R Clinton Webb

Summary

Affiliation: Medical College of Georgia
Country: USA

Publications

  1. ncbi Molecular aspects of arterial smooth muscle contraction: focus on Rho
    Rob H P Hilgers
    Department of Physiology, Medical College of Georgia, Augusta, GA 30912 3000, USA
    Exp Biol Med (Maywood) 230:829-35. 2005
  2. ncbi Interleukin-10 attenuates vascular responses to endothelin-1 via effects on ERK1/2-dependent pathway
    Fernanda R C Giachini
    Department of Physiology, Medical College of Georgia, Augusta, GA 30912 3000, USA
    Am J Physiol Heart Circ Physiol 296:H489-96. 2009
  3. ncbi Extracellular signal-regulated kinase 1/2 activation, via downregulation of mitogen-activated protein kinase phosphatase 1, mediates sex differences in desoxycorticosterone acetate-salt hypertension vascular reactivity
    Fernanda R Giachini
    Medical College of Georgia, Department of Physiology, 1120 Fifteenth St, CA 2095, Augusta, GA 30912 3000, USA
    Hypertension 55:172-9. 2010
  4. ncbi Expression and functional role of the RhoA/Rho-kinase pathway in rat coeliac artery
    Cleber E Teixeira
    Department of Physiology, Medical College of Georgia, Augusta, Georgia 30912 3000, USA
    Clin Exp Pharmacol Physiol 32:817-24. 2005
  5. ncbi Vasorelaxing effect of BAY 41-2272 in rat basilar artery: involvement of cGMP-dependent and independent mechanisms
    Cleber E Teixeira
    Department of Physiology, Medical College of Georgia, Augusta, GA 30912 3000, USA
    Hypertension 47:596-602. 2006
  6. ncbi Increased activation of stromal interaction molecule-1/Orai-1 in aorta from hypertensive rats: a novel insight into vascular dysfunction
    Fernanda R C Giachini
    Medical College of Georgia, Department of Physiology, 1120 15th St, CA 3141, Augusta, GA 30912 3000, USA
    Hypertension 53:409-16. 2009
  7. ncbi O-GlcNAcylation contributes to augmented vascular reactivity induced by endothelin 1
    Victor V Lima
    Medical College of Georgia, Department of Physiology, 1120 Fifteenth St, CA 3141, Augusta, GA 30912 3000, USA
    Hypertension 55:180-8. 2010
  8. ncbi STIM1/Orai1 contributes to sex differences in vascular responses to calcium in spontaneously hypertensive rats
    Fernanda R C Giachini
    Department of Physiology, Georgia Health Sciences University, Augusta, GA, USA
    Clin Sci (Lond) 122:215-26. 2012
  9. ncbi Thioredoxin reductase inhibition reduces relaxation by increasing oxidative stress and s-nitrosylation in mouse aorta
    Hyehun Choi
    Department of Physiology, Georgia Health Sciences University, Augusta, GA 30912 3000, USA
    J Cardiovasc Pharmacol 58:522-7. 2011
  10. ncbi Interleukin-10 inhibits the in vivo and in vitro adverse effects of TNF-alpha on the endothelium of murine aorta
    Saiprasad M Zemse
    Department of Physiology, Medical College of Georgia, Augusta, GA 30912, USA
    Am J Physiol Heart Circ Physiol 299:H1160-7. 2010

Detail Information

Publications100

  1. ncbi Molecular aspects of arterial smooth muscle contraction: focus on Rho
    Rob H P Hilgers
    Department of Physiology, Medical College of Georgia, Augusta, GA 30912 3000, USA
    Exp Biol Med (Maywood) 230:829-35. 2005
    ..Removal of Ca(2+) from the cytosol and stimulation of myoson phosphatase initiate the relaxation of vascular smooth muscle...
  2. ncbi Interleukin-10 attenuates vascular responses to endothelin-1 via effects on ERK1/2-dependent pathway
    Fernanda R C Giachini
    Department of Physiology, Medical College of Georgia, Augusta, GA 30912 3000, USA
    Am J Physiol Heart Circ Physiol 296:H489-96. 2009
    ..These data demonstrate that IL-10 counteracts ET(A)-mediated vascular responses to ET-1, as well as activation of the ERK1/2 pathway...
  3. ncbi Extracellular signal-regulated kinase 1/2 activation, via downregulation of mitogen-activated protein kinase phosphatase 1, mediates sex differences in desoxycorticosterone acetate-salt hypertension vascular reactivity
    Fernanda R Giachini
    Medical College of Georgia, Department of Physiology, 1120 Fifteenth St, CA 2095, Augusta, GA 30912 3000, USA
    Hypertension 55:172-9. 2010
    ..In addition, mitogen-activated protein kinase phosphatase 1 and interleukin 10 play regulatory roles in this process...
  4. ncbi Expression and functional role of the RhoA/Rho-kinase pathway in rat coeliac artery
    Cleber E Teixeira
    Department of Physiology, Medical College of Georgia, Augusta, Georgia 30912 3000, USA
    Clin Exp Pharmacol Physiol 32:817-24. 2005
    ..The findings of the present study suggest that endogenous nitric oxide-induced relaxation is mediated, in part, by inhibition of RhoA/ROK signalling in this tissue...
  5. ncbi Vasorelaxing effect of BAY 41-2272 in rat basilar artery: involvement of cGMP-dependent and independent mechanisms
    Cleber E Teixeira
    Department of Physiology, Medical College of Georgia, Augusta, GA 30912 3000, USA
    Hypertension 47:596-602. 2006
    ..BAY 41-2272 potently relaxes the rat basilar artery in a synergistic fashion with NO. Targeting the sGC with selective activators, such as BAY 41-2272, may represent a new therapy to treat cerebrovascular disease...
  6. ncbi Increased activation of stromal interaction molecule-1/Orai-1 in aorta from hypertensive rats: a novel insight into vascular dysfunction
    Fernanda R C Giachini
    Medical College of Georgia, Department of Physiology, 1120 15th St, CA 3141, Augusta, GA 30912 3000, USA
    Hypertension 53:409-16. 2009
    ..Augmented activation of STIM-1/Orai-1 may represent the mechanism that leads to impaired control of intracellular Ca(2+) levels in hypertension...
  7. ncbi O-GlcNAcylation contributes to augmented vascular reactivity induced by endothelin 1
    Victor V Lima
    Medical College of Georgia, Department of Physiology, 1120 Fifteenth St, CA 3141, Augusta, GA 30912 3000, USA
    Hypertension 55:180-8. 2010
    ..Increased vascular O-GlcNAcylation by ET-1 may represent a mechanism for hypertension-associated vascular dysfunction or other pathological conditions associated with increased levels of ET-1...
  8. ncbi STIM1/Orai1 contributes to sex differences in vascular responses to calcium in spontaneously hypertensive rats
    Fernanda R C Giachini
    Department of Physiology, Georgia Health Sciences University, Augusta, GA, USA
    Clin Sci (Lond) 122:215-26. 2012
    ..Furthermore, female sex hormones may negatively modulate the STIM/Orai1 pathway, contributing to vascular protection observed in female rats...
  9. ncbi Thioredoxin reductase inhibition reduces relaxation by increasing oxidative stress and s-nitrosylation in mouse aorta
    Hyehun Choi
    Department of Physiology, Georgia Health Sciences University, Augusta, GA 30912 3000, USA
    J Cardiovasc Pharmacol 58:522-7. 2011
    ..These data suggest that TrxR regulates vascular relaxation via antioxidant defense and sGC S-nitrosylation. TrxR may be an enzyme to approach for treatment of vascular dysfunction and arterial hypertension...
  10. ncbi Interleukin-10 inhibits the in vivo and in vitro adverse effects of TNF-alpha on the endothelium of murine aorta
    Saiprasad M Zemse
    Department of Physiology, Medical College of Georgia, Augusta, GA 30912, USA
    Am J Physiol Heart Circ Physiol 299:H1160-7. 2010
    ..In conclusion, the anti-inflammatory cytokine IL-10 prevents impairment in endothelium-dependent vasorelaxation caused by TNF-α by protecting eNOS expression...
  11. ncbi Augmented S-nitrosylation contributes to impaired relaxation in angiotensin II hypertensive mouse aorta: role of thioredoxin reductase
    Hyehun Choi
    Department of Physiology, Georgia Health Sciences University, Augusta, Georgia 30912 3000, USA
    J Hypertens 29:2359-68. 2011
    ..We hypothesized that S-nitrosylation is associated with reduced vasodilation in hypertensive mice...
  12. ncbi Upregulation of intermediate calcium-activated potassium channels counterbalance the impaired endothelium-dependent vasodilation in stroke-prone spontaneously hypertensive rats
    Fernanda R C Giachini
    Department of Physiology, Medical College of Georgia, Augusta, GA, USA Department of Pharmacology, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI, USA
    Transl Res 154:183-93. 2009
    ..In conclusion, IK(Ca) channel upregulation, via decreased REST, seems to compensate deficient activity of SK(Ca) channels in the vasculature of spontaneously hypertensive rats...
  13. ncbi Decreased cGMP level contributes to increased contraction in arteries from hypertensive rats: role of phosphodiesterase 1
    Fernanda R Giachini
    Department of Physiology, Medical College of Georgia, Augusta, GA 30912 3000, USA
    Hypertension 57:655-63. 2011
    ..We conclude that PDE1 activation reduces cGMP bioavailability in arteries from Ang II, contributing to increased contractile responsiveness...
  14. ncbi The Rho-A/Rho-kinase pathway is up-regulated but remains inhibited by cyclic guanosine monophosphate-dependent mechanisms during endotoxemia in small mesenteric arteries
    J Eduardo da Silva-Santos
    Department of Physiology, Medical College of Georgia, Augusta, GA, USA
    Crit Care Med 37:1716-23. 2009
    ..We investigated whether a reduced activity in the Rho-A/Rho-kinase pathway could be involved in the impaired vascular reactivity observed in septic shock...
  15. ncbi Impaired vasodilator activity in deoxycorticosterone acetate-salt hypertension is associated with increased protein O-GlcNAcylation
    Victor V Lima
    Medical College of Georgia, Department of Physiology, 1120 Fifteenth St, CA 3141, Augusta, GA 30912 3000, USA
    Hypertension 53:166-74. 2009
    ..Modulation of increased vascular O-GlcNAcylation may represent a novel therapeutic approach in mineralocorticoid hypertension...
  16. ncbi Restoration of endothelin-1-induced impairment in endothelium-dependent relaxation by interleukin-10 in murine aortic rings
    Saiprasad M Zemse
    Department of Physiology, Medical College of Georgia, Augusta, GA 30912 3000, USA
    Can J Physiol Pharmacol 86:557-65. 2008
    ..We conclude that, in murine aorta, the antiinflammatory cytokine IL-10 prevents impairment in endothelium-dependent relaxation induced in response to long-term incubation with ET-1 via normalization of eNOS expression...
  17. ncbi Uridine adenosine tetraphosphate-induced contraction is increased in renal but not pulmonary arteries from DOCA-salt hypertensive rats
    Takayuki Matsumoto
    Dept of Physiology, Georgia Health Sciences Univ, 1120 15th St, Rm CA 3147, Augusta, GA 30912, USA
    Am J Physiol Heart Circ Physiol 301:H409-17. 2011
    ..Up(4)A might be of relevance in the pathophysiology of vascular tone regulation and renal dysfunction in arterial hypertension...
  18. ncbi Increased RhoA/Rho-kinase signaling mediates spontaneous tone in aorta from angiotensin II-induced hypertensive rats
    Liming Jin
    Department of Physiology, Medical College of Georgia, Augusta, GA, USA
    J Pharmacol Exp Ther 318:288-95. 2006
    ..Increased NADPH oxidase-dependent reactive oxygen species may be one of the mechanisms mediating the RhoA/Rho-kinase activation...
  19. ncbi Aorta from angiotensin II hypertensive mice exhibit preserved nitroxyl anion mediated relaxation responses
    Brandi M Wynne
    Department of Physiology, Medical College of Georgia, Augusta, GA 30912, United States
    Pharmacol Res 65:41-7. 2012
    ..These data demonstrate that aorta from AngII-treated hypertensive mice exhibit a preserved relaxation response to AS, an HNO donor, regardless of a significant endothelial dysfunction...
  20. ncbi Mitochondrial aldehyde dehydrogenase prevents ROS-induced vascular contraction in angiotensin-II hypertensive mice
    Hyehun Choi
    Department of Physiology, Georgia Health Sciences University, Augusta, Georgia 30912 3000, USA
    J Am Soc Hypertens 5:154-60. 2011
    ..Because tempol and catalase blocked the contractile response of the ALDH2 inhibitor, ROS generation by AngII may be decreased by ALDH2, thereby preventing ROS-induced contraction...
  21. ncbi Interleukin-10 counteracts impaired endothelium-dependent relaxation induced by ANG II in murine aortic rings
    Saiprasad M Zemse
    Department of Physiology, Medical College of Georgia, Augusta, GA 30912, USA
    Am J Physiol Heart Circ Physiol 292:H3103-8. 2007
    ..We found that the anti-inflammatory cytokine IL-10 prevents impairment in endothelium-dependent vasorelaxation in response to long-term incubation with ANG II via decreasing NADPH oxidase expression...
  22. ncbi Increased PDZ-RhoGEF/RhoA/Rho kinase signaling in small mesenteric arteries of angiotensin II-induced hypertensive rats
    Rob H P Hilgers
    Department of Physiology, Medical College of Georgia, Augusta, Georgia 30912 3000, USA
    J Hypertens 25:1687-97. 2007
    ..Dephosphorylation results in relaxation and is determined by the activity of myosin light chain phosphatase (MLCP), which is negatively regulated by Rho kinase...
  23. ncbi DOCA-salt treatment enhances responses to endothelin-1 in murine corpus cavernosum
    Fernando S Carneiro
    Medical College of Georgia, Department of Physiology, 1120 Fifteenth Street, CA 3141, Augusta, GA 30912 3000, USA
    Can J Physiol Pharmacol 86:320-8. 2008
    ..In summary, DOCA-salt treatment in mice enhances cavernosal reactivity to contractile, but not to relaxant, stimuli, via ET-1/ETA receptor-independent mechanisms...
  24. ncbi Erectile dysfunction in young non-obese type II diabetic Goto-Kakizaki rats is associated with decreased eNOS phosphorylation at Ser1177
    Fernando S Carneiro
    Department of Physiology, Medical College of Georgia, Augusta, GA, USA
    J Sex Med 7:3620-34. 2010
    ..Diabetes mellitus (DM) is a risk factor for erectile dysfunction (ED). Although type 2 DM is responsible for 90-95% diabetes cases, type 1 DM experimental models are commonly used to study diabetes-associated ED...
  25. ncbi O-GlcNAcylation contributes to the vascular effects of ET-1 via activation of the RhoA/Rho-kinase pathway
    Victor V Lima
    Department of Physiology, Medical College of Georgia, 1120 Fifteenth Street, CA 3141, Augusta, GA 30912 3000, USA
    Cardiovasc Res 89:614-22. 2011
    ..Here we tested the hypothesis that O-GlcNAcylation contributes to the vascular effects of ET-1 via activation of the RhoA/Rho-kinase pathway...
  26. ncbi Cytochrome P450 epoxygenases provide a novel mechanism for penile erection
    Liming Jin
    Department of Physiology, Medical College of Georgia, Augusta, Georgia, USA
    FASEB J 20:539-41. 2006
    ..Inhibition of this pathway attenuated erectile function, suggesting that EETs are required for normal erection...
  27. ncbi Nitric oxide induces dilation of rat aorta via inhibition of rho-kinase signaling
    Kanchan Chitaley
    Department of Physiology, Medical College of Georgia, Augusta 30912 3000, USA
    Hypertension 39:438-42. 2002
    ....
  28. ncbi Extracellular signal-regulated kinase (ERK) inhibition decreases arginase activity and improves corpora cavernosal relaxation in streptozotocin (STZ)-induced diabetic mice
    Kenia P Nunes
    Department of Physiology, Georgia Health Sciences University, Augusta, GA 30912, USA
    J Sex Med 8:3335-44. 2011
    ..Both ERK and arginase have been reported to affect the expression and activity of nitric oxide synthase (NOS) and consequently penile erection. Nevertheless, signaling pathways activated by ERK in the penis are not well known...
  29. ncbi Alterations in vasoconstrictor responses to the endothelium-derived contracting factor uridine adenosine tetraphosphate are region specific in DOCA-salt hypertensive rats
    Takayuki Matsumoto
    Department of Physiology, Georgia Health Sciences University, Augusta, GA 30912, USA
    Pharmacol Res 65:81-90. 2012
    ..P2Y receptor activation may contribute to enhancement of Up(4)A-induced contraction in basilar and femoral arteries. These changes in vascular reactivity to Up(4)A may be adaptive to the vascular alterations produced by hypertension...
  30. ncbi Activation of Rho/Rho kinase signaling pathway by reactive oxygen species in rat aorta
    Liming Jin
    Dept of Physiology, Medical College of Georgia, 1120 15th Street, Augusta, GA 30912 3000, USA
    Am J Physiol Heart Circ Physiol 287:H1495-500. 2004
    ..These observations demonstrate that ROS-induced Ca(2+) sensitization is through activation of Rho and a subsequent increase in Rho kinase activity but not Ca(2+)-independent PKC...
  31. ncbi Sympathetic and angiotensin-dependent hypertension during cage-switch stress in mice
    Dexter L Lee
    Department of Physiology, Medical College of Georgia, Augusta, GA 30912-3000, USA
    Am J Physiol Regul Integr Comp Physiol 287:R1394-8. 2004
    ....
  32. ncbi Angiotensin II up-regulates the leukemia-associated Rho guanine nucleotide exchange factor (RhoGEF), a regulator of G protein signaling domain-containing RhoGEF, in vascular smooth muscle cells
    Zhekang Ying
    Department of Physiology, Medical College of Georgia, 1120 Fifteenth St, CA3099, Augusta, GA 30912 3000, USA
    Mol Pharmacol 69:932-40. 2006
    ..These observations support the hypothesis that RhoGEFs, particularly LARG, participate in linking GPCR to RhoA activation in vascular smooth muscle...
  33. ncbi Pregnancy reduces RhoA/Rho kinase and protein kinase C signaling pathways downstream of thromboxane receptor activation in the rat uterine artery
    Styliani Goulopoulou
    Department of Physiology, Georgia Health Sciences University, Augusta, Georgia 30912, USA
    Am J Physiol Heart Circ Physiol 302:H2477-88. 2012
    ..TxA(2)-associated transduction signals and its regulators might present potential targets for the development of new treatments for preeclampsia and other pregnancy-associated vascular diseases...
  34. ncbi PYK2/PDZ-RhoGEF links Ca2+ signaling to RhoA
    Zhekang Ying
    Department of Physiology, Medical College of Georgia, Augusta, GA, USA
    Arterioscler Thromb Vasc Biol 29:1657-63. 2009
    ..Ras homolog gene family member A (RhoA)/Rho-kinase-mediated Ca(2+) sensitization is a critical component of constrictor responses. The present study investigates how angiotensin II activates RhoA...
  35. ncbi Proerectile effects of the Rho-kinase inhibitor (S)-(+)-2-methyl-1-[(4-methyl-5-isoquinolinyl)sulfonyl]homopiperazine (H-1152) in the rat penis
    Cleber E Teixeira
    Department of Physiology, Medical College of Georgia, 1120 15th St, CA 3101, Augusta, GA 30912 3000, USA
    J Pharmacol Exp Ther 315:155-62. 2005
    ..The regulator of G-protein signaling-containing RhoGEFs may represent key components of the molecular mechanisms associated with the abnormal function of the cavernosal smooth muscle...
  36. ncbi Ca2+ sensitization and the regulation of contractility in rat anococcygeus and retractor penis muscle
    Cleber E Teixeira
    Department of Physiology, Medical College of Georgia, Augusta, GA 30912 3000, USA
    Biochem Pharmacol 69:1483-92. 2005
    ..The results indicate that the RhoA/ROK-mediated Ca(2+) sensitization pathway is expressed in anococcygeus and retractor penis muscles and enhances contractions produced by receptor-dependent and independent mechanisms...
  37. ncbi Arginase II deletion increases corpora cavernosa relaxation in diabetic mice
    Haroldo A Toque
    Medical College of Georgia, Department of Pharmacology and Toxicology, Augusta, GA 30912 2300, USA
    J Sex Med 8:722-33. 2011
    ..Because nitric oxide (NO) synthase and Arg share and compete for their substrate L-arginine, NO production is likely linked to regulation of Arg. Arg is highly expressed and implicated in erectile dysfunction...
  38. ncbi Penile erection requires association of soluble guanylyl cyclase with endothelial caveolin-1 in rat corpus cavernosum
    A Elizabeth Linder
    Dept of Physiology, Medical College of Georgia, 1120 Fifteenth St, Augusta, GA 30912 3000, USA
    Am J Physiol Regul Integr Comp Physiol 290:R1302-8. 2006
    ..In summary, our results provide evidence of compartmentalization of sGC in the caveolae of cavernosal endothelial cells contributing to NO signaling mediating smooth muscle relaxation and erection...
  39. ncbi Rho-kinase inhibition reduces pressure-mediated autoregulatory adjustments in afferent arteriolar diameter
    Edward W Inscho
    Department of Physiology, Medical College of Georgia, 1120 15th St, Augusta, Georgia 30912 3000, USA
    Am J Physiol Renal Physiol 296:F590-7. 2009
    ..Thus, Rho-kinase may be important in pressure-mediated autoregulatory adjustments in preglomerular resistance and responsiveness to angiotensin II and autoregulatory P2X1 receptor agonists...
  40. ncbi Disruption of microtubular network attenuates histamine-induced dilation in rat mesenteric vessels
    Carla de Arêdes Brum
    Department of Physiology, Medical College of Georgia, Augusta, Georgia 30912 3000, USA
    Am J Physiol Cell Physiol 288:C443-9. 2005
    ..These results demonstrate the importance of the microtubular system for endothelium-dependent NO-mediated smooth muscle relaxation...
  41. ncbi Phenylalanine improves dilation and blood pressure in GTP cyclohydrolase inhibition-induced hypertensive rats
    Brett M Mitchell
    Department of Physiology, Medical College of Georgia, Augusta, USA
    J Cardiovasc Pharmacol 43:758-63. 2004
    ..These results suggest that L-Phe can reverse GTPCH inhibition in vivo leading to increased vasodilation and decreased blood pressure...
  42. ncbi Glucocorticoids decrease GTP cyclohydrolase and tetrahydrobiopterin-dependent vasorelaxation through glucocorticoid receptors
    Brett M Mitchell
    Department of Physiology, Medical College of Georgia, Augusta, 30912, USA
    J Cardiovasc Pharmacol 43:8-13. 2004
    ..These results support the hypothesis that GTPCH1 downregulation by glucocorticoids is mediated through the GR and contributes to reduced endothelium-dependent relaxation...
  43. ncbi Molecular mechanisms underlying rat mesenteric artery vasorelaxation induced by the nitric oxide-independent soluble guanylyl cyclase stimulators BAY 41-2272 [5-cyclopropyl-2-[1-(2-fluorobenzyl)-1H-pyrazolo[3,4-b]pyridin-3-yl]pyrimidin-4-ylamine] and YC-1
    Cleber E Teixeira
    Department of Physiology, Medical College of Georgia, Augusta, 30912 3000, USA
    J Pharmacol Exp Ther 317:258-66. 2006
    ..The synergistic responses probably reflect the direct effects of NO and NO-independent sGC stimulators on the enzyme, thus representing a potential therapeutic effect by permitting reductions of nitrovasodilator dose...
  44. ncbi Uridine adenosine tetraphosphate induces contraction and relaxation in rat aorta
    A Elizabeth Linder
    Department of Physiology, Medical College of Georgia, Augusta, GA 30912 3000, USA
    Vascul Pharmacol 48:202-7. 2008
    ..Up(4)A-induced contraction is modulated by nitric oxide production, mediated by P1 and P2X receptor activation, and involves L-type Ca(2+) channels, Rho-kinase pathway and superoxide formation...
  45. ncbi Internal pudendal artery from type 2 diabetic female rats demonstrate elevated endothelin-1-mediated constriction
    Kyan J Allahdadi
    Department of Physiology, Georgia Health Sciences University, Augusta, GA 30912, USA
    J Sex Med 8:2472-83. 2011
    ..The internal pudendal artery supplies blood to the vagina and clitoris and diabetes-associated functional abnormalities in this vascular bed may contribute to FSD...
  46. ncbi Increaseed expression of mRNA for regulator of G protein signaling domain-containing Rho guanine nucleotide exchange factors in aorta from stroke-prone spontaneously hypertensive rats
    Zhekang Ying
    Department of Physiology, Medical College of Georgia, Augusta, Georgia, USA
    Am J Hypertens 17:981-5. 2004
    ..The RhoGEF, providing this coupling, contain the regulator of G protein signaling (RGS) domain. We hypothesized higher mRNA expression levels for these RhoGEF in aorta from stroke-prone spontaneously hypertensive rats (SHRSP)...
  47. ncbi Hypertension and RhoA/Rho-kinase signaling in the vasculature: highlights from the recent literature
    Dexter L Lee
    Department of Physiology, Medical College of Georgia, Augusta 30912-3000, USA
    Hypertension 44:796-9. 2004
    ..This brief review will highlight recent studies demonstrating a role for the RhoA/Rho-kinase signaling pathway in the increased vasoconstriction characteristic of hypertension...
  48. ncbi Dynamic association of nitric oxide downstream signaling molecules with endothelial caveolin-1 in rat aorta
    A Elizabeth Linder
    Medical College of Georgia, Department of Physiology CA 3101, 1120 Fifteenth St, Augusta, GA 30912 3000, USA
    J Pharmacol Exp Ther 314:9-15. 2005
    ..In summary, our results provide evidence of compartmentalization of sGC, PKG, and PKA in endothelial caveolae contributing to NO signaling cascade, giving new insights by which the endothelium mediates vascular smooth muscle relaxation...
  49. ncbi Pyk2 mediates increased adrenergic contractile responses in arteries from DOCA-salt mice - VASOACTIVE PEPTIDE SYMPOSIUM
    Fernanda R C Giachini
    Medical College of Georgia, Department of Physiology, Augusta, GA, USA, 30904
    J Am Soc Hypertens 2:431-8. 2008
    ..We tested the hypothesis that enhanced vascular reactivity in DOCA-salt hypertensive mice are due to increased activation of Pyk2...
  50. ncbi Sepiapterin decreases vasorelaxation in nitric oxide synthase inhibition-induced hypertension
    Brett M Mitchell
    Department of Physiology, Medical College of Georgia, Augusta, Georgia 30912, USA
    J Cardiovasc Pharmacol 43:93-8. 2004
    ..These results support the hypothesis that sepiapterin further reduces vasodilation in the presence of NOS inhibition and may be caused by BH4 autoxidation...
  51. ncbi Neurophysiological basis of penile erection
    Fernanda B M Priviero
    Department of Physiology, Medical College of Georgia, 1120 15th Street, CA 3101 Augusta, GA 30912 3000, USA
    Acta Pharmacol Sin 28:751-5. 2007
    ....
  52. ncbi Increased vascular O-GlcNAcylation augments reactivity to constrictor stimuli - VASOACTIVE PEPTIDE SYMPOSIUM
    Victor V Lima
    Department of Physiology, Medical College of Georgia, Augusta, GA, 30912 3000, USA
    J Am Soc Hypertens 2:410-7. 2008
    ..Because proteins that play a role in vascular tone regulation can be O-GlcNAcylated, we hypothesized that O-GlcNAcylation increases vascular reactivity to constrictor stimuli...
  53. ncbi Reduced expression of SKCa and IKCa channel proteins in rat small mesenteric arteries during angiotensin II-induced hypertension
    Rob H P Hilgers
    Department of Physiology, Medical College of Georgia, Augusta, GA, USA
    Am J Physiol Heart Circ Physiol 292:H2275-84. 2007
    ..The role of IK1 channels is less clear but might compensate for reduced SK3 activity...
  54. ncbi S-nitrosylation Inhibits protein kinase C-mediated contraction in mouse aorta
    Hyehun Choi
    Department of Physiology, Medical College of Georgia, Augusta, GA 30912 3000, USA
    J Cardiovasc Pharmacol 57:65-71. 2011
    ..Because S-nitrosylation of PKC inhibits phosphorylation and activation of target proteins related to contraction, this posttranslational modification may be a key player in conditions of decreased vascular reactivity...
  55. ncbi GTP cyclohydrolase 1 inhibition attenuates vasodilation and increases blood pressure in rats
    Brett M Mitchell
    Department of Physiology CL 3162, Medical College of Georgia, 1120 15th Street, Augusta, GA 30912, USA
    Am J Physiol Heart Circ Physiol 285:H2165-70. 2003
    ..These results support the hypothesis that GTP cyclohydrolase 1 inhibition decreases relaxation and increases blood pressure in rats...
  56. ncbi Erectile function is improved in aged rats by PnTx2-6, a toxin from Phoneutria nigriventer spider venom
    Kenia P Nunes
    Department of Physiology, Georgia Health Sciences University, Augusta, GA, USA
    J Sex Med 9:2574-81. 2012
    ..This spider's venom is characterized by several symptoms, including erection. PnTx2-6 has been implicated in this phenomenon. Animal venoms have been postulated as potential drugs to treat ED...
  57. ncbi Angiotensin II utilizes Janus kinase 2 in hypertension, but not in the physiological control of blood pressure, during low-salt intake
    Amy K L Banes-Berceli
    Department of Physiology, Medical College of Georgia, Augusta, Georgia, USA
    Am J Physiol Regul Integr Comp Physiol 301:R1169-76. 2011
    ....
  58. ncbi P2X7 receptor activation amplifies lipopolysaccharide-induced vascular hyporeactivity via interleukin-1 beta release
    Chin Wei Chiao
    Department of Physiology, Medical College of Georgia, 1120 15th Street, Augusta, GA 30912, USA
    J Pharmacol Exp Ther 326:864-70. 2008
    ..Thus, activation of P2X(7) receptor amplifies LPS-induced hyporeactivity in mouse endothelium-intact aorta, which is associated with IL-1beta-mediated release of nitric oxide by iNOS...
  59. ncbi O-GlcNAcylation: a novel pathway contributing to the effects of endothelin in the vasculature
    Victor V Lima
    Department of Physiology, Medical College of Georgia, Augusta, Georgia, USA
    Am J Physiol Regul Integr Comp Physiol 300:R236-50. 2011
    ..ET-1-induced O-GlcNAcylation and its implications for vascular function are then discussed. Finally, the interplay between O-GlcNAcylation and O-phosphorylation is addressed...
  60. ncbi STIM and Orai proteins: players in sexual differences in hypertension-associated vascular dysfunction?
    Fernanda R C Giachini
    Department of Physiology, Medical College of Georgia, Augusta, GA 30912 3000, U S A
    Clin Sci (Lond) 118:391-6. 2010
    ....
  61. ncbi GTP cyclohydrolase 1 downregulation contributes to glucocorticoid hypertension in rats
    Brett M Mitchell
    Department of Physiology, CL 3162, Medical College of Georgia, Augusta, GA 30912, USA
    Hypertension 41:669-74. 2003
    ..These results support the hypothesis that downregulation of GTP cyclohydrolase 1 contributes to increased blood pressure in glucocorticoid hypertensive rats...
  62. ncbi Increased vascular sensitivity and connexin43 expression after sympathetic denervation
    David P Slovut
    Department of Physiology, University of Michigan, Ann Arbor, MI, USA
    Cardiovasc Res 62:388-96. 2004
    ..05). Conclusion: We conclude that sympathetic denervation results in increased expression of Cx43, which in turn, contributes to increased spontaneous and agonist-induced OA in VSM...
  63. ncbi Endothelin-1 induces contraction of female rat internal pudendal and clitoral arteries through ET(A) receptor and rho-kinase activation
    Kyan J Allahdadi
    Physiology, Medical College of Georgia Physiology, Augusta, GA 30912, USA
    J Sex Med 7:2096-103. 2010
    ..The internal pudendal artery (IPA) supplies blood to the vagina and clitoris. Inadequate blood flow through the IPA may lead to insufficient vaginal engorgement and clitoral tumescence...
  64. ncbi Hypertensive response to acute stress is attenuated in interleukin-6 knockout mice
    Dexter L Lee
    Department of Physiology, Medical College of Georgia, Augusta, GA 30912 3000, USA
    Hypertension 44:259-63. 2004
    ....
  65. ncbi Decreased epoxygenase and increased epoxide hydrolase expression in the mesenteric artery of obese Zucker rats
    Xueying Zhao
    Vascular Biology Center, Medical College of Georgia, Augusta, GA 30912-2500, USA
    Am J Physiol Regul Integr Comp Physiol 288:R188-96. 2005
    ....
  66. ncbi Regional heterogeneity in acetylcholine-induced relaxation in rat vascular bed: role of calcium-activated K+ channels
    Rob H P Hilgers
    Department of Physiology, Medical College of Georgia, Augusta, GA 30912, USA
    Am J Physiol Heart Circ Physiol 291:H216-22. 2006
    ..Our data demonstrate regional heterogeneity in SKCa and IKCa function and gene expression and stress the importance of these channels in smaller resistance-sized arteries, where the role of EDHF is more pronounced...
  67. ncbi Increased nitric oxide synthase activity and Hsp90 association in skeletal muscle following chronic exercise
    M Brennan Harris
    Vascular Biology Center, Medical College of Georgia, Augusta, GA 30912 2500, USA
    Eur J Appl Physiol 104:795-802. 2008
    ..This interaction may play a role in altering muscle blood flow and skeletal muscle redox status...
  68. ncbi Differential effects of the phosphodiesterase type 5 inhibitors sildenafil, vardenafil, and tadalafil in rat aorta
    Cleber E Teixeira
    Department of Physiology, Medical College of Georgia, Augusta, 30912 3000, USA
    J Pharmacol Exp Ther 316:654-61. 2006
    ..These results suggest that vardenafil, but not sildenafil or tadalafil, affects Ca(2+) handling in the rat aorta in addition to increasing cGMP levels through inhibition of PDE5 to cause relaxation...
  69. ncbi Microtubule depolymerization facilitates contraction of rat aorta via activation of Rho-kinase
    Kanchan Chitaley
    Department of Physiology, University of Michigan, Ann Arbor, MI 48019, USA
    Vascul Pharmacol 38:157-61. 2002
    ..These results support the hypothesis that MT depolymerization facilitates contraction of rat aorta via activation of Rho-kinase...
  70. ncbi Therapeutic targets in hypertension: is there a place for antagonists of the most potent vasoconstrictors?
    Fernanda R C Giachini
    Medical College of Georgia, Department of Physiology, 1120 Fifteenth Street, CA 3141 Augusta, Georgia 30912 3000, USA
    Expert Opin Ther Targets 12:327-39. 2008
    ..Endothelin-1 (ET-1) and urotensin II (U-II) are the most potent and unusually long-lasting constrictors of human vessels known to date...
  71. ncbi RhoA/Rho-kinase and vascular diseases: what is the link?
    Kenia Pedrosa Nunes
    Department of Physiology, Medical College of Georgia, Augusta, GA 30912, USA
    Cell Mol Life Sci 67:3823-36. 2010
    ..We will briefly describe the ROCK isoforms and the role of RhoA/Rho-kinase in the vasculature, before exploring the most recent findings regarding this pathway and various diseases...
  72. ncbi Determination of adenosine effects and adenosine receptors in murine corpus cavernosum
    Rita C Tostes
    Department of Pharmacology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil
    J Pharmacol Exp Ther 322:678-85. 2007
    ..In addition, adenosine negatively modulates sympathetic neurotransmission, by A1 receptor subtype activation, in murine corpora cavernosa. Adenosine may subserve dual roles in modulating the physiological mechanisms of erection in mice...
  73. ncbi Inhibition of tonic contraction--a novel way to approach erectile dysfunction
    Thomas M Mills
    Department of Physiology, Medical College of Georgia, Augusta, 30912-3000, USA
    J Androl 23:S5-9. 2002
  74. ncbi Altered vascular reactivity in mice made hypertensive by nitric oxide synthase inhibition
    A Elizabeth Linder
    Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI, USA
    J Cardiovasc Pharmacol 46:438-44. 2005
    ..We conclude that mice treated with L-NNA develop hypertension and that a reduction in NO availability is responsible for the changes observed in vascular reactivity...
  75. ncbi Nitric oxide inhibits RhoA/Rho-kinase signaling to cause penile erection
    Thomas M Mills
    Department of Physiology, Medical College of Georgia, Augusta, GA, USA
    Eur J Pharmacol 439:173-4. 2002
    ..These results support the hypothesis that NO inhibits Rho-kinase-induced cavernosal vasoconstriction during erection...
  76. ncbi Inhibition of the tonic contraction in the treatment of erectile dysfunction
    Liming Jin
    Department of Physiology, Medical College of Georgia, Augusta, Georgia 30912 3000, USA
    Expert Opin Ther Targets 7:265-76. 2003
    ..Studies have shown that inhibition of the RhoA/Rho-kinase signalling pathway induces penile erection. Further understanding of this RhoA/Rho-kinase pathway may provide a novel alternative treatment for ED...
  77. ncbi Cigarette smoking and erectile dysfunction: focus on NO bioavailability and ROS generation
    Rita C Tostes
    University of Sao Paulo, Department of Pharmacology, Sao Paulo, SP, Brazil
    J Sex Med 5:1284-95. 2008
    ..Considered a major public health problem, which seriously affects the quality of life of patients and their partners, ED becomes increasingly prevalent with age and chronic smoking is a major risk factor in the development of ED...
  78. ncbi Adenosine actions are preserved in corpus cavernosum from obese and type II diabetic db/db mouse
    Fernando Silva Carneiro
    Pharmacology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil
    J Sex Med 5:1156-66. 2008
    ..Whereas the nonadrenergic-noncholinergic (NANC)/neurogenic nitric oxide pathway has received great attention in diabetes-associated ED, few studies have addressed sympathetic overactivity...
  79. ncbi TNF-alpha knockout mice have increased corpora cavernosa relaxation
    Fernando S Carneiro
    Pharmacology, Institute of Biomedical Sciences, University of Sao Paulo Sao Paulo, SP, Brazil
    J Sex Med 6:115-25. 2009
    ..Tumor necrosis factor-alpha (TNF-alpha), a pro-inflammatory cytokine, suppresses endothelial nitric oxide synthase (eNOS) expression...
  80. ncbi Vasoactive factors in sickle cell disease: in vitro evidence for endothelin-1-mediated vasoconstriction
    Sitki Ergul
    Division of Hematology and Oncology, Medical University of South Carolina, Charleston, SC, USA
    Am J Hematol 76:245-51. 2004
    ....
  81. ncbi Female sexual dysfunction: therapeutic options and experimental challenges
    Kyan J Allahdadi
    Department of Physiology, Medical College of Georgia, Augusta, Georgia 30912, USA
    Cardiovasc Hematol Agents Med Chem 7:260-9. 2009
    ..The aim of this review is to provide the most current knowledge on FSD, advances in basic science addressing this dysfunction, and explore developing therapeutic options...
  82. ncbi TNF-alpha infusion impairs corpora cavernosa reactivity
    Fernando S Carneiro
    Institute of Biomedical Sciences, University of Sao Paulo, Pharmacology, Sao Paulo, SP, Brazil
    J Sex Med 6:311-9. 2009
    ..Erectile dysfunction (ED), as well as cardiovascular diseases (CVDs), is associated with endothelial dysfunction and increased levels of proinflammatory cytokines, such as tumor necrosis factor-alpha (TNF-alpha)...
  83. ncbi Smooth muscle contraction and relaxation
    R Clinton Webb
    Department of Physiology, Medical College of Georgia, Augusta, Georgia 30912, USA
    Adv Physiol Educ 27:201-6. 2003
    ..Removal of Ca(2+) from the cytosol and stimulation of myosin phosphatase initiate the process of smooth muscle relaxation...
  84. ncbi [Inhibition of tonic contraction of smooth muscle: a new approach to achieve erection dysfunction]
    Yu-Tian Dai
    Department of Urology, Affiliated Drum Tower Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210008, China
    Zhonghua Nan Ke Xue 8:391-4. 2002
    ..In addition, our results show that topical application of Y-27632 may be an effective mode of treatment for erectile dysfunction...
  85. ncbi Abnormal activation of Na+-K+ pump in aortas from rats with endotoxaemia
    Shiu-Jen Chen
    Department of Physiology, National Defence Medical Centre, Taipei, Taiwan, ROC
    Naunyn Schmiedebergs Arch Pharmacol 368:57-62. 2003
    ..These results suggest that the Na(+)-K(+) pump in the vascular bed of animals with endotoxic shock is abnormally activated and this augmented activation is modulated by cGMP...
  86. ncbi Impaired vasodilation and nitric oxide synthase activity in glucocorticoid-induced hypertension
    Brett M Mitchell
    Department of Physiology at the Medical College of Georgia, Augusta 30912-3000, USA
    Biol Res Nurs 4:16-21. 2002
    ..In the future, tetrahydrobiopterin supplementation in patients with iatrogenic Cushing's syndrome may prove to be beneficial and decrease mortality attributed to cardiovascular disease...
  87. ncbi Stimulation of soluble guanylyl cyclase by BAY 41-2272 relaxes anococcygeus muscle: interaction with nitric oxide
    Cleber E Teixeira
    Department of Pharmacology, Faculty of Medical Sciences, UNICAMP, Campinas (SP, Brazil
    Eur J Pharmacol 530:157-65. 2006
    ..The relaxations induced by glyceryl trinitrate were also significantly potentiated by BAY 41-2272. In conclusion, BAY 41-2272 interacts with endogenous and exogenous NO causing a potent relaxation of rat anococcygeus muscle...
  88. ncbi Adeno-associated viral gene transfer of dominant negative RhoA enhances erectile function in rats
    Kanchan Chitaley
    Department of Physiology, Medical College of Georgia, Augusta, GA 30912, USA
    Biochem Biophys Res Commun 298:427-32. 2002
    ..The novel findings of this study demonstrate a functional role of RhoA in maintaining the flaccid penis and provide support for the inhibition of RhoA as a potential therapy for the enhancement of erectile function...
  89. ncbi Neuroprotection by tempol in a model of iron-induced oxidative stress in acute ischemic stroke
    Shyamal H Mehta
    Dept. of Physiology, Medical College of Georgia, 1120 15th St, CL2122, Augusta, GA 30912, USA
    Am J Physiol Regul Integr Comp Physiol 286:R283-8. 2004
    ..We propose that iron-induced ROS generation in the cerebral vasculature adds to oxidative stress during an ischemic episode after the disruption of the blood-brain barrier...
  90. ncbi Effects of 5-cyclopropyl-2-[1-(2-fluoro-benzyl)-1H-pyrazolo[3,4-b]pyridine-3-yl]pyrimidin-4-ylamine (BAY 41-2272) on smooth muscle tone, soluble guanylyl cyclase activity, and NADPH oxidase activity/expression in corpus cavernosum from wild-type, neuronal
    Cleber E Teixeira
    Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, Campinas, Sao Paulo, Brazil
    J Pharmacol Exp Ther 322:1093-102. 2007
    ....
  91. ncbi Comparative pharmacological analysis of Rho-kinase inhibitors and identification of molecular components of Ca2+ sensitization in the rat lower urinary tract
    Cleber E Teixeira
    Department of Pharmacology, Faculty of Medical Sciences, UNICAMP, Campinas, SP, Brazil
    Biochem Pharmacol 74:647-58. 2007
    ..Manipulation of RhoGEF expression may provide further understanding of mechanisms involving Ca(2+) sensitization in the lower urinary tract...
  92. ncbi Methyl-beta-cyclodextrin prevents angiotensin II-induced tachyphylactic contractile responses in rat aorta
    A Elizabeth Linder
    Department of Pharmacology and Toxicology, Michigan State University, B 445 Life Sciences Building, East Lansing, MI 48824 1317, USA
    J Pharmacol Exp Ther 323:78-84. 2007
    ..Our data suggest that caveolae are involved in the tachyphylactic contractile response induced by angiotensin II in rat aorta, and this effect is related to receptor internalization...
  93. ncbi Endothelin, sex and hypertension
    Rita C Tostes
    Department of Pharmacology, Institute of Biomedical Sciences, University of Sao Paulo, 05508 900 Sao Paulo, Brazil
    Clin Sci (Lond) 114:85-97. 2008
    ..With further insights into these differences, we may learn that men and women could require different antihypertensive regimens...
  94. ncbi NADPH oxidase activation: a mechanism of hypertension-associated erectile dysfunction
    Liming Jin
    Urology Department, Johns Hopkins University, Baltimore, MD, USA
    J Sex Med 5:544-51. 2008
    ..Hypertension is a risk factor for erectile dysfunction (ED). The pathophysiologic basis of ED in hypertension remains largely unknown...
  95. ncbi Comparison of the involvement of protein kinase C in agonist-induced contractions in mouse aorta and corpus cavernosum
    Liming Jin
    Department of Internal Medicine, Division of Endocrinology, Clinical Nutrition and Vascular Medicine, University of California, Davis, CA 95616, United States
    Eur J Pharmacol 590:363-8. 2008
    ..Taken together, our data indicate that PKC does not have a significant role in agonist-induced contractions in mouse corpus cavernosum, whereas it mediates the contractile response to agonists in the aorta...
  96. ncbi [Effect of topical application of a Rho-kinase inhibitor on the erectile response in rats]
    Yutian Dai
    Department of Urology, the Affiliated Drum Tower Hospital of Nanjing University Medical College, Nanjing, Jiangsu 210008, China
    Zhonghua Nan Ke Xue 10:589-92. 2004
    ..To investigate the effect of Rho-kinase inhibitor applied topically on the penile erection and systemic circulation of rats...
  97. ncbi Evidence that the vasodilator angiotensin-(1-7)-Mas axis plays an important role in erectile function
    Andrey C da Costa Goncalves
    Department of Physiology, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
    Am J Physiol Heart Circ Physiol 293:H2588-96. 2007
    ..Furthermore, the attenuated erectile function of DOCA-salt hypertensive rats was fully restored by ANG-(1-7) administration. Together these data provide strong evidence for a key role of the ANG-(1-7)-Mas axis in erectile function...
  98. ncbi RhoA/Rho-kinase suppresses endothelial nitric oxide synthase in the penis: a mechanism for diabetes-associated erectile dysfunction
    Trinity J Bivalacqua
    Department of Urology, Tulane Health Sciences Center, New Orleans, LA 70112, USA
    Proc Natl Acad Sci U S A 101:9121-6. 2004
    ..Importantly, these data imply that inhibition of RhoA/Rho-kinase improves eNOS protein content and activity thus restoring erectile function in diabetes...
  99. ncbi Cold-induced vasoconstriction: a Waltz pairing Rho-kinase signaling and alpha2-adrenergic receptor translocation
    Cleber E Teixeira
    Circ Res 94:1273-5. 2004
  100. ncbi Vasorelaxing effects of propranolol in rat aorta and mesenteric artery: a role for nitric oxide and calcium entry blockade
    Fernanda B M Priviero
    Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, Campinas, SP, Brazil
    Clin Exp Pharmacol Physiol 33:448-55. 2006
    ..8. In conclusion, DL-propranolol relaxes arterial smooth muscle by mechanisms involving activation of the NO-cGMP pathway and calcium influx blockade, independent of beta-adrenoceptor blockade...