Research Topics
Species | KIRKWOOD A contact PRITCHARDSummaryAffiliation: Medical College of Wisconsin Country: USA Publications
Research Grants
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Detail Information
Publications
Effects of experimental asthma on inflammation and lung mechanics in sickle cell miceKirkwood A Pritchard
Department of Surgery, Division of Pediatric Surgery, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
Am J Respir Cell Mol Biol 46:389-96. 2012..SCD alone induces a baseline lung pathology that increases large and small airway resistance and primes the lungs to increased inflammation and airway hyperresponsiveness after OVA sensitization...
Surfactant protein D: not just for the lung anymoreKirkwood A Pritchard
Children's Research Institute, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
Am J Physiol Heart Circ Physiol 294:H1994. 2008- Chromium (VI) increases endothelial cell expression of ICAM-1 and decreases nitric oxide activityK A Pritchard
Department of Pathology, Pharmacology and Toxicology, Medical College of Wisconsin, The Cardiovascular Research Center, Milwaukee 53226, USA
J Environ Pathol Toxicol Oncol 19:251-60. 2000..Cr(VI)-induced increases in the adhesive properties of the endothelium may play a critical role in the initiation and progression of tissue injury through increased recruitment of proinflammatory white blood cells... - Hypoxia-induced acute lung injury in murine models of sickle cell diseaseKirkwood A Pritchard
Medical College of Wisconsin, Div of Pediatric Surgery, Cardiovascula Center M4060, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
Am J Physiol Lung Cell Mol Physiol 286:L705-14. 2004.... - Native low-density lipoprotein induces endothelial nitric oxide synthase dysfunction: role of heat shock protein 90 and caveolin-1Kirkwood A Pritchard
Department of Surgery, Division of Pediatric Surgery, Pharmacology and Toxicology, and Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
Free Radic Biol Med 33:52-62. 2002..These data support the hypothesis that n-LDL increases O(2)(*-), which scavenges *NO, and suggest that n-LDL uncouples eNOS activity by decreasing the association of hsp90 as an initial step in signaling eNOS to generate O(2)(*-)... - Heat shock protein 90 mediates the balance of nitric oxide and superoxide anion from endothelial nitric-oxide synthaseK A Pritchard
Division of Pediatric Surgery, Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
J Biol Chem 276:17621-4. 2001..These data show that Hsp90 is essential for eNOS-dependent.NO production and that inhibition of ATP-dependent conformational changes in Hsp90 uncouples eNOS activity and increases eNOS-dependent O(2) production... - [A comparison between L-4F and SC-4F in preventing low density lipoprotein induced endothelial cell dysfunction in cell culture]Zhi-jun Ou
Department of Cardiology, First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China
Zhonghua Xin Xue Guan Bing Za Zhi 33:411-4. 2005..It suggests that the specific structure of L-4F may play a crucial role in preventing atherosclerosis and it may provide a new clue for searching a novel approach on prevention and therapeutics of atherosclerosis in the future... - Increased resistance to myocardial ischemia in the Brown Norway vs. Dahl S rat: role of nitric oxide synthase and Hsp90Yang Shi
Division of Pediatric Surgery, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
J Mol Cell Cardiol 38:625-35. 2005..NO production and decreases uncoupled NOS3 activity. We conclude increased association of hsp90 with NOS3 is a major mechanism by which BN/Mcw hearts are more resistant to ischemia than SS/Mcw hearts... - Mechanisms of activation of eNOS by 20-HETE and VEGF in bovine pulmonary artery endothelial cellsYuenmu Chen
Pulmonary and Critical Care Division, Department of Medicine, Division of Pediatric Surgery and Childrens Research Institute, Cardiovascular Center Medical College of Wisconsin, 53226, USA
Am J Physiol Lung Cell Mol Physiol 291:L378-85. 2006..Unlike aortic endothelial cells, eNOS activation in BPAECs by either VEGF or 20-HETE does not appear to require increased association of Hsp90... - Endothelium-derived microparticles inhibit human cardiac valve endothelial cell functionDenise B Klinkner
Department of Surgery, Division of Pediatric Surgery, Medical College of Wisconsin and Children's Research Institute, Milwaukee, WI 53226, USA
Shock 25:575-80. 2006..These results implicate EMPs as a possible source of downstream EC dysfunction in disease states. EMPs may play a role in valvular leaflet injury in human disease by inhibiting normal growth and repair of endothelium... - Rosiglitazone antagonizes vascular endothelial growth factor signaling and nuclear factor of activated T cells activation in cardiac valve endotheliumTara L Sander
Department of Surgery, Medical College of Wisconsin and Children s Research Institute, Milwaukee, Wisconsin 53226, USA
Endothelium 13:181-90. 2006..These data provide the first demonstration that ROSI negatively regulates VEGF signaling in the valve endothelium by a mechanism involving NFATc1 activation and nuclear translocation... - BN phenome: detailed characterization of the cardiovascular, renal, and pulmonary systems of the sequenced ratAnne E Kwitek
Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
Physiol Genomics 25:303-13. 2006..These data provide a baseline for physiological comparison of traits related to cardiovascular, lung, blood, and renal function in the sequenced BN rats relative to the major strains of rats studied in biomedical research... - Effects of D-4F on vasodilation, oxidative stress, angiostatin, myocardial inflammation, and angiogenic potential in tight-skin miceDorothee Weihrauch
Cardiovascular Center, Medical College of Wisconsin, 8701 Watertown Plank Road, CVC M 4060, Milwaukee, WI 53226, USA
Am J Physiol Heart Circ Physiol 293:H1432-41. 2007..As SSc patients have increased plasma p-HDL and angiostatin levels similar to the Tsk(-/+) mice, D-4F may be effective at treating vascular complications in patients with SSc... - A heat shock protein 90 binding domain in endothelial nitric-oxide synthase influences enzyme functionHao Xu
Department of Surgery, Division of Pediatric Surgery, Medical College of Wisconsin, Children s Research Institute, Cardiovascular Research Center, Milwaukee, WI 53226, USA
J Biol Chem 282:37567-74. 2007..Such chaperone-dependent signaling may play an important role in modulating the balance of *NO and O(2)(*) generation from eNOS and, therefore, vascular function... - 20-hydroxyeicosatetraenoic acid causes endothelial dysfunction via eNOS uncouplingJennifer Cheng
Department of Pharmacology, New York Medical College, Valhalla, NY 10595, USA
Am J Physiol Heart Circ Physiol 294:H1018-26. 2008.... 
Comparative proteomic analysis of PAI-1 and TNF-alpha-derived endothelial microparticlesDanielle B Peterson
Department of Surgery, Medical College of Wisconsin and Children s Research Institute, Milwaukee, WI 53226, USA
Proteomics 8:2430-46. 2008..These observations provide fundamental insight into the mechanisms regulating the production of these particles and their physiological role in numerous diseases...- Histopathology of experimentally induced asthma in a murine model of sickle cell diseaseSandhya D Nandedkar
Department of Surgery, Division of Pediatric Surgery, Medical College of Wisconsin, Blood Research Institute, Children s Research Institute, Cardiovascular Center, Milwaukee, WI 53226, USA
Blood 112:2529-38. 2008..Our findings indicate SCD mice with experimentally induced asthma are more susceptible to death and pulmonary inflammation compared with control mice, suggesting that asthma contributes significantly to morbidity and mortality in SCD... - Targeted increases in endothelial cell superoxide anion production stimulate eNOS-dependent nitric oxide production, not uncoupled eNOS activityHao Xu
Arterioscler Thromb Vasc Biol 28:1580-1. 2008 - Endothelium-derived microparticles induce endothelial dysfunction and acute lung injuryJohn C Densmore
Department of Surgery, Medical College of Wisconsin and Children s Hospital of Wisconsin, 9000 W Wisconsin Avenue, Milwaukee, WI 53226, USA
Shock 26:464-71. 2006..Endothelium-derived microparticles inhibit endothelium-mediated vasodilation and *NO generation from eNOS. Once elucidated, EMP mechanisms of inducing ALI and endothelial dysfunction may present new therapeutic targets... - Vascular dysfunction in a murine model of severe hemolysisAnne C Frei
Blood Research Institute, Blood Center of Wisconsin, Milwaukee, Wisconsin, USA
Blood 112:398-405. 2008..Together, these data indicate that sph/sph mice with severe HS have increased plasma hemoglobin and NO scavenging capacity, likely contributing to aberrant vasoregulation and initiating oxidative damage... - Activation of protein kinases in chronically hypoxic infant human and rabbit hearts: role in cardioprotectionParvaneh Rafiee
Division of Pediatric Surgery, Medical College of Wisconsin, Milwaukee 53226, USA
Circulation 106:239-45. 2002..These pathways may be responsible for cardioprotection in the chronically hypoxic infant rabbit heart... - Native LDL and minimally oxidized LDL differentially regulate superoxide anion in vascular endothelium in situDavid W Stepp
Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
Am J Physiol Heart Circ Physiol 283:H750-9. 2002..As HSP90 mediates the balance of.NO and O. generation by eNOS, these data provide new insight into the mechanisms by which oxidative stress, induced by nLDL and mmLDL, uncouple eNOS activity to increase endothelial O. generation... - Chronic hypoxia increases endothelial nitric oxide synthase generation of nitric oxide by increasing heat shock protein 90 association and serine phosphorylationYang Shi
Division of Pediatric Surgery, Medical College of Wisconsin, Milwaukee, WI 53226, USA
Circ Res 91:300-6. 2002..Such changes in eNOS function appear to play a critical role in protecting the myocardium against ischemic injury... - Inhibition of heat shock protein 90 (hsp90) in proliferating endothelial cells uncouples endothelial nitric oxide synthase activityJingsong Ou
Department of Surgery, Division of Pediatric Surgery, Medical College of Wisconsin, Milwaukee, WI 53226, USA
Free Radic Biol Med 34:269-76. 2003..This study provides new understanding of the mechanisms by which ansamycin antibiotics inhibit endothelial cell proliferation. Such information may be useful in the development and design of new antineoplastic agents in the future... - Angiostatin: a negative regulator of endothelial-dependent vasodilationRyoji Koshida
Department of Physiology, and Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee 53226, USA
Circulation 107:803-6. 2003..Such changes in enzyme function begin to explain, in part, why angiostatin is antiangiogenic and impairs endothelium-dependent vasodilation... - L-4F, an apolipoprotein A-1 mimetic, restores nitric oxide and superoxide anion balance in low-density lipoprotein-treated endothelial cellsZhijun Ou
Department of Surgery, Medical College of Wisconsin, Children's Hospital of Wisconsin, Milwaukee 53226, USA
Circulation 107:1520-4. 2003..L-4F allows endothelial cell to maintain coupled eNOS activity to generate.NO even in the face of atherogenic concentrations of LDL... - Trichloroethylene decreases heat shock protein 90 interactions with endothelial nitric oxide synthase: implications for endothelial cell proliferationJingsong Ou
Department of Surgery, Division of Pediatric Surgery, Cardiovascular Center, Milwaukee, Wisconsin 53226, USA
Toxicol Sci 73:90-7. 2003..Such changes in endothelial function may play an important role in the development of congenital heart defects... - Decreased association of HSP90 impairs endothelial nitric oxide synthase in fetal lambs with persistent pulmonary hypertensionGirija G Konduri
Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
Am J Physiol Heart Circ Physiol 285:H204-11. 2003..Decreased HSP90-eNOS interactions may contribute to the impaired NO release and vasodilation observed in the ductal ligation model of PPHN... - L-4F, an apolipoprotein A-1 mimetic, dramatically improves vasodilation in hypercholesterolemia and sickle cell diseaseJingsong Ou
Division of Pediatric Surgery, Department of Surgery, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
Circulation 107:2337-41. 2003..Our findings suggest that L-4F restores vascular endothelial function in diverse models of disease and may be applicable to treating a variety of vascular diseases... - Mechanisms of endotoxin tolerance in human intestinal microvascular endothelial cellsHitoshi Ogawa
Division of Gastroenterology and Hepatology, Department of Surgery, Milwaukee Veterans Administration Medical Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
J Immunol 170:5956-64. 2003..HIMEC possess specific mechanisms of immunoregulatory hyporesponsiveness to repeated LPS exposure... - Cellular redistribution of inducible Hsp70 protein in the human and rabbit heart in response to the stress of chronic hypoxia: role of protein kinases,Parvaneh Rafiee
Division of Pediatric Surgery, Medical College of Wisconsin and Children's Hospital of Wisconsin, Milwaukee, Wisconsin 53226, USA
J Biol Chem 278:43636-44. 2003..We conclude the Hsp70 signal transduction pathway plays an important role in adaptation of infant human and rabbit hearts to chronic hypoxemia... - Phosphorylation of threonine 497 in endothelial nitric-oxide synthase coordinates the coupling of L-arginine metabolism to efficient nitric oxide productionMichelle I Lin
Department of Pharmacology and Program in Vascular Cell Signaling and Therapeutics, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536, USA
J Biol Chem 278:44719-26. 2003..Thus, the phosphorylation/dephosphorylation of Thr497 may be an intrinsic switch mechanism that determines whether eNOS generates NO versus superoxide in cells... - Heat shock protein 90 and tyrosine kinase regulate eNOS NO* generation but not NO* bioactivityJingsong Ou
Division of Pediatric Surgery, Department of Surgery, Cardiovascular Center, Medical College of Wisconsin, Milwaukee 53226, USA
Am J Physiol Heart Circ Physiol 286:H561-9. 2004..These data suggest that the tyrosine kinases, either directly or indirectly, and HSP90-dependent signaling pathways act in concert to suppress uncoupled eNOS activity... - Acute cardioprotective effects of erythropoietin in infant rabbits are mediated by activation of protein kinases and potassium channelsYang Shi
Pediatric Surgery, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
Basic Res Cardiol 99:173-82. 2004..We conclude the rapid activation of potassium channels and protein kinases by erythropoietin represents an important new mechanism for increasing cardioprotection... - AP-4F, antennapedia peptide linked to an amphipathic alpha helical peptide, increases the efficiency of Lipofectamine-mediated gene transfection in endothelial cellsJingsong Ou
Division of Pediatric Surgery, Department of Surgery, Medical College of Wisconsin, Cardiovascular Center M4060, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
Biochem Biophys Res Commun 305:605-10. 2003..These observations demonstrate that AP-4F may be useful for increasing the transfection efficiency of endothelial cell cultures with standard commercially available reagents... - Erythropoietin protects the infant heart against ischemia-reperfusion injury by triggering multiple signaling pathwaysParvaneh Rafiee
Medical College of Wisconsin, Division of Pediatric Surgery, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
Basic Res Cardiol 100:187-97. 2005..SB203580 inhibited only p38 MAPK activation by EPO. We can conclude EPO increases immediate cardioprotection through the activation of multiple signal transduction pathways... - Protein composition of plasminogen activator inhibitor type 1-derived endothelial microparticlesTara L Sander
Department of Surgery, Medical College of Wisconsin and Children s Research Institute, Milwaukee, Wisconsin 53226, USA
Shock 29:504-11. 2008..Important insight is provided into the generation and protein composition of PAI-1-derived EMPs... - Tetrahydrobiopterin in pulmonary hypertension: pulmonary hypertension in guanosine triphosphate-cyclohydrolase-deficient miceKirkwood A Pritchard
Circulation 111:2022-4. 2005 - Delayed cardioprotection with isoflurane: role of reactive oxygen and nitrogenYang Shi
Division of Pediatric Surgery, Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
Am J Physiol Heart Circ Physiol 288:H175-84. 2005..8% but not in 1.0% isoflurane-treated hearts. We conclude that isoflurane confers delayed cardioprotection in the adult rat, triggered by ROS and RNS... - Effects of D-4F on vasodilation and vessel wall thickness in hypercholesterolemic LDL receptor-null and LDL receptor/apolipoprotein A-I double-knockout mice on Western dietJingsong Ou
Division of Pediatric Surgery, Department of Surgery, Children's Research Institute, Medical College of Wisconsin, Milwaukee, WI 53226, USA
Circ Res 97:1190-7. 2005.... - Alpha phenyl-tert-butyl nitrone (PBN) protects syngeneic marrow transplant recipients from the lethal cytokine syndrome occurring after agonistic CD40 antibody administrationMaria Gendelman
Bone Marrow Transplant Program, Medical College of Wisconsin, Milwaukee, WI 53226, USA
Blood 105:428-31. 2005....
Research Grants
- HDL Dysfunction and Vascular InflammationKirkwood A Pritchard; Fiscal Year: 2010..Through these studies, new treatment modalities may be realized for preventing vascular dysfunction in a variety of diseases characterized by increases in oxidative stress and inflammation. ..
- Native LDL, Cholesterol and Impaired VasodilationKirkwood Pritchard; Fiscal Year: 2006..Investigations in to how 4F protects vascular function will provide new understanding of the mechanisms by which LDL impairs vasodilation and HDL protects vascular function. ..
- Hsp90 Mediates eNOS and Vascular FunctionKirkwood Pritchard; Fiscal Year: 2005..Findings from these studies will probably be relevant to and provide new understanding of mechanisms mediating vascular disease related to atherogenesis, hypertension and diabetes ..
- NATIVE LDL, CHOLESTEROL AND IMPAIRED VASORELAXATIONKirkwood Pritchard; Fiscal Year: 2002..The research combines the use of cell biology and vascular physiology to understand the biophysical and biochemical mechanisms by which cholesterol enrichment of the endothelium impairs vasorelaxation. ..
- Asthma Increases Vaso-occlusion in Sickle Cell DiseaseKIRKWOOD A contact PRITCHARD; Fiscal Year: 2010..If we are correct, then our drug therapies should improve blood vessel function and decrease airway resistance in SCD mice with experimental asthma. ..