Jerard Hurwitz

Summary

Affiliation: Memorial Sloan-Kettering Cancer Center
Country: USA

Publications

  1. ncbi request reprint The discovery of RNA polymerase
    Jerard Hurwitz
    Program of Molecular Biology, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 280:42477-85. 2005
  2. ncbi request reprint The influence of the Cdc27 subunit on the properties of the Schizosaccharomyces pombe DNA polymerase delta
    Vladimir P Bermudez
    Memorial Sloan Kettering Cancer Center, Program of Molecular Biology, New York, New York 10021, USA
    J Biol Chem 277:36853-62. 2002
  3. ncbi request reprint Fission yeast Mcm10p contains primase activity
    Karen Fien
    Program of Molecular Biology, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 281:22248-60. 2006
  4. pmc The Cdc23 (Mcm10) protein is required for the phosphorylation of minichromosome maintenance complex by the Dfp1-Hsk1 kinase
    Joon Kyu Lee
    Program in Molecular Biology, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 100:2334-9. 2003
  5. ncbi request reprint Primer utilization by DNA polymerase alpha-primase is influenced by its interaction with Mcm10p
    Karen Fien
    Program in Molecular Biology, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 279:16144-53. 2004
  6. ncbi request reprint His239Arg SNP of HRAD9 is associated with lung adenocarcinoma
    Yoshimasa Maniwa
    Division of Cardiovascular, Thoracic, and Pediatric Surgery, Kobe University Graduate School of Medicine, Kobe, Japan
    Cancer 106:1117-22. 2006
  7. pmc CDC7 kinase phosphorylates serine residues adjacent to acidic amino acids in the minichromosome maintenance 2 protein
    Won Ho Cho
    Department of Biology Education, Seoul National University, Seoul 151 742, Korea
    Proc Natl Acad Sci U S A 103:11521-6. 2006
  8. ncbi request reprint A conserved physical and functional interaction between the cell cycle checkpoint clamp loader and DNA ligase I of eukaryotes
    Wei Song
    Molecular Medicine Graduate Program, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 282:22721-30. 2007
  9. pmc Studies with the human cohesin establishment factor, ChlR1. Association of ChlR1 with Ctf18-RFC and Fen1
    Andrea Farina
    Program of Molecular Biology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA
    J Biol Chem 283:20925-36. 2008
  10. pmc Stimulation of DNA synthesis activity of human DNA polymerase kappa by PCNA
    Lajos Haracska
    Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555 1061, USA
    Mol Cell Biol 22:784-91. 2002

Research Grants

  1. Studies on the Checkpoint Regulation of DNA Synthesis
    Jerard Hurwitz; Fiscal Year: 2005
  2. STUDIES ON EUKARYOTIC DNA REPLICATION
    Jerard Hurwitz; Fiscal Year: 2007
  3. The role of establishment factors in cohesion
    Jerard Hurwitz; Fiscal Year: 2007
  4. Studies on Eukaryotic Replication
    Jerard Hurwitz; Fiscal Year: 2009
  5. The role of establishment factors in cohesion
    Jerard Hurwitz; Fiscal Year: 2009
  6. Studies on Eukaryotic Replication
    Jerard Hurwitz; Fiscal Year: 2010
  7. The role of establishment factors in cohesion
    Jerard Hurwitz; Fiscal Year: 2010
  8. STUDIES ON EUKARYOTIC DNA REPLICATION
    Jerard Hurwitz; Fiscal Year: 2003
  9. EUKARYOTIC DNA REPLICATION
    Jerard Hurwitz; Fiscal Year: 1999
  10. S POMBE PROTEIN/CENTROMERIC DNA INTERACTION
    Jerard Hurwitz; Fiscal Year: 2001

Collaborators

  • Katsunori Tanaka
  • Maria E Gaczynska
  • Shiv Is Grewal
  • Robin Allshire
  • Jun Ichi Nakayama
  • Karen Fien
  • Vladimir P Bermudez
  • Yoshimasa Maniwa
  • Joon Kyu Lee
  • Zvi Kelman
  • Tatsuro S Takahashi
  • Andrea Farina
  • Wei Song
  • Vladimir Bermudez
  • Won Ho Cho
  • Yutaka Okita
  • Chiho Ohbayashi
  • Naoki Kanomata
  • Kenji Okada
  • Yeon Soo Seo
  • Masahiro Yoshimura
  • Yoshitake Hayashi
  • Stefan Gazdoiu
  • Inger Tappin
  • Hiromi Nakagawa
  • Lajos Haracska
  • Jae Ho Shin
  • Do Hyung Kim
  • Johannes C Walter
  • Abhijit Basu
  • Johnson Varkey
  • Sean Post
  • David S Levin
  • Alan E Tomkinson
  • Yoshihiro Nishimura
  • Young Joo Lee
  • Soo Im Kong
  • Kosj Yamoah
  • Kenneth Wu
  • Aneel K Aggarwal
  • Carlos R Escalante
  • Zhen Qiang Pan
  • Takeshi Yuki
  • Anthony J Cesare
  • Aziz Sancar
  • Laura A Lindsey-Boltz
  • Jack D Griffith
  • Louise Prakash
  • Satya Prakash
  • Barbara B Phillips
  • Ildiko Unk
  • Robert E Johnson
  • Yota Murakami
  • Stuart A MacNeill

Detail Information

Publications17

  1. ncbi request reprint The discovery of RNA polymerase
    Jerard Hurwitz
    Program of Molecular Biology, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 280:42477-85. 2005
  2. ncbi request reprint The influence of the Cdc27 subunit on the properties of the Schizosaccharomyces pombe DNA polymerase delta
    Vladimir P Bermudez
    Memorial Sloan Kettering Cancer Center, Program of Molecular Biology, New York, New York 10021, USA
    J Biol Chem 277:36853-62. 2002
    ..In vivo, the introduction of these fused Cdc27 derivatives into cdc27Delta cells conferred viability. These data support the model in which Cdc27 plays an essential role in DNA replication by recruiting PCNA to the pol delta holoenzyme...
  3. ncbi request reprint Fission yeast Mcm10p contains primase activity
    Karen Fien
    Program of Molecular Biology, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 281:22248-60. 2006
    ..A point mutant within this motif of S. pombe Mcm10p was defective in primer synthesis in vitro, and this mutant failed to support growth in vivo, suggesting that the primase activity of Mcm10p may be essential for cell viability...
  4. pmc The Cdc23 (Mcm10) protein is required for the phosphorylation of minichromosome maintenance complex by the Dfp1-Hsk1 kinase
    Joon Kyu Lee
    Program in Molecular Biology, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 100:2334-9. 2003
    ..These results suggest that Cdc23p participates in the activation of prereplicative complex by recruiting the Dfp1-Hsk1 kinase and stimulating the phosphorylation of the Mcm complex...
  5. ncbi request reprint Primer utilization by DNA polymerase alpha-primase is influenced by its interaction with Mcm10p
    Karen Fien
    Program in Molecular Biology, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 279:16144-53. 2004
    ..We suggest that the interaction of Mcm10p with the pol alpha-primase complex, its binding to single-stranded DNA, and its activation of the polymerase complex together contribute to its role in the elongation phase of DNA replication...
  6. ncbi request reprint His239Arg SNP of HRAD9 is associated with lung adenocarcinoma
    Yoshimasa Maniwa
    Division of Cardiovascular, Thoracic, and Pediatric Surgery, Kobe University Graduate School of Medicine, Kobe, Japan
    Cancer 106:1117-22. 2006
    ..Those experiments, however, did not examine whether the hRad9 gene was mutated in those cells. The sequence of the HRAD9 gene in NSCLC cells was investigated...
  7. pmc CDC7 kinase phosphorylates serine residues adjacent to acidic amino acids in the minichromosome maintenance 2 protein
    Won Ho Cho
    Department of Biology Education, Seoul National University, Seoul 151 742, Korea
    Proc Natl Acad Sci U S A 103:11521-6. 2006
    ..This finding may explain why the sequential action of cyclin-dependent and Cdc7 kinases is essential for the initiation of DNA replication...
  8. ncbi request reprint A conserved physical and functional interaction between the cell cycle checkpoint clamp loader and DNA ligase I of eukaryotes
    Wei Song
    Molecular Medicine Graduate Program, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 282:22721-30. 2007
    ..Thus, our results suggest that the in vivo interaction between DNA ligase I and the checkpoint clamp loader is regulated by post-translational modification of DNA ligase I...
  9. pmc Studies with the human cohesin establishment factor, ChlR1. Association of ChlR1 with Ctf18-RFC and Fen1
    Andrea Farina
    Program of Molecular Biology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA
    J Biol Chem 283:20925-36. 2008
    ..These findings are consistent with a role of hChlR1 in the establishment of sister chromatid cohesion and suggest that its action may contribute to lagging strand processing events important in cohesion...
  10. pmc Stimulation of DNA synthesis activity of human DNA polymerase kappa by PCNA
    Lajos Haracska
    Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555 1061, USA
    Mol Cell Biol 22:784-91. 2002
    ....
  11. pmc Proximity-induced activation of human Cdc34 through heterologous dimerization
    Stefan Gazdoiu
    Department of Oncological Sciences, Mount Sinai School of Medicine, New York, NY 10029 6574, USA
    Proc Natl Acad Sci U S A 102:15053-8. 2005
    ..Thus, juxtaposition of human Cdc34 activates its catalytic capability, suggesting that the SCF-mediated polyubiquitination reaction may require the conversion of Cdc34 from an inactive monomer to a highly active dimeric form...
  12. pmc Atomic force microscopic analysis of the binding of the Schizosaccharomyces pombe origin recognition complex and the spOrc4 protein with origin DNA
    Maria Gaczynska
    Institute of Biotechnology, University of Texas Health Science Center, 15355 Lambda Drive, San Antonio, TX 78245, USA
    Proc Natl Acad Sci U S A 101:17952-7. 2004
    ..These include the selective binding of the complex to A- and T-rich regions and the alteration of the DNA structure due to its wrapping around spOrc4p...
  13. ncbi request reprint Accumulation of hRad9 protein in the nuclei of nonsmall cell lung carcinoma cells
    Yoshimasa Maniwa
    Division of Cardiovascular, Thoracic, and Pediatric Surgery, Kobe University Graduate School of Medicine, Kobe, Japan
    Cancer 103:126-32. 2005
    ..Deficiencies in these proteins are directly linked to the accumulation of gene mutations, which can induce cellular transformation and result in malignant disease...
  14. ncbi request reprint Structural lessons in DNA replication from the third domain of life
    Zvi Kelman
    Nat Struct Biol 10:148-50. 2003
  15. pmc Loading of the human 9-1-1 checkpoint complex onto DNA by the checkpoint clamp loader hRad17-replication factor C complex in vitro
    Vladimir P Bermudez
    Program in Molecular Biology, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 100:1633-8. 2003
    ..Rad17-RFC binds to nicked circular, gapped, and primed DNA and recruits the 9-1-1 complex in an ATP-dependent manner. Electron microscopic analyses of the reaction products indicate that the 9-1-1 ring is clamped around the DNA...
  16. pmc Fission yeast CENP-B homologs nucleate centromeric heterochromatin by promoting heterochromatin-specific histone tail modifications
    Hiromi Nakagawa
    Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    Genes Dev 16:1766-78. 2002
    ..These results indicate that the CENP-B homologs act as site-specific nucleation factors for the formation of centromeric heterochromatin by heterochromatin-specific modifications of histone tails...
  17. pmc Cdc7-Drf1 kinase links chromosome cohesion to the initiation of DNA replication in Xenopus egg extracts
    Tatsuro S Takahashi
    Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 22:1894-905. 2008
    ..These results show that DDK is required to tether Scc2-Scc4 to pre-RCs, and they underscore the intimate link between early steps in DNA replication and cohesion...

Research Grants30

  1. Studies on the Checkpoint Regulation of DNA Synthesis
    Jerard Hurwitz; Fiscal Year: 2005
    ..Cid1 is a homologue of the S. serevisiae Trf4 (Trf5) which was recently shown to possess beta-like DNA polymerase activity. ..
  2. STUDIES ON EUKARYOTIC DNA REPLICATION
    Jerard Hurwitz; Fiscal Year: 2007
    ....
  3. The role of establishment factors in cohesion
    Jerard Hurwitz; Fiscal Year: 2007
    ....
  4. Studies on Eukaryotic Replication
    Jerard Hurwitz; Fiscal Year: 2009
    ..We plan to isolate the proteins involved in copying DNA and determine how they function. Results from these studies will have a direct impact on genetic diseases and cancer. ..
  5. The role of establishment factors in cohesion
    Jerard Hurwitz; Fiscal Year: 2009
    ....
  6. Studies on Eukaryotic Replication
    Jerard Hurwitz; Fiscal Year: 2010
    ..We plan to isolate the proteins involved in copying DNA and determine how they function. Results from these studies will have a direct impact on genetic diseases and cancer. ..
  7. The role of establishment factors in cohesion
    Jerard Hurwitz; Fiscal Year: 2010
    ....
  8. STUDIES ON EUKARYOTIC DNA REPLICATION
    Jerard Hurwitz; Fiscal Year: 2003
    ..abstract_text> ..
  9. EUKARYOTIC DNA REPLICATION
    Jerard Hurwitz; Fiscal Year: 1999
    ..We plan to isolate the ORC polypeptides from S. pombe and to further characterize two distinct protein isolated from S. pombe that interact with the S. pombe conserved core sequence. ..
  10. S POMBE PROTEIN/CENTROMERIC DNA INTERACTION
    Jerard Hurwitz; Fiscal Year: 2001
    ..pombe centromeric DNA sequences, determine their nuclear localization, utilize them as means to identify other centromere proteins and examine their role in centromeric DNA transactions and functions. ..
  11. Studies on Eukaryotic Replication
    Jerard Hurwitz; Fiscal Year: 2009
    ..We plan to isolate the proteins involved in copying DNA and determine how they function. Results from these studies will have a direct impact on genetic diseases and cancer. ..