Gabriel Virella

Summary

Affiliation: Medical University of South Carolina
Country: USA

Publications

  1. pmc The Pathogenic Role of the Adaptive Immune Response to Modified LDL in Diabetes
    Gabriel Virella
    Department of Microbiology and Immunology, Medical University of South Carolina Charleston, SC, USA
    Front Endocrinol (Lausanne) 3:76. 2012
  2. doi request reprint Immunoassay of modified forms of human low density lipoprotein in isolated circulating immune complexes
    Gabriel Virella
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC 29425, USA
    J Immunoassay Immunochem 34:61-74. 2013
  3. pmc Development of capture assays for different modifications of human low-density lipoprotein
    Gabriel Virella
    Medical University of South Carolina, Department of Microbiology and Immunology, 173 Ashley Ave, PO Box 250504, Charleston, SC 29425, USA
    Clin Diagn Lab Immunol 12:68-75. 2005
  4. ncbi request reprint Definition of the immunogenic forms of modified human LDL recognized by human autoantibodies and by rabbit hyperimmune antibodies
    Gabriel Virella
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC, USA
    J Lipid Res 45:1859-67. 2004
  5. pmc Atherogenesis and the humoral immune response to modified lipoproteins
    Gabriel Virella
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC 29425, United States
    Atherosclerosis 200:239-46. 2008
  6. pmc Distribution of IgM and IgG antibodies to oxidized LDL in immune complexes isolated from patients with type 1 diabetes and its relationship with nephropathy
    Gabriel Virella
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC 29425, USA
    Clin Immunol 127:394-400. 2008
  7. pmc Lipoprotein autoantibodies: measurement and significance
    Gabriel Virella
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, 29425, USA
    Clin Diagn Lab Immunol 10:499-505. 2003
  8. ncbi request reprint Autoimmune response to advanced glycosylation end-products of human LDL
    Gabriel Virella
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC 92425, USA
    J Lipid Res 44:487-93. 2003
  9. pmc Heat shock protein 70B' (HSP70B') expression and release in response to human oxidized low density lipoprotein immune complexes in macrophages
    Kent J Smith
    Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 285:15985-93. 2010
  10. pmc LDL-containing immune complexes in the DCCT/EDIC cohort: associations with lipoprotein subclasses
    Richard L Klein
    Division of Endocrinology, Metabolism, and Medical Genetics, Department of Medicine, Medical University of South Carolina, Charleston, SC 29403, USA
    J Diabetes Complications 25:73-82. 2011

Collaborators

Detail Information

Publications30

  1. pmc The Pathogenic Role of the Adaptive Immune Response to Modified LDL in Diabetes
    Gabriel Virella
    Department of Microbiology and Immunology, Medical University of South Carolina Charleston, SC, USA
    Front Endocrinol (Lausanne) 3:76. 2012
    ..But mLDL-IC are likely to reach higher concentrations and have a more prominent pathogenic role in diabetes due to increased antigenic load secondary to high oxidative stress and to enhanced autoimmune responses in type 1 diabetes...
  2. doi request reprint Immunoassay of modified forms of human low density lipoprotein in isolated circulating immune complexes
    Gabriel Virella
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC 29425, USA
    J Immunoassay Immunochem 34:61-74. 2013
    ....
  3. pmc Development of capture assays for different modifications of human low-density lipoprotein
    Gabriel Virella
    Medical University of South Carolina, Department of Microbiology and Immunology, 173 Ashley Ave, PO Box 250504, Charleston, SC 29425, USA
    Clin Diagn Lab Immunol 12:68-75. 2005
    ..This approach obviates the interference of IC in previously reported modified LDL assays and allows determination of the degree of modification of LDL with greater accuracy...
  4. ncbi request reprint Definition of the immunogenic forms of modified human LDL recognized by human autoantibodies and by rabbit hyperimmune antibodies
    Gabriel Virella
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC, USA
    J Lipid Res 45:1859-67. 2004
    ....
  5. pmc Atherogenesis and the humoral immune response to modified lipoproteins
    Gabriel Virella
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC 29425, United States
    Atherosclerosis 200:239-46. 2008
    ..There is no conclusive evidence supporting a protective role for IgM antibodies in humans, possibly because autoantibodies to modified lipoproteins are predominantly of the IgG isotype...
  6. pmc Distribution of IgM and IgG antibodies to oxidized LDL in immune complexes isolated from patients with type 1 diabetes and its relationship with nephropathy
    Gabriel Virella
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC 29425, USA
    Clin Immunol 127:394-400. 2008
    ..In conclusion, the predominance of pro-inflammatory IgG oxLDL antibodies is associated with existence of diabetic nephropathy, and a protective role of IgM antibodies could not be demonstrated...
  7. pmc Lipoprotein autoantibodies: measurement and significance
    Gabriel Virella
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, 29425, USA
    Clin Diagn Lab Immunol 10:499-505. 2003
  8. ncbi request reprint Autoimmune response to advanced glycosylation end-products of human LDL
    Gabriel Virella
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC 92425, USA
    J Lipid Res 44:487-93. 2003
    ..A comparative study of the reactivity of purified AGE-LDL antibodies with CML-LDL and CML-serum albumin showed no cross-reactivity...
  9. pmc Heat shock protein 70B' (HSP70B') expression and release in response to human oxidized low density lipoprotein immune complexes in macrophages
    Kent J Smith
    Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 285:15985-93. 2010
    ..The data also implicate HSP70B' in key cellular functions, such as regulation of SK1 activity and release of IL-10, which influence macrophage activation and survival...
  10. pmc LDL-containing immune complexes in the DCCT/EDIC cohort: associations with lipoprotein subclasses
    Richard L Klein
    Division of Endocrinology, Metabolism, and Medical Genetics, Department of Medicine, Medical University of South Carolina, Charleston, SC 29403, USA
    J Diabetes Complications 25:73-82. 2011
    ....
  11. pmc Risk factors related to inflammation and endothelial dysfunction in the DCCT/EDIC cohort and their relationship with nephropathy and macrovascular complications
    Maria F Lopes-Virella
    Department of Medicine and Laboratory Services, Medical University of South Carolina and Ralph H Johnson VA Medical Center, Charleston, South Carolina, USA
    Diabetes Care 31:2006-12. 2008
    ....
  12. ncbi request reprint The immunogenicity of modified lipoproteins
    Maria F Lopes-Virella
    Strom Thurmond Research Building, 114 Doughty Street, Room 529, Charleston, SC 29425, USA
    Ann N Y Acad Sci 1043:367-78. 2005
    ..In conclusion, isolation and characterization of LDL-ICs appears to yield information of significant value that is not derived from other approaches to measure LDL modifications and their corresponding antibodies in humans...
  13. pmc High concentrations of AGE-LDL and oxidized LDL in circulating immune complexes are associated with progression of retinopathy in type 1 diabetes
    Maria F Lopes-Virella
    Department of Medicine, Medical University of South Carolina, Charleston, South Carolina, USA
    Diabetes Care 35:1333-40. 2012
    ..To determine whether immunocomplexes (ICs) containing advanced glycation end product (AGE)-LDL (AGE-LDL) and oxidized LDL (oxLDL) contribute to the development of retinopathy over a 16-year period in subjects with type 1 diabetes...
  14. pmc High levels of oxidized LDL in circulating immune complexes are associated with increased odds of developing abnormal albuminuria in Type 1 diabetes
    Maria F Lopes-Virella
    Department of Medicine and Laboratory Services, Medical University of South Carolina and Ralph H Johnson VA Medical Center, Charleston, SC, USA
    Nephrol Dial Transplant 27:1416-23. 2012
    ..Modified low-density lipoprotein (LDL) immune complexes (IC) have proinflammatory properties and play a role in albuminuria development...
  15. pmc The levels of MDA-LDL in circulating immune complexes predict myocardial infarction in the VADT study
    Maria F Lopes-Virella
    Department of Medicine, Medical University of South Carolina, Charleston, SC, United States
    Atherosclerosis 224:526-31. 2012
    ..The present study was undertaken to determine whether the levels of oxidized LDL (oxLDL), malondialdehyde-LDL (MDA-LDL) and advanced glycation end products-LDL (AGE-LDL) in IC predict incident CVD events in type 2 diabetes (VADT cohort)...
  16. ncbi request reprint Immune complexes containing modified lipoproteins are related to the progression of internal carotid intima-media thickness in patients with type 1 diabetes
    Maria F Lopes-Virella
    Ralph H Johnson Department of Veterans Affairs Medical Center, Charleston, SC, USA
    Atherosclerosis 190:359-69. 2007
    ..In conclusion these data demonstrate that increased levels of modified ApoB-rich IC are associated with increased progression of internal carotid IMT in the DCCT/EDIC cohort of type 1 diabetes...
  17. ncbi request reprint OxLDL immune complexes activate complement and induce cytokine production by MonoMac 6 cells and human macrophages
    Antonio F Saad
    Ralph H Johnson Department of Veterans Affairs Medical Center, Charleston, SC, USA
    J Lipid Res 47:1975-83. 2006
    ..In conclusion, human OxLDL-ICs have proinflammatory properties, as reflected by their capacity to activate the classical pathway of complement and to induce proinflammatory cytokine release from MM6 cells and primary human macrophages...
  18. pmc Clinical significance of the humoral immune response to modified LDL
    Maria F Lopes-Virella
    Department of Medicine, Medical University of South Carolina, Charleston, SC, USA
    Clin Immunol 134:55-65. 2010
    ..The possibility that spontaneously formed or induced modified LDL antibodies (particularly IgM oxLDL antibodies) may have a protective effect has been suggested, but the data is unclear and needs to be further investigated...
  19. pmc Oxidized LDL and AGE-LDL in circulating immune complexes strongly predict progression of carotid artery IMT in type 1 diabetes
    Kelly J Hunt
    Department of Public Health Sciences, Medical University of South Carolina, Charleston, SC, United States Ralph H Johnson VA Medical Center, Charleston, SC, United States Electronic address
    Atherosclerosis 231:315-22. 2013
    ..Over 90% of modified LDL in circulation is associated to specific antibodies circulating as part of immune complexes (IC); however, few studies have examined their relationship with cardiovascular disease...
  20. pmc Baseline markers of inflammation are associated with progression to macroalbuminuria in type 1 diabetic subjects
    Maria F Lopes-Virella
    Department of Medicine and Laboratory Services, Medical University of South Carolina and Ralph H Johnson VA Medical Center, Charleston, South Carolina, USA
    Diabetes Care 36:2317-23. 2013
    ....
  21. pmc Oxidized LDL immune complexes and oxidized LDL differentially affect the expression of genes involved with inflammation and survival in human U937 monocytic cells
    Samar M Hammad
    Department of Cell Biology and Anatomy, Medical University of South Carolina, 114 Doughty Street, 630B, P O Box 250776, Charleston, SC 29425, USA
    Atherosclerosis 202:394-404. 2009
    ..To compare the global effects of oxidized LDL (oxLDL) and oxLDL-containing immune complexes (oxLDL-IC) on gene expression in human monocytic cells and to identify differentially expressed genes involved with inflammation and survival...
  22. pmc Oxidized LDL immune complexes stimulate collagen IV production in mesangial cells via Fc gamma receptors I and III
    Souzan A Abdelsamie
    Division of Endocrinology, Diabetes and Medical Genetics, Department of Medicine, College of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA
    Clin Immunol 139:258-66. 2011
    ..Taken together, this study showed that oxLDL-IC stimulated collagen IV in mesangial cells via FcγRI and FcγRIII, and the expression of FcγRI was increased by oxLDL-IC...
  23. pmc Differential regulation of acid sphingomyelinase in macrophages stimulated with oxidized low-density lipoprotein (LDL) and oxidized LDL immune complexes: role in phagocytosis and cytokine release
    Jean Philip Truman
    Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC 29425, USA
    Immunology 136:30-45. 2012
    ..These findings may contribute to increased understanding of mechanisms mediating macrophage involvement in atherosclerosis...
  24. ncbi request reprint Role of simvastatin as an immunomodulator in type 2 diabetes
    Maria F Lopes-Virella
    Department of Medicine, Medical University of South Carolina, and Ralph H Johnson Department of Veterans Affairs Medical Center, Charleston, South Carolina 29425, USA
    Diabetes Care 27:908-13. 2004
    ....
  25. ncbi request reprint The role of immune and inflammatory processes in the development of macrovascular disease in diabetes
    Maria F Lopes-Virella
    Ralph H Jonhson VA Medical Center and Department of Medicine, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Front Biosci 8:s750-68. 2003
    ..These autoantibodies to modified lipoproteins have sufficient affinity to form stable antigen-antibody complexes, which have been also shown to have pro-atheromatous and pro-inflammatory properties...
  26. ncbi request reprint LDL immune complexes stimulate LDL receptor expression in U937 histiocytes via extracellular signal-regulated kinase and AP-1
    Yuchang Fu
    Ralph H Johnson Veterans Administration Medical Center, Charleston, SC 29401, USA
    J Lipid Res 44:1315-21. 2003
    ..In conclusion, the present study shows that LDL-ICs up-regulate LDLR expression via the ERK signaling pathway and the AP-1 motif-dependent transcriptional activation...
  27. ncbi request reprint Chlamydophila pneumoniae infection of human aortic endothelial cells induces the expression of FC gamma receptor II (FcgammaRII)
    Silvana Vielma
    Department of Medicine, Medical University of South Carolina, and Ralph H Johnson VA Medical Center, Charleston, South Carolina 29425, USA
    Clin Immunol 104:265-73. 2002
    ..pneumoniae infection induces primarily the expression of FcgammaRII by endothelial cells and this may be a significant link between two proposed pathogenic mechanisms involved in the pathogenesis of human atherosclerosis...
  28. ncbi request reprint Markers of endothelial dysfunction in the prediction of coronary artery disease in type 1 diabetes. The Pittsburgh Epidemiology of Diabetes Complications Study
    Tina Costacou
    Department of Epidemiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA, USA
    J Diabetes Complications 19:183-93. 2005
    ..13, 95% Cl=1.03-1.24; HRs are per standard deviation increase) but not hard CAD. Study results suggest that E-selectin may enhance CAD prediction beyond traditional risk factors or markers of oxidative stress in Type 1 diabetes...
  29. ncbi request reprint Humoral immunity and atherosclerosis
    Gabriel Virella
    Nat Med 9:243-4; author reply 244-5. 2003
  30. ncbi request reprint Novel predictors of overt nephropathy in subjects with type 1 diabetes. A nested case control study from the Pittsburgh Epidemiology of Diabetes Complications cohort
    Aklilu A Yishak
    Department of Medicine, University of Pittsburgh School of Medicine, and Veterans Pittsburgh Health Care System of Pittsburgh, Pittsburgh, PA 15213, USA
    Nephrol Dial Transplant 21:93-100. 2006
    ..Predictors of diabetic nephropathy are only partly known and traditional risk factors do not adequately explain disease risk. We thus examined novel risk factors for overt nephropathy (ON) in type 1 diabetes...