, Rome, Italy
    Acta Crystallogr D Biol Crystallogr 58:1362-4. 2002

Collaborators

Detail Information

Publications13

  1. pmc Reduction of hepatitis C virus NS5A hyperphosphorylation by selective inhibition of cellular kinases activates viral RNA replication in cell culture
    Petra Neddermann
    IRBM, Via Pontina Km 30, 600, 00040 Pomezia, Rome, Italy
    J Virol 78:13306-14. 2004
    ..This is the first direct demonstration that the presence of high amounts of NS5A-p58 causes inhibition of HCV RNA replication in cell culture and that this inhibition can be relieved by kinase inhibitors...
  2. pmc A novel, inducible, eukaryotic gene expression system based on the quorum-sensing transcription factor TraR
    Petra Neddermann
    Istituto di Ricerche di Biologia Molecolare P Angeletti, Via Pontina Km 30 600, 00040 Pomezia Roma, Italy
    EMBO Rep 4:159-65. 2003
    ..The basal level of transcription was low in the absence of 3-oxo-C(8)-HSL, and gene expression was stimulated up to 1,000-fold at a saturating concentration of 3-oxo-C(8)-HSL...
  3. pmc The crystal structure of the quorum sensing protein TraR bound to its autoinducer and target DNA
    Alessandro Vannini
    IRBM P Angeletti, Via Pontina Km 30 600, I 00040 Pomezia, Rome, Italy
    EMBO J 21:4393-401. 2002
    ..In TraR, the gene fusion is between a GAF/PAS domain and a DNA-binding domain, resulting in a specific transcriptional regulator involved in quorum sensing...
  4. pmc Structural and functional analysis of the human HDAC4 catalytic domain reveals a regulatory structural zinc-binding domain
    Matthew J Bottomley
    Istituto di Ricerche di Biologia Molecolare P Angeletti, Merck Research Laboratories, Via Pontina Km 30 600, 00040 Pomezia Roma, Italy
    J Biol Chem 283:26694-704. 2008
    ..HDAC3 repressor complex. Together, these data suggest a key role of the structural zinc-binding domain in the regulation of class IIa HDAC functions...
  5. ncbi request reprint Hepatitis C virus NS5A is a direct substrate of casein kinase I-alpha, a cellular kinase identified by inhibitor affinity chromatography using specific NS5A hyperphosphorylation inhibitors
    Manuela Quintavalle
    Istituto di Ricerche di Biologia Molecolare P Angeletti, Via Pontina Km 30 6, 00040 Pomezia, Rome, Italy
    J Biol Chem 282:5536-44. 2007
    ..In vitro kinase reactions performed with NS5A peptides show that Ser-2204 is a preferred substrate residue for CKI-alpha after pre-phosphorylation of Ser-2201...
  6. ncbi request reprint Synthesis and SAR of piperazinyl-N-phenylbenzamides as inhibitors of hepatitis C virus RNA replication in cell culture
    Immacolata Conte
    Department of Medicinal Chemistry, Istituto di Ricerche di Biologia Molecolare P Angeletti S p A, Merck Research Laboratories Rome, 00040 Pomezia, Rome, Italy
    Bioorg Med Chem Lett 19:1779-83. 2009
    ..We report here substituted piperazinyl-N-(aryl)benzamides as potent inhibitors of HCV replication exerted via modulation of the dimerization of NS5A...
  7. ncbi request reprint Determination of the stoichiometry of noncovalent complexes using reverse-phase high-performance liquid chromatography coupled with electrospray ion trap mass spectrometry
    Laura Orsatti
    Department of Pharmacology, Istituto di Ricerche di Biologia Molecolare P Angeletti, IRBM, Via Pontina Km 30 600, Pomezia Rome, Italy
    Anal Biochem 309:11-8. 2002
    ..Both TraR/AAI and NS3/NS4A complexes have 1:1 stoichiometries, in line with those already reported in the literature...
  8. pmc The alpha isoform of protein kinase CKI is responsible for hepatitis C virus NS5A hyperphosphorylation
    Manuela Quintavalle
    Istituto di Ricerche di Biologia Molecolare P Angeletti, 00040 Pomezia Roma, Italy
    J Virol 80:11305-12. 2006
    ..Finally, we showed that down-regulation of CKI-alpha attenuates HCV RNA replication...
  9. doi request reprint NS5A phosphorylation and hyperphosphorylation
    Petra Neddermann
    Istituto di Ricerche di Biologia Molecolare P Angeletti, Pomezia Rome, Italy
    Methods Mol Biol 510:95-110. 2009
    ..The problem with this approach is that purified components, like the purified NS5A substrate and the kinase of interest, are not always available...
  10. ncbi request reprint Crystallization and preliminary X-ray diffraction studies of the transcriptional regulator TraR bound to its cofactor and to a specific DNA sequence
    Alessandro Vannini
    Department of Biochemistry, IRBM P Angeletti, 00040 Pomezia, Rome, Italy
    Acta Crystallogr D Biol Crystallogr 58:1362-4. 2002
    ..A three-wavelength MAD data set for the seleno-L-methionine-substituted form has been collected to a resolution of 3 A. 20 of the 24 crystallographically independent selenium sites were located as part of the MAD-phasing process...
  11. doi request reprint Probing the elusive catalytic activity of vertebrate class IIa histone deacetylases
    Philip Jones
    IRBM Merck Research Laboratories, Via Pontina Km 30, 600, 00040 Pomezia, Italy
    Bioorg Med Chem Lett 18:1814-9. 2008
    ..The low efficiency can be restored either by: mutation of an active site histidine to tyrosine, or by the use of a non-acetylated lysine substrate, allowing the development of assays to identify class IIa HDAC inhibitors...
  12. pmc High-throughput screening of the yeast kinome: identification of human serine/threonine protein kinases that phosphorylate the hepatitis C virus NS5A protein
    Carlos Coito
    Department of Microbiology, Washington National Primate Research Center, University of Washington, Seattle, Washington 98195, USA
    J Virol 78:3502-13. 2004
    ..Our results suggest that this novel approach may be generally applicable to the screening of other protein biochemical activities by mechanistic class...
  13. pmc Subversion of cell signaling pathways by hepatitis C virus nonstructural 5A protein via interaction with Grb2 and P85 phosphatidylinositol 3-kinase
    Yupeng He
    Department of Microbiology, School of Medicine, University of Washington, Seattle, Washington 98195, USA
    J Virol 76:9207-17. 2002
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