Barbel Rohrer

Summary

Affiliation: Medical University of South Carolina
Country: USA

Publications

  1. ncbi request reprint Prolonged SRC kinase activation, a mechanism to turn transient, sublytic complement activation into a sustained pathological condition in retinal pigment epithelium cells
    Barbel Rohrer
    Department of Ophthalmology, Medical University of South Carolina, 167 Ashley Ave, SEI614, 29425, Charleston, SC, USA
    Adv Exp Med Biol 801:221-7. 2014
  2. pmc Systemic human CR2-targeted complement alternative pathway inhibitor ameliorates mouse laser-induced choroidal neovascularization
    Barbel Rohrer
    Department of Ophthalmology, Medical University of South Carolina, 167 Ashley Avenue, Charleston, SC 29425, USA
    J Ocul Pharmacol Ther 28:402-9. 2012
  3. ncbi request reprint Eliminating complement factor D reduces photoreceptor susceptibility to light-induced damage
    Barbel Rohrer
    Department of Neurosciences Division of Research, Medical University of South Carolina, Charleston, SC 29425, USA
    Invest Ophthalmol Vis Sci 48:5282-9. 2007
  4. pmc The alternative pathway is required, but not alone sufficient, for retinal pathology in mouse laser-induced choroidal neovascularization
    Barbel Rohrer
    Department of Ophthalmology, Medical University of South Carolina, Charleston, SC 29425, United States
    Mol Immunol 48:e1-8. 2011
  5. pmc A targeted inhibitor of the alternative complement pathway reduces angiogenesis in a mouse model of age-related macular degeneration
    Barbel Rohrer
    Department of Neurosciences, Division of Research, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Invest Ophthalmol Vis Sci 50:3056-64. 2009
  6. ncbi request reprint Rod and cone pigment regeneration in RPE65-/- mice
    Baerbel Rohrer
    Department of Ophthalmology, Medical University of South Carolina, Charleston, SC 29425, USA
    Adv Exp Med Biol 572:101-7. 2006
  7. pmc Classical complement activation and acquired immune response pathways are not essential for retinal degeneration in the rd1 mouse
    Barbel Rohrer
    Department of Ophthalmology, Medical University of South Carolina, Charleston, SC 29425, USA
    Exp Eye Res 84:82-91. 2007
  8. ncbi request reprint Cone opsin mislocalization in Rpe65-/- mice: a defect that can be corrected by 11-cis retinal
    Baerbel Rohrer
    Department of Ophthalmology, Medical University of South Carolina, Charleston, 29425, USA
    Invest Ophthalmol Vis Sci 46:3876-82. 2005
  9. pmc Functionally intact glutamate-mediated signaling in bipolar cells of the TRKB knockout mouse retina
    Baerbel Rohrer
    Department of Ophthalmology, Medical University of South Carolina, Charlston, SC 29425, USA
    Vis Neurosci 21:703-13. 2004
  10. ncbi request reprint Multidestructive pathways triggered in photoreceptor cell death of the rd mouse as determined through gene expression profiling
    Baerbel Rohrer
    Departments of Ophthalmology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 279:41903-10. 2004

Research Grants

  1. ROD NEURODEGENERATIVE AND PROTECTIVE GENES
    Baerbel Rohrer; Fiscal Year: 2001
  2. ROD NEURODEGENERATIVE AND PROTECTIVE GENES
    Baerbel Rohrer; Fiscal Year: 2002
  3. ROD NEURODEGENERATIVE AND PROTECTIVE GENES
    Baerbel Rohrer; Fiscal Year: 2002
  4. ROD NEURODEGENERATIVE AND PROTECTIVE GENES
    Baerbel Rohrer; Fiscal Year: 2003
  5. ROD NEURODEGENERATIVE AND PROTECTIVE GENES
    Baerbel Rohrer; Fiscal Year: 2004
  6. ROD NEURODEGENERATIVE AND PROTECTIVE GENES
    Baerbel Rohrer; Fiscal Year: 2003
  7. Transgenic Rod Development and Function
    Baerbel Rohrer; Fiscal Year: 2005
  8. ROD NEURODEGENERATIVE AND PROTECTIVE GENES
    Baerbel Rohrer; Fiscal Year: 2005
  9. Alternative Pathway of Complement Activation in Age-Related Macular Degeneration
    Baerbel Rohrer; Fiscal Year: 2010

Collaborators

Detail Information

Publications37

  1. ncbi request reprint Prolonged SRC kinase activation, a mechanism to turn transient, sublytic complement activation into a sustained pathological condition in retinal pigment epithelium cells
    Barbel Rohrer
    Department of Ophthalmology, Medical University of South Carolina, 167 Ashley Ave, SEI614, 29425, Charleston, SC, USA
    Adv Exp Med Biol 801:221-7. 2014
    ....
  2. pmc Systemic human CR2-targeted complement alternative pathway inhibitor ameliorates mouse laser-induced choroidal neovascularization
    Barbel Rohrer
    Department of Ophthalmology, Medical University of South Carolina, 167 Ashley Avenue, Charleston, SC 29425, USA
    J Ocul Pharmacol Ther 28:402-9. 2012
    ..We recently generated a factor H (fH)-based complement inhibitor (CR2-fH) with the capacity to be "targeted" to sites of complement C3 activation. We asked whether the human therapeutic (TT30) is effective in a mouse model of AMD...
  3. ncbi request reprint Eliminating complement factor D reduces photoreceptor susceptibility to light-induced damage
    Barbel Rohrer
    Department of Neurosciences Division of Research, Medical University of South Carolina, Charleston, SC 29425, USA
    Invest Ophthalmol Vis Sci 48:5282-9. 2007
    ..The authors tested the involvement of the complement system in an experimental model for oxidative stress-mediated photoreceptor degeneration, the light-damage mouse model...
  4. pmc The alternative pathway is required, but not alone sufficient, for retinal pathology in mouse laser-induced choroidal neovascularization
    Barbel Rohrer
    Department of Ophthalmology, Medical University of South Carolina, Charleston, SC 29425, United States
    Mol Immunol 48:e1-8. 2011
    ..Improving our understanding of the local regulation of this pathway in the eye is essential for developing improved treatment approaches for AMD...
  5. pmc A targeted inhibitor of the alternative complement pathway reduces angiogenesis in a mouse model of age-related macular degeneration
    Barbel Rohrer
    Department of Neurosciences, Division of Research, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Invest Ophthalmol Vis Sci 50:3056-64. 2009
    ..CR2-fH consists of the N terminus of mouse fH, which contains the AP-inhibitory domain, linked to a complement receptor 2 (CR2) targeting fragment that binds complement activation products...
  6. ncbi request reprint Rod and cone pigment regeneration in RPE65-/- mice
    Baerbel Rohrer
    Department of Ophthalmology, Medical University of South Carolina, Charleston, SC 29425, USA
    Adv Exp Med Biol 572:101-7. 2006
  7. pmc Classical complement activation and acquired immune response pathways are not essential for retinal degeneration in the rd1 mouse
    Barbel Rohrer
    Department of Ophthalmology, Medical University of South Carolina, Charleston, SC 29425, USA
    Exp Eye Res 84:82-91. 2007
    ..Our results suggest that both, the classical complement system of innate immunity and a functional acquired immune response are not essential for the degenerative process in the rd1 mouse retina...
  8. ncbi request reprint Cone opsin mislocalization in Rpe65-/- mice: a defect that can be corrected by 11-cis retinal
    Baerbel Rohrer
    Department of Ophthalmology, Medical University of South Carolina, Charleston, 29425, USA
    Invest Ophthalmol Vis Sci 46:3876-82. 2005
    ..This study was designed to investigate the feasibility of restoring functional cones with exogenous 11-cis retinal...
  9. pmc Functionally intact glutamate-mediated signaling in bipolar cells of the TRKB knockout mouse retina
    Baerbel Rohrer
    Department of Ophthalmology, Medical University of South Carolina, Charlston, SC 29425, USA
    Vis Neurosci 21:703-13. 2004
    ..These data indicate that there does not appear to be evidence for postsynaptic deficits in glutamatergic signaling in the ON and OFF bipolar cells of mice lacking trkB...
  10. ncbi request reprint Multidestructive pathways triggered in photoreceptor cell death of the rd mouse as determined through gene expression profiling
    Baerbel Rohrer
    Departments of Ophthalmology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 279:41903-10. 2004
    ..All of these components need to be addressed if rods are to be successfully protected...
  11. ncbi request reprint Neurotrophin receptor TrkB activation is not required for the postnatal survival of retinal ganglion cells in vivo
    B Rohrer
    Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, California 94143, USA
    Exp Neurol 172:81-91. 2001
    ..Thus, TrkB signaling is not required for survival of RGCs during the period of target-dependent survival, but does appear to reduce degeneration of RGCs in adult animals...
  12. ncbi request reprint Gene dosage effect of the TrkB receptor on rod physiology and biochemistry in juvenile mouse retina
    B Rohrer
    Storm Eye Institute, Department of Ophthalmology, Medical University of South Carolina, Charleston, SC 29425, USA
    Mol Vis 7:288-96. 2001
    ..To strengthen our understanding about the role of trkB in rod development by correlating functional and biochemical retinal phenotypes with levels of trkB expression in two independently created trkB transgenic lines...
  13. doi request reprint A targeted inhibitor of the complement alternative pathway reduces RPE injury and angiogenesis in models of age-related macular degeneration
    Barbel Rohrer
    Department of Ophthalmology, Medical University of South Carolina, Charleston, SC, USA
    Adv Exp Med Biol 703:137-49. 2010
    ..In both the in vivo and in vitro paradigm of RPE damage, a model requiring molecular events known to be involved in AMD, complement-dependent VEGF production, was confirmed. These data may open new avenues for AMD treatment strategies...
  14. ncbi request reprint Correlation of regenerable opsin with rod ERG signal in Rpe65-/- mice during development and aging
    Baerbel Rohrer
    Department of Ophthalmology Medical University of South Carolina, 167 Ashley Avenue, Charleston, SC 29425, USA
    Invest Ophthalmol Vis Sci 44:310-5. 2003
    ..This project was designed to determine the amount of regenerable opsin in Rpe65-/- mice during development and aging, and to examine the function of this rhodopsin by electroretinography (ERG)...
  15. ncbi request reprint Does constitutive phosphorylation protect against photoreceptor degeneration in Rpe65-/- mice?
    Baerbel Rohrer
    Department of Ophthalmology, Medical University of South Carolina, Charleston, SC, USA
    Adv Exp Med Biol 533:221-7. 2003
    ....
  16. ncbi request reprint Retarded outer segment development in TrkB knockout mouse retina organ culture
    Baerbel Rohrer
    Department of Ophthalmology, Medical University of South Carolina, Charleston, SC 29425, USA
    Mol Vis 9:18-23. 2003
    ..To determine the effects of trkB deficiency in the mouse retina on photoreceptor development and retinal organization, in the absence of confounding systemic effects...
  17. pmc Paradoxical role of BDNF: BDNF+/- retinas are protected against light damage-mediated stress
    R Brooks Wilson
    Department of Ophthalmology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Invest Ophthalmol Vis Sci 48:2877-86. 2007
    ..The authors hypothesized that retinas with reduced BDNF levels in which the BDNF-mediated release of neuroprotective signals is dampened are more susceptible to light-induced photoreceptor degeneration...
  18. ncbi request reprint Cone outer segment morphology and cone function in the Rpe65-/- Nrl-/- mouse retina are amenable to retinoid replacement
    Kannan Kunchithapautham
    Department of Neurosciences, Division of Research, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Invest Ophthalmol Vis Sci 50:4858-64. 2009
    ..Here, we determine the consequences of 11-cis retinal withdrawal and supplementation on cone development in the all-cone Nrl(-/-) retina...
  19. ncbi request reprint Multiple, parallel cellular suicide mechanisms participate in photoreceptor cell death
    Heather R Lohr
    Department of Neurosciences, Division of Research, Medical University of South Carolina, 173 Ashley Avenue, BSB403, Charleston, SC 29425, USA
    Exp Eye Res 83:380-9. 2006
    ..Thus, unless the common upstream initiator for a given photoreceptor dystrophy is found, multiple rescue paradigms need to be used to target all active pathways...
  20. ncbi request reprint 11-cis-retinal reduces constitutive opsin phosphorylation and improves quantum catch in retinoid-deficient mouse rod photoreceptors
    Zsolt Ablonczy
    Department of Ophthalmology, Medical University of South Carolina, 167 Ashley Avenue, Charleston, SC 29425, USA
    J Biol Chem 277:40491-8. 2002
    ..Our results indicate that opsin, which has not been exposed to 11-cis-retinal, does not generate the activity generally associated with the bleached apoprotein...
  21. pmc Explant cultures of Rpe65-/- mouse retina: a model to investigate cone opsin trafficking
    Mausumi Bandyopadhyay
    Department of Ophthalmology, Medical University of South Carolina, Charleston, SC, USA
    Mol Vis 19:1149-57. 2013
    ..Here, we asked whether explant cultures of Rpe65⁻/⁻ mouse retina are amenable to screening retinoids for their ability to promote opsin trafficking...
  22. pmc Identification of candidate genes for human retinal degeneration loci using differentially expressed genes from mouse photoreceptor dystrophy models
    Christina Demos
    Department of Physiology and Neuroscience, Medical University of South Carolina, Charleston, SC 29425, USA
    Mol Vis 14:1639-49. 2008
    ..Retinal degeneration (RD) is a complex mechanism that appears to involve many biologic processes including oxidative stress, apoptosis, and cellular remodeling. Currently there are 51 mapped, but not identified, RD human disease loci...
  23. ncbi request reprint Sustained elevation of intracellular cGMP causes oxidative stress triggering calpain-mediated apoptosis in photoreceptor degeneration
    Ashish K Sharma
    Department of Neurosciences, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Curr Eye Res 32:259-69. 2007
    ..These results establish a central role for oxidative stress in cGMP-induced cell death and suggest a ROS-mediated sequential activation of signal transduction events, which provide targets for future treatment strategies...
  24. ncbi request reprint Apoptosis and autophagy in photoreceptors exposed to oxidative stress
    Kannan Kunchithapautham
    Department of Neurosciences, Division of Research, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Autophagy 3:433-41. 2007
    ..And third, these results argue that the up-stream regulators of autophagy need to be identified as potential therapeutic targets in photoreceptor degeneration...
  25. pmc Role of neurotrophin receptor TrkB in the maturation of rod photoreceptors and establishment of synaptic transmission to the inner retina
    B Rohrer
    Howard Hughes Medical Institute, School of Medicine, University of California San Francisco, San Francisco, California 94143, USA
    J Neurosci 19:8919-30. 1999
    ..Moreover, inner retinal cells respond to exogenous BDNF with c-fos expression and extracellular signal-regulated kinase phosphorylation. Thus, interactions of rods with TrkB-expressing cells must be required for normal rod development...
  26. pmc Oxidative stress sensitizes retinal pigmented epithelial (RPE) cells to complement-mediated injury in a natural antibody-, lectin pathway-, and phospholipid epitope-dependent manner
    Kusumam Joseph
    Department of Ophthalmology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 288:12753-65. 2013
    ..Taken together, we have defined novel mechanisms of complement activation in oxidatively stressed RPE, linking molecular events involved in age-related macular degeneration, including the presence of natural antibodies and neoepitopes...
  27. pmc Differential effects of rapamycin on rods and cones during light-induced stress in albino mice
    Kannan Kunchithapautham
    Department of Neurosciences, Division of Research, Medical University of South Carolina, Charleston, USA
    Invest Ophthalmol Vis Sci 52:2967-75. 2011
    ....
  28. pmc Photoreceptor structure and function is maintained in organotypic cultures of mouse retinas
    Mausumi Bandyopadhyay
    Department of Neurosciences, Division of Research, Medical University of South Carolina, Charleston, SC 29425, USA
    Mol Vis 16:1178-85. 2010
    ..Here, we further examined rod and cone development, chromophore production by the RPE, and photoreceptor signaling to the inner retina under organ culture conditions...
  29. pmc Alternative complement pathway deficiency ameliorates chronic smoke-induced functional and morphological ocular injury
    Alex Woodell
    Division of Research, Department of Neurosciences, Medical University of South Carolina, Charleston, South Carolina, United States of America
    PLoS ONE 8:e67894. 2013
    ....
  30. pmc Sublytic membrane-attack-complex (MAC) activation alters regulated rather than constitutive vascular endothelial growth factor (VEGF) secretion in retinal pigment epithelium monolayers
    Kannan Kunchithapautham
    Department of Ophthalmology, Division of Research, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 286:23717-24. 2011
    ..Taken together, identifying how sublytic MAC induces VEGF expression and secretion might offer opportunities to selectively inhibit pathological VEGF release only...
  31. ncbi request reprint The complement regulatory protein CD59: insights into attenuation of choroidal neovascularization
    Gloriane Schnabolk
    Ralph H Johnson VA Medical Center, Division of Research, 29401, Charleston, SC, USA
    Adv Exp Med Biol 801:435-40. 2014
    ..Hence, this strategy might offer opportunities for site-specific inhibition of complement in diseases with restricted sites of inflammation such as AMD. ..
  32. ncbi request reprint A mechanistic review of cigarette smoke and age-related macular degeneration
    Alex Woodell
    Departments of Neurosciences, Division of Research, Medical University of South Carolina, 167 Ashley Ave, SEI 614, 29425, Charleston, SC, USA
    Adv Exp Med Biol 801:301-7. 2014
    ..Further exploration of the biological processes affected by cigarette smoke exposure will provide greater insight into the pathogenesis of AMD. ..
  33. pmc Early alterations in mitochondrial reserve capacity; a means to predict subsequent photoreceptor cell death
    Nathan R Perron
    Department of Pharmaceutical Sciences, Medical University of South Carolina, Charleston, SC 29425, USA
    J Bioenerg Biomembr 45:101-9. 2013
    ..In future experiments, we will explore these concepts for the development of neuroprotective agents...
  34. pmc Matrix metalloproteinase activity creates pro-angiogenic environment in primary human retinal pigment epithelial cells exposed to complement
    Mausumi Bandyopadhyay
    Department of Ophthalmology, Storm Eye Institute, Medical University of South Carolina, Charleston, South Carolina, USA
    Invest Ophthalmol Vis Sci 53:1953-61. 2012
    ..Here, we tested whether complement attack on RPE monolayers changes MMP secretion and activation, thereby altering the availability of growth factors in the extracellular space...
  35. ncbi request reprint Autophagy is one of the multiple mechanisms active in photoreceptor degeneration
    Kannan Kunchithapautham
    Department of Neurosciences, Division of Research, Medical University of South Carolina, 167 Ashley Avenue, Charleston, SC 29425, USA
    Autophagy 3:65-6. 2007
    ..Our investigation revealed that unless the common upstream initiator for a given photoreceptor dystrophy can be found, multiple rescue paradigms need to be used to target all active pathways...
  36. pmc Revealing functionally coherent subsets using a spectral clustering and an information integration approach
    Adam J Richards
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
    BMC Syst Biol 6:S7. 2012
    ..Here we report a principled approach for managing and integrating multiple data sources within the framework of graph-spectrum analysis in order to identify coherent gene subsets...
  37. ncbi request reprint The noradrenergic system of aged GDNF heterozygous mice
    V Zaman
    Department of Physiology and Neuroscience, Center on Aging, Medical University of South Carolina, Charleston, SC 29425, USA
    Cell Transplant 12:291-303. 2003
    ..These findings suggest that the LC-NE system of Gdnf+/- mice is impaired and suggest that GDNF plays an important role in continued maintenance of this neuronal system throughout life...

Research Grants9

  1. ROD NEURODEGENERATIVE AND PROTECTIVE GENES
    Baerbel Rohrer; Fiscal Year: 2001
    ..We expect to isolate common downstream target genes that are activated both during degeneration and neuroprotection. ..
  2. ROD NEURODEGENERATIVE AND PROTECTIVE GENES
    Baerbel Rohrer; Fiscal Year: 2002
    ..We expect to isolate common downstream target genes that are activated both during degeneration and neuroprotection. ..
  3. ROD NEURODEGENERATIVE AND PROTECTIVE GENES
    Baerbel Rohrer; Fiscal Year: 2002
    ..We expect to isolate common downstream target genes that are activated both during degeneration and neuroprotection. ..
  4. ROD NEURODEGENERATIVE AND PROTECTIVE GENES
    Baerbel Rohrer; Fiscal Year: 2003
    ..We expect to isolate common downstream target genes that are activated both during degeneration and neuroprotection. ..
  5. ROD NEURODEGENERATIVE AND PROTECTIVE GENES
    Baerbel Rohrer; Fiscal Year: 2004
    ..We expect to isolate common downstream target genes that are activated both during degeneration and neuroprotection. ..
  6. ROD NEURODEGENERATIVE AND PROTECTIVE GENES
    Baerbel Rohrer; Fiscal Year: 2003
    ..We expect to isolate common downstream target genes that are activated both during degeneration and neuroprotection. ..
  7. Transgenic Rod Development and Function
    Baerbel Rohrer; Fiscal Year: 2005
    ..However, more importantly, it will provide avenues for studying gene defects at the single cell level not only in the retina, but in other systems as well. ..
  8. ROD NEURODEGENERATIVE AND PROTECTIVE GENES
    Baerbel Rohrer; Fiscal Year: 2005
    ..We expect to isolate common downstream target genes that are activated both during degeneration and neuroprotection. ..
  9. Alternative Pathway of Complement Activation in Age-Related Macular Degeneration
    Baerbel Rohrer; Fiscal Year: 2010
    ..Our current research suggests that complement inhibitors may be very effective therapeutic agents, as they target proximal events prior to inflammation and CNV. ..