Research Topics
Genomes and Genes | Aimee L JacksonSummaryAffiliation: Merck Research Laboratories Country: USA Publications
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Publications
Expression profiling reveals off-target gene regulation by RNAiAimee L Jackson
Rosetta Inpharmatics LLC, 12040 115th Avenue NE, Kirkland, Washington 98034, USA
Nat Biotechnol 21:635-7. 2003..These results demonstrate that siRNAs may cross-react with targets of limited sequence similarity...- Noise amidst the silence: off-target effects of siRNAs?Aimee L Jackson
Rosetta Inpharmatics LLC, 401 Terry Avenue N, Seattle, WA 98109, USA
Trends Genet 20:521-4. 2004..In the meantime, caution is warranted in interpreting gene function and phenotypes resulting from RNAi experiments... 
Widespread siRNA "off-target" transcript silencing mediated by seed region sequence complementarityAimee L Jackson
Rosetta Inpharmatics, LLC, Seattle, WA 98109, USA
RNA 12:1179-87. 2006..Thus, short stretches of sequence complementarity to the siRNA or shRNA seed region are key to the silencing of unintended transcripts...- Position-specific chemical modification of siRNAs reduces "off-target" transcript silencingAimee L Jackson
Rosetta Inpharmatics, LLC, Seattle, WA 98109, USA
RNA 12:1197-205. 2006.... - MicroRNA-like off-target transcript regulation by siRNAs is species specificJulja Burchard
Rosetta Inpharmatics LLC, Seattle, Washington 98109, USA
RNA 15:308-15. 2009..Therefore, siRNA therapeutics may trigger microRNA-like silencing of many unintended targets in vivo, and the potential toxicities caused by these off-target gene regulations cannot be accurately assessed in rodent models... 
Coordinated regulation of cell cycle transcripts by p53-Inducible microRNAs, miR-192 and miR-215Sara A Georges
Rosetta Inpharmatics LLC, Seattle, Washington WA 98109, USA
Cancer Res 68:10105-12. 2008..Our results showing a role for miR-192/215 in cell proliferation combined with recent observations that these miRNAs are underexpressed in primary cancers support the idea that miR-192 and miR-215 function as tumor suppressors...- MicroRNAs in the miR-106b family regulate p21/CDKN1A and promote cell cycle progressionIrena Ivanovska
Rosetta Inpharmatics LLC, 401 Terry Ave N, Seattle, WA 98109, USA
Mol Cell Biol 28:2167-74. 2008..We also show that miR-106b overrides a doxorubicin-induced DNA damage checkpoint. Thus, miR-106b family members contribute to tumor cell proliferation in part by regulating cell cycle progression and by modulating checkpoint functions... - Transcripts targeted by the microRNA-16 family cooperatively regulate cell cycle progressionPeter S Linsley
Rosetta Inpharmatics, LLC, 401 Terry Ave N, Seattle, WA 98109, USA
Mol Cell Biol 27:2240-52. 2007..Simultaneous silencing of these genes was more effective at blocking cell cycle progression than disruption of the individual genes. Thus, miR-16 coordinately regulates targets that may act in concert to control cell cycle progression... - Small interfering RNA screens reveal enhanced cisplatin cytotoxicity in tumor cells having both BRCA network and TP53 disruptionsSteven R Bartz
Rosetta Inpharmatics, LLC, Seattle, WA 98109, USA
Mol Cell Biol 26:9377-86. 2006..Thus, tumor cells having disruptions in BRCA1/2 network genes and TP53 together are more sensitive to cisplatin than cells with either disruption alone... - Myc-regulated microRNAs attenuate embryonic stem cell differentiationChin Hsing Lin
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 4417, USA
EMBO J 28:3157-70. 2009..In contrast, knockdown of the endogenous miRNAs accelerate differentiation. Our data show that in ES cells c-Myc acts, in part, through a subset of miRNAs to attenuate differentiation... - How will RNAi facilitate drug development?Steven Bartz
Rosetta Inpharmatics, LLC, a wholly owned subsidiary of Merck and Co, Inc, 401 Terry Avenue North, Seattle, WA 98109, USA
Sci STKE 2005:pe39. 2005..Here, we discuss the potential impact of RNAi screens on target identification and validation and consider issues that warrant caution when interpreting RNAi screening results... - Genome-wide resources of endoribonuclease-prepared short interfering RNAs for specific loss-of-function studiesRalf Kittler
Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D 01307 Dresden, Germany
Nat Methods 4:337-44. 2007..Hence, the presented esiRNA libraries offer an efficient, cost-effective and specific alternative to presently available mammalian RNAi resources... - A microRNA component of the p53 tumour suppressor networkLin He
Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
Nature 447:1130-4. 2007..The p53 network suppresses tumour formation through the coordinated activation of multiple transcriptional targets, and miR-34 may act in concert with other effectors to inhibit inappropriate cell proliferation... - Genome-scale RNAi profiling of cell division in human tissue culture cellsRalf Kittler
Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D 01307 Dresden, Germany
Nat Cell Biol 9:1401-12. 2007..Our work provides an experimental framework from which the systematic analysis of novel genes necessary for cell division in human cells can begin... - Minimizing the risk of reporting false positives in large-scale RNAi screensChristophe J Echeverri
Cenix BioScience GmbH, Tatzberg 47, Dresden, 10307, Germany
Nat Methods 3:777-9. 2006....