Alexander Serganov

Summary

Affiliation: Memorial Sloan-Kettering Cancer Center
Country: USA

Publications

  1. pmc The long and the short of riboswitches
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Curr Opin Struct Biol 19:251-9. 2009
  2. pmc Amino acid recognition and gene regulation by riboswitches
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Biochim Biophys Acta 1789:592-611. 2009
  3. ncbi request reprint Ribozymes, riboswitches and beyond: regulation of gene expression without proteins
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nat Rev Genet 8:776-90. 2007
  4. pmc Towards deciphering the principles underlying an mRNA recognition code
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Curr Opin Struct Biol 18:120-9. 2008
  5. ncbi request reprint Determination of riboswitch structures: light at the end of the tunnel?
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA
    RNA Biol 7:98-103. 2010
  6. pmc Coenzyme recognition and gene regulation by a flavin mononucleotide riboswitch
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
    Nature 458:233-7. 2009
  7. pmc Structural insights into amino acid binding and gene control by a lysine riboswitch
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
    Nature 455:1263-7. 2008
  8. ncbi request reprint Structural basis for Diels-Alder ribozyme-catalyzed carbon-carbon bond formation
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nat Struct Mol Biol 12:218-24. 2005
  9. ncbi request reprint Structural basis for discriminative regulation of gene expression by adenine- and guanine-sensing mRNAs
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Chem Biol 11:1729-41. 2004
  10. doi request reprint Preparation and crystallization of riboswitch-ligand complexes
    Olga Pikovskaya
    Memorial Sloan Kettering Cancer Center, Department of Structural Biology, New York, NY 10021, USA
    Methods Mol Biol 540:115-28. 2009

Collaborators

Detail Information

Publications32

  1. pmc The long and the short of riboswitches
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Curr Opin Struct Biol 19:251-9. 2009
    ....
  2. pmc Amino acid recognition and gene regulation by riboswitches
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Biochim Biophys Acta 1789:592-611. 2009
    ..We also discuss the contribution of lysine riboswitches to antibiotic resistance and outline potential applications of riboswitches in biotechnology and medicine...
  3. ncbi request reprint Ribozymes, riboswitches and beyond: regulation of gene expression without proteins
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nat Rev Genet 8:776-90. 2007
    ..These findings have implications for understanding how cellular functions might have evolved from RNA-based origins...
  4. pmc Towards deciphering the principles underlying an mRNA recognition code
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Curr Opin Struct Biol 18:120-9. 2008
    ..In this review, we have highlighted recent structural studies that illustrate the diversity of recognition principles used by mRNA binders for timely and specific targeting and processing of the message...
  5. ncbi request reprint Determination of riboswitch structures: light at the end of the tunnel?
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA
    RNA Biol 7:98-103. 2010
    ....
  6. pmc Coenzyme recognition and gene regulation by a flavin mononucleotide riboswitch
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
    Nature 458:233-7. 2009
    ..Our studies also explain the effects of spontaneous and antibiotic-induced deregulatory mutations and provided molecular insights into FMN-based control of gene expression in normal and riboflavin-overproducing bacterial strains...
  7. pmc Structural insights into amino acid binding and gene control by a lysine riboswitch
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
    Nature 455:1263-7. 2008
    ....
  8. ncbi request reprint Structural basis for Diels-Alder ribozyme-catalyzed carbon-carbon bond formation
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nat Struct Mol Biol 12:218-24. 2005
    ..We observe structural parallels in the independently evolved catalytic pocket architectures for ribozyme- and antibody-catalyzed Diels-Alder carbon-carbon bond-forming reactions...
  9. ncbi request reprint Structural basis for discriminative regulation of gene expression by adenine- and guanine-sensing mRNAs
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Chem Biol 11:1729-41. 2004
    ..Recognition specificity is associated with Watson-Crick pairing of the encapsulated adenine and guanine ligands with uridine and cytosine, respectively...
  10. doi request reprint Preparation and crystallization of riboswitch-ligand complexes
    Olga Pikovskaya
    Memorial Sloan Kettering Cancer Center, Department of Structural Biology, New York, NY 10021, USA
    Methods Mol Biol 540:115-28. 2009
    ..The chapter describes the principles of construct design, in vitro transcription, RNA purification, complex formation, and crystallization screening utilized during the successful crystallization of several riboswitches...
  11. pmc Structure-function studies of FMRP RGG peptide recognition of an RNA duplex-quadruplex junction
    Anh Tuân Phan
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA
    Nat Struct Mol Biol 18:796-804. 2011
    ..These findings on FMRP RGG domain recognition by a combination of G-quadruplex and surrounding RNA sequences have implications for the recognition of other genomic G-rich RNAs...
  12. pmc Structural insights into ligand recognition by a sensing domain of the cooperative glycine riboswitch
    Lili Huang
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Mol Cell 40:774-86. 2010
    ....
  13. pmc Ribosomal protein S15 represses its own translation via adaptation of an rRNA-like fold within its mRNA
    Alexander Serganov
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    EMBO J 22:1898-908. 2003
    ..Our results highlight an astonishing plasticity of mRNA in its ability to adapt to evolutionary constraints, that contrasts with the extreme conservation of the rRNA-binding site...
  14. ncbi request reprint Argininamide binding arrests global motions in HIV-1 TAR RNA: comparison with Mg2+-induced conformational stabilization
    Stephen W Pitt
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    J Mol Biol 338:7-16. 2004
    ..The combined conformational analysis of TAR under three states reveals that ligands and divalent ions can stabilize similar RNA global conformations through distinct interactions involving different hydrogen bond alignments in the RNA...
  15. doi request reprint A decade of riboswitches
    Alexander Serganov
    Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY 10016, USA
    Cell 152:17-24. 2013
    ....
  16. pmc Structural principles of nucleoside selectivity in a 2'-deoxyguanosine riboswitch
    Olga Pikovskaya
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA
    Nat Chem Biol 7:748-55. 2011
    ....
  17. doi request reprint Metabolite recognition principles and molecular mechanisms underlying riboswitch function
    Alexander Serganov
    Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, New York 10016, USA
    Annu Rev Biophys 41:343-70. 2012
    ..These studies have greatly facilitated the dissection of molecular mechanisms underlying riboswitch action and should in turn guide the anticipated development of tools for manipulating gene regulatory circuits...
  18. doi request reprint Structural insights into ligand binding and gene expression control by an adenosylcobalamin riboswitch
    Alla Peselis
    Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, New York, USA
    Nat Struct Mol Biol 19:1182-4. 2012
    ....
  19. pmc Molecular recognition and function of riboswitches
    Alexander Serganov
    Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, 550 First Ave, MSB 393, New York, NY 10016, USA
    Curr Opin Struct Biol 22:279-86. 2012
    ..These studies have greatly improved our understanding of molecular events in riboswitch-mediated gene expression control and provided the molecular basis for intervention into riboswitch-controlled genetic circuits...
  20. pmc Long-range pseudoknot interactions dictate the regulatory response in the tetrahydrofolate riboswitch
    Lili Huang
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 108:14801-6. 2011
    ..Given that modern antibiotics target folate metabolism, the THF riboswitch structure provides insights on mechanistic aspects of riboswitch function and may help in manipulating THF levels in pathogenic bacteria...
  21. ncbi request reprint Ribosome-associated factor Y adopts a fold resembling a double-stranded RNA binding domain scaffold
    Keqiong Ye
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, USA
    Eur J Biochem 269:5182-91. 2002
    ..It appears probable that functional properties of pY are not identical to those of dsRBDs, as the postulated RNA-binding site in pY does not coincide with the RNA-binding surface of the dsRBDs...
  22. ncbi request reprint Structured mRNAs regulate translation initiation by binding to the platform of the ribosome
    Stefano Marzi
    IGBMC Institute of Genetics and of Molecular and Cellular Biology, Department of Structural Biology and Genomics, Illkirch, F 67404 France
    Cell 130:1019-31. 2007
    ..Different types of mRNA structures may be accommodated during translation preinitiation and regulate gene expression by transiently stalling the ribosome...
  23. ncbi request reprint Syntheses of RNAs with up to 100 nucleotides containing site-specific 2'-methylseleno labels for use in X-ray crystallography
    Claudia Hobartner
    Institute of Organic Chemistry, Center for Molecular Biosciences CMBI, Leopold Franzens University, 6020 Innsbruck, Austria
    J Am Chem Soc 127:12035-45. 2005
    ..We point out that the approach documented here has been the chemical basis for the very recent structure determination of the Diels-Alder ribozyme which represents the first novel RNA fold that has been solved via its Se-derivatives...
  24. ncbi request reprint Synthesis, oxidation behavior, crystallization and structure of 2'-methylseleno guanosine containing RNAs
    Holger Moroder
    Institute of Organic Chemistry, Center for Molecular Biosciences CMBI, Leopold Franzens University, 6020 Innsbruck, Austria
    J Am Chem Soc 128:9909-18. 2006
    ..Moreover, we determined the crystal structures of model RNA helices and showed that the Se modification can affect crystal packing interactions, thus potentially expanding the possibilities for obtaining the best crystal form...
  25. ncbi request reprint Structural basis for gene regulation by a thiamine pyrophosphate-sensing riboswitch
    Alexander Serganov
    Nature 441:1167-71. 2006
    ..In addition, this structure provides insight into how folded RNAs can form precision binding pockets that rival those formed by protein genetic factors...
  26. ncbi request reprint Control of stereoselectivity in an enzymatic reaction by backdoor access
    Richard Wombacher
    Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg, 69120 Heidelberg, Germany
    Angew Chem Int Ed Engl 45:2469-72. 2006
  27. ncbi request reprint Molecular mimicry in translational regulation: the case of ribosomal protein S15
    Chantal Ehresmann
    UPR 9002 du CNRS, Institut de Biologie Moleculaire et Cellulaire, Strasbourg Cedex, France
    RNA Biol 1:66-73. 2004
    ..They also highlight the high plasticity of RNA to adapt to evolutionary constraints...
  28. ncbi request reprint Specific recognition of rpsO mRNA and 16S rRNA by Escherichia coli ribosomal protein S15 relies on both mimicry and site differentiation
    Nathalie Mathy
    UPR9073 du CNRS, Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France
    Mol Microbiol 52:661-75. 2004
    ..However, specific S15-rpsO mRNA interactions can also be found, probably with A(-46) in loop L1 of the pseudoknot, demonstrating that mimicry between the two targets is limited...
  29. ncbi request reprint Probing motions between equivalent RNA domains using magnetic field induced residual dipolar couplings: accounting for correlations between motions and alignment
    Qi Zhang
    Department of Chemistry and Biophysics Research Division, University of Michigan, Ann Arbor, MI 48109, USA
    J Am Chem Soc 125:10530-1. 2003
    ....
  30. ncbi request reprint Crystallization of RNA/protein complexes
    Maria Garber
    Institute of Protein Research Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia
    Acta Crystallogr D Biol Crystallogr 58:1664-9. 2002
    ..In this paper we describe findings and methodical details, which helped us to succeed in obtaining X-ray quality crystals of several RNA/protein complexes...
  31. ncbi request reprint Do mRNA and rRNA binding sites of E.coli ribosomal protein S15 share common structural determinants?
    Alexander Serganov
    UPR 9002 du CNRS, Institut de Biologie Moleculaire et Cellulaire, 15 rue Rene Descartes, 67084 Cedex, Strasbourg, France
    J Mol Biol 320:963-78. 2002
    ..Otherwise, unique specific features are utilized, such as the three-way junction in the case of 16S rRNA and the looped out A(-46) for the mRNA pseudoknot...
  32. pmc Sequencing of flagellin genes from Natrialba magadii provides new insight into evolutionary aspects of archaeal flagellins
    Inna Serganova
    Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia
    J Bacteriol 184:318-22. 2002
    ..Based on the sequence analysis we suggest that different regions of the genes might have distinct evolutionary histories including possible genetic exchange with bacterial flagellin genes...