S Keeney

Summary

Affiliation: Memorial Sloan-Kettering Cancer Center
Country: USA

Publications

  1. pmc ATM promotes the obligate XY crossover and both crossover control and chromosome axis integrity on autosomes
    Marco Barchi
    Developmental Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, United States of America
    PLoS Genet 4:e1000076. 2008
  2. pmc Histone methylation sets the stage for meiotic DNA breaks
    Ryan Kniewel
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    EMBO J 28:81-3. 2009
  3. pmc ATM controls meiotic double-strand-break formation
    Julian Lange
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York 10065, USA
    Nature 479:237-40. 2011
  4. pmc Mouse BAZ1A (ACF1) is dispensable for double-strand break repair but is essential for averting improper gene expression during spermatogenesis
    James A Dowdle
    Louis V Gerstner Jr Graduate School of Biomedical Sciences, New York, New York, United States of America Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, United States of America Howard Hughes Medical Institute, Memorial Sloan Kettering Cancer Center, New York, New York, United States of America
    PLoS Genet 9:e1003945. 2013
  5. pmc Meiotic recombination initiation in and around retrotransposable elements in Saccharomyces cerevisiae
    Mariko Sasaki
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, United States of America
    PLoS Genet 9:e1003732. 2013
  6. pmc Homeostatic control of recombination is implemented progressively in mouse meiosis
    Francesca Cole
    Developmental Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York 10065, USA
    Nat Cell Biol 14:424-30. 2012
  7. ncbi request reprint Initiation of meiotic recombination by formation of DNA double-strand breaks: mechanism and regulation
    S Keeney
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Ave, Box 97, New York, NY 10021, USA
    Biochem Soc Trans 34:523-5. 2006
  8. ncbi request reprint A mouse homolog of the Saccharomyces cerevisiae meiotic recombination DNA transesterase Spo11p
    S Keeney
    Molecular Biology Program, Cell Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York, 10021, USA
    Genomics 61:170-82. 1999
  9. ncbi request reprint Synaptonemal complex formation: where does it start?
    Kiersten A Henderson
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Bioessays 27:995-8. 2005
  10. ncbi request reprint Chromosome synapsis defects and sexually dimorphic meiotic progression in mice lacking Spo11
    F Baudat
    Cell Biology Program Memorial Sloan Kettering Cancer Center New York, NY 10021, USA
    Mol Cell 6:989-98. 2000

Collaborators

Detail Information

Publications41

  1. pmc ATM promotes the obligate XY crossover and both crossover control and chromosome axis integrity on autosomes
    Marco Barchi
    Developmental Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, United States of America
    PLoS Genet 4:e1000076. 2008
    ..Together, these findings indicate that ATM plays a role in both crossover control and chromosome axis integrity and further suggests that ATM is important for coordinating these features of meiotic chromosome dynamics...
  2. pmc Histone methylation sets the stage for meiotic DNA breaks
    Ryan Kniewel
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    EMBO J 28:81-3. 2009
    ..These findings have important implications for elucidating the previously recognized but little understood connections between meiotic break formation and transcriptional promoters in this organism...
  3. pmc ATM controls meiotic double-strand-break formation
    Julian Lange
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York 10065, USA
    Nature 479:237-40. 2011
    ..Our findings explain previously puzzling phenotypes of Atm-null mice and provide a molecular basis for the gonadal dysgenesis observed in ataxia telangiectasia, the human syndrome caused by ATM deficiency...
  4. pmc Mouse BAZ1A (ACF1) is dispensable for double-strand break repair but is essential for averting improper gene expression during spermatogenesis
    James A Dowdle
    Louis V Gerstner Jr Graduate School of Biomedical Sciences, New York, New York, United States of America Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, United States of America Howard Hughes Medical Institute, Memorial Sloan Kettering Cancer Center, New York, New York, United States of America
    PLoS Genet 9:e1003945. 2013
    ....
  5. pmc Meiotic recombination initiation in and around retrotransposable elements in Saccharomyces cerevisiae
    Mariko Sasaki
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, United States of America
    PLoS Genet 9:e1003732. 2013
    ....
  6. pmc Homeostatic control of recombination is implemented progressively in mouse meiosis
    Francesca Cole
    Developmental Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York 10065, USA
    Nat Cell Biol 14:424-30. 2012
    ..Meiotic recombination exemplifies how order can be progressively implemented in a self-organizing system despite natural cell-to-cell disparities in the underlying biochemical processes...
  7. ncbi request reprint Initiation of meiotic recombination by formation of DNA double-strand breaks: mechanism and regulation
    S Keeney
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Ave, Box 97, New York, NY 10021, USA
    Biochem Soc Trans 34:523-5. 2006
    ..We review here recent findings pertaining to protein-protein interactions important for DSB formation, the mechanism of an early step in the processing of Spo11-generated DSBs, and regulation of DSB formation by protein kinases...
  8. ncbi request reprint A mouse homolog of the Saccharomyces cerevisiae meiotic recombination DNA transesterase Spo11p
    S Keeney
    Molecular Biology Program, Cell Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York, 10021, USA
    Genomics 61:170-82. 1999
    ..Taken together, these results strongly suggest that this gene encodes the functional homolog of yeast Spo11p, which in turn suggests that the mechanism of meiotic recombination initiation is conserved between yeast and mammals...
  9. ncbi request reprint Synaptonemal complex formation: where does it start?
    Kiersten A Henderson
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Bioessays 27:995-8. 2005
    ..A recent study in budding yeast reveals an unexpected role in centromere pairing for a protein component of the synaptonemal complex, Zip1. These findings have implications for synaptonemal complex formation...
  10. ncbi request reprint Chromosome synapsis defects and sexually dimorphic meiotic progression in mice lacking Spo11
    F Baudat
    Cell Biology Program Memorial Sloan Kettering Cancer Center New York, NY 10021, USA
    Mol Cell 6:989-98. 2000
    ..Our results also support the view that mammalian checkpoint responses to meiotic recombination and/or synapsis defects are sexually dimorphic...
  11. ncbi request reprint Sex and the single (double-strand) break
    Emmanuelle Martini
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Mol Cell 9:700-2. 2002
    ..Two recent studies show that the fate of a single DSB in yeast is strongly influenced by the presence of other breaks in the genome, hinting that cell-wide or chromosome-regional mechanisms control the outcome of DSB repair...
  12. pmc Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae
    Shohreh Maleki
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Ave, New York, NY 10021, USA
    Chromosoma 116:471-86. 2007
    ..These studies also suggest that an essential function of Rec102 and Rec104 is to connect Mei4 and Rec114 to Spo11...
  13. ncbi request reprint Mechanism and control of meiotic recombination initiation
    S Keeney
    Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, and Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
    Curr Top Dev Biol 52:1-53. 2001
    ....
  14. pmc Functional interactions between SPO11 and REC102 during initiation of meiotic recombination in Saccharomyces cerevisiae
    Kehkooi Kee
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center and Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
    Genetics 160:111-22. 2002
    ..Tagged Rec102p localized to the nucleus in whole cells and to chromatin on spread meiotic chromosomes. Our results are consistent with the idea that a multiprotein complex that includes Spo11p and Rec102p promotes meiotic DSB formation...
  15. pmc Endonucleolytic processing of covalent protein-linked DNA double-strand breaks
    Matthew J Neale
    Molecular Biology Programs, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 436:1053-7. 2005
    ..Our findings suggest a general mechanism for repair of protein-linked DSBs...
  16. pmc Regulating the formation of DNA double-strand breaks in meiosis
    Hajime Murakami
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
    Genes Dev 22:286-92. 2008
  17. ncbi request reprint Meiotic recombination: Making and breaking go hand in hand
    F Baudat
    Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, Box 97, New York, New York 10021, USA
    Curr Biol 11:R45-8. 2001
    ..Recent studies suggest that the initiation of meiotic recombination is mechanistically coupled to premeiotic DNA replication...
  18. pmc Comprehensive, fine-scale dissection of homologous recombination outcomes at a hot spot in mouse meiosis
    Francesca Cole
    Developmental Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA
    Mol Cell 39:700-10. 2010
    ..Transmission distortion was observed in one hybrid, with NCOs providing a significant contribution. Thus, NCO recombination events play a substantial role in mammalian meiosis and genome evolution...
  19. pmc Evolutionary conservation of meiotic DSB proteins: more than just Spo11
    Francesca Cole
    Developmental Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
    Genes Dev 24:1201-7. 2010
    ..1266-1280) used a phylogenomic approach to identify two of these proteins across multiple clades, and confirmed that one of these, MEI4, is a functional ortholog in mouse...
  20. pmc Spatial organization and dynamics of the association of Rec102 and Rec104 with meiotic chromosomes
    Kehkooi Kee
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center and Weill Graduate School of Medical Sciences of Cornell University, New York, NY 10021, USA
    EMBO J 23:1815-24. 2004
    ..These studies reveal unexpected behaviors for Rec102 and Rec104, and point to distinct roles and subcomplexes among the DSB proteins...
  21. pmc Identification of residues in yeast Spo11p critical for meiotic DNA double-strand break formation
    Robert L Diaz
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Mol Cell Biol 22:1106-15. 2002
    ..These results are consistent with a multimeric structure for Spo11p in vivo but may also indicate that the amount of Spo11 protein is not a limiting factor for DSB formation in normal cells...
  22. ncbi request reprint Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break formation, independent of its cytoplasmic role in RNA metabolism
    Charanjit Arora
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center and Weill Graduate School of Medical Sciences of Cornell University, 1275 York Avenue, Box 97, New York, NY 10021, USA
    Mol Cell 13:549-59. 2004
    ..Ski8 works with Spo11 to recruit other DSB proteins to meiotic chromosomes, implicating Ski8 as a scaffold protein mediating assembly of a multiprotein complex essential for DSB formation...
  23. ncbi request reprint Where the crossovers are: recombination distributions in mammals
    Liisa Kauppi
    Department of Genetics, University of Leicester, Leicester LE1 7RH, UK
    Nat Rev Genet 5:413-24. 2004
  24. pmc Distinct DNA-damage-dependent and -independent responses drive the loss of oocytes in recombination-defective mouse mutants
    Monica Di Giacomo
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center and Weill Graduate School of Medical Sciences of Cornell University, 1275 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 102:737-42. 2005
    ..The absence of ATM caused defects in folliculogenesis that were similar to those in Dmc1 mutants and that could be suppressed by Spo11 mutation, implying that oocyte death in Atm-deficient animals is a response to defective DSB repair...
  25. pmc Meiotic crossover hotspots contained in haplotype block boundaries of the mouse genome
    Liisa Kauppi
    Molecular Biology and Developmental Biology Programs, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 104:13396-401. 2007
    ..As increasing amounts of single-nucleotide polymorphism data emerge, this approach will be useful for investigating the recombination landscape of the mouse genome...
  26. pmc Molecular cartography: mapping the landscape of meiotic recombination
    Jing Pan
    Memorial Sloan Kettering Cancer Center, New York, New York, United States of America
    PLoS Biol 5:e333. 2007
  27. pmc Crossover homeostasis in yeast meiosis
    Emmanuelle Martini
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Cell 126:285-95. 2006
    ..Our results distinguish between existing models of crossover control and support the hypothesis that an obligate crossover is a genetically programmed event tied to crossover interference...
  28. doi request reprint Probing meiotic recombination decisions
    Ignasi Roig
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Dev Cell 15:331-2. 2008
    ..These data provide insights into the molecular "decisions" that control the outcome of the recombination process...
  29. ncbi request reprint Homologous recombination: needing to have my say
    Takashi Okada
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Curr Biol 15:R200-2. 2005
    ....
  30. pmc Cyclin-dependent kinase directly regulates initiation of meiotic recombination
    Kiersten A Henderson
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, and Weill Graduate School of Medical Sciences, Cornell University, New York, NY 10021, USA
    Cell 125:1321-32. 2006
    ..We propose that this function of Cdc28 helps to coordinate the events of meiotic prophase with each other and with progression through prophase...
  31. pmc A role for histone H2B during repair of UV-induced DNA damage in Saccharomyces cerevisiae
    Emmanuelle M D Martini
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Genetics 160:1375-87. 2002
    ..When combined with a ubc13Delta mutation, which is also epistatic with rad5Delta, the htb1-3 mutation enhanced UV-induced cell killing. These results suggest that histone H2B acts in a novel RAD5-dependent branch of PRR...
  32. ncbi request reprint Modifying histones and initiating meiotic recombination; new answers to an old question
    Shohreh Maleki
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, Box 97, New York, NY 10021, USA
    Cell 118:404-6. 2004
    ..Several recent studies, including (this issue of Cell), shed light on this issue by revealing roles for posttranslational histone modifications in promoting DSB formation...
  33. pmc Surveillance of different recombination defects in mouse spermatocytes yields distinct responses despite elimination at an identical developmental stage
    Marco Barchi
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Mol Cell Biol 25:7203-15. 2005
    ..Thus, despite equivalent stages of spermatocyte elimination, different recombination-defective mutants manifest distinct responses, providing insight into surveillance mechanisms in male meiosis...
  34. pmc Tying synaptonemal complex initiation to the formation and programmed repair of DNA double-strand breaks
    Kiersten A Henderson
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center and Weill Graduate School of Medical Sciences, Cornell University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 101:4519-24. 2004
    ..The quantitative relationship between Zip3 foci, SC formation, and crossing over strongly implicates crossover-designated recombination intermediates as the sites of SC initiation...
  35. pmc Genome destabilization by homologous recombination in the germ line
    Mariko Sasaki
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, 10065, USA
    Nat Rev Mol Cell Biol 11:182-95. 2010
    ..Studies in yeast have provided insights into the molecular mechanisms of meiotic NAHR as well as the cellular strategies that limit it...
  36. pmc Detection of SPO11-oligonucleotide complexes from mouse testes
    Jing Pan
    Memorial Sloan Kettering Cancer Center, New York, NY, USA
    Methods Mol Biol 557:197-207. 2009
    ..This chapter describes detection of the release product, SPO11-oligonucleotide complexes, from mouse testis lysates. The method for determining the size of SPO11-associated oligonucleotides is also provided...
  37. pmc Gel electrophoresis assays for analyzing DNA double-strand breaks in Saccharomyces cerevisiae at various spatial resolutions
    Hajime Murakami
    Memorial Sloan Kettering Cancer Center, New York, NY, USA
    Methods Mol Biol 557:117-42. 2009
    ..By varying the type of gel electrophoresis and other parameters, the spatial resolution of DSB analysis can range from single nucleotides up to whole yeast chromosomes...
  38. ncbi request reprint H2B (Ser10) phosphorylation is induced during apoptosis and meiosis in S. cerevisiae
    Sung Hee Ahn
    Laboratory of Chromatin Biology, The Rockefeller University, New York, New York 10021, USA
    Cell Cycle 4:780-3. 2005
    ..How phosphorylation marks are translated into meaningful downstream events during processes as diverse as apoptosis and meiosis remain a challenge for future studies...
  39. ncbi request reprint Clarifying the mechanics of DNA strand exchange in meiotic recombination
    Matthew J Neale
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York 10021, USA
    Nature 442:153-8. 2006
    ....
  40. ncbi request reprint Mice deficient for the type II topoisomerase-like DNA transesterase Spo11 show normal immunoglobulin somatic hypermutation and class switching
    Ulf Klein
    Institute for Cancer Genetics, Columbia University, New York, USA
    Eur J Immunol 32:316-21. 2002
    ..Furthermore, Spo11(-/-) mice showed normal serum levels of all Ig isotypes. These results indicate that Spo11 is not required for Ig hypermutation or class switch recombination...