Lee P Lim
Affiliation: Merck Research Laboratories
- Prediction of plant microRNA targetsMatthew W Rhoades
Whitehead Institute for Biomedical Research, 9 Cambridge Center, MA 02142, USA
Cell 110:513-20. 2002..The targeting of developmental transcription factors suggests that many plant miRNAs function during cellular differentiation to clear key regulatory transcripts from daughter cell lineages...
- Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAsLee P Lim
Rosetta Inpharmatics, Merck and Co, 401 Terry Avenue N, Seattle, Washington 98109, USA
Nature 433:769-73. 2005..Moreover, miR-1 and miR-124, and presumably other tissue-specific miRNAs, seem to downregulate a far greater number of targets than previously appreciated, thereby helping to define tissue-specific gene expression in humans...
- Mustering the micromanagersLee P Lim
Nat Biotechnol 25:996-7. 2007
- microRNAs regulate human embryonic stem cell divisionJunlin Qi
Department of Biochemistry and Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA
Cell Cycle 8:3729-41. 2009..These data support a model for hESC cell cycle control in which miRNAs regulate negative cell cycle modulators at two phases of the cell cycle to ensure proper replenishment of the stem cell population...
- Global survey of genomic imprinting by transcriptome sequencingTomas Babak
Rosetta Inpharmatics, LLC, a wholly owned subsidiary of Merck and Co, 401 Terry Avenue North, Seattle, WA 98109, USA
Curr Biol 18:1735-41. 2008..Such an approach will allow researchers to study imprinting without restricting themselves to individual loci or specific transcripts...
- Simple, quantitative primer-extension PCR assay for direct monitoring of microRNAs and short-interfering RNAsChristopher K Raymond
Rosetta Inpharmatics, Seattle, WA 98109, USA
RNA 11:1737-44. 2005..We used the method for quantitative analysis of six microRNAs across 12 tissue samples. The data confirm striking variation in the patterns of expression of these noncoding regulatory RNAs...
- MicroRNA targeting specificity in mammals: determinants beyond seed pairingAndrew Grimson
Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Mol Cell 27:91-105. 2007..Because it predicts site efficacy without recourse to evolutionary conservation, the model also identifies effective nonconserved sites and siRNA off-targets...
- The widespread impact of mammalian MicroRNAs on mRNA repression and evolutionKyle Kai-How Farh
Whitehead Institute for Biomedical Research, Department of Biology, Massachusetts Institute of Technology, and Howard Hughes Medical Institute, 9 Cambridge Center, Cambridge, MA 02142, USA
Science 310:1817-21. 2005....
- Patterns of flanking sequence conservation and a characteristic upstream motif for microRNA gene identificationUwe Ohler
Department of Biology, Massachusetts Institute of Technology, Cambridge 02142, USA
RNA 10:1309-22. 2004..The improved analysis supports our previous assertion that miRNA gene identification is nearing completion in C. elegans with apparently no more than 20 miRNA genes now remaining to be identified...
- A microRNA component of the p53 tumour suppressor networkLin He
Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
Nature 447:1130-4. 2007..The p53 network suppresses tumour formation through the coordinated activation of multiple transcriptional targets, and miR-34 may act in concert with other effectors to inhibit inappropriate cell proliferation...
- The microRNAs of Caenorhabditis elegansLee P Lim
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
Genes Dev 17:991-1008. 2003..Our census of the worm miRNAs and their expression patterns helps define this class of noncoding RNAs, lays the groundwork for functional studies, and provides the tools for more comprehensive analyses of miRNA genes in other species...
- Vertebrate microRNA genesLee P Lim
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Science 299:1540. 2003