Research Topics
| WITOLD SUBCZYNSKISummaryAffiliation: Medical College of Wisconsin Country: USA Publications
Research Grants
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Detail Information
Publications
Functions of cholesterol and the cholesterol bilayer domain specific to the fiber-cell plasma membrane of the eye lensWitold K Subczynski
Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
J Membr Biol 245:51-68. 2012..In this review, we provide data supporting these hypotheses...- Location of macular xanthophylls in the most vulnerable regions of photoreceptor outer-segment membranesWitold K Subczynski
Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
Arch Biochem Biophys 504:61-6. 2010.... - Physical properties of lipid bilayers from EPR spin labeling and their influence on chemical reactions in a membrane environmentWitold K Subczynski
Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
Free Radic Biol Med 46:707-18. 2009..These properties can drastically vary with membrane composition, membrane depth, and membrane domain formation, influencing the fate of chemical reactions that occur in a lipid bilayer environment... 
Molecular organization and dynamics of 1-palmitoyl-2-oleoylphosphatidylcholine bilayers containing a transmembrane alpha-helical peptideW K Subczynski
Biophysics Department, Institute of Molecular Biology, Jagiellonian University, Al Mickiewicza 3, 31 120 Krakow, Poland
Biochemistry 37:3156-64. 1998..It is concluded that L24 is highly miscible in POPC membranes even at high concentrations in the membrane...- Three-dimensional dynamic structure of the liquid-ordered domain in lipid membranes as examined by pulse-EPR oxygen probingWitold K Subczynski
Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
Biophys J 92:1573-84. 2007..Third, the transmembrane OTP profile in the liquid-ordered domain that contained 50 mol % cholesterol dramatically differed from that which contained 27 mol % cholesterol... - Effects of pH-induced variations of the charge of the transmembrane alpha-helical peptide Ac-K2(LA)12K2-amide on the organization and dynamics of the host dimyristoylphosphatidylcholine bilayer membraneWitold K Subczynski
Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, 53226, USA
Biochim Biophys Acta 1720:99-109. 2005..It does not affect the local diffusion-solubility product of oxygen measured in the DMPC-(LA)(12) membrane interior... - Molecular dynamics of 1-palmitoyl-2-oleoylphosphatidylcholine membranes containing transmembrane alpha-helical peptides with alternating leucine and alanine residuesWitold K Subczynski
Biophysics Research Institute, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
Biochemistry 42:3939-48. 2003..This explanation was confirmed by calculating the orientational order of leucine side chains in (LA)(12) and L(24) from molecular dynamics simulation studies... 
Studying lipid organization in biological membranes using liposomes and EPR spin labelingWitold K Subczynski
Department of Biophysics, Medical College of Wisconsin, Milwaukee, USA
Methods Mol Biol 606:247-69. 2010..To better elucidate the major factors that determine membrane properties, results for eye lens lipid membranes and simple model membranes that resemble the basic lipid composition of biological membranes will be compared...- Physical properties of the lipid bilayer membrane made of calf lens lipids: EPR spin labeling studiesJustyna Widomska
Department of Biophysics, Medical College of Wisconsin, Milwaukee, 8701 Watertown Plank Road, WI 53226, USA
Biochim Biophys Acta 1768:1454-65. 2007..These profiles in POPC membranes are bell-shaped. It is concluded that the high level of cholesterol in lens lipids makes the membrane stable, immobile, and impermeable to both polar and nonpolar molecules... - Dynamics of raft molecules in the cell and artificial membranes: approaches by pulse EPR spin labeling and single molecule optical microscopyWitold K Subczynski
National Biomedical EPR Center, Biophysics Research Institute, The Medical College of Wisconsin, Milwaukee, WI 53226, USA
Biochim Biophys Acta 1610:231-43. 2003..Finally, we propose a thermal Lego model for rafts as the basic building blocks for signaling pathways in the plasma membrane... - Characterization of lipid domains in reconstituted porcine lens membranes using EPR spin-labeling approachesMarija Raguz
Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
Biochim Biophys Acta 1778:1079-90. 2008..This work was undertaken to better elucidate the major factors that determine membrane resistance to oxygen transport across the lens lipid membrane, with special attention paid to the cholesterol crystalline domain... - Saturation-recovery electron paramagnetic resonance discrimination by oxygen transport (DOT) method for characterizing membrane domainsWitold K Subczynski
Dept of Biophysics, Medical College of Wisconsin, Milwaukee 53226, USA
Methods Mol Biol 398:143-57. 2007..Furthermore, under optimal conditions, the exchange rate of spin-labeled molecules between the two domains could be measured... - Transmembrane localization of cis-isomers of zeaxanthin in the host dimyristoylphosphatidylcholine bilayer membraneJustyna Widomska
Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
Biochim Biophys Acta 1778:10-9. 2008..However, the existence of the second pool of cis-zeaxanthin molecules located in the one leaflet and anchored by the terminal hydroxyl groups in the same polar headgroup region cannot be completely ruled out... - Oxygen permeability of the lipid bilayer membrane made of calf lens lipidsJustyna Widomska
Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
Biochim Biophys Acta 1768:2635-45. 2007..In the central region of the membrane, oxygen transport is enhanced, significantly exceeding that in bulk water. It is concluded that the high level of cholesterol in lens lipids is responsible for these unique membrane properties... - Is a fluid-mosaic model of biological membranes fully relevant? Studies on lipid organization in model and biological membranesAnna Wisniewska
Biophysics Department, Institute of Molecular Biology and Biotechnology, Jagiellonian University, Krakow, Poland
Cell Mol Biol Lett 8:147-59. 2003..Conventional and pulse EPR (electron paramagnetic resonance) spin labeling techniques were employed to assess the molecular organization and dynamics of the raft-constituent molecules and of the raft itself in the membrane... - Carotenoid-membrane interactions in liposomes: effect of dipolar, monopolar, and nonpolar carotenoidsAnna Wisniewska
Department of Biophysics, Medical College of Wisconsin, Milwaukee, USA
Acta Biochim Pol 53:475-84. 2006..This manuscript also shows the ability of EPR spin-labeling methods to monitor different membrane properties that can be applied in biotechnological studies with the use of liposomes... - Saturation recovery EPR and ELDOR at W-band for spin labelsWojciech Froncisz
Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, P O Box 26509, Milwaukee, WI 53226 0509, USA
J Magn Reson 193:297-304. 2008..K. Subczynski, T.G. Camenisch, J.J. Ratke, W. Froncisz, Spin label EPR T(1) values using saturation recovery from 2 to 35 GHz. J. Phys. Chem. B 108 (2004) 9524-9529]. The values of T(1e) decrease at 94 GHz relative to values at 35 GHz... - Accumulation of macular xanthophylls in unsaturated membrane domainsAnna Wisniewska
Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
Free Radic Biol Med 40:1820-6. 2006.... - Structures of HO(2)-Co(III)bleomycin A(2) bound to d(GAGCTC)(2) and d(GGAAGCTTCC)(2): structure-reactivity relationships of Co and Fe bleomycinsChunqing Zhao
Department of Chemistry, University of Wisconsin-Milwaukee, Milwaukee, WI 53201, USA
J Inorg Biochem 91:259-68. 2002..They also rationalize the instability of a number of ligands bound to Co(III)/Fe(III)Blm at specific binding sequences and the relative unreactivity of Fe(III)Blm[bond]I with ascorbate as well as its lack of interaction with spin labels... - Concentration by centrifugation for gas exchange EPR oximetry measurements with loop-gap resonatorsWitold K Subczynski
Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
J Magn Reson 176:244-8. 2005..Typically, the SNR was improved by a factor of five to eight. Oxygen accessibility applications in site-directed spin labeling studies are discussed...
Research Grants
- Lipid Rafts in Eye Lens: Discrimination by Pulse EPRWITOLD SUBCZYNSKI; Fiscal Year: 2009..Age-related nuclear cataract is a primary cause of blindness in the elderly in third world countries. ..
- Cholesterol Crystalline Domain Function in Eye Lens: EPR Spin-Labeling StudiesWitold K Subczynski; Fiscal Year: 2010....
- Cholesterol Crystalline Domain Function in Eye Lens: EPR Spin-Labeling StudiesWitold K Subczynski; Fiscal Year: 2011....