Research Topics
Species | Stuart M ChambersSummaryAffiliation: Memorial Sloan-Kettering Cancer Center Country: USA Publications
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Detail Information
Publications
Combined small-molecule inhibition accelerates developmental timing and converts human pluripotent stem cells into nociceptorsStuart M Chambers
Center for Stem Cell Biology, Sloan Kettering Institute, New York, New York, USA
Nat Biotechnol 30:715-20. 2012..The quick and high-efficiency derivation of nociceptors offers unprecedented access to this medically relevant cell type for studies of human pain...
Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signalingStuart M Chambers
Developmental Biology Program, Sloan Kettering Institute, 1275 York Ave, New York, New York 10065, USA
Nat Biotechnol 27:275-80. 2009..Noggin/SB431542-based neural induction should facilitate the use of hES and hiPS cells in regenerative medicine and disease modeling and obviate the need for protocols based on stromal feeders or embryoid bodies...
Cell fate plug and play: direct reprogramming and induced pluripotencyStuart M Chambers
Center for Stem Cell Biology, Sloan Kettering Institute, 1275 York Avenue, New York, NY 10065, USA
Cell 145:827-30. 2011..Here we discuss the advantages and challenges of harnessing this direct reprogramming method for regenerative medicine...
Modelling pathogenesis and treatment of familial dysautonomia using patient-specific iPSCsGabsang Lee
Developmental Biology Program, Sloan Kettering Institute, 1275 York Ave, USA
Nature 461:402-6. 2009..Our study illustrates the promise of iPSC technology for gaining new insights into human disease pathogenesis and treatment...
Derivation of neural crest cells from human pluripotent stem cellsGabsang Lee
Developmental Biology Program, Sloan Kettering Institute, New York, NY, USA
Nat Protoc 5:688-701. 2010..Our protocol has been validated in multiple independent hESC and hiPSC lines. The average time required for generating purified hPSC-NC precursors using this protocol is 2-5 weeks...
Efficient derivation of functional floor plate tissue from human embryonic stem cellsChristopher A Fasano
Developmental Biology Program, Sloan Kettering Institute, 1275 York Ave, New York, NY 10065, USA
Cell Stem Cell 6:336-47. 2010..These data define the early signals that drive human FP versus AN specification and determine regional identity in hESC-derived FP...
ZFX controls the self-renewal of human embryonic stem cellsSivan Harel
Department of Microbiology and Immunology, Columbia University Medical Center, New York, New York, United States of America
PLoS ONE 7:e42302. 2012..Thus, ZFX acts as a molecular rheostat regulating the balance between self-renewal and differentiation in hESCs, revealing the close evolutionary conservation of the self-renewal mechanisms in murine and human ESCs...
Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiationEirini P Papapetrou
Center for Cell Engineering, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
Proc Natl Acad Sci U S A 106:12759-64. 2009..The vector system described here presents a powerful tool for mechanistic studies of reprogramming and the optimization of hiPSC generation...
Converting human pluripotent stem cells to neural tissue and neurons to model neurodegenerationStuart M Chambers
Developmental Biology Program, Sloan Kettering Institute, New York, NY, USA
Methods Mol Biol 793:87-97. 2011..In this chapter, we detail methods to direct hESCs or hiPSCs into early neural cells and subsequently postmitotic neurons...
