KENNETH MARIANS

Summary

Affiliation: Memorial Sloan-Kettering Cancer Center
Country: USA

Publications

  1. ncbi request reprint Replisome assembly and the direct restart of stalled replication forks
    Ryan C Heller
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nat Rev Mol Cell Biol 7:932-43. 2006
  2. ncbi request reprint Helicase structures: a new twist on DNA unwinding
    K J Marians
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Structure 5:1129-34. 1997
  3. pmc Mechanisms of replication fork restart in Escherichia coli
    Kenneth J Marians
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Philos Trans R Soc Lond B Biol Sci 359:71-7. 2004
  4. ncbi request reprint Crawling and wiggling on DNA: structural insights to the mechanism of DNA unwinding by helicases
    K J Marians
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Structure 8:R227-35. 2000
  5. ncbi request reprint PriA-directed replication fork restart in Escherichia coli
    K J Marians
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Trends Biochem Sci 25:185-9. 2000
  6. ncbi request reprint Replication and recombination intersect
    K J Marians
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Curr Opin Genet Dev 10:151-6. 2000
  7. ncbi request reprint Replication fork reactivation downstream of a blocked nascent leading strand
    Ryan C Heller
    Molecular Biology Program, Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
    Nature 439:557-62. 2006
  8. ncbi request reprint PriA mediates DNA replication pathway choice at recombination intermediates
    Liewei Xu
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Mol Cell 11:817-26. 2003
  9. ncbi request reprint The disposition of nascent strands at stalled replication forks dictates the pathway of replisome loading during restart
    Ryan C Heller
    Program in Molecular Biology, Weill Graduate School of Medical Sciences, Cornell University, New York, New York 10021, USA
    Mol Cell 17:733-43. 2005
  10. ncbi request reprint Non-replicative helicases at the replication fork
    Ryan C Heller
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    DNA Repair (Amst) 6:945-52. 2007

Research Grants

  1. Topoisomerases and Chromosome Segregation
    Kenneth J Marians; Fiscal Year: 2010
  2. Topoisomerases and Chromosome Segregation
    Kenneth J Marians; Fiscal Year: 2010
  3. CONFERENCE ON DNA REPLICATION AND RECOMBINATION
    KENNETH MARIANS; Fiscal Year: 2002
  4. TOPOISOMERASES AND DNA REPLICATION
    KENNETH MARIANS; Fiscal Year: 2002
  5. Topoisomerases and Chromosome Segregation
    KENNETH MARIANS; Fiscal Year: 2007
  6. Integrated PhD Training Program in Cancer Biology
    KENNETH MARIANS; Fiscal Year: 2007
  7. MECHANISMS OF DNA REPLICATION
    KENNETH MARIANS; Fiscal Year: 2007
  8. Topoisomerases and Chromosome Segregation
    KENNETH MARIANS; Fiscal Year: 2009
  9. ROLE OF TOPOISOMERASES IN DNA METABOLISM
    KENNETH MARIANS; Fiscal Year: 1993
  10. ROLE OF TOPOISOMERASES IN DNA REPLICATION
    KENNETH MARIANS; Fiscal Year: 1990

Collaborators

  • Olivier Espeli
  • Carolina B Gabbai
  • Ryan C Heller
  • Ryo Hayama
  • Ram Madabhushi
  • Catherine Suski
  • Pearl Nurse
  • Liewei Xu
  • Abu Amar M Al Mamun
  • Cindy Levine
  • Heide Hassing
  • M Zafri Humayun

Detail Information

Publications22

  1. ncbi request reprint Replisome assembly and the direct restart of stalled replication forks
    Ryan C Heller
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nat Rev Mol Cell Biol 7:932-43. 2006
    ..Some recent biochemical observations support models of direct fork repair in which the removal of the blocking template lesion is not always required for replication restart...
  2. ncbi request reprint Helicase structures: a new twist on DNA unwinding
    K J Marians
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Structure 5:1129-34. 1997
    ..These structures illuminate the roles of the conserved helicase motifs in catalytic function and offer clues as to how these proteins can translocate along DNA...
  3. pmc Mechanisms of replication fork restart in Escherichia coli
    Kenneth J Marians
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Philos Trans R Soc Lond B Biol Sci 359:71-7. 2004
    ..These reactions have been reconstituted with purified recombination and replication proteins...
  4. ncbi request reprint Crawling and wiggling on DNA: structural insights to the mechanism of DNA unwinding by helicases
    K J Marians
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Structure 8:R227-35. 2000
    ..These structures have led to the elaboration of the first molecular models to describe DNA helicase action...
  5. ncbi request reprint PriA-directed replication fork restart in Escherichia coli
    K J Marians
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Trends Biochem Sci 25:185-9. 2000
    ..Recent evidence shows that replication fork restart is effected by the action of the recombination proteins generating a substrate for PriA-directed replication fork assembly...
  6. ncbi request reprint Replication and recombination intersect
    K J Marians
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Curr Opin Genet Dev 10:151-6. 2000
    ..Double-strand break repair in yeast has been shown to require both leading- and lagging-strand DNA synthesis. These observations suggest that the recombination and replication machinery cooperate to maintain genomic integrity...
  7. ncbi request reprint Replication fork reactivation downstream of a blocked nascent leading strand
    Ryan C Heller
    Molecular Biology Program, Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
    Nature 439:557-62. 2006
    ....
  8. ncbi request reprint PriA mediates DNA replication pathway choice at recombination intermediates
    Liewei Xu
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Mol Cell 11:817-26. 2003
    ....
  9. ncbi request reprint The disposition of nascent strands at stalled replication forks dictates the pathway of replisome loading during restart
    Ryan C Heller
    Program in Molecular Biology, Weill Graduate School of Medical Sciences, Cornell University, New York, New York 10021, USA
    Mol Cell 17:733-43. 2005
    ..These observations suggest that the type of initial damage on the DNA template and how the inactivated fork is processed ultimately influence the choice of enzymatic restart pathway...
  10. ncbi request reprint Non-replicative helicases at the replication fork
    Ryan C Heller
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    DNA Repair (Amst) 6:945-52. 2007
    ....
  11. ncbi request reprint Unwinding of the nascent lagging strand by Rep and PriA enables the direct restart of stalled replication forks
    Ryan C Heller
    Programs in Molecular Biology, Weill Graduate School of Medical Sciences of Cornell University and Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 280:34143-51. 2005
    ..Direct rescue of replication forks by the Rep-PriC and PriA-PriC pathways in this manner may contribute to genomic stability by avoiding the potential dangers of fork breakage inherent to recombination-dependent restart pathways...
  12. pmc Physical and functional interaction between the condensin MukB and the decatenase topoisomerase IV in Escherichia coli
    Ryo Hayama
    Weill Cornell Graduate School of Medical Sciences, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 107:18826-31. 2010
    ..We show that MukB stimulates the superhelical DNA relaxation activity of wild-type Topo IV, but not that of Topo IV reconstituted with ParC R705E R729A...
  13. ncbi request reprint Temporal regulation of topoisomerase IV activity in E. coli
    Olivier Espeli
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Mol Cell 11:189-201. 2003
    ..These observations suggest that topoisomerase IV activity in vivo might be dependent on release of ParC from the replication factory...
  14. pmc Recruitment to stalled replication forks of the PriA DNA helicase and replisome-loading activities is essential for survival
    Carolina B Gabbai
    Molecular Biology Program, Weill Cornell Graduate School of Medical Sciences, New York, NY, USA
    DNA Repair (Amst) 9:202-9. 2010
    ..This review focuses on the activities of PriA and its role in replication fork assembly and maintaining genomic integrity...
  15. pmc Resolution of converging replication forks by RecQ and topoisomerase III
    Catherine Suski
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Mol Cell 30:779-89. 2008
    ..This resolution reaction is specific for the RecQ-topoisomerase III pair and is mediated by interaction of both of these enzymes with the single-stranded DNA-binding protein SSB...
  16. ncbi request reprint SetB: an integral membrane protein that affects chromosome segregation in Escherichia coli
    Olivier Espeli
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Mol Microbiol 50:495-509. 2003
    ..SetB localizes in the cell as a helix and interacts with MreB, the bacterial actin homologue, which also forms a helix. These observations suggest that there may be a link between chromosome segregation and cellular infrastructure...
  17. ncbi request reprint A physical and functional interaction between Escherichia coli FtsK and topoisomerase IV
    Olivier Espeli
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 278:44639-44. 2003
    ..The interaction between FtsK and Topo IV may provide a means for concentrating the latter enzyme at the cell center...
  18. ncbi request reprint Topoisomerase III can serve as the cellular decatenase in Escherichia coli
    Pearl Nurse
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 278:8653-60. 2003
    ..These observations suggest that precatenane unlinking is sufficient to sustain decatenation of replicating daughter chromosomes in the cell...
  19. pmc Actin homolog MreB affects chromosome segregation by regulating topoisomerase IV in Escherichia coli
    Ram Madabhushi
    Program in Molecular Biology, Weill Graduate School of Cornell University, New York, NY 10065, USA
    Mol Cell 33:171-80. 2009
    ..In addition, MreB physically interacts with the ParC subunit of Topo IV. Together, these results may explain how dynamics of the bacterial cytoskeleton are coordinated with the timing of chromosome segregation...
  20. doi request reprint Understanding how the replisome works
    Kenneth J Marians
    Nat Struct Mol Biol 15:125-7. 2008
  21. ncbi request reprint DNA polymerase III from Escherichia coli cells expressing mutA mistranslator tRNA is error-prone
    Abu Amar M Al Mamun
    University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Department of Microbiology and Molecular Genetics, International Center for Public Health, Newark, New Jersey 07101 1709, USA
    J Biol Chem 277:46319-27. 2002
    ..These findings suggest that DNA polymerase III is modified in TSM-induced cells...
  22. ncbi request reprint Untangling intracellular DNA topology
    Olivier Espeli
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Mol Microbiol 52:925-31. 2004
    ....

Research Grants30

  1. Topoisomerases and Chromosome Segregation
    Kenneth J Marians; Fiscal Year: 2010
    ..We anticipate that as we understand more about these pathways and more of the participants are revealed, potential new targets for antimicrobial chemotherapy will be presented. ..
  2. Topoisomerases and Chromosome Segregation
    Kenneth J Marians; Fiscal Year: 2010
    ..We anticipate that as we understand more about these pathways and more of the participants are revealed, potential new targets for antimicrobial chemotherapy will be presented. ..
  3. CONFERENCE ON DNA REPLICATION AND RECOMBINATION
    KENNETH MARIANS; Fiscal Year: 2002
    ..Given the rapid pace of progress in these fields over the last three years, it should prove to be a timely and exciting meeting. ..
  4. TOPOISOMERASES AND DNA REPLICATION
    KENNETH MARIANS; Fiscal Year: 2002
    ..Biochemical techniques will be used to isolate the proteins responsible for double-strand break-formation at these nonreversible complexes. ..
  5. Topoisomerases and Chromosome Segregation
    KENNETH MARIANS; Fiscal Year: 2007
    ..the molecular mechanisms underlying temporal regulation of Topo IV activity in the cell? What is the role of the Topo IV-FtsK interaction in chromosome segregation? And, what is the role of SpcA in chromosome segregation? ..
  6. Integrated PhD Training Program in Cancer Biology
    KENNETH MARIANS; Fiscal Year: 2007
    ..The faculty of the Gerstner Sloan-Kettering Graduate School are drawn from both the basic science and clinical arms of the Cancer Center. ..
  7. MECHANISMS OF DNA REPLICATION
    KENNETH MARIANS; Fiscal Year: 2007
    ..abstract_text> ..
  8. Topoisomerases and Chromosome Segregation
    KENNETH MARIANS; Fiscal Year: 2009
    ..We anticipate that as we understand more about these pathways and more of the participants are revealed, potential new targets for antimicrobial chemotherapy will be presented. ..
  9. ROLE OF TOPOISOMERASES IN DNA METABOLISM
    KENNETH MARIANS; Fiscal Year: 1993
    ....
  10. ROLE OF TOPOISOMERASES IN DNA REPLICATION
    KENNETH MARIANS; Fiscal Year: 1990
    ....