Research Topics
Genomes and Genes | A SorokinSummaryAffiliation: Medical College of Wisconsin Country: USA Publications
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Publications
20-HETE-producing enzymes are up-regulated in human cancersAnna Alexanian
Kidney Disease Center, Department of Medicine and Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
Cancer Genomics Proteomics 9:163-9. 2012....
Endothelin signaling and actions in the renal mesangiumAndrey Sorokin
Kidney Disease Center, Division of Nephrology, Department of Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
Contrib Nephrol 172:50-62. 2011..Plentiful evidence suggests an essential role of ET-1-signaling and action in the renal mesangium for renal biology and pathobiology...
Protein kinase A-dependent phosphorylation modulates beta1Pix guanine nucleotide exchange factor activity through 14-3-3beta bindingAhmed Chahdi
Division of Nephrology and Kidney Disease Center, Department of Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
Mol Cell Biol 28:1679-87. 2008..These results indicate that 14-3-3beta negatively affects the GEF activity of dimeric beta(1)Pix only. Altogether, these results provide a mechanistic insight into the role of 14-3-3beta in modulating beta(1)Pix-GEF activity...- Protein-tyrosine kinase Pyk2 mediates endothelin-induced p38 MAPK activation in glomerular mesangial cellsA Sorokin
Department of Medicine, Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
J Biol Chem 276:21521-8. 2001..These findings suggest that the engagement of Pyk2 is important for ET-1-mediated p38 MAPK activation and hence the biological effect of this peptide in mesangial cells... - Cyclooxygenase-2: potential role in regulation of drug efflux and multidrug resistance phenotypeA Sorokin
Department of Medicine, Division of Nephrology and Cardiovascular Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
Curr Pharm Des 10:647-57. 2004..The use of Cox-2 inhibitors to decrease function of MDR1 may enhance accumulation of chemotherapy agents and decrease resistance of tumors to chemotherapeutic drugs... - Endothelin signalling and regulation of protein kinases in glomerular mesangial cellsAndrey Sorokin
Department of Medicine, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, U S A
Clin Sci (Lond) 103:132S-136S. 2002..Thus, p38 MAP kinase stimulation could perhaps partially account for ET-1 contractive properties, whereas ET-1-induced cell proliferation occurs primarily via Ras-dependent activation of the ERK... - Insulin stimulates the tyrosine dephosphorylation of docking protein p130cas (Crk-associated substrate), promoting the switch of the adaptor protein crk from p130cas to newly phosphorylated insulin receptor substrate-1A Sorokin
Department of Medicine and Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
Biochem J 334:595-600. 1998..Tyrosine-phosphorylated insulin receptor substrate-1 serves as a docking protein for multiple adaptor proteins and competes with p130(cas) for CrkII... - Endothelin 1 induces beta 1Pix translocation and Cdc42 activation via protein kinase A-dependent pathwayAhmed Chahdi
Department of Medicine, Division of Nephrology, Kidney Disease Center and Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
J Biol Chem 280:578-84. 2005..Our results have provided evidence that stimulation of PKA pathway by ET-1 or cAMP analog results in beta(1)Pix phosphorylation, which in turn controls beta(1)Pix translocation to focal complexes and Cdc42 activation... - PreproEndothelin-1 expression in human mesangial cells: evidence for a p38 mitogen-activated protein kinase/protein kinases-C-dependent mechanismM Foschi
Medical College of Wisconsin, Milwaukee, Wisconsin 53226-0509, USA
J Am Soc Nephrol 12:1137-50. 2001..In conclusion, human mesangial cell expression of preproET-1 may be increased potently in the presence of two common proinflammatory mediators, thereby providing a potential mechanism for ET-1 production in inflammatory renal disease... - CrkIII: a novel and biologically distinct member of the Crk family of adaptor proteinsSimon Prosser
Department of Medicine and Cardiovascular Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
Oncogene 22:4799-806. 2003.... - Alterations in subcellular localization of p38 MAPK potentiates endothelin-stimulated COX-2 expression in glomerular mesangial cellsPhillip F Pratt
Department of Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
J Biol Chem 278:51928-36. 2003..These results indicate that MKP-1 demonstrates a relative selectivity for p38 alpha MAPK versus p38 gamma MAPK in GMC and is likely to indirectly regulate the expression of COX-2... - Different activation of mitogen-activated protein kinases in experimental proliferative glomerulonephritisD Bokemeyer
Department of Medicine, Medical College of Wisconsin, Milwaukee, USA
Kidney Int Suppl 67:S189-91. 1998.... - RGS3 inhibits G protein-mediated signaling via translocation to the membrane and binding to Galpha11N O Dulin
Department of Medicine and Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
Mol Cell Biol 19:714-23. 1999..These data indicate that RGS3 inhibits G protein-coupled receptor signaling by a complex mechanism involving its translocation to the membrane in addition to its established function as a GTPase-activating protein... - Endothelin-1 couples betaPix to p66Shc: role of betaPix in cell proliferation through FOXO3a phosphorylation and p27kip1 down-regulation independently of AktAhmed Chahdi
Kidney Disease Center, Department of Medicine, Division of Nephrology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
Mol Biol Cell 19:2609-19. 2008..Our results identify p66Shc and FOXO3a as novel partners of beta(1)Pix and represent the first direct evidence of beta(1)Pix in cell proliferation via Erk/p66Shc-dependent and Akt-independent mechanisms... - Crk protein binds to PDGF receptor and insulin receptor substrate-1 with different modulating effects on PDGF- and insulin-dependent signaling pathwaysA Sorokin
Department of Medicine, Medical College of Wisconsin, Milwaukee 53226 0509, USA
Oncogene 16:2425-34. 1998..These data provide support to the hypothesis that insulin and PDGF employ different mechanisms for activation of MAP kinase cascade... - Regulation of TGF-beta1/MAPK-mediated PAI-1 gene expression by the actin cytoskeleton in human mesangial cellsChen Yang
Department of Pathology and Anatomy, Eastern Virginia Medical School, Norfolk, VA 23501, USA
Exp Cell Res 313:1240-50. 2007..Therefore, changes in actin cytoskeleton modulate the ability of TGF-beta1 to stimulate PAI-1 expression through a mechanism dependent on the activation of MAPK/AP-1 pathways... - The Rac/Cdc42 guanine nucleotide exchange factor beta1Pix enhances mastoparan-activated Gi-dependent pathway in mast cellsAhmed Chahdi
Division of Nephrology and the Cardiovascular Research Center, Department of Medicine, Medical College of Wisconsin, Milwaukee, WI 53226, USA
Biochem Biophys Res Commun 317:384-9. 2004..Taken together, these findings implicate beta1Pix in regulating hexoasaminidase secretion and IP3 production in early stage upon mastoparan stimulation... - Endothelin-1 inhibits the epithelial Na+ channel through betaPix/14-3-3/Nedd4-2Tengis S Pavlov
Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
J Am Soc Nephrol 21:833-43. 2010..Taken together, these results suggest that the inhibitory effects of chronic ET-1 on ENaC result from betaPix interacting with the 14-3-3/Nedd4-2 pathway... - Endothelin 1 stimulates beta1Pix-dependent activation of Cdc42 through the G(salpha) pathwayAhmed Chahdi
Division of Nephrology, Department of Medicine, Kidney Disease Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226, USA
Exp Biol Med (Maywood) 231:761-5. 2006..These results demonstrate that beta1Pix, along with PKA, plays a crucial role in the regulation of Cdc42 activation by ET-1... - The role of beta(1)Pix/caveolin-1 interaction in endothelin signaling through Galpha subunitsAhmed Chahdi
Division of Nephrology and Kidney Disease Center, Department of Medicine, Medical College of Wisconsin, Milwaukee, WI 53226, United States
Biochem Biophys Res Commun 391:1330-5. 2010.... - CrkII associates with BCAR3 in response to endothelin-1 in human glomerular mesangial cellsVictoriya A Rufanova
Department of Medicine/Kidney Disease Center, Division of Nephrology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, Wisconsin 53226, USA
Exp Biol Med (Maywood) 231:752-6. 2006.... - Pyk2 mediates endothelin-1 signaling via p130Cas/BCAR3 cascade and regulates human glomerular mesangial cell adhesion and spreadingVictoriya A Rufanova
Division of Nephrology, Department of Medicine, Kidney Disease Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
J Cell Physiol 219:45-56. 2009..Our data suggest that ET-1 stimulated the GTPase Rap1 (but neither RhoA nor Ras) by a mechanism involving Pyk2 activation and recruitment of the p130Cas/BCAR3 complex in GMC... - Cyclooxygenase 2 inhibits SAPK activation in neuronal apoptosisBradley Miller
Department of Medicine, Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, WI 53226 0509, USA
Biochem Biophys Res Commun 300:884-8. 2003..Activation of SAPK in neuronal cell death was attenuated by DLC expression. These observations support a role for NO production and SAPK activation in the neuronal death mechanisms... - Endothelin-1 induces p66Shc activation through EGF receptor transactivation: Role of beta(1)Pix/Galpha(i3) interactionAhmed Chahdi
Kidney Disease Center, Department of Medicine, Medical College of Wisconsin, Milwaukee, 53226, United States
Cell Signal 22:325-9. 2010..Inhibition of Erk1/2 blocked p66Shc activation induced by ET-1. Altogether, our results indicate that ET-1 activates p66Shc through EGF receptor transactivation, leading to the activation of Galpha(i3), beta(1)Pix and Erk1/2... - Physiology and pathology of endothelin-1 in renal mesangiumAndrey Sorokin
Division of Nephrology, Medical College of Wisconsin, Milwaukee 53226, USA
Am J Physiol Renal Physiol 285:F579-89. 2003..In addition, we will discuss the status of clinical trials using ET antagonists, which have only been conducted in nonrenal disease, as a background for advocating their use in diseases characterized by excessive MC-derived ET-1... - Regulation of MDR-1 (P-glycoprotein) by cyclooxygenase-2Vimal A Patel
Department of Medicine, Division of Nephrology and the Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
J Biol Chem 277:38915-20. 2002..These results prove the existence of a causal link between Cox-2 and P-gp activity, which would have implications for kidney function and multidrug resistance in tumors where Cox-2 is overexpressed... - Cyclooxygenase-2 inhibits tumor necrosis factor alpha-mediated apoptosis in renal glomerular mesangial cellsAdiba Ishaque
Department of Medicine and Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee 53226, USA
J Biol Chem 278:10629-40. 2003..These results demonstrate COX-2 anti-apoptotic activity via a death receptor route and suggest that selective COX-2 inhibition may augment TNFalpha apoptosis in chronic inflammatory conditions... - Cyclooxygenase-2 rescues rat mesangial cells from apoptosis induced by adriamycin via upregulation of multidrug resistance protein 1 (P-glycoprotein)Bradley Miller
Department of Medicine, Cardiovascular Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
J Am Soc Nephrol 17:977-85. 2006..These data suggest that COX-2 protects GMC from ADR-mediated apoptosis via transcriptional upregulation of MDR-1 and that induced COX-2 expression would lessen ADR nephrotoxicity... - Cyclooxygenase 2 rescues LNCaP prostate cancer cells from sanguinarine-induced apoptosis by a mechanism involving inhibition of nitric oxide synthase activityJacob Huh
Department of Medicine and Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53266, USA
Cancer Res 66:3726-36. 2006.... - Arachidonic acid-induced carbon-centered radicals and phospholipid peroxidation in cyclo-oxygenase-2-transfected PC12 cellsJianfei Jiang
Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
J Neurochem 90:1036-49. 2004..Thus, COX-2 generated carbon-centered radicals but not oxygen radicals or thiyl radicals are responsible for oxidative stress in AA-challenged PC12 cells overexpressing COX-2... - The role of mitogen-activated protein kinase phosphatase-1 in the response of alveolar macrophages to lipopolysaccharide: attenuation of proinflammatory cytokine biosynthesis via feedback control of p38Qun Zhao
Center for Developmental Pharmacology and Toxicology, Children s Research Institute, Children s Hospital, Department of Pediatrics, The Ohio State University, Columbus, Ohio 43205, USA
J Biol Chem 280:8101-8. 2005..Our studies indicated that MKP-1 plays an important role in dampening the inflammatory responses of alveolar macrophages. We speculate that MKP-1 may represent a novel target for therapeutic intervention of inflammatory lung diseases... - ERK1/2-driven and MKP-mediated inhibition of EGF-induced ERK5 signaling in human proximal tubular cellsRita Sarközi
Clinical Division of Nephrology, Department of Internal Medicine, Medical University of Innsbruck, Innsbruck, Austria
J Cell Physiol 211:88-100. 2007..These results suggest the existence of an ERK1/2-driven negative feed-back regulation of ERK5 signaling in EGF-stimulated HK-2 cells, which is mediated by MKP-3, DUSP5 and/or MKP-1...
Research Grants
- ENDOTHELIN SIGNALING AND REGULATION OF PROTEIN KINASESAndrey Sorokin; Fiscal Year: 2006..We hypothesize that MKP-3 inhibits ET-1 mediated ERK activation, whereas MKP-1 attenuates p38 MAPK and tight regulation of MAPK signaling by MKP-1 and MKP-3 controls expression of genes critical for GMC hypertrophy. ..
- ENDOTHELIN SIGNALING AND REGULATION OF PROTEIN KINASESAndrey Sorokin; Fiscal Year: 2002..The phosphorylation status of glomerular adaptor proteins and protein kinases will be analyzed and correlated with the progression of the disease. ..
- CELLULAR REGULATION OF PROSTAGLANDIN SYNTHESISAndrey Sorokin; Fiscal Year: 2002..Here, PGHS-2 expression and prostaglandin production will be assessed in rat glomeruli and correlated with MAPK activation and the histopathology of GN at defined time points of the disease. ..
- CELLULAR REGULATION OF PROSTAGLANDIN SYNTHESISAndrey Sorokin; Fiscal Year: 2009....