Research Topics
| Alexander SerganovSummaryAffiliation: Memorial Sloan-Kettering Cancer Center Country: USA Publications
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Detail Information
Publications
The long and the short of riboswitchesAlexander Serganov
Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
Curr Opin Struct Biol 19:251-9. 2009....
Amino acid recognition and gene regulation by riboswitchesAlexander Serganov
Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
Biochim Biophys Acta 1789:592-611. 2009..We also discuss the contribution of lysine riboswitches to antibiotic resistance and outline potential applications of riboswitches in biotechnology and medicine...
Ribozymes, riboswitches and beyond: regulation of gene expression without proteinsAlexander Serganov
Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
Nat Rev Genet 8:776-90. 2007..These findings have implications for understanding how cellular functions might have evolved from RNA-based origins...- Towards deciphering the principles underlying an mRNA recognition codeAlexander Serganov
Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
Curr Opin Struct Biol 18:120-9. 2008..In this review, we have highlighted recent structural studies that illustrate the diversity of recognition principles used by mRNA binders for timely and specific targeting and processing of the message... - Determination of riboswitch structures: light at the end of the tunnel?Alexander Serganov
Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA
RNA Biol 7:98-103. 2010.... - Coenzyme recognition and gene regulation by a flavin mononucleotide riboswitchAlexander Serganov
Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
Nature 458:233-7. 2009..Our studies also explain the effects of spontaneous and antibiotic-induced deregulatory mutations and provided molecular insights into FMN-based control of gene expression in normal and riboflavin-overproducing bacterial strains... - Structural insights into amino acid binding and gene control by a lysine riboswitchAlexander Serganov
Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
Nature 455:1263-7. 2008.... - Structural basis for Diels-Alder ribozyme-catalyzed carbon-carbon bond formationAlexander Serganov
Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA
Nat Struct Mol Biol 12:218-24. 2005..We observe structural parallels in the independently evolved catalytic pocket architectures for ribozyme- and antibody-catalyzed Diels-Alder carbon-carbon bond-forming reactions... - Structural basis for discriminative regulation of gene expression by adenine- and guanine-sensing mRNAsAlexander Serganov
Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA
Chem Biol 11:1729-41. 2004..Recognition specificity is associated with Watson-Crick pairing of the encapsulated adenine and guanine ligands with uridine and cytosine, respectively... - Preparation and crystallization of riboswitch-ligand complexesOlga Pikovskaya
Memorial Sloan Kettering Cancer Center, Department of Structural Biology, New York, NY 10021, USA
Methods Mol Biol 540:115-28. 2009..The chapter describes the principles of construct design, in vitro transcription, RNA purification, complex formation, and crystallization screening utilized during the successful crystallization of several riboswitches... - Structure-function studies of FMRP RGG peptide recognition of an RNA duplex-quadruplex junctionAnh Tuân Phan
Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA
Nat Struct Mol Biol 18:796-804. 2011..These findings on FMRP RGG domain recognition by a combination of G-quadruplex and surrounding RNA sequences have implications for the recognition of other genomic G-rich RNAs... - Structural insights into ligand recognition by a sensing domain of the cooperative glycine riboswitchLili Huang
Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
Mol Cell 40:774-86. 2010.... - Ribosomal protein S15 represses its own translation via adaptation of an rRNA-like fold within its mRNAAlexander Serganov
Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
EMBO J 22:1898-908. 2003..Our results highlight an astonishing plasticity of mRNA in its ability to adapt to evolutionary constraints, that contrasts with the extreme conservation of the rRNA-binding site... - Argininamide binding arrests global motions in HIV-1 TAR RNA: comparison with Mg2+-induced conformational stabilizationStephen W Pitt
Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA
J Mol Biol 338:7-16. 2004..The combined conformational analysis of TAR under three states reveals that ligands and divalent ions can stabilize similar RNA global conformations through distinct interactions involving different hydrogen bond alignments in the RNA... - A decade of riboswitchesAlexander Serganov
Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY 10016, USA
Cell 152:17-24. 2013.... - Structural principles of nucleoside selectivity in a 2'-deoxyguanosine riboswitchOlga Pikovskaya
Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA
Nat Chem Biol 7:748-55. 2011.... - Metabolite recognition principles and molecular mechanisms underlying riboswitch functionAlexander Serganov
Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, New York 10016, USA
Annu Rev Biophys 41:343-70. 2012..These studies have greatly facilitated the dissection of molecular mechanisms underlying riboswitch action and should in turn guide the anticipated development of tools for manipulating gene regulatory circuits... - Structural insights into ligand binding and gene expression control by an adenosylcobalamin riboswitchAlla Peselis
Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, New York, USA
Nat Struct Mol Biol 19:1182-4. 2012.... - Molecular recognition and function of riboswitchesAlexander Serganov
Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, 550 First Ave, MSB 393, New York, NY 10016, USA
Curr Opin Struct Biol 22:279-86. 2012..These studies have greatly improved our understanding of molecular events in riboswitch-mediated gene expression control and provided the molecular basis for intervention into riboswitch-controlled genetic circuits... - Long-range pseudoknot interactions dictate the regulatory response in the tetrahydrofolate riboswitchLili Huang
Structural Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA
Proc Natl Acad Sci U S A 108:14801-6. 2011..Given that modern antibiotics target folate metabolism, the THF riboswitch structure provides insights on mechanistic aspects of riboswitch function and may help in manipulating THF levels in pathogenic bacteria... - Ribosome-associated factor Y adopts a fold resembling a double-stranded RNA binding domain scaffoldKeqiong Ye
Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, USA
Eur J Biochem 269:5182-91. 2002..It appears probable that functional properties of pY are not identical to those of dsRBDs, as the postulated RNA-binding site in pY does not coincide with the RNA-binding surface of the dsRBDs... - Structured mRNAs regulate translation initiation by binding to the platform of the ribosomeStefano Marzi
IGBMC Institute of Genetics and of Molecular and Cellular Biology, Department of Structural Biology and Genomics, Illkirch, F 67404 France
Cell 130:1019-31. 2007..Different types of mRNA structures may be accommodated during translation preinitiation and regulate gene expression by transiently stalling the ribosome... - Syntheses of RNAs with up to 100 nucleotides containing site-specific 2'-methylseleno labels for use in X-ray crystallographyClaudia Hobartner
Institute of Organic Chemistry, Center for Molecular Biosciences CMBI, Leopold Franzens University, 6020 Innsbruck, Austria
J Am Chem Soc 127:12035-45. 2005..We point out that the approach documented here has been the chemical basis for the very recent structure determination of the Diels-Alder ribozyme which represents the first novel RNA fold that has been solved via its Se-derivatives... - Synthesis, oxidation behavior, crystallization and structure of 2'-methylseleno guanosine containing RNAsHolger Moroder
Institute of Organic Chemistry, Center for Molecular Biosciences (CMBI, Leopold-Franzens University, 6020 Innsbruck, Austria
J Am Chem Soc 128:9909-18. 2006..Moreover, we determined the crystal structures of model RNA helices and showed that the Se modification can affect crystal packing interactions, thus potentially expanding the possibilities for obtaining the best crystal form... - Structural basis for gene regulation by a thiamine pyrophosphate-sensing riboswitchAlexander Serganov
Nature 441:1167-71. 2006..In addition, this structure provides insight into how folded RNAs can form precision binding pockets that rival those formed by protein genetic factors... - Control of stereoselectivity in an enzymatic reaction by backdoor accessRichard Wombacher
Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg, 69120 Heidelberg, Germany
Angew Chem Int Ed Engl 45:2469-72. 2006 - Molecular mimicry in translational regulation: the case of ribosomal protein S15Chantal Ehresmann
UPR 9002 du CNRS, Institut de Biologie Moleculaire et Cellulaire, Strasbourg Cedex, France
RNA Biol 1:66-73. 2004..They also highlight the high plasticity of RNA to adapt to evolutionary constraints... - Specific recognition of rpsO mRNA and 16S rRNA by Escherichia coli ribosomal protein S15 relies on both mimicry and site differentiationNathalie Mathy
UPR9073 du CNRS, Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France
Mol Microbiol 52:661-75. 2004..However, specific S15-rpsO mRNA interactions can also be found, probably with A(-46) in loop L1 of the pseudoknot, demonstrating that mimicry between the two targets is limited... - Probing motions between equivalent RNA domains using magnetic field induced residual dipolar couplings: accounting for correlations between motions and alignmentQi Zhang
Department of Chemistry and Biophysics Research Division, University of Michigan, Ann Arbor, MI 48109, USA
J Am Chem Soc 125:10530-1. 2003.... - Crystallization of RNA/protein complexesMaria Garber
Institute of Protein Research Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia
Acta Crystallogr D Biol Crystallogr 58:1664-9. 2002..In this paper we describe findings and methodical details, which helped us to succeed in obtaining X-ray quality crystals of several RNA/protein complexes... - Do mRNA and rRNA binding sites of E.coli ribosomal protein S15 share common structural determinants?Alexander Serganov
UPR 9002 du CNRS, Institut de Biologie Moleculaire et Cellulaire, 15 rue Rene Descartes, 67084 Cedex, Strasbourg, France
J Mol Biol 320:963-78. 2002..Otherwise, unique specific features are utilized, such as the three-way junction in the case of 16S rRNA and the looped out A(-46) for the mRNA pseudoknot... - Sequencing of flagellin genes from Natrialba magadii provides new insight into evolutionary aspects of archaeal flagellinsInna Serganova
Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia
J Bacteriol 184:318-22. 2002..Based on the sequence analysis we suggest that different regions of the genes might have distinct evolutionary histories including possible genetic exchange with bacterial flagellin genes...