WITOLD SUBCZYNSKI

Summary

Affiliation: Medical College of Wisconsin
Country: USA

Publications

  1. pmc Functions of cholesterol and the cholesterol bilayer domain specific to the fiber-cell plasma membrane of the eye lens
    Witold K Subczynski
    Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    J Membr Biol 245:51-68. 2012
  2. pmc Location of macular xanthophylls in the most vulnerable regions of photoreceptor outer-segment membranes
    Witold K Subczynski
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Arch Biochem Biophys 504:61-6. 2010
  3. pmc Physical properties of lipid bilayers from EPR spin labeling and their influence on chemical reactions in a membrane environment
    Witold K Subczynski
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 46:707-18. 2009
  4. ncbi request reprint Molecular organization and dynamics of 1-palmitoyl-2-oleoylphosphatidylcholine bilayers containing a transmembrane alpha-helical peptide
    W K Subczynski
    Biophysics Department, Institute of Molecular Biology, Jagiellonian University, Al Mickiewicza 3, 31 120 Krakow, Poland
    Biochemistry 37:3156-64. 1998
  5. pmc Three-dimensional dynamic structure of the liquid-ordered domain in lipid membranes as examined by pulse-EPR oxygen probing
    Witold K Subczynski
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Biophys J 92:1573-84. 2007
  6. ncbi request reprint Effects of pH-induced variations of the charge of the transmembrane alpha-helical peptide Ac-K2(LA)12K2-amide on the organization and dynamics of the host dimyristoylphosphatidylcholine bilayer membrane
    Witold K Subczynski
    Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, 53226, USA
    Biochim Biophys Acta 1720:99-109. 2005
  7. ncbi request reprint Molecular dynamics of 1-palmitoyl-2-oleoylphosphatidylcholine membranes containing transmembrane alpha-helical peptides with alternating leucine and alanine residues
    Witold K Subczynski
    Biophysics Research Institute, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Biochemistry 42:3939-48. 2003
  8. doi request reprint Studying lipid organization in biological membranes using liposomes and EPR spin labeling
    Witold K Subczynski
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, USA
    Methods Mol Biol 606:247-69. 2010
  9. pmc Physical properties of the lipid bilayer membrane made of calf lens lipids: EPR spin labeling studies
    Justyna Widomska
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, 8701 Watertown Plank Road, WI 53226, USA
    Biochim Biophys Acta 1768:1454-65. 2007
  10. ncbi request reprint Dynamics of raft molecules in the cell and artificial membranes: approaches by pulse EPR spin labeling and single molecule optical microscopy
    Witold K Subczynski
    National Biomedical EPR Center, Biophysics Research Institute, The Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Biochim Biophys Acta 1610:231-43. 2003

Collaborators

Detail Information

Publications20

  1. pmc Functions of cholesterol and the cholesterol bilayer domain specific to the fiber-cell plasma membrane of the eye lens
    Witold K Subczynski
    Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    J Membr Biol 245:51-68. 2012
    ..In this review, we provide data supporting these hypotheses...
  2. pmc Location of macular xanthophylls in the most vulnerable regions of photoreceptor outer-segment membranes
    Witold K Subczynski
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Arch Biochem Biophys 504:61-6. 2010
    ....
  3. pmc Physical properties of lipid bilayers from EPR spin labeling and their influence on chemical reactions in a membrane environment
    Witold K Subczynski
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 46:707-18. 2009
    ..These properties can drastically vary with membrane composition, membrane depth, and membrane domain formation, influencing the fate of chemical reactions that occur in a lipid bilayer environment...
  4. ncbi request reprint Molecular organization and dynamics of 1-palmitoyl-2-oleoylphosphatidylcholine bilayers containing a transmembrane alpha-helical peptide
    W K Subczynski
    Biophysics Department, Institute of Molecular Biology, Jagiellonian University, Al Mickiewicza 3, 31 120 Krakow, Poland
    Biochemistry 37:3156-64. 1998
    ..It is concluded that L24 is highly miscible in POPC membranes even at high concentrations in the membrane...
  5. pmc Three-dimensional dynamic structure of the liquid-ordered domain in lipid membranes as examined by pulse-EPR oxygen probing
    Witold K Subczynski
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Biophys J 92:1573-84. 2007
    ..Third, the transmembrane OTP profile in the liquid-ordered domain that contained 50 mol % cholesterol dramatically differed from that which contained 27 mol % cholesterol...
  6. ncbi request reprint Effects of pH-induced variations of the charge of the transmembrane alpha-helical peptide Ac-K2(LA)12K2-amide on the organization and dynamics of the host dimyristoylphosphatidylcholine bilayer membrane
    Witold K Subczynski
    Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, 53226, USA
    Biochim Biophys Acta 1720:99-109. 2005
    ..It does not affect the local diffusion-solubility product of oxygen measured in the DMPC-(LA)(12) membrane interior...
  7. ncbi request reprint Molecular dynamics of 1-palmitoyl-2-oleoylphosphatidylcholine membranes containing transmembrane alpha-helical peptides with alternating leucine and alanine residues
    Witold K Subczynski
    Biophysics Research Institute, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Biochemistry 42:3939-48. 2003
    ..This explanation was confirmed by calculating the orientational order of leucine side chains in (LA)(12) and L(24) from molecular dynamics simulation studies...
  8. doi request reprint Studying lipid organization in biological membranes using liposomes and EPR spin labeling
    Witold K Subczynski
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, USA
    Methods Mol Biol 606:247-69. 2010
    ..To better elucidate the major factors that determine membrane properties, results for eye lens lipid membranes and simple model membranes that resemble the basic lipid composition of biological membranes will be compared...
  9. pmc Physical properties of the lipid bilayer membrane made of calf lens lipids: EPR spin labeling studies
    Justyna Widomska
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, 8701 Watertown Plank Road, WI 53226, USA
    Biochim Biophys Acta 1768:1454-65. 2007
    ..These profiles in POPC membranes are bell-shaped. It is concluded that the high level of cholesterol in lens lipids makes the membrane stable, immobile, and impermeable to both polar and nonpolar molecules...
  10. ncbi request reprint Dynamics of raft molecules in the cell and artificial membranes: approaches by pulse EPR spin labeling and single molecule optical microscopy
    Witold K Subczynski
    National Biomedical EPR Center, Biophysics Research Institute, The Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Biochim Biophys Acta 1610:231-43. 2003
    ..Finally, we propose a thermal Lego model for rafts as the basic building blocks for signaling pathways in the plasma membrane...
  11. pmc Characterization of lipid domains in reconstituted porcine lens membranes using EPR spin-labeling approaches
    Marija Raguz
    Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Biochim Biophys Acta 1778:1079-90. 2008
    ..This work was undertaken to better elucidate the major factors that determine membrane resistance to oxygen transport across the lens lipid membrane, with special attention paid to the cholesterol crystalline domain...
  12. ncbi request reprint Saturation-recovery electron paramagnetic resonance discrimination by oxygen transport (DOT) method for characterizing membrane domains
    Witold K Subczynski
    Dept of Biophysics, Medical College of Wisconsin, Milwaukee 53226, USA
    Methods Mol Biol 398:143-57. 2007
    ..Furthermore, under optimal conditions, the exchange rate of spin-labeled molecules between the two domains could be measured...
  13. pmc Transmembrane localization of cis-isomers of zeaxanthin in the host dimyristoylphosphatidylcholine bilayer membrane
    Justyna Widomska
    Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Biochim Biophys Acta 1778:10-9. 2008
    ..However, the existence of the second pool of cis-zeaxanthin molecules located in the one leaflet and anchored by the terminal hydroxyl groups in the same polar headgroup region cannot be completely ruled out...
  14. pmc Oxygen permeability of the lipid bilayer membrane made of calf lens lipids
    Justyna Widomska
    Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Biochim Biophys Acta 1768:2635-45. 2007
    ..In the central region of the membrane, oxygen transport is enhanced, significantly exceeding that in bulk water. It is concluded that the high level of cholesterol in lens lipids is responsible for these unique membrane properties...
  15. ncbi request reprint Is a fluid-mosaic model of biological membranes fully relevant? Studies on lipid organization in model and biological membranes
    Anna Wisniewska
    Biophysics Department, Institute of Molecular Biology and Biotechnology, Jagiellonian University, Krakow, Poland
    Cell Mol Biol Lett 8:147-59. 2003
    ..Conventional and pulse EPR (electron paramagnetic resonance) spin labeling techniques were employed to assess the molecular organization and dynamics of the raft-constituent molecules and of the raft itself in the membrane...
  16. ncbi request reprint Carotenoid-membrane interactions in liposomes: effect of dipolar, monopolar, and nonpolar carotenoids
    Anna Wisniewska
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, USA
    Acta Biochim Pol 53:475-84. 2006
    ..This manuscript also shows the ability of EPR spin-labeling methods to monitor different membrane properties that can be applied in biotechnological studies with the use of liposomes...
  17. pmc Saturation recovery EPR and ELDOR at W-band for spin labels
    Wojciech Froncisz
    Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, P O Box 26509, Milwaukee, WI 53226 0509, USA
    J Magn Reson 193:297-304. 2008
    ..K. Subczynski, T.G. Camenisch, J.J. Ratke, W. Froncisz, Spin label EPR T(1) values using saturation recovery from 2 to 35 GHz. J. Phys. Chem. B 108 (2004) 9524-9529]. The values of T(1e) decrease at 94 GHz relative to values at 35 GHz...
  18. ncbi request reprint Accumulation of macular xanthophylls in unsaturated membrane domains
    Anna Wisniewska
    Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Free Radic Biol Med 40:1820-6. 2006
    ....
  19. ncbi request reprint Structures of HO(2)-Co(III)bleomycin A(2) bound to d(GAGCTC)(2) and d(GGAAGCTTCC)(2): structure-reactivity relationships of Co and Fe bleomycins
    Chunqing Zhao
    Department of Chemistry, University of Wisconsin Milwaukee, Milwaukee, WI 53201, USA
    J Inorg Biochem 91:259-68. 2002
    ..They also rationalize the instability of a number of ligands bound to Co(III)/Fe(III)Blm at specific binding sequences and the relative unreactivity of Fe(III)Blm[bond]I with ascorbate as well as its lack of interaction with spin labels...
  20. ncbi request reprint Concentration by centrifugation for gas exchange EPR oximetry measurements with loop-gap resonators
    Witold K Subczynski
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Magn Reson 176:244-8. 2005
    ..Typically, the SNR was improved by a factor of five to eight. Oxygen accessibility applications in site-directed spin labeling studies are discussed...

Research Grants6

  1. Lipid Rafts in Eye Lens: Discrimination by Pulse EPR
    WITOLD SUBCZYNSKI; Fiscal Year: 2009
    ..Age-related nuclear cataract is a primary cause of blindness in the elderly in third world countries. ..
  2. Cholesterol Crystalline Domain Function in Eye Lens: EPR Spin-Labeling Studies
    Witold K Subczynski; Fiscal Year: 2010
    ....
  3. Cholesterol Crystalline Domain Function in Eye Lens: EPR Spin-Labeling Studies
    Witold K Subczynski; Fiscal Year: 2011
    ....