Research Topics
Species | G VirellaSummaryAffiliation: Medical University of South Carolina Country: USA Publications
Research Grants
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Publications
The levels of MDA-LDL in circulating immune complexes predict myocardial infarction in the VADT studyMaria F Lopes-Virella
Department of Medicine, Medical University of South Carolina, Charleston, SC, United States
Atherosclerosis 224:526-31. 2012..The present study was undertaken to determine whether the levels of oxidized LDL (oxLDL), malondialdehyde-LDL (MDA-LDL) and advanced glycation end products-LDL (AGE-LDL) in IC predict incident CVD events in type 2 diabetes (VADT cohort)...
High concentrations of AGE-LDL and oxidized LDL in circulating immune complexes are associated with progression of retinopathy in type 1 diabetesMaria F Lopes-Virella
Department of Medicine, Medical University of South Carolina, Charleston, South Carolina, USA
Diabetes Care 35:1333-40. 2012..To determine whether immunocomplexes (ICs) containing advanced glycation end product (AGE)-LDL (AGE-LDL) and oxidized LDL (oxLDL) contribute to the development of retinopathy over a 16-year period in subjects with type 1 diabetes...- High levels of oxidized LDL in circulating immune complexes are associated with increased odds of developing abnormal albuminuria in Type 1 diabetesMaria F Lopes-Virella
Department of Medicine and Laboratory Services, Medical University of South Carolina and Ralph H Johnson VA Medical Center, Charleston, SC, USA
Nephrol Dial Transplant 27:1416-23. 2012..Modified low-density lipoprotein (LDL) immune complexes (IC) have proinflammatory properties and play a role in albuminuria development... 
Role of simvastatin as an immunomodulator in type 2 diabetesMaria F Lopes-Virella
Department of Medicine, Medical University of South Carolina, and Ralph H Johnson Department of Veterans Affairs Medical Center, Charleston, South Carolina 29425, USA
Diabetes Care 27:908-13. 2004....- The preparation of copper-oxidized LDL for the measurement of oxidized LDL antibodies by EIAM F Lopes-Virella
Division of Endocrinology, Diabetes and Medical Genetics, Department of Medicine, Ralph H Johnson VA Medical Center, Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC, USA
Atherosclerosis 152:107-15. 2000..The CV for 13 EIA runs of two reference oxLDL antibodies reacting with four different pools of standardized oxLDL prepared according to the stated guidelines was 14.5 and 3.9%, confirming the reproducibility of our oxidation conditions... - Immune complexes containing modified lipoproteins are related to the progression of internal carotid intima-media thickness in patients with type 1 diabetesMaria F Lopes-Virella
Ralph H Johnson Department of Veterans Affairs Medical Center, Charleston, SC, USA
Atherosclerosis 190:359-69. 2007..In conclusion these data demonstrate that increased levels of modified ApoB-rich IC are associated with increased progression of internal carotid IMT in the DCCT/EDIC cohort of type 1 diabetes... - Risk factors related to inflammation and endothelial dysfunction in the DCCT/EDIC cohort and their relationship with nephropathy and macrovascular complicationsMaria F Lopes-Virella
Department of Medicine and Laboratory Services, Medical University of South Carolina and Ralph H Johnson VA Medical Center, Charleston, South Carolina, USA
Diabetes Care 31:2006-12. 2008.... - Levels of oxidized LDL and advanced glycation end products-modified LDL in circulating immune complexes are strongly associated with increased levels of carotid intima-media thickness and its progression in type 1 diabetesMaria F Lopes-Virella
Department of Medicine, Medical University of South Carolina, Charleston, South Carolina, USA
Diabetes 60:582-9. 2011.... - Oxidized LDL immune complexes and coronary artery calcification in type 1 diabetesMaria F Lopes-Virella
Department of Medicine, Medical University of South Carolina, Charleston, SC, United States
Atherosclerosis 214:462-7. 2011..We measured oxLDL in IC (oxLDL-IC) isolated from patients with type 1 diabetes to assess the relationship between oxLDL-IC and coronary artery calcification (CAC)... - The role of immune and inflammatory processes in the development of macrovascular disease in diabetesMaria F Lopes-Virella
Ralph H Jonhson VA Medical Center and Department of Medicine, Medical University of South Carolina, Charleston, South Carolina 29425, USA
Front Biosci 8:s750-68. 2003..These autoantibodies to modified lipoproteins have sufficient affinity to form stable antigen-antibody complexes, which have been also shown to have pro-atheromatous and pro-inflammatory properties... - Chlamydophila pneumoniae induces ICAM-1 expression in human aortic endothelial cells via protein kinase C-dependent activation of nuclear factor-kappaBSilvana A Vielma
Department of Microbiology and Immunology, Ralph H Johnson VA Medical Center and Medical University of South Carolina, Strom Thurmond Research Building, 114 Doughty St, Charleston, SC 29425, USA
Circ Res 92:1130-7. 2003..In conclusion, we demonstrated that C pneumoniae-induced ICAM-1 expression in HAECs requires NF-kappaB and PKC activation and that NF-kappaB activation is PKC dependent... - Fc-gamma receptor cross-linking by immune complexes induces matrix metalloproteinase-1 in U937 cells via mitogen-activated protein kinaseY Huang
Division of Endocrinology, Diabetes, and Medical Genetics, Department of Medicine, Medical University of South Carolina, Charleston, SC 29403, USA
Arterioscler Thromb Vasc Biol 20:2533-8. 2000..These results suggest that LDL-ICs and other ICs localized in atherosclerotic plaques may be potent stimulators for macrophage MMP-1 expression and may contribute to plaque rupture and acute coronary events... - Oxidized LDL-anti-oxidized LDL immune complexes and diabetic nephropathyD H Atchley
Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
Diabetologia 45:1562-71. 2002..05). CONCLUSION/INTERPRETATION: The data indicate that high concentrations of oxidized LDL-anti-oxidized LDL complexes, particularly when formed with antibodies of higher avidity, are associated with abnormal proteinuria... - Proatherogenic and proinflammatory properties of immune complexes prepared with purified human oxLDL antibodies and human oxLDLG Virella
Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, South Carolina 29403, USA
Clin Immunol 105:81-92. 2002..This strongly supports the concept that modified LDL-IC present in circulation and/or tissues play an important pathogenic role in arteriosclerosis... - Modern-day clinical course of type 1 diabetes mellitus after 30 years' duration: the diabetes control and complications trial/epidemiology of diabetes interventions and complications and Pittsburgh epidemiology of diabetes complications experience (1983-2David M Nathan
Diabetes Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114 2698, USA
Arch Intern Med 169:1307-16. 2009..There have been few longitudinal studies to describe the clinical course of T1DM in the age of intensive therapy. Our objective was to describe the current-day clinical course of T1DM... - Antibody response to DT-GM, a novel fusion toxin consisting of a truncated diphtheria toxin (DT) linked to human granulocyte-macrophage colony stimulating factor (GM), during a phase I trial of patients with relapsed or refractory acute myeloid leukemiaP D Hall
College of Pharmacy, Hollings Cancer Center, Charleston, South Carolina 29425, USA
Clin Immunol 100:191-7. 2001..Further studies are needed to determine the clinical impact of the anti-DT-GM antibodies and whether the neutralization bioassay can be replaced by our EIA... - Lipoprotein autoantibodies: measurement and significanceGabriel Virella
Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, 29425, USA
Clin Diagn Lab Immunol 10:499-505. 2003 - Chlamydophila pneumoniae infection of human aortic endothelial cells induces the expression of FC gamma receptor II (FcgammaRII)Silvana Vielma
Department of Medicine, Medical University of South Carolina, and Ralph H. Johnson VA Medical Center, Charleston, South Carolina 29425, USA
Clin Immunol 104:265-73. 2002..pneumoniae infection induces primarily the expression of FcgammaRII by endothelial cells and this may be a significant link between two proposed pathogenic mechanisms involved in the pathogenesis of human atherosclerosis... - Distribution of IgM and IgG antibodies to oxidized LDL in immune complexes isolated from patients with type 1 diabetes and its relationship with nephropathyGabriel Virella
Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC 29425, USA
Clin Immunol 127:394-400. 2008..In conclusion, the predominance of pro-inflammatory IgG oxLDL antibodies is associated with existence of diabetic nephropathy, and a protective role of IgM antibodies could not be demonstrated... - OxLDL immune complexes activate complement and induce cytokine production by MonoMac 6 cells and human macrophagesAntonio F Saad
Ralph H. Johnson Department of Veterans Affairs Medical Center, Charleston, SC, USA
J Lipid Res 47:1975-83. 2006..In conclusion, human OxLDL-ICs have proinflammatory properties, as reflected by their capacity to activate the classical pathway of complement and to induce proinflammatory cytokine release from MM6 cells and primary human macrophages... - Autoimmune response to advanced glycosylation end-products of human LDLGabriel Virella
Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC 92425, USA
J Lipid Res 44:487-93. 2003..A comparative study of the reactivity of purified AGE-LDL antibodies with CML-LDL and CML-serum albumin showed no cross-reactivity... - Novel predictors of overt nephropathy in subjects with type 1 diabetes. A nested case control study from the Pittsburgh Epidemiology of Diabetes Complications cohortAklilu A Yishak
Department of Medicine, University of Pittsburgh School of Medicine, and Veterans Pittsburgh Health Care System of Pittsburgh, Pittsburgh, PA 15213, USA
Nephrol Dial Transplant 21:93-100. 2006..CONCLUSION: Besides AER, the immunecomplexes and lipoprotein subclasses may provide additional information in the assessment of ON risk in type 1 diabetes... - The immunogenicity of modified lipoproteinsMaria F Lopes-Virella
Strom Thurmond Research Building, 114 Doughty Street, Room 529, Charleston, SC 29425, USA
Ann N Y Acad Sci 1043:367-78. 2005..In conclusion, isolation and characterization of LDL-ICs appears to yield information of significant value that is not derived from other approaches to measure LDL modifications and their corresponding antibodies in humans... - Markers of endothelial dysfunction in the prediction of coronary artery disease in type 1 diabetes. The Pittsburgh Epidemiology of Diabetes Complications StudyTina Costacou
Department of Epidemiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA, USA
J Diabetes Complications 19:183-93. 2005..13, 95% Cl=1.03-1.24; HRs are per standard deviation increase) but not hard CAD. Study results suggest that E-selectin may enhance CAD prediction beyond traditional risk factors or markers of oxidative stress in Type 1 diabetes... - Humoral immunity and atherosclerosisGabriel Virella
Nat Med 9:243-4; author reply 244-5. 2003 - Definition of the immunogenic forms of modified human LDL recognized by human autoantibodies and by rabbit hyperimmune antibodiesGabriel Virella
Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC, USA
J Lipid Res 45:1859-67. 2004.... - LDL immune complexes stimulate LDL receptor expression in U937 histiocytes via extracellular signal-regulated kinase and AP-1Yuchang Fu
Ralph H. Johnson Veterans Administration Medical Center, Charleston, SC 29401, USA
J Lipid Res 44:1315-21. 2003..In conclusion, the present study shows that LDL-ICs up-regulate LDLR expression via the ERK signaling pathway and the AP-1 motif-dependent transcriptional activation... - Development of capture assays for different modifications of human low-density lipoproteinGabriel Virella
Medical University of South Carolina, Department of Microbiology and Immunology, 173 Ashley Ave, PO Box 250504, Charleston, SC 29425, USA
Clin Diagn Lab Immunol 12:68-75. 2005..This approach obviates the interference of IC in previously reported modified LDL assays and allows determination of the degree of modification of LDL with greater accuracy...
Research Grants
- Biomarkers of Vascular Disease Progression in Type 1 Diabetes MellitusMaria Lopes Virella; Fiscal Year: 2009....
- Biomarkers of Vascular Disease Progression in Type 1 Diabetes MellitusMaria Lopes Virella; Fiscal Year: 2009....
- MACROPHAGE ACTIVATION AND LIPOPROTEIN METABOLISMMaria Lopes Virella; Fiscal Year: 2001..Our overall objective is to provide insight into the mechanisms by which anti-LDL antibodies and macrophage activation may contribute to the development(acceleration of the arteriosclerotic process. ..
- Biomarkers of Vascular Disease Progression in Type 1 Diabetes MellitusMaria F Lopes Virella; Fiscal Year: 2010....