W Hu

Summary

Affiliation: Memorial Sloan-Kettering Cancer Center
Country: USA

Publications

  1. ncbi Sensitivity enhanced NMR spectroscopy by quenching scalar coupling mediated relaxation: application to the direct observation of hydrogen bonds in 13C/15N-labeled proteins
    A Liu
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    J Biomol NMR 17:55-61. 2000
  2. ncbi Detection of very weak side chain-main chain hydrogen bonding interactions in medium-size 13C/15N-labeled proteins by sensitivity-enhanced NMR spectroscopy
    A Liu
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    J Biomol NMR 17:79-82. 2000
  3. ncbi NMR detection of side chain-side chain hydrogen bonding interactions in 13C/15N-labeled proteins
    A Liu
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    J Biomol NMR 17:305-10. 2000
  4. ncbi Sensitivity-enhanced MQ-HCN-CCH-TOCSY and MQ-HCN-CCH-COSY pulse schemes for (13)C/(15)N labeled RNA oligonucleotides
    W Hu
    Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York, 10021, USA
    J Magn Reson 145:147-51. 2000
  5. ncbi Novel 2D and 3D multiple-quantum bi-directional HCNCH experiments for the correlation of ribose and base protons/carbons in 13C/15N labeled RNA
    W Hu
    Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    J Biomol NMR 20:167-72. 2001
  6. ncbi Multiple-quantum HCN-CCH-TOCSY experiment for 13C/15N labeled RNA oligonucleotides
    W Hu
    Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    J Biomol NMR 15:289-93. 1999
  7. ncbi Peptide-triggered conformational switch in HIV-1 RRE RNA complexes
    Y Gosser
    Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA
    Nat Struct Biol 8:146-50. 2001
  8. ncbi Determination of 3J(H3í, Pi+1) and 3J(H5í/5i, Pi) coupling constants in 13C-labeled nucleic acids using constant-time HMQC
    W Hu
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York 10021, USA
    J Magn Reson 139:181-5. 1999

Collaborators

Detail Information

Publications8

  1. ncbi Sensitivity enhanced NMR spectroscopy by quenching scalar coupling mediated relaxation: application to the direct observation of hydrogen bonds in 13C/15N-labeled proteins
    A Liu
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    J Biomol NMR 17:55-61. 2000
    ..The experimental data fit theoretical analysis well. The significant enhancement in sensitivity makes the experiment more applicable to larger-sized proteins without resorting to perdeuteration...
  2. ncbi Detection of very weak side chain-main chain hydrogen bonding interactions in medium-size 13C/15N-labeled proteins by sensitivity-enhanced NMR spectroscopy
    A Liu
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    J Biomol NMR 17:79-82. 2000
    ..The 3hJ(NCO2delta) coupling constant appears to be even smaller than the average value of backbone 3hJ(NC') couplings, consistent with more extensive local dynamics in protein side chains...
  3. ncbi NMR detection of side chain-side chain hydrogen bonding interactions in 13C/15N-labeled proteins
    A Liu
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    J Biomol NMR 17:305-10. 2000
    ....
  4. ncbi Sensitivity-enhanced MQ-HCN-CCH-TOCSY and MQ-HCN-CCH-COSY pulse schemes for (13)C/(15)N labeled RNA oligonucleotides
    W Hu
    Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York, 10021, USA
    J Magn Reson 145:147-51. 2000
    ..The pulse schemes have been demonstrated on a 23-mer (13)C/(15)N-labeled RNA aptamer complexed with neomycin and tested on a 32-mer RNA complexed with a 23-residue peptide...
  5. ncbi Novel 2D and 3D multiple-quantum bi-directional HCNCH experiments for the correlation of ribose and base protons/carbons in 13C/15N labeled RNA
    W Hu
    Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    J Biomol NMR 20:167-72. 2001
    ..Both experiments were tested on a 13C/15N labeled 27-mer HIV-1 TAR RNA containing a UUCG hairpin loop...
  6. ncbi Multiple-quantum HCN-CCH-TOCSY experiment for 13C/15N labeled RNA oligonucleotides
    W Hu
    Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    J Biomol NMR 15:289-93. 1999
    ..Compared to the single-quantum version, the sensitivity of the multiple-quantum HCN-CCH-TOCSY experiment is enhanced on average by a factor of 2 for a 23-mer RNA aptamer complexed with neomycin...
  7. ncbi Peptide-triggered conformational switch in HIV-1 RRE RNA complexes
    Y Gosser
    Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA
    Nat Struct Biol 8:146-50. 2001
    ..The conformational switch has been visualized by NMR chemical shift mapping of the uridine H5/H6 atoms during a competition experiment in which Rev peptide was displaced from RRE-IIB by the higher affinity binding selected peptide...
  8. ncbi Determination of 3J(H3í, Pi+1) and 3J(H5í/5i, Pi) coupling constants in 13C-labeled nucleic acids using constant-time HMQC
    W Hu
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York 10021, USA
    J Magn Reson 139:181-5. 1999
    ..The introduction of beta and epsilon restraints has improved the convergence as well as the quality of d(GGAGGAT) structure...