G Virella

Summary

Affiliation: Medical University of South Carolina
Country: USA

Publications

  1. pmc Baseline markers of inflammation are associated with progression to macroalbuminuria in type 1 diabetic subjects
    Maria F Lopes-Virella
    Department of Medicine and Laboratory Services, Medical University of South Carolina and Ralph H Johnson VA Medical Center, Charleston, South Carolina, USA
    Diabetes Care 36:2317-23. 2013
  2. doi request reprint The levels of MDA-LDL in circulating immune complexes predict myocardial infarction in the VADT study
    Maria F Lopes-Virella
    Department of Medicine, Medical University of South Carolina, Charleston, SC, United States
    Atherosclerosis 224:526-31. 2012
  3. pmc High concentrations of AGE-LDL and oxidized LDL in circulating immune complexes are associated with progression of retinopathy in type 1 diabetes
    Maria F Lopes-Virella
    Department of Medicine, Medical University of South Carolina, Charleston, South Carolina, USA
    Diabetes Care 35:1333-40. 2012
  4. pmc High levels of oxidized LDL in circulating immune complexes are associated with increased odds of developing abnormal albuminuria in Type 1 diabetes
    Maria F Lopes-Virella
    Department of Medicine and Laboratory Services, Medical University of South Carolina and Ralph H Johnson VA Medical Center, Charleston, SC, USA
    Nephrol Dial Transplant 27:1416-23. 2012
  5. ncbi request reprint Role of simvastatin as an immunomodulator in type 2 diabetes
    Maria F Lopes-Virella
    Department of Medicine, Medical University of South Carolina, and Ralph H Johnson Department of Veterans Affairs Medical Center, Charleston, South Carolina 29425, USA
    Diabetes Care 27:908-13. 2004
  6. ncbi request reprint The preparation of copper-oxidized LDL for the measurement of oxidized LDL antibodies by EIA
    M F Lopes-Virella
    Division of Endocrinology, Diabetes and Medical Genetics, Department of Medicine, Ralph H Johnson VA Medical Center, Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC, USA
    Atherosclerosis 152:107-15. 2000
  7. ncbi request reprint Immune complexes containing modified lipoproteins are related to the progression of internal carotid intima-media thickness in patients with type 1 diabetes
    Maria F Lopes-Virella
    Ralph H Johnson Department of Veterans Affairs Medical Center, Charleston, SC, USA
    Atherosclerosis 190:359-69. 2007
  8. pmc Risk factors related to inflammation and endothelial dysfunction in the DCCT/EDIC cohort and their relationship with nephropathy and macrovascular complications
    Maria F Lopes-Virella
    Department of Medicine and Laboratory Services, Medical University of South Carolina and Ralph H Johnson VA Medical Center, Charleston, South Carolina, USA
    Diabetes Care 31:2006-12. 2008
  9. pmc Levels of oxidized LDL and advanced glycation end products-modified LDL in circulating immune complexes are strongly associated with increased levels of carotid intima-media thickness and its progression in type 1 diabetes
    Maria F Lopes-Virella
    Department of Medicine, Medical University of South Carolina, Charleston, South Carolina, USA
    Diabetes 60:582-9. 2011
  10. pmc Oxidized LDL immune complexes and coronary artery calcification in type 1 diabetes
    Maria F Lopes-Virella
    Department of Medicine, Medical University of South Carolina, Charleston, SC, United States
    Atherosclerosis 214:462-7. 2011

Collaborators

Detail Information

Publications29

  1. pmc Baseline markers of inflammation are associated with progression to macroalbuminuria in type 1 diabetic subjects
    Maria F Lopes-Virella
    Department of Medicine and Laboratory Services, Medical University of South Carolina and Ralph H Johnson VA Medical Center, Charleston, South Carolina, USA
    Diabetes Care 36:2317-23. 2013
    ....
  2. doi request reprint The levels of MDA-LDL in circulating immune complexes predict myocardial infarction in the VADT study
    Maria F Lopes-Virella
    Department of Medicine, Medical University of South Carolina, Charleston, SC, United States
    Atherosclerosis 224:526-31. 2012
    ..The present study was undertaken to determine whether the levels of oxidized LDL (oxLDL), malondialdehyde-LDL (MDA-LDL) and advanced glycation end products-LDL (AGE-LDL) in IC predict incident CVD events in type 2 diabetes (VADT cohort)...
  3. pmc High concentrations of AGE-LDL and oxidized LDL in circulating immune complexes are associated with progression of retinopathy in type 1 diabetes
    Maria F Lopes-Virella
    Department of Medicine, Medical University of South Carolina, Charleston, South Carolina, USA
    Diabetes Care 35:1333-40. 2012
    ..To determine whether immunocomplexes (ICs) containing advanced glycation end product (AGE)-LDL (AGE-LDL) and oxidized LDL (oxLDL) contribute to the development of retinopathy over a 16-year period in subjects with type 1 diabetes...
  4. pmc High levels of oxidized LDL in circulating immune complexes are associated with increased odds of developing abnormal albuminuria in Type 1 diabetes
    Maria F Lopes-Virella
    Department of Medicine and Laboratory Services, Medical University of South Carolina and Ralph H Johnson VA Medical Center, Charleston, SC, USA
    Nephrol Dial Transplant 27:1416-23. 2012
    ..Modified low-density lipoprotein (LDL) immune complexes (IC) have proinflammatory properties and play a role in albuminuria development...
  5. ncbi request reprint Role of simvastatin as an immunomodulator in type 2 diabetes
    Maria F Lopes-Virella
    Department of Medicine, Medical University of South Carolina, and Ralph H Johnson Department of Veterans Affairs Medical Center, Charleston, South Carolina 29425, USA
    Diabetes Care 27:908-13. 2004
    ....
  6. ncbi request reprint The preparation of copper-oxidized LDL for the measurement of oxidized LDL antibodies by EIA
    M F Lopes-Virella
    Division of Endocrinology, Diabetes and Medical Genetics, Department of Medicine, Ralph H Johnson VA Medical Center, Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC, USA
    Atherosclerosis 152:107-15. 2000
    ..The CV for 13 EIA runs of two reference oxLDL antibodies reacting with four different pools of standardized oxLDL prepared according to the stated guidelines was 14.5 and 3.9%, confirming the reproducibility of our oxidation conditions...
  7. ncbi request reprint Immune complexes containing modified lipoproteins are related to the progression of internal carotid intima-media thickness in patients with type 1 diabetes
    Maria F Lopes-Virella
    Ralph H Johnson Department of Veterans Affairs Medical Center, Charleston, SC, USA
    Atherosclerosis 190:359-69. 2007
    ..In conclusion these data demonstrate that increased levels of modified ApoB-rich IC are associated with increased progression of internal carotid IMT in the DCCT/EDIC cohort of type 1 diabetes...
  8. pmc Risk factors related to inflammation and endothelial dysfunction in the DCCT/EDIC cohort and their relationship with nephropathy and macrovascular complications
    Maria F Lopes-Virella
    Department of Medicine and Laboratory Services, Medical University of South Carolina and Ralph H Johnson VA Medical Center, Charleston, South Carolina, USA
    Diabetes Care 31:2006-12. 2008
    ....
  9. pmc Levels of oxidized LDL and advanced glycation end products-modified LDL in circulating immune complexes are strongly associated with increased levels of carotid intima-media thickness and its progression in type 1 diabetes
    Maria F Lopes-Virella
    Department of Medicine, Medical University of South Carolina, Charleston, South Carolina, USA
    Diabetes 60:582-9. 2011
    ....
  10. pmc Oxidized LDL immune complexes and coronary artery calcification in type 1 diabetes
    Maria F Lopes-Virella
    Department of Medicine, Medical University of South Carolina, Charleston, SC, United States
    Atherosclerosis 214:462-7. 2011
    ..We measured oxLDL in IC (oxLDL-IC) isolated from patients with type 1 diabetes to assess the relationship between oxLDL-IC and coronary artery calcification (CAC)...
  11. ncbi request reprint The role of immune and inflammatory processes in the development of macrovascular disease in diabetes
    Maria F Lopes-Virella
    Ralph H Jonhson VA Medical Center and Department of Medicine, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Front Biosci 8:s750-68. 2003
    ..These autoantibodies to modified lipoproteins have sufficient affinity to form stable antigen-antibody complexes, which have been also shown to have pro-atheromatous and pro-inflammatory properties...
  12. ncbi request reprint Chlamydophila pneumoniae induces ICAM-1 expression in human aortic endothelial cells via protein kinase C-dependent activation of nuclear factor-kappaB
    Silvana A Vielma
    Department of Microbiology and Immunology, Ralph H Johnson VA Medical Center and Medical University of South Carolina, Strom Thurmond Research Building, 114 Doughty St, Charleston, SC 29425, USA
    Circ Res 92:1130-7. 2003
    ..In conclusion, we demonstrated that C pneumoniae-induced ICAM-1 expression in HAECs requires NF-kappaB and PKC activation and that NF-kappaB activation is PKC dependent...
  13. ncbi request reprint Fc-gamma receptor cross-linking by immune complexes induces matrix metalloproteinase-1 in U937 cells via mitogen-activated protein kinase
    Y Huang
    Division of Endocrinology, Diabetes, and Medical Genetics, Department of Medicine, Medical University of South Carolina, Charleston, SC 29403, USA
    Arterioscler Thromb Vasc Biol 20:2533-8. 2000
    ..These results suggest that LDL-ICs and other ICs localized in atherosclerotic plaques may be potent stimulators for macrophage MMP-1 expression and may contribute to plaque rupture and acute coronary events...
  14. ncbi request reprint Oxidized LDL-anti-oxidized LDL immune complexes and diabetic nephropathy
    D H Atchley
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Diabetologia 45:1562-71. 2002
    ....
  15. ncbi request reprint Proatherogenic and proinflammatory properties of immune complexes prepared with purified human oxLDL antibodies and human oxLDL
    G Virella
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, South Carolina 29403, USA
    Clin Immunol 105:81-92. 2002
    ..This strongly supports the concept that modified LDL-IC present in circulation and/or tissues play an important pathogenic role in arteriosclerosis...
  16. pmc Modern-day clinical course of type 1 diabetes mellitus after 30 years' duration: the diabetes control and complications trial/epidemiology of diabetes interventions and complications and Pittsburgh epidemiology of diabetes complications experience (1983-2
    David M Nathan
    Diabetes Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114 2698, USA
    Arch Intern Med 169:1307-16. 2009
    ..There have been few longitudinal studies to describe the clinical course of T1DM in the age of intensive therapy. Our objective was to describe the current-day clinical course of T1DM...
  17. ncbi request reprint Antibody response to DT-GM, a novel fusion toxin consisting of a truncated diphtheria toxin (DT) linked to human granulocyte-macrophage colony stimulating factor (GM), during a phase I trial of patients with relapsed or refractory acute myeloid leukemia
    P D Hall
    College of Pharmacy, Hollings Cancer Center, Charleston, South Carolina 29425, USA
    Clin Immunol 100:191-7. 2001
    ..Further studies are needed to determine the clinical impact of the anti-DT-GM antibodies and whether the neutralization bioassay can be replaced by our EIA...
  18. pmc Lipoprotein autoantibodies: measurement and significance
    Gabriel Virella
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, 29425, USA
    Clin Diagn Lab Immunol 10:499-505. 2003
  19. ncbi request reprint Chlamydophila pneumoniae infection of human aortic endothelial cells induces the expression of FC gamma receptor II (FcgammaRII)
    Silvana Vielma
    Department of Medicine, Medical University of South Carolina, and Ralph H Johnson VA Medical Center, Charleston, South Carolina 29425, USA
    Clin Immunol 104:265-73. 2002
    ..pneumoniae infection induces primarily the expression of FcgammaRII by endothelial cells and this may be a significant link between two proposed pathogenic mechanisms involved in the pathogenesis of human atherosclerosis...
  20. ncbi request reprint Autoimmune response to advanced glycosylation end-products of human LDL
    Gabriel Virella
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC 92425, USA
    J Lipid Res 44:487-93. 2003
    ..A comparative study of the reactivity of purified AGE-LDL antibodies with CML-LDL and CML-serum albumin showed no cross-reactivity...
  21. pmc Distribution of IgM and IgG antibodies to oxidized LDL in immune complexes isolated from patients with type 1 diabetes and its relationship with nephropathy
    Gabriel Virella
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC 29425, USA
    Clin Immunol 127:394-400. 2008
    ..In conclusion, the predominance of pro-inflammatory IgG oxLDL antibodies is associated with existence of diabetic nephropathy, and a protective role of IgM antibodies could not be demonstrated...
  22. ncbi request reprint OxLDL immune complexes activate complement and induce cytokine production by MonoMac 6 cells and human macrophages
    Antonio F Saad
    Ralph H Johnson Department of Veterans Affairs Medical Center, Charleston, SC, USA
    J Lipid Res 47:1975-83. 2006
    ..In conclusion, human OxLDL-ICs have proinflammatory properties, as reflected by their capacity to activate the classical pathway of complement and to induce proinflammatory cytokine release from MM6 cells and primary human macrophages...
  23. ncbi request reprint Humoral immunity and atherosclerosis
    Gabriel Virella
    Nat Med 9:243-4; author reply 244-5. 2003
  24. ncbi request reprint Novel predictors of overt nephropathy in subjects with type 1 diabetes. A nested case control study from the Pittsburgh Epidemiology of Diabetes Complications cohort
    Aklilu A Yishak
    Department of Medicine, University of Pittsburgh School of Medicine, and Veterans Pittsburgh Health Care System of Pittsburgh, Pittsburgh, PA 15213, USA
    Nephrol Dial Transplant 21:93-100. 2006
    ..Predictors of diabetic nephropathy are only partly known and traditional risk factors do not adequately explain disease risk. We thus examined novel risk factors for overt nephropathy (ON) in type 1 diabetes...
  25. ncbi request reprint The immunogenicity of modified lipoproteins
    Maria F Lopes-Virella
    Strom Thurmond Research Building, 114 Doughty Street, Room 529, Charleston, SC 29425, USA
    Ann N Y Acad Sci 1043:367-78. 2005
    ..In conclusion, isolation and characterization of LDL-ICs appears to yield information of significant value that is not derived from other approaches to measure LDL modifications and their corresponding antibodies in humans...
  26. ncbi request reprint Markers of endothelial dysfunction in the prediction of coronary artery disease in type 1 diabetes. The Pittsburgh Epidemiology of Diabetes Complications Study
    Tina Costacou
    Department of Epidemiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA, USA
    J Diabetes Complications 19:183-93. 2005
    ..13, 95% Cl=1.03-1.24; HRs are per standard deviation increase) but not hard CAD. Study results suggest that E-selectin may enhance CAD prediction beyond traditional risk factors or markers of oxidative stress in Type 1 diabetes...
  27. ncbi request reprint Definition of the immunogenic forms of modified human LDL recognized by human autoantibodies and by rabbit hyperimmune antibodies
    Gabriel Virella
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC, USA
    J Lipid Res 45:1859-67. 2004
    ....
  28. ncbi request reprint LDL immune complexes stimulate LDL receptor expression in U937 histiocytes via extracellular signal-regulated kinase and AP-1
    Yuchang Fu
    Ralph H Johnson Veterans Administration Medical Center, Charleston, SC 29401, USA
    J Lipid Res 44:1315-21. 2003
    ..In conclusion, the present study shows that LDL-ICs up-regulate LDLR expression via the ERK signaling pathway and the AP-1 motif-dependent transcriptional activation...
  29. pmc Development of capture assays for different modifications of human low-density lipoprotein
    Gabriel Virella
    Medical University of South Carolina, Department of Microbiology and Immunology, 173 Ashley Ave, PO Box 250504, Charleston, SC 29425, USA
    Clin Diagn Lab Immunol 12:68-75. 2005
    ..This approach obviates the interference of IC in previously reported modified LDL assays and allows determination of the degree of modification of LDL with greater accuracy...

Research Grants10

  1. Biomarkers of Vascular Disease Progression in Type 1 Diabetes Mellitus
    Maria Lopes Virella; Fiscal Year: 2009
    ....
  2. Biomarkers of Vascular Disease Progression in Type 1 Diabetes Mellitus
    Maria Lopes Virella; Fiscal Year: 2009
    ....
  3. MACROPHAGE ACTIVATION AND LIPOPROTEIN METABOLISM
    Maria Lopes Virella; Fiscal Year: 2001
    ..Our overall objective is to provide insight into the mechanisms by which anti-LDL antibodies and macrophage activation may contribute to the development(acceleration of the arteriosclerotic process. ..
  4. Biomarkers of Vascular Disease Progression in Type 1 Diabetes Mellitus
    Maria F Lopes Virella; Fiscal Year: 2010
    ....