Research Topics
Genomes and Genes | KENNETH MARIANSSummaryAffiliation: Memorial Sloan-Kettering Cancer Center Country: USA Publications
Research Grants
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Detail Information
Publications
Replisome assembly and the direct restart of stalled replication forksRyan C Heller
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA
Nat Rev Mol Cell Biol 7:932-43. 2006..Some recent biochemical observations support models of direct fork repair in which the removal of the blocking template lesion is not always required for replication restart...- Helicase structures: a new twist on DNA unwindingK J Marians
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
Structure 5:1129-34. 1997..These structures illuminate the roles of the conserved helicase motifs in catalytic function and offer clues as to how these proteins can translocate along DNA... 
Mechanisms of replication fork restart in Escherichia coliKenneth J Marians
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
Philos Trans R Soc Lond B Biol Sci 359:71-7. 2004..These reactions have been reconstituted with purified recombination and replication proteins...- Crawling and wiggling on DNA: structural insights to the mechanism of DNA unwinding by helicasesK J Marians
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
Structure 8:R227-35. 2000..These structures have led to the elaboration of the first molecular models to describe DNA helicase action... - PriA-directed replication fork restart in Escherichia coliK J Marians
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
Trends Biochem Sci 25:185-9. 2000..Recent evidence shows that replication fork restart is effected by the action of the recombination proteins generating a substrate for PriA-directed replication fork assembly... - Replication and recombination intersectK J Marians
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
Curr Opin Genet Dev 10:151-6. 2000..Double-strand break repair in yeast has been shown to require both leading- and lagging-strand DNA synthesis. These observations suggest that the recombination and replication machinery cooperate to maintain genomic integrity... - Replication fork reactivation downstream of a blocked nascent leading strandRyan C Heller
Molecular Biology Program, Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
Nature 439:557-62. 2006.... - PriA mediates DNA replication pathway choice at recombination intermediatesLiewei Xu
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
Mol Cell 11:817-26. 2003.... - The disposition of nascent strands at stalled replication forks dictates the pathway of replisome loading during restartRyan C Heller
Program in Molecular Biology, Weill Graduate School of Medical Sciences, Cornell University, New York, New York 10021, USA
Mol Cell 17:733-43. 2005..These observations suggest that the type of initial damage on the DNA template and how the inactivated fork is processed ultimately influence the choice of enzymatic restart pathway... - Non-replicative helicases at the replication forkRyan C Heller
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
DNA Repair (Amst) 6:945-52. 2007.... - Unwinding of the nascent lagging strand by Rep and PriA enables the direct restart of stalled replication forksRyan C Heller
Programs in Molecular Biology, Weill Graduate School of Medical Sciences of Cornell University and Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
J Biol Chem 280:34143-51. 2005..Direct rescue of replication forks by the Rep-PriC and PriA-PriC pathways in this manner may contribute to genomic stability by avoiding the potential dangers of fork breakage inherent to recombination-dependent restart pathways... - Physical and functional interaction between the condensin MukB and the decatenase topoisomerase IV in Escherichia coliRyo Hayama
Weill Cornell Graduate School of Medical Sciences, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
Proc Natl Acad Sci U S A 107:18826-31. 2010..We show that MukB stimulates the superhelical DNA relaxation activity of wild-type Topo IV, but not that of Topo IV reconstituted with ParC R705E R729A... - Temporal regulation of topoisomerase IV activity in E. coliOlivier Espeli
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
Mol Cell 11:189-201. 2003..These observations suggest that topoisomerase IV activity in vivo might be dependent on release of ParC from the replication factory... - Recruitment to stalled replication forks of the PriA DNA helicase and replisome-loading activities is essential for survivalCarolina B Gabbai
Molecular Biology Program, Weill Cornell Graduate School of Medical Sciences, New York, NY, USA
DNA Repair (Amst) 9:202-9. 2010..This review focuses on the activities of PriA and its role in replication fork assembly and maintaining genomic integrity... - Resolution of converging replication forks by RecQ and topoisomerase IIICatherine Suski
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
Mol Cell 30:779-89. 2008..This resolution reaction is specific for the RecQ-topoisomerase III pair and is mediated by interaction of both of these enzymes with the single-stranded DNA-binding protein SSB... - SetB: an integral membrane protein that affects chromosome segregation in Escherichia coliOlivier Espeli
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
Mol Microbiol 50:495-509. 2003..SetB localizes in the cell as a helix and interacts with MreB, the bacterial actin homologue, which also forms a helix. These observations suggest that there may be a link between chromosome segregation and cellular infrastructure... - A physical and functional interaction between Escherichia coli FtsK and topoisomerase IVOlivier Espeli
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
J Biol Chem 278:44639-44. 2003..The interaction between FtsK and Topo IV may provide a means for concentrating the latter enzyme at the cell center... - Topoisomerase III can serve as the cellular decatenase in Escherichia coliPearl Nurse
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
J Biol Chem 278:8653-60. 2003..These observations suggest that precatenane unlinking is sufficient to sustain decatenation of replicating daughter chromosomes in the cell... - Actin homolog MreB affects chromosome segregation by regulating topoisomerase IV in Escherichia coliRam Madabhushi
Program in Molecular Biology, Weill Graduate School of Cornell University, New York, NY 10065, USA
Mol Cell 33:171-80. 2009..In addition, MreB physically interacts with the ParC subunit of Topo IV. Together, these results may explain how dynamics of the bacterial cytoskeleton are coordinated with the timing of chromosome segregation... - Understanding how the replisome worksKenneth J Marians
Nat Struct Mol Biol 15:125-7. 2008 - DNA polymerase III from Escherichia coli cells expressing mutA mistranslator tRNA is error-proneAbu Amar M Al Mamun
University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Department of Microbiology and Molecular Genetics, International Center for Public Health, Newark, New Jersey 07101-1709, USA
J Biol Chem 277:46319-27. 2002..These findings suggest that DNA polymerase III is modified in TSM-induced cells... - Untangling intracellular DNA topologyOlivier Espeli
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA
Mol Microbiol 52:925-31. 2004....
Research Grants
- Topoisomerases and Chromosome SegregationKenneth J Marians; Fiscal Year: 2010..We anticipate that as we understand more about these pathways and more of the participants are revealed, potential new targets for antimicrobial chemotherapy will be presented. ..
- Topoisomerases and Chromosome SegregationKenneth J Marians; Fiscal Year: 2010..We anticipate that as we understand more about these pathways and more of the participants are revealed, potential new targets for antimicrobial chemotherapy will be presented. ..
- CONFERENCE ON DNA REPLICATION AND RECOMBINATIONKENNETH MARIANS; Fiscal Year: 2002..Given the rapid pace of progress in these fields over the last three years, it should prove to be a timely and exciting meeting. ..
- TOPOISOMERASES AND DNA REPLICATIONKENNETH MARIANS; Fiscal Year: 2002..Biochemical techniques will be used to isolate the proteins responsible for double-strand break-formation at these nonreversible complexes. ..
- Topoisomerases and Chromosome SegregationKENNETH MARIANS; Fiscal Year: 2007..the molecular mechanisms underlying temporal regulation of Topo IV activity in the cell? What is the role of the Topo IV-FtsK interaction in chromosome segregation? And, what is the role of SpcA in chromosome segregation? ..
- Integrated PhD Training Program in Cancer BiologyKENNETH MARIANS; Fiscal Year: 2007..The faculty of the Gerstner Sloan-Kettering Graduate School are drawn from both the basic science and clinical arms of the Cancer Center. ..
- MECHANISMS OF DNA REPLICATIONKENNETH MARIANS; Fiscal Year: 2007..abstract_text> ..
- Topoisomerases and Chromosome SegregationKENNETH MARIANS; Fiscal Year: 2009..We anticipate that as we understand more about these pathways and more of the participants are revealed, potential new targets for antimicrobial chemotherapy will be presented. ..
- ROLE OF TOPOISOMERASES IN DNA METABOLISMKENNETH MARIANS; Fiscal Year: 1993....
- ROLE OF TOPOISOMERASES IN DNA REPLICATIONKENNETH MARIANS; Fiscal Year: 1990....