Research Topics
Genomes and Genes | John J WoodwardSummaryAffiliation: Medical University of South Carolina Country: USA Publications
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Publications
Ethanol inhibition of up-states in prefrontal cortical neurons expressing the genetically encoded calcium indicator GCaMP3John J Woodward
Department of Neurosciences, Medical University of South Carolina, Charleston, 29425, USA
Alcohol Clin Exp Res 36:780-7. 2012..In the present study, we used imaging and a genetically encoded calcium sensor to examine the effects of EtOH on the activity of multiple neurons simultaneously during up-states...
Prostacyclin-induced rundown of N-methyl-D-aspartate receptor currents in HEK293 cells is protein kinase A-dependent and NR2 subunit-selectiveJohn J Woodward
Department of Physiology and Neuroscience, MUSC, Charleston, USA
J Neurochem 80:598-604. 2002..These results suggest that PKA influences the functional activity of NMDA receptors in an NR2 subunit-selective fashion...- Pharmacological characterization of glycine-activated currents in HEK 293 cells expressing N-methyl-D-aspartate NR1 and NR3 subunitsC Thetford Smothers
Department of Neurosciences, Division of Neuroscience Research, and Center for Drug and Alcohol Programs, Medical University of South Carolina, Charleston, South Carolina 29425, USA
J Pharmacol Exp Ther 322:739-48. 2007..The results of this study indicate that coexpression of NR1, NR3A, and NR3B subunits in HEK 293 cells results in glycineactivated receptors with novel functional and pharmacological properties... - Fyn kinase does not reduce ethanol inhibition of zinc-insensitive NR2A-containing N-methyl-D-aspartate receptorsJohn J Woodward
Department of Physiology and Neuroscience and Center for Drug and Alcohol Programs, 173 Ashley Avenue, Suite 403, Medical University of South Carolina, Charleston, SC 29425, USA
Alcohol 34:101-5. 2004..Together, results of the current study indicate that Fyn kinase does not directly affect the ethanol sensitivity of NR1/2A receptors... - GABAA alpha 4 receptor subunits and ethanol: a knockout punch?John J Woodward
Department of Neurosciences and Center for Drug and Alcohol Programs, Medical University of South Carolina, Charleston, South Carolina 29425, USA
Alcohol Clin Exp Res 32:8-9. 2008 - From blue states to up states: a regional view of NMDA-ethanol interactionsJohn J Woodward
Department of Neurosciences and Center for Drug and Alcohol Programs, Medical University of South Carolina, Charleston, South Carolina 29425, USA
Alcohol Clin Exp Res 30:359-67. 2006..Finally, John Woodward described the effects of ethanol on the activity of neurons in deep layers of the prefrontal cortex using a novel slice coculture preparation... - Effects of the abused solvent toluene on recombinant P2X receptors expressed in HEK293 cellsJohn J Woodward
Department of Psychiatry and Neuroscience, Center for Drug and Alcohol Programs, Medical University of South Carolina, Charleston 29425, USA
Brain Res Mol Brain Res 125:86-95. 2004..The results of this study suggest that the abused solvent toluene affects the function of P2X receptors in a subunit-dependent and agonist-dependent fashion... - Ethanol inhibition of recombinant NR1/2A receptors: effects of heavy metal chelators and a zinc-insensitive NR2A mutantJohn J Woodward
Department of Physiology and Neuroscience and Center for Drug and Alcohol Programs, Medical University of South Carolina, Charleston, SC 29425, USA
Alcohol 31:71-6. 2003..These results support the suggestion that low levels of zinc present in experimental solutions may affect the apparent ethanol sensitivity of NMDA receptors containing the NR2A subunit... - Effects of ethanol on persistent activity and up-States in excitatory and inhibitory neurons in prefrontal cortexJohn J Woodward
Department of Neurosciences and Center for Drug and Alcohol Programs, Medical University of South Carolina, Charleston, SC 29425, USA
Alcohol Clin Exp Res 33:2134-40. 2009..In the present study, we examine the effect of ethanol on the activity of fast-spiking (FS) interneurons, a sub-population of neurons critically involved in shaping cortical activity... - Small conductance calcium-activated potassium type 2 channels regulate alcohol-associated plasticity of glutamatergic synapsesPatrick J Mulholland
Department of Neurosciences, Medical University of South Carolina, Charleston, South Carolina 29425 2508, USA
Biol Psychiatry 69:625-32. 2011.... - Glutamate transporters regulate extrasynaptic NMDA receptor modulation of Kv2.1 potassium channelsPatrick J Mulholland
Department of Neurosciences and Center for Drug and Alcohol Programs, Medical University of South Carolina, Charleston, South Carolina 29425, USA
J Neurosci 28:8801-9. 2008..These data support a novel mechanism for glutamate transporters in regulation of neuronal excitability and plasticity through extrasynaptic NMDA receptor modulation of Kv2.1 channels... - Calpeptin provides functional neuroprotection to rat retinal ganglion cells following Ca2+ influxArabinda Das
Department of Neurosciences, Medical University of South Carolina (MUSC, 96 Jonathan Lucas Street, Suite 323K, P.O. Box 250606, Charleston, SC 29425, USA
Brain Res 1084:146-57. 2006..Taken together, our results demonstrated that Ca2+ overload could be responsible for activation of calpain and caspase cascades leading to apoptotic death of RGC-5 cells and CP provided functional neuroprotection... - Enhanced ethanol inhibition of recombinant N-methyl-D-aspartate receptors by magnesium: role of NR3A subunitsChun Jin
Department of Neurosciences and Center for Drug and Alcohol Programs, Medical University of South Carolina, Charleston, South Carolina
Alcohol Clin Exp Res 32:1059-66. 2008.... - Ethanol inhibition of recombinant NMDA receptors is not altered by coexpression of CaMKII-alpha or CaMKII-betaMinfu Xu
Department of Neurosciences and Center for Drug and Alcohol Programs, Medical University of South Carolina, Charleston, SC, USA
Alcohol 42:425-32. 2008..The results of this study suggest that CaMKII is not involved in regulating the acute ethanol sensitivity of NMDA receptors... - Chronic ethanol induces synaptic but not extrasynaptic targeting of NMDA receptorsEzekiel P Carpenter-Hyland
Department of Physiology and Neuroscience, Medical University of South Carolina, Charleston, South Carolina 29425, USA
J Neurosci 24:7859-68. 2004.... - Expression of glycine-activated diheteromeric NR1/NR3 receptors in human embryonic kidney 293 cells Is NR1 splice variant-dependentC Thetford Smothers
Department of Neurosciences, Division of Neuroscience Research, and Center for Drug and Alcohol Programs, Medical University of South Carolina, Charleston, South Carolina 29425, USA
J Pharmacol Exp Ther 331:975-84. 2009..These results indicate a prominent role for NR1 splice variants in the functional expression of NR1/NR3 receptors in mammalian cells... - Ethanol disrupts NMDA receptor and astroglial EAAT2 modulation of Kv2.1 potassium channels in hippocampusPatrick J Mulholland
Department of Neurosciences and Center for Drug and Alcohol Programs, Medical University of South Carolina, 67 President Street, Charleston, SC 29425, USA
Alcohol 43:45-50. 2009..These data demonstrate that acute ethanol disrupts changes in Kv2.1 channels that follow NMDA receptor activation and impairs astroglial regulation of the functional coupling between NMDA receptors and Kv2.1 channels... - Estrogen attenuates glutamate-induced cell death by inhibiting Ca2+ influx through L-type voltage-gated Ca2+ channelsEric A Sribnick
Division of Neurology, Department of Neurosciences, Medical University of South Carolina, 96 Jonathan Lucas Street, Charleston, SC 29425, USA
Brain Res 1276:159-70. 2009..1 cells through effects on L-type Ca(2+) channels, inhibiting Ca(2+) influx as well as activation of the pro-apoptotic proteases calpain and caspase-3... - Ethanol inhibits persistent activity in prefrontal cortical neuronsYali Tu
Department of Neurosciences and Center for Drug and Alcohol Programs, Medical University of South Carolina, Charleston, South Carolina 29425, USA
J Neurosci 27:4765-75. 2007..Ethanol-induced alterations in the activity of deep-layer cortical neurons may underlie some of the behavioral effects associated with ethanol intake... - The abused inhalant toluene differentially modulates excitatory and inhibitory synaptic transmission in deep-layer neurons of the medial prefrontal cortexJacob T Beckley
Department of Neurosciences, Medical University of South Carolina, Charleston, SC 29425, USA
Neuropsychopharmacology 36:1531-42. 2011..Together, these results suggest that the effect of toluene on cognitive behaviors may result from its action on inhibitory and excitatory synaptic transmission of PFC neurons... - Ethanol inhibition of NMDA receptors under conditions of altered protein kinase A activityMinfu Xu
Department of Neuroscience and Center for Drug and Alcohol Programs, Medical University of South Carolina, Charleston, South Carolina 29425, USA
J Neurochem 96:1760-7. 2006..These results suggest that PKA does not appear to play a major role in determining the acute ethanol sensitivity of NMDA receptors... - Medial prefrontal cortex inversely regulates toluene-induced changes in markers of synaptic plasticity of mesolimbic dopamine neuronsJacob T Beckley
Department of Neurosciences, and Center for Drug and Alcohol Programs, Department of Psychiatry, Medical University of South Carolina, Charleston, South Carolina 29425
J Neurosci 33:804-13. 2013.... - Effects of amino acid substitutions in transmembrane domains of the NR1 subunit on the ethanol inhibition of recombinant N-methyl-D-aspartate receptorsC Thetford Smothers
Department of Neurosciences, Division of Neuroscience Research and Center for Drug and Alcohol Programs, Medical University of South Carolina, Charleston, South Carolina 29425, USA
Alcohol Clin Exp Res 30:523-30. 2006..CONCLUSIONS: These results indicate that the ethanol sensitivity of NMDA receptors may be regulated by discrete sites within the TM3 and TM4 domains of the NR1 subunit... - Effects of the abused inhalant toluene on ethanol-sensitive potassium channels expressed in oocytesAngelo M Del Re
Department of Neurosciences and Center for Drug and Alcohol Programs, Medical University of South Carolina, 173 Ashley Avenue, Suite 403, Charleston, 29425, USA
Brain Res 1087:75-82. 2006..The results of these studies show that toluene has effects on BK and GirK channels that are opposite to those of ethanol, suggesting that these channels are unlikely to underlie behaviors that these two drugs of abuse share... - Tolerance to cannabinoid-induced behaviors in mice treated chronically with ethanolMatthew J Pava
Department of Neurosciences, Medical University of South Carolina, Charleston, SC, USA
Psychopharmacology (Berl) 219:137-47. 2012..However, these studies have been limited to measures of ataxia and analysis of CB1 expression from whole-brain or hippocampal preparations... - Effects of 8 different NR1 splice variants on the ethanol inhibition of recombinant NMDA receptorsChun Jin
Department of Neurosciences and Center for Drug and Alcohol Programs, Medical University of South Carolina, Charleston, South Carolina 29425, USA
Alcohol Clin Exp Res 30:673-9. 2006..Although results from previous studies suggest that NR2 subunits affect the ethanol sensitivity of NMDA receptors, the role of the NR1 subunit and its multiple splice variants is less well known... - The inhibition of apoptosis by melatonin in VSC4.1 motoneurons exposed to oxidative stress, glutamate excitotoxicity, or TNF-alpha toxicity involves membrane melatonin receptorsArabinda Das
Department of Neurosciences Division of Neurology, Medical University of South Carolina, Charleston, SC 29425, USA
J Pineal Res 48:157-69. 2010..1 motoneuron apoptosis. Results suggest that neuroprotection rendered by melatonin to motoneurons is receptor mediated and melatonin may be an effective neuroprotective agent to attenuate motoneuron death in CNS injuries and diseases... - Ethanol inhibition of constitutively open N-methyl-D-aspartate receptorsMinfu Xu
Department of Neurosciences, Medical University of South Carolina, Charleston, SC 29425, USA
J Pharmacol Exp Ther 340:218-26. 2012..Overall, these results show that ethanol affects NMDA receptor function at a site distal from agonist binding and appears to exert greater effects via perturbation of GluN2 subunits... - A review of the interactions between alcohol and the endocannabinoid system: implications for alcohol dependence and future directions for researchMatthew J Pava
Department of Neurosciences, Medical University of South Carolina, Charleston, 29403, USA
Alcohol 46:185-204. 2012..These results are compiled into a summary providing a scheme for the known changes to the components of the EC system in different stages of alcohol dependence. Finally, future directions for research are discussed... - Effect of the NR3 subunit on ethanol inhibition of recombinant NMDA receptorsC Thetford Smothers
Department of Physiology and Neuroscience, Center for Drug and Alcohol Programs, Medical University of South Carolina, Charleston, SC 29425, USA
Brain Res 987:117-21. 2003..Overall, these results suggest that the NR3A subunit is not a determinant of ethanol sensitivity in recombinant NMDA receptors... - Effects of chronic intermittent ethanol exposure on orbitofrontal and medial prefrontal cortex-dependent behaviors in miceKimberly A Badanich
Center for Drug and Alcohol Programs, Department of Psychiatry and Behavioral Sciences, Medical University of South Carolina, Charleston, SC 29425, USA
Behav Neurosci 125:879-91. 2011..Given that reversal learning requires an intact OFC, these findings support the idea that the OFC may be vulnerable to the cognitive impairing actions of ethanol... - Effects of ethanol on phosphorylation site mutants of recombinant N-methyl-D-aspartate receptorsMinfu Xu
Department of Neurosciences, Medical University of South Carolina, Charleston, SC 29425, USA
Alcohol 45:373-80. 2011..Together with previously published data, these findings suggest that modification of putative phosphorylation sites could contribute to the overall acute ethanol sensitivity of recombinant NMDA receptors. Supported by R37AA009986... - Alcohol and the prefrontal cortexKenneth Abernathy
Department of Neurosciences, Medical University of South Carolina, Charleston, SC 29425, USA
Int Rev Neurobiol 91:289-320. 2010..The chapter concludes with a discussion of unanswered questions and areas needing further investigation... - Inhibition of gap junction currents by the abused solvent tolueneAngelo M Del Re
Department of Physiology and Neuroscience, Center for Drug and Alcohol Programs, 173 Ashley Avenue, P.O. Box 250510, Medical University of South Carolina, Charleston, SC 29425, USA
Drug Alcohol Depend 78:221-4. 2005..57 mM. The results of these studies suggest that volatile solvents including toluene may produce some of their effects by disrupting inter-cellular communication mediated by gap junction proteins... - Calpain activation in apoptosis of ventral spinal cord 4.1 (VSC4.1) motoneurons exposed to glutamate: calpain inhibition provides functional neuroprotectionArabinda Das
Department of Neurology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
J Neurosci Res 81:551-62. 2005..Inhibition of the activities of both NMDA and AMPA receptors protected VSC4.1 cells from glutamate toxicity and preserved whole-cell membrane potential... - Ethanol selectively attenuates NMDAR-mediated synaptic transmission in the prefrontal cortexCarl Weitlauf
Department of Neurosciences and Center for Drug and Alcohol Programs, Medical University of South Carolina, Charleston, South Carolina 29425, USA
Alcohol Clin Exp Res 32:690-8. 2008..In a previous study from this laboratory, we showed that ethanol inhibits complex patterns of persistent activity (known as "up-states") in medial PFC (mPFC) neurons in a reversible and concentration-dependent manner... - Dephosphorylation of GluN2B C-terminal tyrosine residues does not contribute to acute ethanol inhibition of recombinant NMDA receptorsBenjamin A Hughes
Department of Neurosciences and Center for Drug and Alcohol Programs, Medical University of South Carolina, Charleston, SC 29425, USA
Alcohol 47:181-6. 2013..These findings suggest that dephosphorylation of C-terminal tyrosine residues does not account for ethanol inhibition of GluN2B receptors... - Inhibition of neuronal nicotinic acetylcholine receptors by the abused solvent, tolueneAmbuja S Bale
Department of Pharmacology and Toxicology, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, Virginia, VA 23298, USA
Br J Pharmacol 137:375-83. 2002..5 Taken together, these results suggest that nicotinic receptors, like NMDA receptors, show a subunit-dependent sensitivity to toluene and may represent an important site of action for some of the neurobehavioural effects of toluene... - Toluene inhibits voltage-sensitive calcium channels expressed in pheochromocytoma cellsRiddick Tillar
Department of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, VA 23298, USA
Neurochem Int 41:391-7. 2002..These results show that dihydropyridine sensitive and insensitive calcium channels are inhibited by toluene and may represent important sites of action for this compound... - Ethanol differentially affects ATP-gated P2X(3) and P2X(4) receptor subtypes expressed in Xenopus oocytesDaryl L Davies
Department of Molecular Pharmacology Toxicology, School of Pharmacy, University of Southern California, 1985 Zonal Avenue, Los Angeles, CA 90033, USA
Neuropharmacology 49:243-53. 2005.... - Perturbation of voltage-sensitive Ca2+ channel function by volatile organic solventsTimothy J Shafer
Neurotoxicology Division, MD B105 05, NHEERL, ORD, U S Environmental Protection Agency, Research Triangle Park, NC 27711, USA
J Pharmacol Exp Ther 315:1109-18. 2005..These data demonstrate that VOCs perturb voltage-sensitive Ca2+ channel function in neurons, an effect that could contribute to the acute neurotoxicity of these compounds... - Effects of ethanol on adenosine 5'-triphosphate-gated purinergic and 5-hydroxytryptamine receptorsDaryl L Davies
University of Southern California, Los Angeles, California, USA
Alcohol Clin Exp Res 30:349-58. 2006..Collectively, the presentations provided strong evidence that P2X and 5-HT3 receptors are important targets for ethanol action... - Effects of anesthetics on mutant N-methyl-D-aspartate receptors expressed in Xenopus oocytesJunichi Ogata
University of Texas, Waggoner Center for Alcohol and Addiction Research, 1 University Station A4800, Austin, TX 78712-0159, USA, and Department of Anesthesia, Kyoto University Hospital, Japan
J Pharmacol Exp Ther 318:434-43. 2006..Thus, amino acids in TM3 and TM4 are important for the actions of many anesthetics, but nitrous oxide, benzene, and ketamine seem to have distinct mechanisms for inhibition of the NMDA receptors...
Research Grants
- ETHANOL SENSITIVITY OF NATIVE AND CLONED NMDA RECEPTORSJohn Woodward; Fiscal Year: 2009..Aim 3 will express ethanol-insensitive receptors identified in Aims 1 and 2 in neurons in vitro and in vivo to address which effects of ethanol are mediated by inhibition of NMDA receptors. ..
- Neural Actions of TolueneJohn Woodward; Fiscal Year: 2006..Results obtained from these studies outlined are expected to greatly expand our knowledge of the cellular and molecular targets of these important drugs of abuse. ..
- Ethanol sensitivity--native /recombinant NMDA receptorsJohn Woodward; Fiscal Year: 2006..The successful completion of this training plan will substantially enhance the PI's research expertise and his ability to fulfill the goals of his research. ..
- Training in Alcohol ResearchJohn Woodward; Fiscal Year: 2007..The overall goal of this training is to provide basic and clinical scientists with the skills necessary to develop into independent investigators focused on solving the problems associated with alcoholism and alcohol abuse. ..
- Neural Actions of TolueneJohn J Woodward; Fiscal Year: 2010..Research to be carried out in this proposal will provide a detailed analysis of the effects of abused inhalants on neurons within brain areas involved in addiction. ..
- ETHANOL SENSITIVITY OF NATIVE AND CLONED NMDA RECEPTORSJohn Woodward; Fiscal Year: 2003..Both molecular and pharmacological approaches will be used to unravel the complex interplay between receptors and intracellular signaling processes that ultimately determine the ethanol sensitivity of NMDA receptors. ..