Research Topics
Species | John C CastleSummaryAffiliation: Merck Research Laboratories Country: USA Publications
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Detail Information
Publications
Development of a microarray platform for FFPET profiling: application to the classification of human tumorsSven Duenwald
Translational Sciences, Department of Molecular Profiling, Merck Research Laboratories, Seattle, WA 98109, USA
J Transl Med 7:65. 2009..Methods are needed to profile these FFPET samples that are linked to clinical outcomes to generate hypotheses that could lead to classifiers for clinical applications...
A comprehensive transcript index of the human genome generated using microarrays and computational approachesEric E Schadt
Rosetta Inpharmatics LLC, 12040 115th Avenue NE, Kirkland, WA 98034, USA
Genome Biol 5:R73. 2004..Further, expression activity over at least six conditions was more generally assessed using genomic tiling arrays consisting of probes tiled through a repeat-masked version of the genomic sequence making up chromosomes 20 and 22...
Comparative expression pathway analysis of human and canine mammary tumorsPaolo Uva
IRBM P Angeletti, Merck MRL Rome, Pomezia, Italy
BMC Genomics 10:135. 2009....
Exon and junction microarrays detect widespread mouse strain- and sex-bias expression differencesWan Lin Su
Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98195, USA
BMC Genomics 9:273. 2008..Here, we surveyed the effect of strain and sex on liver gene and exon expression using male and female mice from three different inbred strains...
ACC2 is expressed at high levels in human white adipose and has an isoform with a novel N-terminus [corrected]John C Castle
Rosetta Inpharmatics LLC, Seattle, Washington, United States of America
PLoS ONE 4:e4369. 2009..The results demonstrate a significant difference in ACC expression between human and rodents, which may introduce difficulties for the use of rodent models for development of ACC inhibitors...
Definition, conservation and epigenetics of housekeeping and tissue-enriched genesXinwei She
Rosetta Inpharmatics LLC, Seattle, WA 98109, USA
BMC Genomics 10:269. 2009..The genomic features of these two groups of genes expressed in opposing patterns may shed light on the mechanisms by which cells maintain basic and tissue-specific functions...
Expression of 24,426 human alternative splicing events and predicted cis regulation in 48 tissues and cell linesJohn C Castle
Rosetta Inpharmatics LLC, Seattle, WA 98109, USA
Nat Genet 40:1416-25. 2008..UCUCU and UGCAUG seem to have similar function but independent action, occurring 5' and 3', respectively, of 33% of the cassette exons upregulated in skeletal muscle but co-occurring for only 2%...
NSR-seq transcriptional profiling enables identification of a gene signature of Plasmodium falciparum parasites infecting childrenMarissa Vignali
Seattle Biomedical Research Institute, Seattle, Washington, USA
J Clin Invest 121:1119-29. 2011..Our study provides proof of principle that NSR-seq represents an approach that can be used to study clinical isolates of parasites causing severe malaria syndromes as well other blood-borne pathogens and blood-related diseases...
DNA copy number, including telomeres and mitochondria, assayed using next-generation sequencingJohn C Castle
Rosetta Inpharmatics LLC, Merck and Co, Inc, Seattle, Washington 98109, USA
BMC Genomics 11:244. 2010..DNA copy number variations occur within populations and aberrations can cause disease. We sought to develop an improved lab-automatable, cost-efficient, accurate platform to profile DNA copy number...
SNPs occur in regions with less genomic sequence conservationJohn C Castle
Rosetta Inpharmatics LLC, Seattle, Washington, United States of America
PLoS ONE 6:e20660. 2011..Together, these results confirm that SNPs, reflecting recent genetic variation, occur more frequently in regions with less evolutionarily conservation...
Pathway and gene-set activation measurement from mRNA expression data: the tissue distribution of human pathwaysDavid M Levine
Rosetta Inpharmatics LLC, Merck and Co, Inc, Terry Avenue North, Seattle, WA 98109, USA
Genome Biol 7:R93. 2006..We also provide a comprehensive picture of the tissue distribution of human gene pathways and a useful public archive of human pathway expression data...
Digital transcriptome profiling using selective hexamer priming for cDNA synthesisChristopher D Armour
Department of Molecular Informatics, Rosetta Inpharmatics LLC, a wholly owned subsidiary of Merck and Co, Inc, Seattle, Washington, USA
Nat Methods 6:647-9. 2009..In this study we validated the technique by profiling human whole brain and universal human reference RNA using ultra-high-throughput sequencing...
Evolutionarily conserved and diverged alternative splicing events show different expression and functional profilesZhengyan Kan
Rosetta Inpharmatics LLC, Merck and Co, Inc. 401 Terry Ave N, Seattle, WA 98109, USA
Nucleic Acids Res 33:5659-66. 2005....
